Normal aging and neurodegenerative diseases both lead to structural and functional alterations in synapses. Comparison of synapses that are generally similar but respond differently to insults could provide the basis for discovering mechanisms that underlie susceptibility or resistance to damage. Here, we analyzed skeletal neuromuscular junctions (NMJs) in 16 mouse muscles to seek such differences. We find that muscles respond in one of three ways to aging. In some, including most limb and trunk muscles, age-related alterations to NMJs are progressive and extensive during the second postnatal year. NMJs in other muscles, such as extraocular muscles, are strikingly resistant to change. A third set of muscles, including several muscles of facial expression and the external anal sphinter, succumb to aging but not until the third postnatal year. We asked whether susceptible and resistant muscles differed in rostrocaudal or proximodistal position, source of innervation, motor unit size, or fiber type composition. Of these factors, muscle innervation by brainstem motor neurons correlated best with resistance to age-related decline. Finally, we compared synaptic alterations in normally aging muscles to those in a mouse model of amyotrophic lateral sclerosis (ALS). Patterns of resistance and susceptibility were strikingly correlated in the two conditions. Moreover, damage to NMJs in aged muscles correlated with altered expression and distribution of CRMP4a and TDP-43, which are both altered in motor neurons affected by ALS. Together, these results reveal novel structural, regional and molecular parallels between aging and ALS.
Citation: Valdez G, Tapia JC, Lichtman JW, Fox MA, Sanes JR (2012) Shared Resistance to Aging and ALS in Neuromuscular Junctions of Specific Muscles. PLoS ONE 7(4): e34640. https://doi.org/10.1371/journal.pone.0034640
Editor: Thomas H. Gillingwater, University of Edinburgh, United Kingdom
Received: February 1, 2012; Accepted: March 2, 2012; Published: April 2, 2012
Copyright: © 2012 Valdez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grant R01AG032322 from the NIH to J.R.S. and J.W.L. G.V. was supported by a NRSA Postdoctoral Fellowship from NINDS/NIH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Normal aging and age-related neurodegenerative diseases both impair neuronal function. The functional decline observed in aging humans and experimental animals was once thought to reflect, in large part, neuronal death. Over the past decade, however, it has become clear that relatively few neurons die during normal aging ; instead, other cellular pathologies predominate of which synaptic dysfunction appears to be a major contributor , , , , , , , . In contrast, neuronal death is a prominent feature - in fact the defining feature - of neurodegenerative diseases , , , . Nonetheless, even in these disorders, synaptic dysfunction often precedes and in some cases may even lead to neuronal death. Thus, it is important to explore relationships of neurodegenerative diseases to normal aging, not only because age is their major risk factor but also because they involve synaptic dysfunction.
One striking feature of neurodegenerative disease is that each one selectively affects particular neuronal populations, even when the causal genes are broadly expressed. For example, familial forms of Huntington's disease, Parkinson's disease, Alzheimer's disease and amyotrophic lateral sclerosis (ALS), lead predominantly to death of striatial spiny neurons, dopaminergic substantia nigra neurons, entorhinal cortex and hippocampal neurons, and spinal motor neurons respectively, even though the mutated genes in each condition –i.e. huntingtin, synuclein, APP/tau, and superoxide dismutase 1 (SOD1) - are expressed in most neurons , . In some cases, the selectivity of neuronal susceptibility is not only cell-type specific but can differentially affect different classes of the same neuronal type. For example, in familial ALS, motor neurons innervating limb and trunk muscles are severely affected, whereas those innervating extraocular muscles and the anal sphincter muscle are largely spared , .
Selective effects of aging on synaptic subpopulations have been less well studied.
Comparison of affected and spared synapses within the same region or broad type could aid in the identification of factors that confer resistance to age-related change and, by extension, to neurodegenerative diseases. Here, we have focused on skeletal neuromuscular junctions (NMJs) to undertake such a comparison. Their large size and accessibility facilitates detailed examination of their structural integrity by light microscopy, thereby permitting large numbers of synapses to be sampled. In fact, age-related structural changes in NMJs of limb and trunk muscles have been documented in several mammalian species including mice and humans , , , , . We therefore asked whether the rates and patterns of change varied among muscles, and found that they did.
We then asked whether muscles in which NMJs were resistant or susceptible to aging differed systematically in other respects. We considered the rostrocaudal and proximodistal position of the muscle, the myosin heavy chains that its fibers expressed (which define fiber type), the location of the MN pools innervating each muscle, and the size of the motor unit (the number of muscle fibers that each motor neuron innervates). Of these factors, innervation by brainstem motor neurons through cranial nerves correlated best with resistance to age-related synaptic disassembly.
Finally, we compared NMJs in old mice with those in a mouse model of amyotrophic lateral sclerosis (ALS). We report similar patterns of susceptibility and resistance in these two conditions, and extend the structural analysis to document differential effects on levels and distribution of two proteins that have been implicated in the pathogenesis of ALS, CRMP4a and TDP-43 , . Our results reveal novel parallels between normal and pathological aging, and provide starting points for seeking factors that could attenuate or minimize synaptic damage in the two conditions.
NMJs in extraocular muscles are resistant to age-related structural changes
In a previous study, we documented age-related structural alterations in the NMJs of three hind-limb muscles, the tibialis anterior, gastrocnemius, and gracilis . Alterations were qualitatively and quantitatively similar in all three muscles. To begin the present study, we asked whether NMJs in all muscles age in similar ways. We initially compared NMJs in the extensor digitorum longus (EDL) of the hindlimb with those in extraocular muscles (EOMs). We chose EDL because it showed similar age related changes to the hindlimb muscles previously described . In contrast, EOMs have been reported to resist damage brought about by ALS as well as Duchenne's muscular dystrophy , , .
We cut longitudinal sections of EDL and EOM from young adult (3–6 months old unless otherwise noted) and old (24–28 months old unless otherwise noted) transgenic mice in which motor axons were labeled with YFP , . Sections were counterstained with α-bungarotoxin (BTX), which binds specifically to AChRs in the postsynaptic membrane, thereby marking synaptic sites. Nearly 90% of NMJs in the EDL of old mice but only 3% of NMJs in the EDL of young mice (Fig. 1A–B and E–L) exhibited one or more of the following eight features: (1) fragmentation of AChR-rich postsynaptic membrane into small islands; (2) decreased AChR density in some or all of the postsynaptic membrane; (3) retraction of the nerve from the postsynaptic apparatus resulting in partial or (4) complete denervation; (5) sprouts arising from nerve terminals that extended beyond the postsynaptic apparatus; (6) swelling or distension in preterminal axons, within 50 µm from NMJs; (7) thinning of preterminal and/or terminal portions of the axon, termed axonal dystrophy; (8) convergence of 2 or more axons on a single postsynaptic site, leading to multiple innervation. These anatomical changes are similar to those reported previously for tibialis anterior, gastrocnemius and gracilis muscles . NMJs in the EOMs (lateral and medial rectus and inferior and superior oblique), in contrast, were strikingly spared (Fig. 1C–D and E–L). The postsynaptic membrane of most old EOM NMJs was fragmented into small islands, but this topology is also a common feature of young adult EOM NMJs (Figure S1), and is therefore not a sign of aging in this muscle. Values were significantly lower for all 7 other age-related changes in old EOM NMJs than in old EDL (Fig. 1F–L). Thus, NMJs in the EOMs resist age-related structural decline.
