Double strand breaks (DSBs) can be repaired via either Non-Homologous End Joining (NHEJ) or Homology directed Repair (HR). Telomeres, which resemble DSBs, are refractory to repair events in order to prevent chromosome end fusions and genomic instability. In some rare instances telomeres engage in Break-Induced Replication (BIR), a type of HR, in order to maintain telomere length in the absence of the enzyme telomerase. Here we have investigated how the yeast helicase, Mph1, affects DNA repair at both DSBs and telomeres. We have found that overexpressed Mph1 strongly inhibits BIR at internal DSBs however allows it to proceed at telomeres. Furthermore, while overexpressed Mph1 potently inhibits NHEJ at telomeres it has no effect on NHEJ at DSBs within the chromosome. At telomeres Mph1 is able to promote telomere uncapping and the accumulation of ssDNA, which results in premature senescence in the absence of telomerase. We propose that Mph1 is able to direct repair towards HR (thereby inhibiting NHEJ) at telomeres by remodeling them into a nuclease-sensitive structure, which promotes the accumulation of a recombinogenic ssDNA intermediate. We thus put forward that Mph1 is a double-edge sword at the telomere, it prevents NHEJ, but promotes senescence in cells with dysfunctional telomeres by increasing the levels of ssDNA.
Citation: Luke-Glaser S, Luke B (2012) The Mph1 Helicase Can Promote Telomere Uncapping and Premature Senescence in Budding Yeast. PLoS ONE 7(7): e42028. https://doi.org/10.1371/journal.pone.0042028
Editor: Arthur J. Lustig, Tulane University Health Sciences Center, United States of America
Received: April 19, 2012; Accepted: June 29, 2012; Published: July 27, 2012
Copyright: © 2012 Luke-Glaser, Luke. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: S.L.-G. is supported by a long term EMBO fellowship. This work was supported by the Netzwerk Alterns-Forschung, which is funded from the Ministerium für Wissenschaft, Forschung und Kunst Baden-Württemberg. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
In the absence of telomerase, telomeres progressively shorten with each cell division and eventually, when they are critically short, get recognized as DNA damage due to the inability to maintain their protective cap structure , . Critically short telomeres activate a checkpoint response leading to cell cycle arrest and eventual cellular senescence. In rare instances, cells acquire the ability to maintain their telomeres via a homology-directed repair (HR) mechanism, and thereby evade checkpoint-mediated arrest . In yeast, such cells are referred to as survivors, whereas human cells using HR to maintain telomeres have been named ALT (Alternative Lengthening of Telomeres) cells . Interestingly, although most human cancers up-regulate telomerase, about 15% of human cancers maintain their telomeres through the ALT pathway . BIR has been proposed to be the underlying mechanism in survivor establishment, as yeast mutants unable to perform BIR, i.e. lacking the non-essential DNA polymerase ∂ subunit Pol32, are also defective in forming survivors . BIR is specifically initiated at a one-ended break that can arise at a critically short telomere or from a replication fork collapse , . The invasion of one end results in the formation of a D (dissociation) loop, whereby a uni-directional replication fork is established and subsequently gets elongated. Indeed, BIR is suppressed at a DSB, where both ends share homology with a template, in order to prevent loss of heterozygosity (LOH) and to allow a more classical gene conversion (GC) reaction to carry out repair of the DSB . Both the yeast homolog of the Bloom helicase, Sgs1, and the exonuclease, Exo1, are able to inhibit the BIR reaction at a double-stranded break in yeast when over-expressed .
The budding yeast (S. cerevisiae) helicase Mph1 (mutator phenotype 1) is a putative homolog of the Fanconi Anemia protein M (FANCM) . Fanconi Anemia is a heritable disease associated with bone marrow failure, genomic instability and early onset cancer . Fanconi anemia genes are overexpressed in melanoma cells  and were implicated in resistance to chemotherapeutics in various tumors , . In yeast, deletion, as well as overexpression of MPH1 leads to genomic instability , . Although extensive genetic studies have been carried out with mph1Δ mutants , , the in vivo function of Mph1 remains unclear. Interestingly, in vitro, Mph1 has the ability to displace D-loops  suggesting that it may play a role in either preventing inappropriate recombination events or perhaps in their resolution. Here we have used an overexpression approach to gain insight into Mph1’s in vivo function.
Yeast Strains, Plasmids and Culture Media
All yeast strains were grown following standard protocols and using common culture media. The following strains used in these studies are derivatives of BY4741 (his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) unless stated otherwise. Double mutants were constructed through standard mating and tetrad dissection procedures, except for the BIR strain, where MPH1 was deleted by integrating a previously amplified gene cassette. The BIR strain JRL 347 was a gift from the Haber lab. Strains used to assay GC and NHEJ were kind gifts from the Gasser Lab : GC: GA-1080 parental strain from the GAL HO strains, GA-2321 ade3::GALHO MATinc-URA3 and RAD52 disrupted in GA2321. NHEJ strains: GA-1080 parental strain from the GAL HO strains, JKM179, ku70::URA3 JKM181. WT MPH1 and mph1E210Q were cloned via PstI and SalI into pRS425 (pGal, Leu2 and pGPD, Leu2). A HA-tag was included to check expression by Western Blotting. In order that the C-terminal HA tag does not interfere with Mph1 activity, a flexible RGS (arginine, glycine, serine) linker was introduced immediately after the open reading frame of MPH1. The forward primer included the sequence: 5-CGCCTGCAGATGGCTAGTGCAGATGATTAC-3, reverse primer: 5-GCCGTCGACTCACAAAGAAGCGTAATCTGGAACATCATATGGGTAGGCAGAACCTCTAAAATCAGAATCTGAGCCCAG-3. EXO1 was cloned similarly with a C-terminal HA tag separated from the EXO1 ORF via an RGS linker into pGPD, Leu2, 2micron. The following primers containing and HindIII or a XhoI were employed: 5′-CGCAAGCTTATGGGTATCCAAGGTCTTCTTCC-3′; 5′- GCCCTCGAGTCACAAAGAAGCGTAATCTGGAACATCATATGGGTAGGCAGAACCTCTTTTACCTTTATAAACAAATTGGG-3′. The plasmids used in the rad52Δ est1Δ senescence assay were gifts from the Johnson lab (pAG415GAL-ccdB, PAG415GAL-MPH1, centromeric, Leu2).
Senescence and Survivor Assay in Culture
Heterozygous diploids est1Δ/EST1 were transformed with plasmids and sporulated in liquid sporulation medium containing 1% potassium acetate and 0.005% zinc acetate. For the liquid growth assay, isogenic single and double mutants were diluted to an OD600 of 0.01 and grown in YPD for 24 hours at 30°C. The OD600 was determined and the culture was re-diluted to an OD600 of 0.01. This procedure was repeated for up to 10 days or until survivors had formed. The number of population doublings was calculated with the following formula: Log2 (OD600/0.01). For the senescence assays in galactose-containing medium population doublings were counted from when plasmid expression by galactose was initiated. For the senescence assay in YPD medium the 25 population doublings on the tetrad dissection plate were taken into account.
