Bacteria associated with three coral species, Acropora tenuis, Pocillopora damicornis and Tubastrea faulkneri, were assessed before and after coral mass spawning on Ningaloo Reef in Western Australia. Two colonies of each species were sampled before and after the mass spawning event and two additional samples were collected for P. damicornis after planulation. A variable 470 bp region of the 16 S rRNA gene was selected for pyrosequencing to provide an understanding of potential variations in coral-associated bacterial diversity and community structure. Bacterial diversity increased for all coral species after spawning as assessed by Chao1 diversity indicators. Minimal changes in community structure were observed at the class level and data at the taxonomical level of genus incorporated into a PCA analysis indicated that despite bacterial diversity increasing after spawning, coral-associated community structure did not shift greatly with samples grouped according to species. However, interesting changes could be detected from the dataset; for example, α-Proteobacteria increased in relative abundance after coral spawning and particularly the Roseobacter clade was found to be prominent in all coral species, indicating that this group may be important in coral reproduction.
Citation: Ceh J, Raina J-B, Soo RM, van Keulen M, Bourne DG (2012) Coral-Bacterial Communities before and after a Coral Mass Spawning Event on Ningaloo Reef. PLoS ONE 7(5): e36920. https://doi.org/10.1371/journal.pone.0036920
Editor: Sebastian C. A. Ferse, Leibniz Center for Tropical Marine Ecology, Germany
Received: January 28, 2012; Accepted: April 15, 2012; Published: May 16, 2012
Copyright: © 2012 Ceh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: Funding provided by Australian Institute for Marine Science, Murdoch University and Western Australian Marine Science Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Corals exhibit a range of reproductive strategies, which include both sexual and asexual propagation. Brooding coral species show internal fertilization and expel well-developed larvae at various times of the year, usually over the summer months. Most corals however reproduce during annual spawning events, by broadcast spawning their gametes for external fertilisation . Mass spawning is a well known phenomenon occurring worldwide and involves the synchronous release of gametes from benthic invertebrates including scleractinian corals. The timing of coral mass spawning depends on the geographical location, and usually occurs in summer, once a year over a few nights following the full moon . Coral reproduction is regulated by several life processes such as gamete production, fertilization, planktonic larval dispersal, larval settlement, post-settlement growth, and survival. Disruption in these early life stages can result in compromised or failed recruitment and profoundly affect the distribution and survival of corals .
A stimulation of microbial processes within reef waters after episodic spawning events has previously been reported , . After a coral mass spawning event on the Great Barrier Reef (GBR), bacterial abundances in reef water increased 2-fold and remained elevated for three days, before declining to below pre-spawning values . The input of large quantities of particulate organic matter in the form of degrading gametes enhance pelagic and benthic autotrophic and heterotrophic activities , and can result in rapid oxygen depletion in the water column .
Microbes in coral reef ecosystems have been extensively studied with regard to their role in coral health and disease , coral antimicrobial properties  and their involvement in the biogeochemical cycling of nutrients , . Furthermore microbes have been suggested to co-evolve with their coral host  and to benefit the coral in adapting to environmental changes in the ecosystem . Previous studies suggested that bacterial communities in corals are distinct from those inhabiting the surrounding seawater  and that some corals harbour specific bacteria species, despite temporal or geographical separation , . Conversely, other studies showed that bacterial consortia varied with location  and time , indicating that coral–microbial community structures may be either a result of environmental drivers ,  or species- and site specific . Understanding the acquisition, maintenance and successional changes of microbial communities through different coral life stages is fundamental to understanding the functional roles these partnerships have in overall coral health.
Energy demanding physiological processes such as reproduction affect the corals metabolism which may impact its numerous microscopic partners (including Symbiodinium, Bacteria, Archaea, Fungi and viruses which form a functionally relevant mutualistic relationship with coral known as the coral holobiont) . Coral reproduction itself as well as the environmental changes associated with the large scale ecological event of coral mass spawning could potentially influence coral bacterial associates. If corals acquire bacteria according to their specific requirements in different life stages, reproduction accomplished colonies might rid themselves of bacteria associated with and important to reproduction and recruit alternative bacteria populations more suitable for the time after spawning. Coral bacteria might also change due to corals releasing large quantities of beneficial bacteria with their gametes (spawners) or planula larvae (brooders), and the re-colonization with new bacteria; or corals may simply return to pre-spawning bacterial populations as observed for temperature stressed and bleached corals .
