Deletions of IKAROS (IKZF1) frequently occur in B-cell precursor acute lymphoblastic leukemia (B-ALL) but the mechanisms by which they influence pathogenesis are unclear. To address this issue, a cohort of 144 adult B-ALL patients (106 BCR-ABL1-positive and 38 B-ALL negative for known molecular rearrangements) was screened for IKZF1 deletions by single nucleotide polymorphism (SNP) arrays; a sub-cohort of these patients (44%) was then analyzed for gene expression profiling.
Total or partial deletions of IKZF1 were more frequent in BCR-ABL1-positive than in BCR-ABL1-negative B-ALL cases (75% vs 58%, respectively, p = 0.04). Comparison of the gene expression signatures of patients carrying IKZF1 deletion vs those without showed a unique signature featured by down-regulation of B-cell lineage and DNA repair genes and up-regulation of genes involved in cell cycle, JAK-STAT signalling and stem cell self-renewal. Through chromatin immunoprecipitation and luciferase reporter assays we corroborated these findings both in vivo and in vitro, showing that Ikaros deleted isoforms lacked the ability to directly regulate a large group of the genes in the signature, such as IGLL1, BLK, EBF1, MSH2, BUB3, ETV6, YES1, CDKN1A (p21), CDKN2C (p18) and MCL1.
Here we identified and validated for the first time molecular pathways specifically controlled by IKZF1, shedding light into IKZF1 role in B-ALL pathogenesis.
Citation: Iacobucci I, Iraci N, Messina M, Lonetti A, Chiaretti S, Valli E, et al. (2012) IKAROS Deletions Dictate a Unique Gene Expression Signature in Patients with Adult B-Cell Acute Lymphoblastic Leukemia. PLoS ONE 7(7): e40934. doi:10.1371/journal.pone.0040934
Editor: Felipe Prosper, Clinica Universidad de Navarra, Spain
Received: January 15, 2012; Accepted: June 15, 2012; Published: July 25, 2012
Copyright: © 2012 Iacobucci et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The funding that we received for this study came from: AIRC IG 2010 – Grant Proposal 2010, Fondazione Del Monte di Bologna e Ravenna, FIRB 2006, Ateneo RFO grants, Project of integrated program (PIO), and Programma di Ricerca Regione - Università 2007 - 2009.The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Based on cytogenetic and molecular analyses, B-cell precursor acute lymphoblastic leukemia (B-ALL) carries hyperdiploidy, hypodiploidy, translocations t(12;21)[ETV6-RUNX1], t(1;19)[TCF3-PBX1], t(9;22)[BCR-ABL1] and rearrangement of the MLL gene. The frequencies of these abnormalities vary among children and adults and their detection is important in diagnosis and risk stratification , . In addition, approximately one quarter of B-ALL cases, defined as “B-NEG” throughout the text, lack recurring cytogenetic and molecular abnormalities, and the genetic basis of these cases is unknown , , . Recently, genome-wide approaches to study DNA copy number abnormalities and loss of heterozygosity (LOH) events led to impressive progresses and revolutionized the knowledge of the genetic basis of ALL , , , . In B-ALL a high frequency of genetic abnormalities in key pathways, including lymphoid differentiation, cell cycle regulation, tumor suppression, and drug responsiveness, have been identified , . Among these abnormalities, deletions of IKZF1 gene, encoding the transcription factor Ikaros, were found in about 28% of pediatric BCR-ABL1 negative ALL cases and in more than 80% of adult BCR-ABL1 positive cases , . Ikaros is required for the earliest stages of lymphoid lineage commitment and for tumor suppression in mouse , , . In many cases, IKZF1 deletions lead to the expression of dominant-negative Ikaros splice variants (e.g. Ik6) that are characterized by loss of N-terminal zinc finger DNA binding domains, and retention of the C-terminal dimerization domain , . IKZF1 deletions were significantly associated with an increased relapse rate and adverse events and were correlated with poor outcome in both pediatric and adult patients with BCR-ABL1-positive ALL , , . However, the precise mechanism(s) by which Ikaros alterations contribute to leukemogenesis and worse prognosis have not yet been clearly defined. To investigate this aspect, we correlated the genomic status of the IKZF1 gene in a cohort of adult B-ALL patients (BCR-ABL1 positive and B-ALL negative for known molecular rearrangements, here named B-NEG, cases) with transcription profiles. Furthermore, through chromatin immunoprecipitation and luciferase assays, we have defined how full-length and deleted forms of IKZF1 can affect gene expression. Our results show that independently from BCR-ABL1, the loss of IKZF1 function dictates a unique expression signature characterized by down-regulation of B-cell lineage and DNA repair genes and up-regulation of genes involved in cell-cycle progression, JAK/STAT signalling and stem cell self-renewal, shedding light on the molecular mechanisms that may underlie the leukemogenic potential of Ikaros deletions.