Cells were grown over night in 1% raffinose medium lacking leucine to maintain the MPH1 or the empty vector. Saturated cultures were diluted to an OD600 = 0.5 and serial tenfold dilutions were spotted on SD-Leu and SGal-Leu plates. After 3 days at 30°C the plates were replica plated onto canavanine and hygromycin plates to confirm that repair occurred via BIR. To quantify the efficiency of BIR, approximately 200 cells of the overnight culture were plated onto SD-Leu and SGal-Leu plates respectively and colonies were counted. Five independent cultures for the vector control and four GAL-MPH1 transformed colonies were analyzed for their capacity to repair BIR, which was calculated as growth on galactose/growth on glucose.
HO and NHEJ Assay
Cells were grown over night at room temperature in media lacking leucine and containing 1% raffinose. Cells were diluted to an OD600 = 0.5 and tenfold dilutions were spotted on glucose and galactose plates lacking leucine. Cells were grown 2-3 days at 30°C. Deletion of RAD52 served as a control for a strain defective for repair by homologous recombination (HR), whereas disruption of KU70 leads to a deficiency in non homologous end joining (NHEJ).
Approximately 10 OD600 units of cells were pelleted and DNA was extracted using the puregene yeast kit (Qiagen). 100 ng DNA was denatured for 10 minutes at 96°C and a cytosine (C)-tail was added by terminal transferase (NEB) while incubating for 30 minutes at 37°C. After heat inactivation of the terminal transferase for 10 minutes at 65°C, telomeric DNA was amplified by Phusion polymerase (Finnzymes). The reverse primer annealing with the C-tail was: CGGGATCCG18. The following forward primers were used in the PCR reactions: 5-TTAGGGCTATGTAGAAGTGCTG-3 (Y’ of 12L), 5-GCGGTACCAGGGTTAGATTAGGGCTG-3 (1L) and 5-AAATGAGGACTGGGTCATGG-3 (6R). The primers (all 1 µM final) were allowed to anneal for 15 seconds at 63°C followed by 20 seconds of elongation at 68°C (45 cycles). The PCR products were separated on a 1.8% agarose gel. The fragment size was analyzed with the help of Multi Gauge software (Fuji). The primer lengths were substracted and the length distribution was presented graphically.
ssDNA dot Blot and Southern Blot
For the ssDNA dot blot cultures of cells were grown in raffinose containing medium at 23°C to log phase, 2% galactose was added to induce MPH1 expression and cells were arrested for three hours with in 20 µg/ml nocodazole (Applichem). Cultures were then shifted to 27°C by adding the appropriate amounts of pre-warmed medium containing nocadozole. DNA was extracted under native conditions (Puregene kit, Qiagen). The 65°C step was omitted to prevent denaturation of DNA. 3 µg of DNA was spotted onto a nylon membrane (Amersham). For the loading control 0.25 µg of DNA was denatured with 0.2 M NaOH for 15′ at 65°C. Membrane was pre-hybridized for 30′ at 47.5°C. The telomeric C- (CACCACACCCACACACCACACCCACA) and G-(GTGGGTGTGGTGTGTGGGTGTGGTG) probes were DIG-labeled (Roche High grade kit) and allowed to hybridize over night at 47.5°C. The membrane was washed twice in 2×SSC 0.1% SDS and twice in 0.5×SSC 0.1%SDS at 47.5°C. The membrane was blocked and hybridized with AP-coupled antibody against DIG (Roche) for 30 minutes at room temperature. The signal was detected by CDP-star solution (Roche) and quantified by the ImageJ software. ssDNA was digested for 2 hours at 37°C by 20 units of Exonuclease I (E. coli, NEB) in a total volume of 15 ml.
For Southern blotting the procedure was as described above, but the DNA (25 µg) was denatured for 1 hour at 65°C and digested for 4 h with XhoI prior to loading and separting on a 1.2% agarose gel. The DNA was transferred onto a nylon membrane at 1A for 2 hours at 4°C. Antibody binding, washing and detection were identical to the ssDNA blot.
Telomere Fusion Assay
Was carried out using Lev1212 and Lev728 (rap1-(Δ)) as previously described . Sequences for HIS4 PCR are:
5-GACGCTCCAGAGGAATCTTA-3 and 5-TTGGTCTGCTCAAAGCCTTC-3. The PCR reaction was carried out by Phusion HF (Finnzymes).
Mph1 Abolishes Break-Induced Replication at a DSB, but Allows Survivor Formation
Based on its in vitro activity of D-loop displacement , we tested the notion that Mph1 may inhibit homology directed repair (HR) in vivo. We used a previously described reporter system . Briefly, the reporter strain harbors an HO endonuclease recognition site on chromosome V that can be cleaved by a galactose-inducible HO endonuclease. The resulting DSB is flanked only on one side by a sequence (CA) that bears homology (A) to a template on chromosome XI (AN1). Due to this ‘one-sided’ homology the DSB can only be repaired through BIR and cells can only grow on galactose-containing plates after successful repair by BIR. The restoration of the CAN1 gene leads to canavanine sensitivity, whereas loss of the HPH gene confers hygromycin sensitivity (Figure 1A). We show that MPH1 overexpression specifically abolishes growth on galactose-containing plates and therefore conclude that Mph1 inhibits the repair of a double-strand break (DSB) via BIR (Figure 1A, 1B). Consistently, the repair frequency by BIR was slightly increased in cells carrying a MPH1 deletion (Figure 1B, right panel). DSBs that are flanked on both sides by sequences that share homology to a template elsewhere in the genome get efficiently repaired by gene conversion (GC). If no homologous sequences are available, the DSB is repaired by NHEJ (non-homologous end joining). Strikingly, repair via GC (Figure 1C) and NHEJ  (Figure 1D) were not affected by Mph1 overexpression. As expected, GC and NHEJ were abolished upon deletion of RAD52 and YKU70, respectively. Mph1 is a member of the DEAH family of ATP-dependent helicases . The DEAH motif is required for the complete inhibition of BIR, as a DEAH-mutant that lacks ATPase activity in vitro  is a less potent inhibitor of BIR (Figure 1B).
