C1A cysteine peptidases are synthesized as inactive proenzymes. Activation takes place by proteolysis cleaving off the inhibitory propeptide. The inhibitory capacity of propeptides from barley cathepsin L and B-like peptidases towards commercial and barley cathepsins has been characterized. Differences in selectivity have been found for propeptides from L-cathepsins against their cognate and non cognate enzymes. Besides, the propeptide from barley cathepsin B was not able to inhibit bovine cathepsin B. Modelling of their three-dimensional structures suggests that most propeptide inhibitory properties can be explained from the interaction between the propeptide and the mature cathepsin structures. Their potential use as biotechnological tools is discussed.
Citation: Cambra I, Hernández D, Diaz I, Martinez M (2012) Structural Basis for Specificity of Propeptide-Enzyme Interaction in Barley C1A Cysteine Peptidases. PLoS ONE 7(5): e37234. https://doi.org/10.1371/journal.pone.0037234
Editor: Guy Smagghe, Ghent University, Belgium
Received: October 7, 2011; Accepted: April 18, 2012; Published: May 17, 2012
Copyright: © 2012 Cambra et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: Financial support from the Ministerio de Economia y Competitividad (project AGL2011-23650) and from the Universidad Politécnica de Madrid/Comunidad de Madrid (project CCG10-UPM/AGR-5242) is gratefully acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Plant proteolysis is a complex process that involves broad metabolic networks, different sub-cellular compartments and various types of peptidases, mainly cysteine-, serine-, aspartic- and metallo-peptidases . Among the about 800 peptidases encoded by plant genomes, approximately 140 correspond to cysteine-peptidases that belong to 15 families distributed in 5 clans . In particular, the papain-peptidases C1A (family C1, clan CA), grouped as cathepsin L-, B-, H- and F-like according to their gene structures and phylogenetic relationship , are the most abundant. Members of the papain-like subfamily C1A are the most widely studied among plant cysteine peptidases. All C1A proteins contain several disulphide bonds and share three conserved catalytic residues, Cys, His and Asn, in the catalytic triad and a Gln residue involved in maintaining an active enzyme conformation. C1A peptidases from plants are synthesized as inactive or little active precursors to prevent inappropriate proteolysis. Immature proteins comprise an N-terminal propeptide of 130–160 amino acids and the mature protein domain that is about 220–270 residues long. Three main functions have been assigned to C1A propeptides: inhibition of their cognate enzyme, participation in correct intracellular targeting of the protease, and assistance in folding of the mature enzyme . In this way, the pro-sequences play important roles as modulators of the peptidase activity to guarantee that the mature enzyme is formed in the right place and/or at the right time .
From crystal structures of procathepsins B and L from mammals , , it has been determined that the propeptide forms a predominantly α-helical domain, which is positioned at the top of the cysteine peptidase catalytic site, where it interacts with the mature part. The propeptide chain then continues in an extended conformation across the active-site cleft and towards the N-terminus of the mature enzyme in the reverse orientation to that of substrate binding. The C1A propeptides contain the consensus motif GxNxFxD, which seems to be essential for the correct processing of the peptidase precursors as well as the non-contiguous ERFNIN signature (Ex3Rx3Fx3Nx3I/Vx3N) found in cathepsin L- and H-like or the ERFNAQ variant in cathepsin F-like, both of unknown function , . In contrast, cathepsin B-like peptidases lack this motif , .
To become active, the C1A peptidases need to be processed either autocatalytically or with the aid of processing enzymes . Activation takes place by limited intra and intermolecular proteolysis cleaving off the inhibitory propeptide . For most C1A cysteine peptidases, activation mechanism has been proposed to be a two steps process , . One step corresponds to the enhancement of the accessibility to the scissile bond triggered by low pH through intramolecular conformational changes of the propeptide. The second step corresponds to the intermolecular proteolysis of the scissile bond achieved in an autocatalytic manner of by other proteases.
Selectivity of propeptide inhibition is a crucial feature to be addressed for using propeptides as biotechnological tools. In this way, many mammalian C1A propeptides not only are able to inhibit their cognate enzymes, but have the capacity to inhibit in trans several but not all related peptidases . In plants, only the inhibitory capacity of propeptides from papain and papaya proteinase IV against papaya cathepsin L-like cysteine peptidases has been determined , . In this paper, we characterize the inhibitory capacity of propeptides from barley cathepsin L and B-like peptidases towards commercial and barley cathepsins. Modelling of the three-dimensional structures suggests that most inhibitory properties can be explained from the interaction between the propeptide and mature cathepsin structures.
