Background and Aims
Pancreatic cancer risk is increased in Lynch syndrome (LS) patients with mismatch repair gene defects predisposing to colonic and extracolonic cancers with microsatellite instability (MSI). However, the frequency of MSI pancreatic cancers has never been ascertained in consecutive, unselected clinical series, and their contribution to the sporadic and inherited burden of pancreatic cancer remains to be established. Aims of the study were to determine the prevalence of MSI in surgically resected pancreatic cancers in a multicentric, retrospective study, and to assess the occurrence of pancreatic cancer in LS.
MS-status was screened by a panel of 5 mononucleotide repeats (Bat26, Bat25, NR-21, NR-24 and NR-27) in 338 consecutive pancreatic ductal adenocarcinoma (PDAC), resected at two Italian and one German referral centres. The personal history of pancreatic cancer was assessed in an independent set of 58 probands with LS and in 138 first degree relatives who had cancers.
Only one PDAC (0.3%) showed MSI. This was a medullary type cancer, with hMLH1-deficiency, and no identified germ-line mutation but methylation of hMLH1. Pancreatic cancer occurred in 5 (2.5%) LS patients. Histological sampling was available for 2 cases, revealing PDAC in one case and an ampullary cancer in the other one.
MSI prevalence is negligible in sporadic, resected PDAC. Differently, the prevalence of pancreatic cancer is 2.5% in LS patients, and cancers other than PDAC may be encountered in this setting. Surveillance for pancreatic cancer should be advised in LS mutation carriers at referral centers.
Citation: Laghi L, Beghelli S, Spinelli A, Bianchi P, Basso G, Di Caro G, et al. (2012) Irrelevance of Microsatellite Instability in the Epidemiology of Sporadic Pancreatic Ductal Adenocarcinoma. PLoS ONE 7(9): e46002. https://doi.org/10.1371/journal.pone.0046002
Editor: Antonio Moschetta, University of Bari & Consorzio Mario Negri Sud, Italy
Received: May 3, 2012; Accepted: August 23, 2012; Published: September 21, 2012
Copyright: © Laghi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research was funded by the Italian Minister of Health, grant “Tumor stroma interaction, as therapy target in pancreatic cancer” (Ricerca Finalizzata 2005, no. 58). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Despite ranking fourth for incidence, pancreatic ductal adenocarcinoma (PDAC) has the first mortality rate among gastrointestinal cancers . Knowledge of the molecular basis of PDAC, including genetically determined predispositions, increased steadily in the last decade. . The syndromes predisposing to PDAC have a tumor spectrum not restricted to the pancreas, and comprise melanoma in carriers of p16/CDNK2 germline mutations, and breast cancer in carriers of BRCA1 or of BRCA2 mutations. The risk of developing PDAC also increases in inherited predispositions to colorectal cancer, namely the Peutz-Jegers and the Lynch syndrome (LS, or Hereditary Non-Polyposis Hereditary Colorectal Cancer) , . LS is caused by germ-line mutations in one of the mismatch repair (MMR) genes hMLH1, hMSH2, and, less frequently, hMSH6 or PMS2. Cancers with MMR defects, whether arising in LS patients or sporadically, due to somatic hMLH1 hypermethylation, typically show the molecular phenotype of microsatellite instability (MSI) . MSI prevalence approaches 10% in colorectal cancer, LS accounting for one third of the cases and hMLH1 hypermethylation for the remaining part , . Typically, MSI cancers beside exhibiting peculiar pathological features such as medullary histology , have a lower pathological stage at diagnosis, and thus a better prognosis . MSI prevalence in gastric, uterine and ovarian cancers approaches that of colon cancer , . Considering the ominous prognosis of pancreatic cancer , , it would be relevant whether MSI testing could identify PDAC patients with better survival , . However, the prevalence of MSI remains undefined in pancreatic cancer.
Studies based upon the review of family history, found a risk of pancreatic cancer 7–8 times higher in LS families than in the general population , . On the other hand, a few studies assessed the prevalence of MSI in PDAC specimens. Goggins first reported 3 (3.7%) MSI cases in a North American series comprising 82 PDAC , while European studies from Poland  and Italy  did not find any MSI or MMR deficient PDAC in small surgical series. Studies of pre-selected cases detected MSI in 3 (8.6%) out of 35 PDAC from long-term (>3 years) survivors , and in 4 (22.0%) out of 18 PDAC with medullary histology . Rather differently, studies in unselected Japanese PDAC reported MSI rates above 10% ,  (Table 1). Notably, the MSI phenotype has been mainly found in K-RAS wild-type PDAC , .