(A) Cells harboring the indicated constructs on chromosomes V and XI were grown overnight in raffinose and spotted as 10-fold serial dilutions on either glucose or galactose containing media (to induce an HO DSB). Growth on galactose and subsequent canavanine and hygromycin sensitivity indicates that those cells were able to repair an HO induced DSB via BIR directed repair. This is due to the fact that following BIR the hygromycin resistance cassette (HPH) was lost and the canavanine sensitivity marker (CAN1) was re-constituted due to homology between the “A’s”, (for more detailed description see ). (B) Cells harboring the constructs described in (A) were grown over night in raffinose and spread onto glucose and galactose plates, colonies were counted and the survival ratio of galactose/glucose indicated the percentage of cells that were able to repair a DSB by BIR. Upon overexpression of wild-type MPH1 cells were unable to repair the DSB via BIR. Mutations in the DEAH domain (D209RE210R) partially restored the ability to perform repair via BIR (left panel). The mph1Δ (n = 8) and corresponding WT (n = 4) strains were grown in YPD medium overnight and spread on YPD and YP medium containing 2% galactose. mph1Δ cells show an increased rate of repair by BIR right panel. (C) An HO cut in the MAT locus results in gene conversion due to the fact that there is homology on both sides of the HO site in the MATinc locus on chromosome V (upper panel). Cells were grown and spotted as in (A), and rad52Δ cells were used as a positive control for cells that were defective for GC. Unlike BIR, over-expression of MPH1 did not affect the efficiency of gene conversion, as seen through robust growth on galactose plates (lower panel). (D) When no homologous MAT locus is available the HO cut must be repaired through NHEJ (upper panel). As expected, yku70Δ cells are defective for NHEJ and fail to grow on galactose plates, whereas overexpression of MPH1 has no effect on NHEJ when compared to wild type cells harboring an empty vector (lower panel).
As BIR has been implicated in the formation of survivors in the absence of telomerase activity, we monitored est1Δ mutant yeast cultures during senescence and recovery in the presence of MPH1 overexpression. Est1 (ever shorter telomere 1) recruits the catalytic telomerase subunit Est2 to telomeres and is essential for telomerase mediated telomere elongation . The optical density of the indicated yeast cultures (OD600) was plotted on the y-axis as an estimate of cell growth with respect to population doublings (PD) following a daily dilution/re-growth protocol (see materials and methods). Importantly, we confirmed that the OD600 measurement was an accurate representation of cell number through careful cell counting experiments (unpublished results). As expected est1Δ cells harboring an empty vector lose viability with increasing PDs due to telomere loss, however HR dependent survivors eventually take over the culture and restore nearly wild type growth rates (Figure 2A, est1Δ v). Surprisingly, MPH1 overexpression did not inhibit survivor formation in an est1Δ mutant background, however it did accelerated the rate of senescence (Figure 2A). This effect was specific to cells lacking EST1, as there was no obvious loss of viability in wild type cells overexpressing Mph1 (Figure 1A). The continued expression of MPH1 in survivors was confirmed by Western Blotting (Figure S1A). The typical appearance of multiple heterogenous telomeric bands on a Southern blot confirmed that type II survivors are being formed (Figure S1B), i.e. the telomeric tract has been amplified rather than the subtelomere as is the case for type I survivors. In contrast to overexpression of MPH1, deletion of MPH1 slightly delayed the senescence in est1Δ cells (Figure 2B). We next asked whether another factor that inhibits BIR at a DSB, Exo1 , showed a similar tendency. Similar to MPH1 overexpression, we observed faster senescence, without inhibition of survivor formation (Figure 2C) in an est1Δ culture when EXO1 was overexpressed. Both MPH1 and EXO1 overexpression accelerated senescence without inhibition of survivor formation in est2Δ cells as well, ruling out that this effect was specific to MPH1 overexpression in est1Δ associated senescence (Figure 2D). By analyzing the curves of each individual clone (Figure S1C) we realized, that although survivors were able to form, those that overexpressed MPH1 or EXO1 experienced greater instabilities in growth rate.
(A) An est1Δ/EST1 heterozygous diploid was transformed with either empty vector or MPH1 fused to a galactose inducible promoter on a 2µ plasmid. Cells were sporulated, microdisected and MPH1 expression was turned on with 2% galactose. Liquid senescence assays were performed on the indicated genotypes at 30°C and diluted daily to an OD600 0.01 (see materials and methods for detailed description). Mean and SEM are displayed. (est1Δ v: n = 5, est1Δ MPH1: n = 6, WT v: n = 4, WT MPH1: n = 4). est1Δ pGAL::MPH1 cells lose viability faster than est1Δ v cells. The increased rate of senescence is not due to the toxicity of MPH1 overexpression, as shown in wildtype (WT cells). (B) Deletion of MPH1 delays senescence in est1Δ cells (est1Δ : n = 3, est1Δ mph1Δ: n = 7) cultured in YPD medium. (C) Overexpression of EXO1 also leads to accelerated senescence in est1Δ cells (est1Δ v: n = 4, est1Δ EXO1: n = 6). (D) The MPH1 and EXO1 overexpression effects on senescence are also present in est2Δ cells (n = 4 for pGAL::EXO1 and pGAL::MPH1, n = 3 for pGAL) (E) High-levels of Mph1 promote senescence even in the absence of homologous recombination (rad52Δ) and telomerase activity (est1Δ v: n = 2, est1Δ rad52Δ v: n = 4, est1Δ MPH1: n = 3, est1Δ rad52Δ MPH1: n = 3). For the latter assay MPH1 was expressed from a galactose-inducible promoter on a centromeric plasmid.
Rad52 is required for most types of HR. The deletion of RAD52 has been reported to compromise growth in an est1Δ background and leads to rapid senescence . Surprisingly, MPH1 overexpression was additive with the deletion of RAD52 in the est1Δ background in terms of accelerating senescence (Figure 2E). This indicates that Mph1 promotes senescence through a mechanism that is independent of HR inhibition. Accelerated telomere shortening could potentially account for the faster senescence in MPH1 overexpressing cells. We measured telomere length and found that bulk telomere length was not significantly changed in either est1Δ or wild type cells overexpressing MPH1 compared to vector control cells, as determined by telomeric PCR amplifying telomere 1L (Figure S1D) and Y’ (Figure 3B and C). Similarly, the faster senescence in est1Δ cells overexpressing EXO1 cannot be explained by loss of telomeric sequences, as there was no difference in length compared with control cells (Figure S1E). Nevertheless, we cannot exclude that MPH1 overexpression leads to single short telomeres that could lead to premature senescence . Together, the data presented in Figures 1 and 2 supports the notion that high cellular levels of Mph1 can prevent BIR at a DSB, but not at a telomere that is being maintained by HR. Moreover, overexpression of MPH1 leads to a rapid senescence phenotype. However the inhibition of RAD52 mediated HR does not account for the rapid senescence as MPH1 overexpression increased the senescence rate even further in a rad52Δ genetic background. This indicates that another unidentified Mph1-mediated alteration at the telomere may be affecting senescence.
