Phosphorus (P) is a major limiting nutrient for plant growth in many soils. Studies in model species have identified genes involved in plant adaptations to low soil P availability. However, little information is available on the genetic bases of these adaptations in vegetable crops. In this respect, sequence data for melon now makes it possible to identify melon orthologues of candidate P responsive genes, and the expression of these genes can be used to explain the diversity in the root system adaptation to low P availability, recently observed in this species.
Methodology and Findings
Transcriptional responses to P starvation were studied in nine diverse melon accessions by comparing the expression of eight candidate genes (Cm-PAP10.1, Cm-PAP10.2, Cm-RNS1, Cm-PPCK1, Cm-transferase, Cm-SQD1, Cm-DGD1 and Cm-SPX2) under P replete and P starved conditions. Differences among melon accessions were observed in response to P starvation, including differences in plant morphology, P uptake, P use efficiency (PUE) and gene expression. All studied genes were up regulated under P starvation conditions. Differences in the expression of genes involved in P mobilization and remobilization (Cm-PAP10.1, Cm-PAP10.2 and Cm-RNS1) under P starvation conditions explained part of the differences in P uptake and PUE among melon accessions. The levels of expression of the other studied genes were diverse among melon accessions, but contributed less to the phenotypical response of the accessions.
This is the first time that these genes have been described in the context of P starvation responses in melon. There exists significant diversity in gene expression levels and P use efficiency among melon accessions as well as significant correlations between gene expression levels and phenotypical measurements.
Citation: Fita A, Bowen HC, Hayden RM, Nuez F, Picó B, Hammond JP (2012) Diversity in Expression of Phosphorus (P) Responsive Genes in Cucumis melo L. PLoS ONE 7(4): e35387. https://doi.org/10.1371/journal.pone.0035387
Editor: John Schiefelbein, University of Michigan, United States of America
Received: December 22, 2011; Accepted: March 15, 2012; Published: April 19, 2012
Copyright: © 2012 Fita et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the programs of foreign mobility “Jose Castillejo” [JC2009-00147], Universitat Politecnica de Valencia Professor Mobility Program of the Universitat Politecnica de Valencia [PAID-00-10] and United Kingdom (UK) Department for the Environment, Food and Rural Affairs grant [WQ0119]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Phosphorus (P) is major limiting nutrient for plant growth , . Therefore, crops are frequently supplied with inorganic phosphate (Pi) fertilizers to maintain yields and quality. However, the application of Pi fertilizers is problematic for both the intensive and extensive agriculture of the developed and developing countries, respectively. In intensive agriculture, excess of soluble inorganic Pi fertilizers is leading to eutrophication and hypoxia of water bodies . In extensive agriculture in the tropics and subtropics, chemically imbalanced soils reduce the availability of P to crops and a lack of infrastructure and purchasing power for fertilizers compound these issues . In addition, since over 85% of mined P is used in food production  and consumption of this non-renewable resource will lead to peak phosphorus production (akin to peak oil; , ), there will be increasing pressures on Pi fertilizer availabilities and costs in the future. These pressures will be exacerbated by increasing demand on food production systems as the human population increases and by fluctuation in oil prices . Sustainable management of P in agriculture requires developing crops with enhanced P efficiency and management schemes that increase soil Pi availability . Plants have developed adaptive strategies to cope with low soil P availability. These include: i) improvement of Pi-utilization efficiency by enhancing Pi internal remobilization, transport and metabolism, and ii) improvement of Pi-acquisition efficiency by modifying root systems and mobilizing Pi from the soil , .
Over the past two decades, extensive studies of the response to P starvation using Arabidopsis thaliana as a readout phenotype have contributed markedly to the understanding of P signaling and response pathways. In addition, transcriptional profiling of A. thaliana, other globally important crops such as Oryza sativa (rice), Lupinus albus (white lupin), Phaseolus vulgaris (common bean), Brassica rapa (Chinese cabbage), Solanum tuberosum (potato) and Zea mays (maize) have extend our knowledge and revealed the complexity of the network of genes necessary for plants to adapt to low soil P availability –. These studies have revealed a series genes involved in the adaptations to low P, mainly through the regulation of P acquisition, intern remobilization, change in metabolism, and signal transduction . For instance, expression of genes encoding purple acid phosphatases (PAPs) and ribonucleases (RNS) are generally up-regulated under Pi starvation. PAPs are involved in releasing Pi from organic sources, both internally and externally, for efficient transport and subsequent use. They are important in the mobilization of the organic P in soil for root absorption, remobilization of organic P in senescing organs, storage tissues, and intracellular compartments , . In addition, several studies in arabidopsis, rice and white lupin have demonstrated how Pi starvation affects carbon metabolism , , , resulting in the accumulation of starch in the leaves and increasing anthocyanin production to protect the photosynthetic machinery. Phosphate starvation also provokes modifications in the lipid composition of plant membranes, including a decrease of phospholipids and an increase of non-phosphorous lipids. Consequently, a typical transcriptional response to Pi starvation is the up regulation of enzymes involved in sulfolipid and galactolipid synthesis –. In addition, the transcripts and activity of Pi transporters are increased to optimize uptake and remobilization of Pi in Pi-deficient plants –, , , .
Melon (Cucumis melo L.) ranks as the 9th most cultivated horticultural crop in terms of total world production. This species belongs to the botanical family Cucurbitaceae, commonly known as cucurbits. It is an important crop in tropical and subtropical areas, many of which have P-deficient soils. The species is considered to be divided into two subspecies, ssp. melo and ssp. agrestis, each one with several botanical varieties that display a rich morphological diversity . Botanical varieties belonging to the ssp. agrestis are wild or exotic types found in Africa and eastern Asia, from India to Japan, and those belonging to the ssp. melo are mainly cultivated types found from India to Europe and in the Americas. The main ssp. agrestis varieties are conomon (Thunberg) Makino, momordica (Roxburgh) Duthie & Fuller, and the main ssp. melo varieties are dudaim (L.) Naudin, flexuosus (L.) Naudin, cantalupensis Naudin, and inodorus Jacquin, with cantalupensis and inodorus containing most of the commercial varieties . Variability in root morphology and architecture has been described within this species, especially between varieties of both subspecies , . The relationships between root architecture and response to Pi starvation has also been studied, showing a high variability in the acquisition and use of Pi among melon varieties . However, to our knowledge, there is no information available on the genes involved in the Pi starvation response in melon.
The availability of genetic and genomic resources allows the use of the arabidopsis vast information on P homeostasis related genes on other species . Genomic resources for melon have increased significantly in recent years. These tools include a complete transcriptome, with 53,252 accurately annotated unigenes, assembled from a collection of 125,908 and 689,054 expressed sequence tags (ESTs) ,  and 454 sequencing methodologies from a number of accessions belonging to both subspecies . Whole genome sequence of this crop is also in progress . These recent advances make it possible to identify melon orthologues of candidate Pi responsive genes described in other species.
Here we report, diversity in the expression of Pi starvation responsive genes in nine different melon varieties with contrasting phenotypic responses to Pi starvation . Representatives of the two subspecies of melon, the main commercial groups and the exotic types mostly used in melon breeding are included.
