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Performance of an Adipokine Pathway-Based Multilocus Genetic Risk Score for Prostate Cancer Risk Prediction

  • Ricardo J. T. Ribeiro ,

    Affiliations Molecular Oncology Group-CI, Portuguese Institute of Oncology, Porto, Portugal, ICBAS, Abel Salazar Biomedical Sciences Institute, University of Porto, Porto, Portugal, Division of Cancer Epidemiology, Department of Oncology, McGill University, Montreal, Canada, LPCC–Portuguese League Against Cancer (NRNorte), Porto, Portugal

  • Cátia P. D. Monteiro,

    Affiliations Molecular Oncology Group-CI, Portuguese Institute of Oncology, Porto, Portugal, LPCC–Portuguese League Against Cancer (NRNorte), Porto, Portugal

  • Andreia S. M. Azevedo,

    Affiliations Molecular Oncology Group-CI, Portuguese Institute of Oncology, Porto, Portugal, LPCC–Portuguese League Against Cancer (NRNorte), Porto, Portugal

  • Virgínia F. M. Cunha,

    Affiliations Molecular Oncology Group-CI, Portuguese Institute of Oncology, Porto, Portugal, LPCC–Portuguese League Against Cancer (NRNorte), Porto, Portugal

  • Agnihotram V. Ramanakumar,

    Affiliation Division of Cancer Epidemiology, Department of Oncology, McGill University, Montreal, Canada

  • Avelino M. Fraga,

    Affiliations Molecular Oncology Group-CI, Portuguese Institute of Oncology, Porto, Portugal, ICBAS, Abel Salazar Biomedical Sciences Institute, University of Porto, Porto, Portugal, Urology Department, D. Pedro V Military Hospital, Porto, Portugal

  • Francisco M. Pina,

    Affiliation Urology Department, S. João Hospital, Porto, Portugal

  • Carlos M. S. Lopes,

    Affiliation ICBAS, Abel Salazar Biomedical Sciences Institute, University of Porto, Porto, Portugal

  • Rui M. Medeiros,

    Affiliations Molecular Oncology Group-CI, Portuguese Institute of Oncology, Porto, Portugal, ICBAS, Abel Salazar Biomedical Sciences Institute, University of Porto, Porto, Portugal, LPCC–Portuguese League Against Cancer (NRNorte), Porto, Portugal

  • Eduardo L. Franco

    Affiliation Division of Cancer Epidemiology, Department of Oncology, McGill University, Montreal, Canada

Performance of an Adipokine Pathway-Based Multilocus Genetic Risk Score for Prostate Cancer Risk Prediction

  • Ricardo J. T. Ribeiro, 
  • Cátia P. D. Monteiro, 
  • Andreia S. M. Azevedo, 
  • Virgínia F. M. Cunha, 
  • Agnihotram V. Ramanakumar, 
  • Avelino M. Fraga, 
  • Francisco M. Pina, 
  • Carlos M. S. Lopes, 
  • Rui M. Medeiros, 
  • Eduardo L. Franco


Few biomarkers are available to predict prostate cancer risk. Single nucleotide polymorphisms (SNPs) tend to have weak individual effects but, in combination, they have stronger predictive value. Adipokine pathways have been implicated in the pathogenesis. We used a candidate pathway approach to investigate 29 functional SNPs in key genes from relevant adipokine pathways in a sample of 1006 men eligible for prostate biopsy. We used stepwise multivariate logistic regression and bootstrapping to develop a multilocus genetic risk score by weighting each risk SNP empirically based on its association with disease. Seven common functional polymorphisms were associated with overall and high-grade prostate cancer (Gleason≥7), whereas three variants were associated with high metastatic-risk prostate cancer (PSA≥20 ng/mL and/or Gleason≥8). The addition of genetic variants to age and PSA improved the predictive accuracy for overall and high-grade prostate cancer, using either the area under the receiver-operating characteristics curves (P<0.02), the net reclassification improvement (P<0.001) and integrated discrimination improvement (P<0.001) measures. These results suggest that functional polymorphisms in adipokine pathways may act individually and cumulatively to affect risk and severity of prostate cancer, supporting the influence of adipokine pathways in the pathogenesis of prostate cancer. Use of such adipokine multilocus genetic risk score can enhance the predictive value of PSA and age in estimating absolute risk, which supports further evaluation of its clinical significance.


Prostate cancer is a complex and unpredictable disease, with risk being affected by advancing age, ethnic background and family history. Although the causes of prostate cancer are not yet fully understood, genetic variation influences disease risk [1]. Prostate cancer is usually accompanied by a rise in the concentration of serum PSA, which has been used for decades as a sensitive but poorly specific biomarker, and a controversial predictor of prostate cancer mortality [2], [3]. Many prostatic biopsies are unnecessary [4], which underscores the need for better prediction models with increased specificity to aid clinicians decide whether or not to recommend biopsy. Furthermore, this is especially relevant in men with mildly elevated PSA values (3–10 ng/mL), but where the risk for being diagnosed with prostate cancer is only about 20–25% [5]. After diagnosis, some cancers are indolent and cause no clinical problems, whereas others progress and may be fatal [6]. Therefore, it is important to search for biomarkers of aggressive clinical outcome. Genetic markers provide good candidates for such a role.

Single-nucleotide polymorphisms (SNPs) identified as loci associated with prostate cancer in genome-wide association studies (GWAS) are common but confer only small increases in risk and the mechanisms underlying their association with prostate cancer risk remain unknown [7], [8]. Recently, selected SNPs from GWAS were analyzed and converted into a genetic risk score, which was shown to reduce the number of biopsies although it did not discriminate aggressive cases [9].

