The Red Queen hypothesis can explain the maintenance of host and parasite diversity. However, the Red Queen requires genetic specificity for infection risk (i.e., that infection depends on the exact combination of host and parasite genotypes) and strongly virulent effects of infection on host fitness. A European crustacean (Daphnia magna) – bacterium (Pasteuria ramosa) system typifies such specificity and high virulence. We studied the North American host Daphnia dentifera and its natural parasite Pasteuria ramosa, and also found strong genetic specificity for infection success and high virulence. These results suggest that Pasteuria could promote Red Queen dynamics with D. dentifera populations as well. However, the Red Queen might be undermined in this system by selection from a more common yeast parasite (Metschnikowia bicuspidata). Resistance to the yeast did not correlate with resistance to Pasteuria among host genotypes, suggesting that selection by Metschnikowia should proceed relatively independently of selection by Pasteuria.
Citation: Auld SKJR, Hall SR, Duffy MA (2012) Epidemiology of a Daphnia-Multiparasite System and Its Implications for the Red Queen. PLoS ONE 7(6): e39564. https://doi.org/10.1371/journal.pone.0039564
Editor: Anna-Liisa Laine, University of Helsinki, Finland
Received: March 28, 2012; Accepted: May 28, 2012; Published: June 25, 2012
Copyright: © 2012 Auld et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The authors received funding from the National Science Foundation (DEB-0841679 and DEB-1053525 to MAD; DEB-0841817 to SRH) and from the Georgia Institute of Technology. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Genetic specificity between hosts and their parasites shapes the ecology and evolution of infectious disease , . Specificity occurs in numerous host-parasite systems, and arises when the exact pairing of host and parasite genotypes determines the success of infection (e.g. , , , , ). Genetic specificity has the potential to influence disease phenomena in at least two ways. First, it decreases the likelihood of invasion of parasites into genetically diverse host populations (i.e., the parasite will have a lower reproductive ratio R0 when all else is equal, ) because much of the host population resists infection. Indeed, genetically diverse populations often experience smaller disease epidemics , , , , . Second, specificity means that each parasite genotype only selects against a subset of host genotypes (and vice-versa). Therefore, specificity can drive Red Queen coevolutionary dynamics, where the frequencies of host and parasite genotypes cycle through time , . Such coevolutionary cycling can maintain genetic diversity in both host and parasite populations , .
The European freshwater crustacean Daphnia magna and its sterilizing bacterial parasite Pasteuria ramosa exemplify genetic specificity , . Here, specificity stems from a mechanism at the point of entry of Pasteuria – the physical attachment of spores to the host oesophageal wall , not from a systemic immune response , . If infection is successful, Pasteuria inflicts high virulence on infected hosts through sterilization , . Further, Pasteuria epidemics can become large , , , promoting rapid evolution in host populations . In theory, such a combination of genetic specificity, high parasite virulence, and large epidemics promote Red Queen oscillatory dynamics , , . As predicted by this theory, Red Queen dynamics emerge in the D. magna-Pasteuria system .
These results suggest that Pasteuria could maintain genetic diversity in other susceptible host species. Therefore, Pasteuria could act as a general catalyst of Red Queen-driven coevolution in freshwater systems. To test this possibility, we looked for genetic specificity for infection with Pasteuria in a natural North American host, Daphnia dentifera, using an established experimental design . We also tested for evidence of high virulence in infected hosts, with particular focus on fecundity (since Pasteuria castrates its European D. magna hosts: ). If Pasteuria's specificity and virulence effects are general, the European Daphnia – Pasteuria story may apply more broadly.
However, Red Queen dynamics could be disrupted by the presence of another, more dominant parasite species. For example, directional selection from a non-specific parasite may shape evolution in the host population more than negative frequency-dependent selection from a less prevalent parasite that exhibits genetic specificity with the host. This consideration is especially important in natural systems where most hosts are susceptible to multiple parasite species , . In D. dentifera populations, epidemics of the yeast Metschnikowia bicuspidata are usually larger and more frequent than Pasteuria epidemics , . Metschnikowia does not, however, exhibit genetic specificity with D. dentifera ; instead, variation in infection risk stems mainly from variation in host exposure to the parasite . Since the infection genetics differ between the two parasites, a host that resists Pasteuria may not necessarily resist Metschnikowia. Such parasite-specific resistance (sensu ) may disrupt or prevent Red Queen dynamics. To examine this possibility, we compared mean resistance of host genotypes to both parasites.