Currently, the negative studies on MSI or MMR defects in PDAC ,  appear underpowered to detect significant difference (power<0.60 with 0.05 α-level vs. ref. 15). Thus, the relevance of MSI in PDAC remains questionable, due to the discrepancies among the studies and to the limited data available in an evidence-based perspective . Accordingly, the aim of our study was to assess the MSI prevalence in a large, multicentric and consecutive series of western European patients with pancreatic cancer, well characterized for pathological and molecular features, and for patient survival. In parallel, we also assessed the frequency of pancreatic tumors in a series of LS probands and of their first degree relatives retrieved from a colorectal cancer family clinic.
Prevalence and Clinico-pathological Features of MSI PDAC
Only 1 (0.3%) cancer out of 338 PDAC showed MSI in all the tested quasimonomorphic mononucleotide repeats (Bat25, Bat26, NR-21, NR-24 and NR-27). The other 337 sample did not show instability at any of the 5 markers (Figure 1, Panel A). The patient with MSI, hMLH1-deficient PDAC, was a 79 years old female, with no history of familiar cancer. The cancer, arisen in the pancreatic head, had a diameter of 4.5 cm, involving the distal pancreatic duct and the duodenum. This pT4N1b hMLH1-deficient cancer was poorly differentiated (Figure 1, Panel B), and angioinvasive. The patient died of post-surgical complications. No hMLH1 mutations were detected in DNA extracted from normal tissue retrieved from pathological specimens, but hMLH1 promoter showed hypermethylation. Cancer tissue harbored mutated K-RAScod12 and wild-type B-RAF. Furthermore, none of the 157 tested PDAC harbored B-RAFV600E mutation.
From top to bottom, an example of MS-Stable PDAC, and the only MSI PDAC (Case # 5) (Panel A). Immunohistochemical analysis of MMR protein expression in the medullary hMLH1-deficient PDAC (Case #5), retaining hMSH2 and hMSH6 expression. Note the loss of PMS2, due to protein degradation in the absence of the cognate partner hMLH1 (Objective magnification, 10×) (Panel B).
Prevalence of Pancreatic Tumors in LS
We identified 58 LS probands; 35 (60.3%) with hMSH2 defects, 20 (34.5%) with hMLH1 defects, and 3 (5.2%) with hMSH6 defects, plus 145 first degree relatives (35 in families with hMLH1, 98 in families with hMSH2, and 12 in families with hMSH6 defects) with a personal history of cancer. Out of a total of 203 LS patients, 5 (2.5%) had pancreatic tumors. The 5 tumors occurred in 5 families with hMSH2 defects, and 4 tumors were diagnosed below the age of 50 years. Tumor sampling was available for 2 cases from mutation carriers. In one case cytology obtained by fine-needle was diagnostic for PDAC, but no tissue was available for MS-status testing. The other case, treated by resection of the pancreatic head, was an ampullary cancer, with histological features of pancreato-biliary poorly-differentiated (G3) adenocarcinoma, showing MSI and hMSH2 deficiency.
MSI was an extremely rare event in the largest and only consecutive series of PDAC ever studied. The implications of our results involve assessment methods, pathological features, and clinical behavior of MSI PDAC as reported so far.
A debated issue has been the technical reproducibility and accuracy of the methods to test MS-status . Already the first proposed microsatellite panel was aimed to standardize MS-status assessment , although the di-nucleotide markers initially employed generate false positives , , while mononucleotides are specific ,  and can detect MSI  without matched normal tissue , . Noteworthy, the 5 employed markers were fully concordant in all MS-stable (or -unstable) cases. Accordingly, testing multiple mononucleotide repeats does not increase sensitivity and specificity in a cancer type in which MSI phenotype is so rarely encountered. At any event, our study is the only one performed by using a standard panel of 5 mononucleotide repeats , ,  on the largest series ever investigated. Accordingly, our method reflects the true prevalence of MSI cancers. MSI prevalence 20% such as reported by Nakata et al. using di-nucleotide markers only and not supported by immuno-histochemistry is questionable . However, MS-status assessment by mono-nucleotide markers detected a striking 13% prevalence of MSI PDAC, classified as sporadic, in Japanese patients . Thus, our data definitely establish that the prevalence of MSI PDAC in western countries does not exceed 1%, –, while further studies are needed to confirm in consecutive series a high prevalence of MSI PDAC in Japanese.
