Within vertebrates, major sex determining genes can differ among taxa and even within species. In zebrafish (Danio rerio), neither heteromorphic sex chromosomes nor single sex determination genes of large effect, like Sry in mammals, have yet been identified. Furthermore, environmental factors can influence zebrafish sex determination. Although progress has been made in understanding zebrafish gonad differentiation (e.g. the influence of germ cells on gonad fate), the primary genetic basis of zebrafish sex determination remains poorly understood. To identify genetic loci associated with sex, we analyzed F2 offspring of reciprocal crosses between Oregon *AB and Nadia (NA) wild-type zebrafish stocks. Genome-wide linkage analysis, using more than 5,000 sequence-based polymorphic restriction site associated (RAD-tag) markers and population genomic analysis of more than 30,000 single nucleotide polymorphisms in our *ABxNA crosses revealed a sex-associated locus on the end of the long arm of chr-4 for both cross families, and an additional locus in the middle of chr-3 in one cross family. Additional sequencing showed that two SNPs in dmrt1 previously suggested to be functional candidates for sex determination in a cross of ABxIndia wild-type zebrafish, are not associated with sex in our AB fish. Our data show that sex determination in zebrafish is polygenic and that different genes may influence sex determination in different strains or that different genes become more important under different environmental conditions. The association of the end of chr-4 with sex is remarkable because, unique in the karyotype, this chromosome arm shares features with known sex chromosomes: it is highly heterochromatic, repetitive, late replicating, and has reduced recombination. Our results reveal that chr-4 has functional and structural properties expected of a sex chromosome.
Citation: Anderson JL, Rodríguez Marí A, Braasch I, Amores A, Hohenlohe P, Batzel P, et al. (2012) Multiple Sex-Associated Regions and a Putative Sex Chromosome in Zebrafish Revealed by RAD Mapping and Population Genomics. PLoS ONE 7(7): e40701. doi:10.1371/journal.pone.0040701
Editor: Laszlo Orban, Temasek Life Sciences Laboratory, Singapore
Received: March 14, 2012; Accepted: June 12, 2012; Published: July 9, 2012
Copyright: © 2012 Anderson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research was funded by National Institutes of Health (NIH) grant R01GM085318 (to JHP), NIH grant P01HD22486 (to JHP), a Feodor-Lynen-Fellowship of the Alexander von Humboldt Foundation (to IB), and a grant from the Volkswagen Foundation (to IB). The research was also supported by a Major Research Instrumentation grant from the National Science Foundation, Office of Cyber Infrastructure, “MRI-R2: Acquisition of an Applied Computational Instrument for Scientific Synthesis (ACISS)”, Grant #: OCI-0960354. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The process of sex determination specifies an animal’s sex and activates developmental pathways that cause gonads to differentiate into ovaries or testes. The mechanisms of animal sex determination, however, are remarkably diverse. Some animals utilize genetic sex determining mechanisms (GSD) while others rely on environmental cues for sex determination (ESD). GSD systems can be either chromosomal, and involve a master sex-determining gene on a sex chromosome, such as Sry in mammals , or can be polygenic and involve several genes on multiple chromosomes (e.g. ). In ESD, the environment plays a decisive role, such as temperature in turtles and alligators –. GSD and ESD can interact in some species; for example, in Japanese medaka (Oryzias latipes), which has an XX/XY genetic system, high temperatures can cause female-to-male sex reversal –.
Zebrafish (Danio rerio) provides a model to understand vertebrate development, physiology, and behavior, but the primary genetic mechanisms that determine zebrafish sex remain elusive. Zebrafish do not have obvious sexually dimorphic chromosomes –. Specific cross designs can yield predictable sex ratios – and genetic loci associated with sex have been identified , supporting a role for GSD in this species. Environmental factors such as hypoxia, high temperatures, and crowding can also influence sex ratio in zebrafish , –, as expected for ESD. Thus, both GSD and ESD appear to function in this species. Because sex ratio can vary widely between crosses, stocks, and generations, sex determination in zebrafish seems to be rather plastic and easily pushed in one direction or the other by genetic or environmental factors . We do not understand, however, the primary genetic mechanisms that underlie these events nor how the environment might perturb them.
Although the genes and primary genetic mechanisms that initiate sex determination in zebrafish remain unknown, downstream genes in the sex determination pathway appear to be highly conserved with other taxa and a general model of sex determination in these fish has been proposed . As juveniles, all zebrafish develop gonads containing immature oocytes  and express genes appropriate for the development of both testes (e.g., amh, sox9a, cyp11b) and ovaries (cyp19a1a and foxl2) –. Around the fourth week after fertilization, gonads extinguish expression of one or the other set of genes. In some juveniles, oocytes die and the fish become males, but in others, oocytes survive and the fish develop as females , . Interestingly, in zebrafish lacking germ cells, somatic cells of the gonad express testis-specific genes, and animals develop exclusively as males , –. The presence of germ cells, however, is insufficient to feminize the gonads, suggesting that the presence of oocytes progressing through meiosis is essential to support female development in zebrafish , , , . This shows that post-recombination meiotic zebrafish oocytes provide an as yet unidentified signal to the soma that supports female development . We propose that in zebrafish, genetic and environmental factors act directly or indirectly to regulate the strength of the meiotic oocyte-derived factor . Outstanding questions remain: what are those factors and how do they work?
A better understanding of the molecular genetic basis for sex determination in a species like zebrafish with a delicately balanced sex determining mechanism might lend insight into the increasing rates of human reproductive diseases, such as testicular dysgenesis syndrome (TDS) and polycystic ovary syndrome (PCOS) , , and provide practical benefits for studies that employ this widely used animal model. To help better understand the genetic mechanisms underlying zebrafish sex determination, we surveyed for sex-associated loci in zebrafish.
In principle, studies comparing the genomes of zebrafish males and females should identify genetic elements that are associated with sex. Quantitative trait locus (QTL) mapping approaches in various fish species have identified chromosome regions containing loci with effects on sex determination , –. A recent investigation that mapped sex in a cross between the AB and IN (India) wild-type zebrafish stocks (the ABxIN cross)  identified loci on chr-5 and chr-16 [loci here referred to as sex-associated region chr-5 (sar5) and sar16]; furthermore, the ABxIN study detected variants in two nearby candidate genes, dmrt1 in sar5, a paralog of which is associated with sex determination in medaka, chickens, and frogs , , , , and cyp21a2 in sar16 (steroid 21-hydroxylase), some mutant alleles of which cause virilization in humans . Additional studies are necessary, however, because sex determination mechanisms can vary even among strains or populations of fish, for example, in poeciliid fish and cichlids , , and the same may be true in zebrafish.
Here, we analyzed the F2 offspring of reciprocal crosses between Oregon *AB (star AB) and NA (Nadia) wild-type zebrafish stocks to map sex using over 5,000 polymorphic Illumina sequencing based RAD-tag markers – that we genotyped in 231 fish distributed in two families. We observed sex-associated loci on the end of the long arm of chr-4 for both of two *ABxNA families and in the middle of chr-3 for one of the two families, but did not recover the loci found to segregate with sex in the ABxIN cross . Population genomic analyses of genetic differentiation between sexes at more than 30,000 single nucleotide polymorphisms (SNPs) per cross family confirmed the mapping results. Furthermore, two SNPs in dmrt1 previously proposed as functional candidates  failed to associate with sex in our fish. We conclude that sex determination in zebrafish is likely to be polygenic and genes that influence sex development may be strain or environment specific. Furthermore, the sex-associated locus on chr-4 (sar4) occupies the only chromosome arm in the zebrafish karyotype that is late-replicating, repeat-rich, and heterochromatic , , , properties that characterize mature sex chromosomes in many other species. This result raises the possibility that chr-4 is a sex chromosome in zebrafish.
Materials and Methods
The University of Oregon Institutional Animal Care and Use Committee approved all animal work (Animal Welfare Assurance Number A-3009-01, IACUC protocol # 11-07).
Stocks and Cross Design
Nadia and *AB (star AB) fish were obtained from the zebrafish facility at the University of Oregon. *AB stocks were derived in 1991–1992 from George Streisinger’s original AB stocks  that had been maintained at the University of Oregon since the 1970s (http://zfin.org/action/genotype/genotype-detail?zdbID=ZDB-FISH-960809-7). Nadia stocks originated from wild populations of zebrafish north of Kolkata (Calcutta), India, and have been in culture at the University of Oregon since 1999 (http://zfin.org/action/genotype/genotype-detail?zdbID=ZDB-GENO-030115-2). All fish were reared as previously described . The University of Oregon Institutional Animal Care and Use Committee approved all animal work (Animal Welfare Assurance Number A-3009-01, IACUC protocol # 11-07).