Materials and Methods
Daphnia dentifera is a cyclically parthenogenetic freshwater crustacean widespread in stratified North American lakes . It hosts numerous parasites , , two of which receive focus here: the bacterium Pasteuria ramosa and the yeast Metschnikowia bicuspidata. For both parasites, infection occurs when the host encounters transmission spores when filter-feeding , . With Pasteuria, infection also critically depends on attachment of cells to the oesophagus of the host; this step depends strongly on genotype in the Daphnia magna system . In both species, spore release follows death of the host (i.e., both are obligate killers: , ), however, the two parasites vary in terms of their effects on fecundity and survivorship. Although not previously studied in D. dentifera, Pasteuria completely sterilizes D. magna . In contrast, infection of both D. magna and D. dentifera by the yeast causes a smaller reduction in fecundity but sharply reduces survival of hosts , , . In D. magna, Metschnikowia has a larger impact on host survival than does Pasteuria .
The experiments used host and parasite cultures and previously established laboratory protocols. The six clonal genotypes of Daphnia (named H1, H4, H9, H29, H37 and H119) and five Pasteuria isolates (named A, B, C, D, and G) all originated from Midland Lake, Greene County, Indiana, USA. The five Pasteuria isolates were taken from five different infected hosts in 2010 (one isolate per host individual) and were propagated by feeding homogenized, infected hosts to a single host genotype (H119). These isolates likely consist of a mix of Pasteuria clones and represent ecologically relevant sub-populations of Pasteuria (the same as ). The Metschnikowia isolate originated from multiple infected hosts collected from Baker Lake, Barry County, Michigan, USA in 2003 and was propagated in a similar manner (on a single host genotype, the “Standard” genotype, also collected in Michigan). We used only this lab-based culture because Metschnikowia isolated from different lakes and in different years show no genetic variation in infectivity or virulence on D. dentifera . No specific permits were required for the collection of D. dentifera (which is not a protected or endangered species), but permission was obtained for access to lakes.
The experimental design involved a factorial manipulation of these hosts and parasites. We crossed the six host genotypes with seven parasite treatments (five Pasteuria isolates, one Metschnikowia isolate, and a sham-exposure control), replicated 15 times, for a total of 630 experimental units. Four of the Pasteuria replicates were lost due to accidental death after treatment exposure. Maternal lines consisted of individual Daphnia kept under favourable conditions (20°C and 16∶8 hour light/dark) in 40 mL of medium (50% Artificial Daphnia medium  and 50% filtered lake water); they received ample food (1×106 cells of the alga Ankistrodesmus falcatus daily). Experimental animals consisted of second clutch offspring of third-generation maternal lines and were also kept in 40 mL of medium and fed ample food. In the parasite exposure treatments, each neonate (<24 hour old) received a high dose of spores (Pasteuria: 2000 spores/mL; Metschnikowia: 500 spores/mL) produced by gently crushing and diluting infected hosts. These doses aimed to yield similar infection levels among parasite species (based on pilot data). Sham controls received 100 μL of a Daphnia solution (50 uninfected hosts ground in 10 ml nanopure water). Replicates were fed lower food (0.5×106 Ankistrodesmus algal cells) during exposure to increase spore uptake by hosts . Treatment exposure lasted 5 days. After the exposure, each host was changed into fresh medium and given ample food (1×106 Ankistrodesmus algal cells) again. Hosts were checked daily for reproduction and mortality for 25 days. Medium was refreshed every 2–3 days.
All analyses were performed using the statistical package R (http://www.r-project.org). We analysed infection risk (that is, the proportion of infected hosts) by testing the fixed effects of host genotype and parasite species using data from only parasite-exposed hosts (not sham-exposed hosts). We tested for genetic specificity of Pasteuria infection using the fixed effects of host genotype and parasite isolate. Both Generalized Linear Models (GLM) were estimated with a binomial error distribution, and significance of each treatment was evaluated with deviance tests (i.e., a comparison of full model versus the reduced model). However, in previous Daphnia-parasite studies, genotype is fitted as a fixed effect, a random effect or both (e.g. , , , ). Therefore, we also fitted GLMs with host and parasite genotype identity as random effects. In all cases, we obtained the same qualitative results. We also tested for a relationship between risk of infection from Metschnikowia and Pasteuria (averaged over the five isolates) for each host genotype using a Spearman rank correlation.
We examined the fitness consequences of infection by the two parasites using three metrics: production of host offspring (fecundity), host survival, and the instantaneous rate of host population growth, r (a composite measure of host fitness calculated using the Euler-Lotka equation). Fecundity was analysed by testing the fixed effects of infection status (infected or not), host genotype, and parasite species. Both host survival and r were analysed by testing the fixed effects of host infection status (infected or not) and parasite species. All of these tests used data from parasite-exposed hosts only; for r, the means of data from each infection category and parasite species for each host genotype were used.
Metschnikowia's capacity to disrupt Red Queen dynamics between D. dentifera and Pasteuria should be reduced if the host pays an activation cost of resistance to Metschnikowia (i.e., if hosts that successfully resist Metschnikowia have a lower fitness than hosts that were not exposed to the parasite.) We looked for evidence of an activation cost of resistance to Metschnikowia by comparing host fecundity, survival, and r in controls (sham-exposures) with hosts that were exposed to parasites but did not suffer infection (sensu , ). A Cox's proportional hazards analysis was used to test for activation costs in terms of survival, and Welch's two-sample t-tests were used to test for activation costs in terms of fecundity and r.