Purification of propeptides and inhibitory assays
To fully characterize the inhibitory properties of C1A propeptides in barley, we selected four cathepsin L-like (HvPap-4, -6, -10, -16) peptidases and one cathepsin B-like (HvPap-19) that had been previously described , . To study their inhibitory capability, their propeptides were purified as recombinant proteins from E. coli cultures. Bands detected after SDS-PAGE were in accordance with the expected molecular weights of purified propeptides, which rank from 12 kD of HvPap-19pro to 19 kD of HvPap-16pro (Fig. 1a). In vitro inhibitory assays against purified barley cathepsin L-like peptidases or commercial bovine cathepsin B were done using substrates able to be degraded by cathepsin L and B-like enzymes. The inhibitory activity of propeptides was not assayed against the barley cathepsin B-like HvPap-19 due to the difficulties of obtaining active purified forms from recombinant E. coli cultures. Kinetic analyses revealed that barley propeptides exhibited a competitive tight binding inhibition against all peptidases tested (Fig. 1b). The inhibition constant values (Ki) against the C1A cysteine peptidases were determined and showed different target specificities (Table 1). Overall, the lowest Ki data that reflects the highest inhibitory capacity of the propeptides were obtained for HvPap-4pro and HvPap-6pro, which were able to inhibit all barley L-like cathepsins tested. Interestingly, both propeptides inhibit better the peptidase activity of HvPap-10 than the activities of their cognate peptidases. Likewise, HvPap-10pro and HvPap-16pro inhibit worse their maternal peptidases than HvPap-4pro and HvPap-6pro, being the inhibitory capacity of HvPap-16pro specific of its cognate peptidase. On the other hand, the propeptide of the cathepsin B-like peptidase (HvPap-19pro) was able to inhibit the activity of the cathepsin L-like peptidases HvPap-4 and HvPap-16. However, it did not inhibit the activity of the bovine cathepsin B, which was also not inhibited by any of the cathepsin L-like propeptides tested.
A. Purification of the recombinant HvPap propeptides from E. coli cultures by SDS-PAGE. Five µg of each protein were loaded. Five µg of an extract of pRSETB without insert were used as a control of protein purity. The gel was stained with Coomassie Brillant Blue G. M: molecular markers (KD). B. Example of Lineweaver-Burk plot. HvPap-10 competitive inhibition in the presence of HvPap-4pro. Water was used as a control.
Structural explanation for bovine cathepsin B inhibition
To explain the lack of inhibition of HvPap-19 propeptide on bovine cathepsin B activity, we modelled the structure of both proteins using the crystallographic structure of the human procathepsin B as a template. Bovine cathepsin B and barley cathepsin B HvPap-19 aligned to human procathepsin B at sequence identities of 84.8% and 43.1%, and with Q-MEAN Z-scores of -1.55 and -3.81, respectively. Q-MEAN Z-score is a useful measure for the description of the absolute quality of theoretical models and is a valuable measure for identifying significant errors. Q-MEAN Z-scores less than -4.0 indicate that any part of the protein structure is not modelled correctly. These results imply a very accurate model for bovine cathepsin B and a relatively accurate model for barley HvPap-19. Major differences may be assumed from the models (Fig. 2a). The occluding loop of B cathepsins is clearly higher in the bovine cathepsin B than in HvPap-19. To know if this difference could be a common feature to other animal and plant B cathepsins, an alignment of their complete amino acid sequences was done (Fig. S1). From this alignment, two main conclusions can be reached. First, the occluding loop of plant B cathepsins is shorter than that from animals due to the absence of several amino acid residues in this region (Fig. 2b). Second, an insertion of two amino acid residues in the propeptide region near the occluding loop is conserved in all plant cathepsin B-like sequences (Fig. 2b). The lack of inhibition of a plant cathepsin B propeptide towards an animal cathepsin B can be explained from these two features. In the plant protein, there would not be steric problems between the two additional amino acid residues of the propeptide and the short occluding loop. However, a steric clash would occur between the amino acid residues Y59 of the HvPap-19 propeptide and V174 located at the occluding loop of the bovine cathepsin B (Fig. 2c).
