The aim of this study was to investigate factors that may improve the condition of a marginal kidney preserved with a normothermic solution following cardiac death (CD) in a model of rat kidney transplantation (RTx).
Post-euthanasia, Lewis (LEW) donor rats were left for 1 h in a 23°C room. These critical kidney grafts were preserved in University of Wisconsin (UW), lactate Ringer's (LR), or extracellular-trehalose-Kyoto (ETK) solution, followed by intracellular-trehalose-Kyoto (ITK) solution at 4, 23, or 37°C for another 1 h, and finally transplanted into bilaterally nephrectomized LEW recipient rats (n = 4–6). Grafts of rats surviving to day 14 after RTx were evaluated by histopathological examination. The energy activity of these marginal rat kidneys was measured by high-performance liquid chromatography (HPLC; n = 4 per group) and fluorescence intensity assay (n = 6 per group) after preservation with UW or ETK solutions at each temperature. Finally, the transplanted kidney was assessed by an in vivo luciferase imaging system (n = 2).
Using the 1-h normothermic preservation of post-CD kidneys, five out of six recipients in the ETK group survived until 14 days, in contrast to zero out of six in the UW group (p<0.01). Preservation with ITK rather than ETK at 23°C tended to have an inferior effect on recipient survival (p = 0.12). Energy activities of the fresh donor kidneys decreased in a temperature-dependent manner, while those of post-CD kidneys remained at the lower level. ETK was superior to UW in protecting against edema of the post-CD kidneys at the higher temperature. Luminescence intensity of successful grafts recovered within 1 h, while the intensity of grafts of deceased recipients did not change at 1 h post-reperfusion.
Citation: Iwai S, Kikuchi T, Kasahara N, Teratani T, Yokoo T, Sakonju I, et al. (2012) Impact of Normothermic Preservation with Extracellular Type Solution Containing Trehalose on Rat Kidney Grafting from a Cardiac Death Donor. PLoS ONE 7(3): e33157. https://doi.org/10.1371/journal.pone.0033157
Editor: Mercedes Susan Mandell, University of Colorado, United States of America
Received: August 23, 2011; Accepted: February 6, 2012; Published: March 21, 2012
Copyright: © 2012 Iwai et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was funded by Otsuka Pharmaceutical Factory, Inc. (Naruto, Japan, http://www.otsukakj.jp/en/). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: This study was funded by Otsuka Pharmaceutical Factory, Inc. (Naruto, Japan, http://www.otsukakj.jp/en/). EK is a chief scientific advisor at Otsuka Pharmaceutical Factory, Inc.. This not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.
Renal transplantation (RTx) offers prolonged survival and improved quality of life for patients with end-stage renal disease who are facing dialysis . To bridge the growing gap between organ supply and demand, recent efforts have relied on donors considered to be less than optimal, including those who are older, have experienced cardiac death (CD), and those with comorbidities that are potentially detrimental to long-term graft survival –. Despite the increasing use of organs from such marginal donors, waiting lists and deaths while awaiting RTx have been on the rise . Overall, marginal RTx are limited by inferior outcomes and higher costs .
Compared with organs from living and brain-dead donors, kidneys from CD donors (CDD) are significantly more prone to ischemia/reperfusion injury, which may account for the higher rates of both primary non-function and delayed graft function (DGF) , . Indeed, the presence of DGF increases the risk of an acute rejection and doubles the rate of graft loss within 5 years , .
To maintain the function of marginal organs, various preservation methods have been developed and promoted –. Although the use of hypothermic conditions in order to reduce metabolism and oxygen demand has been a fundamental principle in clinical organ preservation , several normothermic preservation and perfusion methods have recently been proposed , –. For instance, a warm flush can be used to achieve more rapid clearance of blood from the microcirculation, whereas a warm preservation solution may reduce vasoconstriction and prevent cell membrane stiffening of both endothelial cells and the cellular components of the blood . These actions may then ameliorate the no-reflow phenomenon, an important mechanism in ischemia/reperfusion injury . Unfortunately, rewarming induces ultrastructural changes in renal tubular cells similar to those observed in acute tubular necrosis, which are associated with renal failure . Thus, further investigations of the effects of normothermic preservation of grafts from marginal donors are clearly warranted.