Muscles from transgenic mice that expressed YFP in axons (green) were stained with BTX to label AChRs in the postsynaptic membrane (red). A) Young adult extensor digitorum longus (EDL). B) Two year-old EDL. C) Young adult extraocular muscle (EOM). D) Old EOM. Age-related alterations are striking in EDL but subtle in EOM. Scale Bar: 10 µm. Eight previously documented age-related alterations were quantified from images such as those shown in Figure 1. E) Fragmentation of the postsynaptic membrane. F) Decreased AChR density. G) Partial denervation. H) Complete denervation. J) Nerve terminal sprouting. I) Preterminal axonal distension. K) Axonal dystrophy. L) Multiple innervation of a single postsynaptic site. Each bar represents mean ± SEM from at least 3 animals, with at least 100 NMJs counted per animal. *p<0.01 by t-test. Scale bar = 10 µm.
Intermuscular variations in susceptibility to aging
Are EOM NMJs unique in their resistance to age-related changes? To address this issue, we analyzed NMJs in 16 other muscles of young adult and old mice. For 8 of 16 muscles, we quantified all of the features enumerated above (Figure S2 and Figure 2); for the others, we quantified five of these features: postsynaptic fragmentation, decreased AChR density, partial and complete denervation, and nerve terminal sprouting (Table 1).
Incidence of age-related alterations shown in NMJs from sternomastoid, Gracilis, Soleus Diaphragm, Gastrocnemis, Anal Sphincter, EDL and EOM. A) Fragmentation of the postsynaptic membrane. B) Decreased AChR density. C) Partial denervation. D) Complete denervation. E) Nerve terminal sprouting. F) Preterminal axonal distension. G) Axonal dystrophy. H) Multiple innervation of a single postsynaptic site. Values from Fig. 1 are replotted for comparison. Each point represents mean ±SEM from at least 3 animals, with at least 100 NMJs counted per animal. Dashed lines are drawn to emphasize that values for external anal sphincter, frontalis and EOM are generally lower than those from other muscles.
Muscles varied greatly in the incidence of age-related changes in their NMJs. In some muscles, such as the soleus of the lower hindlimb, the gracilis of the upper hindlimb, the diaphragm in the trunk and the sternomastoid in the neck, alterations were similar in frequency to those documented above for EDL (Fig. 2A–H). At the other extreme, muscles such as the external anal sphincter (EAS) and the frontalis were largely spared (Fig. 2A–H).
These differences might reflect absolute differences in susceptibility among muscles or differences in the rates at which defects accumulate. To distinguish between these two possibilities, we examined NMJs in 3 year-old mice. The incidence of affected NMJs changed little in the third year for the EDL, although qualitatively, NMJs appeared more seriously affected in 3 year-old mice than in 2 year-old mice (Fig. 3A, D). Likewise, NMJs in EOMs of 3 year-old mice resembled those in 2 year-old mice, with few structural signs of aging at either time (Fig. 3C, D). In contrast, the structure of NMJs in the frontalis changed dramatically during the third year: they were nearly as youthful in appearance in 2 year-old mice as those in EOMs, but the incidence of structural alterations increased ∼5-fold during the third year, approaching that in the EDL (Fig. 3B, D). Based on these results, we analyzed additional muscles at three years of age including the EAS and other muscles of facial expression (interscutularis, levator auris longus [LAL], and levator labii superioris [LLS]) and an accessory EOM which elevates the eyelid (levator palpebrae surperioris [LPS]). NMJs in the LPS, like those in other EOMs, remained spared at three years of age, whereas those in the EAS, interscutularis, LAL, and LLA accumulated defects over the third year (data not shown). Thus, there may be three groups of muscles with different susceptibility to aging: those highly affected by two years of age (such as EDL), those that succumb during the third year and, therefore, have a delayed response to aging (facial and sphincter muscles) and some that are largely spared (EOMs and LPS).
NMJs from EDL (A), frontalis (B) and EOM (C) of 3 year-old mice. D) Incidence of age-related alterations in NMJs of 3 month-, 2 year-, and 3 year-old mice. Each bar represents mean ± SEM from at least 3 animals, with at least 100 NMJs counted per animal. *p<0.025 by t-test. Scale bar = 10 µm.
Rostrocaudal position and segmental innervation
What could account for intermuscular differences in the susceptibility of NMJs to the age-related decline? Muscles differ in many ways that might be correlated with susceptibility, including their position along the rostrocaudal axis, whether they are innervated by spinal or cranial motor neurons, their fiber type composition, the size of their motor units and the length of the axons supplying them. Indeed, the highly susceptible EDL and the spared EOM differ in all of these respects , , , , . First, we used the data in Table 1 to assess the relationship between the segmental origin of the nerves that innervate each muscle and the incidence of age-related alterations in their NMJs. We used segmental innervation as a metric because it is a correlate of the muscle's somite origin, which is unknown in many cases, and of body position, which is difficult to specify in a single dimension.
In general, NMJs in rostral muscles showed less age related changes than those in caudal muscles (Fig. 4). However, hindlimb and neck muscles are similarly affected by aging even though they are innervated by motor neurons located in caudal and cervical portions of the spinal cord, respectively. Moreover, the EAS appeared spared at two years of age despite being quite caudal. Thus rostrocaudal position per se is not directly related to the incidence of age-related changes. On the other hand, there is a striking difference between muscles innervated by brainstem motor neurons (through cranial nerves) and those innervated by spinal motor neurons: the incidence of age-related changes in NMJs was higher in all muscles innervated through spinal nerves than in muscles innervated by cranial nerves III, IV, VI, and VII. Thus, NMJs formed by brainstem motor neurons are generally refractory to age-related defects.
Fiber type composition
In most skeletal muscles, fibers are divided into 4 categories based primarily on the particular myosin heavy chain (MyHC) isoform they express - type I, IIA, IIX or IIB, ranging from slowest to fastest . Individual muscles contain stereotyped proportions of two or more fiber types. Several studies have shown that fast fibers are more susceptible than slow fibers to atrophy and denervation within aging muscles . It therefore seemed plausible that the degree of severity to age related alterations in some muscles is largely dependent on their fiber type composition. Consistent with this idea, many fibers in the highly resistant EOM express an unusual myosin heavy chain, myh13 , .
We used a panel of isoform-specific antibodies to myosin heavy chains to analyze the fiber type composition in a set of young adult muscles that vary in susceptibility to aging. The frontalis, LAL and interscutularis muscles, which exhibit age-related changes only during the third year have a similar fiber type composition to the EDL, which is highly susceptible to age related changes (Figure 5). In contrast, the soleus, with nearly all slower fibers (>90% Type I and IIA) and the EDL, with predominantly fast fibers (>80% Type IIX and IIB) are similarly susceptible (Figure 5 and Table 1). This data suggest that the overall fiber type composition of muscles does not impart resistance.
A) Cross-sections of soleus, EDL, and interscutularis muscles from young adult mice stained with antibodies specific for myosin Type I, IIA or IIB. Scale Bar: 20 µm. B) Fiber type composition of muscles, determined from micrographs such as those in A. NMJs in both soleus and EDL suffer severe age-related changes but differ in fiber type composition. In contrast, NMJs in frontalis, levator auris longus and intercutularis are largely spared from age-related changes, even though their fiber type composition of these muscles is similar to that of the EDL.