Biomass allocation and PUE traits vary significantly between melon accessions
The biomass allocation and PUE traits were analyzed under P replete (control, C) and P starving (NoP) conditions in a genetically diverse set of melon accessions: three accessions belonging to ssp. agrestis (chi-SC and ma-YP of var. conomon, and mo-kha of var. momordica) and six accessions belonging to ssp. melo (cha-PI and flex-Ac of var. flexuosus and four varieties of the main commercial groups, re-Du of var. reticulatus, ca-NC of var. cantalupensis and In-Ps and In-Am of var. inodorus (Table 1).
There were significant differences in root fresh weight (RFW), shoot fresh weight (SFW), root to shoot ratio (RSRa) and root length (RL) among accessions within the control treatment (Figure 1). Accessions belonging to conomon group of ssp. agrestis, chi-SC and ma-YP, in general had low SFW and RFW, and high RSRa, which was not the case of mo-Kha, which also belongs to ssp. agrestis. From the melo ssp., flex-Ac had the highest SFW and RFW, followed by in-Am. Cha-PI, in-PS and re-Du had intermediate values for SFW and RFW, whereas ca-NC displayed morphological traits similar to ma-YP. The lack of P did not produced big reductions in SFW, only mo-kha and flex-Ac showed a significant reduction in SFW between C and NoP treatments (Figure 1A). Despite the tendency of increasing the RFW under NoP condition, only ma-YP, cha-PI and in-Am showed a significant increase in their RFW between C and NoP treatments (Figure 1B). However, the increase of RSRa and RL was significant for all the accessions (except for RL in chi-SC, flex-Ac and re-Du; Figure 1C and D). Although the treatment period was insufficient to significantly reduce SFW, it is clear that the plants are responding to the treatment by altering resource allocation and increasing RSRa. It is interesting to note that flex-Ac had the longest root length in the control treatment, and despite being the only accession to reduce its RL in the NoP treatment still ranked fourth for RL, following in-Am, mo-Kha, and cha-PI, in the NoP treatment (Figure 1D).
Mean (a) shoot fresh weight, (b) root fresh weight, (c) root to shoot ratio and (d) root length for melon accessions grown hydroponically with a full nutrient solution (Control, light grey bars) or nutrient solution containing no phosphate (NoP, dark grey) for 21 d. Each bar represents the mean ± se (n = 9). * The mean value in NoP treatment was significantly different at P<0.05 from the mean value of the same accession in Control treatment. ns no significant difference.
There were no significant differences among accessions for shoot P concentration (Shoot-[P], %) in control conditions (Figure 2A), but significant differences were observed for shoot P content (Shoot-P, mg P plant−1), where flex-Ac had the highest Shoot-P (9.48 mg P plant−1; Figure 2B), mo-kha, in-Am, in-PS and cha-PI where intermediate, ranging from 3.28–7.23 mg P plant−1, and chi-Sc, ma-YP and ca-NC had the lowest values (0.98 to 1.97 mg P plant−1). Under NoP conditions, the lowest Shoot-[P] was observed in mo-kha (0.19 P%) and the highest was observed in ca-NC (0.5 P%), followed by in-PS (0.39 P %; Figure 2C). For Shoot-P in NoP conditions, flex-Ac and in-Am had the highest values (1.1 and 1 mg P plant−1, respectively) and chi-SC again had the lowest value (0.11 mg P plant−1; Figure 2D). Taking into account that there was no P in the NoP treatment, these differences in Shoot-P should be considered as differences in the accumulation of P on the genotypes seeds (all seeds were produced under the same conditions in our greenhouses).
Mean (a,b) shoot P concentration and (c,d) shoot P content for melon accessions grown hydroponically with a full nutrient solution (Control, a,c) or nutrient solution containing no phosphate (NoP, b,d) for 21 d. Each bar represents the mean ± se (n = 9). Bars with the same letter are not significantly different at P<0.05 by Newmans Keuls multiple range test.
There were significant differences for P use efficiency traits among the melon accessions (Figure 3). Chi-SC, ma-YP and ca-NC had low values for PUpE and PPUE, whereas their values for PUtE were variable, including negative values for ma-YP and a high positive value for Chi-SC. Within the ssp. agrestis, mo-Kha had significantly different values for PUpE and PPUE compared with other agrestis accessions chi-SC and ma-YP (Figure 3). Within the ssp. melo, Flex-Ac had the highest values for PUpE, PUtE and PPUE under control conditions and the second highest for PPUE under the NoP treatment. The inodorus accessions also had moderate to high values for the different measures of PUE, with in-Am having the highest value for PPUE under the NoP treatment (Figure 3D). Re-Du and cha-PI, had intermediate values for PUE traits, between inodorus and conomon groups, cha-PI also had a negative value for PUtE as consequence of its higher SDW under P starvation.
Mean (a) Phosphorus (P) uptake efficiency, (b) P utilization efficiency, (c) physiological P use efficiency for melon accessions grown hydroponically with a full nutrient solution, and (d) physiological P use efficiency for melon accessions grown hydroponically with a nutrient solution containing no phosphate for 21 d. Each bar represents the mean ± se (n = 9). Bars with the same letter are not significantly different at P<0.05 by Newmans Keuls multiple range test.
Expression of genes involved in response to P starvation
The expression of genes involved in plant responses to P starvation (phosphorus uptake and mobilization, carbon and secondary metabolism, alteration of membrane lipids composition and P transport) were analyzed under control and NoP conditions in a genetically diverse set of melon accessions.
Phosphate mobilization and re-mobilization
Of the two putative purple acid phosphatases profiled (Cm-PAP10.1 and Cm-PAP10.2, ICUGI unigenes MU46092 and MU50216), the relative expression of Cm-PAP10.1 was lower than that of Cm-PAP10.2 in control conditions (Figure 4A and B). Under NoP conditions both genes were induced, with Cm-PAP10.1 having a greater fold change induction in expression than Cm-PAP10.2, with the former induced between 5 to 17-fold, and the latter induced between 1.67 to 6.71-fold (Figure 4B). The relative expression of these two genes in the studied accessions was variable. Chi-SC, re-Du and ca-NC showed low to moderate relative expression levels in both treatments and genes. Under NoP conditions, mo-Kha, cha-PI, had moderate expression of Cm-PAP10.1, but high expression of Cm-PAP10.2. In contrast, in-Am and in-PS had high expression of Cm-PAP10.1 and moderate to low expression of Cm-PAP10.2 under NoP conditions. Flex-Ac showed the highest relative expression of Cm-PAP10.1 and a moderate expression of Cm-PAP10.2 in NoP conditions. Interestingly, despite the low expression of these genes under NoP conditions for chi-SC and ma-YP accessions, they showed a greater induction in expression from control to NoP conditions (Figure 4A and B).
Relative transcript abundance for (a) Cm-PAP10.1 [MU46092], (b) Cm-PAP10.2 [MU50216], (c) Cm-RNS1 [MU47003] in different melon accessions grown hydroponically with a full nutrient solution (Control, light grey) or nutrient solution containing no phosphate (NoP, dark grey) for 21 d. Transcript abundance was measured using quantitative PCR (Q-PCR) and expressed relative to that of the housekeeping gene (Cm-Ubiquitin). Grey bars with the same grey letter are not significant different at P<0.05 by Newmans Keuls multiple range test.