This study investigated the diversity and community structure of coral-associated bacterial communities before and after a coral mass spawning event. Three coral species were assessed: the broadcast spawning coral A. tenuis (which participated in the synchronous event), the brooding coral P. damicornis (additional samples were collected for this species after its respective reproductive event), and the ahermatypic coral T. faulkneri. T. faulkneri does not associate with the algal symbiotic partner Symbiodinium which has previously been suggested to be involved in structuring coral microbial communities , . Like P. damicornis, the ahermatypic coral T. faulkneri broods and releases planulae and was intended to serve as an ahermatypic example. However no reproductive activity was observed through the study time (one month of observation) and the timing of reproduction for this species is unknown for the Ningaloo Reef system. Bacterial diversity was assessed by a 16 S rRNA gene pyrosequencing approach allowing for large-scale exploration of taxonomic diversity. This study is the first to investigate the dynamics of coral-microbial associates before and after coral reproductive stages.
Sample Site and Sample Collection
Three coral species, P. damicornis, A. tenuis and T. faulkneri were used in this study. Two replicated colonies per coral species were tagged and sampled on a reef flat (5–6 m water depth) near Coral Bay (23° 07′S, 113° 07′E), Ningaloo Reef, Western Australia. For each species, two pieces were collected (one from each replicate colony) two days before and two days after the coral mass spawning event in March 2009. Two additional P. damicornis colonies were removed from the reef structure and kept in an open plastic container (80×50×50 cm) on the reef flat during the day and assessed for reproductive activity on the beach at night time. The container was kept in knee deep water to maintain the ambient water temperature and returned to the reef at sunrise. P. damicornis released their planulae one week after the mass spawning event and were sampled on the reef two days after the last reproductive activity.
Two similar sized coral nubbins (approximately 2 cm in size) were removed from two coral colonies of each species using a bone clipper. Coral nubbins were placed immediately into individual, sterile zip-lock plastic bags under water and rinsed 3 times with artificial seawater (0.2 µm filtered and autoclaved) on the surface and placed on ice. The coral samples were air brushed with 2 ml of ASW to remove the coral tissue including the associated microorganisms from the coral skeleton and the tissue slurry aliquoted into cryovials. All samples were stored at −80°C until required for analysis. Samples were processed within one hour of sampling. Permits for this study were provided by the Department of Environment and Conservation.
DNA Extraction and PCR and Sequencing Preparation
Frozen tissue samples from all sampled corals were aseptically transferred to 1.5 ml Eppendorf tubes and total genomic DNA extracted using the MO BIO PowerPlant DNA Isolation Kit as per the manufacturer’s instructions (MO BIO Laboratories, CA, USA). Extracted DNA was quantified using a GeneQuant Pro spectrophotometer (Amersham Pharmacia Biotech) and stored at –20°C until required.