(A) The fast loss of viability phenotype in est1Δ depends on the DEAH domain, as the double arginine ‘mph1 RR’ mutant described in Figure 1B leads to a partial rescue of the early onset senescence. (B) Genomic DNA of the senescence assay described in (A) was extracted after about 9 and 17 Population doublings. The Y’ telomeres were amplified by PCR and run on an agarose gel. (C) Quantification of the PCR products does not reveal any difference in Y’ telomere length of est1Δ cells expressing either the empty vector, MPH1 or the helicase mutant. (D) Genomic DNA was extracted under native conditions after about 9 population doublings. DNA of three independent cultures for vector control, MPH1 and mph1 RR expressing cells was spotted on a nylon membrane. Telomeric ssDNA was revealed by annealing with a DIG-labeled telomeric C-rich oligonucleotide. DNA samples were denatured and submitted to dot blotting to control for equal loading. (E) The signal for ssDNA from (D) was quantified and normalized by the total amount of telomeric DNA. Overexpression of MPH1 leads to an increased amount of telomeric ssDNA. This increase is partially suppressed upon overexpression of the mph1 RR mutant. (F) The ssDNA extracted under native conditions (Figure 3D) was digested for 2 hours with bacterial Exo1, which digests 3′-overhang DNA in the 3′ to 5′ direction. Samples were blotted on a nylon membrane and revealed by a DIG-labeled oligo-C-probe. Denatured DNA was used as a loading control. (G) Quantification of blotted ssDNA from (C).
Mph1 Overexpression Leads to the Accumulation of ssDNA at the Telomere
Resection of a double-stranded break leads to the accumulation of ssDNA that subsequently promotes HR. It has been previously reported that increased rates of senescence can be attributed to the accumulation of ssDNA at the telomere and the subsequent activation of a Mec1-dependent checkpoint . We tested whether the accumulation of ssDNA at telomeres upon MPH1 overexpression may be responsible for the rapid senescence and furthermore whether this depended on Mph1’s DEAH domain (Figure S2A).
Like the inhibition of BIR at a DSB, early onset senescence requires an intact DEAH domain, as overexpression of the mph1 D209RE210R mutant (Figure S2B) only partially accelerates senescence (Figure 3A). We extracted genomic DNA after 9 and 17 population doublings in galactose-containing medium and confirmed that in our est1Δ cultures bulk telomere length did not change due to overexpression of MPH1 or mph1 D209RE210R (Figure 3B and C).
As aforementioned, we hypothesized that the high level of Mph1 could potentially lead to the accumulation of ssDNA at the telomere. Therefore we extracted genomic DNA from pre-senescent est1Δ cells from Figure 3A, and subjected it to a dot blot analysis under both non-denaturing and denaturing conditions (Figure 3D). Overexpression of MPH1 led to a strong increase in the signal for telomeric G-rich DNA under native conditions in our est1Δ strain (Figure 3D and E). Denatured DNA served as a loading control. The ssDNA was quantified and normalized to the total amount of telomeric DNA spotted on the membrane (Figures 3D and E). Overexpression of the mph1 D209RE210R mutant showed an intermediate phenotype, indicting that the helicase domain is partially responsible for the accumulation of ssDNA at the telomere. In order to determine whether the ssDNA was due to an increased 3′ terminal overhang or the accumulation of internal DNA replication intermediates, we digested the native DNA with bacterial Exo1, which only removes terminal ssDNA in a 3′ to 5′ direction. The increase in ssDNA upon MPH1 over-expression was strongly reduced upon digestion with Exo1 (Figures 3F and G) indicating that 3′ overhang accumulates upon MPH1 over-expression. To further rule out that the ssDNA at the telomere stems from replication intermediates, we probed the native DNA with an oligo-G probe that hybridizes to the C-strand. We could not detect, any accumulation of ssDNA on the C-strand (Figure S2C and S2D). Together these data reveal that Mph1 leads to a telomeric alteration that becomes more accessible to nucleases in (uncapped) pre-senescent est1Δ cells.
Overexpression of MPH1 is Toxic in Mutants that are Defective in Telomere Capping
Telomeres become uncapped with shortening of the telomeric tract. Telomere capping can also be promoted through two independent protein complexes: the CST (Cdc13, Stn1, Ten1) complex and the Ku70/Ku80 heterodimer. Upon inhibition of either the CST complex or the Ku70/Ku80 complex, the telomeric 5′ strand (C-rich) gets rapidly degraded in a manner that largely depends on Exo1. Since we have observed that overexpression of MPH1 leads to uncapping in pre-senescent cells we predicted that mutants in the capping pathways may be particularly sensitive to MPH1 overexpression. Indeed, overexpression of MPH1 is toxic in cdc13-1, stn1-13 and ku70Δ mutants at semi-permissive temperature (Figure 4A). In order to better understand the nature of MPH1 toxicity in cdc13-1 cells, we overexpressed MPH1 in wild type cells and cdc13-1 mutants from a galactose-inducible promoter in nocodazole for 3 hours at permissive temperature, to prevent differences in the samples arising from altered progression through the cell cycle. Cells were then shifted to the semi-permissive temperature for the cdc13-1 allele (27°C) and samples were taken before the shift and both 90 and 120 minutes following the temperature shift. DNA was extracted under non-denaturing conditions and spotted onto a nylon membrane. The amount of ssDNA (G-strand) was detected by a C-rich telomeric probe and normalized by the total amount of telomeric DNA spotted (after denaturation). As expected  cdc13-1 mutants accumulate ssDNA at non-permissive temperature (Figures 4B and 4C). Strikingly, we observed that overexpression of MPH1 exacerbates the accumulation of ssDNA in the cdc13-1 mutant background, whereas overexpression in wild type cells did not have an effect (Figure 4B and 4C). We tested several mph1 point mutations for toxicity in cdc13-1 (Figure S3). Surprisingly, mutants that are catalytically inactive in vitro like E210Q or D209NE210Q still decreased the viability of a cdc13-1 and a cdc13-1 mph1Δ strain (Figure S3B and S3C). Inversion of the electric charge in mph1 D209RE210R led to a decrease in toxicity. Wild type as well as mph1 mutant proteins were expressed at similar levels, as assessed by Western Blotting (Figure S3D).
(A) Galactose induced over-expression of MPH1 causes lethality in cdc13-1, stn1-13 and ku70Δ mutants at the respective semi-permissive temperature. (B) WT and cdc13-1 cells either expressing the empty vector or MPH1 fused to the galactose-inducible promoter were arrested with nocodazole for three hours and shifted to the semi-permissive temperature for the cdc13-1 allele (27°C). Samples were taken prior to shift (‘pre’), 90 and 120 minutes after the temperature shift. DNA was extracted under non-denaturing conditions and spotted onto a nylon membrane. Telomeric ssDNA was revealed by annealing with a DIG-labeled oligonucleotide probe containing telomeric repeats. DNA was denatured and spotted on a nylon membrane and revealed as aforementioned for controlling the loading of total amounts of DNA. (C) The chemiluminescence signal of the blot in (B) was quantified and the ssDNA was normalized by the amount of total DNA.
