Recent studies have implicated the human cytomegalovirus (HCMV) as a possible pathogen for causing hypertension. We aimed to study the association between HCMV infection and hypertension in the United States National Health and Nutrition Examination Survey (NHANES).
We analyzed data on 2979 men and 3324 women in the NHANES 1999–2002. We included participants aged 16–49 years who had valid data on HCMV infection and hypertension.
Of the participants, 54.7% had serologic evidence of HCMV infection and 17.5% had hypertension. There were ethnic differences in the prevalence of HCMV infection (P<0.001) and hypertension (P<0.001). The prevalence of both increased with age (P<0.001). Before adjustment, HCMV seropositivity was significantly associated with hypertension in women (OR = 1.63, 95% CI = 1.25–2.13, P = 0.001) but not in men. After adjustment for race/ethnicity, the association between HCMV seropositivity and hypertension in women remained significant (OR = 1.55, 95% CI = 1.20–2.02, P = 0.002). Further adjustment for body mass index, diabetes status and hypercholesterolemia attenuated the association (OR = 1.44, 95% CI = 1.10–1.90, P = 0.010). However, after adjusting for age, the association was no longer significant (OR = 1.24, 95% CI = 0.91–1.67, P = 0.162).
Citation: Li C, Samaranayake NR, Ong KL, Wong HK, Cheung BMY (2012) Is Human Cytomegalovirus Infection Associated with Hypertension? The United States National Health and Nutrition Examination Survey 1999–2002. PLoS ONE 7(7): e39760. https://doi.org/10.1371/journal.pone.0039760
Editor: Weili Zhang, FuWai hospital, Chinese Academy of Medical Sciences, China
Received: February 13, 2012; Accepted: May 28, 2012; Published: July 2, 2012
Copyright: © 2012 Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: BMYC received support from the Faculty Research Fund, Li Ka Shing Faculty of Medicine, University of Hong Kong. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Hypertension, a multi-factorial disease, is a global public health burden. ,  It is a predisposing risk factor for myocardial infarction, revascularization, heart failure, stroke and renal failure. Both genetic and environmental factors are involved in the development of hypertension. There are many known environmental factors leading to hypertension. Obesity, inappropriate diet, stress, lack of physical activity, tobacco smoking, alcoholic drinking and certain medications are known risk factors for hypertension.  In recent years, meta-analysis of genome wide association studies has highlighted a number of genetic variants associated with hypertension. .
Human cytomegalovirus (HCMV), a member of the beta herpes virus family, is a ubiquitous pathogen that infects only humans.  It is an opportunistic infection in 90% of the HIV carriers and a latent infection in over 50% of the general population.  Although HCMV infection is usually asymptomatic in healthy adults, it can infect organs and tissues virtually throughout the body. HCMV is a cause of congenital diseases, causes primary infection in children and young adults, and has been implicated in pneumonitis, retinitis and gastroenteritis in adults, particularly in immunosuppressed or immunocompromised patients, such as transplant recipients and patients with HIV infection. .
Recent studies have suggested that HCMV infection is associated with a significantly increased risk for all-cause and cardiovascular diseases (CVDs) related mortality.  It has been implicated in endothelial dysfunction  and different cardiovascular diseases, such as myocarditis, atherosclerosis and coronary artery disease. , ,  However the association between HCMV infection and hypertension is still unclear.  As hypertension is an important cause of CVDs, we studied the association between HCMV and hypertension in the United States (US) National Health and Nutrition Examination Survey (NHANES) 1999–2002.
For this analysis, data were extracted from NHANES 1999–2002, which was a continuous survey since 1999. The study protocol was approved by the Centers for Disease Control and Prevention Institutional Review Board. All participants gave written consent for their information to be stored in the database and used for research. They also gave written consent for their blood and urine samples to be kept for future research, such as the measurement of HCMV specific IgG. For participants aged 16–17 years, written consent was obtained both from them and their parents or guardians. Participants were examined in well-equipped mobile examination centers located around the US. The detailed measurement procedures and protocols have been described in previous publications and also on its website.  Participants were selected using a stratified, multistage probability sampling design from the civilian non-institutionalized US population. Among the 6610 participant aged ≥16 years who had valid data on HCMV infection and hypertension, 6303 participants with valid data on body mass index (BMI), blood pressure, diabetes and hypercholesterolemia were included in our study.