PDAC accounts for the lowest 5-year survival of any cancer. Although surgery is considered to offer the only chance of cure, the mean survival after resection is merely 18–20 months, and the 5-years survival rate does not exceed 20% (for stage I/II) in Western world , , . Differently, the survival rate in Japan is close to 40% –, and studies conducted in US revealed a longer survival for Asian patients with PDAC . A higher proportion of less advanced cancers in Asian than in Western patients , to which MSI cases might contribute, would explain this difference. Even though this might be the case in Japanese , in Western countries less then 10% of PDAC long survivors had MMR-deficient cancers , and our data rule out the possibility that MSI cases substantially contribute to improved survival. Finally, we cannot rule out a different MSI prevalence in advanced PDAC not amenable to surgical resection, currently the vast majority. However, due to the extremely poor survival of patients with advanced PDAC at diagnosis , the prevalence of MSI cases should be lower in these patients than in those undergoing surgery.
The only MSI and hMLH1-deficient PDAC which we detected showed hMLH1 promoter hypermethylation and was the only medullary cancer in our series. Following the description by Goggins and coll. of pancreatic medullary histology as a feature of MSI PDAC , others reported medullary MSI PDAC in patients with a family history consistent with LS , . Our case finding confirms that the medullary phenotype is a feature of MSI PDAC, irrespectively of inherited or sporadic origin , which occurs without BRAFV600E mutation in the latter . The lack of BRAFV600E mutation in our series also rules out any alternative role to K-RAS for this oncogene in pancreatic carcinogenesis, irrespectively of tumor MS-status .
As opposed to the rarity of sporadic MSI PDAC, pancreatic cancer would occur more frequently in in LS patients than in the general population. The 2.5% frequency of pancreatic tumors in our LS series was almost identical to the 3.0% of unspecified pancreatic cancer reported by Geary, a 7-fold higher occurrence than expected . Kastrinos calculated a 8.6 fold increased risk and 3.7% life-time risk of pancreatic cancer in LS families based upon family reports . Accordingly, germ-line MMR defects predispose to pancreatic cancer. One tumor in the Italian series of LS was a poorly differentiated pancreato-biliary subtype ampullary cancer, tipically associated with MSI . This occasional finding rises the hypothesis that tumors other than PDAC might contribute to the high rate of not histologically confirmed pancreatic cancer in LS. At any event, pancreatic surveillance for neoplasms in carriers of MMR gene mutation should be advised in referral centers, especially for LS families with a positive history of pancreatic tumors , . A similar approach aimed to increase the rate of early diagnosis, and thus of surgical management, will also provide insights on the survival gain due to the proper recognition and treatment of pancreatic cancer in the setting of LS.
Materials and Methods
For the retrospective study, a total of 338 consecutive specimens from Europeans patients who had undergone pancreatic resection for PDAC at three academic institutions were retrieved. The series included 181 PDAC from the University Hospital of Verona, 91 from the Humanitas Clinical and Research Center, Rozzano, University of Milan, and 66 from the Charitè Campus Virchow, University of Berlin. Patient demographics and survival, family history of gastrointestinal cancer, and tumor pathological and molecular features of the PDAC series are detailed in Table 2.
The written informed consent of patients to the handling of clinical data had been obtained by the referring physicians at admission for surgery. Being the study at no risk to patients and their privacy, the approval for the use of pathology specimens with a waiver of consent was granted by the Review Board of the Humanitas Clinical and Research Center and of the Charitè Campus Virchow, and by the Ethics Committees of the University Hospital Trust of Verona. A coded data-base was prepared by clinical researchers unaware of molecular data, and deidentified samples under code were obtained from the pathology archives for molecular analysis.