(a) Homology models of bovine and barley HvPap-19 cathepsin B peptidases created using SWISS-MODEL. The propeptides (green) and the mature enzymes (blue; catalytic triad residues in red) are shown. Arrows mark the occluding loop domains. (b) Alignment of the amino acid regions involved in the interaction between the occluding loop and the propeptide in different animal and plant species. Alignment was generated using the MUSCLE program. The locations of residues potentially involved in steric clashes (red boxes) are indicated. Pp, Physcomitrella patens; Sm, Selaginella moellendorffii; Os, Oryza sativa; Hv, Hordeum vulgare; Pt, Populus trichocarpa; At, Arabidopsis thaliana. (c) Ribbon plots showing the structural overlay of three-dimensional models for bovine cathepsin B (orange) and HvPap-19 (green). Close-up image shows the molecular surface of V174 located in the occluding loop of bovine cathepsin B and Y59 in the propeptide of HvPap-19, which are potentially involved in a steric clash.
Structural explanation for differential barley cathepsin L inhibition
To explain the differences of inhibition of barley propeptides on barley cathepsin L-like activities, the structures of the HvPap-4, -6, -10, and -16 proteins were modelled using the crystallographic structure of the papaya cathepsin L-like procaricain as a template (Fig. 3a). Barley HvPap-4, -6, -10, and -16 aligned to procaricain at sequence identities of 50.5%, 46.8%, 43.8% and 34.8%, and with Q-MEAN Z-scores of -3.37, -2.88, -2.91 and -5.47, respectively. These results imply relatively accurate models for barley HvPap-4, -6, and -10 peptidases, and suggest that there is something incorrect in the predicted structure of HvPap-16. A model of HvPap-16 using the mature sequence of the peptidase on the above template had a Q-MEAN Z-score of -3.03, indicating that the HvPap-16 propeptide contributes significantly to the overall model error. An alignment of the propeptide amino acid sequences of the four barley cathepsin L-like proteins show that the conserved propeptide signatures of L cathepsins, the ERFNIN and GNFD motifs, are shared by all (Fig. 3b). However, the propeptide of HvPap-16 has an extension in their C-terminal part in relation to the other propeptides. When the propeptide of HvPap-16 was modelled without the extra amino acid residues that appear in its C-terminal region and forms an additional ß-sheet, the Q-MEAN Z-score was -2.09, which indicates that the wrong part of the molecule should be located in this additional ß-sheet.
(a) Homology models of barley cathepsin L-like peptidases created using SWISS-MODEL. The propeptides (green) and their corresponding mature enzymes (blue; catalytic triad residues in red) are shown. (b) Alignment of the amino acid regions of the propeptides from barley L cathepsins. Alignment was generated using the MUSCLE program. The location of residues belonging to the conserved ERFNIN and GNFD motifs (red and green boxes), and the position of a variable key amino acid putatively involved in the interaction propeptide-HvPap-10 enzyme (orange box) are indicated. (c) Ribbon plots showing the structural overlay of three-dimensional models for propeptides from HvPap-4 (green), HvPap-6 (orange), HvPap-10 (blue) and HvPap-16 (purple) peptidases and their interaction with the homology model for HvPap-10 (red). Amino acid residues in position 99 (HvPap-10 numbering) are depicted in stick mode. Close-up image shows the molecular surface of K277 of HvPap-10 peptidase, which is potentially involved in a steric clash with the side chain of H108 of the HvPap-16pro.
Predicted models show some structural differences among the mature enzymes. However, these differences seem not be critical to avoid the interaction among the mature enzymes and the propeptides. Thus, inhibitory Ki values should be explained by differences in the interaction of some key amino acid residues. An example of how spatial changes in the orientation of the side chain of one amino acid can hinder the interaction propeptide-peptidase in presented (Fig. 3c). HvPap-10 activity is strongly inhibited by HvPap-4pro and HvPap-6pro, weaker by its own HvPap-10 propeptide, and no inhibited by HvPap-16pro. The spatial location of the side chain of amino acids in position 99 (HvPap-10 numbering) can putatively explain these results. HvPap-10 peptidase has a lysine residue at position 277 that located its side chain in the vicinity of the propeptide. At the same position, the four propeptides has different amino acids: Q for HvPap-10, N for HvPap-6, and H for HvPap-4 and -16. Most important is their spatial orientation. As shown in Fig. 2C, the histidine of HvPap-16pro clashes with the molecular surface of K277, the glutamic acid of HvPap-10pro lies near K277, which could difficult the propeptide-enzyme interaction, whereas the side chains asparagine of HvPap-6pro and the histidine of HvPap-4pro are far from the side chain of K277, allowing their full interaction.