Variables of Interest
Race/ethnicity was based on self-reported questionnaires. Participants were classified as non-Hispanic whites, non-Hispanic blacks, Mexican Americans and others. HCMV infection status was determined by HCMV specific IgG test using commercial enzyme linked immunosorbent assay (ELISA) kits (Quest International Inc, Miami, FL) in a centralized manner. Sera with values near the ELISA cutoff (approximately 5.2% of total) were measured again with another commercial ELISA kit (bioMerieux Inc, Durham, NC) to confirm the results. An immunofluorescence assay (Bion International Inc, Park Ridge, IL), was used to give the final results if the results from the first two tests disagreed (approximately 2.7% of total). The optical density from the ELISA assay was reported as an approximate measure of antibody titer. A low and positive optical density reflects a low antibody titer and a high optical density reflects a high antibody titer. BMI was calculated as the weight in kilograms divided by the square of the height in meters. Blood pressure was measured manually by a trained operator using a mercury sphygmomanometer. Hypertension was defined as self-report of diagnosed hypertension, blood pressure ≥140/90 mm Hg, or use of anti-hypertensive medications. Diabetes was defined as a fasting glucose of ≥7.0 mmol/L (126 mg/dl), non-fasting glucose of ≥11.1 mmol/L (200 mg/dl), previous diagnosis by a doctor, or use of anti-diabetic medications. Hypercholesterolemia was defined as total serum cholesterol ≥240 mg/dL, previous diagnosis by a doctor, or taking prescribed medicine for hypercholesterolemia.
Data were analyzed using the complex sampling function of SPSS version 20.0 (IBM Corporation, Armonk, NY). The four-year mobile examination weight was used in all analyses to account for non-response bias, and the oversampling of blacks, Mexican Americans and the elderly in NHANES 1999–2002 so that the estimates were nationally representative according to the 2000 US Census population. P values were obtained using logistic or multiple regression analysis where appropriate, using the complex sampling function. Logistic and multiple regressions were used to adjust for covariates. Variables that significantly differed between participants with and without HCMV infection, or with and without hypertension, were used as covariates in the regression models. There was no significant interaction between HCMV seropositivity and race/ethnicity with hypertension in multiple regression analysis. Therefore all the racial/ethnic groups were combined for analysis.
Among the 6303 participants aged 16–49 years in NHANES 1999–2002, 3975 had HCMV infection (54.7±1.3%) and 939 had hypertension (17.5±0.8%). All the percentages were calculated after weighting to the 2000 US Census population.
Table 1 shows the characteristics of all participants according to HCMV seropositivity status. Participants with HCMV infection were older (P<0.001) and more likely to be women (P<0.001), compared to participants without HCMV infection. There were significant racial/ethnic differences between participants with and without HCMV infection (P<0.001).
Table 2 shows the characteristics of all participants according to hypertension status. Participants with hypertension were older (P<0.001) and had a greater BMI (P<0.001), compared to participants without hypertension. Also hypertensive participants were more likely to be men (P = 0.004), and have diabetes (P<0.001) as well as hypercholesterolemia (P<0.001). There were significant racial/ethnic differences between participants with and without hypertension (P<0.001).
Figure 1 shows univariate logistic regression analysis of hypertension. In this analysis, HCMV seropositivity was associated with hypertension in women (OR = 1.63, 95% CI = 1.25–2.13, P = 0.001) but the association in men was not significant (OR = 0.94, 95% CI = 0.75–1.20, P = 0.620).
Table 3 shows the logistic regression analysis after adjusting for covariates. There was no significant association between HCMV seropositivity and hypertension in men in any of the models. The association between HCMV seropositivity and hypertension in women remained significant after adjusting for race/ethnicity (OR = 1.55, 95% CI = 1.20–2.02, P = 0.002). Further adjustment for BMI, diabetes status and hypercholesterolemia attenuated the association (OR = 1.44, 95% CI = 1.10–1.90, P = 0.010). However, after adjusting for age, the association was no longer significant (OR = 1.24, 95% CI = 0.91–1.67, P = 0.162).