Cm-RNS1 (ICUGI unigen MU47003), which encodes a ribonuclease, was also induced under NoP conditions (Figure 4C). The relative basal expression levels in control conditions were close to one, with a significant increase under NoP conditions. The change of expression was similar among accessions except for chi-SC which showed the lowest increase (7-foldhigher expression in NoP than in the control) and in-PS which showed the highest one (26-fold). The accession with the highest relative expression under NoP condition was mo-kha (31.12) followed by flex-Ac and in-Am (Figure 4C).
The expression of these targets putatively involved in P mobilization/remobilization correlated with changes in root structure and measures of PUE (Table 2). The expression of Cm-PAP10.1 was positively correlated with SFW, RFW and RL in control and NoP treatments, suggesting that higher levels of gene expression are related with higher plant size no matter the treatment. Whereas Cm-PAP10.2 and Cm-RNS1 relative expressions showed significant correlations with SFW, RFW and RL, under NoP treatments, indicating that these genes may be associated with improved biomass allocations under NoP conditions. RSRa was negatively correlated with the relative expression of Cm-PAP10.1 and Cm-PAP10.2 in control conditions, and accessions with high RSRa, such as chi-SC, ma-YP and ca-NC, had lower basal levels of expression of these genes.
The expression of Cm-PAP10.1 was also highly positively correlated with shoot P content and PPUE under control conditions but not under NoP conditions. Cm-PAP10.1 was less expressed in those accessions with small vines and roots in control conditions. These poorly performing accessions had high increases in the expression of the gene when passing form control to NoP but not great PUpE resulting in a negative correlation between PUpE and Cm-PAP10.1 induction. Interestingly Cm-PAP10.2 had different response; it has a negative correlation with [P] and a positive correlation with PPUE under NoP conditions. Therefore, it seems that Cm-PAP10.1 is correlated with better performance in control conditions and Cm-PAP10.2 is correlated with higher performance under NoP conditions. Cm-RNS1 showed similar correlations with other studied traits to Cm-PAP10.2, but in this case Cm-RNS1 was also positively correlated with PUpE, therefore it may contribute to increase uptake in presence of P but also contribute to perform better under NoP conditions.
Carbon and secondary metabolism
The expression of genes known to be involved in carbon and secondary metabolism, were also measured in all nine accessions. These included a phosphoenol pyruvate kinase, Cm-PPCK1, and an anthocyanin 5-aromatic acetyltransferase, Cm-transferase, (ICUGI unigenes MU52466 and MU47437). Under NoP conditions, the expression of CmPPCK1 increased on average 7-fold across accessions compared with the control treatment (Figure 5A). Ma-YP and ca-NC showed lower levels of expression under NoP (4.38 and 4.72 respectively) and re-Du had the highest level of expression (12.38) followed by in-PS. Under the NoP condition the expression of Cm-transferase increased on average 7-fold across accessions compared with the control treatment (Figure 5B). There were no large differences in relative expression among accessions under NoP treatment, except for ma-YP (17.81) with the greatest expression, and cha-PI with the lowest (5.33). The highest induction of expression was achieved by flex-AC with a 9-fold increase in expression (Figure 5B).
Relative transcript abundance for (a) Cm-PPCK1 [MU52466], (b) Cm-transferase [MU47437] in different melon accessions grown hydroponically with a full nutrient solution (Control, light grey) or nutrient solution containing no phosphate (NoP, dark grey) for 21 d. Transcript abundance was measured using quantitative PCR (Q-PCR) and expressed relative to that of the housekeeping gene (Cm-Ubiquitin). Grey bars with the same grey letter are not significant different at P<0.05 by Newmans Keuls multiple range test. Dark grey bars with the same letter are not significant different at P<0.05 by Newmans Keuls multiple range test.
The only significant correlation between the relative expressions of Cm-PPCK and phenotypical traits was a negative correlation with RSRa in both treatments. The expression of Cm-transferase was negatively correlated with SFW, RFW, P content and PPUE, under control conditions, indicating that higher expression of this gene under control conditions was associated with low PUE. However, the increased expression of Cm-transferase from control to NoP conditions was positively correlated with PUpE and PUtE, suggesting that this gene might be associated with changes in the efficiencies of P uptake and use under NoP conditions.
Alteration membrane lipid composition and metabolism
Genes involved in the manipulation of lipid membrane composition under Pi starvation showed increases in expression under NoP conditions relative to control conditions. The expression of Cm-DGD1 (ICUGI unigen MU 51583), a putative digalactosyl diacyglycerol synthase, increased its expression approximately 2-fold under NoP conditions (Figure 6A). The accession with highest expression under NoP was chi-SC (8.6) and the lowest were ma-YP (4.71) and flex-Ac (3.65). Cm-SQD1 (ICUGI unigene MU52028) encodes for a putative UDP sulfoquinovose synthase (SQD1) and was induced 2 to 5-fold under NoP conditions relative to control conditions (Figure 6B). Re-Du, in-Am and in-PS showed the greatest relative expression in NoP (4–5) and ma-Yp with ca-NC the lowest (2).
Relative transcript abundance for (a) Cm-DGD1 [MU51583], (b) Cm-SQD1 [MU52028] in different melon accessions grown hydroponically with a full nutrient solution (Control, light grey) or nutrient solution containing no phosphate (NoP, dark grey) for 21 d. Transcript abundance was measured using quantitative PCR (Q-PCR) and expressed relative to that of the housekeeping gene (Cm-Ubiquitin). Grey bars with the same grey letter are not significant different at P<0.05 by Newmans Keuls multiple range test. Dark grey bars with the same letter are not significant different at P<0.05 by Newmans Keuls multiple range test.
Cm-DGD1 and Cm-SQD1 changes in expression were positively correlated with PUtE, indicating that a higher increase in the expression of these genes may be involved in a higher internal P utilization. Cm-DGD1 was also negative correlated with SFW, RFW, shoot P content and PPUE in control conditions. Indicating that high biomass and PUE accessions under control conditions, have lower basal levels of expression.
Cm-SPX2 (ICUGI unigene MU43709) a putative transport protein with a SPX domain was impossible to correctly amplify ca-NC samples. Under NoP conditions, the expression of this gene was 44.5 times higher the basal expression (Figure 7). The higher change of expression was experienced by cha-PI which passed from a basal level of expression of 0.86 to an expression in NoP of 25.78, which in turn was one of the lowest levels of expression in comparison with other accessions. The higher levels of expression in NoP were reached by re-Du and in-PS 44.51 and 45 respectively (Figure 7).
Relative transcript abundance for CmSPX2 [MU43709/MU63649] in different melon accessions grown hydroponically with a full nutrient solution (Control, light grey) or nutrient solution containing no phosphate (NoP, dark grey) for 21 d. Transcript abundance was measured using quantitative PCR (Q-PCR) and expressed relative to that of the housekeeping gene (CmUbiquitin). Grey bars with the same grey letter are not significant different at P<0.05 by Newmans Keuls multiple range test. Dark grey bars with the same letter are not significant different at P<0.05 by Newmans Keuls multiple range test.
Despite the expression of Cm-SPX2 was the highest under NoP from all the studied genes, it showed no positive correlations with phenotypical traits, but had a negative correlation with RSRa in both control and NoP conditions.