The association between body mass and risk of prostate cancer is supported by meta-analyses that suggest increased risk of aggressive prostate cancer in the obese [10], and by studies using methods to estimate abdominal adiposity [11]. Recent work has focused on the role of adipokines and obesity-related molecules in the etiology of prostate cancer [12], [13]. Variants in genes encoding components of these pathways have been evaluated for prostate cancer risk and promising candidates have been identified [14], [15], [16], [17]. These candidate genes code for molecules found to be over- or under-expressed in obesity [18], [19], [20] and are involved in several biological mechanisms that modulate tumor proliferation, apoptosis, angiogenesis, motility, migration, and immunity [12], [21], i.e., traits that ultimately influence tumor behavior. Thus, common polymorphisms in adipokine pathways are plausible candidates that may help predict prostate cancer susceptibility. However, few studies have examined prostate cancer risk in the context of multi-loci SNPs in different adipokine pathways. In this report, we tested the hypothesis that SNPs in candidate genes involved in adipokine pathways may contribute to prostate cancer susceptibility and aggressiveness in a population of men referred for diagnostic surveillance. We also assessed the clinical utility of an adipokine genetic risk score to enhance the predictive value of age and PSA to predict high-risk individuals for screening and therapeutic management.


A total of 449 histologically confirmed prostate cancer and 557 non-prostate cancer patients were included in the analyses. Prostate cancer patients were older (P<0.0001) and presented with significantly higher circulating levels of PSA and a lower free/total PSA ratio (P<0.0001 and P<0.0001, respectively) (Table 1).

We evaluated the associations between each individual SNP on prostate cancer susceptibility (Table S2). In the dominant effect models (referent: wild-type homozygote) there were significant decreases in risk for LEPR Gln223Arg (aOR = 0.6, 95%CI: 0.5–0.8, aOR = 0.6, 95%CI: 0.5–0.8 and aOR = 0.5, 95%CI: 0.4–0.8, for all, high-grade and high-risk prostate cancer for metastasis, respectively) and for FGF2+223 C>T (aOR = 0.7, 95%CI: 0.5–1.0 in high-grade prostate cancer). An increase in risk of high-grade prostate cancer was found in carriers of the IL6R Asp358Ala variant (aOR = 1.3, 95%CI: 1.0–1.7). In the recessive effect models (referent: wild-type homozygotes and heterozygotes) a significantly increased risk was observed for IGF1R+3174 G>A (aOR = 1.3, 95%CI: 1.0–1.9 for overall prostate cancer), IGFBP3-202 A>C (aOR = 1.3, 95%CI: 1.0–1.8 and aOR = 1.3, 95%CI: 1.0–1.8, for overall and high-grade prostate cancer, respectively) and with SPP1-66 T>C (aOR = 1.8, 95%CI: 1.1–3.0, aOR = 1.9, 95%CI: 1.1–3.2 and aOR = 2.4, 95%CI: 1.2–4.8, in overall, high-grade and high-risk prostate cancer for metastasis, respectively). Likewise, a significant protective effect for high-grade prostate cancer was observed for carriers of the IL6-597 G>A variant (aOR = 0.7, 95%CI: 0.4–1.0). Age-stratification on the aforementioned seven SNPs indicated that effects were mostly restricted to subjects below the median age (of non-cancer group, Table S3).

Figure 1 shows that among prostate cancer cases there was a shorter waiting time-to-onset in IL6R Asp358Ala C-allele carriers (P = 0.026) and in IGF1R+3174 AA homozygous (P = 0.002). None of the other five SNPs influenced the time to onset of disease (data not shown).

Figure 1. Kaplan Meier analyses plots of significant genetic polymorphisms.

(A) IL6R D358A A>C and (B) IGF1R+3174 G>A. In figure 1A the dashed line corresponds to AA and the dotted line to CC/CA genotype. In figure 1B the dashed line represents AA, whereas the solid corresponds to GG/GA genotype. The Log Rank test was used to compare genotypes in IL6R D358A A>C (P = 0.026) and IGF1R+3174 G>A (P = 0.002).

To test our hypothesis that genetic variability in SNPs from adipokine pathways may contribute a combined effect for prostate cancer risk and/or aggressiveness, we estimated the overall mutually-adjusted effects by stepwise multivariate logistic regression. The SNPs in LEPR Gln223Arg, SPP1-66 T>G, IGF1R+3174 G>A, IGFBP3-202 A>C, FGF2+223 C>T and IL6-597 G>A, plus age and PSA remained independently associated with risk for overall, and for high-grade prostate cancer (Table 2). In the prostate cancer group with high risk for metastasis, only the LEPR Gln223Arg, SPP1-66 T>G and FGF2+223 C>T genetic variants, age and PSA persisted (Table 2). Within all groups, bootstrap analysis confirmed results (Table 2).

Table 2. Stepwise multivariate logistic regression and Bootstrap analyses.

The inclusive (age and PSA added to the multi-locus genetic set) linear risk scores computed on the basis of the above logistic regression models were tested as overall risk predictors categorized in tertiles based on the distribution in the non-prostate cancer group. As shown in Table 3, the risk for prostate cancer and high-grade prostate cancer increased according to the tertile of risk score (Ptrend <0.0001 for both outcome categories). The age-adjusted ORs for unit changes in the inclusive risk score were 2.52 (95%CI: 2.0–3.2) and 2.77 (95%CI: 2.2–3.5) for all prostate cancers and high-grade prostate cancers, respectively. The goodness of fit for the logistic regression models based on the inclusive score were significantly greater than for the models based on the restricted age plus PSA score, for all prostate cancers (P = 0.0002) and high-grade prostate cancers (P = 0.0001), after likelihood ratio test.

Table 3. Tertiles of inclusive genetic risk score (GRS) and age-adjusted OR (CI 95%) for prostate cancer.

