Histone deacetylase (HDAC) inhibitors have received considerable attention as potential therapeutics for a variety of cancers and neurological disorders. Recent publications on a class of pimelic diphenylamide HDAC inhibitors have highlighted their promise in the treatment of the neurodegenerative diseases Friedreich’s ataxia and Huntington’s disease, based on efficacy in cell and mouse models. These studies’ authors have proposed that the unique action of these compounds compared to hydroxamic acid-based HDAC inhibitors results from their unusual slow-on/slow-off kinetics of binding, preferentially to HDAC3, resulting in a distinctive pharmacological profile and reduced toxicity. Here, we evaluate the HDAC subtype selectivity, cellular activity, absorption, distribution, metabolism and excretion (ADME) properties, as well as the central pharmacodynamic profile of one such compound, HDACi 4b, previously described to show efficacy in vivo in the R6/2 mouse model of Huntington’s disease. Based on our data reported here, we conclude that while the in vitro selectivity and binding mode are largely in agreement with previous reports, the physicochemical properties, metabolic and p-glycoprotein (Pgp) substrate liability of HDACi 4b render this compound suboptimal to investigate central Class I HDAC inhibition in vivo in mouse per oral administration. A drug administration regimen using HDACi 4b dissolved in drinking water was used in the previous proof of concept study, casting doubt on the validation of CNS HDAC3 inhibition as a target for the treatment of Huntington’s disease. We highlight physicochemical stability and metabolic issues with 4b that are likely intrinsic liabilities of the benzamide chemotype in general.
Citation: Beconi M, Aziz O, Matthews K, Moumné L, O’Connell C, et al. (2012) Oral Administration of the Pimelic Diphenylamide HDAC Inhibitor HDACi 4b Is Unsuitable for Chronic Inhibition of HDAC Activity in the CNS In Vivo. PLoS ONE 7(9): e44498. doi:10.1371/journal.pone.0044498
Editor: Yoshitaka Nagai, National Center of Neurology and Psychiatry, Japan
Received: December 16, 2011; Accepted: August 7, 2012; Published: September 4, 2012
Copyright: © Beconi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: CHDI Foundation is a not-for-profit biomedical research organization exclusively dedicated to discovering and developing therapeutics that slow the progression of Huntington's disease. This work was funded and conducted through fee-for-service contract research on behalf of CHDI Foundation, with the exception of the pharmacodynamic evaluation of 4b that was conducted by King's College London investigators 4, in collaboration with and funded by CHDI Foundation.
Competing interests: The authors have declared that no competing interests exist. CHDI Foundation is a not-for-profit biomedical research organization exclusively dedicated to discovering and developing therapeutics that slow the progression of Huntington’s disease. Evotec (UK) and BioFocus (UK) conducted the research through a fee-for-service agreement for CHDI Foundation. This declaration does not alter the authors’ adherence to all PLoS ONE policies on sharing data and materials.
Pimelic diphenylamide HDAC inhibitors have received renewed attention in recent years due to the efficacy of compounds from this series in the amelioration of phenotypes in Friedreich’s ataxia (FRDA) and Huntington’s disease (HD) cell and mouse models .
Friedreich’s Ataxia Therapy with HDAC Inhibitors
FRDA is the result of a GAA·TTC triplet hyper-expansion in an intron of the frataxin (FXN) gene that leads to transcriptional silencing. FXN is an essential mitochondrial protein and the resultant FXN insufficiency results in progressive spinocerebellar neurodegeneration and cardiomyopathy, leading to a progressive lack of motor coordination, incapacity and eventually death, usually in early adulthood –. In transformed lymphoid cell lines derived from an FRDA patient, histones H3 and H4 associated with the FXN gene are hypo-acetylated with a concomitant increase in trimethylated H3K9 . These findings imply a repressed heterochromatin state and suggest that HDAC inhibitors capable of restoring acetylation to histones may have therapeutic potential.
For FRDA, the effects on both H3 and H4 acetylation and FXN mRNA levels were assessed in cellular models using a variety of hydroxamic acid-based HDAC inhibitors, including valproic acid, TSA, SAHA and suberoyl bishydroxamic acid. These studies gave variable results, confounded by the cellular toxicity of these compounds . However, the pimelic diphenylamide HDAC inhibitor BML-210 was reported to increase FXN mRNA without cytotoxicity at the concentration tested. Further, application to cells of an analog of BML-210, HDACi 4b, resulted in a 2.5-fold enhancement of FXN mRNA (at 5 µM), acetylation of H3K14, H4K5 and H4K12 in the chromatin region immediately upstream of the GAA repeats, and a 3.5-fold increase in FXN protein levels (at 2.5 µM) . A subsequent short pharmacodynamic study in a FRDA mouse model showed that a close analogue of HDACi 4b, the tolyl derivative compound 106, corrected the FXN deficiency . These mice carry a homozygous (GAA)230 expansion in the first intron of the mouse FXN gene (KI/KI mice) . Biochemical analysis revealed that these mice carry the same heterochromatin marks, close to the GAA repeat, as those detected in patient cell lines and have mildly but significantly reduced FXN mRNA and protein levels; however, they show no overt phenotype. Compound 106 given at 150 mg/kg subcutaneously once daily for 3 days increased global brain tissue histone acetylation as well as histone acetylation close to the GAA repeat and restored FXN levels in the nervous system and heart. Reversion of other differentially expressed genes towards wild type levels was also observed. Compound 106 showed no apparent toxicity in this study.
Recently, the long-term benefit of chronic subcutaneous administration of three pimelic o-aminobenzamide inhibitors (compounds 106, 136 and 109) were assessed in another mouse model of FRDA. This mouse model (YG8R) contains the human FXN gene with expanded GAA repeats in a mouse FXN null background , . These mice show an approximate 30% reduction in FXN protein levels, mildly impaired motor coordination in females, reduced aconitase enzyme activity and DRG neuronal pathology, as well as a modest non-significant reduction in weight. However, YG8R mice show no evidence of hypoacetylation of H3 or H4 histones relative to WT or a reduction in FXN mRNA compared to WT .
The HDAC inhibitors were administered at 150 mg/kg (106), 50 mg/kg (136) and 100 mg/kg (109) by 3 (106) or 5 (136 and 109) subcutaneous injections per week to YG8R and WT mice for 4.5 to 5 months; the rationale for the different dosing and frequency were not given, and to our knowledge, no ADME data has been presented on this series. Although generally well tolerated, the inhibitors gave variable results. The authors concluded that prolonged treatment with any of the three HDAC inhibitors 106, 136 and 109 ameliorated FRDA disease-like pathology to some extent, and speculated that the apparent discrepancy in outcome with the three inhibitors could be due to differences in their potency, specificity, tissue distribution, and brain penetrance, as well as differences in dose levels and dose frequency resulting in sub maximal exposure .
Huntington’s Disease Therapy with HDAC Inhibitors
HD is a lethal autosomal dominant neurodegenerative disease caused by expansion of a stretch of CAG-encoded glutamines near the N-terminus of huntingtin (HTT) , a protein whose mutant form accumulates as nuclear and cytoplasmic inclusions in the brain of HD patients . The disease is a progressive disorder with severe psychiatric, cognitive, and motor impairments. Mutant HTT (mHTT) confers a particular vulnerability to the medium spiny neurons of the corpus striatum, as well as subsets of cortical neurons in the motor, frontal, and occipital cortices, and in other brain regions such as the hypothalamus . Age of onset in humans is inversely correlated to the size of the CAG expansion, with expansions >39 CAGs in the HTT gene resulting in complete penetrance of the disease. The cellular and biological pathways affected by mHTT are widespread, including transcriptional dysregulation, disruption of energy homeostasis, impairment of protein turnover by the ubiquitin-proteasome system (UPS) and the autophagy-lysosomal system, and impairment of synaptic transmission and plasticity. HDAC inhibition has been proposed as a therapeutic strategy for HD (reviewed in –). Indeed, broad-spectrum HDAC inhibitors partially rectify the transcriptional dysregulation in HD cell and animal models –, enhance the degradation of mHTT by altering the acetylation state of key residues within the protein –, and improve cognition through enhancement of learning and memory processes , .
Thomas et al showed that HDACi 4b has a therapeutic effect in the R6/2 HD mouse model . The R6/2 strain used in this study expresses the exon 1 HTT protein with an expanded polyglutamine region of ~300 repeats (R6/2300Q), and manifests a delayed phenotype compared to the better characterised R6/2 model that has a shorter polyglutamine expansion –. The R6/2300Q mice exhibit significant deficits in motor behaviour by 12 weeks of age, striatal atrophy, and survive 6 to 7 months. A short pharmacodynamic study (once daily subcutaneous treatment with 150 mg/kg 4b for 3 days) successfully ameliorated gene expression abnormalities in these mice and showed increased histone H3 acetylation in association with selected down-regulated genes. In a chronic efficacy study, 4b was complexed to 2-hydroxypropyl-β-cyclodextrin and diluted in drinking water (estimated dosage of 150 mg/kg/day) and given to mice from 4 months of age. However, the expected differences in oral versus parenteral administration were not addressed in the Thomas et al. study . While this precludes direct correlation between the pharmacodynamic studies and the results of the efficacy trial, these mice showed improved motor performance and overall appearance and an amelioration of body weight loss. Gross brain weight and striatal volume were also improved on termination of the study at 6 months of age.
The successful use of 4b in treating R6/2 mice loosely correlates with an earlier report, in which the hydroxamic acid HDAC inhibitor SAHA was administered in drinking water to R6/2 mice that harbour the smaller polyglutamine repeat (~200 Q) and exhibit a more aggressive phenotype . These animals also showed significant improvement in motor dysfunction as assessed by rotarod performance and grip strength, but this improvement was offset by the failure of both wild type and R6/2 mice to gain weight at the maximum tolerated dose (0.67 g/L in drinking water), suggestive of a narrow therapeutic window.
Pharmacology of Pimelic Diphenylamide-based HDAC Inhibitors
Subsequent reports provided an intriguing explanation for the efficacy and well-tolerated effects of the pimelic diphenylamide-based inhibitors. A common feature of these HDAC inhibitors (also known as ortho-N-acyl-phenylene diamines or benzamides) is an acylated ortho-phenylene diamine unit, which is thought to interact with the zinc ion of HDACs. Compounds from this series are selective for HDAC1, HDAC2, and HDAC3 over other HDAC isoforms, with no activity reported against HDAC Class IIa enzymes and only weak activity reported against HDAC8 , . Furthermore, they appear to bind to the catalytic site of these HDACs via a unique binding mode not shared with other hydroxamic acid based inhibitors; a time-dependent increase in affinity with an extremely slow off rate has been observed –.