Biomass allocation and PUE traits vary significantly between melon accessions
The responses displayed by the nine melon accessions studied were diverse in terms of plant morphology, P uptake and use. Plants were evaluated in an early stage and, therefore, part of the observed variation might be due to differences in the seed size. Nevertheless, previous studies with these accessions have shown that seed weight affects the initial biomass, especially in NoP conditions, but not other parameters . The studied accessions can be divided into three groups; the first group is composed of chi-SC, ma-YP and ca-NC, which were small plants and had low measures of PUE, but high RSRa; the second group is composed of cha-PI, re-Du and in-PS, which had intermediate values for PUE; the third group is composed by mo-kha, flex-Ac and in-Am, which had greater biomass and higher values of PUE.
Phosphate mobilization and re-mobilization
Transcriptional changes under Pi starvation have been demonstrated in various crop plants –, , –. Phosphatases and ribonucleases, are important in the mobilization of the organic P in soil for root absorption and remobilization of organic P , , . Our results demonstrate an increase in the expression of two purple acid phosphatases and a ribonuclease under Pi starvation in melon (Figure 4). PAPs have different roles in P response, some are secreted and some have internal functions , . Both melon transcripts studied, Cm-PAP10.1 and Cm-PAP10.2, showed the highest homology to AtPAP10 (At2g16430.2), but they displayed different responses to Pi starvation and different pattern of correlation with other traits, suggesting different roles in Pi nutrition. The fact that Cm-PAP10.1 was associated with improved biomass accumulation and PUE of accessions in control and not under NoP conditions may suggest that it could be a secreted acid phosphatase. Under NoP conditions, the lack of available Pi or organic sources of P in the hydroponic system would not result in improved acquisition as a consequence of increased expression/secretion of this PAP. In contrast, the correlation between Cm-PAP10.2 expression and PUE, indicate that it could be an internal PAP involved in P efficiency remobilizing Pi under NoP conditions (Table 2). Induction of Cm-RNS1 expression was very intense under NoP conditions (Figure 4C). The orthologue of AtRNS1, encoding a secreted protein up-regulated during Pi starvation, it has been suggested to be involved in Pi mobilization from RNA sources extracellular and intracellular under Pi stress, senescence and wounding  . Our correlation results may support a similar role for the melon orthologue described here, but further analysis of RNS and PAPs in melon must be undertaken to define their role in Pi starvation.
Several genes related to glycolysis increase their expression under Pi starvation, increasing the efficiency of this biochemical pathway in terms of Pi , , . Cm-PPCK is an orthologue of AtPPCK1 (At1g08650.1). In arabidopsis PPCK genes are rapidly induced by Pi starvation leading to increase phosphorylation of phosphenolpyruvate carboxylase (PEPC). PEPC bypasses the pyruvate kinase step in glycolysis, and the phosphorylation of PEPC reduces its sensitivity to malate inhibition . Increases in the concentration of PPCK have also been reported to be involved in induction of synthesis and exudation organic acids. Anthocyanin accumulation is a well-documented response to Pi starvation , and it is suggested to protect plants against oxidative stress . As expected our transcript Cm-transferase, which is an orthologue of the arabidopsis anthocyanin 5-aromatic acetyltransferase gene (At5g39090.1) was upregulated under Pi starvation (Figure 5B). We found differences in the expression of Cm-PPCK and Cm-transferase genes indicating a different level of response among accessions. These responses were just moderately correlated with P use and efficiency parameters.
Alteration membrane lipid composition and metabolism
Under Pi starvation lipid composition of plant membranes changes drastically, decreasing phospholipids and increasing non-phosphorous lipids –. In this experiment, we monitored the expression of putative orthologous genes for UDP-sulfoquinovose synthase/SQD1 (At4g33030.1) and UDP-galactosyltransferase/DGD1 (At3g11670.1). Both genes were upregulated under NoP conditions, and as with other genes monitored here there were differences in the expression levels among accessions, although they were less intense than for other genes (Figure 6). Nevertheless there was a moderate positive correlation among the best performing accessions between PUtE and expression of Cm-SQD1 and Cm-DGD1.
P sensing and transport
SPX domain containing proteins are involved in Pi transport and sensing , . The arabidopsis genome contains 20 genes encoding SPX-domain proteins. Our transcript Cm-SPX2 is orthologue of AtSPX2 (At2g26660.1), At-SPX2 isoform, is targeted to the nucleus and is weakly induced by Pi starvation . However, it is difficult to drawn inferences about the functional equivalence, if any, among the SPX isoforms between melon and arabidopsis. The At-PHO1 gene (At3g23430) encodes one SPX protein that is involved in the regulation of Pi homeostasis through the Pi loading to the xylem . In the case of Cm-SPX2, it the highest level of induction among the genes studied (Figure 7), but no correlations between its expression and phenotypical traits was observed (Table 2). This may indicate a general response of all the accessions studied despite the different levels of expression.
The response of plants to Pi starvation is complex involving many genes. Explaining the diversity of Pi starvation responses for different species accessions using nine genes didn't fully account for the phenotypic diversity observed. However, the expression profiles of nine Pi starvation responsive genes in melon have been demonstrated, as well as their differential response among nine diverse accessions. Accessions with higher measures of PUE, such Flex-Ac, in-Am and mo-kha, also had higher Cm-PAP10.1, Cm-PAP10.2 and Cm-RNS1 expression in NoP than any other accessions, whereas the accessions with lower measures of PUE (chi-SC, ma-YP and ca-NC) had low expression levels for these genes. Therefore higher mobilization and remobilization of P may be a preferential source of diversity in Pi starvation adaptation in melon accessions. The response of other putative Pi responsive genes was also demonstrated to be accession dependent, but they collectively had weaker correlations with measures of biomass accumulation and measures of PUE. Further investigations are required to assess the biochemical impacts of these gene expression changes on their substrates and to characterize additional orthologues of Pi starvation inducible genes for correlations with PUE traits, in the light of new sequence data for melon and related species.
Materials and Methods
Nine melon accessions were used in this study. They belong to 5 botanical varieties of both subspecies; three to ssp. agrestis: two conomon from Japan (chi-SC, and ma-YP), and one momordica from India (mo-kha); and six to ssp. melo: two flexuosus types (cha-PI and flex-Ac), and four varieties of the main commercial groups, reticulatus (re-Du), cantalupensis (ca-NC) and two inodorus, Piel de sapo and Amarillo (In-Ps and In-Am; Table 1). These accessions were selected in a previous study as they represent different responses to P starvation in the species .
Seeds were germinated on germination paper for 6 d and then transferred to 34 L tanks for hydroponic growth. There were two different treatments with three plants per accession grown in each treatment: i) control nutrient solution (C), composed of: 3 mM KNO3, 2 mM Ca(NO3)2•4H2O, 0.5 mM MgSO4•7H2O, 0.5 mM (NH4)H2PO4, 25 µM KCl, 12.5 µM H3BO3, 1 µM MnSO4•H2O, 1 µM ZnSO4•7H2O, 0.25 µM CuSO4•5H2O, 1.3 µM (NH4)6MO7O24•4H2O, and 25 µM Fe-NaEDTA; ii) nutrient solution deficient in Pi (NoP) in which 0.5 mM (NH4)2SO4 was added instead of (NH4)H2PO4 . The experiment was repeated three independent times.