IKZF1 Deletions Occur in 71% of B-precursor Adult ALL (B-NEG and BCR-ABL1+)
SNP arrays analyses revealed that among the 144 patients tested (Table 1), deletions of IKZF1 occurred in 22 out of 38 (58%) B-NEG ALL cases and in 80 out of 106 (75%) adult BCR-ABL1+ cases, indicating that IKZF1 deletion is more frequent in the BCR-ABL1+ ALL subgroup (p = 0.04). When the whole cohort of adult B- ALL patients (B-NEG and BCR-ABL1+) was considered, IKZF1 deletion occurred in 102 (71%) cases. Deletions were predominantly mono-allelic (89% vs 11%) and showed a variable pattern of extension over the IKZF1 locus (Figure 1). Among patients carrying deletions in IKZF1, 19 (19%) showed a complete deletion of the locus, whereas in 83 (81%) patients only a subgroup of exons or the genomic region immediately upstream of IKZF1 was deleted. In particular, in 50 patients (49%) there was a deletion of the coding exons 4 through 7, causing expression of the dominant-negative isoform Ik6, lacking the DNA binding domain. In 27 cases (26%) we identified the loss of exons 2 through 7, producing an Ikaros isoform lacking the translation start site. In the remaining cases, the deletion either included exon 8 or the promoter and exon 1 regions, contributing to IKZF1 haploinsufficiency. We did not observe differences in the occurrence of different types of deletions across BCR-ABL1+ and B-NEG cases.
Schematic representation of the IKZF1 gene on chromosome 7p12, with coding exons (ex) in gray and the first non-coding exon in white. N-terminal zinc-fingers (F1–F4) show DNA-binding activity and C-terminal ones (F5–F6) mediate dimerization of the protein. Black segment reports indicate the genomic extent of the IKZF1 deletions of the most common deletions. Percentage is reported for each type of deletion.
Gene Expression Analysis Revealed a Unique Signature Associated with IKZF1 Deletions
To evaluate whether cases harbouring IKZF1 alterations display a peculiar gene expression profile, we performed two supervised analyses on gene expression data from 62 B-ALL patients (30 BCR-ABL+ and 32 B-NEG ALL). IKZF1 deletion was equally distributed in this experimental setting among B-ALL subtypes with a rate of 53% and 47% in BCR-ABL1+ and B-NEG ALL, respectively (Table S1). Since some cases (8 BCR-ABL1+ and 3 B-NEG) lost IKZF1 as the result of the monosomy of chromosome 7, where IKZF1 is located (7p12), we decided to divide our cohort in 3 groups: A) cases with IKZF1-deletions (n = 31), B) cases without IKZF1 deletions (n = 20) and C) cases with loss of IKZF1 due to monosomy 7 (n = 11). Gene expression profiles of these three groups were compared by Analysis of Variance (ANOVA). Results showed that 1602 probes, corresponding to 1130 genes, are differentially expressed and grouped patients in three sub-clusters with distinct expression profiles. It should be noted that one sub-cluster was constituted by almost all cases (10 out of 11) with monosomy of chromosome 7 (Figure 2A): this result depended on the fact that 84.6% of down-modulated genes are indeed located on chromosome 7, thus complicating and probably masking the genuine expression signature mediated by IKZF1 alone. Notably, a clear clustering of monosomy 7 cases was also evident by unsupervised clustering (data not shown). Therefore, to overcome this issue, we specifically focused on patients with or without deletions in the IKZF1 locus. By t-test analysis 684 probes, corresponding to 422 genes, were found differentially expressed between IKZF1-deletion positive and negative patients: 128 genes were down-modulated and 294 genes up-regulated in IKZF1-deletion positive (median FDR: 13%) (Tables S2 and S3, respectively) (Figure 2B).