Figure 2 shows the ROC curves for the all-inclusive genetic risk score and for the age and PSA-based risk score. The AUC estimates for both outcomes (all prostate cancers and high-grade prostate cancers) were significantly higher for the all-inclusive score than with the age plus PSA predictor, P = 0.0099 and P = 0.0196, respectively (Figure 2). The statistically superior predictive value of the all-inclusive score was confirmed via the NRI (all prostate cancers: 9.5%, P<0.0001, high-grade prostate cancer: 13.3%, P<0.0001) and IDI (all prostate cancers: 0.021, P<0.0001, high-grade prostate cancer: 0.024, P<0.0001) comparisons.

Figure 2. ROC curves and AUC for the inclusive risk score and PSA plus age alone.

(A) All prostate cancer and (B) restricted to high-grade prostate cancer. Solid line corresponds to the all inclusive score, whereas dashed line represents the PSA and age risk score. The dotted line indicates the behavior of a hypothetical random score. The Likelihood ratio test was used to estimate the superiority of the inclusive risk score relative to that of the age+PSA score for all prostate cancer (inclusive: AUC = 0.6806, PSA and age: AUC = 0.6476, P = 0.0002) and high-grade prostate cancer (inclusive: AUC = 0.7119, PSA and age: AUC = 0.6808, P = 0.0001). PSA, prostate specific antigen.

Genotype distributions in four SNPs deviated from Hardy-Weinberg equilibrium (Table S1). In sensitivity analysis of three relevant SNPs, equilibrium was achieved after restricting the control group to constrained conditions, whereas the trend towards increased risk remained stable, regardless of control group used (restricted or unrestricted) (Table S4). Three of these four deviated SNPs ended up in the all-inclusive risk score. Therefore, as an additional step to clarify the relative importance of these SNPs we tested a four SNP risk score (excluding the 3 SNPs that were not in equilibrium). Findings showed that the predictive and discriminative ability of the inclusive risk score based on 4 SNPs remained significant (data not shown). Therefore, we used the all inclusive score.


Adipose tissue deregulation has been proposed as a relevant mechanism underlying obesity-related cancer, due to inappropriate release of biologically active adipokines. Thus, functional SNPs in genes coding for molecules involved in adipokine pathways may modulate the expression, transport, or signaling of adipokines, thereby influencing prostate cancer risk and biology. Our findings show that SNPs in genes from adipokine pathways (leptin, interleukin-6, fibroblast growth factor 2, osteopontin, and insulin growth factor) may influence the development of prostate cancer and aggressive disease. Interestingly, we found that both the LEPR Gln223Arg homozygous A and SPP1-66 homozygous G were significantly associated with all outcomes (risks of overall, high-grade, and high metastatic-risk prostate cancers).

The pleiotrophic effects of leptin, namely in tumor development and progression are mediated by its receptor [12], [13]. Studies of SNPs affecting this pathway provided inconsistent results in prostate cancer. The leptin SNP at position -2548 was proposed as a susceptibility locus for prostate cancer [14], [15], albeit our data do not support this contention. Conversely, we found an increased risk in LEPR Gln223Arg homozygous A for prostate cancer, whereas others observed no such association [14], [16]. LEPR Gln223Arg AA carriers have lower leptin binding affinity to soluble leptin receptor and have increased circulating free leptin and soluble leptin receptor levels [22], [23]. Therefore, there is increased availability of leptin for binding to the long leptin receptor signaling isoform in the prostate tumor cell membrane. Cumulatively, the aminoacid change in this SNP may influence the signal for receptor intracellular recycling or degradation [24], modulating the availability of membrane-bound leptin receptor in tumor cells.

Osteopontin is a cytokine-like extracellular matrix molecule, that influences cell migration and anti-apoptosis in cancer [25]. This molecule has been implicated in aggressive and metastatic disease, and is one of a four-gene signature in prostate cancer that predicts metastasis and death [26], [27]. The T-to-G substitution at position -66 in the human SPP1 gene modulates promoter activity [28]. The modified bioavailability of osteopontin may induce TH1-to-Th2 shift, modulating the microenvironment [28], and tumor development.

The IGF1-mediated activation of IGF1R has been demonstrated to contribute to tumor progression [29]. The IGF binding proteins modulate the effects of IGF1 and its biological function in different tissues. Recent evidence indicates increased risk of prostate cancer in individuals with high serum IGF1 levels, whereas risk was decreased in those with high levels of IGFBP-3 [30]. Furthermore, it was also found that the IGFBP3-202 A>C SNP was associated with prostate cancer and with low circulating levels of IGFBP3 [30]. The present study corroborates previous findings on the IGFBP3-202 A>C CC genotype risk for prostate cancer and high-grade disease [30], [31]. Cumulatively, functional studies confirmed the underexpression of IGFBP3 in C-allele carriers [32], resulting in increased IGF1 bioavailability. Signaling through the IGF1R is required for growth and survival [29]. The synonymous IGF1R SNP at locus +3174 was described as a possible splicing regulator [33], thereby generating protein diversity [34] and serving as a mechanism for modulating gene expression [35]. Our findings showing that AA carriers remained independently associated with risk for all and for high-grade prostate cancer, suggest that this SNP may modulate IGF1R cell surface protein quantity, as well as IGF1R/IGF1R internalization and degradation, consequently influencing prostate tumor growth. Insulin receptor substrate –1 (IRS1) is the primary docking protein of IGF1R, which mediates PI3K pathway activation within the IGF1/IGF1R system. Although the IRS1 Gly972Arg SNP results in structural protein differences [36] in our study this SNP was not associated with prostate cancer risk, confirming previous findings [37].