ET-Kyoto Solution (ETK) was originally developed for use in preserving lung tissue , . ETK contains trehalose and gluconate, two distinct components which have a cytoprotective effect and prevent cells from swelling , . Since ETK has a high-sodium/low-potassium composition akin to extracellular fluid, it prevents the vasoconstriction induced by high potassium concentration and cellular edema , . In addition, ETK has other advantages, including the ability to be stored at room temperature for 3 years . This characteristic of ETK could provide significant cost savings compared with UW.
Genetically encoded biological light probes can be used as high-performance tools to visualize spatiotemporal tissue fate in living animals. In vivo luciferase imaging is obtained from luciferase-expressing LEW transgenic rats via the use of a noninvasive bioimaging system . Due to a close correlation between the two factors, a change in ATP activity can be quantified according to fluorescence intensity .
The purpose of this study was to investigate whether a post-CD kidney could be transplanted following prolonged control of preservation temperatures using ETK in a rat RTx model. The effect of normothermic preservation with ETK was compared to those of preservation with University of Wisconsin (UW) and intracellular-trehalose-Kyoto (ITK) solution.
Survivals after RTx from CD donors
Survival rates of all rats post-RTx from CD donors are summarized in Table 1. In experiment 1, all rats implanted with grafts that were left for 1 h following CD (CD1h group) survived. However, all rats implanted with grafts that were left for 2 h after CD (CD2h group) died within 5 days. Thus, the survival rate of the CD1h group was significantly greater than that of CD2h group (p<0.01). Next, we investigated various methods that could extend the preservation period of CD1h.
In experiment 2, the survival rate of the ETK-preservation group at 23°C (ETK23 group; 12.2±4.0 days) was significantly greater than that of all the other groups (ETK at 4°C [3.7±0.6 days], ETK at 37°C [2.7±0.6 days], LR at 4°C [3.0±1.0 days], LR at 23°C [6.3±5.2 days], and the 2-h ETK preservation at 23°C [3.3±1.3 days]; p<0.01 for all comparisons). Our results suggest normothermic preservation as an optimal method for extending the viability of CD1h.
In experiment 3, we assessed the effect of the preservation temperatures using UW and ITK. Only one recipient survived in response to preservation with UW at 4°C (4.7±4.7 days). However, no recipient survived beyond 5 days post-RTx under normothermic preservation with UW (average, 3±1.1 days). Preservation with ITK at 23°C (ITK23) resulted in a higher survival rate compared to UW at 4°C (p = 0.09) and UW at 23°C (p<0.01). Although ITK23 preservation (9.5±4.3 days) also tended to have an inferior effect on the recipient's survival in comparison to ETK, no significant differences were observed.
Blood assay after RTx
Changes in blood urea nitrogen (BUN) and serum creatinine (Cre) post-RTx for experiments 1, 2, and 3 are shown in Figures 1, 2, and 3, respectively. Regardless of experimental group, levels of BUN and Cre of all surviving rats reached a peak at day 2 and recovered to a nearly normal range by day 14. However, levels of both BUN and Cre of deceased rats tended to increase with elapsed time.
(A): Survival rate of each group. Survival rate of the CD1h group was significantly greater than that of the 2-h post-CD group (* p<0.01 using Log-Rank test.). (B): BUN. (C): Cre. BUN and Cre levels of all surviving rats were gradually recovered to a normal range by day 14 after RTx.
(A): Survival rate of each group. Survival rate of the group receiving kidneys preserved with ET-Kyoto solution (ETK) for 1 h at 23°C (ETK23 group) was significantly longer than that of the other groups (# p<0.01 using Log-Rank test.). (B): BUN and (B'): Cre of the ETK23 group. (C): BUN and (C'): Cre of the other groups without EK23. In all groups in experiment 2, BUN and Cre of all surviving rats reached a peak at day 2 and recovered to a nearly normal range by day 14. (BUN and Cre of the LR23 group was n = 1 after day 4.)
(A): Preservation with ITK at 23°C (ITK23) tended to increase the survival rate compared to UW at 4°C (p = 0.09) and UW at 23°C (p<0.01). (B): BUN and Cre levels of all surviving rats were gradually recovered to a normal range by day 14 after RTx. (BUN and Cre levels of the UW4 group were based on a single animal after day 4.)
