Copy number variation (CNV) at the 15q11.2 region, which includes a gene that codes for CYFIP1 (cytoplasmic FMR1 interacting protein 1), has been implicated in autism, intellectual disability and additional neuropsychiatric phenotypes. In the current study we studied the function of Cyfip1 in synaptic physiology and behavior, using mice with a disruption of the Cyfip1 gene.
We observed that in Cyfip1 heterozygous mice metabotropic glutamate receptor (mGluR)-dependent long-term depression (LTD) induced by paired-pulse low frequency stimulation (PP-LFS) was significantly increased in comparison to wildtype mice. In addition, mGluR-LTD was not affected in the presence of protein synthesis inhibitor in the Cyfip1 heterozygous mice, while the same treatment inhibited LTD in wildtype littermate controls. mGluR-agonist (RS)-3,5-dihydroxyphenylglycine (DHPG)-induced LTD was also significantly increased in hippocampal slices from Cyfip1 heterozygous mice and again showed independence from protein synthesis only in the heterozygous animals. Furthermore, we observed that the mammalian Target of Rapamycin (mTOR) inhibitor rapamycin was only effective at reducing mGluR-LTD in wildtype animals. Behaviorally, Cyfip1 heterozygous mice showed enhanced extinction of inhibitory avoidance. Application of both mGluR5 and mGluR1 antagonist to slices from Cyfip1 heterozygous mice reversed the increase in DHPG-induced LTD in these mice.
These results demonstrate that haploinsufficiency of Cyfip1 mimics key aspects of the phenotype of Fmr1 knockout mice and are consistent with the hypothesis that these effects are mediated by interaction of Cyfip1 and Fmrp in regulating activity-dependent translation. The data provide support for a model where CYFIP1 haploinsufficiency in patients results in intermediate phenotypes increasing risk for neuropsychiatric disorders.
Citation: Bozdagi O, Sakurai T, Dorr N, Pilorge M, Takahashi N, et al. (2012) Haploinsufficiency of Cyfip1 Produces Fragile X-Like Phenotypes in Mice. PLoS ONE 7(8): e42422. doi:10.1371/journal.pone.0042422
Editor: Ted M. Dawson, Johns Hopkins, United States of America
Received: December 7, 2010; Accepted: July 9, 2012; Published: August 10, 2012
Copyright: © Bozdagi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the Seaver Foundation. OB and TS are Seaver Fellows. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: OB, TS, and JDB have submitted a patent on this work (“Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists”; No. '61/255,340; File date: 10/27/2009). This does not alter the authors’ adherence to all the PLoS ONE policies on sharing data and materials.
CNVs in the 15q11.2 (BP1–BP2) region represent replicated risk factors for schizophrenia, epilepsy, intellectual disability, developmental delay, and autism , , , , . For example, in two large studies of schizophrenia recurrent CNVs were identified which involved the 15q11.2 region that were associated with increased risk , . The CNV includes a minimal 0.3 Mb region that encompasses five refseq genes (TUBGCP5, CYFIP1, NIPA2, NIPA1, and WHAMML1, see ) and increases risk for schizophrenia by 2–4 fold. This interval has already been of interest in psychiatric disorders because of its involvement in autism spectrum disorders (ASD) involving duplications of 15q11-q13 and Prader-Willi and Angelman syndromes . Type I deletions of Prader-Willi and Angelman syndromes, which include this interval, have been associated with more severe manifestations, as compared to deletions (type II) that do not include this interval, including greater severity of ASD features , , , . Several studies indicate that this same region increases risk for developmental disorders including ASD, likely in the presence of other genetic risk factors , , , . In the largest study to date, involving over 15,000 patient samples, deletion of the 15q11.2 region was very strongly associated with developmental delays including ASD, with incomplete penetrance .
CYFIP1, a gene in this region, codes for a protein that binds the Fragile X protein FMRP, and is therefore a candidate gene for psychiatric phenotypes. Fragile X syndrome (FXS) is the most common inherited form of intellectual disability, is frequently associated with co-morbid ASD, and is most commonly caused by transcriptional silencing of the FMR1 gene . The gene product of FMR1 is fragile X mental retardation protein (FMRP). CYFIP1 was identified as one of two highly conserved cytoplasmic FMR1 interacting proteins . In addition to its interaction with FMRP, CYFIP1 was also shown to interact with the small GTPase Rac1 . Both FMRP and Rac1 have been shown to be involved in neuronal and synaptic function. FMRP is regulated in response to mGluR activation , ,  and Fmr1 knockout mice show increased mGluR-dependent LTD (mGluR-LTD) . FMRP has been shown to be involved in negatively regulating translation in synapses and this negative regulation can be removed as a result of neuronal activity . Disruption of FMRP in FXS hence results in increased translation of synaptic proteins, which in turn can lead to down-regulation of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, and lead to disruption of normal synaptic plasticity. Enhanced mGluR-LTD in the absence of FMRP has given rise to the mGluR hypothesis of FXS; higher hippocampal (mGluR-dependent) LTD in Fmr1 knockout mice no longer shows a requirement for protein synthesis (as the normal control on protein synthesis mediated by FMRP is lost). Recently, it has been shown that CYFIP1 can directly bind to the translation initiation factor eIF4E and, like FMRP, negatively regulates FMRP target mRNAs . Stimulation of neurons was shown to cause the dissociation of CYFIP1 from eIF4E at synapses, resulting in protein synthesis, thus providing a mechanism for the activity-dependent regulation of translation seen with FMRP and CYFIP1. The association of CYFIP1 with FMRP, the latter a protein directly implicated in neurodevelopment and psychiatric disorders, makes CYFIP1 a very compelling candidate for an important role in the phenotypes associated with CNVs in the 15q11.2 region , , , . At the same time haploinsufficiency of any or all of the additional genes in the region may also have an additional contributory role.
In order to characterize the functions of CYFIP1 as related to neuropsychiatric phenotypes we developed mice with a disruption in Cyfip1. Similar to what has been observed in Fmr1 knockout mice, Cyfip1 heterozygous mice expressed enhanced mGluR-LTD, which was resistant to protein synthesis inhibition. LTD in the Cyfip1 heterozygous mice was insensitive to inhibition of mTOR pathway, in contrast to what was observed in wildtype littermates. Furthermore, Cyfip1 heterozygous mice exhibited enhanced extinction of inhibitory avoidance. Finally, exposure of hippocampal slices to mGluR antagonists reversed the increase in mGluR-LTD in Cyfip1 knockout mice. The remarkable overlap in phenotype between Fmr1 knockout mice and Cyfip1 heterozygous mice is consistent with a model in which CYFIP1 haploinsufficiency results in intermediate phenotypes increasing risk for neuropsychiatric disorders.