FGF2 may have a role in tumorigenesis and cancer progression through induction of angiogenesis [38]. The FGF+223 variant in exon 1 is associated with FGF2 expression at the transcriptional and translational level [39]. Our findings show increased risk for all, high-grade, and high-metastasis risk prostate cancer among CC carriers, which are coherent with a functional upregulation of FGF2. This molecule interacts with a family of four distinct, high-affinity tyrosine kinase receptors, FGFR 1–4. Although increased availability of FGF2 and changes in FGFR2 receptor availability could play a role in the initiation and progression of prostate cancer, we did not find an association between the FGFR2 rs2981582 in exon 2 and prostate cancer.

Initiation and progression of prostate cancer are stimulated by IL-6 [40]. Previous findings reported no association of the IL6-174 G>C SNP with prostate cancer [17], [41], except for a small study of aggressive disease risk [42]. We did not find an association for the IL6-174 G>C SNP and prostate cancer. On the other hand, we found that carriers of the IL6-597 G-allele were at increased risk for high-grade prostate cancer. In fact, functional SNPs in the promoter region of IL6 (-174, -572 and -597) do not act independently in the regulation of IL6 transcription [43]. The GG genotype in IL6-597 is linked to the GG genotype in IL6-174, which is associated with increased IL6 mRNA and protein levels. Therefore, the higher risk of high-grade prostate cancer associated with the IL6-597 G-allele may be due to increased IL6. IL6 signals are transmitted via a heterodimeric receptor complex consisting of a soluble interleukin-6 alpha subunit and a membrane-bound signal-transducing subunit, IL6ST. The common IL6R Asp385Ala variant is responsible for serum levels of soluble IL6R and IL6 and associates with IL6R membrane binding due to altered cleavage site [44], therefore, explaining our findings. The predominant activation of trans-signaling IL6/soluble IL6R pathway in aggressive prostate cancer [45], together with the functional IL6R Asp358Ala influence in this mechanism, supports the increased risk for high-grade prostate cancer we observed for C carriers (Ala carriers).

Several of the candidate SNPs in adipokine pathways known to affect oncogenesis, investigated here, were not associated with prostate cancer risk. Most of our null results for candidate SNPs in ADIPOQ+276, VEGF-460, VEGF+405, VEGF+936, PPARG Pro12Ala and TNF-308, are in agreement with other studies [14], [17], [46], [47]. To our knowledge, there have been no prior reports of null associations of KDR-604, PPARD-87, PPARGC1A Gly482Ser, TNFRSF1A-329, ADIPOQ+45, ADIPOQ-11426, IL6ST Gly148Arg, IL6-6331, and TNF-863 functional SNPs with prostate cancer.

We observed that some SNPs have a significant risk effect mainly in younger ages. The all-life exposure to increased levels of adipokines and pathway activation may influence early development of prostate cancer. Furthermore, IL6R Asp358Ala and IGF1R +3174 SNPs were significantly associated with early-onset prostate cancer, possibly due to accelerated tumor formation.

We tested each SNP for association with two clinically-relevant definitions of unfavorable outcomes: high-grade (combined Gleason score ≥7) and high-metastasis risk (combined Gleason score ≥8 and/or PSA≥20 ng/mL) prostate cancers. Combined Gleason score is a powerful predictor of disease progression and mortality [48], whereas Gleason score ≥8 is associated with aggressive biological behavior and increased risk of occult disseminated disease [49]. We found functional variants in genes from leptin, osteopontin, insulin growth factor, fibroblast growth factor 2 and interleukin 6 pathways to be related with high-grade prostate cancer, while SNPs in the leptin, osteopontin and fibroblast growth factor 2 axis associate with high-metastasis risk prostate cancer. These pathways are known to be involved in aggressive prostate cancer, lending support for these SNPs as clinical markers of aggressive disease. The SNPs in the risk score predict high grade/aggressive disease, but they also predict overall prostate cancer risk. The ability to predict overall as well as high grade cancers might be due to the significant proportion of high grade prostate cancer (Gleason≥7) (83%) in our cancer population.

Although a wealth of evidence demonstrates the effects of individual adipokines on prostate carcinogenesis, it is unlikely that the overall pathophysiological impact is due to the influence of a single adipokine in vivo. We showed that consideration of the cumulative susceptibility contributed by SNPs from adipokine pathways helps in risk stratification. Our analyses indicate that the inclusive (age and PSA added to the multi-locus genetic set) risk score provides improvements in discrimination and prediction of all prostate cancer, and high-grade prostate cancer. We suggest that risk genotypes in the inclusive model may cooperate to influence the endocrine and paracrine activity of adipokine pathways that leads to tumor development and progression. However, the mechanisms underlying these high-order interactions among genetic polymorphisms in adipokine pathways genes in modulating prostate cancer risk remain to be fully elucidated.

In this cohort of men subjected to prostate biopsy due to abnormal clinical and/or PSA findings where an extensive biopsy scheme was used, we showed that by adding a genetic score based on 7 SNPs significantly improved the discriminative ability of an established parsimonious model with only PSA and age. The AUC increased significantly from 0.65 to 0.68 for all prostate cancer and from 0.68 to 0.71 in high grade prostate cancer, when the genetic variants were added to the model. Furthermore, the improved predictive value of the score for prostate cancer risk persisted with a four SNPs risk score (excluding SNPs deviated from Hardy-Weinberg equilibrium). Although we present the largest effort to date to study the association between adipokine genetic risk score and risk of prostate cancer, our results should be interpreted in the context of several potential limitations. We took a focused candidate gene approach to evaluate key SNPs in adipokine pathways but our SNP panel could be incomplete. Likewise, several newly reported prostate cancer risk-associated SNPs from genome-wide association studies were not included in the risk prediction model. Had we been able to include them, the overall risk prediction might have improved. We also estimated risk associations in this study population with an exploratory intent, without having the opportunity to validate our findings in a separate sample of patients undergoing prostate cancer screening. Therefore, further studies in independent populations are required. Finally, despite our relatively large sample size, we had limited statistical power to examine genetic variants in relation to high-metastasis risk prostate cancer, because of the small number of cases in this group. However, our study has several strengths: i) it was prospective and large enough for key outcomes of interest, ii) most of the genes and SNPs selected were based on biological evidence of functional importance; iii) study design and statistical analyses accounted for relevant risk factors such as ethnicity and age [50], and although we did not have data on heredity information in a large set of subjects, only 2.2% were actually younger than 55 years of age, suggesting that hereditary prostate cancers were rare in our sample; iv) we used statistical strategies to assess the robustness of associations, such as bootstrap resampling and discrimination improvement measures; and v) all men were screened for prostate cancer based on both PSA level and digital rectal exam during the recruitment period and diagnosis was determined by standard biopsy, thus making outcome misclassification unlikely.