A) Hierarchical clustering of 62 B-ALL cases based on 1602 probes resulting from ANOVA. Cases with chromosome 7 monosomy branched together in a unique homogeneous subcluster which was included in a cluster comprising the majority of IKZF1-deletion positive samples; B) Gene expression profile of 51 B-ALL adult samples (excluding chromosome 7 monosomy cases), based on 684 probes identified by t-test. Each row represents a probe set, each column represents a single ALL sample. Bottom, color scale indicating the relative levels of expression: dark blue, the lowest levels of expression; red, highest levels of expression.
Deletions of Ikaros Deregulate Multiple Genes Involved in Specific B-cell Intracellular Pathways
To identify peculiar critical pathways affected by Ikaros deletions, the 294 up-regulated and 128 down-regulated genes were analyzed in terms of biological function using the Metacore pathway-mapping software and the DAVID software. When considering the top 10 statistically down-regulated networks (Figure S1A), we detected several genes involved in B-cell differentiation pathway (BLK, BTK, IGLL1, CD22, PLCG2, MAP3K1, VPREB1) and, to a lesser extent, in apoptosis and cell cycle (BUB3, BID, 14-3-3 protein beta/alpha). Functional analysis, performed using the DAVID software, confirmed these findings and, in addition, highlighted the DNA repair gene category (MSH2, MSH6, UBE2V2) as significantly down-modulated.
Among the up-regulated genes, a substantial number appeared to be involved in cell cycle and apoptosis regulation (STK17B, SERPINB9, CDKN1A (p21), CDKN2C (p18), FLT1, LATS2, JUND, MCL1, etc). Importantly, the JAK-STAT signalling pathway (YES1, CISH, SOCS1, SOCS3, SOCS5, STAT3), DNA damage (GADD45A, GADD45B, REL), hematopoietic stem cell (HSC)-affiliated genes (CDKN1A, SOCS2, SOCS3, ALDH2, ETV6 and MLLT3) (Figure S1B) as well as the early primed erythroid factor KLF9 and the late myeloid gene ID2, indicating their premature induction, were also found up-regulated.
Furthermore, we also evaluated the expression of a set of genes previously shown to play a role in leukemogenesis, namely, EBF1, CD200, PAX5, RB1, CDKN2A, LEF1, BTG1 and RAG . This analysis highlighted an up-regulation of CD200 in IKZF1-deleted patients and a down-modulation of RAG and EBF1 (data not shown). This is in agreement with previous data showing that Ikaros regulates IGHV recombination .
To understand whether IKZF1 deletions may affect intracellular signalling we compared the activation status of the JAK-STAT pathway between primary leukemia cells obtained from four patients with, and two without, IKZF1 deletion. As previously demonstrated in murine pro-B lymphocytes , the expression of isoforms derived from deletion was associated with increased phosphorylation of STAT5 (Figure 3A) and significantly induced mRNA expression of the downstream anti-apoptotic Bcl-xl (Figure 3B). Since in children with B-ALL and IKZF1 deletions, JAK2 mutations have been reported to constitutively activate JAK2 , we performed a mutational screening in order to assess whether this was also the case in our cohort. Overall 17 BCR-ABL1-positive and 16 B-NEG negative ALL cases were analyzed for JAK2 mutations. These cases included six patients on which western blot analysis for pSTAT5 was performed. No mutations were found, except for the silent D820 and L830 mutations identified each in single cases and for the missense E444K and D489N, identified both in one BCR-ABL1 negative patient. The latter mutations were outside the pseudokinase domain of JAK2.
Western Blot analysis of STAT5 in IKZF1 deleted and wild-type leukemia primary cells (A) showed an increased phosphorylation of STAT5 in cells with Ikaros deletion. This was correlated with an increased production of Bcl-xl (B) as determined by RQ-PCR. (C) Western Blot analysis showed a marked increased phosphorylation of histone H2A.X in leukemia cells with Ikaros deletion compared to normal IKZF1 cells. Abbreviations: LC indicates leukemia cells. β-ACTIN was used as a loading control of the WB.