Table 4 shows the association between optical density for the HCMV specific IgG assay and blood pressure as a continuous variable among participants who were not taking any antihypertensive medication. In men, there was no significant relationship between optical density for the HCMV specific IgG assay and systolic or diastolic blood pressure before adjustment (P = 0.144). After adjusting for race/ethnicity, there is a significant association between optical density for the HCMV specific IgG assay and diastolic blood pressure (P = 0.033). Further adjustment for body mass index, diabetes status and hypercholesterolemia did not attenuate the association (P = 0.029). However, after adjusting for age, the association was no longer significant (P = 0.815). In contrast, optical density for the HCMV specific IgG assay was associated with both systolic and diastolic blood pressure in women, but this association was no longer significant after controlling for age. The conclusions remained the same if we included participants on antihypertensive medication but added 10/5 mmHg to their blood pressure on treatment. .
Our study used data from the large and nationally representative NHANES study to investigate the association between HCMV and hypertension. It shows that there is no strong evidence to support HCMV as a significant cause of hypertension. Although HCMV seropositivity was significantly associated with hypertension in women, the association was greatly diminished after adjusting for age. This means that the association of hypertension with HCMV seropositivity in women can largely be explained by the increased likelihood of having had HCMV infection with age. A similar trend also appeared in the association between HCMV seropositivity and diastolic blood pressure in men. A previous study involving 1074 women and 857 men (aged 24–39 years) from the Cardiovascular Risk in Young Finns study has shown that CMV antibody titers were independent determinants for systolic and diastolic blood pressure elevation and flow-mediated dilation in men, but not in women. Discrepancies between the Cardiovascular Risk in Young Finns Study and our study might be due to differences in sample size, age range, socioeconomic factors, dietary habits and climate.  However, our study has the advantages of having a larger sample size, wider age range and several racial/ethnic groups.
Although no strong evidence that HCMV is a significant cause of hypertension has been found in clinical studies, there is a theoretical basis for this association and several mechanisms can be envisaged. First, HCMV infection could lead to oxidative stress and endothelial dysfunction. , , , , ,  As endothelial dysfunction and inflammation are two key mechanisms in the development and progression of hypertension, ,  this provides an explanation of how chronic HCMV infection may result in hypertension. Second, HCMV infection triggers the proliferation of one type of T lymphocytes, CD8+ cytotoxic T lymphocytes. ,  The clonal expansion of HCMV-specific T lymphocytes may impair the response to other antigens. It is also known that T lymphocytes take part in the alteration of vascular tone encountered hypertension induced by angiotensin II, which is a potent vasoconstrictor.  Therefore, T cells may be a link between HCMV infection and hypertension. Third, recent research has suggested that microRNAs (miRNAs), a class of post-transcriptional gene expression regulators, are present in the circulation and may serve as potential biomarkers.  From a genetic point of view, circulating miRNAs carry disease-specific information owing to inherent genomic alterations. A recent study showed HCMV infection was significantly correlated with the miRNA (hcmv-miRUL112) which is encoded by HCMV and highly expressed in hypertensive patients compared to healthy control subjects.  This suggested a possible link between HCMV infection and hypertension at the genomic level as well. Fourth, mouse cytomegalovirus (MCMV), the animal counterpart of HCMV, has been demonstrated to activate over-expression of angiotensin II in the renin-angiotensin-aldosterone system (RAAS), which plays an important part in the emergence of hypertension.  Angiotensin II causes vasoconstriction, and also stimulates generation and secretion of ROS, inflammatory cytokines, chemokines and adhesion molecules.  Therefore it is possible that HCMV has similar effects on the human RAAS, so further research to elucidate the role of HCMV in hypertension is still needed.
This study has provided some important insights into HCMV and hypertension. The results derived from a large nationally-representative population are reliable due to good sampling design and quality control. It shows that age may account for a large part of the association of HCMV infection with hypertension. Some risk factors known to be closely related to hypertension, such as alcohol consumption, smoking and physical activity, contribute to the development and progression of hypertension. In this study, we did not include these factors as covariates in the adjustment of the association of HCMV with hypertension because there were a substantial number of subjects with missing values in these factors in the NHANES database. Incorporating these risk factors in our model would have reduced the sample size and thereby the power of our analysis. Further studies with larger sample sizes and more comprehensive information on risk factors are needed to elucidate the complex relationship between HCMV and hypertension.
