Despite great efforts to improve diagnosis and treatment, tuberculosis (TB) remains a major health problem worldwide, especially in developing countries. Lack of concrete immune markers is still the obstacle to properly evaluate active TB. Therefore, identification of more validated biomarkers and phenotypic signatures is imperative. In particular, T cell-related biomarkers are more significant.
To understand the nature of CD4+ T cell-derived signatures involved in infection and disease development, we examined and analyzed whole genome expression profiles of purified CD4+ T cells from healthy individuals (HD), two distinct populations with latent infection (with low or high IFN-γ levels, LTBL/LTBH) and untreated TB patients. Following, we validated the expression profiles of genes in the peripheral CD4+ T cells from each group and examined secretion levels of distinct cytokines in serum and pleural effusion.
Our bio-informatic analyses indicate that the two latent populations and clinical TB patients possess distinct CD4+ T cell gene expression profiles. Furthermore, The mRNA and protein expression levels of B cell activating factor (BAFF), which belongs to the TNF family, and a proliferation-inducing ligand (APRIL) were markedly up-regulated at the disease stage. In particular, the dramatic enhancement of BAFF and APRIL in the pleural effusion of patients with tuberculosis pleurisy suggests that these proteins may present disease status. In addition, we found that the BAFF/APRIL system was closely related to the Th1 immune response. Our study delineates previously unreported roles of BAFF and APRIL in the development of tuberculosis, and these findings have implications for the diagnosis of the disease. Our study also identifies a number of transcriptional signatures in CD4+ T cells that have the potential to be utilized as diagnostic and prognostic tools to combat the tuberculosis epidemic.
Citation: Liu K, Zhang Y, Hu S, Yu Y, Yang Q, Jin D, et al. (2012) Increased Levels of BAFF and APRIL Related to Human Active Pulmonary Tuberculosis. PLoS ONE 7(6): e38429. doi:10.1371/journal.pone.0038429
Editor: Francesco Dieli, University of Palermo, Italy
Received: April 3, 2012; Accepted: May 9, 2012; Published: June 12, 2012
Copyright: © 2012 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work is sponsored by National Eleven-fifth Mega-Scientific project on “prevention and treatment of AIDS, viral hepatitis and other infectious diseases” (2008ZX10003-012 & 2008ZX10003-005, 2012ZX10003-002), by China PR and by the National Natural Science Foundation of China (NSFC863367 & NSFC81000716). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Despite efforts to improve diagnosis and treatment, tuberculosis (TB) remains a profound burden on global health, especially in developing countries . Mycobacterium Tuberculosis (M.tb) infection can be controlled in most infected individuals. However, in a minority of cases (5∼10%), the failure to contain the infection induces active tuberculosis . Similar to the active disease, latent tuberculosis also includes a diverse range of patients –. This diversity makes the study of tuberculosis difficult, and more efficient tools are needed to further characterize the disease.
A well-used approach to the study of complex diseases is genome-wide transcriptional analysis. Previous studies have successfully obtained useful information regarding specific disease-associated signatures and the pathogenesis of tuberculosis using transcriptome analysis–. These studies have analyzed the gene expression profiles of cells involved in the immune response , , . Scanning of purified peripheral blood mononuclear cells (PBMCs) rather than whole blood is also a common method  because it allows the identification of a set of signatures for differentiating tuberculosis patients from healthy individuals (including latent tuberculosis patients) ,  and discriminating the subtle differences .
CD4+ T cell-mediated immune responses play pivotal roles in controlling the growth of M.tb and maintaining the homeostasis of the host . IFN-γ is a cytokine that strongly promotes the T helper 1 (Th1) cell response, and it has also been demonstrated to act as a protective factor against tuberculosis in human and animal studies . The IFN-γ release assay is widely used in helping to diagnose M.tb infection. However, the debate of its clinic use continues as IFN-γ release assay fails to distinguish between LTBI and active TB. In our study, participants with latent infection were segregated into two groups according to M.tb-specific IFN-γ spot-forming cells (SFCs), as determined by a well-used tool of enzyme-linked immunospot (ELISPOT) assay , . We discovered that two latent groups (low IFN-γ expression group with lower SFCs and high IFN-γ expression group with higher SFCs) possessed distinct gene expression profiles in their purified peripheral CD4+ T cells. Importantly, a subset of latent individuals (with high IFN-γ expression) had transcriptional signatures similar to those of active tuberculosis patients , In addition, we detected an increase in the expression of BAFF and APRIL during the disease stage, but not in latency population. The strong expression levels of BAFF and APRIL in the pleural effusion of patients with tuberculosis pleurisy (TP) further indicated their effects on disease status and in extrapulmonary TB. Moreover, soluble APRIL correlated with IFN-γ expression level in the pleural effusion of patients with TP, which suggests that the correlation between APRIL and IFN-γ may be useful for predicting tuberculosis.