The fiber type composition of fast type muscles is known to change with aging, with fast (Type II) converting to slow (Type I) muscle fibers . The switch to a more oxidative type is believed to allow muscle fibers to resist aging. We therefore asked if a high rate of fiber type switching may account for the special resistance of NMJs in muscles such as the frontalis and interscutularis muscles, which are composed of type IIA, IIB and IIX muscle fibers in young adult animals. We stained 2-year old frontalis, interscutularis and EDL muscles for type I muscle fibers. In contrast to young muscle, aged EDL muscle contains a significant number of Type I fibers (Fig. 6A, Fig. 5A and B). However, fiber type composition did not change significantly in aged frontalis or interscutularis muscles (data not shown). Thus, fiber type switching does not appear to account for the resistance of NMJs in facial muscles, at least during the first two years.
A, B) EDL and Soleus muscles from 2 year-old mice were stained for Type 1 muscle fibers. Type 1 muscle fibers are found in old (A) but not young EDL muscles (see Fig. 5). C, D) NMJs in Type I and Type II fibers exhibit age-related changes in EDL (C) and soleus (D). Each bar represents mean ± SEM. Scale bar = 20 µm.
We also asked whether the severity of age-related changes in NMJs within single muscles might be related to fiber type. Unfortunately, we could label only Type I fibers in the fixed whole mounts that are required to assess NMJ architecture. In the soleus >95% of fibers, however, are either Type I or IIA fibers, so nearly all unlabeled fibers were Type IIA fibers. Using this approach we were surprised to discover that Type I fibers exhibited a higher incidence of age-related structural defects in their NMJs than Type IIA fibers in the soleus muscle (Fig. 6B and D). We also used the EDL to compare the incidence of age-related changes between fibers that converted to Type I with other fiber types. The rate of age-related changes in NMJs was similar in Type I (labeled) and Type II fibers (unlabeled) of old EDL (Fig. 6A, C). Hence, particular fiber types do not appear to be endowed with unique abilities to protect their NMJs from aging.
Motor unit size
If motor neurons have limited capacity to maintain nerve terminals, those that innervate many muscle fibers may be at greater risk of losing terminals as their capacity declines with age than those that innervate few fibers. If true, muscles with small motor units might be more resistant to age-related decline than those with large motor units. To test this idea, we used transgenic mice that express YFP in only a small subset of motor axons (thy1-YFP line H) ,  to reconstruct entire axonal arbors of individual motor axons in muscles that vary in susceptibility to age-related changes. Figure 7A shows one example and Figure 7B summarizes data from all young adult muscles analyzed here and in previous studies from our laboratory , . Consistent with previous reports , EOM motor units are small, averaging 5 fibers. Likewise, the LPS, also spared, has motor units of approximately 5 fibers . Conversely, limb muscles, triangularis and omohyoid, all of which are susceptible, have motor units averaging 14 to 45 muscle fibers (Fig. 7B). However, motor units in the frontalis, interscutularis, LLS and LAL muscles, which are largely spared at 2 years of age, are similar in size to those found in omohyoid and limb muscles. Thus, muscles with the smallest motor units are the most resistant to aging, but small motor units are not required to endow NMJs with resistance to aging. Specifically, motor unit size cannot account for the difference between muscles that are most susceptible to age-related changes and those that exhibit such changes only during the third year.
A) Frontalis muscle of a young adult mouse in a simple motor axon was YFP-positive (green). The muscle was also labeled with BTX (red) to mark all postsynaptic sites. Scale bar: 50 µm. B) Motor unit size determined from muscles such as that in A. Bars indicate mean (±SD) of number of motor units in parentheses. Data on neck muscles (sternomastoid, clavotrapezius, and cleidomastoid) are replotted from Schaefer et al., (2005).
We also examined motor units in old muscles. Motor units have been reported to expand during normal aging likely due to sprouting of surviving motor units to innervate synaptic sites left denervated by death of other motor neurons . Consistent with this view, motor units in the omohyoid were on average 2.4 fold larger in old than in young adult muscles (Fig. 8A–C). In contrast, the size of motor units in EOMs, which are resistant, did not increase significantly with age (Fig. 8D–F). Similarly, motor unit size changed little in several facial muscles that are largely spared, including the frontalis, interscutularis, LAL and LLS muscle (not shown).
Tracings from muscles such as the one shown in Figure 7A. A–D) Motor units from the omohyoid (A, B) and extraocular muscles (C, D) of young adult (A, C) and old (B, D) mice. E, F) Motor unit size in young adult and old omohyoid (E) and extraocular (F) muscles. Each bar represents mean ± SEM from at least 4 motor units per muscle and age. Scale bar = 50 µm.
The ability of neurons to transport material to their terminals decreases with age , . Muscle fibers innervated by long motor axons might be more affected by this decrease than those with short motor axons. Two observations suggested, however, that this relationship is not a robust one. First, we compared neck muscles, such as the omohyoid, to lower limb muscles such as the tibialis anterior and soleus. Neck muscles are innervated by shorter axons than lower limb muscles, but NMJs in neck and limb muscles are similarly affected by aging (Table 1). Second, we compared NMJs in proximal and distal portions of single motor units in old muscles. NMJs within motor units varied substantially in structure with some appearing normal and others being almost completely denervated (Figure S3). However, severely affected NMJs were distributed throughout the motor unit, and were not especially prevalent in proximal or distal axonal branches (Figure 9).
A, B) Branching trees from motor units in young adult (A) and old (B) omohyoid muscles. Color of circles represents the degree of AChR occupancy by nerve terminals (color code, bottom right corner). C) No correlation between AChR occupancy and distance of the nerve terminal from the first branch point of the axonal arbor in either the young adult (r = 0.09) or the aged animal (r = 0.05).
Correlated susceptibility of NMJs to age and ALS
Structural alterations of NMJs have been documented in patients with ALS and in mouse models of ALS that overexpress mutant forms of superoxide dismutase-1 (SOD), known to cause dominant familial ALS in humans , . Mice overexpressing the pathogenic human SOD-G93A show motor symptoms at around 14–16 weeks of age (symptomatic stage) and die at 19 weeks of age (end-stage). Synaptic defects in symptomatic SOD-G93A mice resemble those that afflict NMJs in old mice in several respects . Moreover, two muscles that are spared in old mice, the EOMs and anal sphincter, are resistant in humans with ALS , .
Based on these parallels, we analyzed intermuscular variations in the NMJs of SOD-G93A mice. NMJs in EDL of SOD-G93A mice exhibited the defects described above for NMJs in EDL of old mice: postsynaptic fragmentation, decreased AChR density, partial and complete denervation, terminal sprouting, multiple innervation, and axonal distension and dystrophy (Fig. 10A, C–G). In contrast, NMJs in EOMs of SOD-G93A mice were largely spared (Fig. 10B–G).
A, B) Longitudinal sections from EDL (A) and EOM (B) of SOD-G93A mice transgenic mice. Axons and nerve terminals were stained with antibodies against neurofilament and synaptotagmin-2, (green) and postsynaptic sites were stained with BTX (red). Compare with young adult controls in Figure 1 and Figure S1. Alterations are striking in EDL but subtle in EOM. C) Alter ations quantified from images such as those shown in A–B. Each bar represents mean ± SEM from at least 3 animals, with at least 100 NMJs counted per animal. *p<0.02 by t-test. Scale bar = 10 µm.
We also determined the fractions of synaptic sites that were partially or completely denervated in symptomatic SOD-G93A transgenic mice for each of the muscles previously studied in old mice. Results are summarized in Figure 11A. At the symptomatic stage, most NMJs in all hind limb muscles studied were partially or fully denervated. Most trunk muscles (with the exception of the triangularis sterni) were also denervated although to a lesser extent than hind limb muscles. In contrast, most NMJs in facial muscles remained innervated and thus were refractory to the effects of ALS. Similarly, most NMJs in the anal sphincter muscle were innervated in SOD-G93A symptomatic animals (data not shown). NMJs in EOMs, LPS and frontalis muscles were almost completely spared.