In conclusion, HCMV seropositivity is associated with hypertension in women in the NHANES population. This association is largely explained by the association of hypertension with age and the increase in past exposure to HCMV with age.
Conceived and designed the experiments: BMYC. Analyzed the data: CL. Wrote the paper: CL NRS KLO HKW BMYC. Gave advice on the analysis: BMYC KLO NRS.
- 1. Cheung BMY, Ong KL (2011) The Challenge of Managing Hypertension. In: Finkel ML, Westport CT, editors. pp. 117–128.BMY CheungKL Ong2011The Challenge of Managing Hypertension.ML FinkelCT Westport117128editors.Public health in the 21st century.Praeger Press. Praeger Press.
- 2. Ong KL, Cheung BM, Man YB, Lau CP, Lam KS (2007) Prevalence, awareness, treatment, and control of hypertension among United States adults 1999–2004. Hypertension 49: 69–75.KL OngBM CheungYB ManCP LauKS Lam2007Prevalence, awareness, treatment, and control of hypertension among United States adults 1999–2004.Hypertension496975
- 3. Chaturvedi S (2004) The Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure (JNC 7): is it really practical? Natl Med J India 17: 227.S. Chaturvedi2004The Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure (JNC 7): is it really practical?Natl Med J India17227
- 4. Taal HR, Verwoert GC, Demirkan A, Janssens AC, Rice K, et al. (2012) Genome-Wide Profiling of Blood Pressure in Adults and Children. Hypertension 59: 241–247.HR TaalGC VerwoertA. DemirkanAC JanssensK. Rice2012Genome-Wide Profiling of Blood Pressure in Adults and Children.Hypertension59241247
- 5. Crough T, Khanna R (2009) Immunobiology of human cytomegalovirus: from bench to bedside. Clin Microbiol Rev 22: 76–98, Table of Contents. T. CroughR. Khanna2009Immunobiology of human cytomegalovirus: from bench to bedside.Clin Microbiol Rev 22: 76–98, Table of Contents
- 6. Bate SL, Dollard SC, Cannon MJ (2010) Cytomegalovirus seroprevalence in the United States: the national health and nutrition examination surveys, 1988–2004. Clin Infect Dis 50: 1439–1447.SL BateSC DollardMJ Cannon2010Cytomegalovirus seroprevalence in the United States: the national health and nutrition examination surveys, 1988–2004.Clin Infect Dis5014391447
- 7. Martin JM, Danziger-Isakov LA (2011) Cytomegalovirus risk, prevention, and management in pediatric solid organ transplantation. Pediatr Transplant 15: 229–236.JM MartinLA Danziger-Isakov2011Cytomegalovirus risk, prevention, and management in pediatric solid organ transplantation.Pediatr Transplant15229236
- 8. Simanek AM, Dowd JB, Pawelec G, Melzer D, Dutta A, et al. (2011) Seropositivity to cytomegalovirus, inflammation, all-cause and cardiovascular disease-related mortality in the United States. PLoS One 6: e16103.AM SimanekJB DowdG. PawelecD. MelzerA. Dutta2011Seropositivity to cytomegalovirus, inflammation, all-cause and cardiovascular disease-related mortality in the United States.PLoS One6e16103
- 9. Grahame-Clarke C, Chan NN, Andrew D, Ridgway GL, Betteridge DJ, et al. (2003) Human cytomegalovirus seropositivity is associated with impaired vascular function. Circulation 108: 678–683.C. Grahame-ClarkeNN ChanD. AndrewGL RidgwayDJ Betteridge2003Human cytomegalovirus seropositivity is associated with impaired vascular function.Circulation108678683
- 10. Kyto V, Vuorinen T, Saukko P, Lautenschlager I, Lignitz E, et al. (2005) Cytomegalovirus infection of the heart is common in patients with fatal myocarditis. Clin Infect Dis 40: 683–688.V. KytoT. VuorinenP. SaukkoI. LautenschlagerE. Lignitz2005Cytomegalovirus infection of the heart is common in patients with fatal myocarditis.Clin Infect Dis40683688