In summary, we identified SNPs in adipokine pathways that are associated with prostate cancer development and with a more aggressive phenotype. The inclusion of SNPs in the risk score model significantly improved, albeit modestly, the performance of PSA and age to predict overall prostate cancer and high-grade prostate cancer risk in men subjected to biopsy. The inclusion of further functional SNPs in a susceptibility model for prostate cancer is warranted, in order to determine a multi-locus model to accurately predict prostate cancer and disease aggressiveness. The use of improved risk models, such as the one described here, may impact public health strategies if shown to have clinical utility when combined with individualized screening and risk reduction strategies.

Materials and Methods

Ethics Statement

This study was approved by the ethics committees of Porto Military Hospital and São João Hospital (Porto, Portugal). Patients were included after signing a written informed consent.


Participants were enrolled between September 2007 and October 2010, after being referred to the urology departments of the participating hospitals for prostatic transrectal ultrasound guided biopsy (8–13 cores), on the basis of abnormal digital rectal examinations and/or single baseline PSA levels over 2.5 ng/mL. Our study population consisted of 1099 consecutively-admitted Caucasian men who had histological evaluation and consented for genotyping.

We selected a control group of patients with non-prostate cancer (benign prostate hyperplasia [BPH] or chronic prostatitis) from the prospectively enrolled men undergoing prostate biopsy. Our choice of this control group was based on the following reasons: (i) diagnosis was contemporary with that of cancers; (ii) their advanced age at diagnosis allowed matching with elderly cancer patients; (iii) all patients underwent digital rectal examination, PSA testing and prostate needle biopsy, making the possibility of crossover remote. Most men develop BPH or chronic prostatitis by the 7th–8th decades of life, making it normal in men of that age to carry benign prostatic disease. This permitted our control group subjects to have comparable ages to those of our prostate cancer patients, thus minimizing the likelihood of outcome misclassification. Had we restricted controls to men without prostatic disease there would have been a severe imbalance in age distributions, which would introduce bias.

Prostate pathology and Gleason scores were determined via biopsy. In re-biopsed individuals only the last, most relevant pathological diagnosis was considered. Ninety-three men were excluded from the study due to a pathology report of high-grade prostatic intraepithelial neoplasia or a biopsy suspicious of cancer only. None of the participants had undergone prostate cancer treatment (hormonal castration, surgery, chemotherapy, or radiotherapy). All remaining 1006 eligible patients were included for molecular analysis.

Genetic Variants and Genotyping

Candidate SNPs were selected from the best evidence from published studies and through public databases that provide information on the phenotypic risks. Candidate genes involved in adipokine pathways known to affect oncogenesis were selected. SNPs with minor allele frequencies <0.05 were excluded. A total of 29 literature-defined putative functional SNPs in 19 different genes were selected, corresponding to 9 adipokine pathways (Table S1).

Genotyping for 22 SNPs (two in ADIPOQ, IL6, IL6R, KDR, three in VEGF, LEP, two in LEPR, PPARG, PPARGC1A, PPARD, SPP1, IGF1R, IGFBP3, IRS1, FGF2, FGFR2, TNF, TNFRSF1A) was performed using TaqMan allelic discrimination (Applied Biosystems), whereas 7 SNPs were genotyped through polymerase chain reaction - restriction fragment length polymorphism analysis (IL6-597/−572/−174, ADIPOQ+45, IL6ST Gly148Arg, LEPR Gln223Arg and TNF-863), using previously described protocols. For quality control we used non-template controls in all runs and blind replicate genotype assessment in 5% of the samples. For the majority of SNPs, we observed almost complete concordance among duplicates.

Statistical Analysis

The Mann-Whitney test was used to compare means between prostate cancer and non-cancer groups. The chi-square test was used to test for departures from Hardy-Weinberg equilibrium for each SNP based on the distribution among the non-prostate cancer group.

Unconditional logistic regression was used to estimate age-adjusted odds ratios (aORs) and 95% confidence intervals (95%CIs) for the associations between the polymorphisms and development of prostate cancer based on both recessive and dominant models. We examined the association of genetic markers with overall prostate cancer, restricted to high-grade prostate cancer (combined Gleason score ≥7), and restricted to high-risk prostate cancer for metastasis (PSA at diagnosis ≥20 ng/mL and/or combined Gleason score ≥8). Sensitivity analyses were conducted on the risk-associated SNPs that exhibited deviation from Hardy-Weinberg equilibrium. This was done by restricting the non-prostate cancer group to normal/BPH histology, and with serum PSA <4 ng/mL and then retesting the risk associations and departure from Hardy-Weinberg equilibrium.

To assess whether risk-associated SNPs affected time to clinical onset of disease we constructed Kaplan-Meier plots of the cumulative probabilities for having prostate cancer diagnosed at different ages according to each SNP. This analysis was conducted among prostate cancer cases only.

Stepwise multivariate logistic regression with backward elimination (P-value for retention = 0.15) was conducted in SNPs with aOR ≤0.7 or aOR ≥1.3 (30% decrease or increase in odds of the outcome) plus age and PSA as continuous variables. Bootstrapping analyses were performed through MonteCarlo simulation (1000 replications).