Materials and Methods
Subjects and Samples
Healthy individuals with no history of tuberculosis disease, smear positive untreated pulmonary tuberculosis patients (TB, n = 39), and patients with tuberculosis pleurisy (TP, n = 21) were recruited from the Tuberculosis hospital, China. The tuberculin skin test (TST) and a previously established M.tb-specific IFN-γ ELISPOT assay  were employed to differentiate latent tuberculosis participants (LTB, n = 68) from healthy donors (HD, n = 50). Furthermore, the participants in the LTB group were segregated into two groups according to SFC counts: LTBL (n = 35, 30∼80 SFCs) and LTBH (n = 33, 110∼400 SFCs). Pulmonary cancer patients (CA, n = 14) were recruited as a disease control. Clinical diagnosis of pulmonary TB and TP was based on signs and symptoms, roentgenographic findings (Chest X-ray and/or HRCT) consistent with TB and sputum bacterium examination. All participants were HIV-negative, had no autoimmune diseases and were not subjected to immunosuppressant treatments. The subjects’ characteristics are summarized in Table 1.
PBMCs were isolated from heparinized whole blood, and CD4+ T cells were directly purified from PBMCs using magnetic beads (BD Biosciences). Total RNA was extracted from purified CD4+ T cells (CD4-RNA) and CD4+ T cell-depleted PBMCs (N-CD4-RNA). In addition, plasma and pleural effusions were also collected. This work was approval by and carried out under the guidelines of the Ethical Committee of the Shenzhen–Hong Kong Institute of Infectious Disease, Shenzhen Third People’s Hospital and written informed consent was obtained from all participants involved in this study directly and not from the next of kin or careers because all of them are adult.
CD4-RNA samples from identical groups (HD, n = 11; LTBL, n = 11; LTBH, n = 12; TB, n = 11) were equally mixed to create the RNA pools used for the microarray tests. The hybridization protocol for the human whole-genome oligonucleotide microarray (Agilent Technologies) and the bioinformatics analysis are shown in Supporting Information S1 (Microarray Test and Bioinformatics Analysis). The data were deposited in GEO at http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27882.
Real-Time PCR Validation
CD4-RNA was used to validate the 7 genes that were significantly up-regulated in patients with tuberculosis using real-time PCR (SYBR Green method). The N-CD4-RNA levels of BAFF and APRIL were also measured to detect their cellular distribution. The mRNA values were normalized to the housekeeping gene β-actin. Details of the real-time PCR protocol and primer sequences are available in the Online Supplement (Table S1).
Measurement of Soluble and Membrane-bound Proteins
The levels of soluble BAFF and APRIL were detected in the plasma, pleural effusion and supernatants of M.tb antigen-stimulated PBMCs using commercial ELISA kits (Bender MedSystems). Membrane-bound BAFF, BAFF receptor (BAFFR) and transmembrane activator and CAML interactor (TACI) on CD4+ T cells with/without M.tb antigen stimulation were detected by flow cytometry. The levels of Th1 cytokines (IFN-γ, IL-12p70 and IL-2) in the plasma and pleural effusions were determined using Luminex platform.
. Whole genome datasets of peripheral CD4+ T cells were analyzed by hierarchical clustering. The HD and LTBL groups clustered directly as a new category, which was subsequently clustered with the LTBH group. The TB group did not directly cluster with any group. B. In accordance with a 2-fold change between LTBL/LTBH/TB and HD, the number of significantly up-regulated (up arrow) and down-regulated (down arrow) genes were identified in the LTBL, LTBH and TB groups when they were compared to the HD group. Overlapping areas represent the common significant genes shared by two or three groups. C. The enrichment p value was used to rank the KEGG pathways (p value <0.05, included significant gene ≥10) in the LTBL, LTBH and TB groups. The p value of a KEGG pathway implies the significance of the KEGG pathway; the smaller the p value, the more significant the KEGG pathway. The TB group had the largest number of ranked KEGG pathways (36 pathways). The LTBL and LTBH groups had three and six ranked pathways, respectively.
Unpaired t-tests were used to analyze the difference between two groups. Pearson’s t-tests were employed to detect correlations between sAPRIL and the Th1 cytokines. Data are presented as the means ± SEM, and differences were considered significant if P value <0.05.
A. Compared with the HD group, the expression levels of the genes related to the TNFSF and TNFRSF in the LTBL, LTBH and TB groups were analyzed using a clustering algorithm. Eight genes (TRAIL, BAFF, CD30, APRIL, LTBR, TNFR1, TRAILR3 and CD40) were clustered as an identical category characterized by TB specific up-regulation. B. Using real-time PCR, we validated the eight TB-specific genes and detected seven genes that were significantly increased in the peripheral CD4+ T cells of TB patients. The mRNA values of the validated genes were normalized to the housekeeping gene β-actin. The numbers of participants included in validation test were the following: HD, n = 10; LTBL, n = 12; LTBH, n = 7; TB, n = 10. The validation results among the seven genes of the pentagram are marked BAFF and APRIL in Figure 3.A.