- 11. Grahame-Clarke C (2005) Human cytomegalovirus, endothelial function and atherosclerosis. Herpes 12: 42–45.C. Grahame-Clarke2005Human cytomegalovirus, endothelial function and atherosclerosis.Herpes124245
- 12. Danesh J, Collins R, Peto R (1997) Chronic infections and coronary heart disease: is there a link? Lancet 350: 430–436.J. DaneshR. CollinsR. Peto1997Chronic infections and coronary heart disease: is there a link?Lancet350430436
- 13. Zhang M, Yang Y, Yang X, Cai J (2011) Human cytomegalovirus infection is a novel etiology for essential hypertension. Med Hypotheses 76: 682–684.M. ZhangY. YangX. YangJ. Cai2011Human cytomegalovirus infection is a novel etiology for essential hypertension.Med Hypotheses76682684
- 14. Centers for Disease Control and Prevention (CDC). National Center for Health Statistics (NCHS): National Health and Nutrition Examination Survey questionnaire, examination protocol and laboratory protocol. Hyattsville, Maryland: United States Department of Health and Human Services, Centers for Disease Control and Prevention, 1999–2002. Centers for Disease Control and Prevention (CDC). National Center for Health Statistics (NCHS): National Health and Nutrition Examination Survey questionnaire, examination protocol and laboratory protocol. Hyattsville, Maryland: United States Department of Health and Human Services, Centers for Disease Control and Prevention, 1999–2002. Available: http://www.cdc.gov/nchs/nhanes.htm.Accessed 2011 Nov 30. Accessed 2011 Nov 30.
- 15. Cui JS, Hopper JL, Harrap SB (2003) Antihypertensive treatments obscure familial contributions to blood pressure variation. Hypertension 41: 207–210.JS CuiJL HopperSB Harrap2003Antihypertensive treatments obscure familial contributions to blood pressure variation.Hypertension41207210
- 16. Haarala A, Kahonen M, Lehtimaki T, Aittoniemi J, Jylhava J, et al. (2012) Relation of high cytomegalovirus antibody titres to blood pressure and brachial artery flow-mediated dilation in young men: the Cardiovascular Risk in Young Finns Study. Clin Exp Immunol 167: 309–316.A. HaaralaM. KahonenT. LehtimakiJ. AittoniemiJ. Jylhava2012Relation of high cytomegalovirus antibody titres to blood pressure and brachial artery flow-mediated dilation in young men: the Cardiovascular Risk in Young Finns Study.Clin Exp Immunol167309316
- 17. Shen YH, Zhang L, Utama B, Wang J, Gan Y, et al. (2006) Human cytomegalovirus inhibits Akt-mediated eNOS activation through upregulating PTEN (phosphatase and tensin homolog deleted on chromosome 10). Cardiovascular Research 69: 502–511.YH ShenL. ZhangB. UtamaJ. WangY. Gan2006Human cytomegalovirus inhibits Akt-mediated eNOS activation through upregulating PTEN (phosphatase and tensin homolog deleted on chromosome 10).Cardiovascular Research69502511
- 18. Weis M, Kledal TN, Lin KY, Panchal SN, Gao SZ, et al. (2004) Cytomegalovirus infection impairs the nitric oxide synthase pathway: role of asymmetric dimethylarginine in transplant arteriosclerosis. Circulation 109: 500–505.M. WeisTN KledalKY LinSN PanchalSZ Gao2004Cytomegalovirus infection impairs the nitric oxide synthase pathway: role of asymmetric dimethylarginine in transplant arteriosclerosis.Circulation109500505
- 19. Petrakopoulou P, Kubrich M, Pehlivanli S, Meiser B, Reichart B, et al. (2004) Cytomegalovirus infection in heart transplant recipients is associated with impaired endothelial function. Circulation 110: II207–212.P. PetrakopoulouM. KubrichS. PehlivanliB. MeiserB. Reichart2004Cytomegalovirus infection in heart transplant recipients is associated with impaired endothelial function.Circulation110II207212