Reciprocal single-pair matings of *AB and Nadia fish generated F1 individuals. Full-sib single-pair matings of F1 fish produced F2 individuals used for mapping. Two F2 families were used, one from the initial F0 cross between a Nadia female and *AB male (Family A) and the other from the reciprocal F0 cross between a *AB female and a Nadia male (Family B). Family A consisted of 86 female and 103 male F2 siblings. Family B consisted of 56 female and 96 male F2 siblings. The sex of F0 and F1 individuals was assessed by the examination of sexually dimorphic external characters and the types of gametes each fish produced. The sex of F2 individuals was assessed by external characters and confirmed by the dissection and direct observation of the gonads, classifying them as either ovaries or testes. Fish that could not be confidently sexed after gonad dissection were excluded from further analysis.
RAD Genotyping–RAD (Restriction site Associated DNA) libraries were created as previously described , . In brief, genomic DNA was extracted from muscle using a Qiagen DNeasy Blood and Tissue Kit and digested with high-fidelity SbfI restriction enzyme (New England Biolabs). Barcoded adapters were ligated to each sample. Barcodes were 6 nucleotides (nt) long and differed from each other by at least 2 nt to facilitate unambiguous identification of each sample following sequencing. Samples were pooled, sheared, and the 200–500 base pair (bp) size fraction purified from an agarose gel after electrophoresis. Following ligation of a second adaptor, fragments containing both adapters were PCR amplified (12 cycles) and the 200–500 bp size fraction isolated as above. RAD libraries were sequenced on an Illumina HiSeq 2000 using 100 bp single-end reads. We RAD-sequenced all F0 and F1 fish used to produce Families A and B, 167 F2 siblings from Family A, and 128 F2 siblings from Family B. Sequences are available online at the NCBI Sequence Read Archive (http://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi; accession number SRA049620.1).
Sequence data were processed using the process_radtags component of the Stacks pipeline (http://creskolab.uoregon.edu/stacks/) , which removes reads of low quality and bins data by barcode. Sequences were then aligned to the zebrafish genome (Zv9) using Bowtie 0.12.5 . Sequences that aligned to more than one place in the genome or that aligned to unassembled scaffolds were excluded from further analysis. RAD-tag markers were identified in the F1 parental fish from each cross and scored in F2 individuals in Stacks. Conservatively, a minimum read-depth of 20× coverage per marker per individual was required for a sequence to be identified as a RAD-tag in Stacks. Genotypes, which were formatted for map type CP (outbred full-sib family), were then exported from Stacks and used for genetic map construction.
Genetic Map Construction, Linkage Analysis, and Recombination Rates
Genetic maps for Family A and Family B were generated in JoinMap 4.1 (Wageningen, The Netherlands), using the Kosambi mapping function and a multipoint maximum likelihood mapping algorithm for cross type CP . Maternal and paternal markers were used to construct separate male- and female-specific linkage maps for each chromosome to facilitate identification of improbable double recombination events and incorrect marker order. Genotypes associated with double recombination events, as identified by JoinMap, were verified or corrected in Stacks (as in ). Marker order was initially based on positional data from Zv9 and manually refined. New male- and female-specific maps were generated using corrected genotypes and refined marker orders and analysis was reiterated until suspicious genotypes and double recombination events were minimized. Consensus maps were then generated for each chromosome. Markers with significant segregation distortion (P<0.01), unlinked at LOD<6, or with excessive missing data were excluded from analysis. Individuals with read depth lower than 20 reads for more than 20% of the markers for Family A and more than 30% for Family B were also excluded. Residual improbable double recombination events were identified and treated as above. Genotype data were exported from JoinMap and recoded for analysis as a phase-known 4-way cross in R/qtl using a custom python script (Supporting Text S1). Linkage analysis to identify loci associated with sex was performed in R/qtl  using interval mapping and a model appropriate for the analysis of a binary trait. Statistical significance for linkage to sex was determined using a permutation test (10,000 permutations).
We calculated sex-specific recombination rates in Family A from marker positions in the final male- and female-specific maps (cM, above) and marker position in Zv9 (Mb) using a sliding window of 3 markers (moving 1 marker per estimate).
In previous work, numbers were assigned to zebrafish linkage groups (LGs) based on genetic length , ; these LGs are called ‘chromosomes’ in the Zv9 version of the zebrafish genome at Ensembl, and we abbreviate them here as ‘chr’. Subsequently, LGs were assigned to physical chromosomes  that cytogeneticists had previously numbered based on physical length , , –. In this paper, we use the designation ‘chr’ to mean ‘Linkage Group’ rather than cytogenetically identified physical chromosomes, in agreement with zebrafish nomenclature conventions. Thus, chr-4 herein (Linkage Group 4) is cytogenetic Chromosome-3 and chr-3 (Linkage Group 3) is cytogenetic Chromosome-4 .
SNP Genome Scan
We also tested the association of sex with genotypes at individual single nucleotide polymorphisms (SNPs) across the genome in the F2 individuals. Starting with the sequence data aligned to the reference genome with Bowtie (from above), we assigned a diploid genotype at each nucleotide position in each individual using a modification of the maximum likelihood genotyping method of . This method assigns a diploid genotype if a likelihood ratio test between the two most likely genotypes is significant (here at á = 0.01), and does not assign a genotype if the test is not significant, based on a multinomial sampling model of read counts for alternative nucleotides. This method implicitly results in a minimum threshold for sequencing depth, depending on the ratio of read counts for alternative nucleotides, because low-coverage sites will not result in a significant likelihood ratio test. Thus we did not explicitly filter for sequencing depth in this analysis. We modified this method to include a bounded uniform prior distribution on the per-nucleotide sequencing error rate å from 0.001 to 0.1; that is, the likelihood for each possible genotype was calculated as the maximum only within this range of å (see equation 1 in ). This approach reduces the error rate of assigning a homozygous genotype when the true genotype is heterozygous but with uneven or biased read counts, as occurs in Illumina-based RAD sequencing (Hohenlohe, unpublished data). Within each family, we filtered out SNPs that were genotyped at fewer than 20 male or 20 female individuals. We then conducted a G-test for significant genetic differentiation between males and females at each SNP , and we calculated the false discovery rate within each family using the algorithm of Benjamini & Hochberg .
Partial Sequencing of dmrt1
The partial sequencing of dmrt1 from one male and one female in the ABxIN cross identified two potentially functional sex-specific SNPs: dbSNP ss184964113 and ss184964140 . To determine whether these SNPs are associated with sex in our AB fish stocks, we sequenced from 12 males and 12 females, 628 bp of dmrt1, including exon-5 and part of the 3¢UTR and both potentially functional sex-specific SNPs using forward primer 5¢CCCAAACCAGATTACGCTCTGGCA and reverse primer 5¢TGCATCATGAAGGTCGCGGG. Heterozygous base positions were identified by Geneious v5.5  and verified by manual inspection.
Genomic Analysis of chr-4
To address the question of whether chr-4 has, and is unique in having, characteristics of a sex chromosome, we downloaded the locations of genes in the zebrafish genome assembly Zv9 from Ensembl65 using Biomart (http://www.ensembl.org/biomart/martview/). Protein coding genes were analyzed according to their Ensembl entries. Repeat elements for Zv9 were downloaded from the Table Browser of the UCSC Genome Browser (http://genome.ucsc.edu/cgi-bin/hgTables?org=zebrafish). Genomic distribution of genes and other elements was visualized using the Integrative Genomics Viewer (IGV 2.0.34) .
Chromosome spreads were obtained from fibroblast tissue cultures from caudal fin clips , , from 4 male and 4 female individuals. For replication banding, 5-bromodeoxyuridine (BrdU) (30 µg/ml final concentration) was added to the cultures 6–8 hours before harvesting the cells. Colchicine (0.02 µg/ml final concentration) was added 1–2 hours before harvesting the cells. Collected cells were treated in a hypotonic solution of 0.075 M KCl for 20 minutes at room temperature. The cell suspension was fixed by four washes in 3∶1 methanol-acetic acid. Cells were spread onto cleaned slides and air-dried. For replication banding, we immersed dry slides for 30 min in Hoechst 33258 (1 µg/ml), rinsed in distilled water, and covered them with a thin layer of 2× SSC before exposure to a 15 W Sylvania G15T8 germicidal lamp at a distance of 10 cm for 60 min. After incubating slides in 0.5×SSC at 60°C for 60 min, we rinsed slides in distilled water and stained in 4% Giemsa for 20 min. We investigated more than 10 different metaphases per individual.