These exciting findings plus the report of in vivo efficacy and a neuroprotective profile in the R6/2 HD model prompted us to synthesize and evaluate 4b to independently validate this finding in the more widely used lower CAG repeat length R6/2 model –, and also potentially in other HD rodent models. We had two objectives: to further validate the finding that preferential central HDAC3 inhibition is of therapeutic relevance in R6/2 and other HD models, and to assess the suitability of 4b or structurally-related compounds for translation into clinical trials. We therefore evaluated the pharmacology, ADME, and pharmacokinetic properties of 4b in mice, adhering as closely as possible to the original design for the in vivo work .
Our in vitro biochemical selectivity profiling is largely in agreement with the previous reports. However, we identified a physicochemical instability of 4b that constitutes a potential liability of all published compounds containing an acyl phenylenediamine ‘warhead’: cyclisation of this zinc-binding moiety to an inactive benzimidazole product was observed, which was especially efficient under acidic conditions such as during the solubilisation process of acidic salts of 4b. We suspect that similar solubilisation conditions may have been used in the previous study as part of the oral formulation preparation procedures . Importantly, our in vitro and in vivo ADME evaluation showed high metabolic turnover of 4b and very low brain penetration due to high systemic clearance and efficient transport out of the brain since 4b acts as a Pgp substrate.
In summary, our results are not in agreement with the original conclusions of the Thomas et al study , which suggested that the beneficial improvement in the R6/2 HD mouse model observed with 4b was due to central HDAC (and presumably, based on the later publications, HDAC3) inhibition. Our ADME results led us to the conclusion that further investigation of 4b in in vivo efficacy studies would not be informative.
Biochemical and Cellular HDAC Inhibition by 4b
To assess the selectivity and potency of 4b prepared as the free base and dissolved as a stock solution in DMSO, we profiled inhibition of deacetylase activity against a comprehensive panel of purified human recombinant HDAC enzymes, representative of the three main classes: Class I (HDAC1, HDAC2, HDAC3, HDAC8), Class IIa (HDAC4, HDAC5, HDAC7, HDAC9), and Class IIb (HDAC6). Using our standard screening format (see methods), 4b showed a modest ~6-fold selectivity within the Class I enzymes for HDAC3, with IC50 values of 1.51 µM (HDAC1), 2.23 µM (HDAC2) and 254 nM (HDAC3), respectively (Fig. 1A). No activity was seen against HDAC8 or the Class IIa/b enzymes HDAC 4, 5, 6, 7, and 9 enzymes up to 50 µM 4b (Fig. 1B). This is in reasonable agreement with a previous study with the very closely structurally related analogue of 4b (the tolyl derivative Compound 106).
(A) % inhibition of human recombinant Class I enzymes HDAC1 (red), HDAC2 (green), HDAC3 (black) and HDAC8 (blue) by 4b. (B) No inhibition of ClassIIa/b enzymes by 4b; HDAC 4(green), HDAC5 (red), HDAC7 (purple), HDAC9 (orange), HDAC6 (brown). (C) Time-dependence of human recombinant HDAC3 inhibition by varying preincubation time of 4b with enzyme (as shown). (D) Cellular inhibition of endogenous Class I HDACs/HDAC6 using Boc_Lys_Ac (black traces) or Class IIa/HDAC8 HDACs using Boc_Lys_TFA substrate (red traces) by 4b (closed circles) or reference compounds SAHA and Compound 26. (E) Time- dependence of cellular Class I HDAC inhibition by varying preincubation of 4b with cells (as shown). (F) Plot of IC50 values versus compound-cell preincubation time for SAHA (green) and 4b (black).
As described, the length of compound-enzyme incubation has been reported to be critical for this class of HDAC inhibitors, where a unique time-dependent increase in affinity and subsequent potency has been observed –. To further investigate any ‘time-dependent’ increase in potency, we pretreated recombinant HDAC3 with compound for various incubation times ranging from 0 to 120 min prior to addition of the Boc_Lys_Ac substrate for a further 60 min. To better mimic physiological conditions, our experiments were performed at 37°C as opposed to room temperature as reported in previous studies . As can be observed in Fig. 1C, a leftward shift of the inhibition curve with increasing incubation time was observed, with a shift in the IC50 from 0.65 µM with 0 min pre-incubation, to 80 nM following a 2 h pre-incubation. To confirm that this shift was independent of the time of 4b in aqueous solution, 4b was applied immediately to the enzyme-substrate mix both after preparing in aqueous buffer, and 120 min after preparation. In this instance, the IC50 remained relatively constant (0.97 and 0.65 µM respectively, confirming that pre-incubation with enzyme was necessary for the increased potency (data not shown). These findings corroborate those of Chou et al, (2008)  using analogue 106. In addition to examining biochemical potency against the recombinant enzymes, we also assessed the cellular potency of 4b for Class I/IIb or Class IIa/HDAC8 inhibition, by utilizing the enzyme selectivity of the two cell permeable substrates used: Boc_Lys_Ac and Boc_Lys_TFA, respectively. In agreement with the biochemical profiles, incubation of Jurkat E6.1 cells with 4b showed no inhibition when the Class IIa/HDAC8 substrate was used (up to 50 µM 4b), but gave a relatively weak cellular IC50 of 5.3 µM under our standard protocol of a total enzyme-compound incubation time of 5 h when Boc_Lys_Ac was used as the substrate to query Class I inhibition (Fig. 1D).
To extend these findings, we examined whether a time-dependent increase in potency could be observed with 4b in a cellular context, and thus we repeated this experiment with an extended time course of preincubation of between 0 to 24 h. In this assay, we also observed a shift in the cellular IC50 of 4b with increasing incubation time prior to substrate addition, with a measured cellular IC50 of 16 µM with no pre-incubation, to 1.8 µM after maximal 24 h incubation (Fig. 1E and F). As a control, we also evaluated the effect of SAHA, a Class I/IIb selective hydroxamic acid HDAC inhibitor that does not share these binding characteristics. In contrast to 4b, no time-dependent shift in IC50 was observed for SAHA, and the IC50 remained constant at 220 and 240 nM, respectively (Fig. 1F). As a secondary control, freshly prepared 4b compound dilution was compared to dilutions made 21 h previously in DMSO or aqueous buffer. When compounds were not pre-incubated with cells prior to substrate addition, no shift in IC50 occurred (IC50 from freshly prepared compound = 16 µM, and from compound prepared 21 h previously = 15 µM; data not shown).
In conclusion, our data are largely in agreement with previous literature. Here, we extend these by providing a full in vitro functional selectivity profile of 4b, as a selective Class I inhibitor, with an ~6- to 9 fold selectivity for HDAC3 over HDAC1 and HDAC2, respectively, and slow apparent binding kinetics to HDAC3, resulting in a maximal biochemical IC50 for HDAC3 of 80 nM following 3 h incubation. The increase in affinity with prolonged incubation time to HDAC1 or HDAC2 was not studied, but it is likely that this is also a feature of these enzymes , although possibly less pronounced . Importantly, we provide a well characterised cellular profile, which greatly aids an understanding of adequate concentrations needed to be attained in plasma or brain to effectively inhibit the target in a native cell environment. In this study, a maximal cellular IC50 for ‘Class I’ HDAC inhibition of 1.8 µM after 24 h incubation was achieved.
Despite the relatively weak cellular IC50 returned, the verification of this profile encouraged us to continue to pursue this compound to assess its potential for in vivo proof of concept studies in rodent models of Huntington’s disease, and to attempt to replicate the earlier findings of Thomas et al (2008) .
Assessment of Potential Off-target Activity of 4b
In addition to the HDAC profiling performed, we also queried 4b for any apparent potential off-target effects, which could complicate any finding of efficacy or adverse events in vivo due to ‘polypharmacy’. This was accomplished by screening against a ‘diversity panel’ of 72 binding and 29 enzyme assays, comprised of roughly equal numbers of selective central and peripheral therapeutically relevant targets (Cerep Diversity Profile). This panel included representative targets from diverse enzyme families, G protein coupled receptors, steroid nuclear receptors, voltage- and ligand-gated ion channels. While not totally inclusive, it is a useful tool for discovery compounds to assess their viability as proof of concept molecules to specifically query a particular target. 4b was initially screened at 10 µM in duplicate. The full set of results is provided as Table S1. 4b showed a ‘clean’ profile, with only 1 target, the CCK1 receptor showing 72% displacement of ligand binding on addition of 10 µM 4b. A follow up concentration-effect curve gave a Ki of 8.6 µM for 4b against CCK1 binding (Fig. S1). This clean in vitro profile was considered acceptable to proceed to ADME and pharmacokinetic studies.
Solubility and Physicochemical Stability of Free Base and Salt Forms of 4b
Compound 4b prepared as the free base proved to be highly insoluble in water at neutral pH. We next attempted to formulate 4b according to the given literature procedure , which had been adapted from a similar procedure previously used in the preparation of SAHA . This involved the complexing of 4b to 2-hydroxypropyl-β-cyclodextrin in a 1:5 molar ratio to aid aqueous solubility. Under the prescribed formulation procedure targeting a 1 mg/mL solution, we found that 4b as a free base did not dissolve under these conditions even on heating, but instead formed a suspension. LC-MS analysis of this suspension on day 1 and after 7 days indicated no degradation of 4b when prepared either with or without heating. Filtering the suspension and quantifying the filtrate showed that only 0.1 mg/ml of 4b had dissolved.
Thomas and colleagues  reported that a solution of 4b at 1 mg/mL was previously obtained using this procedure. A further examination of the reference cited for 4b preparation by this group showed that the 4b used was purified by preparative HPLC with trifluoracetate (TFA) in the eluent  suggesting that the authors had most likely isolated and used the TFA salt in their studies. We therefore prepared both TFA and hydrochloride (HCl) salts of 4b to establish whether they could offer improved solubility. Neither salt form of 4b was particularly soluble in water (<1 mg/ml). When prepared according to the prescribed formulation procedures, the TFA salt of 4b did not dissolve, even after heating. Analysis of the filtrate after filtering the suspension prepared without heating showed that only 0.3 mg/mL had fully dissolved. A study of the stability of the TFA salt also raised another more worrying issue in the use of 2-hydroxypropyl-β-cyclodextrin–complexed 4b salts for potential in vivo studies. We observed that both salt forms of 4b cyclised slowly when left in solution (eg. aqueous acetonitrile) to a benzimidazole product at ambient temperature and more rapidly when heated (see Fig. 2 schema). The 4b. TFA cyclodextrin suspension targeted at 1 mg/ml with heating gave an initial (day 1) measured value of 0.75 mg/ml total 4b, with only 0.27 mg/mL solubilised as quantified in the sample after filtration. The lower than expected initial quantification of 4b was most likely due to the TFA salt rapidly degrading upon heating. Indeed, we always found the presence of the cyclised benzimidazole conversion product, named throughout as product C1, in the salt formulations even at t = 0 after preparation. Furthermore, the suspension was not stable upon standing in the dark with gentle shaking at room temperature, with a further 28% decrease observed in parent (4b) concentration on day 7 analysis, compared to day 1. Importantly, we anticipate that the conversion of benzamide 4b to the benzimidazole C1 is not reversible under physiological conditions and we have no experimental evidence indicating that C1 could be converted back to 4b.