Plants were grown in a glasshouse compartment at Warwick HRI latitude 52°12′31″N, longitude 1°36′06″W, 46 m above sea level with 16 h light, 26°C day and 20°C night and 60% humidity. Twenty-one days after sowing, plants were harvested and fresh weight of roots and shoots was measured (RFW and SFW respectively) and the root to shoot ratio calculated as RFW/SFW (RSRa). The length of the longest root of the plant was measured (RL). Cotyledons were removed and shoots were oven dried for total P quantification, whereas roots were frozen with liquid nitrogen for RNA extraction.
Total P determination
Shoot dry weight (SDW) was recorded after oven-drying at 60°C for 72 h. Shoot samples were digested by the addition of 2 mL nitric acid to 0.3 g dried, ground material and processed in a closed vessel acid digestion microwave (MARSXpress; CEM Corporation, Matthews, NC, USA). Digested samples were diluted with 23 mL of de-ionised water and analysed using inductively-coupled plasma emission spectrometry (ICP-ES; JY Ultima 2, Jobin Yvon Ltd., Stanmore, Middlesex, UK) to determine shoot P concentrations.
Using the shoot dry weight values and the P concentration in the tissues shoot-[P], several measurements of P-use efficiency (PUE) were calculated :
Total amount of P in the shoot:
Shoot-P (mg P plant−1) = [P]* SDW
P-uptake efficiency (PUpE) was calculated as the increase in total P content:
PUpE (mg P) = ([PC]*SDWC)−([PNoP]*SDWNoP)
Phosphorus utilization efficiency (PUtE) was calculated as the increase in yield per unit increase in P content:
PUtE (mg SDW mg−1 P) = (SDWC−SDWNoP)/[([PC]*SDWC)−([PNoP]*SDWNoP)]
Physiological P-use efficiency (PPUE) was calculated as the yield divided by tissue P concentration for either the C or NoP treatment:
PPUE (mg2 SDW mg−1 P) = SDWC/[PC] or SDWNoP/[PNoP]
Where SDW is the shoot dry weight for either control (C), or treatments (NoP) and [P] is the tissue P concentration for either control (C), or P starving treatments (NoP).
RNA extraction and reverse transcription
Total RNA was extracted from frozen root tissue using the TRIzol method as described in . To each sample, 1 mL of TRIzol reagent was added, and total RNA was subsequently extracted according to the manufacturer's instructions (Invitrogen, Paisley, UK), with the following modifications: (i) after homogenization with the TRIzol reagent, the samples were centrifuged to remove any remaining plant material and the supernatant was then transferred to a clean Eppendorf tube, and (ii) to aid precipitation of RNA from the aqueous phase, 0.25 mL of isopropanol and 0.25 mL of 1.2 M NaCl solution containing 0.8 M sodium citrate were added. This procedure precipitated the RNA whilst maintaining the proteoglycans and polysaccharides in a soluble form. Extracted total RNA was then purified using the ‘RNA Cleanup’ protocol for RNeasy columns (Qiagen, Hilden, Germany). RNA yield and purity were determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific Inc, Waltham, MA) and agarose gels.
Reverse transcription was performed on 1 µg of total RNA using the ThermoScript RT-PCR system (Invitrogen, Paisley, UK). The cDNA synthesis reaction was carried out using 0.2 µL random hexamers (50 ng µL−1) and 0.8 µL Oligo(dT)20 primer (50 µM) according to the manufacturer's instructions.
Gene identification and primer design
The expression of selected genes, involved in response to P starvation was analyzed by quantitative PCR (Q-PCR). The melon orthologues of genes that are known to be up- or down-regulated under P starvation in A. thaliana (Table 3) were identified in silico using available melon sequence data (, . BLASTx was used to screen the ICUGI melon EST collection .
Primers for quantitative PCR were designed across exon boundaries of the candidate genes with the Primer3, and hairpins and dimer formation were checked with PrimerSelect (DNASTAR, Inc., Madison, WI). Primers were also designed for six possible housekeeping genes. The stability of the housekeeping genes was evaluated with samples of all accessions and both treatments with the program GeNorm (http://medgen.ugent.be/~jvdesomp/genorm/). The ubiquitin gene (MU45991) was selected as housekeeping gene because of its stability and good dissociation curve. Primers used for amplifying the selected target genes are listed in Table 3.
Gene expression analysis by quantitative RT-PCR and statistical analysis
Analysis of transcript levels of each gene was determined by quantitative PCR using an ABI Prism 7900 HT (Applied Biosystems, Paisley, UK sequence detector and SYBR Green fluorescent dye (Bioline Ltd., London, UK). For each accession and treatment, cDNA samples from three biological replicates used. Intra-assay variation was evaluated by performing all amplification reactions in technical triplicate. Reactions (10 µL volume) were conducted in 384-well plates consisting of 2-ng cDNA sample, 10 µM forward and reverse primer, and 5 µL of 2× SYBR Green PCR master mix (Bioline Ltd). The quantitative PCR reaction conditions were 50°C (2 min) followed by 95°C (10 min) for 1 cycle, then 95°C (15 s) followed by 60°C (1 min) for 40 cycles. This was followed by a dissociation step of 95°C for 15 s, 60°C for 15 s and 95°C for 15 s. The dissociation step was included to generate data for melting curve analysis so that primer dimers or nonspecific products could be detected in the reaction. A control reaction of each sample was included in each plate using 2 ng of total RNA, to assess the presence of genomic contamination and primer dimers and/or primer contamination
The cycle threshold and normalized fluorescence (ΔRn) values were determined for each sample during the quantitative PCR cycling reaction using the ABI Prism sequence detector software (v2.0). The cycle threshold value was calculated using a threshold value set at 0.2. A four point standard curve was constructed from 10-fold dilutions of cDNA for each specific gene and the housekeeping gene. Efficiency was calculated from the slope of the linear correlation between Cp (crossing points) values of each dilution and the logarithm of the corresponding amount of RNA, according to the equation . Since efficiency for all genes ranged within 95–105, the relative expression of target genes was related to the expression of the housekeeping gene (Cm-Ubiquitin) with the equation
Pearson correlations among traits and ANOVA analyses were performed using Statgraphics 5.1 software, Newmans-Keuls multiple Range test was used to separate main effect means when the F-test was significant.
The authors thank the COMAV-UPV, ARS-GRIN-USDA, and IPK-Gatersleben germplasm banks for providing many of the accessions studied in this paper.
Conceived and designed the experiments: AF JH HCB. Performed the experiments: AF HCB RMH. Analyzed the data: AF JH BP. Contributed reagents/materials/analysis tools: JH BP FN. Wrote the paper: AF JH. Critical revision of manuscript: FN.