We found relatively low overall levels of infection by Pasteuria, as well as strong genotype specificity governing infections. This, combined with the likelihood that our parasite isolates contain mixtures of Pasteuria genotypes , , means we cannot be sure that individuals in the Pasteuria exposures who did not become infected where exposed to a parasite spore of an infectious genotype. Thus, we cannot test for activation costs of resistance to Pasteuria. In any case, activation costs of resistance to Pasteuria are unlikely to be evolutionarily important because Pasteuria is so virulent. Since it castrates its hosts, the cost of Pasteuria infection is so massive that any cost of resistance would have to be exceptionally high to limit Red Queen dynamics.
Fecundity across treatments was examined using a GLM fit with a quasipoisson error distribution (used due to over-dispersion of the fecundity data), and survival analyses used Cox's proportional hazards. Measures of r were squared to normalize their distribution prior to analysis with an ANOVA (with type III SS).
Genetic specificity for infection risk
Genetic specificity for infection risk arose at both a within-species level (for the bacterium Pasteuria) and between parasite species. Infection risk from Pasteuria depended on the specific combination of host and parasite genotypes (Figure 1), as evidenced by a host genotype x Pasteuria isolate interaction (Table 1). This interaction remained significant when we treated both host genotype and parasite isolate as random effects (χ 2 = 15.39, df = 3, p<0.01). As expected based on prior studies, host genotype strongly influenced infection risk from Metschnikowia (Figure 2; Table 1). Infection risk also depended on an interaction between host genotype and parasite species (Table 1). This interaction remained significant when host genotype was coded as a random effect (χ 2 = 22.81, df = 2, p<0.0001). There was, however, no correlation between a genotype's risk of infection by Metschnikowia and its overall risk of infection by Pasteuria (rs = −0.38, p = 0.46; Figure 2).
. The matrix shows the proportion of hosts infected for each combination of host genotype (rows) and parasite isolate (columns). Cells show infectivity of the five different Pasteuria isolates on each of the six host genotypes. There are five shading categories: 0% infection (white), 1–20%, 21–40%, 41–60%, and 61–100% (black). See Table 1 for statistical details.
rs = −0.38, p = 0.46. Each data point is the mean for each host genotype.
Infection with either parasite substantially reduced fecundity in infected hosts (Figure 3A). However, the extent of this virulence on host fecundity differed between the parasites (i.e., there was a significant infection status x parasite species interaction; Table 2): Pasteuria-infected hosts suffered a greater reduction in fecundity than those infected with Metschnikowia (Figure 3A). The impact of infection on fecundity did not vary across host genotypes (that is, there was no infection status-by-host genotype interaction; Table 2). Host survival also strongly depended on infection status and parasite species: Metschnikowia-infected hosts died much earlier than either Pasteuria-infected hosts or uninfected hosts (Table 2; Figure 3B). Finally, the instantaneous rate of population growth (r; our composite measure of fitness) depended on both infection status and parasite species: infection with either parasite reduced r, but Pasteuria infections reduced r more than Metschnikowia infections. Thus, Pasteuria was more virulent (Table 2; Figure 3C).
A: production of host offspring (fecundity) over 25 days; B: survival of hosts; and C: instantaneous rate of population growth of hosts (r), a composite measure of host fitness. Error bars denote one standard error. In panel B, “Metsch-exposed” indicates animals who were exposed to Metschnikowia, but who did not become infected. In all three panels, data were pooled across host genotypes and parasite isolates. Numbers of replicates are shown above bars; for r, the means of data for each host genotype from each infection category were used, resulting in lower sample sizes.
Costs of resistance to Metschnikowia
Host fecundity did not differ between hosts that were exposed to Metschnikowia but not infected and those in our control treatment (control, n = 90; Metschnikowia-exposed, n = 69; Welch's two-sample t = 0.45, df = 110.74, p = 0.65: Figure 3A); neither host survival (Cox's proportional hazards: χ 2 = 0.10, p = 0.75; Figure 3B) nor the instantaneous rate of host population growth, r (Welch's two-sample t = 0.14, df = 7.31, p = 0.89; Figure 3C) differed between these two groups (that is, Metschnikowia-exposed and control). Thus, there was no evidence for a fitness cost of mobilising resistance mechanisms against Metschnikowia (Figure 3).