Generation and Validation of Mice with a Disruption in the Cyfip1 Gene
To directly test whether Cyfip1 expression is required in development and synaptic function, we made use of gene-trapped embryonic stem (ES) cells to develop mice with a disruption in Cyfip1 (Fig. 1A). Despite numerous attempts, we never recovered mice with a disruption of both copies of Cyfip1 (knockouts), and even at embryonic days 4 and 5 there was no evidence for knockout embryos. These results indicate that Cyfip1 is necessary for early embryonic development. We did obtain mice with a disruption of a single copy of Cyfip1 (heterozygotes) at expected ratios, when crossing heterozygotes inter se or crossing heterozygotes with wildtype animals.
(A) The genomic structure of Cyfip1 is shown to scale with larger horizontal boxes representing exons, and the first (ATG) and last (Stop) coding exons indicated. The diagram shows the site of the gene-trap insertion (identified as an LTR-flanked Trapping casette) in intron 1 (5′ to the first coding exon), in order to generate mice with a disruption of the Cyfip1 gene. (B) Synaptoneurosome preparations from wildtype (Wt) and Cyfip1 heterozygous (Het) mice were subjected to quantitative immunoblotting with an antibody to Cyfip1, with actin as a reference protein. The migration of molecular weight markers is shown on the left (in kDa). (C) Brain mRNA from wildtype (black bars) and Cyfip1 heterozygous (white bars) mice were subjected to qPCR for the indicated genes. (D) Quantification of Cyfip1 and Fmrp by immunoblotting of extracts from wildtype (black bars) and heterozygous (white bars) mice. *, P<0.05; **, P = 0.004.
To confirm reduced expression of Cyfip1, we made use of immunoblotting using cortices from 4 weeks old mice and observed a ~40% reduction in expression of Cyfip1 in heterozygotes, as compared to wildtype littermates (Figs. 1B, 1D). We also measured gene expression by quantitative PCR (qPCR), and found that Cyfip1 mRNA is reduced by 50% (Fig. 1C). There was no compensatory change in Cyfip2, nor was there any change in expression from other genes that flank Cyfip1 (Tubgpc5, Nipa1, Nipa2), which might have been affected by the gene trap (Fig. 1C). Furthermore, we did not detect potential compensatory changes in levels of Fmrp (Fig. 1D).
Developmental milestones, assessed by physical development, motor development, and reflexes, were normal in Cyfip1 heterozygote mice. The assessments included monitoring the development of cliff avoidance, forelimb placing, vibrissa placing, visual placing, auditory startle, tactile startle and toe pinch. Fear-induced freezing was also measured. There was no difference between wildtype and Cyfip1 heterozygous mice in any of these measures. Altogether, developmental milestones in the heterozygotes were within normal limits, making them appropriate for further, detailed studies.
Increased mGluR-dependent Long-term Depression in Cyfip1 Heterozygotes
There have been extensive studies of the effects of loss of FMRP on electrophysiological properties in the hippocampus, particularly as pertains to synaptic plasticity. The association of FMRP with CYFIP1 supported similar analyses in the Cyfip1 heterozygotes. We first examined basal synaptic transmission in the Schaffer collateral pathway in hippocampal slices, determined by input/output analyses and paired-pulse facilitation, to study the effect of Cyfip1 on presynaptic release probability and short-term synaptic plasticity. We found no significant differences between genotypes in input/output function (Fig. 2A) or in paired-pulse ratio over the test interpulse interval of 50 ms (1.26±0.06 and 1.27±0.04, in wildtype and heterozygous mice, respectively, using 6 mice per genotype, p = 0.4, Student’s t-test) (Fig. 2B). Together these results indicate that neither basal synaptic transmission nor the efficiency of neurotransmitter release is altered in Cyfip1 heterozygotes.
(A) Hippocampal slices from 4–6 weeks old wildtype (WT) or Cyfip1 heterozygous (Het) mice were analyzed for baseline synaptic properties, determined by input/output function, representing the relationship between stimulus intensity and the size of the field EPSP slope. (B) Paired-pulse facilitation in the Schaffer collateral-commissural pathway is not different between genotypes over the test interpulse interval of 50 ms. (C) HFS-induced LTP was not significantly different between wildtype (WT) or Cyfip1 heterozygous (Het) mice. (D) PP-LFS-induced LTD in Cyfip1 heterozygous mice was significantly increased. Inset: Representative EPSP traces recorded before stimulation (arrow) or 60 min after stimulation in wildtype and heterozygous animals (scale: 10 ms and 0.5 mV).
Long-term potentiation (LTP) is an important measure of synaptic plasticity. We induced LTP by high frequency stimulation consisting of four trains of 100 Hz for 1 second, separated by 5 minutes, a protocol which induces a protein synthesis-dependent form of LTP (Fig. 2C). The induction and maintenance of LTP were not significantly different between hippocampal slices from wildtype and heterozygous mice over the 120 min time course after tetanic stimulation (average percentage of baseline 120 min after tetanus: wildtype, 168.6±9.4%; heterozygote, 159.8±11.4%; n = 6 slices, 4 mice per group; F (1,10) = 1.1 p = 0.23). In another set of experiments we further tested LTP induced by threshold levels of theta-burst afferent stimulation, which also did not demonstrate differences between genotypes (average percentage of baseline 30 min after tetanus: wildtype, 122.1±4.2%; heterozygote, 116.8±8.9%; n = 6 slices, 4 mice per group; F (1,10) = 2.06 p = 0.12). Finally early phase LTP (E-LTP) was induced with 100 Hz tetanic stimulation, for 1 second. There were no significant differences amongst the two genotypes with this stimulation paradigm (average percentage of baseline 60 min after tetanus: wildtype, 131.7±9%; heterozygote, 130.0±8.1%; F (1,9) = 0.9 p = 0.41).