Mph1 Inhibits NHEJ-dependent Fusions at the Telomere
The C-terminus of Mph1 has been shown to interact with RPA . RPA’s primary function is to stabilize ssDNA. As overexpression of MPH1 leads to increased levels of ssDNA at telomeres and hence faster senescence, we wondered whether the interaction between Mph1 and RPA was crucial to promote senescence. To test this hypothesis, we overexpressed MPH1 and an mph1 mutant lacking the C-terminal residues crucial for interaction with RPA  (mph1ΔCter, Figure S4A and S4B). Indeed overexpression of mph1ΔCter in an est1Δ background did not accelerate senescence in comparison with high levels of full-length MPH1 (Figure 5A). Consistently, the overxpression of the mph1ΔCter is not as toxic in cdc13-1 cells as compared to overexpression of the full length Mph1 protein (Figure 5B and Figure S4C for Western Blot). Taken together, the interaction between Mph1 and the ssDNA binding protein RPA is crucial to inhibit the growth of cells with impaired telomere integrity.
(A) Mph1 interacts with the ssDNA binding protein RPA via the C-terminal of Mph1. Deletion of the C-terminal part of Mph1 completely abolishes the acceleration of senescence in est1Δ cultures. (B) Overexpression of mph1ΔCter is less toxic than overexpression of the wildtype MPH1 in a cdc13-1 temperature-sensitive strain. Strains were grown at semi-permissive temperature under inducing (galactose) and non-inducing conditions. (C) Whether a cell undergoes repair by HR or NHEJ is decided at the level of resection, as a ssDNA overhang is required for HR, but not for NHEJ. We propose that Mph1 leads to increased telomeric resection at a dysfunctional telomere and thereby promotes recombination, i.e. faster rate of survivor formation. This leads to the prediction that MPH1 overexpression should limit chromosome end-to-end fusion, which arise via NHEJ. (D) Strains of the indicated phenotypes were allowed to reach stationary phase by growing them for 5 days in liquid culture. A strain containing the Rap1 degron allele (rap1-(Δ served as a positive control for high levels of chromosome end to end fusions. In this strain Rap1 function is impaired in stationary phase at 30°C . Overexpression of MPH1 and EXO1 from a constitutive GPD-promoter containing plasmid decreased the amount of chromosome end-to-end fusions fusions between Y’ telomeres in a decapping rap1-(Δ mutant. Deletion of MPH1 had no effect on the occurrence of telomeric fusions. Fusions were detected via telomere PCR and agarose gel electrophoresis. PCR of the HIS4 gene served as loading control.
We hypothesized that increased resection at the telomere could potentially promote HR and concomitantly inhibit NHEJ (Figure 5C) since NHEJ requires a blunt end for ligation. NHEJ can lead to deleterious fusions between telomeres and has to be suppressed at chromosome ends in order to conserve genome integrity. We used a previously described PCR-based approach to test for NHEJ-dependent fusions between . Only if two telomeres are fused, can they be amplified by PCR using two forward primers (P1 and P2) specific for different chromosome ends (Figure 5D upper panel). A sensitized rap1-(Δ) background was used, as this strains shows a high incidence of inter-telomeric fusions  (Figure 5D, lower panel). Indeed, overexpression of MPH1 drastically reduced the amount of chromosome end-to-end fusions in the rap1-(Δ) strain (Figure 5D and Figure S4D). Overexpression of the exonuclease, EXO1, phenocopied our observations with MPH1 overexpression whereas the deletion of MPH1 did not lead to more fusions (Figure 5D, right panel). PCR of the HIS4 gene was used to verify equal input of genomic DNA for the PCR reaction. Thus high levels of Mph1 and Exo1 inhibit telomere fusions, but on the other hand lead to increased levels of ssDNA and growth arrest in cells bearing dysfunctional telomeres.
Mph1 seems to play the role of a double-edge sword at the telomere: while overexpressed MPH1 can inhibit deleterious fusions between chromosome ends, it can also cause growth inhibition and rapid senescence in cells defective for telomere capping. The underlying mechanism seems to be the regulation of ssDNA levels. High ssDNA levels promote HR due to the accumulation of invasive Rad51-coated molecules whereas NHEJ-dependent fusions are inhibited by ssDNA due to the requirement of a blunt end. We hypothesize that the levels of Mph1 have to be kept in check at the telomere in order to establish the correct balance between senescence, HR and NHEJ inhibition, ensuring that the above mentioned processes are either inhibited or promoted at the right time according to the functional state of the telomere. It has been previously shown that the levels of the human homolog of Mph1, FANCM, are controlled via ubiquitin-dependent degradation after DNA damage  and in mitosis . Therefore, it will be important to investigate, whether Mph1 levels are also regulated via ubiquitination at the telomere.
Interestingly, FANC proteins have recently been shown to inhibit NHEJ at DSBs and drive repair towards HR, much like what we have seen with Mph1 at the telomere. Indeed FANCD2 was proposed to compete with the NHEJ-factor Ku70 for access to DSBs in both chicken and human cells . Furthermore, the FANCD2 homolog in C.elegans has been reported to prevent erroneous repair of DSBs by NHEJ during meiosis, again by promoting HR . Thus, FANC proteins seem to be instrumental in regulating the choice of repair pathway at a DSB. In this respect it may not be surprising that the FANC proteins also have an important role at chromosome ends, as telomeres structurally resemble DSBs in many respects . Whereas, the inhibition of NHEJ at telomeres is essential in order to prevent chromosome fusions, a source of genomic instability, NHEJ is often the choice of repair at a DSB. Our results suggest that Mph1-mediated repair decisions at telomeres are differently regulated from repair at a DSB. While MPH1 overexpression potently inhibits NHEJ at the telomere (Figure 5D), it does not affect NHEJ-mediated repair at an internal DSB (Figure 1D). Along the same lines, MPH1 can completely abolish BIR at a DSB, however allows recombination (survivor formation) to occur at the telomere. Consistent with the notion that MPH1 does inhibit HR at telomeres, we found that MPH1 overexpression in rad52Δest1Δ cells exacerbated the early onset of senescence (Figure 2E). The abnormally high levels of ssDNA in est1Δ mutant cultures overexpressing MPH1 (Figure 3D and 3E) are likely accountable for the early senescent phenotype. With MPH1 overexpression we found no evidence of excessive bulk telomere shortening, although we cannot rule out that single short telomeres may arise more frequently.