MS-status Assessment and Analysis of MMR Defects
DNA was extracted from 5 micron thick, paraffin-embedded specimens, and cancer tissue was micro-dissected if tumor cells did not account for at least 50% of the sample. MSI assignment was based on the analysis of mononucleotide repeats. After DNA extraction by proteinase-K digestion and phenol-chloroform purification, amplification of the mononucleotide microsatellites BAT25, BAT26, NR-21, NR-24 and NR-27 with fluorescent dye-labeled primers was followed by capillary-gel electrophoresis (ABI PRISM 310 DNA Sequencer, Perkin-Elmer, Foster City, CA, USA) , , , , .
hMLH1 and hMSH2 MMR protein defects were tested by immunohistochemistry in the Verona series, as well as in MSI cases identified by molecular testing. hMLH1 (G-168 monoclonal antibody, PharMingen; San Diego, CA, US), hMSH2 (clone FE 11, Oncogene Sciences; Cambridge, MA, US), hMSH6 (clone 44, Transduction Laboratories), and PMS2 (clone, A16-4, PharMingen) were tested according to previously described methods . Lesions were considered negative in the complete absence of detectable nuclear staining in neoplastic cells. In MSI cases, sequencing of defective MMR genes, according to the protein defect, was performed. Exons and intron/exon boundaries of the defective gene were amplified according to previously described techniques .
Mutations at codon 12 of KRAS were detected by PCR–RFLP using a modified primer that creates a restriction site for BstNI (New England Biolabs Inc., Beverly, MA, USA) restriction enzymes .
The mutational status of B-RAF codon 600 vas determined RT-PCR using a TaqMan SNP Genotyping Assay (Applied Biosystem) in 157 (44.1%) PDAC specimens, representing all consecutive cases collected from the Universities of Milan and Berlin. TaqMan MGB probes were designed using the Custom TaqMan Assay Design Tool (Applied Biosystem). The chosen reporter fluorophores were VIC for detecting the wild type allele and FAM for the mutant allele (Table 3).
Methylation of hMLH1 CpG islands was determined by treatment with sodium bisulfite and PCR, using specific primers for methylated and unmethylated DNA . PCRs were performed with positive methylation control, a human placental DNA treated in vitro with excess of SssI methyltransferase that generates a completely methylated DNA.
Frequency of Pancreatic Tumors in LS Patients
The occurrence of tumors arising in the pancreas was retrospectively assessed in 58 LS probands with known MMR gene mutations and in 145 first degree relatives for whom a personal history of cancer was available at the Colorectal Cancer Family Clinic of the Humanitas Clinical and Research Center.
The LS series did not overlap with the surgical PDAC series of the same institution.
Conceived and designed the experiments: LL A. Spinelli PB A. Scarpa AM. Performed the experiments: S. Beghelli PB GB GDC AB GC GT S. Bersani. Analyzed the data: LL A. Spinelli AB GDC PB A. Scarpa AM. Contributed reagents/materials/analysis tools: GS CR IG MR AZ PN CB MM A. Scarpa. Wrote the paper: LL PB GDC.
- 1. Katz MH, Hwang R, Fleming JB, Evans DB (2008) Tumor-node-metastasis staging of pancreatic adenocarcinoma. CA Cancer J Clin 58: 111–125.
- 2. Hidalgo M (2010) Pancreatic cancer. N Engl J Med 362: 1996;1978:1986–1990.1996;1605–1917.
- 3. Grover S, Syngal S (2010) Hereditary pancreatic cancer. Gastroenterology 139: 1076–1080, 1080 e1071–1072.
- 4. Laghi L, Bianchi P, Malesci A (2008) Differences and evolution of the methods for the assessment of microsatellite instability. Oncogene 27: 6313–6321.
- 5. Malesci A, Laghi L, Bianchi P, Delconte G, Randolph A, et al. (2007) Reduced likelihood of metastases in patients with microsatellite-unstable colorectal cancer. Clin Cancer Res 13: 3831–3839.
- 6. Alexander J, Watanabe T, Wu TT, Rashid A, Li S, et al. (2001) Histopathological identification of colon cancer with microsatellite instability. Am J Pathol 158: 527–535.