Metabotropic receptor-dependent long-term depression (mGluR-LTD) is another important measure of synaptic plasticity and one that has been shown to be altered the absence of FMRP. To examine the role of Cyfip1 in mGluR-LTD we recorded field EPSPs at Schaffer collateral-CA1 synapses in acute hippocampal slices. In slices derived from wildtype animals, LTD induced by paired pulse-low frequency stimulation (PP-LFS) resulted in a reduction in field EPSP slope to 79.9±2.4% of baseline, while in slices derived from heterozygous animals the magnitude of the depression was significantly increased, to 67.8±8% of baseline (n = 9 slices, 6 mice for wildtype and 10 slices, 6 mice for heterozygotes; F (1,17) = 5.4 p = 0.03) (Fig. 2D). We then tested the role of Cyfip1 in NMDAR-dependent LTD using 15 min LFS consisting of 900 pulses at 1 Hz (data not shown). There were no significant differences between genotypes with this stimulation paradigm (wildtype, 82.6±3.6%; heterozygote, 79.9±2%; measured 60 min after LFS; n = 7 slices, 4 mice for wildtype and 7 slices, 3 mice for heterozygotes; F (1,12) = 2.15 p = 0.1).
mGluR-LTD is Independent of Protein Synthesis in Cyfip1 Heterozygotes
One of the most striking abnormalities in experimental synaptic plasticity observed in the absence of FMRP is that mGluR-LTD is independent of protein synthesis in these animals. As the Cyfip1 heterozygotes have enhanced LTD, similar to what has been observed in the absence of FMRP, we next compared mGuR-LTD in the absence of presence of the protein synthesis inhibitor cycloheximide. PP-LFS-induced LTD was not affected by the addition of cycloheximide (60 µM) in the Cyfip1 heterozygous animals, while the same treatment inhibited LTD in the wildtype littermate controls (Figs. 3A, 3B). In the presence of cycloheximide, fEPSP slope in slices from wildtype animals was at 98.05±2.5% of baseline values at 60 minutes after PP-LFS, while in sliced from heterozygous animals the slope was at 68.49±8.9% of baseline (n = 8 slices, 6 mice per group, F (1,17) = 11.7 p = 0.01).
(A, B) LTD induced by PP-LFS in wild type (A) or Cyfip1 heterozygous (B) mice, in the absence (o) or presence (•) of the protein synthesis inhibitor cycloheximide (60 µM). The effect of cycloheximide is significantly different across genotypes. (C, D) LTP induced by HFS in wildtype (C) or Cyfip1 heterozygous (D) mice, in the absence (o) or presence (•) of cycloheximide. In all panels, the large arrow indicates onset of stimulation. (E, F) Rapamycin blocks LTP induced by HFS in wildtype (E) and Cyfip1 heterozygous (F) mice, as shown by incubating slices in the absence (o) or presence (•) of the mTOR inhibitor rapamycin (20 nM). Onset of stimulation is indicated by an arrow.
In contrast to the results with LTD, we observed that the inhibition of protein synthesis-dependent LTP with cycloheximide did not differ between genotypes (Figs. 3C, 3D) (measuring at 120 minutes after tetanus, field EPSP slope was 168±9.4% of baseline for wildtype slices, 99±8% for wildtype slices in the presence of cycloheximide, 156.7±11.4% for heterozygous slices, and 100.8±8.2% for heterozygous slices in the presence of cycloheximide). Treatment of the slices with mTOR inhibitor rapamycin also reduced LTP in studies with both the wildtype and heterozygous mice (Figs. 3E, 3F).
Increased Chemically Induced mGluR-LTD in Cyfip1 Heterozygous Mice
The observation that tetanic-stimulation induced LTD, but not LTP, was independent of protein synthesis with a 50% reduction in levels of Cyfip1 was particularly intriguing. To further confirm this finding, we made use of another method to induce LTD, using DHPG, which is an agonist that activates group I mGluRs. This treatment induced a depression in synaptic transmission to 81.2±3% of baseline at 30 min of application in wildtype mice (Fig. 4A). In heterozygous mice, this treatment led to significantly increased LTD (70.5±6% of baseline) as compared to wildtype animals (Fig. 4A, n = 7 slices, 4 mice for wildtype and 8 slices, 4 mice for heterozygotes; F (1,13) = 6.15 p = 0.023). As before, the addition of 60 µM cycloheximide reduced LTD in wildtype, but not Cyfip1 heterozygous mice (F (1,12) = 1.15 p = 0.2; Figs. 4B, 4C). We next tested mTOR dependency of mGluR-LTD by bath-applying 20 nM rapamycin starting 15 min before DHPG application and continuing through the end of the experiment. We observed that rapamycin treatment reduced mGluR-LTD in wildtype, but not Cyfip1 heterozygous littermates (F (1,11) = 1.4, p = 0.24; Figs. 4D, 4E).
(A) LTD was induced by DHPG (50 µM for 5 minutes, indicated by the short horizontal bar) in hippocampal slices from wildtype (Wt) and Cyfip1 heterozygous (Het) mice. LTD is significantly enhanced in the heterozygotes as compared to wildtype. Inset: Representative EPSP traces recorded before (arrow) or 40 min after DHPG in wildtype and heterozygous animals (scale: 10 ms and 0.5 mV). (B, C) LTD was induced with DHPG in wildtype (B) or Cyfip1 heterozygous (C) mice, in the absence (o) or presence (•) of cycloheximide (Cyclohex, 60 µM, indicated by the long horizontal bar). Cycloheximide significantly inhibited LTD in slices from wildtype but not heterozygous animals. (D, E) LTD was induced by DHPG (50 µM, indicated by the short horizontal bar) in hippocampal slices from wildtype (D) or Cyfip1 heterozygous (E) mice, in the absence (o) or presence (•) of rapamycin (20 nM, indicated by the long horizontal bar). (F) LTD was induced by DHPG (50 µM, indicated by the short horizontal bar) in hippocampal slices from wildtype (o) or Cyfip1 heterozygous (•) mice, the latter in the absence (•) or presence (▪) of both MPEP (10 µM) and LY367385 (indicated by the long horizontal bar). Application of the mGluR1 and mGluR5 antagonists decreased the magnitude of DHPG-induced-LTD in heterozygotes.