- 20. Poole E, Atkins E, Nakayama T, Yoshie O, Groves I, et al. (2008) NF-kappaB-mediated activation of the chemokine CCL22 by the product of the human cytomegalovirus gene UL144 escapes regulation by viral IE86. J Virol 82: 4250–4256.E. PooleE. AtkinsT. NakayamaO. YoshieI. Groves2008NF-kappaB-mediated activation of the chemokine CCL22 by the product of the human cytomegalovirus gene UL144 escapes regulation by viral IE86.J Virol8242504256
- 21. Wong WT, Wong SL, Tian XY, Huang Y (2010) Endothelial Dysfunction: The Common Consequence in Diabetes and Hypertension. Journal of Cardiovascular Pharmacology 55: 300–307.WT WongSL WongXY TianY. Huang2010Endothelial Dysfunction: The Common Consequence in Diabetes and Hypertension.Journal of Cardiovascular Pharmacology55300307
- 22. Savoia C, Schiffrin EL (2007) Vascular inflammation in hypertension and diabetes: molecular mechanisms and therapeutic interventions. Clinical Science 112: 375–384.C. SavoiaEL Schiffrin2007Vascular inflammation in hypertension and diabetes: molecular mechanisms and therapeutic interventions.Clinical Science112375384
- 23. Wills MR, Carmichael AJ, Mynard K, Jin X, Weekes MP, et al. (1996) The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity, and T-cell receptor usage of pp65-specific CTL. J Virol 70: 7569–7579.MR WillsAJ CarmichaelK. MynardX. JinMP Weekes1996The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity, and T-cell receptor usage of pp65-specific CTL.J Virol7075697579
- 24. Fuhrmann S, Streitz M, Reinke P, Volk HD, Kern F (2008) T cell response to the cytomegalovirus major capsid protein (UL86) is dominated by helper cells with a large polyfunctional component and diverse epitope recognition. J Infect Dis 197: 1455–1458.S. FuhrmannM. StreitzP. ReinkeHD VolkF. Kern2008T cell response to the cytomegalovirus major capsid protein (UL86) is dominated by helper cells with a large polyfunctional component and diverse epitope recognition.J Infect Dis19714551458
- 25. Guzik TJ, Hoch NE, Brown KA, McCann LA, Rahman A, et al. (2007) Role of the T cell in the genesis of angiotensin II induced hypertension and vascular dysfunction. J Exp Med 204: 2449–2460.TJ GuzikNE HochKA BrownLA McCannA. Rahman2007Role of the T cell in the genesis of angiotensin II induced hypertension and vascular dysfunction.J Exp Med20424492460
- 26. Zampetaki A, Willeit P, Drozdov I, Kiechl S, Mayr M (2011) Profiling of circulating microRNAs: from single biomarkers to re-wired networks. Cardiovascular Research. A. ZampetakiP. WilleitI. DrozdovS. KiechlM. Mayr2011Profiling of circulating microRNAs: from single biomarkers to re-wired networks.Cardiovascular Research
- 27. Li S, Zhu J, Zhang W, Chen Y, Zhang K, et al. (2011) Signature microRNA expression profile of essential hypertension and its novel link to human cytomegalovirus infection. Circulation 124: 175–184.S. LiJ. ZhuW. ZhangY. ChenK. Zhang2011Signature microRNA expression profile of essential hypertension and its novel link to human cytomegalovirus infection.Circulation124175184
- 28. Cheng J, Ke Q, Jin Z, Wang H, Kocher O, et al. (2009) Cytomegalovirus infection causes an increase of arterial blood pressure. PLoS Pathog 5: e1000427.J. ChengQ. KeZ. JinH. WangO. Kocher2009Cytomegalovirus infection causes an increase of arterial blood pressure.PLoS Pathog5e1000427
- 29. Schieffer B, Luchtefeld M, Braun S, Hilfiker A, Hilfiker-Kleiner D, et al (2000) Role of NAD(P)H oxidase in angiotensin II-induced JAK/STAT signaling and cytokine induction. Circ Res 87: 1195–1201.Schieffer B, Luchtefeld M, Braun S, Hilfiker A, Hilfiker-Kleiner D, et al2000Role of NAD(P)H oxidase in angiotensin II-induced JAK/STAT signaling and cytokine induction.Circ Res8711951201