Human DNA polymerase kappa (pol κ) is a translesion synthesis (TLS) polymerase that catalyzes TLS past various minor groove lesions including N2-dG linked acrolein- and polycyclic aromatic hydrocarbon-derived adducts, as well as N2-dG DNA–DNA interstrand cross-links introduced by the chemotherapeutic agent mitomycin C. It also processes ultraviolet light-induced DNA lesions. Since pol κ TLS activity can reduce the cellular toxicity of chemotherapeutic agents and since gliomas overexpress pol κ, small molecule library screens targeting pol κ were conducted to initiate the first step in the development of new adjunct cancer therapeutics. A high-throughput, fluorescence-based DNA strand displacement assay was utilized to screen ∼16,000 bioactive compounds, and the 60 top hits were validated by primer extension assays using non-damaged DNAs. Candesartan cilexetil, manoalide, and MK-886 were selected as proof-of-principle compounds and further characterized for their specificity toward pol κ by primer extension assays using DNAs containing a site-specific acrolein-derived, ring-opened reduced form of γ-HOPdG. Furthermore, candesartan cilexetil could enhance ultraviolet light-induced cytotoxicity in xeroderma pigmentosum variant cells, suggesting its inhibitory effect against intracellular pol κ. In summary, this investigation represents the first high-throughput screening designed to identify inhibitors of pol κ, with the characterization of biochemical and biologically relevant endpoints as a consequence of pol κ inhibition. These approaches lay the foundation for the future discovery of compounds that can be applied to combination chemotherapy.
Citation: Yamanaka K, Dorjsuren D, Eoff RL, Egli M, Maloney DJ, Jadhav A, et al. (2012) A Comprehensive Strategy to Discover Inhibitors of the Translesion Synthesis DNA Polymerase κ. PLoS ONE 7(10): e45032. doi:10.1371/journal.pone.0045032
Editor: John R. Battista, Louisiana State University and A & M College, United States of America
Received: June 6, 2012; Accepted: August 11, 2012; Published: October 8, 2012
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Funding: This work was supported by National Institute of Environmental Health Sciences [ES05355 to R.S.L.], National Cancer Institute [CA106858 to R.S.L.], National Institute of Mental Health [R03 MH 094179 to R.S.L.], and National Institute of General Medical Sciences [R00 GM 084460 to R.L.E.]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Cells employ multiple mechanisms to repair or tolerate DNA lesions in order to maintain genomic integrity. Translesion DNA synthesis (TLS) is one of the mechanisms used to tolerate unrepaired DNA lesions –. DNA polymerase κ (pol κ) is a TLS polymerase that has been shown to catalyze TLS past a variety of DNA lesions, being particularly proficient in the bypass of minor groove N2-dG lesions, including the acrolein-derived adducts γ-HOPdG and its ring-opened reduced form, DNA–peptide cross-links, and DNA–DNA interstrand cross-links (ICLs), as well as adducts induced by activated polycyclic aromatic hydrocarbons such as benzo[a]pyrene diolepoxide –. Importantly, pol κ has been demonstrated to be involved in the tolerance of ICLs induced by a chemotherapeutic agent, mitomycin C . In addition to its role in the bypass of N2-dG lesions, pol κ has also been shown to play a role in the processing of various ultraviolet (UV) light-induced DNA lesions –.
Many clinically relevant chemotherapeutic agents, including mitomycin C, cisplatin, and nitrogen mustard, target tumor cells by virtue of their ability to covalently cross-link complementary DNA strands, introducing ICLs into the genome. These ICL-inducing agents are powerful chemotherapeutic agents as the ICL interferes with vital cellular processes such as DNA replication, RNA transcription, and recombination by preventing transient DNA strand separation –. Therefore, although TLS is an essential process for cells to survive genotoxic stress, the ability of pol κ to bypass ICLs could limit the efficacy of these agents. Critical to this point are data demonstrating that the effectiveness of mitomycin C was increased when pol κ expression was suppressed by siRNA .
Germane to these observations, previous reports have suggested that pol κ may play a role in glioma development and therefore serve as a potential target for novel routes of therapies. Gliomas are the most common form of primary brain cancer and represent what is currently a generally incurable tumor in humans. These tumors are highly resistant to current treatment strategies, including chemotherapy with alkylating agents such as temozolomide, leading to median survival of patients with high-grade gliomas of only 1 year post diagnosis . Therefore, there is an urgent need for development of new therapies. Significantly, the level of pol κ has been shown to be upregulated in tumors from glioma patients, with its level being highly correlated with the grades of disease. Moreover, glioma patients expressing high levels of pol κ have an even poorer prognosis . Collectively, these data suggest a potential role for pol κ in the development of glioma. Thus, the identification of small molecule inhibitors targeting pol κ may be crucial for improving the therapeutic efficacy of chemotherapeutic agents.