LC-MS identification of 4b (red) and C1 (green) metabolite products after incubation in mouse plasma and mouse hepatic microsomes. Metabolites of 4b included amide hydrolysis products M1 and M2, and the NADPH-dependent hydroxylated metabolites M4, M5 and M6. Metabolites of C1 included the amide hydrolysis product M3, the NADPH-dependent mono-hydroxylated products M7 and M8 and di-hydroxylated products M9 and M10. Boxed metabolites were present in both plasma and hepatic microsomal incubations, while the hydroxylated products were only present in hepatic microsomal incubations.
In order to assess the benzimidazole conversion product that we detected, we prepared a pure sample. This purified product C1 was readily soluble in aqueous media. However, profiling against the biochemical and cellular HDAC assays determined that C1 retained no activity against any HDACs, or in the cellular Boc_Lys_Ac assay, at concentrations up to 50 µM, as would be expected due to the cyclisation of the ‘warhead’ benzamide of the compound, which is needed to chelate to the zinc moiety in the HDAC catalytic site. It did however retain the ability to displace CCK1 agonist radioligand binding in the Cerep assay (IC50 = 6.1 µM, Fig. S1). In addition, a further profiling of C1 at 10 µM against the Diversity Profile (Table S1) also showed significant additional displacement of antagonist radioligand binding for the I2 receptor (73% displacement).
In conclusion, the insolubility and instability of 4b. TFA in aqueous solutions, which is exacerbated by heating as occurred during the formulation procedure previously used, casts grave doubt on the final 4b/C1 ratio administered to mice during the chronic oral drinking water study previously performed in the Thomas et al study , where the formulation in drinking water bottles were also reported to be changed weekly. Indeed, achieving a 1 mg/mL solution at all with 4b in this formulation would indicate that the resultant dosing solution may have been converted to predominantly the ‘inactive’ and readily soluble C1 product.
In vitro ADME Properties of 4b and C1
In order to understand the predicted in vivo stability and potential metabolism routes of 4b, either 4b or C1 were incubated with mouse plasma and mouse hepatic microsomes and putative metabolites identified using mass spectrometry (LC-MS). Mass chromatograms were generated for ions seen to increase in the compound incubates relative to controls, and also for Phase I (oxidative) metabolic and hydrolytic cleavage products considered likely based on the compound structures. Where chromatographic peaks of greater intensity were present in the incubated samples, daughter (fragmentation) spectra were obtained and structures proposed for putative metabolites based on the fragmentation pattern when compared to those of parent compounds. A total of 10 products were identified (Fig. 2). Metabolites of 4b included the two amide hydrolysis products, M1 and M2, present in plasma and hepatic microsomal incubations, and the NADPH-dependent mono-hydroxylated metabolites M4, M5 and M6, present in hepatic microsomal incubations. C1 corresponded to the acid-catalyzed cyclization product (6-(1-H-benzo[d]imidazol-2-yl)-N-phenylhexanamide) of 4b, and was not a product of enzymatic metabolism. Metabolites of C1 included the product of amide hydrolysis M3, present in plasma and hepatic microsomal incubations and the NADPH-dependent mono-hydroxylated products M7 and M8 and di-hydroxylated products M9 and M10, present in hepatic microsomal incubations. The structures of metabolites M1, M2 and M3 were confirmed by comparing their chromatographic retention time and MS/MS spectra to that of the corresponding synthetic standards. All other structural assignments are proposed by MS/MS.
In vitro metabolism and permeability.
4b was very unstable in vitro in plasma and in liver microsomal incubations. Following incubations of 4b (5 µM, duplicates) in fresh mouse plasma, the half-life was 1.9 h and complete conversion to the amide hydrolysis products (M1 and M2), was achieved within 6 h (Fig. 3A). The main hydrolysis product in plasma was M1 which accounted for 78% of the metabolism of parent, followed by M2 which accounted for 10% of the metabolism observed. 4b was stable in incubations in serum albumin, confirming the metabolic nature of the observed products. C1 was also unstable in vitro in plasma (Fig. 3B). Following incubations of C1 (5 µM, duplicates) in fresh mouse plasma, the half-life was 3.6 h. One amide hydrolysis product, M3, was confirmed by metabolite identification studies and accounted for 80% of metabolism of parent (Fig. 3B).
(A) Time course of metabolism of 4b (5 µM), and generation of metabolites M1 and M2 in mouse plasma. (B) Time course of metabolism of C1 (3 µM), and generation of metabolites M3 in mouse plasma. (C and D) Time course of metabolism of 4b (1 µM), and generation of metabolites M1, M2 and M4 in mouse hepatic microsomes, in presence (C) and absence (D) of NADPH. The dashed black line indicates the sum of 4b and metabolites measured at each time point.
The in vitro half-life of 4b in mouse hepatic microsomes (1 µM, duplicates) in the presence of the cofactor NADPH was approximately 20 min (Fig. 3C), resulting in a very high predicted in vivo plasma clearance of 2.6 L/h/kg, or approximately 87% of liver plasma flow. Near complete metabolism of 4b was seen after 40 min incubation with mouse hepatic microsomes (16% of parent remained), with the amide hydrolysis products, M1 and M2 accounting for 76% of parent converted. Several hydroxylated metabolic products were identified in metabolite identification studies. Of those synthesised and monitored, para hydroxylation on the phenyl amine accounted for a further 3% of the metabolism observed in liver microsomes (M4). The rank order in abundance of metabolites was similar to that observed in plasma, the most significant metabolite in hepatic microsomes was M1 which accounted for 42% of the metabolism of parent, while M2 accounted for 34% of the metabolism. Since the amide hydrolysis products were observed in the absence of NADPH (Fig. 3D), their formation was attributed to non-CYP-mediated metabolism or amidases present in hepatic microsomes. Concentrations of the benzimidazole product C1 were below the limit of quantification in all incubations with 4b (0.1 µM), confirming that the benzimidazole product is not a product of plasma hydrolysis or of NADPH-dependent hepatic metabolism.
We synthesized and screened the major 4b and C1 metabolites present in plasma (M1, M2 and M3) for HDAC activity in the biochemical and cellular HDAC assays in order to determine if these products could contribute significantly to HDAC inhibitory activity in vivo. As C1 itself retained no HDAC inhibitory activity, it was unsurprising that M3 was similarly inactive. The M2 metabolite of 4b was also inactive, which was again unsurprising given the hydrolytic cleavage of the benzamide warhead. On the other hand, M1 did retain some very weak activity against HDAC3 (IC50 = 26 µM under standard 1 h pre-incubation conditions; data not shown), but was totally inactive in the Class I cellular HDAC assay up to 50 µM tested (5 h incubation). It is therefore highly unlikely that the major circulating metabolites of 4b would sufficiently impact HDAC inhibition in vivo.
4b was stable in incubations in simulated gastric fluid, with a half-life >10 h, and only 5% turnover observed in 4 h, suggesting that stability within the gut would not be an issue. The permeability and the potential of compound 4b to be a substrate of the efflux transporter P-glycoprotein (Pgp) were evaluated in Caco2 monolayers and in MDCK cell cultures. The apparent permeability (Papp) of 4b was moderate-to-good in both cell lines, (Papp A–B = 156 nm/s in Caco2 and 108 nm/s in MDCK) suggesting that the permeability of the compound is unlikely to restrict intestinal absorption. However, 4b was found to be a substrate for Pgp (effective efflux ratios of 4.9 and 3.5 in MDCK overexpressing MDR1 and Caco2, respectively).
Degradation of 4b to C1 was observed under acidic conditions, for example with the HCl or TFA salt, and was not a product of enzymatic metabolism. M3, the amide hydrolysis product, was the only product observed in incubations of C1 in plasma (Fig. 3B), and the major product formed in incubations of C1 in liver microsomes (data not shown). C1 was extremely unstable in mouse liver microsomes incubated with or without NADPH, with half-life of <5 to 7 min respectively, resulting in a very high predicted in vivo plasma clearance of >2.89 L/h/kg, equivalent to liver plasma flow (data not shown).
Taken together, these data strongly suggests that 4b will be stable in the stomach and will reach the intestinal wall when dosed in vivo. The oral bioavailability of 4b will be lower than via parenteral routes due to Pgp-mediated efflux, and the compound will undergo significant metabolism in the intestine and liver during the absorption process (first pass metabolism). 4b reaching the systemic circulation will be rapidly and extensively metabolised during absorption by amidases and CYP enzymes, and in systemic circulation by plasma amidases, resulting in high clearance.
Following subcutaneous (sc) administration, early concentrations of 4b are expected to be higher than those observed with an oral dose as the impact of first pass metabolism will be reduced. However, extensive metabolism of 4b is to be expected once 4b has reached the systemic circulation. If indeed 4b distributes to brain, its concentrations will likely be very low to negligible since it will be actively transported out of the CNS via the Pgp transporter system.
Formulation and Acute Pharmacokinetic (PK) Profile of 4b
The chemical instability of the salt forms in aqueous solutions, and the insolubility of 4b prepared as a free base, resulted in unacceptable formulations to interrogate any in vivo efficacy of 4b with the previously used procedures. As a consequence de novo formulation attempts were made with the stable free base form. After several trials with different excipients, we were able to reach a soluble formulation preparation of 1 mg/ml 4b in 2% N-methyl-2-pyrollidone, 18% polyethylene glycol 400, 10% solutol HS-15 and 70% (1% Lutrol F68 in water), a vehicle which we have previously used without any adverse effects in R6/2 model chronic efficacy trials, but is unlikely to be palatable to mice in drinking water. With this concentration, a single oral gavage dose will result in a maximum of 10 mg/kg dose level (assuming a maximum dose volume of 10 mg/mL), considerably lower than that estimated to have been used by Thomas et al (150 mg/kg day in drinking water) . In an attempt to replicate the original dose level as close as possible, it became necessary to formulate the test compound in suspension. We achieved a stable homogeneous suspension of free base 4b up to 100 mg/ml in 0.5% carboxymethylcellulose (medium viscosity) in reverse osmosis deionized (RODI) water. This suspension was used to evaluate the oral pharmacokinetics of 4b in mice.