A 470 bp region of the 16 S ribosomal RNA gene (16 S rRNA) including the variable regions 1–3 was selected for tag pyrosequencing using the bacterial forward primer 63 F which included the primer A adaptor on the 5′ end along with a unique 8 bp barcode (5′- CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNCAGGCCTAACACATGCAAGTC) and the bacterial reverse primer 533 R with the primer B adaptor on the 5′end (5′- CCTATCCCCTGTGTGCCTTGGCAGTCTCAGTTACCGCGGCTGCTGGCAC). All amplifications were run under the following conditions: 1×Qiagen PCR Buffer (Qiagen, Germany), 1 U of HotStarTaq DNA Polymerase (Qiagen), 200 µM of each deoxynucleotide triphosphate (dNTP), 25 pmoles of each primer and MilliQ water up to 50 µl. Equal volumes of DNA (20 ng total) from each sample were used as template to generate PCR amplicons (tags). Thermocycling conditions for the amplification consisted of an initial ‘enzyme activation’ at 95°C for 5 min, followed by 30 cycles of 94°C for 1 min, 55°C for 1 min and 72°C for 1 min, followed by a final extension step of 72°C for 10 min. A total of 5 PCRs were performed for each sample and the replicate PCR’s pooled to generate more than 1 µg of template DNA. PCR products were purified using the MO Bio PCR purification kit as per the manufacturer’s instructions. Note: three of the P. damicornis (before, after spawning and after planulation) and one of the T. faulkneri samples (after spawning) failed to amplify and were excluded from subsequent analysis. The amount of DNA in each sample was quantified using the Quant-iT PicoGreen assay (Invitrogen, Carlsbad, CA). All samples with their respective bar codes (10 samples in total) were pooled in equimolar amounts for 454 pyrosequencing on a Roche GS-FLX system at the Australian Genome Research Facility (AGRF) Brisbane, Australia.
Sequence and Statistical Analyses
The sequence fasta and quality files were extracted from the raw sff output from the 454 sequence run and the sequence tag and its associated quality scores were removed. The python script split_libraries.py from the QIIME pipeline  was used to remove poor quality (<25) and short sequences (<150 bp), remove the primer and barcode, and add a sample identifier to the header of each sequence. The resulting fasta file was checked for chimeric sequences against a chimera-free database of 16 S rRNA gene sequences (Green Genes 29/11/10 release) using UCHIME . All sequences that were identified as potential chimeras were removed. Homopolymer sequence errors were corrected using ACACIA (pers.com. Dr. Gene Tyson) resulting in a chimera and error-free fasta file. The number of reads per sample was quantified for each of the previous steps (see Table 1). The number of chimera-free and error-free reads was normalised to 475 reads per sample to allow comparative diversity analysis between all samples. No significant differences (P = 0.01; 1000 permutations) were observed between the raw, cleaned and normalised datasets when PCA analysis was performed on the relative abundance of the dominant OTUs and correlation between datasets was assessed by Procrustes rotation . Therefore all analysis reported in this study was conducted on the randomly subsampled and normalised dataset. Sequences were clustered using uclust  to obtain groups of sequences at both the 90% and 97% similarity levels. These groups represent operational taxonomic units (OTUs) defined at an approximate ‘genus’ and ‘species’ levels. The QIIME pipeline was used to identify the most abundant member of each group which was subsequently chosen as the representative sequence. Sequence taxonomy was assigned using GreenGenes  and BLAST (0.75 similarity) and the QIIME pipeline was used to generate OTU tables. Alpha diversity statistics in QIIME were calculated after random sub-sampling to ensure sequencing effort did not affect diversity comparisons. Once the data set was rarefied, the following alpha-diversity metrics were generated; total observed species (OTUs) and Chao 1 diversity. Beta-diversity of the bacterial communities was analysed in using weighted UNIfrac analysis with principal components generated from the UniFrac distances and plotted in two dimensions. The pyrosequencing dataset were deposited in the NCBI Sequence Read Archive (SRA) database with the accession number (pending).
An analysis of similarities (ANOSIM) was performed to compare bacterial species diversity between coral species. A test run on R, using the package “vegan” identified significant differences in bacterial communities between coral species; the significance was computed by the permutation of the group membership, with 10,000 replicates and Bray-Curtis distance as a distance measure.
Samples collected from A. tenuis, P. damicornis and T. faulkneri before and after coral mass spawning and after planulation for P. damicornis provided a total of 11910 high quality 16 S rRNA gene sequence tags (Table 1). Chao 1 diversity index revealed the highest and lowest diversity of bacteria in T. faulkneri and P. damicornis, respectively. Bacterial diversity increased in all coral species after coral spawning, additionally bacterial diversity increased after planulae release in P. damicornis.