Reversal of Enhanced LTD in Cyfip1 Heterozygotes by mGluR Antagonists
Because mGluR activation is essential for mGluR-LTD induction, we examined the effect of mGluR blockade on DHPG-induced LTD. Slices were incubated in both mGluR1 (LY367385, 100 µM) and mGluR5 (MPEP, 10 µM) antagonists. Bath application of both compounds to slices derived from Cyfip1 heterozygotes significantly decreased the magnitude of LTD in these slices to control levels (wildtype: 81.2±3% of baseline at 30 minutes of DHPG application, Cyfip1 heterozygotes, 70.5±6% of baseline, Cyfip1 heterozygotes in the presence of MPEP and LY367385, 83±5.7% of baseline, F(2,22) = 4.18, p = 0.03) (Fig. 4F).
Enhanced Extinction of Inhibitory Avoidance in Cyfip1 Heterozygotes
Anxiety and abnormal social behavior are significant characteristics of several neuropsychiatric disorders in humans. We therefore first examined open field analysis, light dark transition and elevated zero maze in Cyfip1 heterozygote mice and wildtype littermates and did not observe any differences as a function of genotype (Table S1).
To examine the role of Cyfip1 in hippocampal dependent learning, we used a Y-maze (to detect alterations in working memory; data not shown) and Morris Water Maze (Fig. 5A) (to detect spatial learning and memory ability), which requires both an intact hippocampus and amygdala , , in Cyfip1 heterozygote and wildtype mice. There were no differences between genotypes in these tests. Furthermore, we used a contextual fear paradigm and did not detect any changes between genotypes (Fig. 5B). All of these findings are similar to behavioral findings in Fmr1 knockout mice. It has been shown, however, that Fmr1 knockout mice show more rapid extinction in inhibitory avoidance testing , so it was of interest to perform inhibitory avoidance testing with the same paradigm. As has been described with Fmr1 knockout mice, we observed a more rapid extinction timeline in the heterozygotes (at extinction 2, t-test, P = 0.027; Fig. 5C).
(A) Mice were tested using the Morris Water Maze. Time (s) to travel to the target platform was not significantly different between genotypes. (B) Mice were tested for fear conditioning, with mice receiving shocks at 120 and 180 seconds during training. Testing was performed 24 hours later, in the same test chamber, without footshock. (C) Inhibitory avoidance was measured by latency to enter the dark side of the box associated with prior shock. Extinction of inhibitory avoidance is enhanced in the heterozygotes. The lower panel shows the experimental design. WT, wildtype mice; Het, heterozygous mice; acq, acquisition; ext, extinction; IA, inhibitory avoidance. *, P = 0.027.
Based on the evidence for 15q11.2 deletion and duplications in psychiatric phenotypes and the presence of the FMRP-binding protein CYFIP1 in this interval, we carried out functional analyses of Cyfip1. Our data indicate that Cyfip1 heterozygous mice exhibit reduced expression of Cyfip1, which is in turn associated with an enhancement of hippocampal mGluR-LTD, without affecting hippocampal LTP or even basal synaptic processes.
Protein synthesis is required for several different forms of synaptic plasticity, and control of protein synthesis is a critical mechanism for modulating long-term changes in neural circuits and resultant behavioral changes . Protein synthesis is required for mGluR-LTD . FMRP is an important regulator of translation in the brain and recently is has been shown that FMRP represses translation initiation (the rate limiting step in translation and hence an important target for regulation) via interaction with CYFIP1 . These authors provide compelling evidence that CYFIP1 functions like other eukaryotic initiation factor (eIF) 4E-binding proteins (4E-BP), competing with eIF4G binding to eIF4E. Disrupting the eIF4E-eIF4G interaction inhibits translation as the bridge between the mRNA and the ribosomal pre-initiation complex is lost . For canonical 4E-BP proteins, and perhaps for eIF4G, phosphorylation by an activated mTOR complex reverses the blockade on translation , a mechanism that may also occur with CYFIP1 . In some studies, loss of FMRP leads to an increase in expression of target genes such as CamkII in synaptoneurosome, in steady state , . We did not observe an increase in CamkII expression in our studies (data not shown). However as these effects are often subtle and difficult to measure, we cannot exclude an effect in basal levels of Fmrp-Cyfip1 targets in the absence of stimulation.
Here, we showed that incubation of slices with the mRNA translation inhibitor cycloheximide or the mTOR inhibitor rapamycin blocked mGluR-LTD in wildtype but not in heterozygous mice. The mTOR pathway plays a role in translation initiation and generation of translation elongation factors. The application of rapamycin prevents the phosphorylation of the translational regulator 4E-BP1 by mTOR. In its unphosphorylated state, 4E-BP1 remains bound to the translation initiation factor, eukaryotic initiation factor 4E (eIF4E), and the initiation of translation are inhibited.
In our experiments we observed that mGluR-LTD in Cyfip1 heterozygous mice is insensitive to inhibition of protein synthesis demonstrating that the normal control of activity-regulated protein synthesis is lost in these mice. Previous work has demonstrated that mGluR-LTD was enhanced in Fmr1 knockout mice and was unaffected by the presence of protein synthesis inhibitors . The loss of the protein synthesis dependency of LTD in both these examples likely arises from a common mechanism, in which reduction of the levels of either member of a Cyfip1/Fmrp complex disrupts the baseline suppression of local translation in the synapse. Our findings also indicate that while mTOR plays a role in mGluR-LTD in wildtype mice, this mechanism is altered in Cyfip1 heterozygous mice as we observed that rapamycin only reduced mGluR-LTD in studies with the wildtype animals. Altogether, our results support dysregulation of protein synthesis in the synapse and are consistent with studies in Cyfip1 heterozygotes .
In our study, LTP induced by high frequency stimulation or threshold theta burst stimulation was unaltered in area CA1 in Cyfip1 heterozygotes. LTP in CA1 induced by high frequency stimulation is also known to be unaffected in Fmr1 knockout mice , , however, LTP elicited by threshold theta burst afferent stimulation is impaired in young adult Fmr1 knockout mice  which is reversed by BDNF perfusion. Whether this is an age-specific effect or a difference between the two models remains to be determined.