Red Queen dynamics require two essential ingredients. The first is genetic specificity, where infection depends on the precise combination of host and parasite genotypes ; the second is high virulence , , where infection by parasites causes dramatic loss of host fitness. A canonical example of genetic specificity occurs in the European Daphnia magna-Pasteuria host-parasite system , . We found Pasteuria produces a similar pattern of specificity in a North American host, Daphnia dentifera: infection risk depended on both host genotype and parasite isolate (Figure 1). Also, just as in D. magna, Pasteuria castrates D. dentifera, thus greatly depressing fecundity (but not survival: Figure 3; , ). As a result, fitness is much reduced in Pasteuria-infected D. dentifera (Figure 3). Therefore, just as in the D. magna system, Pasteuria in this North American system might also maintain host genetic diversity, become adapted to locally common host genotypes , , and promote long-term coevolutionary Red Queen dynamics .
In addition to Pasteuria, D. dentifera populations often encounter Metschnikowia, a very different parasite. Infection of D. dentifera by Metschnikowia does not depend on the specific genotype to which it is exposed ; instead, genetic variation for host susceptibility stems from host variation in risk of exposure to the parasite . This may explain the absence of activation costs of resistance to Metschnikowia: hosts that resisted the parasite may have prevented it from passing the gut wall and stimulating a potentially costly immune response (sensu ).
Here, we found that virulence caused by Metschnikowia arose mainly through reduced host survival in D. dentifera (Figure 3B; similar to Metschnikowia infection in D. magna hosts: ). While Metschnikowia killed hosts relatively quickly, it reduced host fecundity much less than Pasteuria did (Figure 3A). As a result, Metschnikowia reduced per capita host fitness (r) to a lesser extent than Pasteuria (Figure 3C). Nevertheless, despite the lack of genetic specificity and lower virulence, Metschnikowia can still drive evolutionary change of hosts during epidemics. Indeed, large Metschnikowia epidemics can substantially reduce host population densities  and impose both directional and disruptive selection on host populations , .
Due to differences in specificity and virulence, these two parasites may exert different ecological impacts on host populations. Theory suggests that specificity can reduce invasion success of parasites; i.e., they will have a lower reproductive ratio R0 (especially when host populations are genetically diverse: ). Indeed genetically diverse host populations of Daphnia magna have smaller epidemics of Pasteuria , . Specificity may thus (partially) explain why Pasteuria epidemics occur more rarely and remain much smaller than epidemics of Metschnikowia in natural populations of D. dentifera . However, even though they are relatively small, Pasteuria epidemics may still be ecologically important: theory predicts that, all else being equal, parasites that strongly reduce fecundity (rather than survival) should reduce host population densities, even during small epidemics .
Correlations between host resistance to Pasteuria and Metschnikowia could either amplify or dampen Red Queen oscillations. A negative correlation would indicate antagonistic pleiotropy, where resistance to Pasteuria would come at a cost to resistance to Metschnikowia ; this could bolster Red Queen dynamics. Conversely, a positive correlation would imply that hosts might use a general mechanism to resist both parasite species. In this positive correlation case, directional selection for generally resistant hosts could erode genetic variation for resistance in host populations and squash Red Queen dynamics  – provided that this general resistance is not traded-off against another parasite-independent host trait . This study found no correlation between host resistance to the two parasites (Figure 2), probably because of very different underlying resistance mechanisms involved , . We acknowledge, however, that a more powerful design using more host genotypes could more comprehensively exclude the possibility of correlations between resistance to these two parasites. The lack of a correlation between resistance to Metschnikowia and Pasteuria suggests evolution of D. dentifera in response to the two parasites should proceed relatively independently. Thus, we do not expect Metschnikowia epidemics to amplify or dampen Red Queen dynamics between D. dentifera and Pasteuria.
In conclusion, Pasteuria could promote genetic diversity in multiple host species through host-parasite coevolution. Pasteuria exhibits strong genetic specificity and high virulence with its North American host, D. dentifera, just as it does with its European host, D. magna. Can Pasteuria then promote host diversity in North American lakes? We cannot yet fully answer that question with these or other data, but the D. dentifera-Pasteuria system in isolation certainly contains several of the essential ingredients of the Red Queen. Still, it seems likely that the larger epidemics of the more common Metschnikowia yeast may mean that parasite-mediated selection in D. dentifera is driven primarily by Metschnikowia, making Red Queen dynamics relatively rare. This possibility further highlights the need to consider other co-occurring parasites when exploring host-parasite coevolution.
K. Boatman, M. Shocket, and Z. Brown helped collected hosts and parasites. We thank Anna Moynihan and two anonymous reviewers for helpful comments on a previous version of the manuscript.
Conceived and designed the experiments: SKJRA MAD. Performed the experiments: SKJRA. Analyzed the data: SKJRA MAD. Contributed reagents/materials/analysis tools: SRH. Wrote the paper: SKJRA SRH MAD.