- 1. Vance CP, Uhde-Stone C, Allan DL (2003) Phosphorus acquisition and use: Critical adaptations by plants for securing a nonrenewable resource. New Phytol 157(3): 423–447.CP VanceC. Uhde-StoneDL Allan2003Phosphorus acquisition and use: Critical adaptations by plants for securing a nonrenewable resource.New Phytol1573423447
- 2. Lynch JP (2011) Root phenes for enhanced soil exploration and phosphorus acquisition: Tools for future crops. Plant Physiol 156(3): 1041–1049.JP Lynch2011Root phenes for enhanced soil exploration and phosphorus acquisition: Tools for future crops.Plant Physiol156310411049
- 3. White PJ, Hammond JP (2009) The sources of phosphorus in the waters of great britain RID C-5860-2008. J Environ Qual 38(1): 13–26.PJ WhiteJP Hammond2009The sources of phosphorus in the waters of great britain RID C-5860-2008.J Environ Qual3811326
- 4. Tiessen H (2008) Phosphorus in the global environment. In: White PJ, Hammond JP, editors. The ecophysiology of plant-phosphorus interactions. Dordrecht: Springer. pp. 1–7.H. Tiessen2008Phosphorus in the global environment.PJ WhiteJP HammondThe ecophysiology of plant-phosphorus interactionsDordrechtSpringer17
- 5. Heffer P, Prud'Homme MPR, Muirheid B, Isherwood KF (2006) Phosphorus fertilisation:Issues and outlook. Anonymous Proceedings. York: International Fertilizer Society. P. HefferMPR Prud'HommeB. MuirheidKF Isherwood2006Phosphorus fertilisation:Issues and outlook.Anonymous ProceedingsYorkInternational Fertilizer Society
- 6. Raven JA (2008) Phosphorus and the future. In: White PJ, Hammond JP, editors. The ecophysiology of plant-phosphorus interactions. Springer. pp. 271–283.JA Raven2008Phosphorus and the future.PJ WhiteJP HammondThe ecophysiology of plant-phosphorus interactionsSpringer271283
- 7. Cordell D, Drangert J, White S (2009) The story of phosphorus: Global food security and food for thought. Global Environmental Change-Human and Policy Dimensions 19(2): 292–305.D. CordellJ. DrangertS. White2009The story of phosphorus: Global food security and food for thought.Global Environmental Change-Human and Policy Dimensions19229230510.1016/j.gloenvcha.2008.10.009. 10.1016/j.gloenvcha.2008.10.009.
- 8. Lynch JP (2007) Roots of the second green revolution. Aust J Bot 55(5): 493–512.JP Lynch2007Roots of the second green revolution.Aust J Bot55549351210.1071/BT06118 ER. 10.1071/BT06118 ER.
- 9. Fang ZY, Shao C, Meng YJ, Wu P, Chen M (2009) Phosphate signaling in arabidopsis and oryza sativa. Plant Science 176(2): 170–180.ZY FangC. ShaoYJ MengP. WuM. Chen2009Phosphate signaling in arabidopsis and oryza sativa.Plant Science176217018010.1016/j.plantsci.2008.09.007 ER. 10.1016/j.plantsci.2008.09.007 ER.
- 10. Hammond JP, Bennett MJ, Bowen HC, Broadley MR, Eastwood DC, et al. (2003) Changes in gene expression in arabidopsis shoots during phosphate starvation and the potential for developing smart plants. Plant Physiol 132(2): 578–596.JP HammondMJ BennettHC BowenMR BroadleyDC Eastwood2003Changes in gene expression in arabidopsis shoots during phosphate starvation and the potential for developing smart plants.Plant Physiol132257859610.1104/pp.103.020941. 10.1104/pp.103.020941.
- 11. Hammond JP, White PJ (2011) Sugar signaling in root responses to low phosphorus availability. Plant Physiol 156(3): 1033–1040.JP HammondPJ White2011Sugar signaling in root responses to low phosphorus availability.Plant Physiol156310331040
- 12. Hammond JP, Mayes S, Bowen HC, Graham NS, Hayden RM, et al. (2011) Regulatory hotspots are associated with plant gene expression under varying soil phosphorus supply in brassica rapa. Plant Physiol 156(3): 1230–1241.JP HammondS. MayesHC BowenNS GrahamRM Hayden2011Regulatory hotspots are associated with plant gene expression under varying soil phosphorus supply in brassica rapa.Plant Physiol156312301241
- 13. Wasaki J, Shinano T, Onishi K, Yonetani R, Yazaki J, et al. (2006) Transcriptomic analysis indicates putative metabolic changes caused by manipulation of phosphorus availability in rice leaves. J Exp Bot 57(9): 2049–2059.J. WasakiT. ShinanoK. OnishiR. YonetaniJ. Yazaki2006Transcriptomic analysis indicates putative metabolic changes caused by manipulation of phosphorus availability in rice leaves.J Exp Bot57920492059
- 14. Uhde-Stone C, Zinn KE, Ramirez-Yanez M, Li AG, Vance CP, et al. (2003) Nylon filter arrays reveal differential gene expression in proteoid roots of white lupin in response to phosphorus deficiency. Plant Physiol 131(3): 1064–1079.C. Uhde-StoneKE ZinnM. Ramirez-YanezAG LiCP Vance2003Nylon filter arrays reveal differential gene expression in proteoid roots of white lupin in response to phosphorus deficiency.Plant Physiol13131064107910.1104/pp.102.016881. 10.1104/pp.102.016881.
- 15. Uhde-Stone C, Gilbert G, Johnson JMF, Litjens R, Zinn KE, et al. (2003) Acclimation of white lupin to phosphorus deficiency involves enhanced expression of genes related to organic acid metabolism. Plant Soil 248(1–2): 99–116.C. Uhde-StoneG. GilbertJMF JohnsonR. LitjensKE Zinn2003Acclimation of white lupin to phosphorus deficiency involves enhanced expression of genes related to organic acid metabolism.Plant Soil2481–299116
- 16. Hernandez G, Ramirez M, Valdes-Lopez O, Tesfaye M, Graham MA, et al. (2007) Phosphorus stress in common bean: Root transcript and metabolic responses. Plant Physiol 144(2): 752–767.G. HernandezM. RamirezO. Valdes-LopezM. TesfayeMA Graham2007Phosphorus stress in common bean: Root transcript and metabolic responses.Plant Physiol144275276710.1104/pp.107.096958. 10.1104/pp.107.096958.
- 17. Calderon-Vazquez C, Ibarra-Laclette E, Caballero-Perez J, Herrera-Estrella L (2008) Transcript profiling of zea mays roots reveals gene responses to phosphate deficiency at the plant- and species-specific levels. J Exp Bot 59(9): 2479–2497.C. Calderon-VazquezE. Ibarra-LacletteJ. Caballero-PerezL. Herrera-Estrella2008Transcript profiling of zea mays roots reveals gene responses to phosphate deficiency at the plant- and species-specific levels.J Exp Bot59924792497
- 18. Wasaki J, Yonetani R, Kuroda S, Shinano T, Yazaki J, et al. (2003) Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots. Plant Cell and Environment 26(9): 1515–1523.J. WasakiR. YonetaniS. KurodaT. ShinanoJ. Yazaki2003Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots.Plant Cell and Environment26915151523
- 19. Bariola PA, Howard CJ, Taylor CB, Verburg MT, Jaglan VD, et al. (1994) The arabidopsis ribonuclease gene Rns1 is tightly controlled in response to phosphate limitation. Plant Journal 6(5): 673–685.PA BariolaCJ HowardCB TaylorMT VerburgVD Jaglan1994The arabidopsis ribonuclease gene Rns1 is tightly controlled in response to phosphate limitation.Plant Journal65673685
- 20. Duff SMG, Sarath G, Plaxton WC (1994) The role of acid-phosphatases in plant phosphorus-metabolism. Physiol Plantarum 90(4): 791–800.SMG DuffG. SarathWC Plaxton1994The role of acid-phosphatases in plant phosphorus-metabolism.Physiol Plantarum904791800
- 21. Hammond JP, White PJ (2008) Sucrose transport in the phloem: Integrating root responses to phosphorus starvation. J Exp Bot 59(1): 93–109.JP HammondPJ White2008Sucrose transport in the phloem: Integrating root responses to phosphorus starvation.J Exp Bot5919310910.1093/jxb/erm221. 10.1093/jxb/erm221.