A, B) NMJs in muscles from symptomatic (A) or end-stage (B) SOD-G93A mice listed were scored as fully innervated, partially denervated or fully denervated. Values are shown for 14–16 weeks old symptomatic (A) and 19 weeks old end-stage mice (B). C) NMJs from soleus and tibialis anterior muscles of end stage mice, stained as in Fig. 9. Scale bar = 10 µm. Some NMJs remain partially innervated in soleus, but most NMJs are fully denervated with dispersed postsynaptic structures in tibialis anterior.
We then examined NMJs in mice at the end stage of ALS to determine whether spared muscles eventually succumb (Fig. 11B). Most limb and trunk muscles were almost completely denervated at this stage. Two exceptions were the soleus and triangularis, in which ∼40% and 85% of NMJs, respectively, were still partially or fully innervated. Likewise, NMJs in facial muscles were more affected in endstage than symptomatic ALS. In contrast, the NMJs of the EOMs and LPS muscle remained resistant to disease progression. These results demonstrate that most muscles are similarly affected or spared by aging and ALS (Figure 12).
Expression of CRMP4a and TDP-43 in aging and ALS
The parallels between aged and ALS-afflicted NMJs raised the possibility that similar molecular alterations occur in motor neurons in both conditions. To test this idea, we focused on two proteins recently found to be altered in spinal motor neurons of SOD-G93A mice, collapsin response mediator protein 4a (CRMP4a) and TAR DNA-binding protein 43 (TDP-43) , , , , . In spinal motor neurons of young adult control mice, CRMP4a levels are low and TDP-43 is largely nuclear, whereas in spinal motor neurons of symptomatic SOD-G93A mice, CRMP4a is dramatically increased and TDP-43 forms cytoplasmic aggregates , , . We assessed the levels and distribution of CRMP4a and TDP-43 in spinal and cranial motor neurons of young adult, old and SOD-G93A mice.
As reported previously , CRMP4a is nearly undetectable in motor neurons in lumbar segments of the spinal cord of control mice, but readily detectable in about half of the lumbar motor neurons of symptomatic SOD-G93A mice (Fig. 13A, C, E). Similarly, the fraction of lumbar motor neurons containing CRMP4a increased ∼5-fold in old mice (Fig. 12B, E). Similar results were obtained for motor neurons in cervical spinal cord (data not shown). In contrast, few cranial motor neurons were CRMP4a-positive in young adult wild-type (3 months), old wild-type (2 years) or symptomatic SOD-G93A (19 weeks) mice (Fig. 13D, E). Thus, differences between spinal and cranial motor neurons and similarities between normal aging and ALS extend to CRMP4a expression.
A–D) CRMP4a immunoreactivity of motor neurons in caudal spinal cord (SC) from young adult wild type (A), old wild-type (B) and symptomatic SOD-G93A (C) mice and brainstem (BS) of a symptomatic SOD-G93A mouse (D). Somata are labeled with Neurotrace (A, B, D) or YFP (C). E) Percent of motor neurons immunoreactive for CRMP4a in spinal cord and brainstem of young adult, old, and symptomatic SOD-G93A mice. Each bar represents mean ± SEM. *p<0.015 by t-test. Scale bar = 20 µm.
Localization of TDP-43 within motor neurons was also differentially affected in cranial and spinal motor neurons by aging and ALS. As reported previously , TDP-43 formed cytoplasmic aggregates in the cytoplasm of spinal (lumbar and cervical) motor neurons of symptomatic SOD-G93A mice (Fig. 14B, D). Similar cytoplasmic aggregates of TDP-43 were abundant in spinal motor neurons of old mice (Fig. 14C, D). In addition, whereas levels of TDP-43 were high in all spinal motor neurons of young adult wild type mice (3 months), some motor neurons in SOD-G93A and old mice had barely detectably levels of TDP-43 (Fig. 14D). In contrast, the distribution of TDP-43 in cranial motor neurons from 2-year old and SOD-G93A mice was indistinguishable from that in adults (not shown).
A–D) TDP-43 immunoreactivity of motor neurons in caudal spinal cord from young adult wild type (A), old wild-type (B) and symptomatic SOD-G93A (C) mice. Somata are labeled with Neurotrace (A, B) or YFP (C). D) Percent of motor neurons with strong cytoplasmic immunoreactivity, cytoplasmic aggregates, or barely detectable levels of TDP-43 in spinal cord of young adult, old, and symptomatic SOD-G93A mice. Each bar represents mean ± SEM. Scale bar = 20 µm.
This paper reports two sets of comparisons among NMJs in mouse muscles. First, we compared NMJs within and among 16 muscles of old mice. These comparisons established large intra- and inter-muscular differences in the susceptibility of NMJs to age-related structural alterations. By considering these differences in light of structural and functional features of the muscles and their innervation, we drew conclusions about factors that are likely and unlikely to contribute to differential susceptibility to age-related change. Second, we asked whether the inter-muscular differences in old mice were similar to those in a mouse model of the neurodegenerative disease, ALS. This comparison revealed striking similarities between patterns of susceptibility in these two conditions, strengthening the parallels between normal and pathological aging.
Differences among muscles in old mice
In a previous study, we found that few NMJs in hind-limb muscles of young adult mice but a majority of NMJs in hind-limb muscles of 2 year-old mice exhibit one or more of eight structural features: fragmentation of the postsynaptic apparatus, reduced levels of AChRs, partial denervation, complete denervation, sprouting of terminal axons beyond the postsynaptic apparatus, swelling of the preterminal axon near or at the NMJ, thinning of the axon within the NMJ, and multiple innervation of a single postsynaptic site . Here, we determined the frequency of these age-related features in a larger set of muscles from both 2- and 3-year old mice. Frequency varied over a wide range, but muscles can be generally grouped into three categories. First, NMJs in all hind-limb, trunk and neck muscles examined are highly susceptible to age-related decline. Although some differences among muscles in this category are statistically significant, they are relatively small. Second, NMJs in extraocular and levator palpabrae muscles (EOMs and LPS) are remarkably resistant to the deleterious effects of old age. Even in 3 year old mice -at which time a majority of animals in the cohort had already succumbed- NMJs in these muscles retained their youthful appearance. Third, NMJs in a set of head muscles innervated by cranial nerves, as well as the external anal sphincter, appeared to be resistant to age-related decline at 2 year of age but succumbed over the following year. Thus, these muscles exhibit a delayed response to aging.
Similarities between aging and ALS
The structural defects in hind limb NMJs that accumulate during normal aging resemble those that occur in ALS and in mouse models of ALS , , : in both cases, the postsynaptic membrane becomes fragmented, partially or completely denervated, axons sprout beyond the postsynaptic apparatus, some AChR clusters become faint, and some synaptic sites become multiply innervated. Moreover, NMJs in EOMs are spared in old mice, in humans with ALS and, as shown here, in a mouse model of ALS. These parallels led us to ask whether NMJs in other muscles were similarly susceptible or refractory to disassembly caused by aging and ALS.