To the best of our knowledge, only one selective small molecule inhibitor of pol κ has been identified to date: a natural product, 3-O-methylfunicone . This compound exhibits selectivity against Y-family DNA polymerases, and importantly, among the Y-family polymerases investigated, it shows the highest potency towards pol κ at IC50 of 12.5 µM. However, the utilization of 3-O-methylfunicone for therapeutic purposes is limited by its low potency. Additionally, given a lack of analogues and structure-activity relationship of this compound, it is unclear whether 3-O-methylfunicone-based agents can be developed into efficient therapeutics. Thus, in search for compounds with improved potency, a quantitative high-throughput screening (qHTS) of libraries of bioactive molecules composed of 15,805 members was carried out. Here we report the new strategies to identify small molecule inhibitors of pol κ.
Materials and Methods
1 M Tris-HCl was purchased from Invitrogen (Grand Island, NY), while Tween-20, KCl, MgCl2, and dithiothreitol (DTT) were purchased from Sigma–Aldrich (St. Louis, MO). Black 384-well and 1,536-well plates were purchased from Greiner Bio-One (Monroe, NC). [γ-32P]ATP was obtained from PerkinElmer Life Sciences (Waltham, MA). P-6 Bio-Spin columns were obtained from Bio-Rad (Hercules, CA). T4 polynucleotide kinase and 100 bp DNA ladder were purchased from New England BioLabs (Beverly, MA). Human pol κ (residues 19–526) was purified as previously reported . Human pol η (residues1–437) was purified following the same procedures as the purification of pol κ. Human pol ι was purchased from Enzymax Inc. (Lexington, KY). Yeast pol δ was a generous gift from Dr. Peter M. J. Burgers (Washington University, St. Louis, MO). Dimethyl sulfoxide (DMSO, certified ACS grade), paraformaldehyde, glacial acetic acid, crystal violet, and ethidium bromide were purchased from Fisher Scientific (Pittsburgh, PA). Clear 96-well plates and white/clear bottom 96-well plates were purchased from BD Falcon (Franklin Lakes, NJ) and Corning (Corning, NY), respectively. CellTiter-Glo Luminescent Cell Viability Assay was obtained from Promega (Madison, WI). Xeroderma pigmentosum variant (XP-V), XP30RO cells were generously supplied by Dr. James E. Cleaver (University of California, San Francisco, San Francisco, CA) and were generated as described previously .
The three oligodeoxynucleotides used in the qHTS (see below under qHTS) were purchased from Biosearch Technologies, Inc., (Novato, CA). Control unadducted oligodeoxynucleotides and an oligodeoxynucleotide adducted with acrolein-derived ring-opened reduced form of γ-HOPdG were the generous gifts of Dr. Carmelo J. Rizzo (Vanderbilt University, Nashville, TN).
The substrate used in qHTS was made by the annealing of unlabeled oligodeoxynucleotide primer (5′-TCACCCTCGTACGACTC-3′) and TAMRA-labeled reporter strand (5′-TTTTTTTTTTGC-6-TAMRA-3′) to oligodeoxynucleotide template labeled with BHQ-2 (5′-BHQ-2-GCAAAAAAAAAAGAGTCGTACGAGGGTGA-3′) as originally described by Dorjsuren et al. . The annealing mixture of unlabeled primer, TAMRA-labeled reporter, and BHQ-2-labeled template in buffer containing 50 mM Tris-HCl, pH 8.0, 100 mM NaCl, and 5 mM MgCl2 was heated at 95°C for 5 min and allowed to cool gradually to room temperature. The substrate was then stored at −20°C as 50 µM stock.
The screening collection of 15,805 members included the following libraries with the number of compounds indicated in parentheses: NCGC Pharmaceutical Collection (NPC) , MicroSource Spectrum collection (2,031), TimTec natural products (400), the LOPAC1280 collection from Sigma–Aldrich (1280), Tocris (1624), Prestwick (1597), BioMol (1943), Pharmacopeia (1648), and NCGC chemistry analogues and several additional small-size collections of bioactives.
qHTS assay protocol.
qHTS was performed in 50 mM Tris-HCI, pH 8.0, 40 mM NaCl, 1 mM MgCl2, 0.01% Tween-20, 2 mM DTT, and 100 µM dTTP. 3 µL of reagents (buffer as negative control and pol κ in the remaining plate at 10 nM final concentration) were dispensed by Flying Reagent Dispenser™ (FRD) (Beckman Coulter, Inc., Fullerton, CA) into a 1,536-well plate. Compounds were delivered as 23 nL in DMSO solution via pintool transfer; vehicle-only control consisted of 23 nL DMSO. The plate was incubated for 15 min at room temperature, and then 1 µL of substrate (50 nM final concentration) was added to initiate the reaction. The plate was immediately transferred into ViewLux reader for kinetic fluorescence data collection.