Moreover, to investigate whether Ikaros deletions correlate with accumulation of DNA damage, as suggested by gene expression signature, we examined the phosphorylation of histone H2A.X (γ-H2A.X) as an indicator of the cellular response to DNA damage . Primary leukemia cells with Ikaros deletion showed a marked increase in the basal phosphorylation of H2A.X when compared to leukemia cells with wild-type Ikaros, suggesting that Ikaros deletions co-exist in cells with the propensity to genome instability (Figure 3C).
IKZF1 Deleted Isoforms Lack the Ability to Directly Regulate a Large Group of Target Genes in vivo
Thereafter, to determine whether IKZF1 is directly involved in the regulation of putative target genes identified in gene expression analysis, the promoter regions mapping around their transcriptional start sites were scanned for the presence of the conserved IKZF1 binding site (GGGAA) . We found several potential Ikaros binding sites in the promoters of many deregulated genes, such as IGLL1, BLK, EBF1, BUB3, MSH2, MSH6, BTK, VPREB1, CD22, MCL1, ETV6, YES1, CDKN1A (p21) and CDKN2C (p18). Assessment of direct association of Ikaros isoforms to tested promoters was determined by applying cross-linking chromatin immunoprecipitation (ChIP) assay to two different cell lines: SD-1 and BV-173 expressing IKZF1 full-length protein or the IK6 deleted isoform, respectively . A pre-immune serum was used as a negative control to determine the baseline of the nonspecific background.
As shown in Figure 4, the promoter regions of the selected genes, such as IGLL1, BLK, EBF1, BUB3, MSH2, MCL1, ETV6, YES1, CDKN1A (p21) and CDKN2C (p18) were bound in vivo only by IKZF1 full-length protein in SD-1 cell line, but not by Ik6 mutant in BV-173 cells. A distal DNA region (Amplicon A) for each gene, lacking IKZF1 consensus sites, was tested in ChIP as a negative control.
Analysis of down-regulated (A) and up-regulated (B) genes in IKZF1-deletion positive cases through chromatin Immunoprecipitation assay (ChIP). The experiments were performed in SD-1 and in BV-173 cell lines, expressing full-length Ikaros transcription factor and deleted isoform IK6, respectively. Results represent the average of three independent experiments in which each region was amplified by qPCR in triplicate. Standard error is indicated. Promoter diagram: bent arrow, transcription start site; black arrow, Ikaros binding sites; open boxes, amplicons indicated with a capital letter; chromosome and coordinates (bp) are also given.
Although transcriptionally affected by IKZF1 deletions, the promoters of MSH6, BTK, VPREB1 and CD22, and genes were not bound by IKZF1, neither in SD-1 nor in BV-173 cell lines (Figure S2), thence suggesting that these genes may be “secondary targets” of Ikaros transcriptional activity. A similar result was also obtained when ChIP was applied to primary cells of two B-ALL patients: one carrying a full-length IKZF1 and the second one carrying the deleted Ik6 isoform (Figure S3).
To confirm that Ik1 full-length is required to activate or repress transcription, we performed experiments using Luciferase reporter constructs carrying the promoter regions of three different genes illustrative of those whose promoters were bound by Ik1 in ChIP experiments. Specifically, we chose to analyze EBF1 and MSH2 which are down-regulated in IKZF1-deletion positive cases and MCL1 which is up-regulated. Recombinant reporters [EBF1(−900/+100); MSH2(−154/+484); MCL1(−661/+255)] were transiently co-transfected in HEK-293 cells along with either a Ik6 or a Ik1 expression vector and luciferase activity was quantified. Expression levels of the Ik1 and Ik6 exogenous proteins in transfected cells was quantified by Western Blotting (Figure 5A).
A) Western blotting of Ik1 and Ik6 proteins transiently expressed by respective vectors when transfected into HEK-293 cells. B) Left: schematic representation of the promoters cloned upstream of the pGL3basic-Luciferase vector. Right: relative luciferase activity of the reporters determined by co-transfection with either an Ik6 or an Ik1 expression vector as compared to that obtained with the empty vector which was arbitrarily set to 1. Results represent the average of three independent experiments.