On the other hand, the propeptide of the cathepsin B-like HvPap-19 was able to inhibit some barley L-cathepsins. This is an unexpected result. In an attempt to explain it, the amino acid structure of HvPap-19pro was superimposed on the model structures of barley L cathepsins using the magic fit tool of the Swiss-PdbViewer program (Fig. 4). However, as magic fit is a tool that permits only an approximation to real structures, major errors could be present. Models indicate that the propeptide of HvPap-19 accommodates with quite accuracy on the structure of the barley cathepsins L, but differences in their inhibitory capacity cannot be deduced from the superimposed structures.
Cysteine peptidases propeptides have been suggested to be potent inhibitors with the ability to control animal parasites and plant pests. Thus, C1A cysteine peptidases of Trypanosoma cruzi or Plasmodium falciparum (animal parasites) were inhibited by their cognate propeptides , . Moreover, the cysteine peptidase activities of midgut crude soluble extracts from Colorado potato beetle or several bruchids (plant pests) were also inhibited by papaya proteinase IV propeptide or by the propeptide of a cysteine peptidase from the bean bruchid Acanthoscelides obtectus , . Recently, it was demonstrated that genetically modified soybean expressing the propeptide of a C1A peptidase from Heterodera glycines (plant nematode pest) reduced soybean cyst nematode infection . Thus, an understanding of the interaction mechanisms involving propeptides and peptidases can allow the development of complementary inhibitors that can achieve broad-spectrum inhibition of parasites and pests.
Propeptides of C1A cysteine peptidases have been shown as tight-binding inhibitors of their cognate enzymes, but also of related peptidases . Thus, to fully address the selectivity of propeptide inhibition both intraspecific and interspecific inhibitory effects has to be considered . To know the capacity of inhibition of plant propeptides, we investigated the selectivity of barley propeptides from C1A cathepsin L and B-like cysteine peptidases. As expected, competitive inhibition was determined to all propeptide-peptidase assays, confirming the putative binding of barley propeptides to the active site of the cysteine peptidases.
As recombinant cathepsins B from plants have not been purified yet, the interspecific inhibitory effects of propeptides on commercial bovine cathepsin B was tested. The occluding loop of cathepsin B has been shown to prevent the propeptide of L-like cathepsins from binding the active site , but its intrinsic flexibility permits the interaction with its own propeptide . As expected, barley propeptides from cathepsin L-like peptidases were not able to inhibit bovine cathepsin B. Surprisingly; neither the barley cathepsin B propeptide can inhibit it. Structural features may explain this result. As previously shown , the occluding loop of cathepsins B from animals is longer to that of plants. Besides, an insertion in the propeptide sequence of cathepsins B from plants is not present in that from animals. Both features, supported by molecular modelled three-dimensional structures, suggest the existence of steric impediments to enter the plant propeptide into the active site of the bovine cathepsin B.
For cathepsin L-like peptidases, we used the barley members previously purified and characterized . Inhibitory assays indicate that all propeptides are not able to inhibit all barley L cathepsins. Likewise, as occurred in human cathepsin L-like enzymes  not all propeptides are better inhibitors of their cognate enzymes than the non cognate ones. Structural features must be involved in the specificity in the interactions. As an example, steric clashes observed in the modelled three-dimensional structures can explain the higher inhibition of HvPap-10 activity exerted by propeptides from HvPap-4 and -6 peptidases than that by its cognate propeptide. In addition, several propeptides can efficiently inhibit peptidases that belong to different cathepsin subfamilies, as the falcipain-2 (cathepsin L-like enzyme) propeptide that is able to inhibit cathepsin L- and B-like peptidases . Similarly, the propeptide of barley cathepsin B-like HvPap-19 was able to inhibit barley cathepsin L-like peptidases.