- 22. Plaxton WC, Tran HT (2011) Metabolic adaptations of phosphate-starved plants. Plant Physiol 156(3): 1006–1015.WC PlaxtonHT Tran2011Metabolic adaptations of phosphate-starved plants.Plant Physiol156310061015
- 23. Essigmann B, Guler S, Narang RA, Linke D, Benning C (1998) Phosphate availability affects the thylakoid lipid composition and the expression of SQD1, a gene required for sulfolipid biosynthesis in arabidopsis thaliana. Proc Natl Acad Sci U S A 95(4): 1950–1955.B. EssigmannS. GulerRA NarangD. LinkeC. Benning1998Phosphate availability affects the thylakoid lipid composition and the expression of SQD1, a gene required for sulfolipid biosynthesis in arabidopsis thaliana.Proc Natl Acad Sci U S A95419501955
- 24. Andersson MX, Stridh MH, Larsson KE, Lijenberg C, Sandelius AS (2003) Phosphate-deficient oat replaces a major portion of the plasma membrane phospholipids with the galactolipid digalactosyldiacylglycerol. FEBS Lett 537(1–3): 128–132.MX AnderssonMH StridhKE LarssonC. LijenbergAS Sandelius2003Phosphate-deficient oat replaces a major portion of the plasma membrane phospholipids with the galactolipid digalactosyldiacylglycerol.FEBS Lett5371–3128132
- 25. Tjellstrom H, Sandelius AS (2008) Lipid asymmetry in root plasma membrane during phosphate limiting conditions. Chem Phys Lipids 154: S30–S30.H. TjellstromAS Sandelius2008Lipid asymmetry in root plasma membrane during phosphate limiting conditions.Chem Phys Lipids154S30S30
- 26. Andersson M, Larsson K, Tjellstrom H, Liljenberg C, Sandelius A (2005) The plasma membrane and the tonoplast as major targets for phospholipid- to-glycolipid replacement and stimulation of phospholipases in the plasma membrane RID E-7958–2010. J Biol Chem 280(30): 27578–27586.M. AnderssonK. LarssonH. TjellstromC. LiljenbergA. Sandelius2005The plasma membrane and the tonoplast as major targets for phospholipid- to-glycolipid replacement and stimulation of phospholipases in the plasma membrane RID E-7958–2010.J Biol Chem280302757827586
- 27. Hammond JP, Broadley MR, White PJ (2004) Genetic responses to phosphorus deficiency. Annals of Botany 94(3): 323–332.JP HammondMR BroadleyPJ White2004Genetic responses to phosphorus deficiency.Annals of Botany94332333210.1093/aob/mch156. 10.1093/aob/mch156.
- 28. Pitrat M (2008) Melon (cucumis melo L.). In: Prohens J, Nuez F, editors. Handbook of Plant Breeding. Vegetables I. New York: Springer. pp. 287–314.M. Pitrat2008Melon (cucumis melo L.).J. ProhensF. NuezHandbook of Plant Breeding. Vegetables INew YorkSpringer287314
- 29. Fita A, Pico B, Nuez F (2006) Implications of the genetics of root structure in melon breeding. J Am Soc Hort Sci 131(3): 372–379.A. FitaB. PicoF. Nuez2006Implications of the genetics of root structure in melon breeding.J Am Soc Hort Sci1313372379
- 30. Fita A, Pico B, Monforte AJ, Nuez F (2008) Genetics of root system architecture using near-isogenic lines of melon. J Am Soc Hort Sci 133(3): 448–458.A. FitaB. PicoAJ MonforteF. Nuez2008Genetics of root system architecture using near-isogenic lines of melon.J Am Soc Hort Sci1333448458
- 31. Fita A, Nuez F, Picó B (2011) Diversity in root architecture and response to P deficiency in seedlings of cucumis melo L. Euphytica 1–17.A. FitaF. NuezB. Picó2011Diversity in root architecture and response to P deficiency in seedlings of cucumis melo L.Euphytica117
- 32. Ding G, Liao Y, Yang M, Zhao Z, Shi L, et al. (2011) Development of gene-based markers from functional arabidopsis thaliana genes involved in phosphorus homeostasis and mapping in brassica napus. Euphytica 181(3): 305–322.G. DingY. LiaoM. YangZ. ZhaoL. Shi2011Development of gene-based markers from functional arabidopsis thaliana genes involved in phosphorus homeostasis and mapping in brassica napus.Euphytica1813305322
- 33. Gonzalez-Ibeas D, Blanca J, Roig C, Gonzalez-To M, Pico B, et al. (2007) MELOGEN: An EST database for melon functional genomics. BMC Genomics 8: 306.D. Gonzalez-IbeasJ. BlancaC. RoigM. Gonzalez-ToB. Pico2007MELOGEN: An EST database for melon functional genomics.BMC Genomics8306
- 34. International Cucurbit Genomics Initiative (ICuGI) (2007) Cucurbit genomics database. International Cucurbit Genomics Initiative (ICuGI)2007Cucurbit genomics database.Available: http://www.icugi.org/ via the Internet. Accessed 2011 Dec 22. Available: http://www.icugi.org/ via the Internet. Accessed 2011 Dec 22.
- 35. Blanca JM, Cañizares J, Ziarsolo P, Esteras C, Mir G, et al. (2011) Melon transcriptome characterization: Simple sequence repeats and single nucleotide polymorphisms discovery for high throughput genotyping across the species. The Plant Genome 4(2): 118–131.JM BlancaJ. CañizaresP. ZiarsoloC. EsterasG. Mir2011Melon transcriptome characterization: Simple sequence repeats and single nucleotide polymorphisms discovery for high throughput genotyping across the species.The Plant Genome4211813110.3835/plantgenome2011.01.0003. 10.3835/plantgenome2011.01.0003.
- 36. Gonzalez VM, Rodriguez-Moreno L, Centeno E, Benjak A, Garcia-Mas J, et al. (2010) Genome-wide BAC-end sequencing of cucumis melo using two BAC libraries. BMC Genomics 11: VM GonzalezL. Rodriguez-MorenoE. CentenoA. BenjakJ. Garcia-Mas2010Genome-wide BAC-end sequencing of cucumis melo using two BAC libraries.BMC Genomics11
- 37. Wu P, Ma LG, Hou XL, Wang MY, Wu YR, et al. (2003) Phosphate starvation triggers distinct alterations of genome expression in arabidopsis roots and leaves. Plant Physiol 132(3): 1260–1271.P. WuLG MaXL HouMY WangYR Wu2003Phosphate starvation triggers distinct alterations of genome expression in arabidopsis roots and leaves.Plant Physiol13231260127110.1104/pp.103.021022. 10.1104/pp.103.021022.