Given the in vitro metabolism profile of 4b, of particular interest to us was an evaluation of oral exposure, so that inferences could be drawn between plasma concentrations achieved with parenteral versus oral administration between the acute pharmacodynamic trials previously used (150 mg/kg s.c qd in 4b. TFA in 50:50 DMSO/PBS), and the oral drinking water formulation (1 mg/mL 4b. TFA complexed with 2-hydroxypropyl-β-cyclodextrin – estimated daily dose of 150 mg/kg) . In addition, we determined the brain concentration of 4b over time in order to estimate whether pharmacologically relevant levels were reached.
The pharmacokinetic parameters of 4b following dosing with either 5 mg/kg subcutaneous (sc) injection using the soluble formulation, or 4b per oral (po) by gavage (50 mg/kg in suspension) in mice plasma and whole brain homogenate are shown in Fig. 4 and Table 1. Triplicate dose formulation samples were retained and analyzed to ensure an accurate concentration of 4b was injected. The sc dose was calculated to be on average 84.3% of the 5 mg/kg target dose. Therefore, for PK analysis purposes, the dose level for this group was adjusted to 4.22 mg eq./kg based on the reported average dose formulation concentration and calculation parameters were adjusted accordingly. The po gavage dose was accurate at 50 mg/kg (within 104% of the nominal concentration of 10.0 mg/mL), and triplicate sampling from the vial (1 per strata; top, middle, and bottom) confirmed that the suspension dosed was homogeneous.
(A) Plasma concentrations of 4b were monitored between 5 min and 24 h after a single subcutaneous (4.22 mg/kg eq) or single oral dose (50 mg/kg) of 4b. Amount of drug in plasma was below the limit of quantitation (LLQ = 3.07 nM eq.) after 4 h (sc) and 8 h po. (B) Equivalent brain concentrations of 4b were monitored at 0.5, 1, 8 and 24 h in the same study. Amount of 4b in brain was much lower than plasma and below the limit of quantitation (LLQ = 2.3 nM eq.) after 8 h by both routes.
The plots of mean 4b plasma and brain concentrations over time for each group are shown in Fig. 4. Following both sc (4.22 mg/kg) and po (50 mg eq/kg) administration, 4b was rapidly absorbed with Tmax occurring at ≤0.5 h. However, in line with the in vitro metabolism data, the plasma exposure was very significantly less after oral administration, bearing in mind the 11.8 times higher po vs sc dose level, resulting in a decrease in Cmax from 1.34 µM to 1.3 µM and AUC decrease from 1.63 µM to 1.08 µM•h. Thus the bioavailability of the compound is ~12 to 18-fold less through oral suspension dosing versus subcutaneous administration. Concentrations of 4b in brain tissue were measurable for all animals through 1 h following subcutaneous dosing and through 8 h following oral dosing. As expected due to 4b’s Pgp substrate liabilities, brain-to-plasma ratios were very low (Table 2), ranging between 0.053 and 0.18 for both dose groups (determined up to 1 h following sc dosing and 8 hr following po dosing), with an observed brain Cmax of 24 nM, by oral administration, and 134 nM by sc administration. Concentrations of 4b in the brain were below the lowest limit of quantitation (LLOQ = 0.76 nM ) of the assay after 1 h (sc) or 8 h (po).
4b partitions into erythrocytes (blood-to-plasma ratio ~3; data not shown), thus concentrations of 4b in mouse whole blood (and corresponding exposures), are approximately 4-times higher than those reported here for plasma. Brain concentrations, however, are not affected; in fact, brain-to-blood concentration ratios become even lower, and the low concentrations of 4b observed in brain can be attributed to residual blood in the brain of these non-perfused animals.
Central Pharmacodynamic (PD) Evaluation of 4b
Despite the low probability of achieving a functional inhibition of Class I HDAC activity in CNS following oral administration of 4b, we set out to directly interrogate this by monitoring histone acetylation patterns. Compound 4b was administered as a free base in suspension via repeated twice daily oral gavage of 150 mg/kg for 5 days to achieve a maximal dose. Although unlikely, any potential accumulation of 4b in brain tissue during the repeated administration procedure could possibly explain the efficacy previously observed in R6/2 and attributed to central Class I (HDAC3) inhibition. Dose formulation analysis confirmed that all doses formulated were on target (104±1.5% of nominal concentration), and replicates from within different strata of each vial analyzed (top, middle and bottom) confirmed dose homogeneity (data not shown). At the end of the study, mice were sacrificed at putative brain Tmax (1 h post last dose) based on the previous PK observations. Cortical tissue from the mice was assessed using specific antibodies for increases in acetylation of specific lysine residues on Histones H3 (H3K4, H3K9, H3K14) and H4 (H4K5) as well as global acetylation of H3 (Ac-H3) and H4 (Ac-H4). The acetylation level was normalized to total H3 and H4 expression level. The samples from the rest of the brain were analyzed for terminal 4b levels at the time of sacrifice. As a positive control, a separate cohort of mice was injected sc with 200 mg/kg SAHA according to previous procedures  and sacrificed at the same time for evaluation of histone acetylation. As illustrated in Fig. 5, dosing of 4b at 150 mg/kg po bid for five consecutive days failed to enhance histone acetylation. In contrast, a robust enhancement of acetylation of H3K9, H4K5, and total acetylated levels of H3 and H4 were observed with SAHA, in line with previous observations . In agreement with the lack of an observable central pharmacodynamic response to the administration of 4b, concentrations of 4b in the “rest of the brains” collected (n = 12 of 14) were below the limit of quantitation (LLQ was 5 nM), in this study in all but two animals examined. These animals demonstrated low but detectable brain concentrations at 20 and 23 nM, respectively, which may be attributed to residual blood in the brain of these non-perfused animals. These exposure data are in good agreement with the previous PK analysis showing negligible brain penetration of the compound with 50 mg/kg po.
(A) Representative immunoblot showing histone acetylation in mouse brain in response to 4b treatment. Mice treated with SAHA were used as a positive control (B). Acetylation at specific lysine residues on histone 3 (H3K4, H3K9, H3K14) and Histone 4 (H4K5) as well as global acetylation of H3 (Ac-H3) and H4 (Ac-H4) were studied using specific antibodies. Acetylation level was normalized to H3 and H4 expression level. (C) and (D) Quantification of (A) and (B) respectively. **P<0.01, *P<0.05 versus vehicle (veh). n = four per treatment. Error bars indicate SEM.
Due to the published in vitro and in vivo results with the pimelic diphenylamide HDAC inhibitors in cell and mouse models of FRDA and HD, we evaluated the HDAC isoform selectivity, cellular activity, in vitro and in vivo ADME properties of the preclinical prototype compound HDACi 4b to validate and extend previous findings and assess its therapeutic potential for HD. Our data on the in vitro selectivity and binding mode of this compound largely agree with previous reports for the closely-related analogue 106, which demonstrates a unique slow-on/slow-off binding mode of these HDAC Class I inhibitors relative to hydroxamic acid-based HDAC inhibitors. The association of 106 to HDAC3 association was previously reported to proceed considerably more slowly than the association to HDAC1. Dissociation rates of the complex also differed; for the 106:HDAC3 complex, the half-life (at room temperature) was ~6 h, whereas it was ~1.5 h for the 106:HDAC1 complex . In that study, a progression method was used to assess the Ki values of 106 binding to HDACs, resulting in a kinetic profile for 106 of HDAC3 (Ki 14 nM) > HDAC2 (Ki 102 nM) > HDAC1 (Ki 148 nM), quite different from the selectivity profile of HDAC1 (IC50 = 150 nM) > HDAC3 (IC50 = 370 nM) > HDAC2 (IC50 = 760 nM), measured using conventional assays with compound-enzyme incubation times of 1 to 3 h .
Bressi et al have since proposed a model (following crystal structure determination of a ortho-N-acyl-phenylene diamine inhibitor bound to HDAC2) in which disruption of an intramolecular hydrogen bond of the NH2 group to the carbonyl oxygen is required for this tight binding and could be responsible for the slow-on/slow-off kinetics . A publication by Xu et al further defined 106 activity as being highly preferential for HDAC3 inhibition over HDAC1 and HDAC2. This group synthesized the chemical probe 1-BP, consisting of a benzophenone photolabeling group attached through a flexible ethylene glycol linker to 106 plus an alkyne group for subsequent attachment of an azide-linked reporter dye for affinity capture . 1-BP retained HDAC inhibitory activity against recombinant HDACs 1, 2, and 3 equivalent to 106. 1-BP was subsequently used for HDAC isoform target identification when incubated with recombinant HDACs followed by irradiation to effect photo cross-linking, fluorescent dye attachment by click chemistry and gel electrophoresis. The 1-BP: HDAC3 interaction was by far the strongest association seen, with much lower association of 1-BP: HDAC1 being the only other HDAC interaction noted, and only when higher enzyme concentrations were used.
This result  appeared at odds with the earlier publication  reporting good HDAC1 and HDAC2 inhibition following prolonged incubation of enzyme with benzamide 106. The authors speculated that the increased stability of the 106:HDAC3 complex accounted for the difference in cross-linking activity of 1-BP for these enzymes, and concluded that HDAC3 was the preferred cellular target of the pimelic diphenylamide inhibitor 106 used in the in vivo FRDA mouse models, which is very closely related in structure to 4b used in the R6/2 HD mouse model . They also speculated that the efficacy of the pimelic diphenylamide inhibitors versus the lack of efficacy of the hydroxymate inhibitors to increase FXN expression in published reports was due to the absolute requirement of this stable HDAC3: inhibitor complex.
In our study, our ‘functional’ deacetylase inhibition data shows a modest selectivity for HDAC3 over HDAC1 and HDAC2, which in our opinion is not a sufficient pharmacological basis to identify HDAC3 as the exclusive target for 4b actions in vivo. In addition to reconfirming the biochemical profile of 4b as exemplifying the novel mode of action (slow-on/slow-off) of pimelic diphenylamide HDAC inhibitors for Class I HDACs, our data provides insight into the cellular inhibitory profile of endogenous Class I HDAC inhibition and helps define the concentrations required in plasma or brain to effectively inhibit the target in a native cell environment. In our study, a maximal cellular IC50 for ‘Class I’ HDAC inhibition of 1.8 µM after 24 h incubation was achieved and used to benchmark in vivo central target engagement. Thus, an in vivo proof of concept study linking functional central inhibition of Class I HDACs to efficacy in a disease state should ideally provide confirmation of at least some correlation between significant target engagement and phenotypic outcome, to support pursuit of this approach in a clinically afflicted population.