- 7. Halling KC, Harper J, Moskaluk CA, Thibodeau SN, Petroni GR, et al. (1999) Origin of microsatellite instability in gastric cancer. Am J Pathol 155: 205–211.
- 8. Murphy MA, Wentzensen N (2010) Frequency of mismatch repair deficiency in ovarian cancer: A systematic review. Int J Cancer.
- 9. DiMagno EP, Reber HA, Tempero MA (1999) AGA technical review on the epidemiology, diagnosis, and treatment of pancreatic ductal adenocarcinoma. American Gastroenterological Association. Gastroenterology 117: 1464–1484.
- 10. Carpelan-Holmstrom M, Nordling S, Pukkala E, Sankila R, Luttges J, et al. (2005) Does anyone survive pancreatic ductal adenocarcinoma? A nationwide study re-evaluating the data of the Finnish Cancer Registry. Gut 54: 385–387.
- 11. Nakata B, Wang YQ, Yashiro M, Nishioka N, Tanaka H, et al. (2002) Prognostic value of microsatellite instability in resectable pancreatic cancer. Clin Cancer Res 8: 2536–2540.
- 12. Yamamoto H, Itoh F, Nakamura H, Fukushima H, Sasaki S, et al. (2001) Genetic and clinical features of human pancreatic ductal adenocarcinomas with widespread microsatellite instability. Cancer Res 61: 3139–3144.
- 13. Geary J, Sasieni P, Houlston R, Izatt L, Eeles R, et al. (2008) Gene-related cancer spectrum in families with hereditary non-polyposis colorectal cancer (HNPCC). Fam Cancer 7: 163–172.
- 14. Kastrinos F, Mukherjee B, Tayob N, Wang F, Sparr J, et al. (2009) Risk of pancreatic cancer in families with Lynch syndrome. Jama 302: 1790–1795.
- 15. Goggins M, Offerhaus GJ, Hilgers W, Griffin CA, Shekher M, et al. (1998) Pancreatic adenocarcinomas with DNA replication errors (RER+) are associated with wild-type K-ras and characteristic histopathology. Poor differentiation, a syncytial growth pattern, and pushing borders suggest RER+. Am J Pathol 152: 1501–1507.
- 16. Tomaszewska R, Okon K, Stachura J (2003) Expression of the DNA mismatch repair proteins (hMLH1 and hMSH2) in infiltrating pancreatic cancer and its relation to some phenotypic features. Pol J Pathol 54: 31–37.
- 17. Ghimenti C, Tannergard P, Wahlberg S, Liu T, Giulianotti PG, et al. (1999) Microsatellite instability and mismatch repair gene inactivation in sporadic pancreatic and colon tumours. Br J Cancer 80: 11–16.
- 18. Maple JT, Smyrk TC, Boardman LA, Johnson RA, Thibodeau SN, et al. (2005) Defective DNA mismatch repair in long-term (> or = 3 years) survivors with pancreatic cancer. Pancreatology 5: 220–227; discussion 227–228.
- 19. Wilentz RE, Goggins M, Redston M, Marcus VA, Adsay NV, et al. (2000) Genetic, immunohistochemical, and clinical features of medullary carcinoma of the pancreas: A newly described and characterized entity. Am J Pathol 156: 1641–1651.
- 20. Real FX (2005) Pancreatic Ductal Adenocarcinoma: Microsatellite Instability, familial Cancer Syndromes, and Medullary Histology. Pancreatology 5: 227–228.
- 21. Boland CR, Thibodeau SN, Hamilton SR, Sidransky D, Eshleman JR, et al. (1998) A National Cancer Institute Workshop on Microsatellite Instability for cancer detection and familial predisposition: development of international criteria for the determination of microsatellite instability in colorectal cancer. Cancer Res 58: 5248–5257.
- 22. Perucho M (1999) Correspondence re: C.R. Boland, et al., A National Cancer Institute workshop on microsatellite instability for cancer detection and familial predisposition: development of international criteria for the determination of microsatellite instability in colorectal cancer. Cancer Res., 58: 5248–5257, 1998. Cancer Res 59: 249–256.
- 23. Xicola RM, Llor X, Pons E, Castells A, Alenda C, et al. (2007) Performance of different microsatellite marker panels for detection of mismatch repair-deficient colorectal tumors. J Natl Cancer Inst 99: 244–252.