As expected, results show that the IKZF1 full-length protein can both positively (EBF1 and MSH2) and negatively (MCL1) modulate the luciferase activity of the luc-reporters, whereas the deleted Ik6 isoform cannot (Figure 5B).
Overall, these data support a model in which Ik6 has lost the ability to bind target genes and to properly control their transcription profile.
While different studies have established that Ikaros alterations have a pivotal role in the leukemogenesis of BCR-ABL1 positive and negative ALL , , , the precise mechanisms by which they promote leukemogenesis or cooperate with known molecular rearrangements (e.g. BCR-ABL1) remain to be elucidated. Here, we took advantage of high-resolution SNP array, transcriptional profiling and chromatin immunoprecipitation approaches to explore these mechanisms. In physiologic conditions, Ikaros is expressed in multipotent, self-renewing hematopoietic stem cells (HSCs) as well as in their committed progenitors and its expression increases during lymphocytes development , . In mice, heterozygous disruption of Ikaros leads to development of T-cell malignancies , , , whereas homozygous inactivation is responsible for severe lymphoid cell defects with abnormal thymopoiesis and perturbed myelopoeisis  , , . Recently , Ikaros was described as a key coordinator of the lymphoid-myeloid genetic network that is responsible for activation of lymphoid signatures in the HSCs and for repression of lineage inappropriate signatures downstream of HSCs. Perturbations of this tightly regulated network may clearly alter hematopoiesis development and promote leukemogenesis.
Collectively, in the largest cohort of adult B-ALL patients (106 BCR-ABL1 positive and 38 B-NEG) reported so far we detected deletions of IKZF1 gene in 71% B-ALL cases with a variable pattern of breakpoints. In the majority of cases, alterations were found to encode transcripts lacking the DNA binding domain acting as dominant negative isoforms, or transcripts lacking the translation start site contributing to Ikaros haploinsufficiency.
We further investigated the effects of these alterations, identifying a unique gene expression profile for patients with Ikaros deletions unrelated to BCR-ABL1 rearrangement but specifically associated with Ikaros genomic status. This signature was characterized by down-regulation of genes required for B-cell lineage development and DNA repair upon response to DNA damage and up-regulation of genes involved in cell cycle/apoptosis, JAK-STAT signalling and stem cell self-renewal. Although our data were only partially consistent with those reported by Mullighan et al. in children with high-risk ALL  and with recent gene expression profiling studies that have identified the new BCR-ABL1-like subtype , here for the first time we showed by ChIP analysis that Ikaros can bind in vivo the promoters of many genes differentially expressed between IKZF1 deletion and IKZF1 wild-type cases. Among these, we formally demonstrated, through luciferase reporter assay, that EBF1, MSH2 and MCL1, involved in B-cell development, DNA repair and inhibition of apoptosis, respectively, are novel identified Ikaros targets. EBF1 is a chief determinant of B cell fate, inducing expression of B cell–specific genes and IGH gene recombination and also represses the myeloid developmental program . In IKZF1−/− multipotent progenitors, EBF1 ectopic expression was shown to rescue B-cell development and redirect, at low frequency, myeloid progenitors toward the B-cell lineage. This successful ‘rescue’ suggested that Ikaros may be required for the developmental induction of EBF1 but this hypothesis was thereafter not addressed . Here, beyond demonstrating that EBF1 is an Ikaros target, we also provided strong lines of evidence that Ikaros deletion directly reduces activation of EBF1 in leukemia cells contributing to an extensive block of B-cell differentiation.