How could the action of Mph1 lead to increased levels of telomeric ssDNA? In mammalian cells telomeres have been shown to form a lasso-like structure (T-loop) , where 3′overhang invades the telomeric dsDNA, resulting in the formation of a Dissociation (D)-loop. Yeast telomeres have also been shown to form a fold-back structure , , ,  although the characteristics of these loop structures are less well described than those of mammalian T-loops. Mph1 has been shown to undo D-loops in vitro , via its helicase activity. Although the function of T-loops remains enigmatic, they have been proposed to play a protective role, by sequestering the telomeric overhang and thereby preventing checkpoint activation and unscheduled recombination. Interestingly, Mph1 has the same in vitro activities as RTEL1 (Regulator of telomere elongation) and RTEL1 has been recently shown to open T-loops in vitro in an ATP-ase dependent manner . FANCM is another candidate for a T-loop regulator as it can also disassemble D-loops in vitro . It will be interesting to determine whether Mph1, FANCM and RTEL1 have the ability to affect T-loops structure in vivo. The disassembly of T-loops could explain how exonucleases gain increased access to telomeres and thereby increase the levels of ssDNA when MPH1 is overexpressed. We propose that Mph1 may have two mechanisms of function in vivo one depending on its helicase activity, probably by undoing a D-loop intermediate and the other one being a scaffolding function independent of its ATPase/helicase activity possibly by recruiting and or stimulating nucleases. Interestingly, it has been published that Mph1 stimulates the endonuclease activities of both Fen1 and Dna2 in vitro  and that this stimulation in independent of the ATPase/helicase activity of Mph1. This would be in agreement with our observation that over-expression of helicase-dead Mph1 still leads to slightly elevated levels of ssDNA at the telomere in est1Δ cells (Figure 3D and E). Another, less likely, possibility would be that the helicase dead mutants are still active to an extent in vivo, despite their full loss of activity in vitro. It is noteworthy that the proposed human homolog of Mph1, FANCM, also plays a dual role: the role in checkpoint activation and replication fork progression depends on its ATPase activity , , whereas the recruitment of the Fanconi anemia core complex to chromatin (its scaffolding function) occurs independent of its ATPase domain .
The RTEL1 locus is amplified in many human tumors and upregulation of RTEL1 led to liver malignancies in mice . Similarly, FANC proteins including FANCM were found to be transcriptionally upregulated in melanomas . Furthermore, the FANC pathway was implicated in resistance to chemotherapeutic agents , . Therefore, we think it is crucial to not only understand the mechanism of action of these helicases, but also how to keep them in check.
(A) Western Blot showing the expression of MPH1-HA in two independent cultures of est1Δ survivors. Actin served as loading control. (B) DNA of survivors was extracted, digested by XhoI and analyzed by Southern blotting using a G-rich telomeric probe. The appearance of the smeared multiple bands in the vector-control (n = 3) and the MPH1-overexpressing cells (n = 3) shows that an amplification of telomeric repeats has occurred. This is typical for typeII survivors. (C) Individual curves of each culture expressing either empty vector of MPH1 or EXO1 from a galactose-inducible promoter. (D) Quantification of telomere length PCRs for telomere 1L corresponding to the senescence assay presented in Figure 2A (n = 3 for est1Δ v and MPH1, n = 2 for WT v and MPH1). (E) Quantification of Y’ telomere length by PCR for the senescence assay in est1Δ cells overexpressing EXO1 (n = 3 each). Overexpression of EXO1 does not change telomere length in an est1Δ background. (F) Quantification of Y’ telomere length for the senescence curves of the est1Δ rad52Δ survivor assays (n = 2 each). As published previously, the telomere length in est1Δ rad52Δ is slightly longer than in est1Δ cells. All telomere lengths were calculated by subtracting the amount of sub-telomeric DNA from the total length of the PCR product, giving the length of the TG1-3 tract exclusively. All graphs display mean and SEM.
(A) Cartoon depicting the Mph1 protein. The D209RE210R mutations alter the DEAH motif. (B) Western Blotting confirming the expression of MPH1-HA and mph1D209RE210R-HA in est1Δcells after about 25 population doublings. Proteins of two different clones (Figure 2D) were extracted. Ponceau staining serves as loading control. (C) Genomic DNA of the senescence curve presented in Figure 3A was blotted on a nylon membrane and hybridized with a DIG- labeled oligo-G-probe under native conditions (top panel) or denaturing conditions (bottom panel). Conversely to what has been observed in Figure 3D and E for the G- rich overhang, there was no accumulation of ssDNA of the C-rich strand detected. (D) Quantification of ssDNA on the C-strand (D) and normalization by the amount of total denatured DNA.
(A) Cartoon depicting the DEAH and Helicase of Mph1 and the respective positions of the point mutations used in this study. K113Q lies within the ATPase domain, D209 and E210 are residues of the DEAH helicase motif. (B) Overexpression of wild-type and mph1 point mutations in cdc13-1 under semi-permissive conditions. Only the mph1 D209RE210R shows a reduced toxicity in the cdc13-1 background. (C) Overexpression of mph1 D209RE210R is the least toxic in a cdc13-1 mph1Δ background at semipermissive temperature. (D) Western Blot showing that HA-tagged wildtype and mutant Mph1 are expressed at similar levels in a cdc13-1 mph1Δ background. Ponceau staining serves as loading control.
(A) Cartoon depicting the Mph1 protein lacking the C-terminal amino acids responsible for binding to RPA. (B) Western blotting confirming the expression of the mph1-ΔCter-HA mutant protein in an est1Δ background after about 25 population doublings. Ponceau staining serves as loading control. (C) The mph1-ΔCter-HA mutant protein is expressed in a cdc13-1 mutant strain. (D) Serial dilutions of strains grown for 5 days to stationary phase were spotted onto synthetic medium containing either glucose or galactose. MPH1 and EXO1 were constitutively expressed from a GPD-promoter containing plasmid. Galactose-induced inactivation of the centromere on chromosome 6 allows cells containing a telomere end-to-end fusion to grow stably, rather than suffering from genomic instability .
We appreciate the reagents sent by Jim Haber (Brandeis University, USA), Brad Johnson (University of Pennsylvania, USA) and Susan Gasser (FMI Basel, Switzerland) for the BIR, GC and NHEJ assays. Thanks to Charlie Boone (University of Toronto, Canada), Stephane Marcand (CNRS Fontenay-aux-roses, France) and Brad Johnson for temperature-sensitive alleles and plasmids. We thank Martina Dees for technical support and the rest of the Luke Lab for input throughout the study.
Conceived and designed the experiments: SLG BL. Performed the experiments: SLG. Analyzed the data: SLG BL. Contributed reagents/materials/analysis tools: SLG BL. Wrote the paper: SLG BL.