In conclusion, selectivity of interaction between plant C1A cysteine peptidases and propeptides becomes a complex feature. Molecular modelling of three-dimensional protein structures has become a powerful tool to explain in broad sense the specificity in the interactions. However, as side chain packing is the most difficult part of comparative modelling, further assays should be done to fully understand propeptide/peptidase interactions in order to use plant propeptides as regulators of C1A cysteine peptidases in biotechnological systems.
Materials and Methods
The cDNA fragments spanning the putative propeptide sequences (HvPap-4pro, R25-D134; HvPap-6pro, A25-E131; HvPap-10pro, I29-D133; HvPap-17pro, A23-V156; HvPap-19pro, A20-Q95) were derived from HvPap-4, -6, -10, and -17 barley genes . These sequences were amplified by PCR and inserted in-frame into the expression vector pRSETB (Invitrogen). Propeptides were expressed and purified as recombinant proteins following the method described in . pRSETB expression plasmids containing the propeptide sequence were introduced into E. coli BL21 CodonPlus (Stratagene). Bacterial cells were grown at 37°C to an OD550 of ca. 0.5 and induced with 1 mM IPTG (isopropyl β-D-thiogalactopyranoside) for 2 h 30 min, harvested and processed. The fusion proteins with the histidine tail were purified using a His-Bind Resin (Novagen) following the manufacturer's instructions. Purification process was checked by SDS-PAGE. The final protein concentration was quantified by the BioRad kit with bovine serum albumin (BSA) as standard. Additional MALDI-TOF analysis was performed to check molecular mass and propeptide identity by peptide mass fingerprint after trypsin digestion.
Recombinant barley HvPap-4, -6, -10, -16 cysteine peptidases were purified and activated from E. coli cultures as described . The recombinant propeptides were assayed against these peptidases and commercial bovine cathepsin B (Calbiochem). Briefly, different concentrations of propeptides plus the corresponding peptidase were incubated in a buffer containing 100 mM sodium phosphate pH 6.0, 10 mM L-cysteine, 10 mM EDTA and 0.01% (v/v) Brij35 at room temperature for 10 min. Then, the Z-FR-AMC (for cathepsin L) or Z-RR-AMC (for cathepsin B) fluorescent substrates were added and the reactions were incubated for 1 h at 30°C. Emitted fluorescence was measured with a microplate fluorescence reader (Tecan GeniusPro) using an excitation filter of 365 nm and an emission filter of 465 nm. The system was calibrated with known amounts of AMC hydrolysis product in a standard reaction mixture. All assays were carried out in triplicate and blanks were used to account for the spontaneous breakdown of substrates. As negative control, proteins from E. coli transformed with the empty expression vector were used. Enzyme concentrations were determined by active-site titration with the irreversible inhibitor L-trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64). Similarly, the concentration of correctly folded propeptides was determined by titration with different barley papain-like peptidases previously titrated with E-64. The kind of inhibition was determined from Lineweaver-Burk plots (1/V versus 1/[S]), and confirmed by lineal correspondence between IC50 and [E] values and IC50 and [S] values . Apparent Ki [Ki(app)] values were calculated by non-linear regression using the GraFit program , according to the Morrison equation. The inhibition constants (Ki) were then calculated according to the equation Ki = Ki(app)/(1+[S]/Km) using Km values calculated by non-linear regression of the Michaelis-Menten equation using the GraFit program.
Alignments of propeptide/peptidase sequences
The amino acid sequences of barley C1A cysteine peptidases were extracted from the NCBI GenBank. BlastP searches for cathepsin B-like cysteine peptidases were made using the amino acid sequence of the HvPap-19 protein . Animal and plant proteins that conserve the specific features for cathepsin B peptidases were selected. Information about protein models is compiled in Table S1. Alignments of the amino acid sequences were performed using the default parameters of MUSCLE . Depicted alignments were obtained by the multiple alignment editor Jalview version 2.6 .
Molecular modelling of propeptide-cathepsin interaction
The three-dimensional structures of the barley cysteine peptidases and the bovine cathepsin B were modelled using the standard automated routine of SWISS-MODEL program . The known crystal structures of the cathepsin L-like peptidase from papaya, procaricain (PDB identifier 1PCI) and the cathepsin B from human (PDB identifier 3PBH) were used to construct the homology-based models. The template structures were selected on the basis of highest sequence similarities. Models were evaluated with the QMEAN Z-score for predicting the absolute quality of a model . The Swiss-PdbViewer program  was used to generate the single and superimposed images of protein models.