Genome-wide Analysis of CD4+ T Cell-related Genes
Genome-wide microarray analysis was employed to examine the gene expression profiles of peripheral CD4+ T cells. Whole microarray datasets of CD4+ T cells from HD, LTBL, LTBH and TB patients were analyzed using a clustering algorithm. The cluster analysis indicated that the two latent groups were not directly related, but the HD and LTBL groups clustered to form a new category, which then clustered with the LTBH group. The TB group did not cluster with any group directly (Figure 1.A). The genes that exhibited significant changes in expression (at least a 2-fold change between the HD group and other groups) were selected. Figure 1B shows the number of up- (up arrow) and down-regulated (down arrow) genes in the LTBL, LTBH and TB groups. Compared with the HD group, the TB group possessed the largest number of significantly altered genes, followed by the LTBH and LTBL groups (Figure 1.B).
A. BAFF and APRIL mRNA were detected in both peripheral CD4+ T cells (CD4-RNA; HD, n = 10; LTBL, n = 12; LTBH, n = 7; TB, n = 10) and CD4+ T cell-depleted PBMCs (N-CD4-RNA; HD, n = 6; LTBL, n = 12; LTBH, n = 7; TB, n = 6). The gene expression levels of BAFF and APRIL were normalized to β-actin. B. Soluble BAFF (sBAFF) and APRIL (sAPRIL) were measured by ELISA in plasma (HD, n = 22; LTBL, n = 12; LTBH, n = 14; TB, n = 11; TP, n = 11; CA, n = 14) and pleural effusion (TP, n = 10; CA, n = 14). C. Flow cytometry was employed to detect BAFF, BAFFR and TACI expression on the surface of peripheral CD4+ T cells from HD participants (n = 2) and TB patients (n = 2). D. M.tb antigen stimulation slightly augmented the frequency of BAFFR+CD4+ T cells in PBMCs from TB patients (TB-sti, n = 2) compared with un-stimulated cells (TB n-sti, n = 2). E. With or without M.tb antigen stimulation, the levels of sBAFF and sAPRIL were detected by ELISA in the supernatants of PBMCs from TB patients (n = 5). The stimulated supernatants of PBMCs from the HD group (n = 5) were used as a control. HD-sti: M.tb antigen-stimulated PBMCs from HD participants; TB-sti: M.tb antigen-stimulated PBMCs from TB patients; TB n-sti: un-stimulated PBMCs from TB patients.
To further elucidate biological changes in the peripheral CD4+ T cells from the LTBL, LTBH and TB groups, enrichment p values were calculated and used to rank the GO terms (p<0.001, included significant genes ≥20) and KEGG pathways (p value <0.05, included significant genes ≥10) for each group. The p value for each individual GO term/KEGG pathway indicates whether a term or a pathway is significant between analyzed two groups; the smaller p value indicates the more significance of GO term/KEGG pathway . Thus, we can speculate the extent of the effects of certain genes on CD4+ T cells according to their GO term/KEGG pathway rankings. The LTBL group had 17 ranked GO terms, which were involved in responses to stimuli, immune responses and cell-cell signaling. The LTBH and TB groups had 30 and 34 ranked GO terms, respectively. Beside the GO terms ranked in the LTBL analysis, significantly altered genes identified in the LTBH, LTBL, and TB groups were related to protein/RNA metabolism and protein/nucleic acid modification (Spread Sheet S1). With regard to ranked KEGG pathways (Figure 1.C), the significantly altered genes in the LTBL group were annotated to include 3 ranked KEGG pathways that were mainly involved in ligand-receptor interactions and antigen processing and presentation. The LTBH group contained 6 ranked KEGG Pathways, which were primarily associated with antigen processing and presentation, cell adhesion, extracellular matrix (ECM)-receptor interaction, autophagy regulation and p53 signaling. The significantly altered genes in the TB group were annotated to include 36 ranked KEGG pathways. In addition to the shared pathways of the LTBL and LTBH groups, the TB group-specific ranked KEGG pathways were also associated with the regulation of cellular events, such as signal transduction (in particular the MAPK signaling pathway), cytoskeletal rearrangement, adhesion, apoptosis, autophagy, and metabolism (Spread Sheet S2). These results suggest that there may be no major differences in CD4+ T cell function between TB and LTBH patients.