The EOM and LPS, as expected, are completely spared in ALS (Fig. 1) , . Even at endstage of the disease, NMJs in the EOM and LPS are fully innervated. The external anal sphincter and facial muscles are also partially spared in ALS as they are in old mice. In symptomatic animals, these muscles show little sign of denervation during the initial phase of the disease. As the disease progresses, however, many of their NMJs become denervated. Hence, many muscles are similarly affected by ALS and aging. Interestingly, a few muscles are more susceptible to age than to ALS. Most strikingly, the triangularis muscle remains innervated in ALS even though it is substantially affected in old mice. The soleus muscle has been reported  to resist ALS, but we find that it is initially as affected as other hind limb muscles. As the disease progress, however, denervation of the soleus NMJs significantly slows compared to other limb muscles. Hence, the triangularis and soleus appear capable of recruiting mechanisms that act to prevent or retard destruction of NMJs.
Possible determinants of differential susceptibility to aging and ALS
Why are some NMJs resistant while others are highly sensitive to the deleterious effects of aging and ALS? Among many possible cellular determinants, several seemed reasonable candidates to test. (1) Previous studies have shown that the muscle fiber type composition changes with aging , suggesting that the extent of the change could affect susceptibility. Likewise, slow muscles are reported to be relatively spared in ALS . (2) In that motor neurons have restricted ability to supply their terminal branches, one might imagine that small motor units would be better nurtured and thus more refractory to resist aging and ALS. (3) Because the rate of axonal transport declines with age and during the progression of ALS , , , NMJs far from motor neuronal somata might lose sustenance before those close to their neuronal somata. Our experiments addressed each of these possibilities but our results supported none of them. Likewise, although we did not directly assess whether patterns of muscle activity confer resistance or susceptibility to insults indirect evidence suggests that they do not. EOMs are unique in that they are able to fire at exceptionally high rates (∼600 Hz) but are also resistant to fatigue . However, the other muscles spared by aging and ALS do not share this specialization: many fibers in the external anal sphincter and LPS are tonically active ,  and muscles of facial expression exhibit phasic activity similar to those of somite-derived muscles.
Of all the possibilities we considered, our data favors only one. Most muscles that resist age or ALS related decline are innervated by motor neurons that reside in the brainstem and extend axons through cranial nerves. This is especially true for oculomotor, abducens and trochlear motor neurons that innervate the EOMs and the LPS muscle, which are completely spared by aging and ALS. Most facial nuclei motor neurons also resist aging and ALS, but the partial resistance of facial muscles suggests that some motor neurons may be susceptible. Interestingly a recent study showed that NMJs in caudal and rostral LAL muscle are differentially susceptible to denervation in a mouse model of spinal muscular atrophy . Our studies, however, combined data from all regions of the sampled muscles, so we cannot directly compare our results to those in Ref. 50.
Of muscles innervated by cranial nerves, one apparent exception is the sternomastoid which is severely affected by aging and ALS, but innervated through cranial nerve XI. However, a recent study showed that this muscle is actually supplied by rostral spinal motor neurons that, unusually, ascend to exit through cranial nerve XI . Hence, our data shows that brainstem motor neurons are, in general, particularly resistant to aging and ALS.
The other notable outlier is the external anal sphincter. The preservation of synapses in this muscle may reflect unusual properties. The EAS is a rhabdosphincter – a specialized muscle sphincter composed of striated skeletal muscle – innervated by neurons that reside in the Onuf's nucleus, a distinct nucleus of neurons in the ventral horn of the sacral spinal cord. Although these neurons appear morphologically similar to alpha motor neurons and innervate striated muscle which is under voluntary control, the identity of these neurons as being somatic or autonomic in nature remains under debate , . For example, motor neurons in Onuf's nucleus are severely affected in Shy Drager Syndrome , a disease that causes the degeneration of autonomic neurons in the brain. Therefore, perhaps these neurons are protected in aging and ALS because their genetic profile more closely resemble neurons in the brain than spinal motor neurons. With these findings in mind, an important future direction is to identify the factors that protect cranial motor neurons from aging and disease.
Molecular differences between afflicted and spared NMJs
The similar patterns of structural change in NMJs of old and SOD-G93A mice suggested that molecular parallels might also exist. To test this possibility, we focused on two proteins that have been implicated in ALS: CRMP-4a and TDP-43. Upregulation and mis-localization of CRMP4a and TDP-43, respectively, have been documented in motor neurons. Furthermore, mutations in TDP-43 lead to ALS and frontotemporal dementia of SOD-G93A mice . We found that CRMP4a is also upregulated and TDP-43 mislocalized in motor neurons of old mice that supply susceptible muscles. In contrast, motor neurons with spared NMJs maintain the normal expression level and distribution of these two molecules, both in old mice and in SOD-G93A mice. These results, therefore, provide evidence that NMJs, in aging and ALS, share molecular as well as structural features.
Materials and Methods
Source of mice
Thy1-XFP transgenic mice were described previously . They were bred and aged in our colony. All motor axons are labeled in line YFP-16 and small subsets of motor axons are labeled in line YFP-H. We also obtained young (4 to 5 month-old) and aged (22 to 28-month-old) C57BL/6 mice from the National Institute of Aging and SOD-G93A transgenic mice from the Jackson Laboratory. All experiments were carried out under NIH guidelines and an animal protocol approved by Harvard University Animal Studies Committee.
Mice were anesthetized with sodium pentobarbital and perfused transcardially with 4% p-formaldehyde in 0.1 M phosphate-buffered saline (PBS; pH 7.4). Muscles were then dissected, post-fixed for 30 min and blocked overnight at 4°C (1% Triton X-100, 4% BSA in PBS). They were then incubated for 1–3 days with neurofilament (1∶500) and synaptotagmin-2 (1∶250) antibodies, washed with PBS for 3 hours and incubated for 24 hours with Alexa 555-α-bungarotoxin (Molecular Probes, Eugene, OR) together with secondary antibodies (Alexa-488 anti-mouse IgG1 and Alexa-647 anti-mouse IgG2A; Molecular Probes). After washing for 3 hours in PBS, muscles were whole-mounted on slides in Vectashield (Vector Labs).
In some cases, muscles were incubated for 2 hr with 5 µg/ml Alexa 555-conjugated α -bungarotoxin following post-fixation and before further processing. To stain muscle sections, muscles were dissected and immersed in 30% sucrose overnight. 30 to 40 µm longitudinal sections were obtained and stained as described above.
Muscle fiber composition was determined by immunostaining 10 µm cross-sections of unfixed muscles. Sections were blocked for 30 minutes at room temperature (0.1% Triton X-100, 4% BSA in PBS). They were immediately incubated with anti-MyHC I [A4840 from Developmental Studies Hybridoma Bank (DSHB) and NCLslow from Leica Microsystems/Novacastra Laboratories], anti-MyHC IIA (2F7 and SC-71 from DSHB), or anti-MyHC IIB (BFF3 from DSHB) for 2 hours. Sections were washed 3 times for 5 minutes and immediately incubated with alexa-conjugated secondary antibodies. After a final wash, sections were mounted and visualized.
Spinal cords and brainstem were dissected, postfixed for 2 hours and immersed in 30% sucrose overnight. 40 µm cryosections were obtained, blocked for 1 hour at room temperature (0.2% Triton X-100, 4% BSA in PBS). They were then stained with antibodies against CRMP4a or TDP-43 in blocking solution overnight, washed 3 times with PBS and stained with alexa-conjugated secondary antibodies. All sections were also stained with Neurotrace to visualize neuronal cell bodies. Sources of antibodies were as follows: anti-neurofilament (smi-312, Covance); anti-synaptotagmin 2 (znp-1), Zebrafish International Resource Center (Eugene, OR); anit-CRMP4a (ab23951, Abcam), anti-TDP-43 (3449S, Cell Signaling).