Sequences, SNPs, and Genetic Maps
To identify loci linked to sex, we constructed F2 mapping populations from two distantly related zebrafish strains. We sequenced SbfI-associated RAD-tags in 167 offspring from Family A (*AB grandsire and Nadia granddam) and 128 offspring from Family B (the reciprocal cross, Nadia grandsire and *AB granddam) (Table 1). RAD-tag sequencing identified about 46,000 tags, each 100 nucleotides long, associated with SbfI cut sites genome wide (Table 1). Approximately 37% of all sequenced tags contained at least one SNP in the F1 parents for each cross; of these, 5180 tags were genotyped in a minimum of 90% of individuals in Family A. In Family B, 6357 tags were genotyped in at least 70% of the F2 offspring. These polymorphic markers were exported from Stacks for use in genetic map construction. Following the exclusion of markers and individuals due to missing data and segregation distortion criteria (see methods), genetic maps of length 2346 cM (centiMorgans) for Family A (145 individuals) and 2333 cM for Family B (86 individuals) were constructed based on 4796 or 5794 markers respectively (Table 1, Figure S1). The genetic and physical positions of mapped markers can be found in Tables S1 and S2. Overall, fewer than 10% of the polymorphic markers were excluded during genetic map construction. In Family B, nearly all markers on chromosome 14 exhibited significant segregation distortion but were retained for analysis.
Linkage analysis identified one sex-associated region on chr-4 in both cross families and an additional locus on chr-3 linked to sex in Family B (Figure 1). Hereafter, we refer to the chr-4 locus as sar4 for sex-associated region-4, and the chr-3 locus as sar3. In Family A, sar4 peaks at 92 cM [LOD 28.78, marker ID29464 near Zv9 physical position 4∶61,176,889 bp (base pairs); 1.5 LOD drop interval from 91.0 cM (marker ID29449 near 4∶60,837,953 bp) to the end of chr-4 at 62,094,675 bp]. In Family-B, the sar4 peak is at 98.4 cM [LOD 7.98, marker ID32525 near physical position 4∶61,422,807 bp, 1.5 LOD drop interval from 96.7 cM (marker ID32485 near physical location 4∶60,837,953 bp) to the end of chr-4 at 4∶62,094,675 bp]. In Family B, sar3 resides at 45.3 cM [LOD 7.87, marker ID29552 near physical position 3∶20,676,406 bp; 1.5 LOD drop interval from 44.1 cM to 49.9 cM (physical positions marker ID29370 near physical position 3∶15,234,176 to marker ID29830 at 3∶29,193,306 bp)]. These peaks exceed genome-wide significance for linkage to sex in their respective crosses (Figure 1).
(A) Family A, *AB grandsire. (B) Family B, NA grandsire. Sex is associated with a locus on chr-4 in both families, and a locus on chr-3 in Family B. LOD scores are plotted in order of marker position in cM. Significance thresholds were determined by permutation testing and are indicated by horizontal lines (red = 5%, gray = 10%).
The marker at the peak of sar4 in Family A contains one SNP (C/G). All 70 individuals homozygous for the C allele at this SNP were male (one or both C alleles inherited from the homozygous C/C grandsire and the other C inherited from the heterozygous G/C granddam), whereas 84% (63/75) of C/G heterozygotes were female, the G allele having passed from the NA granddam through the heterozygous C/G dam and the C inherited either from the grandsire or the granddam (Figure 2A, Table S3). While it might be tempting to interpret this relationship between haplotype and phenotype as evidence of a ZW/ZZ mode of sex determination, it must be noted that not all adjacent markers, which were also statistically associated with sex in this analysis, support this interpretation. Thus, until the causative genetic factor in sar4 is identified or more closely mapped, it is premature to ascribe an XX/XY or ZW/ZZ role to sar4 (likewise for sar3 below).
(A) In Family A, all individuals homozygous for the C allele at Marker ID29464 chr-4∶61,176,889 bp were male and 84% of individuals heterozygous at this marker were female. (B) In Family B, loci on chr-3 and -4 appear to interact to influence sex (marker ID32525 chr-4∶61,422,807bp; marker ID29552 chr-3∶20,676,406bp). Individuals homozygous for grandsire-derived alleles at the chr-4 locus developed as males independent of their chr-3 genotype and animals homozygous for granddam-derived alleles were all or nearly all female without regard to their chr-3 genotype. On the other hand, individuals that were homozygous for the grandsire-derived alleles at the chr-3 locus were male independent of their genotype at the chr-4 locus.
In Family B, relationships between haplotype and sex were more complex: some combinations of haplotypes on chr-3 and chr-4 were found in both males and females but others were found exclusively in either males or females (Figure 2B, Table S4). All 16 individuals with the grandsire-derived ACA/CCG alleles for sar4 were male independent of their genotype at sar3, and all 18 fish with the granddam-derived genotype CCG/CTA or homozygous for CCG (one copy originating from each grandparent), were female if they were heterozygous AG/CC at sar3. These results show that multiple genes can influence sex determination in zebrafish and that genes affecting sex determination may differ among strains.
SNP Genome Scan
A total of 36,860 SNPs in Family A and 36,370 SNPs in Family B were genotyped in 20 or more individuals of each sex in each family. We tested association of genotype with sex at each of these SNPs using a G-test , corrected for false discovery rate within each family . Information about the significant sex-associated SNPs can be found in Tables S5 and S6. Individual SNPs significantly associated with sex (Figure 3) closely matched genomic regions sar3 and sar4 identified in the linkage analysis (Figure 1). In Family A, SNPs on chr-4 at physical locations 62,076,809 bp and 62,076,876 bp exhibited the strongest association; additional highly significant SNPs (−log10p>40) clustered between 60,613,763 bp and the end of chr-4. In Family B, highly significant SNPs (−log10p>10) occurred between physical positions on chr-4 at 60,762,591 bp and 61,934,267 bp. On chr-3, an additional broad cluster of significant SNPs in Family B matched the peak observed in the linkage analysis. In addition, a small cluster of SNPs representing just two RAD-tags in both families was significantly associated with sex in a 24 kb region on chr-14, between physical locations 37,844,124 bp and 37,868,461 bp. These two markers fell in two adjacent predicted genes (CABZ01075275.1 and CABZ01075273.1) that Ensembl scores as having 226 paralogs, most of which are on the long arm of chr-4, many in sar4; these markers were excluded from the linkage analysis above because they were genotyped to criterion depth in too few animals. About 40% of BAC clones that uniquely hybridize to the long (q) arm of chr-4 (chr-4q) were assembled onto chr-14 in the Zv6 version of the zebrafish genome ; in Zv9, most of these BAC end sequences were assembled onto chr-4q (data not shown). Therefore, it is possible that the sex associated chr-14 SNPs we identify here may also belong on chr-4q like the other originally misassembled BACs.
(A) Family A. (B) Family B. Individual SNPs were tested for their non-random association with male or female phenotypes. Graphs plot the -log10p-value of a G-test across the genome and show peaks at the sar4 and sar3 locations. Red lines show the 0.1 percent false discovery rate within each family. Peaks in chr-14 are in genes that are members of a major multigene family in sar4, and may be mis-localized in the current genome assembly.
Partial Sequencing of dmrt1
In the ABxIN cross, dmrt1 from one male and one female fish, each homozygous for alternative male- and female-associated alleles, was resequenced, and found to differ by 162 SNPs, 28 indels, and two simple tandem repeats . Two of these SNPs within dmrt1 were suggested to be functional candidates . To test whether these SNPs were associated with sex in our AB fish, we sequenced a 628 bp region of dmrt1 in 12 female and 12 male AB fish. All 24 fish in our study, including both males and females, were homozygous for C at ss184964113 (a C/A polymorphism causing a nonsynonymous (D259E) substitution in Dmrt1) . At ss184964140, an A/G SNP in what is thought to be the cis-regulatory motif of the 3¢UTR , , our 24 AB fish were either homozygous for A, or A/G heterozygotes, but the genotype at this SNP was not associated with sex (Fisher’s exact test P = 0.21). We observed polymorphisms at 7 additional positions (Text S2), but none were associated with sex. Rather, both males and females were either homozygous throughout this region, or heterozygous at all eight polymorphic sites. We conclude that the candidate functional SNPs in the ABxIN cross  are not associated with sex in our AB stocks and thus may not function as sex determinants in all strains of zebrafish.
Recombination Across the Genome
A major feature of sex determining chromosomal regions is the suppression of recombination –. To investigate genome-wide recombination rates, we performed a sliding window analysis of the ratio of genetic map distance in cM per Mb of the Zv9 assembly of the zebrafish genome for both the male genetic map and the female map. Results showed first, that in general, the recombination rate is reduced in males compared to females (Figure 4A, B), thus corroborating earlier results for a genetic map based on male meiosis . Second, results showed that in general, for both males and females, recombination rates tended to be higher towards the telomeres on most chromosomes, also confirming prior findings for a sex-averaged map . Third, the comparison of the rates of recombination across chr-4 in males and females showed that recombination was greatly reduced in males around the sex-associated region at distal chr-4q (Figure 4C, D). This type of recombination suppression is expected of chromosome regions containing sex-determining factors –.