- 38. Misson J, Raghothama KG, Jain A, Jouhet J, Block MA, et al. (2005) A genome-wide transcriptional analysis using arabidopsis thaliana affymetrix gene chips determined plant responses to phosphate deprivation. Proc Natl Acad Sci U S A 102(33): 11934–11939.J. MissonKG RaghothamaA. JainJ. JouhetMA Block2005A genome-wide transcriptional analysis using arabidopsis thaliana affymetrix gene chips determined plant responses to phosphate deprivation.Proc Natl Acad Sci U S A102331193411939
- 39. Graham MA, Ramirez M, Valdes-Lopez O, Lara M, Tesfaye M, et al. (2006) Identification of candidate phosphorus stress induced genes in phaseolus vulgaris through clustering analysis across several plant species. Functional Plant Biology 33(8): 789–797.MA GrahamM. RamirezO. Valdes-LopezM. LaraM. Tesfaye2006Identification of candidate phosphorus stress induced genes in phaseolus vulgaris through clustering analysis across several plant species.Functional Plant Biology33878979710.1071/FP06101. 10.1071/FP06101.
- 40. Morcuende R, Bari R, Gibon Y, Zheng W, Pant BD, et al. (2007) Genome-wide reprogramming of metabolism and regulatory networks of arabidopsis in response to phosphorus. Plant Cell and Environment 30(1): 85–112.R. MorcuendeR. BariY. GibonW. ZhengBD Pant2007Genome-wide reprogramming of metabolism and regulatory networks of arabidopsis in response to phosphorus.Plant Cell and Environment3018511210.1111/j.1365-3040.2006.01608.x. 10.1111/j.1365-3040.2006.01608.x.
- 41. Muller R, Morant M, Jarmer H, Nilsson L, Nielsen TH (2007) Genome-wide analysis of the arabidopsis leaf transcriptome reveals interaction of phosphate and sugar metabolism. Plant Physiol 143(1): 156–171.R. MullerM. MorantH. JarmerL. NilssonTH Nielsen2007Genome-wide analysis of the arabidopsis leaf transcriptome reveals interaction of phosphate and sugar metabolism.Plant Physiol143115617110.1104/pp.106.090167. 10.1104/pp.106.090167.
- 42. Hernandez G, Valdes-Lopez O, Ramirez M, Goffard N, Weiller G, et al. (2009) Global changes in the transcript and metabolic profiles during symbiotic nitrogen fixation in phosphorus-stressed common bean plants. Plant Physiol 151(3): 1221–1238.G. HernandezO. Valdes-LopezM. RamirezN. GoffardG. Weiller2009Global changes in the transcript and metabolic profiles during symbiotic nitrogen fixation in phosphorus-stressed common bean plants.Plant Physiol15131221123810.1104/pp.109.143842. 10.1104/pp.109.143842.
- 43. Zheng L, Huang F, Narsai R, Wu J, Giraud E, et al. (2009) Physiological and transcriptome analysis of iron and phosphorus interaction in rice seedlings. Plant Physiol 151(1): 262–274.L. ZhengF. HuangR. NarsaiJ. WuE. Giraud2009Physiological and transcriptome analysis of iron and phosphorus interaction in rice seedlings.Plant Physiol1511262274
- 44. Li L, Qiu X, Li X, Wang S, Zhang Q, et al. (2010) Transcriptomic analysis of rice responses to low phosphorus stress. Chinese Science Bulletin 55(3): 251–258.L. LiX. QiuX. LiS. WangQ. Zhang2010Transcriptomic analysis of rice responses to low phosphorus stress.Chinese Science Bulletin553251258
- 45. Zhang Q, Wang C, Tian J, Li K, Shou H (2011) Identification of rice purple acid phosphatases related to posphate starvation signalling. Plant Biology 13(1): 7–15.Q. ZhangC. WangJ. TianK. LiH. Shou2011Identification of rice purple acid phosphatases related to posphate starvation signalling.Plant Biology131715
- 46. Chen Z, Nimmo GA, Jenkins GI, Nimmo HG (2007) BHLH32 modulates several biochemical and morphological processes that respond to P-i starvation in arabidopsis. Biochem J 405: 191–198.Z. ChenGA NimmoGI JenkinsHG Nimmo2007BHLH32 modulates several biochemical and morphological processes that respond to P-i starvation in arabidopsis.Biochem J405191198
- 47. Nagata T, Todoriki S, Masumizu T, Suda I, Furuta S, et al. (2003) Levels of active oxygen species are controlled by ascorbic acid and anthocyanin in arabidopsis. J Agric Food Chem 51(10): 2992–2999.T. NagataS. TodorikiT. MasumizuI. SudaS. Furuta2003Levels of active oxygen species are controlled by ascorbic acid and anthocyanin in arabidopsis.J Agric Food Chem511029922999
- 48. Wang Y, Ribot C, Rezzonico E, Poirier Y (2004) Structure and expression profile of the arabidopsis PHO1 gene family indicates a broad role in inorganic phosphate homeostasis. Plant Physiol 135(1): 400–411.Y. WangC. RibotE. RezzonicoY. Poirier2004Structure and expression profile of the arabidopsis PHO1 gene family indicates a broad role in inorganic phosphate homeostasis.Plant Physiol1351400411
- 49. Hamburger D, Rezzonico E, Petetot JMC, Somerville C, Poirier Y (2002) Identification and characterization of the arabidopsis PHO1 gene involved in phosphate loading to the xylem. Plant Cell 14(4): 889–902.D. HamburgerE. RezzonicoJMC PetetotC. SomervilleY. Poirier2002Identification and characterization of the arabidopsis PHO1 gene involved in phosphate loading to the xylem.Plant Cell144889902
- 50. Duan K, Yi KK, Dang L, Huang HJ, Wu W, et al. (2008) Characterization of a sub-family of arabidopsis genes with the SPX domain reveals their diverse functions in plant tolerance to phosphorus starvation. Plant Journal 54(6): 965–975.K. DuanKK YiL. DangHJ HuangW. Wu2008Characterization of a sub-family of arabidopsis genes with the SPX domain reveals their diverse functions in plant tolerance to phosphorus starvation.Plant Journal546965975
- 51. Hammond JP, Broadley MR, White PJ, King GJ, Bowen HC, et al. (2009) Shoot yield drives phosphorus use efficiency in brassica oleracea and correlates with root architecture traits. J Exp Bot 60(7): 1953–1968.JP HammondMR BroadleyPJ WhiteGJ KingHC Bowen2009Shoot yield drives phosphorus use efficiency in brassica oleracea and correlates with root architecture traits.J Exp Bot6071953196810.1093/jxb/erp083. 10.1093/jxb/erp083.
- 52. Munger HM, Robinson RW (1991) Nomenclature of cucumis melo L. Cucurbit Genetics Cooperative Reports 14: 43–44.HM MungerRW Robinson1991Nomenclature of cucumis melo L.Cucurbit Genetics Cooperative Reports144344