How the alterations in hippocampal mGluR-LTD could contribute to the cognitive deficits is not known. Behavioral deficits in Fmr1 knockout animals are typically quite subtle, however, extinction in a one-trial inhibitory avoidance paradigm, shown to be dependent on protein synthesis in the hippocampus, is enhance in Fmr1 knockout animals . Behavioral characterization of Cyfip1 heterozygotes in our study also showed typical behaviours in many assays, including those assessing anxiety, social behaviours, and cognition, but more rapid extinction in inhibitory avoidance testing, similar to what has been described for Fmr1 knockouts, supports a shared mechanism. However, because there is an effect of genetic background in some of the behavioural phenotypes in Fmr1 knockout animals ,  (see Table S2), the effect of genetic background must be considered in the modest behavioral changes in Cyfip1-deficient mice.
The development of a mouse model with a loss of a functional copy of Cyfip1 provides an important resource to understand the role of this gene in psychiatric and neurological illnesses and in screening potential therapies. One potential intervention appears to be the targeting of mGluR with antagonists. It is of interest that use of two antagonists together produced a reversal of enhanced mGluR-LTD in Cyfip1 heterozygous mice in our experiments, suggesting that a combined approach would be beneficial in both 15q11.2 CNV patients but also in FXS.
In summary, mice lacking one functional copy of Cyfip1 show enhanced mGluR-LTD that is independent of protein synthesis and reversed by mGluR antagonists, as well as more rapid extinction in an inhibitory avoidance paradigm. These findings are identical to those observed in Fmr1 knockout mice . Note that our expression studies exclude an indirect effect of Cyfip1 depletion mediated through reduced expression of Fmrp, as Fmrp expression was normal. These observations indicate that gene dosage abnormalities of CYFIP1 can alter synaptic plasticity and function, and supports shared mechanisms between FXS and phenotypes associated with the loss of a functional copy of CYFIP1. The partial loss of CYFIP1 expression, when considered together with the restricted regional expression of CYFIP1, especially as compared to the ubiquitous expression of CYFIP2 and FMRP, could explain why the behavioural consequences of CYFIP1-deficiency are not necessarily as severe as what is observed with loss of FMRP. Our studies are consistent with a model in which haploinsufficiency of CYFIP1 leads to an intermediate phenotype that, in the context of additional factors, can results in divers neuropsychiatric conditions.
Materials and Methods
Generation of Mice with a Disruption of the Cyfip1 Gene
All animal procedures were approved by the IACUC at Mount Sinai School of Medicine and the Bronx VA Medical Center.
Mice were developed from an Omnibank (Lexicon) embryonic stem (ES) cell line, with Cyfip1 targeted by mutagenesis with a gene trap insertional vector. Briefly, we identified an ES clone that has a trapping cassette inserted into intron 1 of the Cyfip1 gene (note that the start ATG is in exon 2). A mouse line was established from the ES cells in the 129SvEvBrd strain, subsequently backcrossed to C57Bl/6Tac.
Analysis of Developmental Milestones
All testing was done blind to genotype. We made use of a systematic approach to assess development in the mice, following our prior approach , as well as a recent detailed protocol . We tested cohorts beginning at 3 days in 3-day increments until the animals were 27 days old; the tester kept track of individual pups by marking their tails with a non-toxic, low odor marker. A total of 5 litters were assessed. Each pup was observed for physical development and tested on a number of reflexes. To assess physical development, body weight was measured, while hallmarks including fur development, incisor eruption, eye opening and detachment of pinnae were observed and noted. Motor development and reflexes were monitored by appearance and/or disappearance of the righting reflex, crossed extensor reflex, and grasp reflex, and by performance in negative geotaxis, level screen test, vertical screen test, and bar holding, drawn from standard SHIRPA descriptions . Sensory and motor coordination was monitored by the appearance of cliff avoidance, forelimb placing, vibrissa placing, visual placing, auditory startle, tactile startle, and toe pinch. Fear-induced freezing was measured after the pup was placed in a 100-ml beaker and dropped by inverting the beaker.
We prepared cohorts of 28 male animals (13 wildtype and 15 heterozygotes) from 6 litters from wild type x heterozygote matings. Behavioral studies were conducted at the Rat and Mouse Phenotyping Shared Research Facility at Mount Sinai School of Medicine. Mice were transferred to the Facility at 2 months of age, acclimated for 2 months, and went through a test battery starting at 4 months of age. Most procedures have been described previously , . The order of behavioral testing was general observation, open-field, light dark transition, elevated zero maze, social interactions, Y-maze, Morris Water Maze, conditioned fear testing, inhibitory avoidance, and PPI. Morris Water Maze trials were run in a 48" plastic pool. On the first day of the 2-week procedure, test animals were habituated to the pool in a 5 min trial with the platform visible and accessible, extending just out of the water. Test animals were placed in the quadrant opposite the platform, and, as on all subsequent trials, facing into the wall. In the subsequent 8 days of training trials each subject received 4 1-minute trials per day, 10–15 minutes apart. Training days fell in two groups of 4 consecutive days, 2 days apart. In all training days, subjects began one trial in each quadrant, counterclockwise from a starting quadrant that also shifted clockwise one step each day. Each subject’s total elapsed time to find the platform was recorded, and the trial ended if the subject remained on the platform for 5 seconds or more. On the final day, the 9th (probe) day, the platform was removed entirely and subjects given a single 1 min trial starting from the quadrant opposite the original platform location. In this probe trial, latency to reach the target square, time spent in the target quadrant, and time spent in the target square were quantified along with time spent in all other quadrants and corresponding target areas.
Inhibitory avoidance was performed following the protocol published previously  except we used longer cut off times (180 s instead of 120 s) during our initial training phase. We tested at 6 hrs, 24 hours and 48 hours after initial training. We used an inhibitory avoidance box from San Diego Instruments. For training, subjects spend 30 s in dark chamber and were then moved to the start box in the light chamber for 90 s of habituation (gate closed). When the gate opened the light remained on and latency to cross through to dark side was measured (baseline). Once the subject crossed into dark chamber, the gate was closed and the subject receives 2 s of 0.5 mA footshock. After 15 s, the subject was returned to its home cage. Animals with baseline cross-through latencies greater than 180 s were excluded. Six hours later, subjects were tested on retention. After 90 s in light side with gate closed, the gate was opened, and cross-through latency was recorded (with a 540 s cutoff). For the extinction phase subjects were allowed to freely explore the dark chamber for 200 s, with no footshock, before being returned to their home cages. 24 hours after training, post-extinction 1, was carried out in a manner identical to the retention, and, again at 48 hours after training, post-extinction 2 was carried out, also identical to retention, minus 200 s exploration after cross-through.