Radioactive gel-based primer extension assays
The reactions with pol κ, pol η, and pol ι were carried out in the buffers containing 25 mM Tris-HCl, pH 7.5, 8 mM MgCl2, 10% glycerol, 100 µg/mL bovine serum albumin, and 5 mM DTT. Buffers for pol δ reactions contained 40 mM Hepes-KOH, pH 6.8, 10% glycerol, 200 µg/mL bovine serum albumin, 1 mM DTT, and 8 mM MgCl2. Final concentration of pol κ, pol η, pol ι, or pol δ in the reaction was 0.25 nM, 1 nM, 2.5 nM, or 4 nM, respectively. In this assay, a polymerase was preincubated with the compound for 15 min at room temperature. Primer extensions were then initiated by the addition of DNA substrate at final concentration of 5 nM and carried out for 30 min at room temperature in the presence of 100 µM dCTP and dGTP. The reactions were terminated by the addition of an equal volume of a dye solution containing 95% (v/v) formamide, 10 mM EDTA, 0.03% (w/v) xylene cyanol, and 0.03% (w/v) bromphenol blue. Reaction products were separated through a 15% acrylamide gel containing 8 M urea and visualized with a PhosphorImager screen. The ratio of extended primer to the total amount of primer was measured using ImageQuant 5.2 software to calculate the percentage of primer extended. IC50 values were determined by fitting the data to variable slope four-parameter equations using GraphPad Prism 5.
DNA intercalation assay
Candesartan cilexetil or ethidium bromide was incubated with 1.5 nmol of double-stranded DNA ladder for 15 min at room temperature in the buffer containing 50 mM Tris-HCl, pH 8.0, 0.01% Tween-20, 1 mM DTT, and 40 mM NaCl. The reaction mixtures were run on 1% agarose gel and stained with ethidium bromide (0.5 µg/ml) for visualization. The amounts of candesartan cilexetil or ethidium bromide used for the reactions were indicated in figure legends.
Cell survival assays
Cell survival assays were carried out using crystal violet assays and CellTiter-Glo Luminescent Cell Viability Assays. In crystal violet assays, XP30RO cells were plated into clear 96-well plates at a density of 8000 cells/mL and incubated overnight at 37°C, 5% CO2. The cells were treated with (1) inhibitor alone for 6 h or (2) UV alone or (3) inhibitor alone for 6 h followed by UV irradiation. After 2 days, cells were fixed with 4% paraformaldehyde and stained with 0.5% crystal violet dye. The dye was dissolved in 10% acetic acid and absorbance measurement was taken at OD595 nm with Infinite® M200 plate reader (Tecan, Durham, NC). For the CellTiter-Glo Luminescent Cell Viability Assays, the same procedures as crystal violet assays were used except that white/clear bottom 96-well plates were used and cell viability was measured following manufacturer's recommendations. The concentration of candesartan cilexetil and the dose of UV were indicated in figure legends.
To lay the foundation for the methodologies to discover and characterize inhibitors of pol κ, we used a fluorescent reporter strand displacement assay (Figure 1 and ). The library of bioactive small molecules was tested as seven-point dilution series, with the final concentrations ranging from 2.9 nM to 57 µM. The statistical performance of the screening assay as expressed by its Z′ factor remained consistently high with only few deviations, at an average of 0.85 (the maximum Z′ factor possible is 1.0, while values of greater than 0.5 are considered an indication of a highly stable screening assay) (Figure 2A).
Pol κ-catalyzed DNA synthesis displaces TAMRA-labeled reporter strand. This process relieves the quenching effect of BHQ-2, leading to an increase in fluorescence signals.
(A) Excellent Z′ factor of >0.75 was maintained throughout the screen. (B) Concentration-response curves of top hits derived from the screen.
The concentration-response screen paradigm used here  allowed for calculation of an inhibitory potency IC50 value for each positive hit in the primary screen, as well as an evaluation of the shape and other characteristics of the concentration response curve. A total of 60 hits displaying full concentration-response curves and IC50 values of less than 50 µM were identified (the qHTS results are available in PubChem under Assay Identifier 588579, http://pubchem.ncbi.nlm.nih.gov/ and Table S1), and concentration response curves of representative top hits are shown in Figure 2B. The results from these experiments were combined with the radioactive gel-based secondary primer extension assays described below in order to nominate a small subset of hits for detailed characterization.