- 1. Bertuch AA, Lundblad V (2006) The maintenance and masking of chromosome termini. Curr Opin Cell Biol 18: 247–253.AA BertuchV. Lundblad2006The maintenance and masking of chromosome termini.Curr Opin Cell Biol18247253
- 2. Blackburn EH (2001) Switching and signaling at the telomere. Cell 106: 661–673.EH Blackburn2001Switching and signaling at the telomere.Cell106661673
- 3. Lundblad V (2002) Telomere maintenance without telomerase. Oncogene 21: 522–531.V. Lundblad2002Telomere maintenance without telomerase.Oncogene21522531
- 4. Cesare AJ, Reddel RR (2010) Alternative lengthening of telomeres: models, mechanisms and implications. Nat Rev Genet 11: 319–330.AJ CesareRR Reddel2010Alternative lengthening of telomeres: models, mechanisms and implications.Nat Rev Genet11319330
- 5. Muntoni A, Reddel RR (2005) The first molecular details of ALT in human tumor cells. Hum Mol Genet 14 Spec No. 2: R191–196.A. MuntoniRR Reddel2005The first molecular details of ALT in human tumor cells. Hum Mol Genet 14 Spec No.2R191196
- 6. Lydeard JR, Jain S, Yamaguchi M, Haber JE (2007) Break-induced replication and telomerase-independent telomere maintenance require Pol32. Nature 448: 820–823.JR LydeardS. JainM. YamaguchiJE Haber2007Break-induced replication and telomerase-independent telomere maintenance require Pol32.Nature448820823
- 7. Llorente B, Smith CE, Symington LS (2008) Break-induced replication: what is it and what is it for? Cell Cycle 7: 859–864.B. LlorenteCE SmithLS Symington2008Break-induced replication: what is it and what is it for?Cell Cycle7859864
- 8. McEachern MJ, Haber JE (2006) Break-induced replication and recombinational telomere elongation in yeast. Annu Rev Biochem 75: 111–135.MJ McEachernJE Haber2006Break-induced replication and recombinational telomere elongation in yeast.Annu Rev Biochem75111135
- 9. Lydeard JR, Lipkin-Moore Z, Jain S, Eapen VV, Haber JESgs1 and exo1 redundantly inhibit break-induced replication and de novo telomere addition at broken chromosome ends. PLoS Genet 6: e1000973.JR LydeardZ. Lipkin-MooreS. JainVV EapenJE HaberSgs1 and exo1 redundantly inhibit break-induced replication and de novo telomere addition at broken chromosome ends.PLoS Genet6e1000973
- 10. Whitby MC (2010) The FANCM family of DNA helicases/translocases. DNA Repair (Amst) 9: 224–236.MC Whitby2010The FANCM family of DNA helicases/translocases.DNA Repair (Amst)9224236
- 11. Tischkowitz MD, Hodgson SV (2003) Fanconi anaemia. J Med Genet 40: 1–10.MD TischkowitzSV Hodgson2003Fanconi anaemia.J Med Genet40110
- 12. Kao WH, Riker AI, Kushwaha DS, Ng K, Enkemann SA, et al. (2011) Upregulation of fanconi anemia DNA repair genes in melanoma compared with non-melanoma skin cancer. J Invest Dermatol 131: 2139–2142.WH KaoAI RikerDS KushwahaK. NgSA Enkemann2011Upregulation of fanconi anemia DNA repair genes in melanoma compared with non-melanoma skin cancer.J Invest Dermatol13121392142
- 13. Chen CC, Taniguchi T, D’Andrea A (2007) The Fanconi anemia (FA) pathway confers glioma resistance to DNA alkylating agents. J Mol Med (Berl) 85: 497–509.CC ChenT. TaniguchiA. D’Andrea2007The Fanconi anemia (FA) pathway confers glioma resistance to DNA alkylating agents.J Mol Med (Berl)85497509
- 14. Hazlehurst LA, Enkemann SA, Beam CA, Argilagos RF, Painter J, et al. (2003) Genotypic and phenotypic comparisons of de novo and acquired melphalan resistance in an isogenic multiple myeloma cell line model. Cancer Res 63: 7900–7906.LA HazlehurstSA EnkemannCA BeamRF ArgilagosJ. Painter2003Genotypic and phenotypic comparisons of de novo and acquired melphalan resistance in an isogenic multiple myeloma cell line model.Cancer Res6379007906
- 15. Schurer KA, Rudolph C, Ulrich HD, Kramer W (2004) Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from Homologous recombination, but not from postreplicative repair. Genetics 166: 1673–1686.KA SchurerC. RudolphHD UlrichW. Kramer2004Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from Homologous recombination, but not from postreplicative repair.Genetics16616731686
- 16. Banerjee S, Smith S, Oum JH, Liaw HJ, Hwang JY, et al. (2008) Mph1p promotes gross chromosomal rearrangement through partial inhibition of homologous recombination. J Cell Biol 181: 1083–1093.S. BanerjeeS. SmithJH OumHJ LiawJY Hwang2008Mph1p promotes gross chromosomal rearrangement through partial inhibition of homologous recombination.J Cell Biol18110831093
- 17. Scheller J, Schurer A, Rudolph C, Hettwer S, Kramer W (2000) MPH1, a yeast gene encoding a DEAH protein, plays a role in protection of the genome from spontaneous and chemically induced damage. Genetics 155: 1069–1081.J. SchellerA. SchurerC. RudolphS. HettwerW. Kramer2000MPH1, a yeast gene encoding a DEAH protein, plays a role in protection of the genome from spontaneous and chemically induced damage.Genetics15510691081
- 18. Prakash R, Satory D, Dray E, Papusha A, Scheller J, et al. (2009) Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Genes Dev 23: 67–79.R. PrakashD. SatoryE. DrayA. PapushaJ. Scheller2009Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.Genes Dev236779
- 19. van Attikum H, Fritsch O, Hohn B, Gasser SM (2004) Recruitment of the INO80 complex by H2A phosphorylation links ATP-dependent chromatin remodeling with DNA double-strand break repair. Cell 119: 777–788.H. van AttikumO. FritschB. HohnSM Gasser2004Recruitment of the INO80 complex by H2A phosphorylation links ATP-dependent chromatin remodeling with DNA double-strand break repair.Cell119777788
- 20. Pobiega S, Marcand S (2010) Dicentric breakage at telomere fusions. Genes Dev 24: 720–733.S. PobiegaS. Marcand2010Dicentric breakage at telomere fusions.Genes Dev24720733
- 21. Evans SK, Lundblad V (1999) Est1 and Cdc13 as comediators of telomerase access. Science 286: 117–120.SK EvansV. Lundblad1999Est1 and Cdc13 as comediators of telomerase access.Science286117120