Pathological status of kidney graft after RTx
Only kidneys of rats surviving until day 14 were confirmed using azan stain to investigate fibrotic level. Even the graft kidneys of the CD1h group were replaced by fibrosis, suggesting severe tissue injury. The pathological scores of the CD1h alone, ETK23, UW4, and CD1h followed by LR preservation at 23°C for 1 h (LR23) groups were 2.0±0.4, 1.76±0.5, 1.57±0.6, and 2.1±0.5, respectively. The tissue fibrosis of the ETK23 and UW4 groups tended to be inhibited in contrast to that of the other groups, although these differences were not statistically significant (Figure 4). Thus, it remains possible that ETK and UW reduce tissue injury of the marginal kidney.
After euthanasia on day 14 after RTx, kidneys were harvested and stained with azan stain for parenchymal fibrosis. Fibrosis was graded numerically as follows; (A): 0 = none, (B): 1 = minimal, (C): 2 = moderate, and (D): 3 = severe. In each slide, 20 fields were evaluated by four observers blinded to treatment (4×20 fields). Fibrosis of the group receiving kidneys preserved with ETK23 tended to be lower than that of the other groups, but the difference was not significant (p>0.05).
Gravimetric measurements of kidney weights
CD kidney weight was measured to evaluate tissue edema in response to use of ETK and UW at 4, 23 and 37°C (Figure 5). In contrast to ETK preservation at 4°C, the donor kidney weight was significantly lower with ETK at 23°C (p<0.05) and ETK at 37°C (p<0.001). Although tissue edema was also significantly reduced with UW at 23°C (p<0.01) and UW at 37°C (p<0.05) in contrast to UW at 4°C, this effect tended to be less pronounced than with ETK. For instance, at 37°C, the kidney weights of ETK were significantly lower than that of UW (p<0.001).
ETK significantly reduced the kidney weights (KW) with increasing preservation temperature (* ETK at 23°C vs. ETK at 4°C, p<0.05; and ETK at 23°C vs. ETK at 37°C, p<0.001). The prevention of cellular edema with the UW solution was less pronounced as compared to ETK (# UW at 23°C vs. UW at 4°C, p<0.01; and UW at 23°C vs. UW at 37°C, p<0.05). At 37°C, the KW of ETK was significantly lower than that of UW († p<0.001).
Analysis of energy charge in preserved rat kidney
Total adenine nucleotide level (TAN, nmol/mg protein) and energy charge (EC) of kidneys are shown in Figure 6. TAN levels of fresh kidneys at 37°C (fresh37) and CD1h kidneys at all temperatures tended to be lower than that of fresh kidneys at 4°C and 23°C (fresh4 and fresh23, respectively). Specifically, the TAN level of fresh37 kidneys was significantly lower than that of fresh4 kidneys in UW-preservation groups (p<0.05). Additionally, the TAN levels of fresh23 and fresh37 kidneys were significantly lower than that of fresh4 kidneys in ETK-preservation groups (p<0.05 and p<0.01, respectively).
(A): The TAN of fresh kidneys at 37°C and CD1h at all temperatures were lower than that of fresh kidneys at 4°C and 23°C (* p<0.05, fresh37 vs. fresh4 in UW; # p<0.05, fresh37 vs. fresh4 and 23 in ETK). (B) The EC of the fresh37, CD1h4, CD1h23, and CD1h37 groups was not higher than that of the fresh4 and fresh23 groups. The EC of fresh23 with UW preservation was significantly lower than that of fresh4 (* p<0.05).
Using ETK preservation, the EC of fresh37, CD1h4, CD1h23, and CD1h37 kidneys tended to be higher than that of fresh4 and fresh23 kidneys. With UW preservation, the EC of fresh23 kidneys was significantly lower than that of fresh4 kidneys (p<0.05).
Determination of fluorescence intensity of kidney chips
Changes in the fluorescence intensity of kidney chips preserved at 4°C are shown in Figure 7. The fluorescence intensity of the UW group at 6 h after preservation (55.54±17.21%) was significantly higher than that of the ETK group (34.00±37.33%, p<0.05). However, the fluorescence intensity of both groups was approximately equal at 24 h (ETK: 26.17±18.63%, UW: 32.23±21.04%, p>0.05). Finally, fluorescence intensities of ETK and UW were significantly higher than that of saline at almost all measurement time points (p<0.05).
The fluorescence intensity of the UW group at 6 h after preservation was significantly higher than that of the ETK group (* p<0.05). However, the fluorescence intensity of both groups was approximately equal at 24 h. (# p<0.05 vs. ETK group).