Ingenuity Pathway Analysis (IPA) was used to identify the forty-five BAFF- and/or APRIL-related genes represented on the microarray. BAFF and APRIL (yellow marked genes) were found to interact with many genes that are involved in regulating apoptosis, transcription, the immune response and signal transduction. Among these genes, the T helper 1 (Th1)-related genes, such as IFN-γ, IL-2, IL-27 and TNF (namely, TNF-α), were identified to interact with BAFF and/or APRIL. These genes are presented by their common abbreviated names. According to their direct (solid lines) or indirect (dashed lines) action, these genes can establish contacts with BAFF and/or APRIL. Lines ending in black arrows indicate an activation pathway, and those ending in white arrows represent a translocation process. Lines joining two genes indicate binding.
Validation of TNFSF- and TNFRSF-Associated Genes
Previous studies have reported that the TNFSF and TNFRSF genes played roles in disease of tuberculosis , . Furthermore, we noticed that tested genes belonging to the TNF and TNF receptor superfamilies (TNFSF and TNFRSF, respectively) exhibited a significant (greater than 2-fold) change in expression levels in the array test (Table S2). Figure 2.A shows that eight genes in the TNF and TNFR superfamilies were markedly up-regulated in the TB group. Therefore, we validated the expression levels of these genes in purified peripheral CD4+ T cells of each group (HD, n = 10; LTBL, n = 12; LTBH, n = 7; TB, n = 10), which samples were not the same for genome array assay. Except for the expression levels of TNFR1, the expression levels of the seven other genes were confirmed by real-time PCR to be significantly increased in TB patients (Figure 2.B). The validation of BAFF and APRIL will be shown in the following section.
. The levels of IFN-γ, IL-12p70 and IL-2 were detected in plasma (HD, n = 15; LTBL, n = 11; LTBH, n = 14; TB, n = 11) and pleural effusion (TP, n = 10) using Luminex technology. B. Significant positive correlations were identified between sAPRIL, and IFN-γ (Pearson’s correlation coefficient, r = 0.8412, p = 0.0023), IL-12p70 (Pearson’s correlation coefficient r = 0.8211, p = 0.0036) and IL-2 (Pearson’s correlation coefficient r = 0.7424, p = 0.0139) in the TP pleural effusion.
Detection of the BAFF/APRIL System
Among the seven up-regulated genes in the group of TB, we further studied BAFF and APRIL in detail. BAFF and APRIL are ligands of the BAFF/APRIL system and expressed on many cells . To examine the cellular distribution of BAFF and APRIL expression, we further analyzed the mRNA expression levels of these genes in peripheral CD4+ T cells and CD4+ T cell-depleted PBMCs. BAFF mRNA was significantly increased in the CD4+ T cells of TB patients (TB vs. HD, p = 0.0119). An examination of the N-CD4-RNA samples revealed that BAFF mRNA was significantly increased in both LTB participants (LTBL vs. HD, p = 0.0049; LTBH vs. HD, p = 0.0027) and TB patients (TB vs. HD, p = 0.0007). The significant increase in APRIL mRNA was only encountered in the CD4+ T cells of TB patients (TB vs. HD, p = 0.0112), and no significant up- or down-regulation could be detected in other groups (Figure 3.A). mRNA of the membrane-bound APRIL  ligand TWE-PRIL was not detected in the CD4-RNA or N-CD4-RNA samples (data not shown).
BAFF and APRIL can be secreted and act as soluble regulators . Indeed, ELISA tests demonstrated that soluble BAFF (sBAFF) and soluble APRIL (sAPRIL) were significantly elevated in the plasma of TB and TP patients (Figure 3.B, TB, n = 12 vs. HD, n = 22: both sBAFF and sAPRIL, p<0.0001; TP, n = 10 vs. HD: sBAFF, p = 0.0003 and sAPRIL, p<0.0001). The plasma levels of sBAFF and sAPRIL were not significantly different between TB and TP patients. However, sBAFF protein levels were significantly elevated in the plasma of TB and TP patients (both were p<0.0001) compared with that of CA patients (n = 14), whereas sAPRIL levels were similar among TB, TP and CA participants. Both BAFF and APRIL showed the highest expression in the pleural effusion of TP patients, and the levels of these proteins were significantly higher than those in the pleural effusion of CA individuals. In summary, Figure 3.B shows the tuberculosis-specific up-regulation of BAFF and APRIL. In particular, the high expression of BAFF and APRIL in the TP pleural effusion was remarkable.
BAFFR and TACI are the key receptors of the BAFF/APRIL system . An analysis by flow cytometry revealed that the frequencies of the CD4+BAFF+ (from 0.20% to 2.16%), CD4+BAFFR+ (from 0.26% to 0.37%) and CD4+TACI+ (from 1.36% to 5.10%) T cells were slightly increased in the PBMCs of TB patients (n = 2) compared with HD participants (n = 2) (Figure 3.C). After M.tb-specific antigen stimulation, the frequency of CD4+BAFFR+ T cells was enhanced in the PBMCs of TB patients (n = 2) compared with un-stimulated cells (from 0.51% to 1.13%, Figure 3.D). Interestingly, there was less sAPRIL present in the supernatants of M.tb-stimulated PBMCs (TB-sti) from TB patients (n = 5) than in un-stimulated PBMCs (TB n-sti) (p = 0.0414); furthermore, sAPRIL was not detected in the PBMC supernatants from HD patients (HD-sti, n = 5) upon M.tb antigen stimulation. The supernatant levels of sBAFF were not significantly different between stimulated and un-stimulated cells (Figure 3.E).