(A) Female-specific recombination genome-wide. (B) Male-specific recombination genome-wide. (C) Female-specific recombination across chr-4. (D) Male-specific recombination across chr-4. Recombination rates were measured in a sliding window comparing the map distance between markers to the genomic location on the Zv9 assembly of the zebrafish genome. Recombination is generally elevated at telomeres but is reduced in males at sar4. For ease of visualization, recombination rates of excess of 35 cM/Mb at the top of the graph are indicated by *. In the female, these rates equal 88, 49, 67, 52, and 108 cM/Mb from right to left. In the male, these rates equal 94, 50, and 39 cM/Mb.
The Physical Nature of chr-4q
To investigate whether chr-4 is unique in the genome in possessing features commonly found in sex chromosomes, we compared the distribution of various genetic elements across the genome (Figure 5, Table 2). First, chr-4q is the only arm in the zebrafish genome that is heterochromatic. Chr-4 (as designated at Ensembl and originally defined as Linkage Group-4 , ) is the third largest cytogenetic chromosome in zebrafish  and is used as a standard for cytogenetic comparisons because of its unique heterochromatic long (right) arm (chr-4q) –, . The heterochromatic region on chr-4 in genome assembly Zv9 begins at about 28 Mb and contains sar4 at about 61–62 Mb. This region comprises about 2.26% of the entire genome assembly. Zebrafish centromeres have been mapped , –, showing that the microsatellite marker Z10280 (GenBank accession G39774) lies near the centromere of chr-4 at 20.3 Mb in Zv9 (Figure 5B). Some of the repetitive elements of the heterochromatic region mentioned below extend into the euchromatic region at the end of chr-4q.
(A) Genome wide distribution (chr-1 to −25 across the top of the figure) of genetic elements enriched in the heterochromatic region of chr-4. Rows listed top to bottom: 5S RNA motif, micro RNAs (miRNAs), four different Ensembl multigene families (ENSFM), small nuclear RNAs (snRNAs), transfer RNAs (tRNAs), Kolobok-1 transposon, and pseudogenes. See Table 2 for specifics on these elements. (B) Within chr-4 (position in Mb listed across the top), enriched elements are found in the hetrochromatic region (green) delineated by the mir-430 cluster (light blue) at the centromeric side and sar-4 (red) near the telomere of the p arm. (C) Schematic of chr-4 with locations of the centromere, heterochromatic region and sar-4. (D) Replication banding of a cell cultured from a female zebrafish showing the late replication of chr-4q (arrows), unique among the 50 zebrafish chromosome arms. (E) Enlargement of the chr-4 pair; note heterochromatic chr-4q.
Chr-4q has accumulated numerous special genetic elements, including 5S RNA motifs, miRNA repeats (especially mir-430 see below), a number of repetitive gene families, snRNA genes, tRNA genes, the transposable element Kolobok-1, and pseudogenes. Cytogenetic studies showed that 5S rRNA probes hybridize to chr-4q – and this is confirmed by the distribution of the 5s rRNA sequence motif in Zv9, with a strong enrichment on chr-4q (Table 2, Figure 5A, B row 1). The centromere-proximal portion of the heterochromatic region includes a cluster of 55 members of the miRNA family mir-430 at about 28.0 Mb. Only one mir-430 gene is found outside this cluster, on chr-10 (Table 2, Figure 5A, B, row 2).
Chr-4q is enriched for multiple copies of genes belonging to several specific Ensembl protein families. Figure 5 A and B (rows 3–6) illustrate four gene families that have 189 to 436 copies spread throughout the genome, with substantial concentrations (28–65% of the copies) located in the heterochromatic region of chr-4q (Table 2). These include a zinc-finger fragment family and others of uncharacterized nature. Notably, chr-4q is enriched in additional multigene families with lower copy numbers (Table 2), such that these families are found nearly exclusively in the heterochromatic region of chr-4q.
Chr-4q contains a number of additional repetitive elements. Small nuclear RNAs (snRNAs) are enriched on chr-4 (Table 2, Figure 5A, B row 7). This enrichment is due specifically to the abundance of snRNAs U6 and U1. Nearly half of all tRNAs in the zebrafish genome are clustered in the heterochromatic region of chr-4q. (Table 2, Figure 5A, B row 8). While chr-4q is not enriched for transposons in general, it is enriched for copies of the transposon Kolobok 1, a member of the autonomous Kolobok transposon family (Figure 5A, B and S2) . Other members of the Kolobok family are not highly represented on chr-4 (Figure S2), but 14% of all Kolobok 1 copies in zebrafish are found within the approximately 34 Mb long heterochromatic region of chr-4q (Table 2).
The X-chromosomes of mammalian female somatic cells are late replicating . The incubation of zebrafish cells with bromodeoxyuridine (BrdU) revealed that chr-4q is also late replicating in zebrafish (Figure 5C,D). Results were consistent in all metaphases analyzed, and no major differences were observed between the male and female karyotypes. Figure 5 (C and D) illustrates a typical karyotype from a female individual.
We conclude that chr-4 possesses a number of properties generally associated with mature sex chromosomes, not the least of which is a sex-associated locus.
The primary genetic mechanisms that control gonad fate and determine sex in zebrafish remain poorly understood. This gap in our understanding persists despite several decades of intense investigation on zebrafish development and genetics. Developmental studies based on candidate genes show that the zebrafish sex determination pathway shares many genes in common with gonad developmental pathways in other vertebrate taxa. These approaches, however, have not yet identified the master genetic factors that initiate sex determination. To advance our understanding of sex determination in zebrafish, it is necessary to identify these factors. To help identify genes associated with sex in zebrafish, we genotyped 231 F2 offspring from reciprocal crosses between NA and *AB wild-type strains at more than 46,000 SbfI associated RAD-tags distributed throughout the genome, on average one every 37 kb and analyzed genotypes using both quantitative genetic and population genomic methods. Analyses converged on the identification of two genomic regions linked to sex. One of those regions, sar4, lies on the end of chr-4q and was linked to sex in both of our cross families (Figure 1, 3). The other sex-associated region, sar3, was in the middle of chr-3 and was identified in only one of the crosses (Figure 1, 3). These results suggest that sex determination in zebrafish is polygenic and that different genes may influence sex development in different strains and/or under different environmental conditions. Furthermore, the association of the end of chr-4q with sex in both cross families is of particular interest because chr-4 shares many physical features with known sex chromosomes.
Multiple Loci Influence Sex Determination in Zebrafish
In some taxa, including mammals and medaka, a single gene of large effect controls sex determination (Sry and Dmrt1by respectively). We found, however, that in one of the F2 families of our *ABxNA cross, more than one locus was associated with sex (Figure 1, 3, 6). The sex associated loci in the ABxIN cross ( loci referred to herein as sar5 and sar16), and classical breeding experiments in zebrafish  further support the polygenic nature of zebrafish sex determination. Polygenic sex determination mechanisms have been observed in several fish. For example, gilthead sea bream (Sparus aurata), red top zebra cichlid (Metriaclima pyrsonotus), and platyfish (Xiphophorus maculatus) all have polygenic sex determination systems , , .
The physical positions of genes with a potential influence on sex determination in zebrafish are marked in purple (see for names and locations the full list in Table S7). Red boxes indicate the 1.5 LOD drop intervals for sar3 and sar4 identified here in the *ABxNA cross and blue rectangles mark the 1.5 LOD droop intervals for sar5 and sar16 in the ABxIN cross . Dashed lines mark peaks of sex-associated loci identified herein (red) and by Bradley et al.  (blue). Black stars indicate genes identified as candidates for functional sex determination genes in zebrafish by Bradley et al. . The red star indicates hsd17b1. Upper and lower bounds of the surrounding boxes represent the 1.5 LOD drop intervals for each peak.
The genetic basis of sex determination in zebrafish appears to differ among strains or environmental conditions. We identified two loci associated with sex in zebrafish. The sar4 locus was associated with sex in both Family A (*AB grandsire) and Family B (NA grandsire). The sar3 locus, however, was sex-associated only in Family B. Two additional sex-associated loci were identified in the ABxIN cross (IN grandsire) . The three crosses studied to date differ in that grandsires (*AB, NA, IN) and granddams (NA, AB, and its derivative *AB, see methods) from different strains were used in each. Thus, one explanation for the observation of different sex-associated loci in these crosses is that zebrafish harbor intraspecific variation for genes influencing sex determination. Recent population genetic analyses show that wild populations of zebrafish are variable and divergent and that the AB laboratory strain differs markedly from outbred wild populations –. While both IN and NA originate from India, the IN strain originates from Darjeeling and the NA strain comes from Nadia north of Kolkata; thus, it is possible that these strains are genetically divergent. The AB strains used in the two different laboratories had a common origin in the initial AB strains established in Oregon by Streisinger and collaborators  and are separated by only a few tens of generations. Different strategies for stock maintenance or genetic drift may have left the two derivative strains with different sex determination systems. It has recently been shown that different zebrafish mating pairs can repeatedly produce widely different sex ratios, from about 5% to about 97% males, consistent with variation in sex determining genes .