Changes in luminescence intensity of graft kidney after RTx using Luc-Tg rat
Similar to that observed for the DC1h donor kidney, the photon flux of rat transplanted fresh kidney began to increase from a low level at 1 h after RTx, and subsequently stabilized after 1 day (Figure 8-A). However, the photon flux of the critical kidney was unchanged and the recipient rat did not survive (Figure 8-B).
In the present study, we investigated whether a post-CD donor kidney can function after various prolonged preservation conditions, including variations in the temperature of the preservation solution. Secondly, we studied whether the energy activity of kidneys after CD or graft kidney after reperfusion during RTx was influenced by the type of preservation solution.
We observed that the survival rate of the ETK group was superior to that of the UW and ITK groups at the normothermic temperature; a finding possibly explained by a difference in sodium/potassium composition in the solutions. Specifically, while ETK has a high-sodium/low-potassium composition similar to that of extracellular fluid, UW has a low-sodium/high-potassium composition similar to that of intracellular fluid (Table 2) . Furthermore, Faure et al. reported that compared to cold preservation using traditional UW solution, a high-sodium version of UW decreased GFR, urinary protein, interstitial fibrosis, and CD4+-cell infiltration in a pig auto-RTx model . These findings support our result of a superior survival rate among ETK versus ITK groups. Therefore, extracellular fluid was better adapted to preserve the marginal kidney compared with intracellular fluid at the normothermic state.
Another key consideration of normothermic preservation is the prevention of cellular edema of the critical organ. According to available evidence, tissue fibrosis appears to be associated with cellular edema , . Interestingly, carbohydrates, such as the trehalose found in ETK and the raffinose found in UW, might inhibit the development of cellular edema. The difference in kidney weight following preservation also has an impact on the cellular edema produced from ischemia/reperfusion injury . In our kidney weight measurement assay, graft weight was increased by preservation with ETK in a temperature-dependent matter. However, UW was less effective in this regard. Trehalose provides a larger monolayer lateral expansion and is substantially superior to raffinose in terms of the stabilization of proteins and nucleic acids , –. Furthermore, the phase transition temperature is 24°C with ETK, but 17°C with raffinose , . Based on evidence from canine and human reports, normothermic perfusion provided life-sustaining function, whereas hypothermic machine perfusion failed , . In the current study, we observed that normothermic temperatures were optimal for preservation with ETK. Additionally, at these temperatures, the ETK and ITK preservation groups exhibited a significantly higher survival rate than the UW preservation group. Thus, it was revealed that normothermic preservation with a trehalose-containing solution was important for maintaining the health of a marginal kidney.
Using an in vitro assay, we showed significantly decreased TAN levels of the CD1h versus fresh kidney. During hypoxia, AMP accumulation and EC is stabilized by a reduction in the TAN pool through a degradation of catalyzed AMP , . While our results were in general consensus with these observations, we found no difference in EC between ETK and UW solution preservation at different temperatures. The EC at which vertebrates can survive is 0.5–0.6 . Thus, since the EC of all groups was similar, we suspected that the EC during preservation is not solely associated with survival after reperfusion of the marginal kidney.
Using the marginal Luc-Tg rat RTx system, we also confirmed the importance of immediately increasing ATP following reperfusion for the survival of recipient rats. This suggests that the key to improving the survival of the marginal kidney is a rapid recovery of EC after reperfusion. The energy state is affected by warm ischemia and subsequent recirculation . Adequate cellular ATP is required for reestablishing ionic homeostasis and supporting ATP-dependent membrane pump function during the critical reperfusion period . Cold preservation results in poor oxygen supply and delayed reduction in the levels of vasoconstrictive arachidonate substrate; actions that result in weak vasodilatation and a delayed recover of EC after reperfusion following cold storage , . While cold temperature causes necrosis, the consequent re-warming can induce apoptosis, which leads to the further development of tissue fibrosis . Salahudeen et al. reported that prolonged cold ischemia of cadaveric grafts is a significant predictor of long-term graft loss . The Na+/K+ ATPase of the kidney is particularly sensitive to ATP, where the activity of proximal tubules is in direct proportion to ATP concentration . Therefore, hypothermia can lead to severe injury of the proximal tubules. Thus, we surmise that the biggest factors influencing survival of the marginal kidney are an early recovery of EC and the prevention of cellular edema using normothermic preservation. Furthermore, we observed that the passive prevention of edema using carbohydrates (trehalose) was more effective than active prevention of edema for purposes of preserving a marginal kidney with energy depletion.