Candesartan cilexetil, manoalide, and MK-886 inhibit pol κ-catalyzed primer extensions on non-damaged DNA in vitro
In order to confirm the reliability of the qHTS and to serve as a proof-of-principal that this method can be utilized in the further future screenings to identify pol κ inhibitors with potential for drug development, 60 of the hits that were identified through qHTS were analyzed by an orthogonal detection method, consisting of a radioactive gel-based primer extension assays using non-damaged DNA. Initially, the assay was carried out at 80 µM of each compound in order to identify false-positive compounds that were inactive against pol κ, even at this high concentration (Table S1). Using this assay, 3 compounds were shown to have minimal effect on pol κ and thus were not considered in further analyses. Additionally, 5 compounds interfered with the migration of the DNA into the gel and were excluded from further analyses due to potential solubility problems and a lack of availability of these compounds in significant amounts. Thus, a total of 8 compounds were excluded from further analyses. The remaining 52 compounds showed a range of inhibitory activity against pol κ at 80 µM. Based on the compounds' activity in the primer extension assays, the presence of reactive functional group(s) in the compounds, their tendency to appear as actives in a large number of internally-conducted screens (i.e., their promiscuity), and the commercial availability of the compounds to enable further studies, candesartan cilexetil, manoalide, and MK-886 were selected as compounds that would serve as proof-of-principal chemicals for further biochemical and biological assay development. The IC50 values of these compounds were examined by primer extension assays with various concentrations of the compounds, with the results showing that these compounds inhibited pol κ activity in a dose-dependent manner, and the IC50s of candesartan cilexetil, manoalide, and MK-886 on non-damaged DNA were 9.2±0.5 µM, 3.4±0.9 µM, and 13±1.7 µM, respectively (Figure 3 and Figure 4). Despite significant differences between the fluorescence substrate-based HTS method and the radioactive gel-based primer extension assay, IC50s obtained from qHTS and primer extension assays were found to be well-correlated (Figure 4).
Representative data from three independent experiments are shown. The gels shown on the top panel were used to generate dose-response curves shown on the bottom panel.
In primer extension assays, three independent experiments were carried out, and IC50s are shown as average ± standard error.
Candesartan cilexetil, manoalide, and MK-886 inhibit pol κ-catalyzed TLS past acrolein-derived ring-opened reduced form of γ-HOPdG lesions in vitro
The primary role of pol κ is to bypass DNA lesions. Therefore, it was critical to establish the assays to investigate the capability of these compounds to inhibit lesion bypass activity of pol κ. Radioactive gel-based primer extension reactions were carried out using a DNA substrate containing a site-specific acrolein-derived ring-opened reduced form of γ-HOPdG, a lesion that pol κ can efficiently bypass in vitro . As shown in Figure 5, all these compounds were capable of inhibiting pol κ-catalyzed TLS past the lesion in a dose-dependent manner with potencies similar to those shown for the inhibition of pol κ-catalyzed primer extensions on non-damaged DNA. The IC50s obtained from these reactions were 11±1.5 µM for candesartan cilexetil, 5.6±0.9 µM for manoalide, and 14±0.9 µM for MK-886 (Figure 4).
Representative data from three independent experiments are shown. The gels shown on the top panel were used to generate dose-response curves shown on the bottom panel.
Candesartan cilexetil enhances UV-induced cellular toxicity
To assess the potential biological efficacy that would be conferred by inhibiting pol κ, it is necessary to maximize the difference in the cellular endpoint between wild type and pol κ-deficient cells. In order to address this goal, we chose to exploit differential cytotoxicity as an endpoint. Even though the effect of reduction in cellular pol κ on mitomycin C-induced cellular toxicity has been shown, this enhanced cytotoxicity was only slightly less than 2-fold . However, Ziv et al. previously reported that XP-V cells had 3.5- to 5-fold enhanced sensitivity to UV irradiation when pol κ was depleted versus control cells . Thus, to demonstrate that at least a subset of these small molecule inhibitors could exhibit biological efficacy in a cytotoxicity assay, we hypothesized that a pol κ inhibitor should be capable of enhancing the cytotoxic effect of UV exposure in XP-V cells. To test this hypothesis, XP-V cells were treated with candesartan cilexetil or UV alone, or in combination, and assayed for cell viability using a crystal violet staining assay. As shown in Figure 6, the inhibitor potentiated the cytotoxicity of UV at higher UV doses (4.5 and 7.5 J/m2). The assay was also repeated with the CellTiter-Glo Luminescent Cell Viability Assay, that measures ATP content of the cells, and comparable results were obtained (Figure S1). These data suggest that this assay may be a useful tool in the design of a cell-based high throughput screening assay for the identification and characterization of additional compounds and that candesartan cilexetil may inhibit intracellular pol κ. Cell viability was also investigated with manoalide and MK-886. Although both compounds could inhibit pol κ activity in vitro, they failed to enhance UV toxicity in these cells under the conditions investigated (data not shown).
XP30RO cells were treated with 24 µM of candesartan cilexetil alone, UV alone at 1.5 J/m2, 4.5 J/m2, or 7.5 J/m2, or in combination. Percent survival was calculated by normalizing the data using the cell survival of untreated cells. The data were obtained from three independent experiments. Error bars represent standard deviations. I: candesartan cilexetil.