We prepared synaptoneurosomes from whole cortex of animals at 4 weeks of age for protein analysis , using 4 pairs of heterozygotes and littermate controls for the analyses. Equal amount of proteins were subjected to SDS PAGE, followed by quantitative immunoblotting using a Li-COR system (Li-COR). Images were quantitated by Image-J and the intensity of bands normalized to a reference actin signal for each lane. Antibodies used were anti-Sra-1 (Synaptic systems), anti-Fmrp (Millipore), anti-CamKII (Millipore), and anti-actin (Sigma). The anti-Sra-1 antibody gives only one band in brain extracts as shown in Figure 1.
Quantitative PCR Analysis
Quantitative PCR (qPCR) using the Universal Probe Library system (Roche) was performed as described previously . We designed primers with ProbeFinder (Roche), making use of multiple reference genes for normalization, with data analysis carried out with qBase software. Control genes used were Actb, Gusb, 18S rRNA, and Rpl. Six wild type and six heterozygous male animals (5 weeks old) were used. RNA was prepared from dissected prefrontal cortex using RNAeasy kit (Qiagen), and used for making total cDNA using random primers, and 25 ng of total cDNA was used for the qPCR.
Hippocampal Slice Electrophysiology
Hippocampal slices (350 µm) were prepared from 4–6 week old heterozygous mice and their wildtype littermate controls. Slices were perfused with Ringer’s solution containing (in mM): NaCl, 125.0; KCl, 2.5; MgSO4, 1.3; NaH2PO4, 1.0; NaHCO3, 26.2; CaCl2, 2.5; glucose, 11.0. The Ringer’s solution was bubbled with 95% O2/5% CO2, at 32°C, during extracellular recordings (electrode solution: 3 M NaCl). Slices were maintained for 1 hr prior to establishment of a baseline of field excitatory postsynaptic potentials (fEPSPs) recorded from stratum radiatum in area CA1, evoked by stimulation of the Schaffer collateral-commissural afferents (100 µs pulses every 30 s) with bipolar tungsten electrodes placed into area CA3 . Test stimulus intensity was adjusted to obtain fEPSPs with amplitudes that were one-half of the maximal response. The EPSP initial slope (mV/ms) was determined from the average waveform of four consecutive responses.
Cycloheximide (60 µM, Sigma), (S)-3,5-dihydroxyphenylglycine (DHPG, 50 µM, Sigma), 2-methyl-6-phenylethynyl-pyridine (MPEP, 10 µM, Tocris), LY367385 (100 µM, Tocris), or rapamycin (20 nM, Enzo Life Sciences) were bath-applied for durations indicated in the figure legends. All experiments were performed in the presence of 100 µM 2-amino-5-phosphopentanoic acid (AP5).
Paired-pulse responses were measured with an interstimulus interval (ISI) of 50 ms, and were expressed as the ratio of the average responses to the second stimulation pulse (FP2) to the first stimulation pulse (FP1). Long-term potentiation (LTP) was induced by either a high-frequency stimulus (four trains of 100 Hz, 1 s stimulation separated by 5 min), threshold levels of theta-burst stimulation (TBS) (5 bursts of four pulses at 100 Hz separated by 200 ms, , or a single 100 Hz stimulation. To induce an mGluR-dependent long-term depression (LTD), Schaffer collaterals were stimulated by a paired-pulse low-frequency stimulation (PP-LFS, 1 Hz for 20 min; 50 ms interstimulus interval . DHPG-induced LTD was also used where indicated.
Data are expressed as mean ± SD, and statistical analyses were performed using either a two-way repeated-measures ANOVA, ranged from post-LTP or LTD-inducing stimuli onward until the end of recording, or Student’s t-test, where P<0.05 was considered significant. N’s indicate number of slices (1–3 slices from 3–6 mice per group).
Anxiety-related behavioral measures in Cyfip1 heterozygous mice and wildtype littermates. In the measures for open field, light dark transition and elevated zero maze tests, there were no differences between wildtype and Cyfip1 heterozygous animals.
Comparison of behavioral phenotypes from Cyfip1 heterozygous mice and Fmr1 knockout mice on two different backgrounds. Results of our behavioural assays in Cyfip1 heterozygous mice are compared to Fmr1 knockout mice, based on published data in Fmr1 knockout mice on different backgrounds.
Conceived and designed the experiments: OB TS JDB. Performed the experiments: OB TS ND MP NT. Analyzed the data: OB TS ND MP NT. Contributed reagents/materials/analysis tools: OB TS ND MP NT. Wrote the paper: OB TS JDB.