Ingenuity Pathways Analysis For BAFF and/or APRIL-Related Genes
Ingenuity Pathways Analysis (IPA) is a common method to depict known interactions among genes , . To further decipher possible roles of BAFF and APRIL in disease of Tuberculosis, IPA was employed to understand the BAFF- and/or APRIL-related genes represented in the microarray (Figure 4). Figure 4 shows that there are forty-five BAFF- and/or APRIL-related genes interacted, most of them are involved in regulating apoptosis, immune responses and signal transduction.
Th1 cytokines (IFN-γ, IL-2, IL-27 and TNF/TNF-α) were also identified by IPA, which suggests that there is an intimate connection between the Th1 response and the BAFF/ARPIL system. Thus, we examined the levels of Th1 cytokines (IFN-γ, IL-12p70 and IL-2) in plasma (HD, n = 15; LTBL, n = 11; LTBH, n = 14; TB, n = 11) and pleural effusion from TP patients (n = 10) by Luminex platform. IFN-γ and IL-12p20 were slightly elevated in the pleural effusion of TP patients compared with the plasma of the other groups (Figure 5.A). In the pleural effusion of TP patients, positive correlations were identified between sAPRIL and IFN-γ (Pearson’s correlation coefficient: r = 0.8412, p = 0.0023), IL-12p70 (Pearson’s correlation coefficient: r = 0.8211, p = 0.0036) and IL-2 (Pearson’s correlation coefficient: r = 0.7424, p = 0.0139) (Figure 5.B). No correlation was found between sBAFF and Th1 cytokines in TP pleural effusion.
In the current study, we showed that the two latent TB groups (LTBL, LTBH) did not cluster closely. Among detected genes, LTBL and HD clustered as a category, but LTBH is more close to active Tuberculosis. These results suggest that LTBL and HD participants have similar gene expression profiles in their peripheral CD4+ T cells. Although LTBL and LTBH are both latent TB classes, their transcription profiles are actually quite distinct. The TB group did not cluster with any other group directly, which indicates that the peripheral CD4+ T cells of these patients have unique gene expression profiles, and these characteristics help us to well understand TB patients from healthy and latent participants.
With regard to ranked GO terms or KEGG Pathways, the significantly altered expression of genes in the LTBL group were mainly associated with stimulus responses, suggesting that the CD4+ T cells of the LTBL participants might easily respond to M.tb-antigen stimulation. The CD4+ T cells of the LTBH group could easily alternate between their roles in movement and metabolism because many migration- and metabolism-related GO terms were ranked in the LTBH group. The TB group has the largest number of genes ranked by GO terms and KEGG pathways. This is reasonable to assume that the CD4+ T cells of TB patients do not appear to be functionally impaired and may possess the most protective potential and act for alterations in metabolism, migration, adhesion, apoptosis and respond to stimulus.
Although many studies have confirmed the indispensability of IFN-γ in the prevention of TB development, correlation of IFN-γ levels and protection ability still debate . Therefore, it is essential to identify other tuberculosis-specific signatures. In this study, TNFSF and TNFRSF genes were screened for further validation because some of them have previously been confirmed to have effects on TB development. TNF-α promotes granuloma formation, which is involved in preventing the dissemination of bacilli , and anti-TNF-α treatment for rheumatoid arthritis increases the risk of active tuberculosis development . FAS, FASLG and CD30 have also been shown to be related to disease development , , . In our study, soluble CD30 and FASLG were also markedly up-regulated in the plasma of tuberculosis patients (data not shown).
Furthermore, our results indicate that BAFF and APRIL expression is significantly elevated during active tuberculosis infections. And, a stronger secretion of soluble BAFF and APRIL was observed in the pleural effusion of TP patients, which suggests that the two cytokines are involved in pulmonary and extraplumonary Tuberculosis and may reflect disease exacerbation. Although the levels of sAPRIL were lower in the M.tb antigen-stimulated supernatants than in un-stimulated supernatants of TB patients, we hypothesize that the down-regulation of sAPRIL is caused by its consumption during stimulation because it has been shown to play an anti-apoptotic role through an autocrine pathway , .