We also demonstrated that Ikaros can bind the promoter region of MSH2 indicating a novel role for Ikaros as a direct regulator of DNA repair. Ikaros is expressed in multipotent, self-renewing HSC  in which the repair of potential DNA damage is crucial to avoid stem cell depletion. Reese and colleagues showed that the HSC of MSH2 null mice possess an enhanced capacity to survive the exposure to the methylating agent, temozolomide (TMZ), a compound that causes DNA damage requiring mismatch repair mechanisms . Although additional experiments will be required to formally demonstrate the role of Ikaros in maintaining genomic integrity, the accumulation of DNA damage we found in cells with Ikaros deletions, together with our ChIP/luciferase results, lead us to hypothesize that loss of Ikaros could contribute to increase genomic instability in leukemia stem cells. Moreover, the up-regulation of genes involved in inhibition of apoptosis (e.g. MCL1) may promote the survival of leukemia cells with accumulated DNA damage and their expansion as suggested by an up-regulation of genes involved in cell cycle progression (AHR, AHR, TUBB2A, RAPGEF3, SERTAD1, FLT1). Noteworthy, among the genes up-regulated we found an over-expression of CDKN1A (p21) which has been recently  demonstrated to be indispensable for maintaining self-renewal of leukemia stem cells and avoiding their exhaustion. Based on our findings from SNP array, gene expression profile and ChIP/luciferase results we propose the following model: in physiologic condition Ikaros is expressed in the multipotent, self-renewing hematopoietic stem cells  where it is responsible for i) activation of a cascade of lymphoid genes, allowing lineage differentiation; ii) tight control of cell cycle progression and iii) DNA repair. Downstream of the hematopoietic stem cells, Ikaros restricts the multilineage potential of lymphoid and myeloid progenitors by repressing lineage-inappropriate genetic programs including hematopoietic stem cell–specific genes. Deletions result in the majority of cases in the production of isoforms unable to bind the DNA, which determine a block of differentiation, increased proliferation , continuous self-renewal and accumulation of DNA damage.
In conclusion, our findings shed light on a new subgroup of B-ALL, including BCR-ABL1 positive and B-NEG patients, characterized by a unique signature dependent on Ikaros deletion. The prognostic impact of Ikaros deletions, that translates in an increased relapse rate and an overall poor outcome in both BCR-ABL1 positive patients and BCR-ABL1 negative patients (Tables S4 and S5) , , , ,  highlights the importance to perform a screening of deletions at diagnosis. Finally, a comprehensive understanding of the molecular consequences of Ikaros deletion may help in identifying novel treatment options for B-cell–progenitor ALL patients.
Materials and Methods
This study was approved by GIMEMA AL Working Party (Gruppo Italiano Malattie EMatologiche Maligne dell’Adulto Acute Leukemia). All patients gave informed consent to blood collection and biologic analyses, in agreement with the Declaration of Helsinki. Overall, we collected peripheral blood or bone marrow samples from a cohort of 144 newly diagnosed adult ALL patients (106 BCR-ABL1-positive, 38 negative for the major molecular rearrangements, B-NEG) enrolled from 1996 to 2008 in different clinical trials of GIMEMA AL Working Party (LAL 0496, LAL 2000, LAL 0201-B and LAL 1205) and institutional protocols. Detailed information on the clinical trials of GIMEMA AL Working Party is reported in the Table S6. The main clinical and biological characteristics of the patients are shown in Table 1. The human BCR-ABL1-positive leukemia cell lines SD1 and BV-173 were also included in the analysis. They were obtained from DMSZ (Deutshe Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany) and maintained in culture following the DMSZ recommendations.
Single Nucleotide Polymorphism (SNP) Array Analysis
Samples were genotyped with GeneChip® Human Mapping 250 K NspI and Genome-Wide Human SNP 6.0 arrays (Affymetrix) according to the manufacturer’s instructions and as previously described , . Copy number aberrations in IKZF1 were scored using the Hidden Markov Model and the segmentation approach available within the Partek Genomic Suite (Partek Inc) software package as well as by visual inspection. Copy number aberrations were also analyzed and eventually confirmed using Genotyping Console 3.0 (Affymetrix). SNP array data have been previously deposited according to .
IKZF1 Genomic Quantitative and Qualitative PCR
In order to confirm the IKZF1 deletions detected by SNP array and to define the extension of alterations, IKZF1 genomic quantitative PCR (Q-PCR) of all IKZF1 coding exons was performed as described by Mullighan et al.  using a 7900 Real-Time PCR system and 7900 System Software (Applied Biosystems). Characterization of breakpoints occurring in IKZF1 was carried out by long-range PCR experiments, as previously described .