An alternative explanation for finding different sex associated loci in different experiments is that the environmental conditions under which the ABxIN and *ABxNA crosses were grown differed in subtle, unknown ways important for sex determination and it could be that different sex determining genes become more important in different environments. Recent experiments ,  show that high temperature alters sex ratios in zebrafish in a genotype-specific fashion.
Precedents exist for strain-specific sex determination mechanisms in other fish. Most guppy families (Poecilia reticulata) have an XX/XY mechanism, but autosomal factors in some families can cause XY- or XX-sex reversal , ; the platyfish (X. maculatus) has three female genotypes (XX, XW, or ZW) and two male genotypes (YY or XY) ; and the red top zebra cichlid (M. pyrsonotus) can have XY and ZW sex determination systems that both segregate even within the same family . Furthermore, closely related species of stickleback, medaka, salmonids, and cichlids all appear to have evolved sex chromosomes involving different linkage groups –.
Four sex-associated regions have now been identified in zebrafish. Three of these regions (sar3, sar5, sar16) contain genes known to influence sex development, genes we might a priori consider to be candidate genes for sex determination (Figure 6, Table S7). Bradley et al.  considered two candidate genes, dmrt1 (sar5), and cyp21a2 (sar16) in their study. They identified compelling, sex-specific mutations in these genes that could suggest mechanisms for sex determination in zebrafish; to our knowledge, however, no reports yet confirm the causative roles of these genes and polymorphisms in sex determination in zebrafish. Interestingly, the putative functional SNPs in dmrt1 are not associated with sex in our stocks of AB wild-type fish; this result could be due to strain-specific differences in sex determination in these fish. Regardless, additional strong candidate genes reside within sar5 and sar16 that could underlie sex determination in the ABxIN cross, including fancg, which lies closer to the peak of sar5 than dmrt1 (Figure 6, Table S7). Zebrafish that would otherwise have become females develop as sex-reversed males when homozygous for mutations in Fanconi anemia genes (fanc genes) , , which are essential to repair DNA damage by homologous recombination . fancg and other genes already known to function in sex development, should be explored along with dmrt1 and cyp21a2 in efforts to identify the genetic elements underlying the associations between in sar5 and sar16 and sex.
The sex-associated loci we identified, sar3 and sar4, suggest important avenues for future functional investigations. The 1.5 LOD drop interval of sar3 contains about 315 annotated protein-coding genes in Zv9. Within this interval lies hsd17b1, which encodes the enzyme hydroxysteroid (17-â) dehydrogenase-1 (Figure 6). Hsd17b1 converts low-activity estrone to high-activity 17â-estradiol in zebrafish and in mammalian ovarian granulosa cells , and thus, along with Cyp19a1 (aromatase) catalyzes the final steps in estradiol biosynthesis from theca cell-derived androgens , . In zebrafish adult ovaries, hsd17b1 is expressed in Stage I follicles and peaks at Stage II, in parallel with the expression of cyp19a1a aromatase , , . The expression of hsd17b1 has not yet been investigated during the times at which sex determination occurs in zebrafish. Our model for zebrafish sex determination is that a signal from meiotic oocytes developing in gonads of the juvenile hermaphrodite stage of all individuals causes the soma to maintain the production of estrogen, which preserves ovary development and female differentiation; this suggests the hypothesis that differential regulation of hsd17b1 is important in zebrafish sex determination. In humans, mutations altering the Hsd17b3 enzyme, which performs a similar reaction in the production of testosterone and is expressed in the mammalian testis and in vitellogenic ovarian follicles in zebrafish, cause reduced masculinization in childhood but virilization in adulthood; women (lacking testes) are asymptomatic .
The association between sex and the end of chr-4 (sar4) was observed in both Family A and Family B. Clearly, the identification of the molecular genetic basis of sar4 is an important research goal, as is the discovery of the way in which it works to influence gonadal development. Unfortunately, an examination of the annotated genes in sar4 does not provide an obvious strong candidate for a major sex determination gene (Figure 6 and Table S7). Within the 1.5 LOD drop region around sar4 lie four 5S rRNA genes, three other ncRNA genes, and 56 annotated protein-coding elements. Most of the annotated protein-coding genes are members of large families, especially zinc finger domain families. Only nine protein-coding genes in sar4 are annotated in Ensembl as orthologs of specific human genes: annotated as PSMB10, PTCHD3 (2 of 3), gbp4, ttll1, pacsin2, arfgap3, IKBIP, CKAP4, terfa, and CCT2 (1 of 2). In mouse, Ptchd3 is expressed specifically in meiotic spermatocytes , but the others, as far as is known, are not specifically expressed in gonads.
Is chr-4 a Sex Chromosome?
Sex chromosomes represent a pair of originally homologous chromosomes that diverged during evolution due to the origin of a sex determining locus on one member of the pair , –. Sex chromosomes diverge by restricting recombination, initially in the region containing the sex determining locus (e.g., in medaka ), and then gradually spreading along the chromosome; eventually, the chromosome accumulates transposable elements and pseudogenes –.
We identified a sex-associated locus on zebrafish chr-4, a chromosome that shares characteristics with sex chromosomes in other species. A key feature of chr-4 that is shared with known sex determining chromosomal regions is suppressed recombination –. We found that recombination near sar4 is substantially reduced in males relative to females (Figure 4). Because recombination between sex chromosomes is usually repressed in the heterogametic sex, this result might indicate that males are the heterogametic sex in zebrafish. Interestingly however, it has been proposed that sex determination in zebrafish is controlled by female-dominant genetic factors, as if zebrafish had a ZW/ZZ not an XX/XY sex-determining system . The polygenic nature of sex determination in zebrafish (herein and ), might explain this apparent conflict. While chr-4 may be a sex chromosome, sar4 is not the only sex-associated locus in zebrafish. Genes at sar3, sar4, sar5, and sar16 may differ in whether they are female-dominant or male-dominant as is seen in sex-determining genes in cichlids . Additional work must be done to determine how each of the 4 known zebrafish sex-associated regions influence sex and how they interact.
Chr-4 is also similar to known sex chromosomes in that it is late replicating, highly heterochromatic, and repeat rich (Figure 5, Table 2). Chr-4q contains a heterochromatic region that is approximately 34 Mb long. This region is enriched for genetic elements, including 5S RNA motifs, miRNA repeats, a number of repetitive gene families, snRNA genes, tRNA genes, the transposable element Kolobok-1, and pseudogenes. Sex chromosomes often accumulate transposable elements –, . Interestingly, chr-4 is not enriched for transposable elements per se, (see Figure S2), but contains multiple copies of Kolobok 1, a member of the autonomous Kolobok transposon family . Chr-4 is also unique in having more than twice the average percentage of its chromosome length associated with copy number variant elements .
One interesting repetitive element on chr-4 is the cluster of 55 members of the mir-430 gene family at about 28.0 Mb. Similar mir-430 clusters are found in other teleost genomes, e.g. on medaka chr-4 and stickleback chromosome-IV. Mir-430 helps to limit Nanos1 and TDRD7 proteins to primordial germ cells (PGCs) in zebrafish  and similar results have been found for medaka . Mir-430 also regulates the production of Sdf1a, a ligand secreted by somatic tissues that specifies the path along which primary germ cells (PGCs) migrate to the site of the future gonad during gastrulation . Inhibiting the regulation of sdf1a leads to the mis-localization of germ cells, with fewer germ cells migrating to the gonad. In several species of fish, germ cell number appears to be an important variable distinguishing the development of an ovary (more PGCs) from a testis (fewer PGCs), and the strength of the PGC homing process could control the number of germ cells in the gonad –.
According to our genomic analysis, chr-4q appears to be a mature sex chromosome, despite the fact that sar4 is not as strong of a sex determinant as is Sry in mammals or dmrt1by in medaka. Thus, we speculate that chr-4q is an aging sex chromosome and that zebrafish is evolving new loci, like sar3, sar5, or sar16 that may be variously emerging as new, young sex determinants. It is urgent to examine species closely related to the zebrafish Danio rerio to test this hypothesis.