BAFF and APRIL previously reported as two important mediators of B cells , also affect the survival and activation of T cells –. In particular, BAFF also promotes the Th1 response . Certain cytokines, such as IFN-γ, IL-10 and IFN-α, can induce various cells to augment BAFF production , . However, IL-4 can inhibit BAFF expression . Similar to BAFF, APRIL has been shown to be up-regulated in response to IFN-γ and IFN-α treatment , . In our study, TB and TP patients secreted high antigen specific IFN-γ level detected by ELISPOT (SFCs>110), and plasma level of IFN-γ were markedly increased in the pleural effusion of TP patients but with low IL-4 level (Figure S1). Therefore, the dramatic enhancement of sBAFF and sAPRIL levels in patients might be caused by the stronger IFN-γ-mediated effects that outweigh the inhibitory effects of IL-4. The exact mechanisms of sBAFF and sAPRIL production require further examination. BAFF has two forms: membrane-bound and soluble . An analysis by flow cytometry revealed that the frequency of BAFF+CD4+ T cells is increased in TB patients (Figure 3.C). Some studies have suggested that only immobilized BAFF can enhance the human T cell response to anti-CD3/TCR activation –. Thus, it appears that sBAFF might act as a suppressor and inhibit the binding of membrane-bound BAFF to its receptors.
Figure 4 shows there are forty-five known genes that interact with BAFF and/or APRIL. An IPA analysis was employed to analyze the interactions and RT-PCR results indicated that a largest number of pathways emanate from apoptosis-related genes, such as BCL2 (data not shown). Therefore, network-regulated apoptosis is important for the fate of CD4+ T cells and might indirectly affect tuberculosis outcome. As an autocrine factor , , , BAFF has also been reported to induce BCL2 up-regulation through BAFFR-mediated signaling  and to promote T cell survival . The cytoplasmic tail of BAFFR has TNF receptor-associated factor (TRAF)-interacting motifs (TIMs) , which are involved in mediating cell survival, activation and differentiation. Thus, BAFF might prevent peripheral CD4+ T cells from apoptosis in patients and this mechanism might be related to the induction of BCL2 gene expression.
Figure 4 also shows the intimate association between the Th1 response and the BAFF/APRIL system. The Th1 response plays an indispensable role in M.tb control and elimination –. In particular, sufficient macrophage activation is produced by IFN-γ and IL-12, thus permitting more efficient bacilli destruction . Deficiencies in IL-12 or IFN-γ or their receptors render individuals more susceptible to mycobacterial infections , . TNF-α is important for establishing granulomas and the localized control of M.tb . A few publications revealed that BAFF is expressed on activated T cells and promotes the Th1 response , . BAFF and IFN-γ have also been shown to establish an inflammatory loop between T and myeloid cells that exacerbates autoimmunity . To our knowledge, no direct relation between APRIL and the Th1 response has been reported. However, among the TNFSF-related genes, APRIL is most closely related to BAFF; they share ∼30% sequence identity in their TNF homology domains (THDs) . APRIL may play a similar role in disease of Tuberculosis. Moreover, we found strong positive correlations between Th1 cytokines (IFN-γ, IL-12p70 and IL-2) and sAPRIL in the pleural effusion of TP patients. Thus, these studies suggest a reciprocal relationship between the BAFF/APRIL system and the Th1 response in pulmonary and extrapulmonary TB patients. In our study, we found effects of the BAFF/APRIL system on tuberculosis to be intriguing. Future studies involving novel ideas and participants are necessary to discern the role of the BAFF/APRIL system in the pathogenesis of tuberculosis.
In summary, our results herein suggest that the elevated expression levels of BAFF and APRIL are associated with active pulmonary and extrapulmonary tuberculosis and that the BAFF/APRIL system is intimately correlated with the Th1 response. These results implicate a potential use for the combination of BAFF and/or APRIL with IFN-γ in the diagnosis of tuberculosis. Moreover, the CD4+ T cell-derived transcriptional signatures of distinct latency populations might be helpful to identify subsets of latent individuals that produce protective responses against tuberculosis. However, longitudinal studies must be performed to assess this hypothesis.
The Levels of IFN-γ and IL-4 in Pleural Effusion from TP (n = 10) and CA (n = 5) patients. Secretion levels of IFN-γ and IL-4 were detected in pleural effusion from TP (tuberculosis pleural, n = 10) and CA (lung cancer, n = 5) patients by Luminex.
An excel spread sheet listing the ranked GO terms in LTBL, LTBH and TB.
An excel spread sheet listing the ranked KEGG pathways in LTBL, LTBH and TB.
Microarray Test and Bioinformatics Analysis.
Target Genes and Primer Sequences. Primer pairs of selected genes shown in Table S1. *β-actin was a housekeeping gene.
The Expression Level of TNFSF (A) and TNFRSF (B) family genes in Microarray Test. Definition of abbreviations: HD = healthy donors; LTBL = latent tuberculosis participants with low SFCs; LTBH = latent tuberculosis participants with high SFCs; TB = pulmonary tuberculosis patients. ID REF is the ProbeName on microarray. The expression level was displayed as ratio between every two groups. The fold change ≥2 or ≤0.5 between two groups was set as cutoff to select significant genes. Gray bar indicated the existence of significant genes.