RNA Extraction and Gene Expression Profiling Experiments
Sixty-two, including 32 B-NEG and 30 BCR-ABL1+ B-ALL, out of 144 samples analyzed by SNP, were also studied for gene expression profiling (44%). HGU133 Plus 2.0 gene chips (Affymetrix) were used to perform gene expression profile experiments. Thawed or freshly isolated cells were homogenized and total RNA was extracted using the RNeasy mini kit (Qiagen). The quality of total RNA was checked by agarose gel electrophoresis and RNA concentration was determined by measuring the absorbance at 260 nm; for all samples, the 260/280 ratio was >1.8, as required for microarray analysis. Array preparation was performed as described . Gene expression data have been previously deposited in the context of the MILe Study  with GEO accession number GSE13204.
Gene Expression Data Analysis
Oligonucleotide microarray and gene expression data analysis were performed using the dChip software (www.dchip.org) , which utilizes an invariant set normalization method where the array with median overall intensity is chosen as the baseline for normalization. Model based expressions were computed for each array and probe set using only perfect match probes.
ANOVA with p-value ≤0.01 was performed to compare the 3 subgroups based on IKZF1 genomic status as evaluated by SNP: IKZF1-deletion positive (i.e. cases displaying any focal deletion in IKZF1), IKZF1-negative cases (cases without IKZF1 deletions), cases with chromosome 7 monosomy. A t-test with p≤0.05 and fold change difference ≥1.5 was then used to compare only IKZF1-deletion positive with IKZF1-deletion negative cases. False discovery rate (FDR) was performed over 250 permutations. A functional annotation analysis was applied to the list of selected genes using the DAVID software (http://david.abcc.ncifcrf.gov)  based on Gene Ontology terms. Moreover, a functional enrichment analysis was performed using the Metacore software (GeneGo Inc., www.genego.com) .
Western Blot Analysis
Cells were lysed in RIPA buffer supplemented with phosphatase inhibitors. Western blots were performed according to Invitrogen procedures for NuPAGE Novex Bis-Tris Gel Electrophoresis system (Invitrogen), using 100 µg of whole-cell extracts. The antibodies used were: pStat5 and γ-H2AX antibodies (Cell Signaling Technology); Stat5 (Millipore); β-ACTIN (Sigma-Aldrich).
Quantitative PCR analysis for Bcl-xl
The expression of Bcl-xl was examined using the following primers: F1∶5′-TGCGTGGAAAGCGTAGACAAGG-3′ and R1∶5′-CAAGGCTCTAGGTGGTCATTCAGG-3′ and a Sybr Green PCR master Mix (Applied Biosystems, Foster City, CA) on a 7900 Real-Time PCR system. The quantitative PCR thermal protocol consisted of: 50°C for 2 minutes, followed by 95°C for 10 minutes, then 40 cycles of 95°C for 1 minute and 60°C for 1 minute. GAPDH was used as control gene and the Stratagene's Universal Human Reference RNA was used as calibrator (Stratagene). The expression of Bcl-xl was assessed in 12 B-ALL patients with Ikaros deletion and in 10 B-ALL patients with wild-type Ikaros.
JAK2 Mutation Screening
JAK2 mutation screening was performed on cDNA samples from 33 patients (17 BCR-ABL1 positive and 16 BCR-ABL1 negative cases) using the following pair primers: F1∶5′- AACTGTCATGGCCCAATTTC-3′ and R1∶5′- TGGCACATACATTCCCATGA-3′ and F2∶5′- TTGCTAAACAGTTGGCATGG-3′ and R2: CACATCTCCACACTCCCAAA-3′. cDNA was amplified with 2U of FastStart Taq DNA Polymerase (Roche Diagnostics), 0.8 mM dNTPs, 1 mM MgCl2, and 0.2 M forward and reverse primers in 25 µl reaction volumes. PCR products were purified using QIAquick PCR purification kit (Qiagen) and then directly sequenced using an ABI PRISM 3730 automated DNA sequencer (Applied Biosystems) and a Big Dye Terminator DNA sequencing kit (Applied Biosystems). All sequence variations were detected by comparison using the BLAST software tool (www.ncbi.nlm.nih.gov/BLAST/) to reference genome sequence data obtained from the UCSC browser (http://genome.ucsc.edu/cgi-bin/hgBlat?command=start; February 2009 release).