Polymerase selectivity of candesartan cilexetil to inhibit replicative and other TLS polymerase-catalyzed primer extensions on non-damaged DNA in vitro
In order to address the specificity of candesartan cilexetil in inhibiting DNA polymerases, radioactive gel-based primer extension assays were carried out on non-damaged DNA with other TLS polymerases (pol η and pol ι) (Table 1). These data revealed that candesartan cilexetil inhibited both of these polymerases at concentrations comparable to that of pol κ, suggesting that this compound may serve as a general TLS polymerase inhibitor and possibly that it could simply be a general polymerase inhibitor. However, the cellular data using the XP-V cells suggested that this compound is unlikely to be an inhibitor of any of the replicative polymerases, since the cell survival was not altered in the presence of 24 µM candesartan cilexetil. In order to address this assumption, primer extension reactions were carried out with yeast pol δ with several concentrations of candesartan cilexetil, with the maximum concentration being 80 µM. These data revealed that only at 80 µM concentration was there detectable inhibition of DNA synthesis (data not shown) and since all lower concentrations tested showed minimal inhibition, IC50 values could not be determined. These data are consistent with the normal cell growth observed in tissue culture. Thus, we conclude that candesartan cilexetil is not a general polymerase inhibitor, but is not selective toward pol κ.
Candesartan cilexetil does not intercalate into DNA
It is possible that candesartan cilexetil inhibited pol κ activity by blocking the access of pol κ to DNA. Thus, the property of this compound to intercalate into DNA was investigated. As shown in Figure 7, upon mixing of candesartan cilexetil or a control well-known DNA intercalator, ethidium bromide, with double-stranded DNA, the bands shifted upwards in the presence of ethidium bromide, while no difference in DNA migration pattern was observed with candesartan cilexetil compared to control. These results suggest that candesartan cilexetil is unlikely to intercalate into DNA.
Candesartan cilexetil or ethidium bromide was incubated with double-stranded DNA ladder, and the reaction products were run on 1% agarose gel and stained with ethidium bromide. Lane 1: No compound. Lane 2: 0.75 nmol of candesartan cilexetil was incubated with 1.5 nmol DNA. Lane 3: 3 nmol of candesartan cilexetil was incubated with 1.5 nmol DNA. Lane 4: 0.75 nmol of ethidium bromide was incubated with 1.5 nmol DNA. Lane 5: 3 nmol of ethidium bromide was incubated with 1.5 nmol DNA.
Despite the potential significance of targeting pol κ in cancer therapeutics, limited investigations have been reported concerning the discovery of pol κ inhibitors. Although compounds with inhibitory activity against pol κ have been identified, most of these inhibitors were discovered as part of screens of natural products against any DNA polymerase and their therapeutic potential may be limited primarily due to either low potency or poor selectivity. For example, a derivative of a natural product kohamaic acid, (1S*,4aS*,8aS*)-17-(1,4,4a,5,6,7,8,8a-octahydro-2,5,5,8a-tetramethylnaphthalen-1-yl)heptadecanoic acid and a derivative of vitamin K2 and vitamin K3, MK-2, have been shown to inhibit pol κ activity with IC50s of 7.2 µM and 35.3 µM, respectively , . Pol κ activity is also inhibited by C12:0-Acyl juglone and C18:1-Acyl juglone with IC50s of 6.8 µM and 8.1 µM, respectively . Glycyrrhetinic acid is another compound that inhibits pol κ activity (IC50 of 15.8 µM) . Penicilliols A and B are other natural products that inhibit pol κ; however, the activities of these compounds are higher against mouse pol ι than pol κ with an IC50 against pol ι of 19.8 µM and 32.5 µM for penicilliols A and B, respectively . Collectively, these compounds have low potency as well as poor selectivity against pol κ, since they inhibit many other DNA polymerases with similar potency as they inhibit pol κ. Additionally, although penta-1,2,3,4,6-O-galloyl-beta-D-glucose exhibits nanomolar potency against pol κ (IC50 of 30 nM), it is more potent against pol α (IC50 of 13 nM) . Thus, the value of this compound as pol κ inhibitors is lowered by poor selectivity. In contrast to the aforementioned compounds, 3-O-methylfunicone has been determined to exhibit high selectivity against pol κ. However, its low potency (IC50 of 12.5 µM) limits its utilization as a pharmaceutical or as a tool compound to probe pol κ biology . Collectively, these studies emphasize the importance of the identification of pol κ inhibitors with improved potency.
Here we reported the first study to utilize the combination of qHTS and a series of secondary validation assays, including primer extension assays on non-damaged templates, replication bypass assays using site-specifically modified oligodeoxynucleotides, cell survival assays, and DNA intercalation assays for the discovery of small molecule inhibitors of pol κ. A total of 60 compounds identified through qHTS were selected as proof-of-principal chemicals and validated in radioactive primer extension assays with electrophoretic separation. The majority of these hits inhibited pol κ activity at the top concentration tested (80 µM), demonstrating the sensitivity of the qHTS assay to accurately identify positive hits and the reliability of the primer extension assays using non-damaged DNAs to confirm the hits.