Maximum intensity projections of confocal stacks obtained from aged (≥24 months) and young adult (2–6 months) junctions were created using Metamorph 6.03 software (Molecular Devices, Sunnyvale, CA). Innervation of AChRs was defined as co-localization of axon terminals (green) with fluorescently labeled AChRs (red). Synaptic occupancy was delineated using Metamorph from the overlapping area between the terminal axon and receptors. Synaptic defects were scored using the definitions in Ref. 19: (a) NMJs were scored as fragmented if they contained 5 or more AChR island and/or a segment of the postsynapse showing severe abnormalities such as small and/or irregularly shaped AChR clusters. (b) Faint AChR clusters were defined as noticeably dimmer postsynaptic sites compared to those in the same confocal plane. (c,d) Full or partial denervated postsynaptic sites were fully or completely unapposed by nerve terminals, represented by YFP or a presynaptic marker. (e) Sprouts were terminal extensions of the nerve >1 µm beyond the border of AChR cluster in any direction. (f) Axonal swellings were anomalous distensions of preterminal portions of the axon. (g) Axonal atrophy was characterized by thinning of the axon by approximately 2 µm compared to the diameter of normal axons. (h) Multiple innervation was scored when two or more preterminal axons were seen to enter a single postsynaptic site. In all cases, we sampled NMJs throughout all regions of a muscle, generally by capturing a set of 20× or 40× images centered on the end-plate band at each of three levels through the z-axis.
To determine motor unit size, entire motor axons and their NMJs were imaged at high resolution as above. Z stacks were flattened (Metamorph) and reconstructions generated using Photoshop CS. The branch order for each motor axon was determined by constructing a complete branching diagram for the entire motor axon as described previously .
Fragmentation of neuromuscular junctions in young adult extraocular muscles. Extraocular muscles from young adult transgenic mice that expressed YFP in axons (green) were stained with BTX to label AChRs (Red). In young EOMs, AChR are often highly fragmented compared to AChR clusters in other muscles (see Figure 1A for a comparison to a young EDL NMJ). Scale bar = 20 µm.
Neuromuscular junctions in young adult and old muscles. Longitudinal sections from transgenic mice that expressed YFP in axons (green) were stained with BTX to label AChRs in the postsynaptic membrane (red). A) Young adult sternomastoid. B) Old sternomastoid. C) Young adult frontalis. D) Old frontalis. Scale bar = 10 µm.
Synapses are differentially affected within an aged motor unit. The figure shows 46 NMJs that comprise one axon's motor unit in a 2 year old mouse. As shown by the color code (bottom, right corner), some junctions are completely innervated (red circles) while the AChRs at other junctions are only partially covered by nerve terminals (purple, yellow, green and blue circles). Numbers in left upper corners represent the junction identity used for branch analysis in Figure 9.
Conceived and designed the experiments: GV JCT MAF JWL JRS. Performed the experiments: GV JCT MAF. Analyzed the data: GV JCT MAF JWL JRS. Contributed reagents/materials/analysis tools: GV JCT MAF JWL JRS. Wrote the paper: GV JRS.
- 1. Burke SN, Barnes CA (2006) Neural plasticity in the ageing brain. Nat Rev Neurosci 7: 30–40.SN BurkeCA Barnes2006Neural plasticity in the ageing brain.Nat Rev Neurosci73040
- 2. Andersen BB, Gundersen HJ, Pakkenberg B (2003) Aging of the human cerebellum: a stereological study. J Comp Neurol 466: 356–365.BB AndersenHJ GundersenB. Pakkenberg2003Aging of the human cerebellum: a stereological study.J Comp Neurol466356365
- 3. Burke SN, Barnes CA (2010) Senescent synapses and hippocampal circuit dynamics. Trends Neurosci 33: 153–161.SN BurkeCA Barnes2010Senescent synapses and hippocampal circuit dynamics.Trends Neurosci33153161
- 4. Duan H, Wearne SL, Rocher AB, Macedo A, Morrison JH, et al. (2003) Age-related dendritic and spine changes in corticocortically projecting neurons in macaque monkeys. Cereb Cortex 13: 950–961.H. DuanSL WearneAB RocherA. MacedoJH Morrison2003Age-related dendritic and spine changes in corticocortically projecting neurons in macaque monkeys.Cereb Cortex13950961
- 5. Hof PR, Morrison JH (2004) The aging brain: morphomolecular senescence of cortical circuits. Trends Neurosci 27: 607–613.PR HofJH Morrison2004The aging brain: morphomolecular senescence of cortical circuits.Trends Neurosci27607613
- 6. Page TL, Einstein M, Duan H, He Y, Flores T, et al. (2002) Morphological alterations in neurons forming corticocortical projections in the neocortex of aged Patas monkeys. Neurosci Lett 317: 37–41.TL PageM. EinsteinH. DuanY. HeT. Flores2002Morphological alterations in neurons forming corticocortical projections in the neocortex of aged Patas monkeys.Neurosci Lett3173741
- 7. Vandervoort AA (2002) Aging of the human neuromuscular system. Muscle Nerve 25: 17–25.AA Vandervoort2002Aging of the human neuromuscular system.Muscle Nerve251725
- 8. von Bohlen und Halbach O, Zacher C, Gass P, Unsicker K (2006) Age-related alterations in hippocampal spines and deficiencies in spatial memory in mice. J Neurosci Res 83: 525–531.O. von Bohlen und HalbachC. ZacherP. GassK. Unsicker2006Age-related alterations in hippocampal spines and deficiencies in spatial memory in mice.J Neurosci Res83525531
- 9. De Vos KJ, Grierson AJ, Ackerley S, Miller CC (2008) Role of axonal transport in neurodegenerative diseases. Annu Rev Neurosci 31: 151–173.KJ De VosAJ GriersonS. AckerleyCC Miller2008Role of axonal transport in neurodegenerative diseases.Annu Rev Neurosci31151173
- 10. Morrison BM, Hof PR, Morrison JH (1998) Determinants of neuronal vulnerability in neurodegenerative diseases. Ann Neurol 44: S32–44.BM MorrisonPR HofJH Morrison1998Determinants of neuronal vulnerability in neurodegenerative diseases.Ann Neurol44S3244
- 11. Saxena S, Caroni P (2011) Selective neuronal vulnerability in neurodegenerative diseases: from stressor thresholds to degeneration. Neuron 71: 35–48.S. SaxenaP. Caroni2011Selective neuronal vulnerability in neurodegenerative diseases: from stressor thresholds to degeneration.Neuron713548
- 12. Mehler MF, Gokhan S (2000) Mechanisms underlying neural cell death in neurodegenerative diseases: alterations of a developmentally-mediated cellular rheostat. Trends Neurosci 23: 599–605.MF MehlerS. Gokhan2000Mechanisms underlying neural cell death in neurodegenerative diseases: alterations of a developmentally-mediated cellular rheostat.Trends Neurosci23599605