- 1. Kirov G (2010) The role of copy number variation in schizophrenia. Expert Rev. Neurother. 10 (1): 25–32. doi: 10.1586/ern.09.133
- 2. Tam GW, van de Lagemaat LN, Redon R, Strathdee KE, Croning MD, et al. (2010) Confirmed rare copy number variants implicate novel genes in schizophrenia. Biochem Soc Trans. 38: 445–451. doi: 10.1042/bst0380445
- 3. van der Zwaag B, Staal WG, Hochstenbach R, Poot M, Spierenburg HA, et al. (2010) A co-segregating microduplication of chromosome 15q11.2 pinpoints two risk genes for autism spectrum disorder. Am J Med Genet B Neuropsychiatr Genet. 153B(4): 960–6. doi: 10.1002/ajmg.b.31055
- 4. von der Lippe C, Rustad C, Heimdal K, Rødningen OK (2011) 15q11.2 microdeletion - seven new patients with delayed development and/or behavioural problems. Eur J Med Genet. 54(3): 357–60. doi: 10.1016/j.ejmg.2010.12.008
- 5. Burnside RD, Pasion R, Mikhail FM, Carroll AJ, Robin NH, et al. (2011) Microdeletion/microduplication of proximal 15q11.2 between BP1 and BP2: a susceptibility region for neurological dysfunction including developmental and language delay. Hum Genet. 130 (4): 517–28. doi: 10.1007/s00439-011-0970-4
- 6. Stefansson H, Rujescu D, Cichon S, Pietiläinen OP, Ingason A, et al. (2008) Large recurrent microdeletions associated with schizophrenia. Nature 455 7210: 232–236. doi: 10.1038/nature07229
- 7. Kirov G, Grozeva D, Norton N, Ivanov D, Mantripragada KK, et al. (2009) Support for the involvement of large copy number variants in the pathogenesis of schizophrenia. Hum Mol Genet. 18 8: 1497–1503. doi: 10.1093/hmg/ddp043
- 8. Chai JH, Locke DP, Greally JM, Knoll JH, Ohta T, et al. (2003) Identification of four highly conserved genes between breakpoint hotspots BP1 and BP2 of the Prader-Willi/Angelman syndromes deletion region that have undergone evolutionary transposition mediated by flanking duplicons. Am J Hum Genet. 73 4: 898–925. doi: 10.1086/378816
- 9. Horsthemke B, Wagstaff J (2008) Mechanisms of imprinting of the Prader-Willi/Angelman region. Am J Med Genet. Part A 146A 16: 2041–2052. doi: 10.1002/ajmg.a.32364
- 10. Bittel DC, Kibiryeva N, Butler MG (2006) Expression of 4 genes between chromosome 15 breakpoints 1 and 2 and behavioral outcomes in Prader-Willi syndrome. Pediatrics 118 4: e1276–1283. doi: 10.1542/peds.2006-0424
- 11. Butler MG, Bittel DC, Kibiryeva N, Talebizadeh Z, Thompson T (2004) Behavioral differences among subjects with Prader-Willi syndrome and type I or type II deletion and maternal disomy. Pediatrics 113, 3 Pt 1: 565–573. doi: 10.1542/peds.113.3.565
- 12. Sahoo T, Peters SU, Madduri NS, Glaze DG, German JR, et al. (2006) Microarray based comparative genomic hybridization testing in deletion bearing patients with Angelman syndrome: genotype-phenotype correlations. J Med Genet. 43 6: 512–516. doi: 10.1136/jmg.2005.036913
- 13. Peters SU, Horowitz L, Barbieri-Welge R, Taylor JL, Hundley RJ (2011) Longitudinal follow-up of autism spectrum features and sensory behaviors in Angelman syndrome by deletion class. J Child Psychol Psychiatry. Aug 10. doi: 10.1111/j.1469-7610.2011.02455.x
- 14. Murthy SK, Nygren AO, El Shakankiry HM, Schouten JP, Al Khayat AI, et al. (2007) Detection of a novel familial deletion of four genes between BP1 and BP2 of the Prader-Willi/Angelman syndrome critical region by oligo-array CGH in a child with neurological disorder and speech impairment. Cytogenet Genome Res. 116: 135–140. doi: 10.1159/000097433
- 15. Doornbos M, Sikkema-Raddatz B, Ruijvenkamp CA, Dijkhuizen T, Bijlsma EK, et al. (2009) Nine patients with a microdeletion 15q11.2 between breakpoints 1 and 2 of the Prader-Willi critical region, possibly associated with behavioural disturbances. Eur J Med Genet. 52 2–3: 108–115. doi: 10.1016/j.ejmg.2009.03.010
- 16. Cooper GM, Coe BP, Girirajan S, Rosenfeld JA, Vu TH, et al. (2011) A copy number variation morbidity map of developmental delay. Nat Genet. Aug 14 43(9): 838–46. doi: 10.1038/ng.909
- 17. Leblond CS, Heinrich J, Delorme R, Proepper C, Betancur C, et al. (2012) Genetic and Functional Analyses of SHANK2 Mutations Suggest a Multiple Hit Model of Autism Spectrum Disorders. PLoS Genet. Feb 8(2): e1002521. doi: 10.1371/journal.pgen.1002521
- 18. Garber KB, Visootsak J, Warren ST (2008) Fragile X syndrome. Eur J Hum Genet. 16 6: 666–672. doi: 10.1038/ejhg.2008.61
- 19. Schenck A, Bardoni B, Moro A, Bagni C, Mandel JL (2001) A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P. Proc Natl Acad Sci U S A. 98 15: 8844–8849. doi: 10.1073/pnas.151231598
- 20. Schenck A, Bardoni B, Langmann C, Harden N, Mandel JL, et al. (2003) CYFIP/Sra-1 controls neuronal connectivity in Drosophila and links the Rac1 GTPase pathway to the fragile X protein. Neuron 38 6: 887–898. doi: 10.1016/s0896-6273(03)00354-4
- 21. Ferrari F, Mercaldo V, Piccoli G, Sala C, Cannata S, et al. (2007) The fragile X mental retardation protein-RNP granules show an mGluR-dependent localization in the post-synaptic spines. Mol Cell Neurosci. 34(3): 343–54. doi: 10.1016/j.mcn.2006.11.015
- 22. Antar LN, Afroz R, Dictenberg JB, Carroll RC, Bassell GJ (2004) Metabotropic glutamate receptor activation regulates fragile x mental retardation protein and FMR1 mRNA localization differentially in dendrites and at synapses. J Neurosci. 17 24(11): 2648–55. doi: 10.1523/jneurosci.0099-04.2004
- 23. Weiler IJ, Spangler CC, Klintsova AY, Grossman AW, Kim SH, et al. (2004) Fragile X mental retardation protein is necessary for neurotransmitter-activated protein translation at synapses. Proc Natl Acad Sci U S A. 14 101(50): 17504–9. doi: 10.1073/pnas.0407533101