After elimination of weak inhibitors, compounds with potentially reactive functionalities or other undesirable chemical features, as well as compounds for which there were no convenient commercial sources, further validation of the remaining three compounds, candesartan cilexetil, manoalide, and MK-886 were conducted by radioactive gel-based primer extension assays. The results revealed that these compounds were capable of inhibiting the ability of pol κ to catalyze synthesis on either a control non-damaged DNA template or a template adducted with the acrolein-derived ring-opened reduced form of γ-HOPdG in a dose-dependent manner with similar potency. Since the predominant role of pol κ is in DNA lesion bypass, these results demonstrated that the primer extension assays using damage-containing DNAs can effectively measure the ability of the compounds to inhibit a biologically relevant activity of pol κ.
In order to assess the ability of these compounds to target intracellular pol κ, cell survival assays were carried out by exposing cells to the combination of pol κ inhibitors and UV. The results showed that candesartan cilexetil could potentiate cellular toxicity induced by UV in XP-V cells. It cannot be ruled out that the cellular effect of candesartan cilexetil may be partly due to its effect on other proteins in addition to pol κ, including pol η and pol ι, since the compound also inhibited the activities of these polymerases in vitro (Table 1); however, our in vitro results clearly show that pol κ is inhibited by this compound. Additionally, it has been shown that the depletion of either pol η or pol ι in XP-V cells did not enhance UV cytotoxicity . Collectively, these observations suggest that pol κ is inhibited by this compound in the cells, and thus validate the usefulness of this cell-based assay in identifying compounds with potential to inhibit intracellular pol κ.
Although manoalide and MK-886 could inhibit pol κ activity in vitro, these compounds were unable to enhance UV-induced toxicity in XP-V cells under the conditions tested. Both manoalide and MK-886 have anti-inflammatory activity; manoalide is well-known as a non-specific phospholipase A2 antagonist –, and MK-886 inhibits leukotriene synthesis by blocking 5-lipoxygenase-activating protein . The reason for the inability of these compounds to potentiate UV cytotoxicity could be due to their significantly lower binding affinity to intracellular pol κ relative to other cellular targets. Alternatively, these compounds may take a long time to enter the cells and bind to pol κ. Moreover, it is possible that only a small fraction of intracellular pol κ is inhibited by these compounds and the remaining pol κ may be sufficient to process UV-induced DNA lesions, resulting in unaltered cellular sensitivity to UV. Given the presence of multiple back-up TLS polymerases, nearly-complete inhibition of the activity of all intracellular pol κ may be essential for cells to present an apparent phenotype. Further understanding of the inability of these compounds to target intracellular pol κ could involve structure-activity relationship analyses. In fact, several structural analogues of these compounds exist such as secomanoalide and luffariellolide for manoalide  and L538,916 for MK-886 , thus enabling the initiation of such studies.
In summary, we presented herein the development of new strategies for the discovery of small molecules that could inhibit pol κ activity both in vitro and in vivo. The identification of chemotypes with established drug properties targeting pol κ validates this qHTS platform, as well as the secondary assays and sets the stage for exploration of significantly larger diverse collections to discover compounds with high potency and specificity towards pol κ and thus could potentially be used as pharmaceuticals. Therefore, these studies would move the research effort one step closer to the development of pol κ-targeted novel combination cancer therapeutics.
Effect of candesartan cilexetil on UV-induced cytotoxicity using CellTiter-Glo Luminescent Cell Viability Assay. XP30RO cells were treated with 24 µM of candesartan cilexetil alone, UV alone at 1.5 J/m2, 4.5 J/m2, or 7.5 J/m2, or in combination. Percent survival was calculated by normalizing the data using the cell survival of untreated cells. I: candesartan cilexetil.
Summary of results of qHTS and radioactive gel-based primer extension assays1. 1In both qHTS and the radioactive gel-based primer extension assays, values of % inhibition that are over 100% and below 0% are presented as 100% and 0%, respectively. ND: not determined.
We wish to thank Dr. Carmelo J. Rizzo (Vanderbilt University, Nashville, TN) for the gift of the oligodeoxynucleotides containing either the control unadducted site or the acrolein-derived ring-opened reduced form of γ-HOPdG, Dr. Peter M. J. Burgers (Washington University, St. Louis, MO) for the gift of yeast pol δ, and Dr. James E. Cleaver (University of California, San Francisco, San Francisco, CA) for the gift of XP30RO cell lines.
Conceived and designed the experiments: KY DD RLE ME AJ AS RSL. Performed the experiments: KY DD RLE. Analyzed the data: KY DD AJ DJM AS RSL. Contributed reagents/materials/analysis tools: RLE ME. Wrote the paper: KY AS RSL.