Replicate samples were highly similar for each time point (with the exception of the γ-Proteobacteria, Pseudoalteromonas and Shigella, which varied in their abundance between replicates in A. tenuis samples), they were therefore pooled for clarity purposes. Pooled sequence libraries provided a general overview over the ten most abundant bacteria classes associated with coral samples (Fig. 1). The γ-Proteobacteria was the dominant class of bacterial associated with all the corals. α-Proteobacteria retrieved sequences were also consistently retrieved from all the coral samples. Bacilli were consistently found in the P. damicornis corals while the Flavobacteria were consistently present in A. tenuis and T. faulkneri samples. No major shifts in coral bacterial communities were observed at the class level before and after the coral mass spawning event. This observation was consistent for all coral species and any detected variations were only minor changes in abundance of bacterial classes. A. tenuis and P. damicornis displayed more similar bacteria classes whereas sequence libraries derived from the ahermatypic coral T. faulkneri differed from the other two coral species. Coral samples grouped according to species at the class level (Fig. 1).
Replicate samples were pooled and dominant affiliations were grouped at the class level. The similarity tree was done using the neighbour-joining method and the Bray-Curtis algorithm (n = 1000 replications). Note: due to failure in amplification P. damicornis and T. faulkneri are represented by one sample per sampling point and one sample after coral spawning, respectively.
A principal component analysis (PCA) of the individual sequence data sets (unpooled samples) based on a taxonomic assignment at the genus level (>97% identity for OTU groupings) again revealed that all samples grouped according to coral species (Fig. 2) and all coral species displayed significantly different bacterial communities between each other (R = 1, rb = 6.5, rw = 35, p<0.0003). Coral replicates collected after coral spawning displayed some shifts in the coral microbial community structure when compared with samples collected directly before spawning. Furthermore slight variations in coral microbial assemblages were amplified for P. damicornis after planulation.
Sequence tags grouped into OTUs at >97% identity were considered dominant in any sample collected when present at proportions of >1%. This resulted in the 35 most abundant out of a total number of 116 OTUs, covering 92% of all retrieved sequences (Table 2). Sequence affiliations included the classes of Acidobacteria, Flavobacteria, Sphingobacteria, Bacilli, Clostridia, α-, β- and γ-Proteobacteria (Table 2). Six of 35 OTUs were present in all coral species out of which only two were found at all collection times (Table 2). A. tenuis samples consisted of 20, P. damicornis of 14 and T. faulkneri of 24 of the most abundant OTUs.
Only seventeen percent of the most abundant OTUs (five percent of all OTUs) were shared between all coral species investigated, and were associated with the α-Proteobacteria Rhodomicrobium, Roseobacter, and Rhodospirillales and the γ-Proteobacteria Shewanella, Pseudoalteromonas and Stenotrophomonas (Fig. 3).
Three coral species, A. tenuis, P. damicornis and T. faulkneri were examined to explore shifts in coral associated bacterial assemblages before and after a coral mass spawning event. Bacterial diversity increased after reproductive activity for both coral species, A. tenuis after coral spawning and P. damicornis after planulation, as indicated by Chao 1 index.
No major shifts in coral bacterial communities were observed at the class level through the coral reproduction event. This observation was consistent for all coral species which demonstrated similar microbial communities with only minor variations in abundances between bacterial classes. A more precise taxonomical assignment at the genus level (97% similarity) indicated similarities between A. tenuis and T. faulkneri in both, proportions and identities of their bacterial communities. P. damicornis however displayed differences in bacterial composition compared to the other two coral species. Only seventeen percent of the most abundant OTUs were shared between all coral species investigated, and were associated with the α-Proteobacteria Rhodomicrobium, Roseobacter, and Rhodospirillales and the γ-Proteobacteria Shewanella, Pseudoalteromonas and Stenotrophomonas Corals have previously been reported to harbour specific bacteria which differ from bacterial communities in the seawater  and the aforementioned bacteria groups seem to represent a consistent and important component in coral-bacterial associations with A. tenuis, P. damicornis and T. faulkneri, whereas other bacterial groups not found to be consistently dominant are likely to vary between coral species.