- 1. Schmid-Hempel P, Ebert D (2003) On the evolutionary ecology of specific immune defence. Trends in Ecology and Evolution 18: 27–32.P. Schmid-HempelD. Ebert2003On the evolutionary ecology of specific immune defence.Trends in Ecology and Evolution182732
- 2. Lambrechts L (2010) Dissecting the genetic architecture of host-pathogen specificity. PLoS Pathogens 6: e1001019.L. Lambrechts2010Dissecting the genetic architecture of host-pathogen specificity.PLoS Pathogens6e1001019
- 3. Wilfert L, Schmid-Hempel P (2008) The genetic architecture of susceptibility to parasites. BMC Evolutionary Biology 8: 187–195.L. WilfertP. Schmid-Hempel2008The genetic architecture of susceptibility to parasites.BMC Evolutionary Biology8187195
- 4. Salvaudon L, Heraudet V, Shykoff JA (2007) Genotype-specific interactions and the trade-off between host and parasite fitness. BMC Evolutionary Biology 7: 189–199.L. SalvaudonV. HeraudetJA Shykoff2007Genotype-specific interactions and the trade-off between host and parasite fitness.BMC Evolutionary Biology7189199
- 5. Luijckx P, Ben-Ami F, Mouton L, Du Pasquier L, Ebert D (2011) Cloning of the unculturable parasite Pasteuria ramosa and its Daphnia host reveals extreme genotype-genotype interactions. Ecology Letters 14: 125–131.P. LuijckxF. Ben-AmiL. MoutonL. Du PasquierD. Ebert2011Cloning of the unculturable parasite Pasteuria ramosa and its Daphnia host reveals extreme genotype-genotype interactions.Ecology Letters14125131
- 6. Lambrechts L, Halbert J, Durand P, Gouagna LC, Koella JC (2005) Host genotype by parasite genotype interactions underlying the resistance of anopheline mosquitoes to Plasmodium falciparum. Malaria Journal 4: 3.L. LambrechtsJ. HalbertP. DurandLC GouagnaJC Koella2005Host genotype by parasite genotype interactions underlying the resistance of anopheline mosquitoes to Plasmodium falciparum.Malaria Journal43
- 7. Carius HJ, Little TJ, Ebert D (2001) Genetic variation in a host-parasite association: Potential for coevolution and frequency-dependent selection. Evolution 55: 1136–1145.HJ CariusTJ LittleD. Ebert2001Genetic variation in a host-parasite association: Potential for coevolution and frequency-dependent selection.Evolution5511361145
- 8. Lively CM (2010) The effect of host genetic diversity on disease spread. American Naturalist 175: E149–E152.CM Lively2010The effect of host genetic diversity on disease spread.American Naturalist175E149E152
- 9. Ganz HH, Ebert D (2010) Benefits of host genetic diversity for resistance to infection depend on parasite diversity. Ecology 91: 1263–1268.HH GanzD. Ebert2010Benefits of host genetic diversity for resistance to infection depend on parasite diversity.Ecology9112631268
- 10. Altermatt F, Ebert D (2008) Genetic diversity of Daphnia magna populations enhances resistance to parasites. Ecology Letters 11: 918–928.F. AltermattD. Ebert2008Genetic diversity of Daphnia magna populations enhances resistance to parasites.Ecology Letters11918928
- 11. Dwyer G, Elkinton JS, Buonaccorsi JP (1997) Host heterogeneity in susceptibility and disease dynamics: tests of a mathematical model. American Naturalist 150: 685–707.G. DwyerJS ElkintonJP Buonaccorsi1997Host heterogeneity in susceptibility and disease dynamics: tests of a mathematical model.American Naturalist150685707
- 12. Zhu Y, Chen H, Fan J, Wang Y, Li Y, et al. (2000) Genetic diversity and disease control in rice. Nature 406: 718–722.Y. ZhuH. ChenJ. FanY. WangY. Li2000Genetic diversity and disease control in rice.Nature406718722
- 13. Meagher S (1999) Genetic diversity and Capillaria hepatica (Nematoda) prevalence in Michigan deer mouse populations. Evolution 53: 1318–1324.S. Meagher1999Genetic diversity and Capillaria hepatica (Nematoda) prevalence in Michigan deer mouse populations.Evolution5313181324
- 14. Woolhouse ME, Webster JP, Domingo E, Charlesworth B, Levin BR (2002) Biological and biomedical implications of the co-evolution of pathogens and their hosts. Nat Genet 32: 569–577.ME WoolhouseJP WebsterE. DomingoB. CharlesworthBR Levin2002Biological and biomedical implications of the co-evolution of pathogens and their hosts.Nat Genet32569577
- 15. Hamilton WD (1980) Sex versus non-sex versus parasite. Oikos 35: 282–290.WD Hamilton1980Sex versus non-sex versus parasite.Oikos35282290
- 16. Byers DL (2005) Evolution in heterogeneous environments and the potential of maintenance of genetic variation in traits of adaptive significance. Genetica 123: 107–124.DL Byers2005Evolution in heterogeneous environments and the potential of maintenance of genetic variation in traits of adaptive significance.Genetica123107124