- 1. Goodman MF (2002) Error-prone repair DNA polymerases in prokaryotes and eukaryotes. Annu Rev Biochem 71: 17–50.
- 2. Lange SS, Takata K, Wood RD (2011) DNA polymerases and cancer. Nat Rev Cancer 11: 96–110.
- 3. Waters LS, Minesinger BK, Wiltrout ME, D'Souza S, Woodruff RV, et al. (2009) Eukaryotic translesion polymerases and their roles and regulation in DNA damage tolerance. Microbiol Mol Biol Rev 73: 134–154.
- 4. Guo C, Kosarek-Stancel JN, Tang TS, Friedberg EC (2009) Y-family DNA polymerases in mammalian cells. Cell Mol Life Sci 66: 2363–2381.
- 5. Minko IG, Kozekov ID, Harris TM, Rizzo CJ, Lloyd RS, et al. (2009) Chemistry and biology of DNA containing 1,N2-deoxyguanosine adducts of the α,β-unsaturated aldehydes acrolein, crotonaldehyde, and 4-hydroxynonenal. Chem Res Toxicol 22: 759–778.
- 6. Yamanaka K, Lloyd RS (2012) Functions of Translesion DNA Polymerases: Implications for Cancer Risk and Opportunities as Therapeutic Targets. In: Madhusudan S, Wilson DM, 3rd, editors. DNA Repair and Cancer: Bench to Clinic. Enfield, NH: Science Publishers. In press.
- 7. Zhang Y, Yuan F, Wu X, Wang M, Rechkoblit O, et al. (2000) Error-free and error-prone lesion bypass by human DNA polymerase κ in vitro. Nucleic Acids Res 28: 4138–4146.
- 8. Rechkoblit O, Zhang Y, Guo D, Wang Z, Amin S, et al. (2002) trans-Lesion synthesis past bulky benzo[a]pyrene diol epoxide N2-dG and N6-dA lesions catalyzed by DNA bypass polymerases. J Biol Chem 277: 30488–30494.
- 9. Zhang Y, Wu X, Guo D, Rechkoblit O, Geacintov NE, et al. (2002) Two-step error-prone bypass of the (+)- and (−)-trans-anti-BPDE-N2-dG adducts by human DNA polymerases η and κ. Mutat Res 510: 23–35.
- 10. Sassa A, Niimi N, Fujimoto H, Katafuchi A, Gruz P, et al. (2011) Phenylalanine 171 is a molecular brake for translesion synthesis across benzo[a]pyrene-guanine adducts by human DNA polymerase kappa. Mutat Res 718: 10–17.
- 11. Minko IG, Yamanaka K, Kozekov ID, Kozekova A, Indiani C, et al. (2008) Replication bypass of the acrolein-mediated deoxyguanine DNA-peptide cross-links by DNA polymerases of the DinB family. Chem Res Toxicol 21: 1983–1990.
- 12. Minko IG, Harbut MB, Kozekov ID, Kozekova A, Jakobs PM, et al. (2008) Role for DNA polymerase κ in the processing of N2-N2-guanine interstrand cross-links. J Biol Chem 283: 17075–17082.
- 13. Wolfle WT, Johnson RE, Minko IG, Lloyd RS, Prakash S, et al. (2005) Human DNA polymerase ι promotes replication through a ring-closed minor-groove adduct that adopts a syn conformation in DNA. Mol Cell Biol 25: 8748–8754.
- 14. Washington MT, Minko IG, Johnson RE, Wolfle WT, Harris TM, et al. (2004) Efficient and error-free replication past a minor-groove DNA adduct by the sequential action of human DNA polymerases ι and κ. Mol Cell Biol 24: 5687–5693.
- 15. Ogi T, Lehmann AR (2006) The Y-family DNA polymerase κ (pol κ) functions in mammalian nucleotide-excision repair. Nat Cell Biol 8: 640–642.
- 16. Ziv O, Geacintov N, Nakajima S, Yasui A, Livneh Z (2009) DNA polymerase ξ cooperates with polymerases κ and ι in translesion DNA synthesis across pyrimidine photodimers in cells from XPV patients. Proc Natl Acad Sci U S A 106: 11552–11557.
- 17. Bi X, Slater DM, Ohmori H, Vaziri C (2005) DNA polymerase κ is specifically required for recovery from the benzo[a]pyrene-dihydrodiol epoxide (BPDE)-induced S-phase checkpoint. J Biol Chem 280: 22343–22355.
- 18. Ho TV, Scharer OD (2010) Translesion DNA synthesis polymerases in DNA interstrand crosslink repair. Environ Mol Mutagen 51: 552–566.
- 19. Raschle M, Knipscheer P, Enoiu M, Angelov T, Sun J, et al. (2008) Mechanism of replication-coupled DNA interstrand crosslink repair. Cell 134: 969–980.