Comparison of the amino acid sequences of the cathepsin B-like cysteine proteases. The alignment was generated using the MUSCLE program. Pp, Physcomitrella patens; Sm, Selaginella moellendorffii; Os, Oryza sativa; Hv, Hordeum vulgare; Pt, Populus trichocarpa; At, Arabidopsis thaliana.
We thank Julia Kehr and the personnel of the proteomics facility of the Centro de Biotecnología y Genómica de Plantas (CBGP) for their technical support.
Conceived and designed the experiments: ID MM. Performed the experiments: IC DH. Analyzed the data: IC MM. Contributed reagents/materials/analysis tools: ID MM. Wrote the paper: ID MM.
- 1. van der Hoorn RA (2008) Plant proteases: from phenotypes to molecular mechanisms. Annu Rev Plant Biol 59: 191–223.RA van der Hoorn2008Plant proteases: from phenotypes to molecular mechanisms.Annu Rev Plant Biol59191223
- 2. Rawlings ND, Barrett AJ, Bateman A (2010) MEROPS: the peptidase database. Nucleic Acids Res 38: D227–233.ND RawlingsAJ BarrettA. Bateman2010MEROPS: the peptidase database.Nucleic Acids Res38D227233
- 3. Martinez M, Diaz I (2008) The origin and evolution of plant cystatins and their target cysteine proteinases indicate a complex functional relationship. BMC Evol Biol 8: 198.M. MartinezI. Diaz2008The origin and evolution of plant cystatins and their target cysteine proteinases indicate a complex functional relationship.BMC Evol Biol8198
- 4. Wiederanders B (2003) Structure-function relationships in class CA1 cysteine peptidase propeptides. Acta Biochim Pol 50: 691–713.B. Wiederanders2003Structure-function relationships in class CA1 cysteine peptidase propeptides.Acta Biochim Pol50691713
- 5. Demidyuk IV, Shubin AV, Gasanov EV, Kostrov SV (2010) Propeptides as modulators of functional activity of proteases. BioMolecular Concepts 1: 305–322.IV DemidyukAV ShubinEV GasanovSV Kostrov2010Propeptides as modulators of functional activity of proteases.BioMolecular Concepts1305322
- 6. Coulombe R, Grochulski P, Sivaraman J, Menard R, Mort JS, et al. (1996) Structure of human procathepsin L reveals the molecular basis of inhibition by the prosegment. EMBO J 15: 5492–5503.R. CoulombeP. GrochulskiJ. SivaramanR. MenardJS Mort1996Structure of human procathepsin L reveals the molecular basis of inhibition by the prosegment.EMBO J1554925503
- 7. Podobnik M, Kuhelj R, Turk V, Turk D (1997) Crystal structure of the wild-type human procathepsin B at 2.5 A resolution reveals the native active site of a papain-like cysteine protease zymogen. J Mol Biol 271: 774–788.M. PodobnikR. KuheljV. TurkD. Turk1997Crystal structure of the wild-type human procathepsin B at 2.5 A resolution reveals the native active site of a papain-like cysteine protease zymogen.J Mol Biol271774788
- 8. Grudkowska M, Zagdanska B (2004) Multifunctional role of plant cysteine proteinases. Acta Biochim Pol 51: 609–624.M. GrudkowskaB. Zagdanska2004Multifunctional role of plant cysteine proteinases.Acta Biochim Pol51609624
- 9. Turk V, Stoka V, Vasiljeva O, Renko M, Sun T, et al. (2012) Cysteine cathepsins: From structure, function and regulation to new frontiers. Biochim Biophys Acta 1824: 68–88.V. TurkV. StokaO. VasiljevaM. RenkoT. Sun2012Cysteine cathepsins: From structure, function and regulation to new frontiers.Biochim Biophys Acta18246888
- 10. Wiederanders B, Kaulmann G, Schilling K (2003) Functions of propeptide parts in cysteine proteases. Curr Protein Pept Sci 4: 309–326.B. WiederandersG. KaulmannK. Schilling2003Functions of propeptide parts in cysteine proteases.Curr Protein Pept Sci4309326