In vivo Chromatin Immunoprecipitation (ChIP) Assay
2×107 SD1 and BV173 cell lines and 2×107 primary cultured cells from two patients with ALL were used for each immunoprecipitation. Genomic DNA was sonicated to obtain fragments in the range of 200–500 bp. The lysate was pre-cleared by incubating it with 50 µl of Immobilized Protein A (Pierce) for 15 minutes in the cold room at constant rotation. 5 µg of specific antibody were added and incubated O/N in the cold room at constant rotation. DNA fragments were amplified by Real-time quantitative PCR (RQ-PCR) using the iQ™ SYBR Green Supermix and the iQCycler thermocycler (Bio-Rad). 1/50 of the resuspended IP-DNA, was used in each PCR reaction. Antibodies employed in this study were: IgG sc-2027 and Ikaros sc-13039 (Santa Cruz Biotechnology). Specific pairs of primers used for quantitative ChIP are listed in Table S7.
The EBF1, MSH2 and MCL1 promoters were amplified by PCR and cloned into the pGL3-basic vector (Promega). The Renilla–TK vector was used as an internal control. HEK-293 cells were transiently transfected using Lipofectamine2000 (Invitrogen) according to manufacturer’s instructions. The luciferase activity was analyzed 48 h after transfection according to Dual Luciferase Assay manufacturer’s instructions (Promega).
Biological process networks overrepresented in the list of genes down-regulated (A) and up-regulated (B) in B-ALL patients with IKZF1 deletion compared to wild-type patients. Enrichment analysis was performed using metacore-mapping software (GeneGo Inc.). Log of p-values represent the probability of a given number of genes being associated with each network by chance.
Ikaros does not associate with promoters of the MSH6, BTK, VPREB1 and CD22 genes. ChIP assay was performed in SD-1 and in BV-173 cell lines, expressing full-length Ikaros transcription factor and deleted isoform IK6, respectively. Results represent the average of three independent experiments in which each region was amplified by qPCR in triplicate. Standard error is indicated. Promoter diagram: bent arrow, transcription start site; black arrow, Ikaros binding sites; open boxes, amplicons indicated with a capital letter; chromosome and coordinates (bp) are also given.
A large group of target promoters are not bound in vivo by deleted Ikaros in cells from a B-ALL patient. ChIP assay was performed as described in SD-1 and in BV-173 cell lines.
Characteristics of the patients analyzed by gene expression profiling.
List of the down-regulated genes in IKZF1 -deleted B-ALL patients (p<0.05).
List of the up-regulated genes in IKZF1 -deleted B-ALL patients (p<0.05).
Treatment outcome and results of therapy related to IKZF1 loss in univariate analysis. Abbreviations: mths (months), wt (wild-type).
IKZF1 deletion and other clinical relevant factors for predicting Disease Free Survival (multivariate analysis). Abbreviations: WBC (white blood cells).
Description of GIMEMA clinical trials enrolling patients who were analyzed in this study.
Specific pairs of primers used for quantitative ChIP analysis. Different amplicons were analyzed for each gene. Abbreviations: F (forward), R (reverse).
Authors of GIMEMA contributing to the study.
We specially thank the AIL (Associazione Italiana contro le Leucemie - linfomi e myeloma), Serena Formica (University of Bologna) and Annalisa Astolfi (University of Bologna) for help in performing SNP arrays, Luciana Impera for performing FISH analysis, Barbara Lama, Sarah Parisi and Maria Chiara Abbenante (University of Bologna) for collecting clinical data. We also acknowledge all the people who take care of the patients involved in the GIMEMA studies (see Appendix S1).
Conceived and designed the experiments: II GM. Performed the experiments: II NI M. Messina SC EV AL EO AF VG SD. Analyzed the data: II NI CTS CP AV SS FP RF MB M. Messina M. Müschen GP. Contributed reagents/materials/analysis tools: FP MV. Wrote the paper: II NI M. Messina SC.
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