We further characterized the in vitro ADME profile of 4b to explore any metabolic liabilities that would inform subsequent in vivo efficacy testing and dosing schedule. Our findings indicate that 4b is very unstable in plasma (T1/2 1.9 hr) and in liver microsomes (T1/2 with NADPH as cofactor ~20 min) resulting in a very high predicted in vivo plasma clearance of 2.6 L/h/kg (approximately 87% liver plasma flow). The main hydrolysis product in plasma is M1, which accounts for 78% of the metabolism of parent, followed by M2, which accounts for 10% of the entire metabolism. Our biochemical and cellular profiling of these two metabolites confirmed that neither inhibits HDACs in a cellular setting (up to 50 µM), confirming that these metabolic products of 4b would not inhibit HDACs in vivo. In summary, based on our in vitro ADME data, we predict that 4b will be rapidly metabolised by plasma and hepatic amidases and other hepatic enzymes in vivo, significantly limiting systemic and CNS availability. Additionally, we show that 4b is a substrate for Pgp in MDCK cells overexpressing MDR1 (efflux ratio (ER) of 4.9), in agreement with the efflux determined in Caco2 monolayers (ER 3.5). This in vitro data predicts a limited oral bioavailability and very minimal CNS exposure for compound 4b.
Despite an unfavourable predicted in vivo pharmacokinetic profile, we pursued further in vivo studies in light of the published positive efficacy study . Surprisingly, 4b did not dissolve in the previously described formulation . Unlike SAHA, which dissolves in cyclodextrins upon heating and is stable for seven days at room temperature , investigation of both free-base and salt forms of 4b showed that neither was capable of full dissolution to 1 mg/mL as previously described. However, the physicochemical instability of the acidic salts of 4b, combined with the efficient and irreversible conversion of the parent compound to the inactive benzimidazole C1, might explain the disparity between the published studies and our data. The benzimidazole C1 is readily soluble in aqueous media and the conversion process is exacerbated under acidic conditions and at elevated temperature. Hence, it is likely that there was very significant contamination of C1 in the chronic drinking water preparations previously used.
In agreement with our in vitro ADME predictions, 4b reaching the systemic circulation was rapidly cleared, which resulted in a plasma half-life of approximately 40 min after subcutaneous dosing. Subcutaneous dosing resulted in much higher relative concentrations in plasma and brain tissue as the impact of first pass metabolism and efflux by MDR1 was reduced via this route.
To put these results in the context of the previous study , the subcutaneous administration is easier to interpret, since both studies used 4b in soluble form for acute administration, which largely sets aside any salt versus free-base stability disparities. Indeed, a comparative stability analysis of 4b and 4b•TFA prepared at 1 mg/ml in DMSO showed both are soluble, but the TFA salt again showed signs of degradation (day 7 values being 59% of the value measured at day 1 after formulation). According to Thomas et al, verification of an acute pharmacodynamic response (the reversal of transcriptional dysregulation in the R6/2 model) was achieved by the repeated once daily injection of 150 mg/kg 4b in 50:50 DMSO: PBS for three consecutive days. Assuming linear kinetics and based on our values when 4b was dosed at 4.22 mg/kg (s.c), the brain Cmax achieved in that study would be in the region of 4–5 µM ( = (150 mg/kg/4.22 mg/kg)* Cmax of 134 nM)), sufficient to potentially fully inhibit Class I HDAC cellular activity, based on our cellular in vitro potency values (Fig. 1), and consistent with the positive results obtained. However, given the ≥12 fold lower exposures shown here from the oral administration versus the sc route, we would expect to achieve a brain Cmax of only ~72 nM ( = (150 mg/kg/50 mg/kg)* Cmax of 24 nM)) via equivalent oral bolus administration of 150 mg/kg. This estimated Cmax is approximately 25 fold lower than the most potent cellular HDAC in vitro IC50 value we measured (1.8 µM). With the slower continuous administration of 4b via a drinking water study (even with the best case assumption that the 4b was stable and fully dissolved in the drinking water), we would expect the Cmax achieved to be significantly lower. The lack of any pharmacodynamic response predictive of central Class I HDAC inhibition when 4b was dosed orally at 150 mg/kg twice daily for 5 days confirmed this prediction.
In conclusion, our findings demonstrate that the physicochemical properties, metabolic and P-glycoprotein substrate liabilities of 4b render it unsuitable as a molecular tool to investigate central Class I HDAC inhibition in vivo in mouse by oral administration. In the pivotal proof of concept trial , 4b was given to R6/2 mice in drinking water, leading to improved behavioral phenotypes. We conclude that this is highly unlikely to be due to HDAC inhibition in the CNS or related to the finding of the reversal of transcriptional dysregulation detected in the acute pharmacodynamic trial , where brain concentration of 4b was most likely ~65 fold higher. Our results cast serious doubts on the validation of CNS HDAC3 as a target for the treatment of HD. Our findings are consistent with Moumné et al , who demonstrated that a genetic cross of Hdac(+/−) heterozygotes with R6/2 mice effectively reduced nuclear HDAC3 levels, but did not ameliorate physiological or behavioural phenotypes and had no effect on molecular changes including dysregulated transcripts. We cannot rule out that a metabolite of 4b or C1 was responsible for the therapeutic benefit seen in R6/2 mice per oral dosing of 4b in the previous study. To our knowledge, no data has been published on the ADME properties of 4b or related compounds used in the FRDA mouse models. Our results underscore the absolute necessity for appropriate ADME evaluation of compounds prior to in vivo target validation.
Materials and Methods
All animal work was conducted according to relevant national and international guidelines. The pharmacokinetic analysis of HDACi 4b was conducted at Charles River Laboratories (CRL), which is subject to legislation under the Animal Welfare Act. At CRL all animal studies are governed by CRL’s Institutional Animal Care and Use Committee (IACUC). CRL is fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC), and is registered with the United States Department of Agriculture (USDA). All procedures involving animals were conducted humanely and were performed by or under the direction of trained and experienced personnel. The protocol was reviewed and approved by the IACUC of CRL prior to study initiation. The veterinarian was consulted in the overall study design for this study type. The pharmacodynamic evaluation of HDACi 4b was approved by the King's College London Ethical Review Panel and experimental procedures were performed in accordance with the UK Home Office regulations.
Synthesis of HDACi 4b
Commercially available ethyl hydrogen pimelate was coupled using EDC to aniline. The resultant amido ester was saponified to afford the amido acid, which was then coupled with phenylenediamine using EDC. The desired product was isolated from the reaction mixture by the slow addition of water and filtration of the solid to give the crude final compound 4b. Analysis of this material by LCMS showed it to contain a minor amount (6% UV area) of the bis-capped material, which was removed by dissolving the crude material in aqueous methanolic HCl, filtration of the insoluble bis-amide and basification with aqueous sodium bicarbonate to give 4b free base in excellent LC purity, with no UV active impurities. A final recrystallisation from ethanol provided pure 4b.
Preparation of TFA and HCl Salt Forms of 4b
The trifluoroacetate (TFA) salt of 4b was prepared from a solution of the free base in methanol which was treated with 0.9 equivalent TFA, and evaporated to dryness without heating. LCMS analysis of this solution showed that in forming the salt, we had introduced a minor contaminant into the mixture, which we postulated was likely to be the cyclised benzimidazole C1.
In order to evaluate the solubility and stability of alternative salt forms, we also prepared 4b as the hydrochloride (HCl) salt. This was made by dissolving 4b free base in a mixture of ethanol-water-c.HCl, followed by evaporation of all volatiles, and azeodrying with heptanes to give a free-flowing white solid. Again, this batch of 4b contained a small amount of C1. Indeed, obtaining a clean sample of 4b. HCl proved to be difficult as it readily cyclised when heated, making recrystallisation impossible. Purification was achieved by multiple slurries of the sample in ethanol.
Preparation of Benzimidazole C1
An authentic sample of the benzimidazole C1 was prepared by heating 4b in ethanolic HCl until cyclization was complete by LCMS. Evaporation of this solution gave compound C1 as the HCl salt in high purity.
Characterization of Biochemical and Cellular Potency Against HDACs
To assess the biochemical and cellular potency of 4b and its conversion products/metabolites, we utilized the two-step fluorogenic assay which measures the HDAC-mediated catalytic conversion of synthetic acetylated lysine substrates. In brief, on deacetylation of the substrates by HDAC activity, a protease site is unmasked which allows subsequent trypsin-mediated cleavage and release of the highly flourescent AMC molecules in a subsequent step of the assay . The substrates used were Lys_Ac_AMC (Bachem I-1875) for HDAC3 and (Ac)Arg-Gly-Lys(Ac) (Bachem I-1925) for HDAC1 and HDAC2. The final substrates used for each enzyme assessment were carefully selected after assessing the catalytic turnover of each of these substrates against HDAC1, HDAC2 and HDAC3 respectively, and selecting the ones that performed best. To assess Class IIa enzyme activity (HDAC4, 5, 7, 9) and Class I HDAC8 activity, we used the alternative substrate Boc_Lys_TFA (Bachem I-1985), equivalent to substrate 4 of . Class IIa HDACs have approximately 1000-fold less catalytic activity than Class 1 enzymes, and show only extremely low turnover of acetylated substrates due to a Tyr to His mutation in the active site . However, the relatively labile and sterically more demanding trifluoracetyl group is readily hydrolyzed by the catalytically less avid Class IIa enzymes, allowing measurement of Class IIa activity using the 2-step fluorogenic assay. Intriguingly the Boc_Lys_TFA substrate seems to show almost total selectivity for Class IIa enzymes over class I and Class IIb enzymes, with the exception of HDAC8 (, and internal data).
For evaluation of the class selectivity of the compounds, we assessed inhibition against purified recombinant human full length HDAC1 (cat# 50051), HDAC2 (cat# 50052), HDAC3-NcoR2 (cat# 50003) and HDAC8 (cat# 50008): as representative of Class I activity; catalytic domain HDAC4(αα 648–1057), HDAC5 (αα 657–1123; cat # 50005), HDAC7 (αα 518-end; cat # 50007) and HDAC9 (αα 604–1066; cat # 50009) activity: representing all Class IIa activity; and full length HDAC6 (cat#50006): representing Class IIb activity. With the exception of purified catalytic domain HDAC4, which was prepared for us by Emerald Biosciences (Seattle, WA), all HDAC enzymes were purchased from BPS Bioscience (San Diego, CA). All purified enzyme preparations were checked for cross-reactivity with antibodies specific to other HDACs, and were found to be pure of other HDAC contamination (data not shown).