- 17. Duneau D, Luijckx P, Ben-Ami F, Laforsch C, Ebert D (2011) Resolving the infection process reveals striking differences in the contribution of environment, genetics and phylogeny to host-parasite interactions. BMC Biology 9: 11.D. DuneauP. LuijckxF. Ben-AmiC. LaforschD. Ebert2011Resolving the infection process reveals striking differences in the contribution of environment, genetics and phylogeny to host-parasite interactions.BMC Biology911
- 18. Auld SKJR, Edel KH, Little TJ (2012) The cellular immune response of Daphnia magna under host-parasite genetic variation and variation in initial dose. Evolution Early View. SKJR AuldKH EdelTJ Little2012The cellular immune response of Daphnia magna under host-parasite genetic variation and variation in initial dose.Evolution Early View
- 19. Auld SKJR, Graham AL, Wilson PJ, Little TJ (2012) Elevated haemocyte number is associated with infection and low fitness potential in wild Daphnia magna. Functional Ecology 26: 434–440.SKJR AuldAL GrahamPJ WilsonTJ Little2012Elevated haemocyte number is associated with infection and low fitness potential in wild Daphnia magna.Functional Ecology26434440
- 20. Ebert D, Rainey P, Embley TM, Scholz D (1996) Development, life cycle, ultrastructure and phylogenetic position of Pasteuria ramosa Metchnikoff 1888: Rediscovery of an obligate endoparasite of Daphnia magna Straus. Philosophical Transactions of the Royal Society of London Series B-Biological Sciences 351: 1689–1701.D. EbertP. RaineyTM EmbleyD. Scholz1996Development, life cycle, ultrastructure and phylogenetic position of Pasteuria ramosa Metchnikoff 1888: Rediscovery of an obligate endoparasite of Daphnia magna Straus.Philosophical Transactions of the Royal Society of London Series B-Biological Sciences35116891701
- 21. Little TJ, Ebert D (2000) The cause of parasitic infection in natural populations of Daphnia (Crustacea: Cladocera): the role of host genetics. Proceedings of the Royal Society of London Series B-Biological Sciences 267: 2037–2042.TJ LittleD. Ebert2000The cause of parasitic infection in natural populations of Daphnia (Crustacea: Cladocera): the role of host genetics.Proceedings of the Royal Society of London Series B-Biological Sciences26720372042
- 22. Stirnadel HA, Ebert D (1997) Prevalence, host specificity and impact on host fecundity of microparasites and epibionts in three sympatric Daphnia species. Journal of Animal Ecology 66: 212–222.HA StirnadelD. Ebert1997Prevalence, host specificity and impact on host fecundity of microparasites and epibionts in three sympatric Daphnia species.Journal of Animal Ecology66212222
- 23. Duncan AB, Little TJ (2007) Parasite-driven genetic change in a natural population of Daphnia. Evolution 61: 796–803.AB DuncanTJ Little2007Parasite-driven genetic change in a natural population of Daphnia.Evolution61796803
- 24. Mitchell SE, Read AF, Little TJ (2004) The effect of a pathogen epidemic on the genetic structure and reproductive strategy of the crustacean Daphnia magna. Ecology Letters 7: 848–858.SE MitchellAF ReadTJ Little2004The effect of a pathogen epidemic on the genetic structure and reproductive strategy of the crustacean Daphnia magna.Ecology Letters7848858
- 25. Clay K, Kover PX (1996) The Red Queen Hypothesis and plant/pathogen interactions. Annual Review of Phytopathology 34: 29–50.K. ClayPX Kover1996The Red Queen Hypothesis and plant/pathogen interactions.Annual Review of Phytopathology342950
- 26. Howard RS, Lively CM (1994) Parasitism, mutation accumulation and the maintenance of sex. Nature 367: 554–557.RS HowardCM Lively1994Parasitism, mutation accumulation and the maintenance of sex.Nature367554557
- 27. Lively CM (1999) Migration, Virulence, and the Geographic Mosaic of Adaptation by Parasites. American Naturalist 153: S34–S47.CM Lively1999Migration, Virulence, and the Geographic Mosaic of Adaptation by Parasites.American Naturalist153S34S47
- 28. Decaestecker E, Gaba S, Raeymaekers JAM, Stoks R, Van Kerckoven L, et al. (2007) Host-parasite ‘Red Queen’ dynamics archived in pond sediment. Nature 450: 870–873.E. DecaesteckerS. GabaJAM RaeymaekersR. StoksL. Van Kerckoven2007Host-parasite ‘Red Queen’ dynamics archived in pond sediment.Nature450870873
- 29. Woolhouse ME, Taylor LH, Haydon DT (2001) Population biology of multihost pathogens. Science 292: 1109–1112.ME WoolhouseLH TaylorDT Haydon2001Population biology of multihost pathogens.Science29211091112