Investigating the taxonomic assignment at the genus level revealed a specific partition for bacterial classes associated with corals. For example within the family Sphingomonadaceae, the genus Erythrobacter was present in A. tenuis and in T. faulkneri only, whereas Novosphingobium was only represented in P. damicornis. Increased retrieval of sequences related to the genera Erythrobacter and Pseudoalteromonas potentially highlight their significance in A. tenuis reproduction; both genera are commonly known to associate with corals , ,  and can inhibit the growth of the coral pathogen Vibrio coralliilyticus . Furthermore, Pseudoalteromonas has previously been shown to induce coral settlement  and to possess antimicrobial properties , .
Interestingly all bacteria types affiliated with the class α-Proteobacteria either increased or remained unchanged in relative proportion of retrieved sequences after reproduction in the corals A. tenuis and P. damicornis compared to pre-spawning samples. This suggests that α-Proteobacteria may be important in coral reproduction including possible implications for the survival and increase of fitness in coral larvae. Previous work reported the genus Roseobacter to be amongst the first acquired bacteria in early developing stages of the coral Pocillopora meandrina , . Roseobacter clade affiliated bacteria species are abundant and diverse in seawater and various metabolic functions have been reported for this taxon , including antibiotic properties against coral pathogens . In the present study Roseobacter affiliated sequences are prominent in all coral species and represent the only bacteria type increasing after spawning as well as after planulation (Fig. 3), possibly providing antimicrobial activity against potentially pathogenic bacteria for coral compromised after energy demanding life stages such as spawning . These findings support the idea that Roseobacter affiliated bacteria may be specifically related to the process of reproduction in brooding as well as in spawning corals.
This is the first study to directly compare shifts in coral bacterial associations before and after spawning. Coral species displayed similar classes of bacteria, though at the genus level, small differences in associated bacterial communities were observed. Abundant OTUs potentially represent bacteria which play a role during coral reproduction since they specifically appeared before or after coral spawning or in distinctly high numbers in between sampling times. This study lays the groundwork for future research investigating potentially important functional roles of the identified bacterial groups, and their implication in coral reproduction and the early establishment of corals.
We would like to thank the Australian Institute of Marine Science, the Western Australian Marine Science Institution and Murdoch University for their contributions to this research. Paul Dennis from the University of Queensland is thanked for help with passing the pyrotag sequence dataset through the programme ACACIA. We also thank Frazer McGregor and Bernard O’Reilly for their help in the field.
Conceived and designed the experiments: JC DGB. Performed the experiments: JC RMS. Analyzed the data: JC JBR DGB. Contributed reagents/materials/analysis tools: DGB MvK. Wrote the paper: JC JBR DGB MvK.
- 1. Harrison PL, Babcock RC, Bull GD, Oliver JK, Wallace CC, et al. (1984) Mass spawning in tropical reef corals. Science 223: 1186–1188.PL HarrisonRC BabcockGD BullJK OliverCC Wallace1984Mass spawning in tropical reef corals.Science22311861188