Our results reveal that zebrafish has a polygenic sex determining system with an important determinant occupying a short 2 Mb region at the tip of chr-4q, the only arm in the zebrafish genome that shares many features of sex chromosomes. Although several loci have been identified that affect zebrafish sex determination, as yet, no specific genes have been shown to be responsible for the sex-associated effects at any of the loci. Identification of these genes represents an important research agenda.
Genetic maps for Family A and Family B. Each vertical column represents one of the 25 zebrafish chromosomes. Each horizontal bar represents the location of a RAD-tag marker on the genetic map. Red rectangles approximate the location of sex-associated regions.
The distribution of repetitive elements and Kolobok transposons in the zebrafish genome and on chr-4 specifically. A. Distribution of all repeats and Kolobok elements across the entire genome. B. Distribution of all repeats and Kolobok elements across chr-4. C. Ideogram showing the location of the miRNA-430 gene cluster, the heterochromatic region of chr-4, and sar4. D. Number of Kolobok elements in the genome (# genome), in the heterochromatic region of chr-4 (# het region Dre4), and % of each Kolobok element in the heterochromatic region of chr-4 (% het region).
Characteristics of polymorphic RAD-tag markers used for genetic mapping in Family A.
Characteristics of polymorphic RAD-tag markers used for genetic mapping in Family B.
Counts of female and male F2 offspring in each haplotype category for sar3 (Table S4, Family B), and sar4 (Tables S3 and S4, Family A and Family B). These data were used to calculate the percent male values presented in Figure 2 A, B.
Counts of female and male F2 offspring in each haplotype category for sar3 (Table S4, Family B), and sar4 (Tables S3 and S4, Family A and Family B). These data were used to calculate the percent male values presented in Figure 2 A, B.
Details for SNPs found at significantly different frequencies in females and males including chromosome, position in the Zv9 genome assembly, G-test statistic and associated p-value, observed alleles (alleles 1 and 2), the numbers of females and males of each genotype, and the total numbers of individuals scored.
Details for SNPs found at significantly different frequencies in females and males including chromosome, position in the Zv9 genome assembly, G-test statistic and associated p-value, observed alleles (alleles 1 and 2), the numbers of females and males of each genotype, and the total numbers of individuals scored.
Genomic locations of candidate genes for sex determination in the zebrafish genome (genome assembly Zv9, Ensembl version 65).
A Fixer.py custom script that recodes genotypes from JoinMap family type CP for analysis as a phase-known 4-way cross in R/qtl.
Partial sequence of dmrt1 from Danio rerio. Heterozygous positions noted using IUPAC ambiguity code. Orange boxes indicate sequence associated with ss184964113 and ss184964140 in the dbSNP database at NCBI (http://www.ncbi.nlm.nih.gov/projects/SNP/), the SNPs identified as potentially functional by Bradley et al. (2011).
We are grateful to Joy Murphy, Amber Starks, Amanda Rapp, Catherine Wilson, Marcy McFadden, Rebecca Montgomery, Tim Mason, and the University of Oregon Zebrafish Facility for providing animals and excellent fish care. We thank Cristian Cañestro and Levi Simonson for help with DNA analyses; Nick Stiffler and Doug Turnbull for help with Illumina sequencing, Amanda Kenney for help in recoding data for R/qtl, and Cristian Cañestro, William Cresko, Susan Bassham and Julian Catchen for thoughtful suggestions and discussions.
Conceived and designed the experiments: JLA ARM IB JHP. Performed the experiments: JLA ARM AA IB PH PB. Analyzed the data: JLA AA IB PH JHP. Contributed reagents/materials/analysis tools: PH PB. Wrote the paper: JLA JHP.
- 1. Sinclair AH, Berta P, Palmer MS, Hawkins JR, Griffiths BL, et al. (1990) A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif. Nature 346: 240–244.
- 2. Ser JR, Roberts RB, Kocher TD (2010) Multiple interacting loci control sex determination in Lake Malawi cichlid fish. Evolution 64: 486–501.
- 3. Bull JJ, Vogt RC (1979) Temperature-dependent sex determination in turtles. Science 206: 1186–1188.
- 4. Ferguson MW, Joanen T (1982) Temperature of egg incubation determines sex in Alligator mississippiensis. Nature 296: 850–853.
- 5. Crews D, Wibbels T, Gutzke WH (1989) Action of sex steroid hormones on temperature-induced sex determination in the snapping turtle (Chelydra serpentina). Gen Comp Endocrinol 76: 159–166.
- 6. Kim Y, Capel B (2006) Balancing the bipotential gonad between alternative organ fates: a new perspective on an old problem. Dev Dyn 235: 2292–2300.
- 7. Pieau C, Dorizzi M, Richard-Mercier N (1999) Temperature-dependent sex determination and gonadal differentiation in reptiles. Cell Mol Life Sci 55: 887–900.
- 8. Baroiller JF, Guiguen Y (2001) Endocrine and environmental aspects of sex differentiation in gonochoristic fish. EXS. pp. 177–201.
- 9. Nanda I, Kondo M, Hornung U, Asakawa S, Winkler C, et al. (2002) A duplicated copy of DMRT1 in the sex-determining region of the Y chromosome of the medaka, Oryzias latipes. Proc Natl Acad Sci U S A 99: 11778–11783.
- 10. Matsuda M, Nagahama Y, Shinomiya A, Sato T, Matsuda C, et al. (2002) DMY is a Y-specific DM-domain gene required for male development in the medaka fish. Nature 417: 559–563.
- 11. Crews D (2003) Sex determination: where environment and genetics meet. Evol Dev 5: 50–55.
- 12. Sato T, Endo T, Yamahira K, Hamaguchi S, Sakaizumi M (2005) Induction of female-to-male sex reversal by high temperature treatment in medaka, Oryzias latipes. Zoolog Sci 22: 985–988.
- 13. Barske LA, Capel B (2008) Blurring the edges in vertebrate sex determination. Curr Opin Genet Dev 18: 499–505.
- 14. Pijnacker LP, Ferwerda MA (1995) Zebrafish chromosome banding. Genome 38: 1052–1055.
- 15. Daga RR, Thode G, Amores A (1996) Chromosome complement, C-banding, Ag-NOR and replication banding in the zebrafish Danio rerio. Chromosome Res 4: 29–32.
- 16. Amores A, Postlethwait JH (1999) Banded chromosomes and the zebrafish karyotype. Methods Cell Biol 60: 323–338.
- 17. Traut W, Winking H (2001) Meiotic chromosomes and stages of sex chromosome evolution in fish: zebrafish, platyfish and guppy. Chromosome Res 9: 659–672.
- 18. Phillips RB, Amores A, Morasch MR, Wilson C, Postlethwait JH (2006) Assignment of zebrafish genetic linkage groups to chromosomes. Cytogenet Genome Res 114: 155–162.
- 19. Tong SK, Hsu HJ, Chung BC (2010) Zebrafish monosex population reveals female dominance in sex determination and earliest events of gonad differentiation. Dev Biol 344: 849–856.
- 20. Uchida D, Yamashita M, Kitano T, Iguchi T (2002) Oocyte apoptosis during the transition from ovary-like tissue to testes during sex differentiation of juvenile zebrafish. J Exp Biol 205: 711–718.
- 21. Pelegri F, Schulte-Merker S (1999) A gynogenesis-based screen for maternal-effect genes in the zebrafish, Danio rerio. Methods Cell Biol 60: 1–20.
- 22. Liew WC, Bartfai R, Lim Z, Sreenivasan R, Siegfried KR, et al. (2012) Polygenic sex determination system in zebrafish. PLoS ONE 7: e34397.
- 23. Bradley KM, Breyer JP, Melville DB, Broman KW, Knapik EW, et al. (2011) An SNP-based linkage map for zebrafish reveals sex determination loci. G3 1: 3–9.
- 24. Chan STH, Yeung WSB (1983) Sex control and sex reversal in fish under natural conditions. In: Hoar WS, Randall DJ, Donaldson EM, editors. pp. 171–222. New York: Academic Press.
- 25. Uchida D, Yamashita M, Kitano T, Iguchi T (2004) An aromatase inhibitor or high water temperature induce oocyte apoptosis and depletion of P450 aromatase activity in the gonads of genetic female zebrafish during sex-reversal. Comp Biochem Physiol A Mol Integr Physiol 137: 11–20.
- 26. Shang EH, Yu RM, Wu RS (2006) Hypoxia affects sex differentiation and development, leading to a male-dominated population in zebrafish (Danio rerio). Environ Sci Technol 40: 3118–3122.
- 27. Abozaid H, Wessels S, Horstgen-Schwark G (2011) Effect of rearing temperatures during embryonic development on the phenotypic sex in zebrafish (Danio rerio). Sex Dev 5: 259–265.