- 24. Huber KM, Gallagher SM, Warren ST, Bear MF (2002) Altered synaptic plasticity in a mouse model of fragile X mental retardation. Proc Natl Acad Sci U S A. 2002 May 28 99(11): 7746–50. doi: 10.1073/pnas.122205699
- 25. Napoli I, Mercaldo V, Boyl PP, Eleuteri B, Zalfa F, et al. (2008) The fragile X syndrome protein represses activity-dependent translation through CYFIP1, a new 4E-BP. Cell 134 6: 1042–1054. doi: 10.1016/j.cell.2008.07.031
- 26. Murphy SM, Preble AM, Patel UK, O’Connell KL, Dias DP, et al. (2001) GCP5 and GCP6: two new members of the human gamma-tubulin complex. Mol Biol Cell. 12 11: 3340–3352. doi: 10.1091/mbc.12.11.3340
- 27. James AP, Talbot K (2006) The molecular genetics of non-ALS motor neuron diseases. Biochimica Et Biophysica Acta 1762 11–12: 986–1000. doi: 10.1016/j.bbadis.2006.04.003
- 28. Izumi N, Fumoto K, Izumi S, Kikuchi A (2008) GSK-3beta regulates proper mitotic spindle formation in cooperation with a component of the gamma-tubulin ring complex, GCP5. J Biol Chem. 283 19: 12981–12991. doi: 10.1074/jbc.m710282200
- 29. Botzolakis EJ, Zhao J, Gurba KN, Macdonald RL, Hedera P (2010) The effect of HSP-causing mutations in SPG3A and NIPA1 on the assembly, trafficking, and interaction between atlastin-1 and NIPA1. Mol Cell Neurosci. Sep 22. doi: 10.1016/j.mcn.2010.08.012
- 30. Phillips RG, LeDoux JE (1992) Differential contribution of amygdala and hippocampus to cued and contextual fear conditioning. Behav Neurosci. 106(2): 274–85. doi: 10.1037//0735-7044.106.2.274
- 31. Goosens KA, Maren S (2001) Contextual and auditory fear conditioning are mediated by the lateral, basal, and central amygdaloid nuclei in rats. Learn Mem. 8(3): 148–55. doi: 10.1101/lm.37601
- 32. Dölen G, Osterweil E, Rao BS, Smith GB, Auerbach BD, et al. (2007) Correction of fragile X syndrome in mice. Neuron 56 6: 955–962. doi: 10.1016/j.neuron.2007.12.001
- 33. Costa-Mattioli M, Sossin WS, Klann E, Sonenberg N (2009) Translational control of long-lasting synaptic plasticity and memory. Neuron 61 1: 10–26. doi: 10.1016/j.neuron.2008.10.055
- 34. Hou L, Antion MD, Hu D, Spencer CM, Paylor R, Klann E (2006) Dynamic translational and proteasomal regulation of fragile X mental retardation protein controls mGluR-dependent long-term depression. Neuron 51 (4): 441–54. doi: 10.1016/j.neuron.2006.07.005
- 35. Nosyreva ED, Huber KM (2006) Metabotropic receptor-dependent long-term depression persists in the absence of protein synthesis in the mouse model of fragile X syndrome. J Neurophysiol. 95 5: 3291–3295. doi: 10.1152/jn.01316.2005
- 36. Godfraind JM, Reyniers E, De Boulle K, D’Hooge R, De Deyn PP, et al. (1996) Long-term potentiation in the hippocampus of fragile X knockout mice. Am J Med Genet. 64 2: 246–251. doi: 10.1002/(sici)1096-8628(19960809)64:2<246::aid-ajmg2>3.0.co;2-s
- 37. Paradee W, Melikian HE, Rasmussen DL, Kenneson A, Conn PJ, et al. (1999) Fragile X mouse: strain effects of knockout phenotype and evidence suggesting deficient amygdala function. Neuroscience 94 1: 185–192. doi: 10.1016/s0306-4522(99)00285-7
- 38. Lauterborn JC, Rex CS, Kramár E, Chen LY, Pandyarajan V, et al. (2007) Brain-derived neurotrophic factor rescues synaptic plasticity in a mouse model of fragile X syndrome. J Neurosci. 27 40: 10685–10694. doi: 10.1523/jneurosci.2624-07.2007
- 39. Moy SS, Nadler JJ, Young NB, Nonneman RJ, Grossman AW, et al. (2009) Social approach in genetically engineered mouse lines relevant to autism. Genes Brain Behav. Mar 8(2): 129–42. doi: 10.1111/j.1601-183x.2008.00452.x
- 40. Spencer CM, Alekseyenko O, Hamilton SM, Thomas AM, Serysheva E, et al. (2011) Modifying behavioral phenotypes in Fmr1KO mice: genetic background differences reveal autistic-like responses. Autism Res. Feb 4(1): 40–56. doi: 10.1002/aur.168
- 41. Shu W, Cho JY, Jiang Y, Zhang M, Weisz D, et al. (2005) Altered ultrasonic vocalization in mice with a disruption in the Foxp2 gene. Proc Natl Acad Sci U S A. 102 27: 9643–9648. doi: 10.1073/pnas.0503739102
- 42. Heyser CJ (2004) Assessment of developmental milestones in rodents. Curr Protoc Neurosci. Chapter 8:Unit 8.18.
- 43. Rogers DC, Fisher EM, Brown SD, Peters J, Hunter AJ, et al. (1997) Behavioral and functional analysis of mouse phenotype: SHIRPA, a proposed protocol for comprehensive phenotype assessment. Mamm Genome. 8(10): 711–3. doi: 10.1007/s003359900551
- 44. Nadler JJ, Moy SS, Dold G, Trang D, Simmons N, et al. (2004) Automated apparatus for quantitation of social approach behaviors in mice. Genes Brain Behav. 3(5): 303–14. doi: 10.1111/j.1601-183x.2004.00071.x
- 45. Elder GA, Ragnauth A, Dorr N, Franciosi S, Schmeidler J, et al. (2008) Increased locomotor activity in mice lacking the low-density lipoprotein receptor. Behav Brain Res. 191(2): 256–65. doi: 10.1016/j.bbr.2008.03.036
- 46. Gross C, Nakamoto M, Yao X, Chan CB, Yim SY, Ye K, Warren ST, Bassell GJ (2010) Excess phosphoinositide 3-kinase subunit synthesis and activity as a novel therapeutic target in fragile X syndrome. J Neurosci. 2010 Aug 11 30(32): 10624–38. doi: 10.1523/jneurosci.0402-10.2010
- 47. Sakurai T, Dorr NP, Takahashi N, McInnes LA, Elder GA, et al. (2011) Haploinsufficiency of Gtf2i, a gene deleted in Williams Syndrome, leads to increases in social interactions. Autism Res. 2011 Feb 4(1): 28–39. doi: 10.1002/aur.169
- 48. Bozdagi O, Wang XB, Nikitczuk JS, Anderson TR, Bloss EB, et al. (2010) Persistence of coordinated long-term potentiation and dendritic spine enlargement at mature hippocampal CA1 synapses requires N-cadherin. J Neurosci. 28; 30 (30): 9984–9. doi: 10.1523/jneurosci.1223-10.2010