- 11. Menard R, Carmona E, Takebe S, Dufour E, Plouffe C, et al. (1998) Autocatalytic processing of recombinant human procathepsin L. Contribution of both intermolecular and unimolecular events in the processing of procathepsin L in vitro. J Biol Chem 273: 4478–4484.R. MenardE. CarmonaS. TakebeE. DufourC. Plouffe1998Autocatalytic processing of recombinant human procathepsin L. Contribution of both intermolecular and unimolecular events in the processing of procathepsin L in vitro.J Biol Chem27344784484
- 12. Pungercar JR, Caglic D, Sajid M, Dolinar M, Vasiljeva O, et al. (2009) Autocatalytic processing of procathepsin B is triggered by proenzyme activity. FEBS J 276: 660–668.JR PungercarD. CaglicM. SajidM. DolinarO. Vasiljeva2009Autocatalytic processing of procathepsin B is triggered by proenzyme activity.FEBS J276660668
- 13. Gutierrez-Gonzalez LH, Rojo-Dominguez A, Cabrera-Gonzalez NE, Perez-Montfort R, Padilla-Zuniga AJ (2006) Loosely packed papain prosegment displays inhibitory activity. Arch Biochem Biophys 446: 151–160.LH Gutierrez-GonzalezA. Rojo-DominguezNE Cabrera-GonzalezR. Perez-MontfortAJ Padilla-Zuniga2006Loosely packed papain prosegment displays inhibitory activity.Arch Biochem Biophys446151160
- 14. Taylor MA, Baker KC, Briggs GS, Connerton IF, Cummings NJ, et al. (1995) Recombinant pro-regions from papain and papaya proteinase IV-are selective high affinity inhibitors of the mature papaya enzymes. Protein Eng 8: 59–62.MA TaylorKC BakerGS BriggsIF ConnertonNJ Cummings1995Recombinant pro-regions from papain and papaya proteinase IV-are selective high affinity inhibitors of the mature papaya enzymes.Protein Eng85962
- 15. Martinez M, Cambra I, Carrillo L, Diaz-Mendoza M, Diaz I (2009) Characterization of the entire cystatin gene family in barley and their target cathepsin L-like cysteine-proteases, partners in the hordein mobilization during seed germination. Plant Physiol 151: 1531–1545.M. MartinezI. CambraL. CarrilloM. Diaz-MendozaI. Diaz2009Characterization of the entire cystatin gene family in barley and their target cathepsin L-like cysteine-proteases, partners in the hordein mobilization during seed germination.Plant Physiol15115311545
- 16. Martinez M, Rubio-Somoza I, Carbonero P, Diaz I (2003) A cathepsin B-like cysteine protease gene from Hordeum vulgare (gene CatB) induced by GA in aleurone cells is under circadian control in leaves. J Exp Bot 54: 951–959.M. MartinezI. Rubio-SomozaP. CarboneroI. Diaz2003A cathepsin B-like cysteine protease gene from Hordeum vulgare (gene CatB) induced by GA in aleurone cells is under circadian control in leaves.J Exp Bot54951959
- 17. Lalmanach G, Lecaille F, Chagas JR, Authie E, Scharfstein J, et al. (1998) Inhibition of trypanosomal cysteine proteinases by their propeptides. J Biol Chem 273: 25112–25116.G. LalmanachF. LecailleJR ChagasE. AuthieJ. Scharfstein1998Inhibition of trypanosomal cysteine proteinases by their propeptides.J Biol Chem2732511225116
- 18. Pandey KC, Barkan DT, Sali A, Rosenthal PJ (2009) Regulatory elements within the prodomain of Falcipain-2, a cysteine protease of the malaria parasite Plasmodium falciparum. PLoS One 4: e5694.KC PandeyDT BarkanA. SaliPJ Rosenthal2009Regulatory elements within the prodomain of Falcipain-2, a cysteine protease of the malaria parasite Plasmodium falciparum.PLoS One4e5694
- 19. Visal S, Taylor MA, Michaud D (1998) The proregion of papaya proteinase IV inhibits Colorado potato beetle digestive cysteine proteinases. FEBS Lett 434: 401–405.S. VisalMA TaylorD. Michaud1998The proregion of papaya proteinase IV inhibits Colorado potato beetle digestive cysteine proteinases.FEBS Lett434401405