- 30. Rigaud T, Perrot-Minnot M-J, Brown MJF (2010) Parasite and host assemblages: embracing the reality will improve our knowledge of parasite transmission and virulence. Proceedings of the Royal Society of London Series-B Biological Sciences 277: 3693–3702.T. RigaudM-J Perrot-MinnotMJF Brown2010Parasite and host assemblages: embracing the reality will improve our knowledge of parasite transmission and virulence.Proceedings of the Royal Society of London Series-B Biological Sciences27736933702
- 31. Hall SR, Becker CR, Duffy MA, Cáceres CE (2011) Epidemic size determines population-level effects of fungal parasites on Daphnia hosts. Oecologia 166: 833–842.SR HallCR BeckerMA DuffyCE Cáceres2011Epidemic size determines population-level effects of fungal parasites on Daphnia hosts.Oecologia166833842
- 32. Duffy MA, Cáceres CE, Hall SR, Tessier AJ, Ives AR (2010) Temporal, spatial and between-host comparisons of patterns of parasitism in lake zooplankton. Ecology 91: 3322–3331.MA DuffyCE CáceresSR HallAJ TessierAR Ives2010Temporal, spatial and between-host comparisons of patterns of parasitism in lake zooplankton.Ecology9133223331
- 33. Duffy MA, Sivars-Becker L (2007) Rapid evolution and ecological host-parasite dynamics. Ecol Lett 10: 44–53.MA DuffyL. Sivars-Becker2007Rapid evolution and ecological host-parasite dynamics.Ecol Lett104453
- 34. Hall SR, Becker CR, Duffy MA, Cáceres CE (2010) Variation in resource acquisition and use among hosts can create key epidemiological tradeoffs. American Naturalist 176: 557–565.SR HallCR BeckerMA DuffyCE Cáceres2010Variation in resource acquisition and use among hosts can create key epidemiological tradeoffs.American Naturalist176557565
- 35. Decaestecker E, Vergote A, Ebert D, De Meester L (2003) Evidence for strong host clone-parasite species interactions in the Daphnia microparasite system. Evolution 57: 784–792.E. DecaesteckerA. VergoteD. EbertL. De Meester2003Evidence for strong host clone-parasite species interactions in the Daphnia microparasite system.Evolution57784792
- 36. Hebert PDN (1995) The Daphnia of North America: An Illustrated Fauna. Guelph, Ontario: Cybernatural Software. PDN Hebert1995The Daphnia of North America: An Illustrated Fauna.Guelph, Ontario: Cybernatural Software
- 37. Green J (1974) Parasites and epibionts of Cladocera. Transactions of the Zoological Society of London 32: 417–515.J. Green1974Parasites and epibionts of Cladocera.Transactions of the Zoological Society of London32417515
- 38. Hall SR, Sivars-Becker L, Becker C, Duffy MA, Tessier AJ, et al. (2007) Eating yourself sick: transmission of disease as a function of foraging ecology. Ecology Letters 10: 207–218.SR HallL. Sivars-BeckerC. BeckerMA DuffyAJ Tessier2007Eating yourself sick: transmission of disease as a function of foraging ecology.Ecology Letters10207218
- 39. Ebert D, Zschokke-Rohringer CD, Carius H-J (2000) Dose effects and density-dependent regulation of two microparasites of Daphnia magna. Oecologia 122: 200–209.D. EbertCD Zschokke-RohringerH-J Carius2000Dose effects and density-dependent regulation of two microparasites of Daphnia magna.Oecologia122200209
- 40. Ebert D, Lipsitch M, Mangin KL (2000) The effect of parasites on host population density and extinction: Experimental epidemiology with Daphnia and six microparasites. American Naturalist 156: 459–477.D. EbertM. LipsitchKL Mangin2000The effect of parasites on host population density and extinction: Experimental epidemiology with Daphnia and six microparasites.American Naturalist156459477
- 41. Hall SR, Tessier AJ, Duffy MA, Huebner M, Cáceres CE (2006) Warmer does not have to mean sicker: temperature and predators can jointly drive timing of epidemics. Ecology 87: 1684–1695.SR HallAJ TessierMA DuffyM. HuebnerCE Cáceres2006Warmer does not have to mean sicker: temperature and predators can jointly drive timing of epidemics.Ecology8716841695
- 42. Duffy MA, Hall SR (2008) Selective predation and rapid evolution can jointly dampen effects of virulent parasites on Daphnia populations. American Naturalist 171: 499–510.MA DuffySR Hall2008Selective predation and rapid evolution can jointly dampen effects of virulent parasites on Daphnia populations.American Naturalist171499510
- 43. Kluttgen BU, Dulmer U, Engels M, Ratte HT (1994) ADaM, an artificial freshwater for the culture of zooplankton. Water Research 28: 743–746.BU KluttgenU. DulmerM. EngelsHT Ratte1994ADaM, an artificial freshwater for the culture of zooplankton.Water Research28743746