Unless otherwise stated in the text, assays were run in the following format in 384-well plates using automated liquid handling procedures. Briefly, frozen enzyme stock and compounds were diluted in assay buffer (50 mM Tris-HCl, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2 at pH 8.0) and added to plates at concentrations sufficient to achieve a 16-point concentration-response curve from 50 µM to 1 nM at 1% DMSO final concentration, after addition of all reagents. Substrate diluted in assay buffer was then added and plates were incubated after brief shaking for 60 min at 37°C. Following incubation, a developer/stop-step was introduced to terminate the reaction and cleave the fluorescent substrate: either the addition of trypsin with 10 µM Compound 26  for the Class IIa enzymes, or trypsin with 5 µM Trichostatin A (TSA) for the Class I/IIb enzymes. Plates were briefly shaken and then returned to the incubator for a further 60 min, followed by measurement of fluorescence per well (Ex 355 nm, Em 460 nm) on a Perkin Elmer EnVision. Enzyme and substrate concentrations were carefully chosen to run all reactions at substrate concentrations of 1 to 2×Km. Final enzyme (E) and substrate (S) concentrations for the reactions were as follows,; HDAC1 (0.8 µg/ml E, 25 µM S), HDAC2 (0.8 µg/ml E, 25 µM S), HDAC3-NcoR2 (0.6 µg/ml E, 25 µM S), HDAC4 (0.6 µg/ml E, 25 µM S); HDAC5 (0.4 µg/ml E, 10 µM S), HDAC6 (0.8 µg/ml E, 6 µM S), HDAC7 (0.05 µg/ml E, 10 µM S); HDAC8 (0.8 µg/ml E, 8 µM S). Signal to background S:B (maximum response 1% DMSO/total inhibition with reference compound) ranged from 7 (HDAC3) to 27 (HDAC8), and Z’ parameter for all assays were between 0.7–0.82 throughout the study.
Due to the cellular permeability of both Boc_Lys_Ac and Boc_Lys_TFA, we additionally used these substrates to respectively address Class I/IIb activity versus Class IIa/HDAC8 activity in a cellular context, taking advantage of their previously demonstrated substrate selectivity . Briefly, Jurkat E6.1 cells (obtained from the European Collection of Cell Cultures (ECACC)) were plated into 384 well plates at 75,000 cells/well in cellular assay buffer (RPMI without phenol red, 0.1% Fetal Bovine Serum, 10 mM HEPES, 1 mM Sodium Pyruvate). Compounds of interest were diluted in assay buffer (16-point concentration curve, 1% final DMSO) and were added to wells - unless stated otherwise in the results section - for a 2 h preincubation at 37°C prior to the addition of either 100 µM Boc_Lys_TFA or Boc_Lys_Ac. Cells were incubated for a further 3 h at 37°C, followed by the addition of a trypsin (1 mg/ml) and reference compound (Compound 26 or TSA) stop-step overnight to terminate the reaction and lyse the cells. Fluorescent counts were read the following morning on the Perkin Elmer EnVision. Z’ was 0.8 for both assays, with S:B of 19 for Boc_Lys_Ac assay, and 7 for Boc_Lys_TFA assay.
Each compound was tested in duplicate in concentration-response format on 3 separate days. For both biochemical and cellular assays, data is presented as % inhibition from maximal activity (maximal fluorescence response), which was defined as the fluorescence read in the presence of 1% DMSO, with 100% inhibition defined as the fluorescence read in the presence of substantial excess of the respective reference compounds (5 µM TSA, 50 µM Compound 26 depending on target). A full dose response of reference compound was always included alongside the profiling, to ensure that assay parameters remained within acceptable limits.
Plasma stability (half-life).
Incubations of test compound (5 µM initial concentration, n = 2) were carried out with pooled mouse plasma or BSA (45 mg/mL in 0.1 M phosphate buffered saline). The incubations were performed at 37°C. Samples (50 µL) were obtained from the incubation at 0, 10, 30, 120, 240, 360 and 1440 min, and added to 150 µL of acetonitrile containing carbamazepine as analytical internal standard to terminate the reaction. Samples were centrifuged and the supernatant fractions analysed by LC-MS/MS.
For half-life determinations, the instrument responses (peak areas) normalized for internal standard response, were referenced to the zero time-point samples (as 100%) in order to determine the percentage of compound remaining. Ln plots of the % remaining, for each compound, were used to determine the half-life for the plasma incubations. Half-life values were calculated from the relationship:-.Where λ was the slope of the natural logarithmic (Ln) concentration vs time curve.
When quantification was required, calibration standards for parent compound and metabolites were prepared in control plasma and extracted and analysed as described for the study samples. Quantification of parent compound or metabolite was by extrapolation from the calibration line.
Hepatic microsomal stability (half-life).
Incubations of test compounds (1 µM initial concentration, n = 2) were carried out with pooled hepatic microsomes (0.25 mg protein/mL in 0.1 M phosphate buffer pH 7.4). NADPH (1 mM) was added to initiate the reactions. The incubations were performed at 37°C. Samples (100 µL) were taken from the incubation at 0, 5, 10, 20 and 40 min and added to 100 µL of acetonitrile containing carbamazepine as analytical internal standard, to terminate the reaction. Samples were centrifuged and the supernatant fractions analysed by LC-MS/MS. The instrument responses (i.e. chromatographic peak heights), normalized by internal standard response were referenced to the zero time-point samples (as 100%) in order to determine the percentage of compound remaining.
Ln plots of the % remaining, for each compound, were used to determine the half-life for the microsomal incubations.
When quantification was required, calibration standards for parent compound and metabolites were prepared in control hepatic microsomes and extracted and analysed as described for the study samples. Quantification of parent compound or metabolite was by extrapolation from the calibration line.
Simulated Gastric Fluid Stability
Simulated gastric fluid (SGF), pH 1.2, was prepared containing 2 g/L sodium chloride, 3.2 g/L of pepsin, 0.7% (v/v) HCl. Gastric buffer, pH 1.2, was also prepared containing 2 g/L sodium chloride and 0.7% (v/v) HCl. Test compound (10 µM) was added to both fluids and samples were mixed at room temperature on an orbital shaker. At each time-point (0, 2 and 4 h) 50 µL of sample was added to 150 µL DMSO, mixed and analysed immediately. Samples were analysed by LC-UV (λ = 254 nm. The instrument responses (i.e. chromatographic peak areas) were referenced to the zero time-point samples (as 100%) in order to determine the percentage of compound remaining at each time-point.
Permeability and Effective Efflux Ratio in Caco2 and MDCK-MDR1
Caco-2 cells (obtained from ECACC) were used after a 21-day cell culture period in 24-well Transwell plates (seeding density of 2×105 cells/well). Test compounds (10 µM) were dissolved in Hanks’ Balanced Salt Solution (HBSS) containing 25 mM HEPES (pH 7.4) and added to either the apical or basolateral chambers of a Transwell plate assembly. Duplicate wells were prepared per assay condition. Lucifer Yellow was added to the apical buffer in all wells to assess integrity of the cell layers by monitoring Lucifer Yellow permeation. As Lucifer Yellow (LY) cannot freely permeate lipophilic barriers, a high degree of LY transport indicates poor integrity of the cell layer.
After 1 hr incubation at 37°C, aliquots were taken from the acceptor chamber of each Transwell and added to acetonitrile containing analytical internal standard (carbamazepine) in a 96 well plate. Concentrations of test compound in the samples were measured by high performance liquid-chromatography/mass spectroscopy (LC-MS/MS).
Donor = Chamber of Transwell to which compound is dosed: apical for A>B experiments and basal for B>A experiments.
Acceptor = Chamber of Transwell in to which permeation of compound is measured: basal for A>B experiments and apical for B>A experiments.
The Efflux ratios, as an indication of active efflux from the apical cell surface, were calculated using the ratio of Papp B>A/Papp A>B.
The MDR1-MDCKII and wild type MDCKII cell lines (obtained from SOLVO Biotechnology) were cultured in accordance with the guidelines provided by SOLVO Biotechnology. Both wild-type MDCK and MDR1-MDCK cells were seeded at a cell density of 2.3×105 cells/well into 24-well Transwell plates and cultured for three days to form monolayers. Test compound was loaded into the donor compartments of the Transwell plate (24-well) bearing MDR1-MDCK or wild type MDCK monolayers. Test compound was added to either the apical or basolateral chambers of the Transwell plate assembly at a concentration of 10 µM in Hanks’ Balanced Salt Solution containing 25 mM HEPES (pH 7.4). Lucifer Yellow was added to the apical buffer in all wells and its permeation monitored to assess integrity of the cell layer. As Lucifer Yellow (LY) cannot freely permeate lipophilic barriers, a high degree of LY transport indicates poor integrity of the cell layer and wells with LY permeability above 100 nm/s are rejected.
After 1 h incubation at 37°C, aliquots were taken from both chambers and added to acetonitrile containing analytical internal standard (carbamazepine) in a 96-well plate. Concentrations of compound in the samples were measured by LC-MS/MS. Concentrations of LY in the samples were measured using a fluorescence plate reader.
The apparent permeability (Papp) values of test compound were determined for both the apical to basal (A>B) and basal to apical (B>A) permeation and the efflux ratio (B>A: A>B) determined in both the wild type MDCK and MDR1-MDCK cells.
Donor = Chamber of Transwell to which compound is dosed: apical for A>B experiments and basal for B>A experiments.
Acceptor = Chamber of Transwell in to which permeation of compound is measured: basal for A>B experiments and apical for B>A experiments.
The Efflux ratios, as an indication of active efflux from the apical cell surface, were calculated using the ratio of Papp B>A/Papp A>B.
The effective efflux ratio was also determined from the ratio observed in MDR1-MDCK cells relative to the ratio observed in wild-type cells. Known substrates for human MDR1 typically display effective efflux ratios of greater than two.
Incubations (n = 2) of test compounds (1 µM initial concentration; 0.5 mg protein/mL) or DMSO control were performed in mouse liver microsomes with and without NADPH, as described above. Aliquots were taken for at 0 and 60 min, added to an equal volume of acetonitrile and submitted for analysis. All incubations (including DMSO control incubations) were analysed by LC-MS.
Analytes were separated by UPLC and analyzed by mass spectrometry. Chromatographic separation was achieved with a Waters Acquity UPLC BEH C18 column (2.1×50 mm×1.7 µm), the injection volume was 2 µL and the flow rate was 0.6 mL/min. Mobile Phase B was held isocratic at 5% B for 0.2 min, and increased linearly to 95% in 1.2 min. The column was washed with 95% B for 0.6 min, and equilibrated to starting conditions (5% B), for 0.2 min. Mobile Phases were A (0.01% formic acid in water) and B (0.01% formic acid in methanol). A Waters Xevo-TQ mass spectrometer (Waters Ltd, Centennial Park, Hertfordshire) was used for metabolite identification, with an electrospray source at following settings: capillary voltage = 3.5 kV, cone voltage = 35 V, extractor voltage = 1.6 V, source temperature = 150°C, desolvation gas temperature = 500°C, desolvation gas flow = 1000 L/h, cone gas flow = 100 L/h and collision gas flow = 0.15 mL/min.