- 2. Albright R, Langdon C (2011) Ocean acidification impacts multiple early life history processes of the Caribbean coral Porites astreoides. Global Change Biol 17: 2478–2487.R. AlbrightC. Langdon2011Ocean acidification impacts multiple early life history processes of the Caribbean coral Porites astreoides.Global Change Biol1724782487
- 3. Glud RN, Eyre BD, Patten N (2008) Biogeochemical responses to mass coral spawning at the Great Barrier Reef: Effects on respiration and primary production. Limnol Oceanogr 53: 1014–1024.RN GludBD EyreN. Patten2008Biogeochemical responses to mass coral spawning at the Great Barrier Reef: Effects on respiration and primary production.Limnol Oceanogr5310141024
- 4. Patten NL, Mitchell JG, Middelboe M, Eyre BD, Seuront L, et al. (2008) Bacterial and viral dynamics during a mass coral spawning period on the Great Barrier Reef. Aquat Mibrob Ecol 50: 209–220.NL PattenJG MitchellM. MiddelboeBD EyreL. Seuront2008Bacterial and viral dynamics during a mass coral spawning period on the Great Barrier Reef.Aquat Mibrob Ecol50209220
- 5. Wild C, Jantzen C, Struck U, Hoegh-Guldberg O, Huettel M (2008) Biogeochemical responses following coral mass spawning on the Great Barrier Reef; pelagic-benthic coupling. Coral Reefs 27: 123–132.C. WildC. JantzenU. StruckO. Hoegh-GuldbergM. Huettel2008Biogeochemical responses following coral mass spawning on the Great Barrier Reef; pelagic-benthic coupling.Coral Reefs27123132
- 6. Simpson CJ, Cary JL, Masini RJ (1993) Destruction of corals and other reef animals by coral spawn slicks on Ningaloo Reef, Western Australia. Coral Reefs 12: 185–191.CJ SimpsonJL CaryRJ Masini1993Destruction of corals and other reef animals by coral spawn slicks on Ningaloo Reef, Western Australia.Coral Reefs12185191
- 7. Rosenberg E, Koren O, Reshef L, Efrony R, Zilber-Rosenberg I (2007) The role of microorganisms in coral health, disease and evolution. Nat Rev Microbiol 5: 355–362.E. RosenbergO. KorenL. ReshefR. EfronyI. Zilber-Rosenberg2007The role of microorganisms in coral health, disease and evolution.Nat Rev Microbiol5355362
- 8. Ritchie KB (2006) Regulation of microbial populations by coral surface mucus and mucus-associated bacteria. Mar Ecol Prog Ser 322: 1–14.KB Ritchie2006Regulation of microbial populations by coral surface mucus and mucus-associated bacteria.Mar Ecol Prog Ser322114
- 9. Olson ND, Ainsworth TD, Gates RD, Takabayashi M (2009) Diazotrophic bacteria associated with Hawaiian Montipora corals: Diversity and abundance in correlation with symbiotic dinoflagellates J Exp Mar Biol Ecol 371: 140–146.ND OlsonTD AinsworthRD GatesM. Takabayashi2009Diazotrophic bacteria associated with Hawaiian Montipora corals: Diversity and abundance in correlation with symbiotic dinoflagellates J Exp Mar Biol Ecol371140146
- 10. Raina JB, Tapiolas D, Willis BL, Bourne DG (2009) Coral-associated bacteria and their role in the biogeochemical cycling of sulfur. Appl Environ Microbiol 75: 3492–3501.JB RainaD. TapiolasBL WillisDG Bourne2009Coral-associated bacteria and their role in the biogeochemical cycling of sulfur.Appl Environ Microbiol7534923501
- 11. Reshef L, Koren O, Loya Y, Zilber-Rosenberg I, Rosenberg E (2006) The coral probiotic hypothesis Environ Microbiol 8: 2068–2073.L. ReshefO. KorenY. LoyaI. Zilber-RosenbergE. Rosenberg2006The coral probiotic hypothesis Environ Microbiol820682073
- 12. Bourne D, Munn C (2005) Diversity of bacteria associated with the coral Pocillopora damicornis from the Great Barrier Reef. Environ Microbiol 7: 1162–1174.D. BourneC. Munn2005Diversity of bacteria associated with the coral Pocillopora damicornis from the Great Barrier Reef.Environ Microbiol711621174