- 28. Siegfried KR (2010) In search of determinants: gene expression during gonadal sex differentiation. J Fish Biol 76: 1879–1902.
- 29. Rodríguez-Marí A, Postlethwait JH (2011) The role of Fanconi anemia/BRCA genes in zebrafish sex determination. Methods Cell Biol 105: 461–490.
- 30. Maack G, Segner H (2003) Morphological development of the gonads in zebrafish. J Fish Biol 62: 895–906.
- 31. Wang XG, Orban L (2007) Anti-Müllerian hormone and 11 β-hydroxylase show reciprocal expression to that of aromatase in the transforming gonad of zebrafish males. Dev Dyn 236: 1329–1338.
- 32. Rodríguez-Marí A, Cañestro C, BreMiller RA, Nguyen-Johnson A, Asakawa K, et al. (2010) Sex reversal in zebrafish fancl mutants results from Tp53-mediated germ cell apoptosis. PLoS Genet 6: e1001034.
- 33. Rodríguez-Marí A, Wilson C, Titus TA, Cañestro C, Bremiller RA, et al. (2011) Roles of brca2 (fancd1) in oocyte nuclear architecture, gametogenesis, gonad tumors, and genome stability in zebrafish. PLoS Genet 7: e1001357.
- 34. Siegfried KR, Nusslein-Volhard C (2008) Germ line control of female sex determination in zebrafish. Dev Biol 324: 277–287.
- 35. Wang X, Bartfai R, Sleptsova-Freidrich I, Orban L (2007) The timing and extent of ‘juvenile ovary’ phase are highly variable during zebrafish testis differentiation. J Fish Biol 70: 33–44.
- 36. Slanchev K, Stebler J, de la Cueva-Mendez G, Raz E (2005) Development without germ cells: the role of the germ line in zebrafish sex differentiation. Proc Natl Acad Sci U S A 102: 4074–4079.
- 37. Draper BW, McCallum CM, Moens CB (2007) nanos1 is required to maintain oocyte production in adult zebrafish. Dev Biol 305: 589–598.
- 38. Houwing S, Kamminga LM, Berezikov E, Cronembold D, Girard A, et al. (2007) A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in zebrafish. Cell 129: 69–82.
- 39. Houwing S, Berezikov E, Ketting RF (2008) Zili is required for germ cell differentiation and meiosis in zebrafish. Embo J 27: 2702–2711.
- 40. Shive HR, West RR, Embree LJ, Azuma M, Sood R, et al. (2010) brca2 in zebrafish ovarian development, spermatogenesis, and tumorigenesis. Proc Natl Acad Sci U S A 107: 19350–19355.
- 41. Kamminga LM, Luteijn MJ, den Broeder MJ, Redl S, Kaaij LJ, et al. (2010) Hen1 is required for oocyte development and piRNA stability in zebrafish. EMBO J 29: 3688–3700.
- 42. Skakkebaek NE, Jørgensen N, Main KM, Rajpert-De Meyts E, Leffers H, et al. (2006) Is human fecundity declining? Int J Androl 29: 2–11.
- 43. Dumesic DA, Abbott DH, Padmanabhan V (2007) Polycystic ovary syndrome and its developmental origins. Rev Endocr Metab Disord 8: 127–141.
- 44. Cnaani A, Lee BY, Zilberman N, Ozouf-Coastaz C, Hulata G, et al. (2008) Genetics of sex determination in tilapiine species. Sex Dev 2: 43–54.
- 45. Martınez P, Bouza C, Hermida M, Fernandez J, Toro A, et al. (2009) Identification of the major sex-determining region of turbot (Scophthalmus maximus). Genetics 183: 1443–1452.
- 46. Loukovitis D, Sarropoulou E, Tsigenopoulos CS, Batargias C, Magoulas A, et al. (2011) Quantitative trait loci involved in sex determination and body growth in the gilthead sea bream (Sparus aurata L.) through targeted genome scan. PLoS One 6: e16599.
- 47. Smith CA, Roeszler KN, Ohnesorg T, Cummins DM, Farlie PG, et al. (2009) The avian Z-linked gene DMRT1 is required for male sex determination in the chicken. Nature 461: 267–271.
- 48. Yoshimoto S, Ito M (2011) A ZZ/ZW-type sex determination in Xenopus laevis. FEBS J 278: 1020–1026.
- 49. Baumgartner-Parzer SM, Schulze E, Waldhäusl W, Pauschenwein S, Rondot S, et al. (2001) Mutational spectrum of the steroid 21-hydroxylase gene in Austria: identification of a novel missense mutation. J Clin Endocrinol Metab 86: 4771–4775.
- 50. Schultheis C, Bohne A, Schartl M, Volff JN, Galiana-Arnoux D (2009) Sex determination diversity and sex chromosome evolution in poeciliid fish. Sex Dev 3: 68–77.
- 51. Baird NA, Etter PD, Atwood TS, Currey MC, Shiver AL, et al. (2008) Rapid SNP discovery and genetic mapping using sequenced RAD markers. PLoS ONE 3: e3376.
- 52. Amores A, Catchen JM, Ferrara A, Fontenot Q, Postlethwait JH (2011) Genome evolution and meiotic maps by massively parallel DNA sequencing: spotted gar, an outgroup for the teleost genome duplication. Genetics 188: 1–10.
- 53. Hohenlohe PA, Bassham S, Etter PD, Stiffler N, Johnson EA, et al. (2011) Population genomics of parallel adaptation in threespine stickleback using sequenced RAD tags. PLoS Genet 6: e1000862.
- 54. Streisinger G, Walker C, Dower N, Knauber D, Singer F (1981) Production of clones of homozygous diploid zebra fish (Brachydanio rerio). Nature 291: 293–296.
- 55. Westerfield M (1995) The zebrafish book: a guide for the laboratory use of zebrafish (Danio rerio). Eugene, OR: University of Oregon Press.
- 56. Catchen JM, Amores A, Hohenlohe PA, Cresko WA, Postlethwait JH (2011) Stacks: de novo genotype analyses from short-read sequence data. G3 1: 1–10.
- 57. Langmead B, Trapnell C, Pop M, Salzberg SL (2009) Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10: R25.
- 58. Van Ooijen JW (2006) JoinMap 4, Software for the calculation of genetic linkage maps in experimental populations. Wageningen, Netherlands: Kyazma B. V p.
- 59. Broman KW, Wu H, Sen S, Churchill GA (2003) R/qtl: QTL mapping in experimental crosses. Bioinformatics 19: 889–890.
- 60. Postlethwait JH, Johnson S, Midson CN, Talbot WS, Gates M, et al. (1994) A genetic linkage map for the zebrafish. Science 264: 699–703.
- 61. Johnson SL, Gates MA, Johnson M, Talbot WS, Horne S, et al. (1996) Centromere-linkage analysis and consolidation of the zebrafish genetic map. Genetics 142: 1277–1288.
- 62. Gornung E, Gabrielli I, Cataudella S, Sola L (1997) CMA3-banding pattern and fluorescence in situ hybridization with 18S rRNA genes in zebrafish chromosomes. Chromosome Res 5: 40–46.
- 63. Gornung E, De Innocentiis S, Annesi F, Sola L (2000) Zebrafish 5S rRNA genes map to the long arms of chromosome 3. Chromosome Res 8: 362.
- 64. Sola L, Gornung E (2001) Classical and molecular cytogenetics of the zebrafish, Danio rerio (Cyprinidae, Cypriniformes): an overview. Genetica 111: 397–412.
- 65. Goudet J, Raymond M, de Meeus T, Rousset F (1996) Testing differentiation in diploid populations. Genetics 144: 1933–1940.
- 66. Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. J Roy Stat Soc B 57: 289–300.
- 67. Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, et al. (2011) Geneious v5.4. http://www.geneious.com/.
- 68. Robinson JT, Thorvaldsdottir H, Winckler W, Guttman M, Lander ES, et al. (2011) Integrative genomics viewer. Nat Biotechnol 29: 24–26.
- 69. Alvarez MC, Otis J, Amores A, Guise K (1991) Short-term cell culture technique for obtaining chromosomes in marine and freshwater fish. J Fish Biol 39: 817–824.
- 70. Freeman JL, Adeniy A, Banerjee R, Dallaire S, Maguire SF, et al. (2007) Definition of the zebrafish genome using flow cytometry and cytogenetic mapping. BMC Genomics 8: 195.
- 71. Guo Y, Cheng H, Huang X, Gao S, Yu H, et al. (2005) Gene structure, multiple alternative splicing, and expression in gonads of zebrafish Dmrt1. Biochem Biophys Res Commun 330: 950–957.
- 72. Herpin A, Nakamura S, Wagner TU, Tanaka M, Schartl M (2009) A highly conserved cis-regulatory motif directs differential gonadal synexpression of Dmrt1 transcripts during gonad development. Nucleic Acids Res 37: 1510–1520.