In conclusion, normothermic preservation with ETK, an extracellular-type solution containing trehalose, was beneficial for the short-term storage of a marginal kidney. This approach will be able to extend the preservation period of marginal kidneys or to provide improved function of marginal kidneys for longer periods.
Materials and Methods
Male wild LEW rats weighing between 260 and 310 g werepurchased from Charles River (Breeding Laboratories, Kanagawa, Japan). The animals were housed in a temperature- and humidity-controlled environment with a 12-h light/dark cycle and were provided with standard laboratory chow and water ad libitum. Luc-Tg LEW rats, produced from cross-breeding normal LEW rats (Charles River Laboratories Japan, Inc., Kanagawa, Japan) and Luc-Tg rats, were used for the experiments . Rats were fed and bred at the Center for Experimental Medicine, Jichi Medical University. All experiments were conducted by following established guidelines for animal welfare and were approved by the animal ethics committee of Kitasato University (09-149) and Jichi Medical University (1111).
Post-euthanasia, donor rats were left in the 23°C room for a specified time before the left kidney was harvested. The graft kidneys were transplanted into the recipients according to conditions of preservation solutions and timing as described in Table 1.
In experiment 1, LEW rats were sacrificed, and their left kidneys were harvested after 1 or 2 h following CD. Kidneys that were not preserved with any solutions were immediately transplanted into bilaterally nephrectomized recipients.
In experiments 2 and 3, ETK, ITK, LR (Otsuka Pharmaceutical Factory Inc., Naruto, Japan), and UW (ViaSpan, DuPont Pharmaceuticals, Wilmington, DE, USA), were used as the preservation solutions. Components of each solution are shown in Table 2. For each preservation solution, the grafts of recipients were either placed into refrigerators for storage, left in a room maintained at 23°C, or placed in a constant-temperature bath controlled at 37°C. During the preservation period, anesthesia of recipient rats was induced using isoflurane and was maintained throughout surgery. The donor renal artery was anastomosed to the aorta of the recipient, while the renal vein was connected to the inferior vena cava using a side-to-end anastomosis with a continuous running suture. The ureter was anastomosed end-to-end using interrupted sutures . Both kidneys of recipient rats were nephrectomized at the time of RTx.
Measurement of blood samples and survival
Blood samples were obtained for assessment of BUN and Cre on day 0 and each other day after RTx (AU400, Beckman Coulter, Inc., Brea, CA, USA). Each time, samples were centrifuged for 5 min at 3000 rpm, after which serum was collected. Survival of recipient rats was checked daily, until a maximum of 14 days, at which point rats were sacrificed.
Evaluation of histopathological status
Following euthanasia at 14 days post-RTx, only the kidneys of rats surviving until day 14 were harvested and fixed in 10% formalin, and embedded with paraffin. Two-micron paraffin-embedded sections were stained with azan stain for assessment of parenchymal fibrosis. In accordance with previous studies , , fibrosis was graded numerically as none (0), minimal (1), moderate (2), and severe (3). The score index of fibrosis is shown in Figure 5. All slides were evaluated by four blinded observers, each of whom evaluated 20 fields for each slide (4×20 fields).
Gravimetric measurements of kidney weights
Heparinized rats were euthanized by cervical dislocation, and their kidneys were left inside the body for 1 h. Left and right kidneys were perfused with the respective preservation solution (ETK or UW) at 4°C via the renal artery and kidney weights were measured (control). Subsequently, these kidneys were preserved for 2 h with the same solution at various temperatures (4, 23, 37°C). Kidney weights were measured by precision balance after removal of excess solution. These data were expressed as a percentage of control.
Analysis of energy charge in preserved rat kidney
Fresh kidneys (fresh, n = 4 per group) or kidneys left for 1 h in the body after CD (CD1h, n = 4 per group) were nephrectomized and then preserved with ETK or UW at various temperatures (4, 23, and 37°C). One gram of kidney tissue was added to the chilled 0.5 N perchloric acid (5 mL), and then homogenated and centrifuged. This supernatant was added to 0.5 N triethenolamine 2.0 M K2CO3 before being separated by centrifugation. The control condition included no preservation of the kidney. For measurements of ATP, ADP, and AMP, aliquots of 50 µL were applied to an HPLC system using a 15-cm Inertsil ODS-3 column (GL Science Inc., Tokyo, Japan) at UV260 nm with a mobile phase containing 10 mM KH2PO4 with methanol. The results are expressed as nanomoles per milligram of protein. Total adenine nucleotide (TAN) and energy charge (EC) was calculated based on the following formulas:
Determination of fluorescence intensity in organ chips
Luc-Tg rats (n = 7 per group) were anesthetized with diethyl ether. One milliliter of 20% heparin saline solution was administered via the vein of the bulb of the penis or the inferior vena cava. After perfusion, the rats were exsanguinated, the kidney was removed and blood was rinsed with cold PBS. A kidney chip was made from each kidney and homogeneous organ samples with a diameter of 3 mm were prepared using a tissue slicer.