We thank Dr. Xinchun Chen, Mingfeng Liao, Xiuyun Zhu and Weilong Liu (Shenzhen Third People’s Hospital, China) for their assistance in recruiting samples for this paper.
Conceived and designed the experiments: HYL QJ. Performed the experiments: KL YZ SZH YY DDJ QTY. Analyzed the data: HYL QJ. Contributed reagents/materials/analysis tools: XCC. Wrote the paper: HYL KL. Clinical sample recruitment: XCC QTY.
- 1. Barry CE 3rd, Boshoff HI, Dartois V, Dick T, Ehrt S, et al. (2009) The spectrum of latent tuberculosis: rethinking the biology and intervention strategies. Nat Rev Microbiol 7: 845–855.
- 2. Kaufmann SH, McMichael AJ (2005) Annulling a dangerous liaison: vaccination strategies against AIDS and tuberculosis. Nat Med 11: S33–44.
- 3. Young DB, Gideon HP, Wilkinson RJ (2009) Eliminating latent tuberculosis. Trends Microbiol 17: 183–188.
- 4. Maertzdorf J, Repsilber D, Parida SK, Stanley K, Roberts T, et al. (2011) Human gene expression profiles of susceptibility and resistance in tuberculosis. Genes Immun 12: 15–22.
- 5. Berry MP, Graham CM, McNab FW, Xu Z, Bloch SA, et al. (2010) An interferon-inducible neutrophil-driven blood transcriptional signature in human tuberculosis. Nature 466: 973–977.
- 6. Jacobsen M, Repsilber D, Gutschmidt A, Neher A, Feldmann K, et al. (2007) Candidate biomarkers for discrimination between infection and disease caused by Mycobacterium tuberculosis. J Mol Med (Berl) 85: 613–621.
- 7. Homolka S, Niemann S, Russell DG, Rohde KH (2010) Functional genetic diversity among Mycobacterium tuberculosis complex clinical isolates: delineation of conserved core and lineage-specific transcriptomes during intracellular survival. PLoS Pathog 6: e1000988.
- 8. Tailleux L, Waddell SJ, Pelizzola M, Mortellaro A, Withers M, et al. (2008) Probing host pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages. PLoS One 3: e1403.
- 9. Querec TD, Akondy RS, Lee EK, Cao W, Nakaya HI, et al. (2009) Systems biology approach predicts immunogenicity of the yellow fever vaccine in humans. Nat Immunol 10: 116–125.
- 10. Flynn JL, Chan J (2001) Immunology of tuberculosis. Annu Rev Immunol 19: 93–129.
- 11. Chen X, Yang Q, Zhang M, Graner M, Zhu X, et al. (2009) Diagnosis of active tuberculosis in China using an in-house gamma interferon enzyme-linked immunospot assay. Clin Vaccine Immunol 16: 879–884.
- 12. Chen X, Zhang M, Liao M, Graner MW, Wu C, et al. (2010) Reduced Th17 response in patients with tuberculosis correlates with IL-6R expression on CD4+ T Cells. Am J Respir Crit Care Med 181: 734–742.
- 13. Reiner A, Yekutieli D, Benjamini Y (2003) Identifying differentially expressed genes using false discovery rate controlling procedures. Bioinformatics 19: 368–375.
- 14. Budak F, Uzaslan EK, Cangur S, Goral G, Oral HB (2008) Increased pleural soluble fas ligand (sFasL) levels in tuberculosis pleurisy and its relation with T-helper type 1 cytokines. Lung 186: 337–343.
- 15. Ng LG, Mackay CR, Mackay F (2005) The BAFF/APRIL system: life beyond B lymphocytes. Mol Immunol 42: 763–772.
- 16. Daridon C, Youinou P, Pers JO (2008) BAFF, APRIL, TWE-PRIL: who's who? Autoimmun Rev 7: 267–271.
- 17. Mackay F, Schneider P, Rennert P, Browning J (2003) BAFF AND APRIL: a tutorial on B cell survival. Annu Rev Immunol 21: 231–264.
- 18. Crouser ED, Culver DA, Knox KS, Julian MW, Shao G, et al. (2009) Gene expression profiling identifies MMP-12 and ADAMDEC1 as potential pathogenic mediators of pulmonary sarcoidosis. Am J Respir Crit Care Med 179: 929–938.
- 19. Wang IM, Stepaniants S, Boie Y, Mortimer JR, Kennedy B, et al. (2008) Gene expression profiling in patients with chronic obstructive pulmonary disease and lung cancer. Am J Respir Crit Care Med 177: 402–411.
- 20. Kaufmann SH (2001) How can immunology contribute to the control of tuberculosis? Nat Rev Immunol 1: 20–30.