- 20. Noll DM, Mason TM, Miller PS (2006) Formation and repair of interstrand cross-links in DNA. Chem Rev 106: 277–301.
- 21. Lehoczky P, McHugh PJ, Chovanec M (2007) DNA interstrand cross-link repair in Saccharomyces cerevisiae. FEMS Microbiol Rev 31: 109–133.
- 22. Mamelak AN (2005) Locoregional therapies for glioma. Oncology 19: 1803–1810.
- 23. Wang H, Wu W, Wang HW, Wang S, Chen Y, et al. (2010) Analysis of specialized DNA polymerases expression in human gliomas: association with prognostic significance. Neuro Oncol 12: 679–686.
- 24. Mizushina Y, Motoshima H, Yamaguchi Y, Takeuchi T, Hirano K, et al. (2009) 3-O-methylfunicone, a selective inhibitor of mammalian Y-family DNA polymerases from an Australian sea salt fungal strain. Mar Drugs 7: 624–639.
- 25. Irimia A, Eoff RL, Guengerich FP, Egli M (2009) Structural and functional elucidation of the mechanism promoting error-prone synthesis by human DNA polymerase κ opposite the 7,8-dihydro-8-oxo-2′-deoxyguanosine adduct. J Biol Chem 284: 22467–22480.
- 26. Cleaver JE, Afzal V, Feeney L, McDowell M, Sadinski W, et al. (1999) Increased ultraviolet sensitivity and chromosomal instability related to P53 function in the xeroderma pigmentosum variant. Cancer Res 59: 1102–1108.
- 27. Dorjsuren D, Wilson DM 3rd, Beard WA, McDonald JP, Austin CP, et al. (2009) A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases. Nucleic Acids Res 37: e128.
- 28. Huang R, Southall N, Wang Y, Yasgar A, Shinn P, et al. (2011) The NCGC pharmaceutical collection: a comprehensive resource of clinically approved drugs enabling repurposing and chemical genomics. Sci Transl Med 3: 80ps16.
- 29. Inglese J, Auld DS, Jadhav A, Johnson RL, Simeonov A, et al. (2006) Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical libraries. Proc Natl Acad Sci U S A 103: 11473–11478.
- 30. Mizushina Y, Maeda J, Irino Y, Nishida M, Nishiumi S, et al. (2011) Effects of Intermediates between Vitamins K2 and K3 on Mammalian DNA Polymerase Inhibition and Anti-Inflammatory Activity. Int J Mol Sci 12: 1115–1132.
- 31. Mizushina Y, Manita D, Takeuchi T, Sugawara F, Kumamoto-Yonezawa Y, et al. (2009) The inhibitory action of kohamaic acid A derivatives on mammalian DNA polymerase β. Molecules 14: 102–121.
- 32. Maruo S, Kuriyama I, Kuramochi K, Tsubaki K, Yoshida H, et al. (2011) Inhibitory effect of novel 5-O-acyl juglones on mammalian DNA polymerase activity, cancer cell growth and inflammatory response. Bioorg Med Chem 19: 5803–5812.
- 33. Ishida T, Mizushina Y, Yagi S, Irino Y, Nishiumi S, et al. (2012) Inhibitory effects of glycyrrhetinic Acid on DNA polymerase and inflammatory activities. Evid Based Complement Alternat Med 2012: 650514.
- 34. Kimura T, Takeuchi T, Kumamoto-Yonezawa Y, Ohashi E, Ohmori H, et al. (2009) Penicilliols A and B, novel inhibitors specific to mammalian Y-family DNA polymerases. Bioorg Med Chem 17: 1811–1816.
- 35. Mizushina Y, Zhang J, Pugliese A, Kim SH, Lu J (2010) Anti-cancer gallotannin penta-O-galloyl-beta-D-glucose is a nanomolar inhibitor of select mammalian DNA polymerases. Biochem Pharmacol 80: 1125–1132.
- 36. Folmer F, Jaspars M, Schumacher M, Dicato M, Diederich M (2010) Marine natural products targeting phospholipases A2. Biochem Pharmacol 80: 1793–1800.
- 37. Ebada SS, Lin W, Proksch P (2010) Bioactive sesterterpenes and triterpenes from marine sponges: occurrence and pharmacological significance. Mar Drugs 8: 313–346.
- 38. Nakao Y, Fusetani N (2007) Enzyme inhibitors from marine invertebrates. J Nat Prod 70: 689–710.
- 39. Evans JF, Ferguson AD, Mosley RT, Hutchinson JH (2008) What's all the FLAP about?: 5-lipoxygenase-activating protein inhibitors for inflammatory diseases. Trends Pharmacol Sci 29: 72–78.
- 40. Smirnov SV, Knock GA, Aaronson PI (1998) Effects of the 5-lipoxygenase activating protein inhibitor MK886 on voltage-gated and Ca2+-activated K+ currents in rat arterial myocytes. Br J Pharmacol 124: 572–578.