- 13. Pardo CA, Xu Z, Borchelt DR, Price DL, Sisodia SS, et al. (1995) Superoxide dismutase is an abundant component in cell bodies, dendrites, and axons of motor neurons and in a subset of other neurons. Proc Natl Acad Sci U S A 92: 954–958.CA PardoZ. XuDR BorcheltDL PriceSS Sisodia1995Superoxide dismutase is an abundant component in cell bodies, dendrites, and axons of motor neurons and in a subset of other neurons.Proc Natl Acad Sci U S A92954958
- 14. Reiner A, Medina L, Figueredo-Cardenas G, Anfinson S (1995) Brainstem motoneuron pools that are selectively resistant in amyotrophic lateral sclerosis are preferentially enriched in parvalbumin: evidence from monkey brainstem for a calcium-mediated mechanism in sporadic ALS. Exp Neurol 131: 239–250.A. ReinerL. MedinaG. Figueredo-CardenasS. Anfinson1995Brainstem motoneuron pools that are selectively resistant in amyotrophic lateral sclerosis are preferentially enriched in parvalbumin: evidence from monkey brainstem for a calcium-mediated mechanism in sporadic ALS.Exp Neurol131239250
- 15. Mosier DR, Siklos L, Appel SH (2000) Resistance of extraocular motoneuron terminals to effects of amyotrophic lateral sclerosis sera. Neurology 54: 252–255.DR MosierL. SiklosSH Appel2000Resistance of extraocular motoneuron terminals to effects of amyotrophic lateral sclerosis sera.Neurology54252255
- 16. Elkerdany MK, Fahim MA (1993) Age changes in neuromuscular junctions of masseter muscle. Anat Rec 237: 291–295.MK ElkerdanyMA Fahim1993Age changes in neuromuscular junctions of masseter muscle.Anat Rec237291295
- 17. Fahim MA, Holley JA, Robbins N (1983) Scanning and light microscopic study of age changes at a neuromuscular junction in the mouse. J Neurocytol 12: 13–25.MA FahimJA HolleyN. Robbins1983Scanning and light microscopic study of age changes at a neuromuscular junction in the mouse.J Neurocytol121325
- 18. Prakash YS, Sieck GC (1998) Age-related remodeling of neuromuscular junctions on type-identified diaphragm fibers. Muscle Nerve 21: 887–895.YS PrakashGC Sieck1998Age-related remodeling of neuromuscular junctions on type-identified diaphragm fibers.Muscle Nerve21887895
- 19. Valdez G, Tapia JC, Kang H, Clemenson GD Jr, Gage FH, et al. (2010) Attenuation of age-related changes in mouse neuromuscular synapses by caloric restriction and exercise. Proc Natl Acad Sci U S A 107: 14863–14868.G. ValdezJC TapiaH. KangGD Clemenson JrFH Gage2010Attenuation of age-related changes in mouse neuromuscular synapses by caloric restriction and exercise.Proc Natl Acad Sci U S A1071486314868
- 20. Duplan L, Bernard N, Casseron W, Dudley K, Thouvenot E, et al. (2010) Collapsin response mediator protein 4a (CRMP4a) is upregulated in motoneurons of mutant SOD1 mice and can trigger motoneuron axonal degeneration and cell death. J Neurosci 30: 785–796.L. DuplanN. BernardW. CasseronK. DudleyE. Thouvenot2010Collapsin response mediator protein 4a (CRMP4a) is upregulated in motoneurons of mutant SOD1 mice and can trigger motoneuron axonal degeneration and cell death.J Neurosci30785796
- 21. Neumann M, Sampathu DM, Kwong LK, Truax AC, Micsenyi MC, et al. (2006) Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science 314: 130–133.M. NeumannDM SampathuLK KwongAC TruaxMC Micsenyi2006Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis.Science314130133
- 22. Kaminski HJ, al-Hakim M, Leigh RJ, Katirji MB, Ruff RL (1992) Extraocular muscles are spared in advanced Duchenne dystrophy. Ann Neurol 32: 586–588.HJ KaminskiM. al-HakimRJ LeighMB KatirjiRL Ruff1992Extraocular muscles are spared in advanced Duchenne dystrophy.Ann Neurol32586588
- 23. Feng G, Mellor RH, Bernstein M, Keller-Peck C, Nguyen QT, et al. (2000) Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28: 41–51.G. FengRH MellorM. BernsteinC. Keller-PeckQT Nguyen2000Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP.Neuron284151
- 24. Sartore S, Mascarello F, Rowlerson A, Gorza L, Ausoni S, et al. (1987) Fibre types in extraocular muscles: a new myosin isoform in the fast fibres. J Muscle Res Cell Motil 8: 161–172.S. SartoreF. MascarelloA. RowlersonL. GorzaS. Ausoni1987Fibre types in extraocular muscles: a new myosin isoform in the fast fibres.J Muscle Res Cell Motil8161172
- 25. Wieczorek DF, Periasamy M, Butler-Browne GS, Whalen RG, Nadal-Ginard B (1985) Co-expression of multiple myosin heavy chain genes, in addition to a tissue-specific one, in extraocular musculature. J Cell Biol 101: 618–629.DF WieczorekM. PeriasamyGS Butler-BrowneRG WhalenB. Nadal-Ginard1985Co-expression of multiple myosin heavy chain genes, in addition to a tissue-specific one, in extraocular musculature.J Cell Biol101618629
- 26. Pette D, Staron RS (2000) Myosin isoforms, muscle fiber types, and transitions. Microsc Res Tech 50: 500–509.D. PetteRS Staron2000Myosin isoforms, muscle fiber types, and transitions.Microsc Res Tech50500509
- 27. Goldberg SJ, Shall MS (1999) Motor units of extraocular muscles: recent findings. Prog Brain Res 123: 221–232.SJ GoldbergMS Shall1999Motor units of extraocular muscles: recent findings.Prog Brain Res123221232
- 28. Porter JD, Burns LA, McMahon EJ (1989) Denervation of primate extraocular muscle. A unique pattern of structural alterations. Invest Ophthalmol Vis Sci 30: 1894–1908.JD PorterLA BurnsEJ McMahon1989Denervation of primate extraocular muscle. A unique pattern of structural alterations.Invest Ophthalmol Vis Sci3018941908
- 29. Lexell J (1995) Human aging, muscle mass, and fiber type composition. J Gerontol A Biol Sci Med Sci 50 Spec No: 11–16.J. Lexell1995Human aging, muscle mass, and fiber type composition.J Gerontol A Biol Sci Med Sci50 Spec No1116
- 30. Briggs MM, Schachat F (2002) The superfast extraocular myosin (MYH13) is localized to the innervation zone in both the global and orbital layers of rabbit extraocular muscle. J Exp Biol 205: 3133–3142.MM BriggsF. Schachat2002The superfast extraocular myosin (MYH13) is localized to the innervation zone in both the global and orbital layers of rabbit extraocular muscle.J Exp Biol20531333142
- 31. Schiaffino S, Reggiani C (2011) Fiber types in mammalian skeletal muscles. Physiol Rev 91: 1447–1531.S. SchiaffinoC. Reggiani2011Fiber types in mammalian skeletal muscles.Physiol Rev9114471531
- 32. Keller-Peck CR, Walsh MK, Gan WB, Feng G, Sanes JR, et al. (2001) Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31: 381–394.CR Keller-PeckMK WalshWB GanG. FengJR Sanes2001Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice.Neuron31381394