- 13. Frias-Lopez J, Zerkle AL, Bonheyo GT, Fouke BW (2002) Partitioning of bacterial communities between seawater and healthy, black band diseased, and dead coral surfaces. Appl Environ Microbiol 68: 2214–2228.J. Frias-LopezAL ZerkleGT BonheyoBW Fouke2002Partitioning of bacterial communities between seawater and healthy, black band diseased, and dead coral surfaces.Appl Environ Microbiol6822142228
- 14. Rohwer F, Segritan V, Azam F, Knowlton N (2002) Diversity and distribution of coral-associated bacteria. Mar Ecol Prog Ser 243: 1–10.F. RohwerV. SegritanF. AzamN. Knowlton2002Diversity and distribution of coral-associated bacteria.Mar Ecol Prog Ser243110
- 15. Littman RA, Willis BL, Pfeffer C, Bourne DG (2009) Diversity of coral-associated bacteria differ with location but not species for three Acroporids on the Great Barrier Reef. FEMS Microbiology Letters 68: 152–163.RA LittmanBL WillisC. PfefferDG Bourne2009Diversity of coral-associated bacteria differ with location but not species for three Acroporids on the Great Barrier Reef.FEMS Microbiology Letters68152163
- 16. Ceh J, van Keulen M, Bourne DG (2011) Coral-associated bacterial communities on Ningaloo Reef, Western Australia. FEMS Microbiol Ecol 75: 134–144.J. CehM. van KeulenDG Bourne2011Coral-associated bacterial communities on Ningaloo Reef, Western Australia.FEMS Microbiol Ecol75134144
- 17. Hong MJ, Yu YT, Chen CA, Chiang PW, Tang SL (2009) Influence of Species Specificity and Other Factors on Bacteria Associated with the Coral Stylophora pistillata in Taiwan. Appl Environ Microbiol 75: 7797–7806.MJ HongYT YuCA ChenPW ChiangSL Tang2009Influence of Species Specificity and Other Factors on Bacteria Associated with the Coral Stylophora pistillata in Taiwan.Appl Environ Microbiol7577977806
- 18. Sunagawa S, Woodley CM, Medina M (2010) Threatened Corals Provide Underexplored Microbial Habitats. PLoSONE 5: e9554.S. SunagawaCM WoodleyM. Medina2010Threatened Corals Provide Underexplored Microbial Habitats.PLoSONE5e9554
- 19. Bourne DG, Iida Y, Uthicke S, Smith-Keune C (2008) Changes in coral-associated microbial communities during a bleaching event. ISME J 2: 350–363.DG BourneY. IidaS. UthickeC. Smith-Keune2008Changes in coral-associated microbial communities during a bleaching event.ISME J2350363
- 20. Banin E, Khare SK, Naider F, Rosenberg E (2001) Proline-Rich Peptide from the Coral Pathogen Vibrio shiloi that Inhibits Photosynthesis of Zooxanthellae. Appl Environ Microbiol 67: 1536–1541.E. BaninSK KhareF. NaiderE. Rosenberg2001Proline-Rich Peptide from the Coral Pathogen Vibrio shiloi that Inhibits Photosynthesis of Zooxanthellae.Appl Environ Microbiol6715361541
- 21. Raina JB, Dinsdale EA, Willis BL, Bourne DG (2010) Do the organic sulfur compounds DMSP and DMS drive coral microbial associations? Trends in Microbiology 18: 101–108.JB RainaEA DinsdaleBL WillisDG Bourne2010Do the organic sulfur compounds DMSP and DMS drive coral microbial associations?Trends in Microbiology18101108
- 22. Caporaso G, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, et al. (2010) QIIME allows analysis of high-throughput community sequencing data. Nature 7: 335–336.G. CaporasoJ. KuczynskiJ. StombaughK. BittingerFD Bushman2010QIIME allows analysis of high-throughput community sequencing data.Nature7335336
- 23. Edgar RC, Haas BJ, C JC, Quince C, Knight R (2011) UCHIME improves sensitivity and speed of chimera detection. Bioinformatics. RC EdgarBJ HaasJC CC. QuinceR. Knight2011UCHIME improves sensitivity and speed of chimera detection.Bioinformatics