- 73. Kondo M, Hornung U, Nanda I, Imai S, Sasaki T, et al. (2006) Genomic organization of the sex-determining and adjacent regions of the sex chromosomes of medaka. Genome Res 16: 815–826.
- 74. Peichel CL, Ross JA, Matson CK, Dickson M, Grimwood J, et al. (2004) The master sex-determination locus in threespine sticklebacks is on a nascent Y chromosome. Curr Biol 14: 1416–1424.
- 75. Charlesworth D, Charlesworth B, Marais G (2005) Steps in the evolution of heteromorphic sex chromosomes. Heredity 95: 118–128.
- 76. Bergero R, Charlesworth D (2009) The evolution of restricted recombination in sex chromosomes. Trends Ecol Evol 24: 94–102.
- 77. Singer A, Perlman H, Yan Y, Walker C, Corley-Smith G, et al. (2002) Sex-specific recombination rates in zebrafish (Danio rerio). Genetics 160: 649–657.
- 78. Johnson SL, Africa D, Horne S, Postlethwait JH (1995) Half-tetrad analysis in zebrafish: mapping the ros mutation and the centromere of linkage group I. Genetics 139: 1727–1735.
- 79. Kauffman EJ, Gestl EE, Kim DJ, Walker C, Hite JM, et al. (1995) Microsatellite-centromere mapping in the zebrafish (Danio rerio). Genomics 30: 337–341.
- 80. Mohideen MA, Moore JL, Cheng KC (2000) Centromere-linked microsatellite markers for linkage groups 3, 4, 6, 7, 13, and 20 of zebrafish (Danio rerio). Genomics 67: 102–106.
- 81. Yuan YW, Wessler SR (2011) The catalytic domain of all eukaryotic cut-and-paste transposase superfamilies. Proc Natl Acad Sci U S A 108: 7884–7889.
- 82. Grumbach MM, Morishima A, Taylor JH (1963) Human sex chromosome abnormalities in relation to DNA replication and heterochromatinization. Proc Natl Acad Sci U S A 49: 581–589.
- 83. Kallman KD (1984) A new look at sex determination in poeciliid fishes. In: Turner BJ, editor. pp. 95–171. New York: Plenum Publishing.
- 84. Brown KH, Dobrinski KP, Lee AS, Gokcumen O, Mills RE, et al. (2012) Extensive genetic diversity and substructuring among zebrafish strains revealed through copy number variant analysis. Proc Natl Acad Sci U S A 109: 529–534.
- 85. Engeszer RE, Patterson LB, Rao AA, Parichy DM (2007) Zebrafish in the wild: a review of natural history and new notes from the field. Zebrafish 4: 21–40.
- 86. Whiteley AR, Bhat A, Martins EP, Mayden RL, Arunachalam M, et al. (2011) Population genomics of wild and laboratory zebrafish (Danio rerio). Mol Ecol.
- 87. Abozaid H, Wessels S, Horstgen-Schwark G (2012) Elevated temperature applied during gonadal transformation leads to male bias in zebrafish (Danio rerio). Sex Dev [Epub ahead of print]. DOI:10.1159/000336297.
- 88. Winge O (1930) On the occurrence of XX males in Lebistes with some remarks on Aida’s so called ‘non-disjunctional males’ in Aplocheilus. J Genet 23: 69–76.
- 89. Nayudu PL (1979) Genetic studies of melanic color patterns, and atypical sex determination in the guppy, Poecilia reticulata. Copeia 2: 225–231.
- 90. Kondo M, Nanda I, Hornung U, Schmid M, Schartl M (2004) Evolutionary origin of the medaka Y chromosome. Curr Biol 14: 1664–1669.
- 91. Roberts RB, Ser JR, Kocher TD (2009) Sexual conflict resolved by invasion of a novel sex determiner in Lake Malawi cichlid fishes. Science 326: 998–1001.
- 92. Ross JA, Urton JR, Boland J, Shapiro MD, Peichel CL (2009) Turnover of sex chromosomes in the stickleback fishes (gasterosteidae). PLoS Genet 5: e1000391.
- 93. Davidson WS, Huang TK, Fujiki K, von Schalburg KR, Koop BF (2009) The sex determining loci and sex chromosomes in the family salmonidae. Sex Dev 3: 78–87.
- 94. Kee Y, D’Andrea AD (2010) Expanded roles of the Fanconi anemia pathway in preserving genomic stability. Genes Dev 24: 1680–1694.
- 95. Mindnich R, Deluca D, Adamski J (2004) Identification and characterization of 17β-hydroxysteroid dehydrogenases in the zebrafish, Danio rerio. Mol Cell Endocrinol 215: 19–30.
- 96. Sawetawan C, Milewich L, Word RA, Carr BR, Rainey WE (1994) Compartmentalization of type I 17 β-hydroxysteroid oxidoreductase in the human ovary. Mol Cell Endocrinol 99: 161–168.
- 97. Poutanen M, Isomaa V, Peltoketo H, Vihko R (1995) Role of 17β-hydroxysteroid dehydrogenase type 1 in endocrine and intracrine estradiol biosynthesis. J Steroid Biochem Mol Biol 55: 525–532.
- 98. Rodríguez-Marí A, Yan YL, Bremiller RA, Wilson C, Cañestro C, et al. (2005) Characterization and expression pattern of zebrafish anti-Müllerian hormone (amh) relative to sox9a, sox9b, and cyp19a1a, during gonad development. Gene Expr Patterns 5: 655–667.
- 99. Ings JS, Van Der Kraak GJ (2006) Characterization of the mRNA expression of StAR and steroidogenic enzymes in zebrafish ovarian follicles. Mol Reprod Dev 73: 943–954.
- 100. Rosler A, Silverstein S, Abeliovich D (1996) A (R80Q) mutation in 17β-hydroxysteroid dehydrogenase type 3 gene among Arabs of Israel is associated with pseudohermaphroditism in males and normal asymptomatic females. J Clin Endocrinol Metab 81: 1827–1831.
- 101. Fan J, Akabane H, Zheng X, Zhou X, Zhang L, et al. (2007) Male germ cell-specific expression of a novel Patched-domain containing gene Ptchd3. Biochem Biophys Res Commun 363: 757–761.
- 102. Skaletsky H, Kuroda-Kawaguchi T, Minx PJ, Cordum HS, Hillier L, et al. (2003) The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes. Nature 423: 825–837.
- 103. Matsubara K, Tarui H, Toriba M, Yamada K, Nishida-Umehara C, et al. (2006) Evidence for different origin of sex chromosomes in snakes, birds, and mammals and step-wise differentiation of snake sex chromosomes. Proc Natl Acad Sci U S A 103: 18190–18195.
- 104. Marshall Graves JA (2008) Weird animal genomes and the evolution of vertebrate sex and sex chromosomes. Annu Rev Genet 42: 565–586.
- 105. Tripathi N, Hoffmann M, Weigel D, Dreyer C (2009) Linkage analysis reveals the independent origin of Poeciliid sex chromosomes and a case of atypical sex inheritance in the guppy (Poecilia reticulata). Genetics 182: 365–374.
- 106. Nanda I, Volff JN, Weis S, Korting C, Froschauer A, et al. (2000) Amplification of a long terminal repeat-like element on the Y chromosome of the platyfish, Xiphophorus maculatus. Chromosoma 109: 173–180.
- 107. Mishima Y, Giraldez AJ, Takeda Y, Fujiwara T, Sakamoto H, et al. (2006) Differential regulation of germline mRNAs in soma and germ cells by zebrafish miR-430. Curr Biol 16: 2135–2142.
- 108. Tani S, Kusakabe R, Naruse K, Sakamoto H, Inoue K (2010) Genomic organization and embryonic expression of miR-430 in medaka (Oryzias latipes): insights into the post-transcriptional gene regulation in early development. Gene 449: 41–49.
- 109. Staton AA, Knaut H, Giraldez AJ (2011) miRNA regulation of Sdf1 chemokine signaling provides genetic robustness to germ cell migration. Nat Genet 43: 204–211.
- 110. Lewis ZR, McClellan MC, Postlethwait JH, Cresko WA, Kaplan RH (2008) Female-specific increase in primordial germ cells marks sex differentiation in threespine stickleback (Gasterosteus aculeatus). J Morphol 269: 909–921.
- 111. Quirk JG, Hamilton JB (1973) Number of germ cells in known male and known female genotypes of vertebrate embryos (Oryzias latipes). Science 180: 963–964.
- 112. Kobayashi T, Matsuda M, Kajiura-Kobayashi H, Suzuki A, Saito N, et al. (2004) Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes. Dev Dyn 231: 518–526.