The organ samples were preserved in 96-well plates at a temperature of 4°C. Each plate was prefilled with 220 µL of the preservation solutions, including ETK, UW, and normal saline solution (Otsuka normal saline, Otsuka Pharmaceutical Factory, Inc.)
Fluorescence intensity of kidney samples after preservation was determined at specified time points using a plate reader (Mithras LB940, Berthold Japan, Tokyo, Japan) as previously described . The plate reader was placed in the cold room, and the fluorescence intensity was measured at 4°C. A total of 20 µL of D-luciferin (2.29 mg/mL) was added to each well using an injector provided with the plate reader. The final concentration of D-luciferin in the well was 0.19 mg/mL, while the detection time was 1 s. The measurement was automatically repeated until the peak was detected, which was regarded as the fluorescence intensity. After the measurement, preservation solutions were immediately replaced.
Detection and quantification of transgene expression using a non-invasive in vivo imaging system
In vivo luciferase imaging was performed using the noninvasive bioimaging system IVIS (Xenogen, Alameda, CA, USA) and IVIS Living Image (Xenogen) software package. The fresh or critical kidneys of Luc-Tg rats were transplanted into wild LEW rats respectively using a previously described rat RTx model. To detect photons from the Luc-Tg rat kidney, D-luciferin (Promega, Madison, WI, USA) was injected into the tail vein of the anesthetized recipient rat (30 mg/kg/body weight, dissolved and diluted to 15 mg/mL in PBS). The signal intensity was quantified as photon flux in units of photons/s/cm2 of a steradian in the region of interest , .
Data are presented as the mean ± SD. Survival curves were determined using the Kaplan-Meier method, while survival statistics were conducted by a Log-Rank test. Repeated measurements of BUN and Cre levels were compared between two groups using a Student's t-test, and among three or six groups using a nonparametric test (Kruskal-Wallis test). TAN, EC, and within-group variance of kidney weights were evaluated by Bartlett's test for equality of variance followed by Dunnett's test. Comparison between groups of different temperatures was performed by an F-test for homogeneity of variance followed by Student's t-test. The fluorescence intensity of kidney samples was tested by one-way analysis of variance (ANOVA) followed by Tukey post-hoc tests. Histopathological scores were evaluated by one-way ANOVA. A P value<0.05 was considered to be statistically significant.
The authors would like to thank Mr. Yoshinaga N and Mr. Arata T for their support of this study. EK is a chief scientific advisor at Otsuka Pharmaceutical Factory, Inc.
Conceived and designed the experiments: SI EK. Performed the experiments: SI TK NK TT TY IS SO. Analyzed the data: SI. Contributed reagents/materials/analysis tools: SI TK TT EK. Wrote the paper: SI. Performed rat renal transplantations and obtained all samples about the rat renal transplantations model: SI. Pathological analysis: TY IS SO. Detection and quantification of transgene expression using a non-invasive in vivo imaging system: SI. Determination of fluorescence intensity of organ samples and measurement of ATP and ADP: TK. Measurement of kidney weight: NK.