- 44. Vale PF, Little TJ (2009) Measuring parasite fitness under genetic and thermal variation. Heredity 103: 102–109.PF ValeTJ Little2009Measuring parasite fitness under genetic and thermal variation.Heredity103102109
- 45. Auld SKJR, Scholefield JA, Little TJ (2010) Genetic variation in the cellular response of Daphnia magna (Crustacea: Cladocera) to its bacterial parasite. Proceedings of the Royal Society of London Series-B Biological Sciences 277: 3291–3297.SKJR AuldJA ScholefieldTJ Little2010Genetic variation in the cellular response of Daphnia magna (Crustacea: Cladocera) to its bacterial parasite.Proceedings of the Royal Society of London Series-B Biological Sciences27732913297
- 46. Prior NH, Washington CN, Housley JM, Hall SR, Duffy MA, et al. (2011) Maternal effects and epidemiological traits in a planktonic host-parasite system. Evolutionary Ecology Research 13: 401–414.NH PriorCN WashingtonJM HousleySR HallMA Duffy2011Maternal effects and epidemiological traits in a planktonic host-parasite system.Evolutionary Ecology Research13401414
- 47. Little TJ, Killick SC (2007) Evidence for a cost of immunity when the crustacean Daphnia magna is exposed to the bacterial pathogen Pasteuria ramosa. Journal of Animal Ecology 76: 1202–1207.TJ LittleSC Killick2007Evidence for a cost of immunity when the crustacean Daphnia magna is exposed to the bacterial pathogen Pasteuria ramosa.Journal of Animal Ecology7612021207
- 48. Ben-Ami F, Mouton L, Ebert D (2008) The effects of multiple infections on the expression and evolution of virulence in a Daphnia-endoparasite system. Evolution 62: 1700–1711.F. Ben-AmiL. MoutonD. Ebert2008The effects of multiple infections on the expression and evolution of virulence in a Daphnia-endoparasite system.Evolution6217001711
- 49. Labbé P, Vale PF, Little TJ (2010) Successfully resisting a pathogen is rarely costly in Daphnia magna. BMC Evolutionary Biology 10: 355.P. LabbéPF ValeTJ Little2010Successfully resisting a pathogen is rarely costly in Daphnia magna.BMC Evolutionary Biology10355
- 50. Ebert D, Carius HJ, Little T, Decaestecker E (2004) The evolution of virulence when parasites cause host castration and gigantism. American Naturalist 164: S19–S32.D. EbertHJ CariusT. LittleE. Decaestecker2004The evolution of virulence when parasites cause host castration and gigantism.American Naturalist164S19S32
- 51. Lively CM, Dybdahl MF (2000) Parasite adaptation to locally common host genotypes. Nature 405: 679–681.CM LivelyMF Dybdahl2000Parasite adaptation to locally common host genotypes.Nature405679681
- 52. Ebert D (1994) Virulence and local adaptation of a horizontally transmitted parasite. Science 265: 1084–1086.D. Ebert1994Virulence and local adaptation of a horizontally transmitted parasite.Science26510841086
- 53. Duffy MA, Housley Ochs J, Penczykowski RM, Klausmeier CA, Hall SR (2012) Ecological context influences epidemic size and parasite-mediated selection. Science 335: 1636–1638.MA DuffyJ. Housley OchsRM PenczykowskiCA KlausmeierSR Hall2012Ecological context influences epidemic size and parasite-mediated selection.Science33516361638
- 54. Duffy MA, Brassil CE, Hall SR, Tessier AJ, Cáceres CE, et al. (2008) Parasite-mediated disruptive selection in a natural Daphnia population. BMC Evolutionary Biology 8: 80.MA DuffyCE BrassilSR HallAJ TessierCE Cáceres2008Parasite-mediated disruptive selection in a natural Daphnia population.BMC Evolutionary Biology880
- 55. Anderson RM, May RM (1979) Population biology of infectious disease: Part 1. Nature 280: 361–367.RM AndersonRM May1979Population biology of infectious disease: Part 1.Nature280361367
- 56. Fritz RS, Simms EL (1992) Plant resistance to herbivores and pathogens. Chicago: University of Chicago Press. RS FritzEL Simms1992Plant resistance to herbivores and pathogens.Chicago: University of Chicago Press
- 57. Little TJ (2002) The evolutionary significance of parasitism: do parasite-driven genetic dynamics occur ex silico? Journal of Evolutionary Biology 15: 1–9.TJ Little2002The evolutionary significance of parasitism: do parasite-driven genetic dynamics occur ex silico?Journal of Evolutionary Biology1519
- 58. Mitchell-Olds T, Bradley D (1996) Genetics of Brassica rapa. 3. Costs of disease resistance to three fungal pathogens. Evolution 50: 1859–1865.T. Mitchell-OldsD. Bradley1996Genetics of Brassica rapa. 3. Costs of disease resistance to three fungal pathogens.Evolution5018591865