- 24. Percesepe A, Kristo P, Aaltonen LA, Ponz de Leon M, de la Chapelle A, et al. (1998) Mismatch repair genes and mononucleotide tracts as mutation targets in colorectal tumors with different degrees of microsatellite instability. Oncogene 17: 157–163.
- 25. Nardon E, Glavac D, Benhattar J, Groenen PJ, Hofler G, et al. (2010) A multicenter study to validate the reproducibility of MSI testing with a panel of 5 quasimonomorphic mononucleotide repeats. Diagn Mol Pathol 19: 236–242.
- 26. AGA (1999) American gastroenterological association medical position statement: epidemiology, diagnosis, and treatment of pancreatic ductal adenocarcinoma. Gastroenterology 117: 1463–1484.
- 27. Nagakawa T, Konishi I, Ueno K, Ohta T, Akiyama T, et al. (1991) Surgical treatment of pancreatic cancer. The Japanese experience. Int J Pancreatol 9: 135–143.
- 28. Nagakawa T, Nagamori M, Futakami F, Tsukioka Y, Kayahara M, et al. (1996) Results of extensive surgery for pancreatic carcinoma. Cancer 77: 640–645.
- 29. Onoyama H, Kamigaki T, Yamamoto M, Saitoh Y (1992) [Treatment and present status of pancreatic cancer]. Gan To Kagaku Ryoho 19: 2304–2310.
- 30. Furukawa H, Okada S, Saisho H, Ariyama J, Karasawa E, et al. (1996) Clinicopathologic features of small pancreatic adenocarcinoma. A collective study. Cancer 78: 986–990.
- 31. Longnecker DS, Karagas MR, Tosteson TD, Mott LA (2000) Racial differences in pancreatic cancer: comparison of survival and histologic types of pancreatic carcinoma in Asians, blacks, and whites in the United States. Pancreas 21: 338–343.
- 32. Zell JA, Rhee JM, Ziogas A, Lipkin SM, Anton-Culver H (2007) Race, socioeconomic status, treatment, and survival time among pancreatic cancer cases in California. Cancer Epidemiol Biomarkers Prev 16: 546–552.
- 33. Banville N, Geraghty R, Fox E, Leahy DT, Green A, et al. (2006) Medullary carcinoma of the pancreas in a man with hereditary nonpolyposis colorectal cancer due to a mutation of the MSH2 mismatch repair gene. Hum Pathol 37: 1498–1502.
- 34. Calhoun ES, Jones JB, Ashfaq R, Adsay V, Baker SJ, et al. (2003) BRAF and FBXW7 (CDC4, FBW7, AGO, SEL10) mutations in distinct subsets of pancreatic cancer: potential therapeutic targets. Am J Pathol 163: 1255–1260.
- 35. Immervoll H, Hoem D, Kugarajh K, Steine SJ, Molven A (2006) Molecular analysis of the EGFR-RAS-RAF pathway in pancreatic ductal adenocarcinomas: lack of mutations in the BRAF and EGFR genes. Virchows Arch 448: 788–796.
- 36. Ruemmele P, Dietmaier W, Terracciano L, Tornillo L, Bataille F, et al. (2009) Histopathologic features and microsatellite instability of cancers of the papilla of vater and their precursor lesions. Am J Surg Pathol 33: 691–704.
- 37. Suraweera N, Duval A, Reperant M, Vaury C, Furlan D, et al. (2002) Evaluation of tumor microsatellite instability using five quasimonomorphic mononucleotide repeats and pentaplex PCR. Gastroenterology 123: 1804–1811.
- 38. Laghi L, Ranzani GN, Bianchi P, Mori A, Heinimann K, et al. (2002) Frameshift mutations of human gastrin receptor gene (hGARE) in gastrointestinal cancers with microsatellite instability. Lab Invest 82: 265–271.
- 39. Miranda E, Destro A, Malesci A, Balladore E, Bianchi P, et al. (2006) Genetic and epigenetic changes in primary metastatic and nonmetastatic colorectal cancer. Br J Cancer 95: 1101–1107.
- 40. Herman JG, Graff JR, Myohanen S, Nelkin BD, Baylin SB (1996) Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci U S A 93: 9821–9826.