We constructed an inclusive multi-locus genetic risk score for each participant by summing the coefficients for each of the resulting variables after stepwise regression analyses. For each SNP, the risk genotypes were coded as 1 and the non-risk alleles as 0. The model was determined by multiplying the β coefficient by the SNPs, plus the γ coefficient by the PSA value and the α coefficient by the patient’s age (Inclusive Risk Score  = Σ βi x Xi+γ x PSA+α x Age; where Xi = SNPs scaled for risk, βi = coefficient for SNPs, γ = coefficient for PSA, α = coefficient for Age). A parsimonious risk score was calculated based on a model that included only PSA and age at diagnosis. These models were fitted independently using all prostate cancers and then restricted to high-grade prostate cancers as outcomes. A likelihood-ratio test was used to assess the goodness of fit between the two logistic regression models.

We assessed the clinical value of the above two scores in correctly predicting disease status by receiver operating characteristic (ROC) curve analysis. We compared the areas under the ROC curves (AUC) constructed with both scores (with and without genetic information), both for all prostate cancers and high-grade cancers, using a non-parametric algorithm [51].

We evaluated the improvement in model performance (PSA and age risk score) introduced by the inclusion of the SNPs risk information, using the net reclassification improvement (NRI) and the integrated discrimination improvement (IDI) tests [52], [53]. The NRI measures the reclassification of men from one risk category to another by addition of the genetic information to the PSA and age prediction model, and the extent of clinical utility can be evaluated by the magnitude of the NRI. The IDI does not consider risk thresholds; rather it is the mean of increments and decrements in estimated probabilities of prostate cancer for cases and non cases, comparing models. Since the NRI measurement is heavily dependent on the threshold levels used, we used a threshold probability between 15% and 45%, similar to those previously reported in such clinical context [54].

All statistical analyses were conducted in STATA version 10.0 (StataCorp, College Station, Texas). For NRI and IDI calculations, we used the nriidi-package for Stata 11 [53].

Supporting Information

Table S1.

Characteristics of candidate Single Nucleotide Polymorphisms (SNPs) involved in adipokine pathways potentially associated with cancer. HW-E, Hardy-Weinberg Equilibrium; ADIPOQ, adiponectin gene; IL6, interleukin-6 gene; IL6R, interleukin-6 receptor gene; IL6ST, interleukin-6 signal transducer gene; KDR, vascular endothelial growth factor receptor 2 gene; VEGF, vascular endothelial growth factor gene; LEP, leptin gene; LEPR, leptin receptor gene; PPARGC1A, Peroxisome proliferator-activated receptor gamma co-activator 1 alpha gene; PPARD, Peroxisome proliferator-activated receptor delta gene; PPARG, Peroxisome proliferator-activated receptor gamma gene; SPP1, osteopontin gene; IRS1, insulin receptor substrate 1 gene; IGFBP3, insulin growth factor binding protein 3 gene; IGF1R, insulin growth factor 1 receptor gene; FGF2, fibroblast growth factor 2 gene; FGFR2, fibroblast growth factor receptor 2 gene; TNF, tumoral necrosis factor alpha gene; TNFRSF1A, tumoral necrosis factor receptor 1 gene. a The percentage of successfully genotyped DNA samples from the 1006 participants.



Table S2.

Age-adjusted Odds Ratios and 95%CI of prostate cancer (PCa) according to adipokine pathways polymorphisms. N, number of evaluable patients; SNP, single nucleotide polymorphism; OR (95%CI), age-adjusted odds-ratio and respective 95% confidence interval. a HGPCa,High-grade Prostate Cancer (Gleason grade ≥7). b HRPCaM, High-risk Prostate Cancer for metastasis (Gleason grade ≥8 and/or PSA ≥20 ng/mL).



Table S3.

Age-adjusted Odds Ratios and 95%CI for prostate cancer (PCa) associated with selected SNPs, after age stratification. a High-grade Prostate Cancer, Gleason grade ≥7; b High-risk Prostate Cancer for metastasis, Gleason grade ≥8 and/or PSA ≥20 ng/mL; aOR (95%CI), age-adjusted odds ratio and respective 95% Confidence Interval; PCa, Prostate Cancer; Median age at diagnosis = 67.5 years; *Evaluable individuals for analysis.



Table S4.

Sensitivity analysis in SNPs with deviation from Hardy-Weinberg equilibrium. Risk for prostate cancer after restriction on the non-prostate cancer group to just benign prostate hyperplasia and normal or to PSA below 4 ng/mL. *Hardy-Weinberg equilibrium, Pearson chi-square analysis for differences between observed and expected genotype frequencies; **Age-adjusted odds ratios; BPH, Benign Prostate Hyperplasia; PSA, Prostate-specific Antigen; PSA, prostate-specific antigen; SNP, signle nucleotide polymorphism; aOR (95%CI), age-adjusted odds ratio and respective 95% confidence interval. a Biopsy findings: normal, 14.9%; BPH, 5.4%, chronic prostatitis, 74.7%; atrophy, 5%; b Biopsy findings: normal, 73.5%; BPH, 26.5%; c Biopsy findings: normal, 22.2%; BPH, 6.0%, chronic prostatitis, 65.8%; atrophy, 6.0%.



Author Contributions

Conceived and designed the experiments: RJTR FMP CMSL RMM ELF. Performed the experiments: RJTR CPDM ASMA VFMC AMF FMP. Analyzed the data: RJTR AVR ELF. Contributed reagents/materials/analysis tools: RJTR CMSL RMM. Wrote the paper: RJTR ELF.