- 20. Silva FB, Monteiro AC, Del Sarto RP, Marra BM, Dias SC, et al. (2007) Proregion of Acanthoscelides obtectus cysteine proteinase: a novel peptide with enhanced selectivity toward endogenous enzymes. Peptides 28: 1292–1298.FB SilvaAC MonteiroRP Del SartoBM MarraSC Dias2007Proregion of Acanthoscelides obtectus cysteine proteinase: a novel peptide with enhanced selectivity toward endogenous enzymes.Peptides2812921298
- 21. Marra BM, Souza DS, Aguiar JN, Firmino AA, Sarto RP, et al. (2009) Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots. Peptides 30: 825–831.BM MarraDS SouzaJN AguiarAA FirminoRP Sarto2009Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots.Peptides30825831
- 22. Guay J, Falgueyret JP, Ducret A, Percival MD, Mancini JA (2000) Potency and selectivity of inhibition of cathepsin K, L and S by their respective propeptides. Eur J Biochem 267: 6311–6318.J. GuayJP FalgueyretA. DucretMD PercivalJA Mancini2000Potency and selectivity of inhibition of cathepsin K, L and S by their respective propeptides.Eur J Biochem26763116318
- 23. Tsuji A, Kikuchi Y, Ogawa K, Saika H, Yuasa K, et al. (2008) Purification and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus. FEBS J 275: 5429–5443.A. TsujiY. KikuchiK. OgawaH. SaikaK. Yuasa2008Purification and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus.FEBS J27554295443
- 24. Schilling K, Korner A, Sehmisch S, Kreusch A, Kleint R, et al. (2009) Selectivity of propeptide-enzyme interaction in cathepsin L-like cysteine proteases. Biol Chem 390: 167–174.K. SchillingA. KornerS. SehmischA. KreuschR. Kleint2009Selectivity of propeptide-enzyme interaction in cathepsin L-like cysteine proteases.Biol Chem390167174
- 25. Abraham Z, Martinez M, Carbonero P, Diaz I (2006) Structural and functional diversity within the cystatin gene family of Hordeum vulgare. J Exp Bot 57: 4245–4255.Z. AbrahamM. MartinezP. CarboneroI. Diaz2006Structural and functional diversity within the cystatin gene family of Hordeum vulgare.J Exp Bot5742454255
- 26. Copeland R (2000) Enzymes: a practical introduction to structure, mechanims, and data analysis. New York: Wiley-VCH. 401 p.R. Copeland2000Enzymes: a practical introduction to structure, mechanims, and data analysisNew YorkWiley-VCH401
- 27. Leatherbarrow RJ (2009) GraFit Version 7. 7.0 ed. Horley, UK: Erithacus Software Limited. RJ Leatherbarrow2009GraFit Version 7. 7.0 edHorley, UKErithacus Software Limited
- 28. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792–1797.RC Edgar2004MUSCLE: multiple sequence alignment with high accuracy and high throughput.Nucleic Acids Res3217921797
- 29. Waterhouse AM, Procter JB, Martin DM, Clamp M, Barton GJ (2009) Jalview Version 2–a multiple sequence alignment editor and analysis workbench. Bioinformatics 25: 1189–1191.AM WaterhouseJB ProcterDM MartinM. ClampGJ Barton2009Jalview Version 2–a multiple sequence alignment editor and analysis workbench.Bioinformatics2511891191
- 30. Bordoli L, Kiefer F, Arnold K, Benkert P, Battey J, et al. (2009) Protein structure homology modeling using SWISS-MODEL workspace. Nat Protoc 4: 1–13.L. BordoliF. KieferK. ArnoldP. BenkertJ. Battey2009Protein structure homology modeling using SWISS-MODEL workspace.Nat Protoc4113
- 31. Benkert P, Biasini M, Schwede T (2011) Toward the estimation of the absolute quality of individual protein structure models. Bioinformatics 27: 343–350.P. BenkertM. BiasiniT. Schwede2011Toward the estimation of the absolute quality of individual protein structure models.Bioinformatics27343350
- 32. Guex N, Diemand A, Peitsch MC (1999) Protein modelling for all. Trends Biochem Sci 24: 364–367.N. GuexA. DiemandMC Peitsch1999Protein modelling for all.Trends Biochem Sci24364367