Incubation extracts were scanned over a mass range of 50 to 1000 amu in both positive and negative ionisation modes. Mass chromatograms were generated for ions observed in the extracts from incubated compounds relative to DMSO controls.
In addition, single ion recording (SIR) methods were set up for metabolites thought likely on the basis of the structure of the test compounds. These included likely hydrolysis and oxidation products and also for oxidative metabolic products considered likely based on the compound structures. Where chromatographic peaks of greater intensity than controls were confirmed in the incubated samples, daughter (fragmentation) spectra were obtained and structures proposed for putative metabolites based on the fragmentation patterns when compared to those of parent compounds.
Daughter (product) ion scans of putative metabolites were performed using four collision energies (10 eV, 20 eV, 40 eV and 60 eV) to generate fragment spectra. These were compared in order to assess the likely regions at which metabolism had occurred.
Pharmacokinetic Evaluation of 4b
The pharmacokinetic study was conducted at Charles River USA.
Dose formulation preparation.
All dose formulation procedures were conducted under yellow lighting conditions to protect 4b from light. For the subcutaneous dose group, 2.504 mg 4b was weighed, and transferred into a formulation container. NMP (0.05 mL) was added and the contents were vortexed until 4b was completely dissolved. With continuous vortexing after each addition, PEG400 (0.45 mL), Solutol HS-15 (0.25 mL) and finally 1% Lutrol F68 in RODI water (1.75 mL) followed by sonication for 6 min. The formulation was filtered into a sterile serum vial using a 0.22 µm Millex GV PVDF filter. The resultant formulation was a clear colorless solution.
For the oral dose group, 4b was weighed (21.138 mg) and transferred to a formulation container. The prepared vehicle (0.5% CMC, 2.11 mL) was transferred into the formulation container, and the contents were stirred and sonicated for a total of 20 min. The resultant formulation was a white homogenous suspension.
For sc formulation, quadruplicate retention samples (100 µL each) were collected and placed in polypropylene vials postfiltration. Triplicate samples were stored at 22°C ±5°C, protected from light, For po formulation, triplicate retention samples (100 µL each) were collected (1 per strata; top, middle, and bottom) from the dose formulation after vortexing. The retention samples were placed into polypropylene vials and stored at 22°C ±5°C, protected from light. The retention samples were analyzed 10 days following preparation.
Blood collection and plasma preparation.
Whole blood samples (225 µL each ~90 µL plasma) were collected at each time point per animal into tubes containing K2EDTA. Samples were held on wet ice prior to centrifugation. Mice were bled twice; the first bleed was made via the submandibular facial vein, the second bleed was a terminal collection via cardiac puncture. Whole blood was centrifuged at 2200 × g for 10 min at 5°C ±3°C to separate the plasma. The entire volume of the plasma sample was then transferred to individual tubes contained in a 96-well Matrix plate kept chilled in icy water through the entire process until storage at −70°C ±10°C. Collected plasma samples were stored at −70°C ±10°C until transferred to the analytical laboratory frozen on dry ice for bioanalysis.
Tissue collection and preparation.
The brain of each animal was collected following CO2 euthanasia and terminal bleeding. Each brain was rinsed with saline once removed from the body (to flush away any blood that may have been present), snap frozen in liquid nitrogen, and stored individually in 15 mL conical tubes at −70°C ±10°C.
Preparation of brain homogenate prior to bioanalysis.
Prior to extraction, acetonitrile: water (3:1, v/v) was added to each tissue sample at a tissue: solvent ratio of 1:3 (w/v) or 1:2.536 (w/w), and homogenized using an OMNI tissue homogenizer. The actual volume of extraction buffer or organic solvent added to each tissue was recorded. Samples were kept chilled in icy water through the entire process until processed for 4b extraction. Immediately prior to extraction, tissue homogenates were thoroughly vortexed and aliquoted into 96-well plate(s). The entire process, from tissue homogenization to placing the samples in the tubes for 4b extraction, was conducted within 8 h.
Preparation of external calibration standards to construct a standard curve 4b.
primary stock solution was prepared at 1 mg eq./mL in DMSO. The matrix calibration standards were prepared at 1, 2, 5, 10, 50, 250, 1000, 2500, 5000 and 10000 ng eq./mL in mouse plasma for plasma, and 0.25, 0.5, 1.25, 2.5, 12.5, 62.5, 250, 625, 1250, 2500 ng eq./mL in tissue homogenate for tissue analysis. Plasma samples were quantified against an external calibration curve generated in control plasma from male C57BL/6NCRL mice. Tissue samples were quantified against an external calibration standard curve generated in control brain tissue from male C57BL/6NCRL mice.
Extraction of the test article from samples for bioanalysis.
Aliquots (25 µL) of samples, plasma, and tissue blanks (containing internal standard only), plasma and tissue double blanks (without internal standard), control blanks (solvent only), diluted dose (dilute in plasma from the study species prior to extraction) and matrix calibration standards were dispensed into 96-well plates. Extracting solution with internal standard (100 µL) was added to all samples except matrix double blanks and solvent blanks. Extracting solution without internal standard (100 µL) was added to matrix double blanks. Samples were vortexed and centrifuged for 5 min, and the supernatants were transferred to a new plate. An aliquot (50 µL) of Milli-Q water was added to the samples, which were covered and vortexed for 5 min.
A universal method with minimal method development and no validation was performed. Calibration standards were prepared in duplicate for each concentration. At a minimum, 75% of all the calibration standards and at least two calibration standards per concentration met the accuracy and precision of ±30%. There was no bias in the accuracy or precision for the run to be acceptable (ie, an approximately equal number of calibration standards will fall above and below the theoretical values). The coefficient of variation (CV%) of the internal standard signal/area response for the entire run was within ±15%, and there was no bias or trend in the internal standard signal/area response for the run to be acceptable. The front-end and back-end calibration curves were determined. The mean concentrations of the calibration standards at the front end versus those at the back end of calibration curve were within ±15% of each other. Samples above the limit of quantitation were diluted to fall within the calibration range.
Mean concentration values per time point were calculated and were used to calculate the composite pharmacokinetic (PK) parameters. Pharmacokinetic parameters were calculated by non-compartmental analysis using the validated software WinNonlin program, version 5.2 (Scientific Consulting Inc., Palo Alto, California). A model was selected based on the extravascular (subcutaneous and oral gavage) routes of administration. For each route, the concentration at time zero was assumed to be zero. Plasma and tissue concentrations below the limit of quantitation were treated as absent samples for the purpose of calculating the mean plasma concentration values or for calculating pharmacokinetic parameters.
The area under the plasma concentration versus time curve (AUC) was calculated using the linear trapezoidal method (linear interpolation). When appropriate, the terminal elimination phase of the PK profile was estimated using at least the last three observed concentration values. PK parameters describing the systemic exposure of the test article in the test system were estimated from observed (rather than predicted) plasma concentration values, the dosing regimen, the AUC, and the terminal elimination phase rate constant (kel) for each group. The portion of the AUC from the last measurable concentration to infinity was estimated from the equation Ct/kel, where Ct represents the last measurable concentration. The extrapolated portion of the AUC was used for the determination of AUC(0-inf).
Histone Acetylation from Brain Tissue
Mouse brains were harvested, dissected, immediately frozen in liquid nitrogen and stored at −80°C. Mouse brain tissue was homogenized (polytron homogenizer, 20 s) in 2 volumes of histone extraction buffer (1×PBS, 5% Triton X-100 (v/v), 3 mM DTT, 1 mM orthovanadate, 5 mM NaF, 1 mM PMSF, 5 mM Na Butyrate, Roche complete protease inhibitor tablet) and centrifuged at 900 g for 8 min at 4°C. The resulting pellet was washed twice in 1 ml of histone extraction buffer before being re-suspended in 2–3 volumes of 0.2 M HCl. Samples were then vortexed briefly and left to shake vigorously for 3 h at 4°C using a VXR Vibrax 1.5 ml polypropylene tube shaker (IKA). After shaking, samples were centrifuged at 900 g for 8 min at 4°C and the resulting supernatant was retained and neutralized with 0.2 volumes of 1 M NaOH.
Histones were separated by SDS-PAGE using a 15% polyacrylamide gel and transferred to a nitrocellulose membrane using standard western blotting transfer apparatus (BioRad). Histone proteins were then detected using anti-histone H4 (Millipore - 60599 - 1:1000), anti-acetyl histone H4 (Millipore - 60598 - 1:1000), anti-histone H3 (Millipore - 06755 - 1:10,000), anti-acetyl histone H3 (Millipore - 60599 - 1:1000), anti-histone H3 acetyl K4 (Millipore - 07539 - 1:1000), anti-histone H3 acetyl K9 (Abcam - ab10812 -1:1000), anti-histone H3 acetyl K14 (Millipore - 1710051 - 1:1000), or anti-histone H4 acetyl K5 (Millipore - 92590 - 1:1000) antibodies diluted in PBS - 0.1% Tween-20 (v/v).
Displacement of CCK-8S (agonist radioligand) from the CCK1 receptor by 4b and C1.
% inhibition of control specific binding of selected radioligand assays (Cerep diversity profile) by 10 µM 4b or C1.
Conceived and designed the experiments: MB OA KM LM DY GPB CD IMS RWB GM VB. Performed the experiments: OA KM LM COC SC HP JV LC AFH DLS BW FB. Analyzed the data: OA KM MB DY LM COC SC HP JV FB GPB VB. Contributed reagents/materials/analysis tools: FB. Wrote the paper: MB VB.