- 1. Wolfe RA, Ashby VB, Milford EL, Ojo AO, Ettenger RE, et al. (1999) Comparison of mortality in all patients on dialysis, patients on dialysis awaiting transplantation, and recipients of a first cadaveric transplant. N Engl J Med 341: 1725–1730.RA WolfeVB AshbyEL MilfordAO OjoRE Ettenger1999Comparison of mortality in all patients on dialysis, patients on dialysis awaiting transplantation, and recipients of a first cadaveric transplant.N Engl J Med34117251730
- 2. Reddy SP, Brockmann J, Friend PJ (2009) Normothermic perfusion: A mini-review. Transplantation 87: 631–632.SP ReddyJ. BrockmannPJ Friend2009Normothermic perfusion: A mini-review.Transplantation87631632
- 3. Singh RP, Farney AC, Rogers J, Gravel MT, Chenault RH, et al. (2011) Kidney transplantation from donation after cardiac death donors: lack of impact of delayed function on post-transplant outcomes. Clin Transplant 25: 255–264.RP SinghAC FarneyJ. RogersMT GravelRH Chenault2011Kidney transplantation from donation after cardiac death donors: lack of impact of delayed function on post-transplant outcomes.Clin Transplant25255264
- 4. Welters HH, Brockman JG, Deller R, Suwelackb B, Krieglsteina CF, et al. (2006) Kidney transplantation using donors with history of diabetes and hypertension. Transplant Proc 38: 664–665.HH WeltersJG BrockmanR. DellerB. SuwelackbCF Krieglsteina2006Kidney transplantation using donors with history of diabetes and hypertension.Transplant Proc38664665
- 5. Sung RS, Guidinger MK, Christensen LL, Ashby VB, Merion RM, et al. (2006) Development and current status of ECD kidney transplantation. Clin Transpl 37–55.RS SungMK GuidingerLL ChristensenVB AshbyRM Merion2006Development and current status of ECD kidney transplantation.Clin Transpl3755
- 6. Pomfret EA, Sung RS, Allan J, Kinkhabwala M, Melancon JK, et al. (2008) Solving the organ shortage crisis: the 7th annual American Society of Transplant Surgeon's State-of-the-Art Winter Symposium. Am J Transplant 8: 745–752.EA PomfretRS SungJ. AllanM. KinkhabwalaJK Melancon2008Solving the organ shortage crisis: the 7th annual American Society of Transplant Surgeon's State-of-the-Art Winter Symposium.Am J Transplant8745752
- 7. Renkens JJ, Rouflart MM, Christiaans MH, Van Den Berg-Loonen EM, Van Hooffet JP, et al. (2005) Outcome of non-heart-beating donor kidneys with prolonged delayed graft function after transplantation. Am J Transplant 5: 2704–2709.JJ RenkensMM RouflartMH ChristiaansEM Van Den Berg-LoonenJP Van Hooffet2005Outcome of non-heart-beating donor kidneys with prolonged delayed graft function after transplantation.Am J Transplant527042709
- 8. Perico N, Cattaneo D, Sayegh MH, Remuzzi G (2004) Delayed graft function in kidney transplantation. Lancet 364: 1814–1827.N. PericoD. CattaneoMH SayeghG. Remuzzi2004Delayed graft function in kidney transplantation.Lancet36418141827
- 9. Yarlagadda SG, Coca SG, Formica RN, Poggio ED, Parikh CR (2009) Association between delayed graft function and allograft and patient survival: a systematic review and meta-analysis. Nephrol Dial Transplant 24: 1039–1047.SG YarlagaddaSG CocaRN FormicaED PoggioCR Parikh2009Association between delayed graft function and allograft and patient survival: a systematic review and meta-analysis.Nephrol Dial Transplant2410391047
- 10. Suthard JH, Belzer FO (1995) Organ preservation. Annu Rev Med 46: 235–247.JH SuthardFO Belzer1995Organ preservation.Annu Rev Med46235247
- 11. Navarro AP, Asher J, Sohrabi S, Reddy M, Stamp S, et al. (2009) Peritoneal cooling may provide improved protection for uncontrolled donors after cardiac death: an exploratory porcine study. Am J Transplant 6: 1317–1323.AP NavarroJ. AsherS. SohrabiM. ReddyS. Stamp2009Peritoneal cooling may provide improved protection for uncontrolled donors after cardiac death: an exploratory porcine study.Am J Transplant613171323
- 12. Treckmann J, Moers C, Smits JM, Gallinat A, Maathuis MH, et al. (2011) Machine perfusion versus cold storage for preservation of kidneys from expanded criteria donors after brain death. Transpl Int 24: 548–554.J. TreckmannC. MoersJM SmitsA. GallinatMH Maathuis2011Machine perfusion versus cold storage for preservation of kidneys from expanded criteria donors after brain death.Transpl Int24548554