- 33. Chakkalakal JV, Nishimune H, Ruas JL, Spiegelman BM, Sanes JR (2010) Retrograde influence of muscle fibers on their innervation revealed by a novel marker for slow motoneurons. Development 137: 3489–3499.JV ChakkalakalH. NishimuneJL RuasBM SpiegelmanJR Sanes2010Retrograde influence of muscle fibers on their innervation revealed by a novel marker for slow motoneurons.Development13734893499
- 34. Schaefer AM, Sanes JR, Lichtman JW (2005) A compensatory subpopulation of motor neurons in a mouse model of amyotrophic lateral sclerosis. J Comp Neurol 490: 209–219.AM SchaeferJR SanesJW Lichtman2005A compensatory subpopulation of motor neurons in a mouse model of amyotrophic lateral sclerosis.J Comp Neurol490209219
- 35. Fox MA, Tapia JC, Kasthuri N, Lichtman JW (2011) Delayed synapse elimination in mouse levator palpebrae superioris muscle. J Comp Neurol 519: 2907–2921.MA FoxJC TapiaN. KasthuriJW Lichtman2011Delayed synapse elimination in mouse levator palpebrae superioris muscle.J Comp Neurol51929072921
- 36. Deschenes MR (2011) Motor Unit and Neuromuscular Junction Remodeling with Aging. Curr Aging Sci. MR Deschenes2011Motor Unit and Neuromuscular Junction Remodeling with Aging.Curr Aging Sci
- 37. Frolkis VV, Tanin SA, Gorban YN (1997) Age-related changes in axonal transport. Exp Gerontol 32: 441–450.VV FrolkisSA TaninYN Gorban1997Age-related changes in axonal transport.Exp Gerontol32441450
- 38. Stokin GB, Goldstein LS (2006) Axonal transport and Alzheimer's disease. Annu Rev Biochem 75: 607–627.GB StokinLS Goldstein2006Axonal transport and Alzheimer's disease.Annu Rev Biochem75607627
- 39. Gurney ME, Pu H, Chiu AY, Dal Canto MC, Polchow CY, et al. (1994) Motor neuron degeneration in mice that express a human Cu,Zn superoxide dismutase mutation. Science 264: 1772–1775.ME GurneyH. PuAY ChiuMC Dal CantoCY Polchow1994Motor neuron degeneration in mice that express a human Cu,Zn superoxide dismutase mutation.Science26417721775
- 40. Rosen DR, Siddique T, Patterson D, Figlewicz DA, Sapp P, et al. (1993) Mutations in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis. Nature 362: 59–62.DR RosenT. SiddiqueD. PattersonDA FiglewiczP. Sapp1993Mutations in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis.Nature3625962
- 41. Mannen T, Iwata M, Toyokura Y, Nagashima K (1982) The Onuf's nucleus and the external anal sphincter muscles in amyotrophic lateral sclerosis and Shy-Drager syndrome. Acta Neuropathol 58: 255–260.T. MannenM. IwataY. ToyokuraK. Nagashima1982The Onuf's nucleus and the external anal sphincter muscles in amyotrophic lateral sclerosis and Shy-Drager syndrome.Acta Neuropathol58255260
- 42. Arai T, Hasegawa M, Akiyama H, Ikeda K, Nonaka T, et al. (2006) TDP-43 is a component of ubiquitin-positive tau-negative inclusions in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Biochem Biophys Res Commun 351: 602–611.T. AraiM. HasegawaH. AkiyamaK. IkedaT. Nonaka2006TDP-43 is a component of ubiquitin-positive tau-negative inclusions in frontotemporal lobar degeneration and amyotrophic lateral sclerosis.Biochem Biophys Res Commun351602611
- 43. Cohen TJ, Lee VM, Trojanowski JQ (2011) TDP-43 functions and pathogenic mechanisms implicated in TDP-43 proteinopathies. Trends Mol Med 17: 659–667.TJ CohenVM LeeJQ Trojanowski2011TDP-43 functions and pathogenic mechanisms implicated in TDP-43 proteinopathies.Trends Mol Med17659667
- 44. Wegorzewska I, Bell S, Cairns NJ, Miller TM, Baloh RH (2009) TDP-43 mutant transgenic mice develop features of ALS and frontotemporal lobar degeneration. Proc Natl Acad Sci U S A 106: 18809–18814.I. WegorzewskaS. BellNJ CairnsTM MillerRH Baloh2009TDP-43 mutant transgenic mice develop features of ALS and frontotemporal lobar degeneration.Proc Natl Acad Sci U S A1061880918814
- 45. Fischer LR, Culver DG, Tennant P, Davis AA, Wang M, et al. (2004) Amyotrophic lateral sclerosis is a distal axonopathy: evidence in mice and man. Exp Neurol 185: 232–240.LR FischerDG CulverP. TennantAA DavisM. Wang2004Amyotrophic lateral sclerosis is a distal axonopathy: evidence in mice and man.Exp Neurol185232240
- 46. Atkin JD, Scott RL, West JM, Lopes E, Quah AK, et al. (2005) Properties of slow- and fast-twitch muscle fibres in a mouse model of amyotrophic lateral sclerosis. Neuromuscul Disord 15: 377–388.JD AtkinRL ScottJM WestE. LopesAK Quah2005Properties of slow- and fast-twitch muscle fibres in a mouse model of amyotrophic lateral sclerosis.Neuromuscul Disord15377388
- 47. Hegedus J, Putman CT, Gordon T (2007) Time course of preferential motor unit loss in the SOD1 G93A mouse model of amyotrophic lateral sclerosis. Neurobiol Dis 28: 154–164.J. HegedusCT PutmanT. Gordon2007Time course of preferential motor unit loss in the SOD1 G93A mouse model of amyotrophic lateral sclerosis.Neurobiol Dis28154164
- 48. Keller EL, Robinson DA (1971) Absence of a stretch reflex in extraocular muscles of the monkey. J Neurophysiol 34: 908–919.EL KellerDA Robinson1971Absence of a stretch reflex in extraocular muscles of the monkey.J Neurophysiol34908919
- 49. Porter JD (2002) Extraocular muscle: cellular adaptations for a diverse functional repertoire. Ann N Y Acad Sci 956: 7–16.JD Porter2002Extraocular muscle: cellular adaptations for a diverse functional repertoire.Ann N Y Acad Sci956716
- 50. Murray LM, Comley LH, Thomson D, Parkinson N, Talbot K, et al. (2008) Selective vulnerability of motor neurons and dissociation of pre- and post-synaptic pathology at the neuromuscular junction in mouse models of spinal muscular atrophy. Hum Mol Genet 17: 949–962.LM MurrayLH ComleyD. ThomsonN. ParkinsonK. Talbot2008Selective vulnerability of motor neurons and dissociation of pre- and post-synaptic pathology at the neuromuscular junction in mouse models of spinal muscular atrophy.Hum Mol Genet17949962
- 51. Ryan S, Blyth P, Duggan N, Wild M, Al-Ali S (2007) Is the cranial accessory nerve really a portion of the accessory nerve? Anatomy of the cranial nerves in the jugular foramen. Anat Sci Int 82: 1–7.S. RyanP. BlythN. DugganM. WildS. Al-Ali2007Is the cranial accessory nerve really a portion of the accessory nerve? Anatomy of the cranial nerves in the jugular foramen.Anat Sci Int8217
- 52. Bergmann M, Volpel M, Kuchelmeister K (1995) Onuf's nucleus is frequently involved in motor neuron disease/amyotrophic lateral sclerosis. J Neurol Sci 129: 141–146.M. BergmannM. VolpelK. Kuchelmeister1995Onuf's nucleus is frequently involved in motor neuron disease/amyotrophic lateral sclerosis.J Neurol Sci129141146
- 53. Kihira T, Yoshida S, Yoshimasu F, Wakayama I, Yase Y (1997) Involvement of Onuf's nucleus in amyotrophic lateral sclerosis. J Neurol Sci 147: 81–88.T. KihiraS. YoshidaF. YoshimasuI. WakayamaY. Yase1997Involvement of Onuf's nucleus in amyotrophic lateral sclerosis.J Neurol Sci1478188