  1. 1. Gronberg H (2003) Prostate cancer epidemiology. Lancet 361: 859–864.
  2. 2. Andriole GL, Crawford ED, Grubb RL 3rd, Buys SS, Chia D, et al (2009) Mortality results from a randomized prostate-cancer screening trial. N Engl J Med 360: 1310–1319.
  3. 3. Schroder FH, Hugosson J, Roobol MJ, Tammela TL, Ciatto S, et al. (2009) Screening and prostate-cancer mortality in a randomized European study. N Engl J Med 360: 1320–1328.
  4. 4. Vickers AJ, Roobol MJ, Lilja H (2011) Screening for Prostate Cancer: Early Detection or Overdetection? Annu Rev Med.
  5. 5. Morote J, Trilla E, Esquena S, Serrallach F, Abascal JM, et al. (2002) The percentage of free prostatic-specific antigen is also useful in men with normal digital rectal examination and serum prostatic-specific antigen between 10.1 and 20 ng/ml. Eur Urol 42: 333–337.
  6. 6. Damber JE, Aus G (2008) Prostate cancer. Lancet 371: 1710–1721.
  7. 7. Lindstrom S, Schumacher F, Siddiq A, Travis RC, Campa D, et al. (2011) Characterizing associations and SNP-environment interactions for GWAS-identified prostate cancer risk markers–results from BPC3. PLoS One 6: e17142.
  8. 8. Febbo PG (2009) Genomic approaches to outcome prediction in prostate cancer. Cancer 115: 3046–3057.
  9. 9. Aly M, Wiklund F, Xu J, Isaacs WB, Eklund M, et al. (2011) Polygenic risk score improves prostate cancer risk prediction: results from the stockholm-1 cohort study. Eur Urol 60: 21–28.
  10. 10. Hsing AW, Sakoda LC, Chua S Jr (2007) Obesity, metabolic syndrome, and prostate cancer. Am J Clin Nutr 86: s843–857.
  11. 11. von Hafe P, Pina F, Perez A, Tavares M, Barros H (2004) Visceral fat accumulation as a risk factor for prostate cancer. Obes Res 12: 1930–1935.
  12. 12. Mistry T, Digby JE, Desai KM, Randeva HS (2007) Obesity and prostate cancer: a role for adipokines. Eur Urol 52: 46–53.
  13. 13. Ribeiro R, Lopes C, Medeiros R (2006) The link between obesity and prostate cancer: the leptin pathway and therapeutic perspectives. Prostate Cancer Prostatic Dis 9: 19–24.
  14. 14. Moore SC, Leitzmann MF, Albanes D, Weinstein SJ, Snyder K, et al. (2009) Adipokine genes and prostate cancer risk. Int J Cancer 124: 869–876.
  15. 15. Ribeiro R, Vasconcelos A, Costa S, Pinto D, Morais A, et al. (2004) Overexpressing leptin genetic polymorphism (−2548 G/A) is associated with susceptibility to prostate cancer and risk of advanced disease. Prostate 59: 268–274.
  16. 16. Kote-Jarai Z, Singh R, Durocher F, Easton D, Edwards SM, et al. (2003) Association between leptin receptor gene polymorphisms and early-onset prostate cancer. BJU Int 92: 109–112.
  17. 17. Wang MH, Helzlsouer KJ, Smith MW, Hoffman-Bolton JA, Clipp SL, et al. (2009) Association of IL10 and other immune response- and obesity-related genes with prostate cancer in CLUE II. Prostate 69: 874–885.
  18. 18. Silha JV, Krsek M, Sucharda P, Murphy LJ (2005) Angiogenic factors are elevated in overweight and obese individuals. Int J Obes (Lond) 29: 1308–1314.
  19. 19. Gomez-Ambrosi J, Catalan V, Ramirez B, Rodriguez A, Colina I, et al. (2007) Plasma osteopontin levels and expression in adipose tissue are increased in obesity. J Clin Endocrinol Metab 92: 3719–3727.
  20. 20. Kern PA, Ranganathan S, Li C, Wood L, Ranganathan G (2001) Adipose tissue tumor necrosis factor and interleukin-6 expression in human obesity and insulin resistance. Am J Physiol Endocrinol Metab 280: E745–751.
  21. 21. Park J, Euhus DM, Scherer PE (2011) Paracrine and Endocrine Effects of Adipose Tissue on Cancer Development and Progression. Endocr Rev.
  22. 22. Quinton ND, Lee AJ, Ross RJ, Eastell R, Blakemore AI (2001) A single nucleotide polymorphism (SNP) in the leptin receptor is associated with BMI, fat mass and leptin levels in postmenopausal Caucasian women. Hum Genet 108: 233–236.
  23. 23. Sun Q, Cornelis MC, Kraft P, Qi L, van Dam RM, et al. (2010) Genome-wide association study identifies polymorphisms in LEPR as determinants of plasma soluble leptin receptor levels. Hum Mol Genet 19: 1846–1855.
  24. 24. da Silva BA, Bjorbaek C, Uotani S, Flier JS (1998) Functional properties of leptin receptor isoforms containing the gln–>pro extracellular domain mutation of the fatty rat. Endocrinology 139: 3681–3690.
  25. 25. Chakraborty G, Jain S, Behera R, Ahmed M, Sharma P, et al. (2006) The multifaceted roles of osteopontin in cell signaling, tumor progression and angiogenesis. Curr Mol Med 6: 819–830.
  26. 26. Weber GF, Lett GS, Haubein NC (2010) Osteopontin is a marker for cancer aggressiveness and patient survival. Br J Cancer 103: 861–869.
  27. 27. Ding Z, Wu CJ, Chu GC, Xiao Y, Ho D, et al. (2011) SMAD4-dependent barrier constrains prostate cancer growth and metastatic progression. Nature 470: 269–273.
  28. 28. Hummelshoj T, Ryder LP, Madsen HO, Odum N, Svejgaard A (2006) A functional polymorphism in the Eta-1 promoter is associated with allele specific binding to the transcription factor Sp1 and elevated gene expression. Mol Immunol 43: 980–986.