- 22. Lundblad V, Blackburn EH (1993) An alternative pathway for yeast telomere maintenance rescues est1- senescence. Cell 73: 347–360.V. LundbladEH Blackburn1993An alternative pathway for yeast telomere maintenance rescues est1- senescence.Cell73347360
- 23. Abdallah P, Luciano P, Runge KW, Lisby M, Geli V, et al. (2009) A two-step model for senescence triggered by a single critically short telomere. Nat Cell Biol 11: 988–993.P. AbdallahP. LucianoKW RungeM. LisbyV. Geli2009A two-step model for senescence triggered by a single critically short telomere.Nat Cell Biol11988993
- 24. Deshpande AM, Ivanova IG, Raykov V, Xue Y, Maringele L (2011) Polymerase epsilon is required to maintain replicative senescence. Mol Cell Biol 31: 1637–1645.AM DeshpandeIG IvanovaV. RaykovY. XueL. Maringele2011Polymerase epsilon is required to maintain replicative senescence.Mol Cell Biol3116371645
- 25. Garvik B, Carson M, Hartwell L (1995) Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint. Mol Cell Biol 15: 6128–6138.B. GarvikM. CarsonL. Hartwell1995Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint.Mol Cell Biol1561286138
- 26. Luke-Glaser S, Luke B, Grossi S, Constantinou A (2010) FANCM regulates DNA chain elongation and is stabilized by S-phase checkpoint signalling. EMBO J 29: 795–805.S. Luke-GlaserB. LukeS. GrossiA. Constantinou2010FANCM regulates DNA chain elongation and is stabilized by S-phase checkpoint signalling.EMBO J29795805
- 27. Kee Y, Kim JM, D’Andrea AD (2009) Regulated degradation of FANCM in the Fanconi anemia pathway during mitosis. Genes Dev 23: 555–560.Y. KeeJM KimAD D’Andrea2009Regulated degradation of FANCM in the Fanconi anemia pathway during mitosis.Genes Dev23555560
- 28. Pace P, Mosedale G, Hodskinson MR, Rosado IV, Sivasubramaniam M, et al. (2010) Ku70 corrupts DNA repair in the absence of the Fanconi anemia pathway. Science 329: 219–223.P. PaceG. MosedaleMR HodskinsonIV RosadoM. Sivasubramaniam2010Ku70 corrupts DNA repair in the absence of the Fanconi anemia pathway.Science329219223
- 29. Adamo A, Collis SJ, Adelman CA, Silva N, Horejsi Z, et al. Preventing nonhomologous end joining suppresses DNA repair defects of Fanconi anemia. Mol Cell 39: 25–35.Adamo A, Collis SJ, Adelman CA, Silva N, Horejsi Z, et al. Preventing nonhomologous end joining suppresses DNA repair defects of Fanconi anemia.Mol Cell392535
- 30. de Lange T (2009) How telomeres solve the end-protection problem. Science 326: 948–952.T. de Lange2009How telomeres solve the end-protection problem.Science326948952
- 31. Griffith JD, Comeau L, Rosenfield S, Stansel RM, Bianchi A, et al. (1999) Mammalian telomeres end in a large duplex loop. Cell 97: 503–514.JD GriffithL. ComeauS. RosenfieldRM StanselA. Bianchi1999Mammalian telomeres end in a large duplex loop.Cell97503514
- 32. de Bruin D, Kantrow SM, Liberatore RA, Zakian VA (2000) Telomere folding is required for the stable maintenance of telomere position effects in yeast. Mol Cell Biol 20: 7991–8000.D. de BruinSM KantrowRA LiberatoreVA Zakian2000Telomere folding is required for the stable maintenance of telomere position effects in yeast.Mol Cell Biol2079918000
- 33. Strahl-Bolsinger S, Hecht A, Luo K, Grunstein M (1997) SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast. Genes Dev 11: 83–93.S. Strahl-BolsingerA. HechtK. LuoM. Grunstein1997SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast.Genes Dev118393
- 34. Pryde FE, Louis EJ (1999) Limitations of silencing at native yeast telomeres. EMBO J 18: 2538–2550.FE PrydeEJ Louis1999Limitations of silencing at native yeast telomeres.EMBO J1825382550
- 35. de Bruin D, Zaman Z, Liberatore RA, Ptashne M (2001) Telomere looping permits gene activation by a downstream UAS in yeast. Nature 409: 109–113.D. de BruinZ. ZamanRA LiberatoreM. Ptashne2001Telomere looping permits gene activation by a downstream UAS in yeast.Nature409109113
- 36. Vannier JB, Pavicic-Kaltenbrunner V, Petalcorin MI, Ding H, Boulton SJ (2012) RTEL1 Dismantles T Loops and Counteracts Telomeric G4-DNA to Maintain Telomere Integrity. Cell 149: 795–806.JB VannierV. Pavicic-KaltenbrunnerMI PetalcorinH. DingSJ Boulton2012RTEL1 Dismantles T Loops and Counteracts Telomeric G4-DNA to Maintain Telomere Integrity.Cell149795806
- 37. Gari K, Decaillet C, Delannoy M, Wu L, Constantinou A (2008) Remodeling of DNA replication structures by the branch point translocase FANCM. Proc Natl Acad Sci U S A 105: 16107–16112.K. GariC. DecailletM. DelannoyL. WuA. Constantinou2008Remodeling of DNA replication structures by the branch point translocase FANCM.Proc Natl Acad Sci U S A1051610716112
- 38. Kang YH, Kang MJ, Kim JH, Lee CH, Cho IT, et al. (2009) The MPH1 gene of Saccharomyces cerevisiae functions in Okazaki fragment processing. J Biol Chem 284: 10376–10386.YH KangMJ KangJH KimCH LeeIT Cho2009The MPH1 gene of Saccharomyces cerevisiae functions in Okazaki fragment processing.J Biol Chem2841037610386
- 39. Collis SJ, Ciccia A, Deans AJ, Horejsi Z, Martin JS, et al. (2008) FANCM and FAAP24 function in ATR-mediated checkpoint signaling independently of the fanconi anemia core complex. Mol Cell 32: 313–324.SJ CollisA. CicciaAJ DeansZ. HorejsiJS Martin2008FANCM and FAAP24 function in ATR-mediated checkpoint signaling independently of the fanconi anemia core complex.Mol Cell32313324
- 40. Xue Y, Li Y, Guo R, Ling C, Wang W (2008) FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair. Hum Mol Genet 17: 1641–1652.Y. XueY. LiR. GuoC. LingW. Wang2008FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair.Hum Mol Genet1716411652
- 41. Wu X, Sandhu S, Nabi Z, Ding H (2012) Generation of a mouse model for studying the role of upregulated RTEL1 activity in tumorigenesis. Transgenic Res. Epub ahead of print. X. WuS. SandhuZ. NabiH. Ding2012Generation of a mouse model for studying the role of upregulated RTEL1 activity in tumorigenesis. Transgenic Res.Epub ahead of print