- 1. Soragni E, Xu C, Cooper A, Plasterer HL, Rusche JR, et al. (2011) Evaluation of histone deacetylase inhibitors as therapeutics for neurodegenerative diseases. Methods Mol Biol 793: 495–508. doi: 10.1007/978-1-61779-328-8_32
- 2. Marmolino D, Acquaviva F (2009) Friedreich's Ataxia: from the (GAA)n repeat mediated silencing to new promising molecules for therapy. Cerebellum 8: 245–259. doi: 10.1007/s12311-008-0084-2
- 3. Grabczyk E, Mancuso M, Sammarco MC (2007) A persistent RNA.DNA hybrid formed by transcription of the Friedreich ataxia triplet repeat in live bacteria, and by T7 RNAP in vitro. Nucleic Acids Res 35: 5351–5359. doi: 10.1093/nar/gkm589
- 4. Wells RD (2008) DNA triplexes and Friedreich ataxia. FASEB J 22: 1625–1634. doi: 10.1096/fj.07-097857
- 5. Herman D, Jenssen K, Burnett R, Soragni E, Perlman SL, et al. (2006) Histone deacetylase inhibitors reverse gene silencing in Friedreich's ataxia. Nat Chem Biol 2: 551–558. doi: 10.1038/nchembio815
- 6. Rai M, Soragni E, Jenssen K, Burnett R, Herman D, et al. (2008) HDAC inhibitors correct frataxin deficiency in a Friedreich ataxia mouse model. PLoS One 3: e1958. doi: 10.1371/journal.pone.0001958
- 7. Miranda CJ, Santos MM, Ohshima K, Smith J, Li L, et al. (2002) Frataxin knockin mouse. FEBS Lett 512: 291–297. doi: 10.1016/s0014-5793(02)02251-2
- 8. Al-Mahdawi S, Pinto RM, Ruddle P, Carroll C, Webster Z, et al. (2004) GAA repeat instability in Friedreich ataxia YAC transgenic mice. Genomics 84: 301–310. doi: 10.1016/j.ygeno.2004.04.003
- 9. Al-Mahdawi S, Pinto RM, Varshney D, Lawrence L, Lowrie MB, et al. (2006) GAA repeat expansion mutation mouse models of Friedreich ataxia exhibit oxidative stress leading to progressive neuronal and cardiac pathology. Genomics 88: 580–590. doi: 10.1016/j.ygeno.2006.06.015
- 10. Sandi C, Pinto RM, Al-Mahdawi S, Ezzatizadeh V, Barnes G, et al. (2011) Prolonged treatment with pimelic o-aminobenzamide HDAC inhibitors ameliorates the disease phenotype of a Friedreich ataxia mouse model. Neurobiol Dis 42: 496–505. doi: 10.1016/j.nbd.2011.02.016
- 11. MacDonald ME, Ambrose CM, Duyao MP, Myers RH, Lin C, et al. (1993) A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes. The Huntington's Disease Collaborative Research Group. Cell 72: 971–983. doi: 10.1016/0092-8674(93)90585-e
- 12. Francis JW, Sandrock AW, Bhide PG, Vonsattel JP, Brown RH Jr (1998) Heterogeneity of subcellular localization and electrophoretic mobility of survival motor neuron (SMN) protein in mammalian neural cells and tissues. Proc Natl Acad Sci U S A 95: 6492–6497. doi: 10.1073/pnas.95.11.6492
- 13. Munoz-Sanjuan I, Bates GP (2011) The importance of integrating basic and clinical research toward the development of new therapies for Huntington disease. J Clin Invest 121: 476–483. doi: 10.1172/jci45364
- 14. Kazantsev AG, Thompson LM (2008) Therapeutic application of histone deacetylase inhibitors for central nervous system disorders. Nat Rev Drug Discov 7: 854–868. doi: 10.1038/nrd2681
- 15. Sadri-Vakili G, Cha JH (2006) Histone deacetylase inhibitors: a novel therapeutic approach to Huntington's disease (complex mechanism of neuronal death). Curr Alzheimer Res 3: 403–408. doi: 10.2174/156720506778249407
- 16. Butler R, Bates GP (2006) Histone deacetylase inhibitors as therapeutics for polyglutamine disorders. Nat Rev Neurosci 7: 784–796. doi: 10.1038/nrn1989
- 17. Steffan JS, Bodai L, Pallos J, Poelman M, McCampbell A, et al. (2001) Histone deacetylase inhibitors arrest polyglutamine-dependent neurodegeneration in Drosophila. Nature 413: 739–743.
- 18. Sadri-Vakili G, Bouzou B, Benn CL, Kim MO, Chawla P, et al. (2007) Histones associated with downregulated genes are hypo-acetylated in Huntington's disease models. Hum Mol Genet 16: 1293–1306. doi: 10.1093/hmg/ddm078
- 19. Bates EA, Victor M, Jones AK, Shi Y, Hart AC (2006) Differential contributions of Caenorhabditis elegans histone deacetylases to huntingtin polyglutamine toxicity. J Neurosci 26: 2830–2838. doi: 10.1523/jneurosci.3344-05.2006
- 20. Taylor JP, Taye AA, Campbell C, Kazemi-Esfarjani P, Fischbeck KH, et al. (2003) Aberrant histone acetylation, altered transcription, and retinal degeneration in a Drosophila model of polyglutamine disease are rescued by CREB-binding protein. Genes Dev 17: 1463–1468. doi: 10.1101/gad.1087503
- 21. Ferrante RJ, Kubilus JK, Lee J, Ryu H, Beesen A, et al. (2003) Histone deacetylase inhibition by sodium butyrate chemotherapy ameliorates the neurodegenerative phenotype in Huntington's disease mice. J Neurosci 23: 9418–9427.
- 22. Hockly E, Richon VM, Woodman B, Smith DL, Zhou X, et al. (2003) Suberoylanilide hydroxamic acid, a histone deacetylase inhibitor, ameliorates motor deficits in a mouse model of Huntington's disease. Proc Natl Acad Sci U S A 100: 2041–2046. doi: 10.1073/pnas.0437870100
- 23. Gardian G, Browne SE, Choi DK, Klivenyi P, Gregorio J, et al. (2005) Neuroprotective effects of phenylbutyrate in the N171–82Q transgenic mouse model of Huntington's disease. J Biol Chem 280: 556–563. doi: 10.1074/jbc.m410210200
- 24. Jeong H, Then F, Melia TJ Jr, Mazzulli JR, Cui L, et al. (2009) Acetylation targets mutant huntingtin to autophagosomes for degradation. Cell 137: 60–72. doi: 10.1016/j.cell.2009.03.018
- 25. Lehman NL (2009) The ubiquitin proteasome system in neuropathology. Acta Neuropathol 118: 329–347. doi: 10.1007/s00401-009-0560-x
- 26. Sadoul K, Boyault C, Pabion M, Khochbin S (2008) Regulation of protein turnover by acetyltransferases and deacetylases. Biochimie 90: 306–312. doi: 10.1016/j.biochi.2007.06.009
- 27. Spange S, Wagner T, Heinzel T, Kramer OH (2009) Acetylation of non-histone proteins modulates cellular signalling at multiple levels. Int J Biochem Cell Biol 41: 185–198. doi: 10.1016/j.biocel.2008.08.027
- 28. Barrett RM, Wood MA (2008) Beyond transcription factors: the role of chromatin modifying enzymes in regulating transcription required for memory. Learn Mem 15: 460–467. doi: 10.1101/lm.917508
- 29. Levenson JM, O'Riordan KJ, Brown KD, Trinh MA, Molfese DL, et al. (2004) Regulation of histone acetylation during memory formation in the hippocampus. J Biol Chem 279: 40545–40559. doi: 10.1074/jbc.m402229200
- 30. Thomas EA, Coppola G, Desplats PA, Tang B, Soragni E, et al. (2008) The HDAC inhibitor 4b ameliorates the disease phenotype and transcriptional abnormalities in Huntington's disease transgenic mice. Proc Natl Acad Sci U S A 105: 15564–15569. doi: 10.1073/pnas.0804249105
- 31. Mangiarini L, Sathasivam K, Seller M, Cozens B, Harper A, et al. (1996) Exon 1 of the HD gene with an expanded CAG repeat is sufficient to cause a progressive neurological phenotype in transgenic mice. Cell 87: 493–506. doi: 10.1016/s0092-8674(00)81369-0
- 32. Carter RJ, Lione LA, Humby T, Mangiarini L, Mahal A, et al. (1999) Characterization of progressive motor deficits in mice transgenic for the human Huntington's disease mutation. J Neurosci 19: 3248–3257.
- 33. Lione LA, Carter RJ, Hunt MJ, Bates GP, Morton AJ, et al. (1999) Selective discrimination learning impairments in mice expressing the human Huntington's disease mutation. J Neurosci 19: 10428–10437.
- 34. Stack EC, Kubilus JK, Smith K, Cormier K, Del Signore SJ, et al. (2005) Chronology of behavioral symptoms and neuropathological sequela in R6/2 Huntington's disease transgenic mice. J Comp Neurol 490: 354–370. doi: 10.1002/cne.20680
- 35. Vannini A, Volpari C, Filocamo G, Casavola EC, Brunetti M, et al. (2004) Crystal structure of a eukaryotic zinc-dependent histone deacetylase, human HDAC8, complexed with a hydroxamic acid inhibitor. Proc Natl Acad Sci U S A 101: 15064–15069. doi: 10.1073/pnas.0404603101
- 36. Chou CJ, Herman D, Gottesfeld JM (2008) Pimelic diphenylamide 106 is a slow, tight-binding inhibitor of class I histone deacetylases. J Biol Chem 283: 35402–35409. doi: 10.1074/jbc.m807045200
- 37. Bressi JC, Jennings AJ, Skene R, Wu Y, Melkus R, et al. (2010) Exploration of the HDAC2 foot pocket: Synthesis and SAR of substituted N-(2-aminophenyl)benzamides. Bioorg Med Chem Lett 20: 3142–3145. doi: 10.1016/j.bmcl.2010.03.091
- 38. Xu C, Soragni E, Chou CJ, Herman D, Plasterer HL, et al. (2009) Chemical probes identify a role for histone deacetylase 3 in Friedreich's ataxia gene silencing. Chem Biol 16: 980–989. doi: 10.1016/j.chembiol.2009.07.010
- 39. Mielcarek M, Benn CL, Franklin SA, Smith DL, Woodman B, et al.. (2011) SAHA decreases HDAC 2 and 4 levels in vivo and improves molecular phenotypes in the R6/2 mouse model of Huntington’s disease. PLOS ONE: In press.
- 40. Moumne L, Campbell K, Howland D, Ouyang Y, Bates GP (2012) Genetic knock-down of HDAC3 does not modify disease-related phenotypes in a mouse model of Huntington's disease. PLOS ONE 7: e31080. doi: 10.1371/journal.pone.0031080
- 41. Wegener D, Wirsching F, Riester D, Schwienhorst A (2003) A fluorogenic histone deacetylase assay well suited for high-throughput activity screening. Chem Biol 10: 61–68. doi: 10.1016/s1074-5521(02)00305-8
- 42. Bradner JE, West N, Grachan ML, Greenberg EF, Haggarty SJ, et al. (2010) Chemical phylogenetics of histone deacetylases. Nat Chem Biol 6: 238–243. doi: 10.1038/nchembio.313
- 43. Jones P, Altamura S, De Francesco R, Gallinari P, Lahm A, et al. (2008) Probing the elusive catalytic activity of vertebrate class IIa histone deacetylases. Bioorg Med Chem Lett 18: 1814–1819. doi: 10.1016/j.bmcl.2008.02.025
- 44. Muraglia E, Altamura S, Branca D, Cecchetti O, Ferrigno F, et al. (2008) 2-Trifluoroacetylthiophene oxadiazoles as potent and selective class II human histone deacetylase inhibitors. Bioorg Med Chem Lett 18: 6083–6087. doi: 10.1016/j.bmcl.2008.09.076