  29. 29. Pollak MN, Schernhammer ES, Hankinson SE (2004) Insulin-like growth factors and neoplasia. Nat Rev Cancer 4: 505–518.
  30. 30. Chen W, Wang S, Tian T, Bai J, Hu Z, et al. (2009) Phenotypes and genotypes of insulin-like growth factor 1, IGF-binding protein-3 and cancer risk: evidence from 96 studies. Eur J Hum Genet 17: 1668–1675.
  31. 31. Li L, Huang X, Huo K (2010) IGFBP3 polymorphisms and risk of cancer: a meta-analysis. Mol Biol Rep 37: 127–140.
  32. 32. Deal C, Ma J, Wilkin F, Paquette J, Rozen F, et al. (2001) Novel promoter polymorphism in insulin-like growth factor-binding protein-3: correlation with serum levels and interaction with known regulators. J Clin Endocrinol Metab 86: 1274–1280.
  33. 33. de Alencar SA, Lopes JC (2010) A comprehensive in silico analysis of the functional and structural impact of SNPs in the IGF1R gene. J Biomed Biotechnol 2010: 715139.
  34. 34. Modrek B, Lee C (2002) A genomic view of alternative splicing. Nat Genet 30: 13–19.
  35. 35. Chen M, Manley JL (2009) Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches. Nat Rev Mol Cell Biol 10: 741–754.
  36. 36. Almind K, Inoue G, Pedersen O, Kahn CR (1996) A common amino acid polymorphism in insulin receptor substrate-1 causes impaired insulin signaling. Evidence from transfection studies. J Clin Invest 97: 2569–2575.
  37. 37. Fall K, Stark JR, Mucci LA, Chan J, Stampfer MJ, et al. (2008) No association between a polymorphic variant of the IRS-1 gene and prostate cancer risk. Prostate 68: 1416–1420.
  38. 38. Cronauer MV, Schulz WA, Seifert HH, Ackermann R, Burchardt M (2003) Fibroblast growth factors and their receptors in urological cancers: basic research and clinical implications. Eur Urol 43: 309–319.
  39. 39. Schulz S, Kohler K, Schagdarsurengin U, Greiser P, Birkenmeier G, et al. (2005) The human FGF2 level is influenced by genetic predisposition. Int J Cardiol 101: 265–271.
  40. 40. Culig Z, Steiner H, Bartsch G, Hobisch A (2005) Interleukin-6 regulation of prostate cancer cell growth. J Cell Biochem 95: 497–505.
  41. 41. Dossus L, Kaaks R, Canzian F, Albanes D, Berndt SI, et al. (2010) PTGS2 and IL6 genetic variation and risk of breast and prostate cancer: results from the Breast and Prostate Cancer Cohort Consortium (BPC3). Carcinogenesis 31: 455–461.
  42. 42. Tan D, Wu X, Hou M, Lee SO, Lou W, et al. (2005) Interleukin-6 polymorphism is associated with more aggressive prostate cancer. J Urol 174: 753–756.
  43. 43. Terry CF, Loukaci V, Green FR (2000) Cooperative influence of genetic polymorphisms on interleukin 6 transcriptional regulation. J Biol Chem 275: 18138–18144.
  44. 44. Mullberg J, Oberthur W, Lottspeich F, Mehl E, Dittrich E, et al. (1994) The soluble human IL-6 receptor. Mutational characterization of the proteolytic cleavage site. J Immunol 152: 4958–4968.
  45. 45. Santer FR, Malinowska K, Culig Z, Cavarretta IT (2010) Interleukin-6 trans-signalling differentially regulates proliferation, migration, adhesion and maspin expression in human prostate cancer cells. Endocr Relat Cancer 17: 241–253.
  46. 46. Danforth KN, Rodriguez C, Hayes RB, Sakoda LC, Huang WY, et al. (2008) TNF polymorphisms and prostate cancer risk. Prostate 68: 400–407.
  47. 47. Jacobs EJ, Hsing AW, Bain EB, Stevens VL, Wang Y, et al. (2008) Polymorphisms in angiogenesis-related genes and prostate cancer. Cancer Epidemiol Biomarkers Prev 17: 972–977.
  48. 48. Albertsen PC, Hanley JA, Fine J (2005) 20-year outcomes following conservative management of clinically localized prostate cancer. JAMA 293: 2095–2101.
  49. 49. Harnden P, Shelley MD, Coles B, Staffurth J, Mason MD (2007) Should the Gleason grading system for prostate cancer be modified to account for high-grade tertiary components? A systematic review and meta-analysis. Lancet Oncol 8: 411–419.
  50. 50. Heidenreich A, Bellmunt J, Bolla M, Joniau S, Mason M, et al. (2011) EAU guidelines on prostate cancer. Part 1: screening, diagnosis, and treatment of clinically localised disease. Eur Urol 59: 61–71.
  51. 51. DeLong ER, DeLong DM, Clarke-Pearson DL (1988) Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics 44: 837–845.
  52. 52. Pencina MJ, D'Agostino RB Sr, D'Agostino RB Jr, Vasan RS (2008) Evaluating the added predictive ability of a new marker: from area under the ROC curve to reclassification and beyond. Stat Med 27: 157–172; discussion 207–112.
  53. 53. Sundstrom J, Byberg L, Gedeborg R, Michaelsson K, Berglund L (2011) Useful tests of usefulness of new risk factors: Tools for assessing reclassification and discrimination. Scand J Public Health 39: 439–441.
  54. 54. Cavadas V, Osorio L, Sabell F, Teves F, Branco F, et al. (2010) Prostate cancer prevention trial and European randomized study of screening for prostate cancer risk calculators: a performance comparison in a contemporary screened cohort. Eur Urol 58: 551–558.