- 24. Gower JC, Dijksterhuis GB (2004) Procrustes problems: Oxford University Press. JC GowerGB Dijksterhuis2004Procrustes problems: Oxford University Press
- 25. Edgar RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics. pp. 2460–2461.RC Edgar2010Search and clustering orders of magnitude faster than BLAST.Bioinformatics24602461
- 26. DeSantis TZ, Hugenholtz P, Larsen N, Rojas M, Brodie EL, et al. (2006) Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB. Appl Environ Microbiol. pp. 5069–5072.TZ DeSantisP. HugenholtzN. LarsenM. RojasEL Brodie2006Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB.Appl Environ Microbiol50695072
- 27. Rohwer F, Breitbart M, Jara J, Azam F, Knowlton N (2001) Diversity of bacteria associated with the Caribbean coral Monastera franksi. Coral Reefs 20: 85–91.F. RohwerM. BreitbartJ. JaraF. AzamN. Knowlton2001Diversity of bacteria associated with the Caribbean coral Monastera franksi.Coral Reefs208591
- 28. Vizcaino MI, Johnson WR, Kimes ME, Williams K, Torralba M, et al. (2010) Antimicrobial Resistance of the coral pathogen Vibrio coralliilyticus and Caribbean Sister Phylotypes Isolated from a Diseased Octocoral. Environ Microbiol 59: 646–657.MI VizcainoWR JohnsonME KimesK. WilliamsM. Torralba2010Antimicrobial Resistance of the coral pathogen Vibrio coralliilyticus and Caribbean Sister Phylotypes Isolated from a Diseased Octocoral.Environ Microbiol59646657
- 29. Webster NS, Smith LD, Heyward AJ, Watts JEM, Webb RI, et al. (2004) Metamorphosis of a Scleractinian Coral in Response to Microbial Biofilms. Appl Environm Microbiology 70: 1213–1221.NS WebsterLD SmithAJ HeywardJEM WattsRI Webb2004Metamorphosis of a Scleractinian Coral in Response to Microbial Biofilms.Appl Environm Microbiology7012131221
- 30. Shnit-Orland M, Kushmaro A (2009) Coral mucus-associated bacteria:a possible first line of defense. FEMS Microbiol Ecol 67: 371–380.M. Shnit-OrlandA. Kushmaro2009Coral mucus-associated bacteria:a possible first line of defense.FEMS Microbiol Ecol67371380
- 31. Apprill A, Marlow HQ, Martindale MQ, Rappe MS (2009) The onset of microbial associations in the coral Pocillopora meandrina. ISME J 3: 685–699.A. ApprillHQ MarlowMQ MartindaleMS Rappe2009The onset of microbial associations in the coral Pocillopora meandrina.ISME J3685699
- 32. Sharp KH, Distel DL, Paul VJ (2011) Diversity and dynamics of bacterial communities in early life stages of the Caribbean coral Porites astreoides. ISME J. KH SharpDL DistelVJ Paul2011Diversity and dynamics of bacterial communities in early life stages of the Caribbean coral Porites astreoides.ISME J
- 33. Piekarski T, Buchholz I, Schobert M, Wagner-Doebler I, Tielen P (2009) Genetic tools for the investigation of Roseobacter clade bacteria. Bmc Microbiol 9: T. PiekarskiI. BuchholzM. SchobertI. Wagner-DoeblerP. Tielen2009Genetic tools for the investigation of Roseobacter clade bacteria.Bmc Microbiol9
- 34. Nissimov J, Rosenberg E, Munn CB (2009) Antimicrobial properties of resident coral mucus bacteria of Oculina patagonica. FEMS Microbiol Letters 292: 210–215.J. NissimovE. RosenbergCB Munn2009Antimicrobial properties of resident coral mucus bacteria of Oculina patagonica.FEMS Microbiol Letters292210215
- 35. Penesyan A, Tebben J, Lee M, Thomas T, Kjelleberg J, et al. (2011) Identification of the Antibacterial Compound Produced by the Marine Epiphytic Bacterium Pseudovibrio sp. D323 and Related Sponge-Associated Bacteria. Mar Drugs. pp. 1391–1402.A. PenesyanJ. TebbenM. LeeT. ThomasJ. Kjelleberg2011Identification of the Antibacterial Compound Produced by the Marine Epiphytic Bacterium Pseudovibrio sp.D323 and Related Sponge-Associated Bacteria. Mar Drugs13911402