- 21. Keane J, Gershon S, Wise RP, Mirabile-Levens E, Kasznica J, et al. (2001) Tuberculosis associated with infliximab, a tumor necrosis factor alpha-neutralizing agent. N Engl J Med 345: 1098–1104.
- 22. Hanekom WA, Hussey GD, Hughes EJ, Potgieter S, Yogev R, et al. (1999) Plasma-soluble CD30 in childhood tuberculosis: effects of disease severity, nutritional status, and vitamin A therapy. Clin Diagn Lab Immunol 6: 204–208.
- 23. Oddo M, Renno T, Attinger A, Bakker T, MacDonald HR, et al. (1998) Fas ligand-induced apoptosis of infected human macrophages reduces the viability of intracellular Mycobacterium tuberculosis. J Immunol 160: 5448–5454.
- 24. Kern C, Cornuel JF, Billard C, Tang R, Rouillard D, et al. (2004) Involvement of BAFF and APRIL in the resistance to apoptosis of B-CLL through an autocrine pathway. Blood 103: 679–688.
- 25. Chu VT, Enghard P, Schurer S, Steinhauser G, Rudolph B, et al. (2009) Systemic activation of the immune system induces aberrant BAFF and APRIL expression in B cells in patients with systemic lupus erythematosus. Arthritis Rheum 60: 2083–2093.
- 26. Sutherland AP, Ng LG, Fletcher CA, Shum B, Newton RA, et al. (2005) BAFF augments certain Th1-associated inflammatory responses. J Immunol 174: 5537–5544.
- 27. Ng LG, Sutherland AP, Newton R, Qian F, Cachero TG, et al. (2004) B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells. J Immunol 173: 807–817.
- 28. Huard B, Schneider P, Mauri D, Tschopp J, French LE (2001) T cell costimulation by the TNF ligand BAFF. J Immunol 167: 6225–6231.
- 29. Scapini P, Hu Y, Chu CL, Migone TS, Defranco AL, et al. (2010) Myeloid cells, BAFF, and IFN-gamma establish an inflammatory loop that exacerbates autoimmunity in Lyn-deficient mice. J Exp Med 207: 1757–1773.
- 30. Litinskiy MB, Nardelli B, Hilbert DM, He B, Schaffer A, et al. (2002) DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL. Nat Immunol 3: 822–829.
- 31. Nardelli B, Belvedere O, Roschke V, Moore PA, Olsen HS, et al. (2001) Synthesis and release of B-lymphocyte stimulator from myeloid cells. Blood 97: 198–204.
- 32. Craxton A, Magaletti D, Ryan EJ, Clark EA (2003) Macrophage- and dendritic cell–dependent regulation of human B-cell proliferation requires the TNF family ligand BAFF. Blood 101: 4464–4471.
- 33. Liu Y, Xu L, Opalka N, Kappler J, Shu HB, et al. (2002) Crystal structure of sTALL-1 reveals a virus-like assembly of TNF family ligands. Cell 108: 383–394.
- 34. Huard B, Arlettaz L, Ambrose C, Kindler V, Mauri D, et al. (2004) BAFF production by antigen-presenting cells provides T cell co-stimulation. Int Immunol 16: 467–475.
- 35. Rahman ZS, Manser T (2004) B cells expressing Bcl-2 and a signaling-impaired BAFF-specific receptor fail to mature and are deficient in the formation of lymphoid follicles and germinal centers. J Immunol 173: 6179–6188.
- 36. Dempsey PW, Doyle SE, He JQ, Cheng G (2003) The signaling adaptors and pathways activated by TNF superfamily. Cytokine Growth Factor Rev 14: 193–209.
- 37. Cooper AM, Dalton DK, Stewart TA, Griffin JP, Russell DG, et al. (1993) Disseminated tuberculosis in interferon gamma gene-disrupted mice. J Exp Med 178: 2243–2247.
- 38. Flynn JL, Chan J, Triebold KJ, Dalton DK, Stewart TA, et al. (1993) An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection. J Exp Med 178: 2249–2254.
- 39. Dalton DK, Pitts-Meek S, Keshav S, Figari IS, Bradley A, et al. (1993) Multiple defects of immune cell function in mice with disrupted interferon-gamma genes. Science 259: 1739–1742.
- 40. Alcais A, Fieschi C, Abel L, Casanova JL (2005) Tuberculosis in children and adults: two distinct genetic diseases. J Exp Med 202: 1617–1621.
- 41. Jouanguy E, Doffinger R, Dupuis S, Pallier A, Altare F, et al. (1999) IL-12 and IFN-gamma in host defense against mycobacteria and salmonella in mice and men. Curr Opin Immunol 11: 346–351.
- 42. Dillon SR, Gross JA, Ansell SM, Novak AJ (2006) An APRIL to remember: novel TNF ligands as therapeutic targets. Nat Rev Drug Discov 5: 235–246.