- 13. Hosgood SA, Barlow AD, Yates PJ, Snoeijs MG, van Heurn EL, et al. (2010) A pilot study assessing the feasibility of a short period of normothermic preservation in an experimental model of non heart beating donor kidneys. J Surg Res 171: 283–290.SA HosgoodAD BarlowPJ YatesMG SnoeijsEL van Heurn2010A pilot study assessing the feasibility of a short period of normothermic preservation in an experimental model of non heart beating donor kidneys.J Surg Res171283290
- 14. Kay MD, Hosgood SA, Harper SJ, Bagul A, Waller HL, et al. (2010) Normothermic versus hypothermic ex vivo flush using a novel phosphate-free preservation solution (AQIX) in porcine kidneys. J Surg Res 171: 275–282.MD KaySA HosgoodSJ HarperA. BagulHL Waller2010Normothermic versus hypothermic ex vivo flush using a novel phosphate-free preservation solution (AQIX) in porcine kidneys.J Surg Res171275282
- 15. Kay MD, Hosgood SA, Harper SJ, Bagul A, Waller HL, et al. (2007) Static normothermic preservation of renal allograft using a novel nonphosphate buffered preservation solution. Transpl Int 20: 88–92.MD KaySA HosgoodSJ HarperA. BagulHL Waller2007Static normothermic preservation of renal allograft using a novel nonphosphate buffered preservation solution.Transpl Int208892
- 16. Hosgood SA, Nicholson ML (2011) First in man renal transplantation after ex vivo normothermic perfusion. Transplantation 92: 735–738.SA HosgoodML Nicholson2011First in man renal transplantation after ex vivo normothermic perfusion.Transplantation92735738
- 17. Tveita T, Johansen K, Lien AH, Myklebust R, Lindal S (2005) Morphologic changes in tubular cells from in situ kidneys following experimental hypothermia and rewarming. APMIS 113: 13–20.T. TveitaK. JohansenAH LienR. MyklebustS. Lindal2005Morphologic changes in tubular cells from in situ kidneys following experimental hypothermia and rewarming.APMIS1131320
- 18. Bando T, Kosaka S, Liu C, Hirai T, Hirata T, et al. (1994) Effects of newly developed solutions containing trehalose on twenty-hour canine lung preservation. J Thorac Cardiovasc Surg 108: 92–98.T. BandoS. KosakaC. LiuT. HiraiT. Hirata1994Effects of newly developed solutions containing trehalose on twenty-hour canine lung preservation.J Thorac Cardiovasc Surg1089298
- 19. Kajiwara N, Taguchi M, Saito H, Nakajima S, Hayashi A, et al. (1996) Evaluation of a new solution containing trehalose for twenty-hour canine lung preservation. Transpl Int 9: 194–200.N. KajiwaraM. TaguchiH. SaitoS. NakajimaA. Hayashi1996Evaluation of a new solution containing trehalose for twenty-hour canine lung preservation.Transpl Int9194200
- 20. Crowe JH, Crowe LM, Chapman D (1984) Preservation of membranes in anhydrobiotic organisms: the role of trehalose. Science 223: 701–703.JH CroweLM CroweD. Chapman1984Preservation of membranes in anhydrobiotic organisms: the role of trehalose.Science223701703
- 21. Belzer FO, Southard JH (1986) Organ preservation and transplantation. Prog Clin Biol Res 224: 291–303.FO BelzerJH Southard1986Organ preservation and transplantation.Prog Clin Biol Res224291303
- 22. Yoshida H, Okuno H, Kamoto H, Habuchi T, Toda Y, et al. (2002) Comparison of the effectiveness of ET-Kyoto with Euro-Collins and University of Wisconsin solution in cold renal storage. Transplantation 74: 1231–1236.H. YoshidaH. OkunoH. KamotoT. HabuchiY. Toda2002Comparison of the effectiveness of ET-Kyoto with Euro-Collins and University of Wisconsin solution in cold renal storage.Transplantation7412311236
- 23. Ramella SG, Hadj-Aissa A, Barbieux A, Steghens JP, Colpart JJ, et al. (1995) Evaluation of a high sodium-low potassium cold-storage solution by the isolated perfused rat kidney technique. Nephrol Dial Transplant 10: 842–846.SG RamellaA. Hadj-AissaA. BarbieuxJP SteghensJJ Colpart1995Evaluation of a high sodium-low potassium cold-storage solution by the isolated perfused rat kidney technique.Nephrol Dial Transplant10842846
- 24. Vajdova K, Graf R, Clavien PA (2002) ATP-supplies in the cold-preserved liver: A long-neglected factor of organ viability. Hepatology 36: 1543–1552.K. VajdovaR. GrafPA Clavien2002ATP-supplies in the cold-preserved liver: A long-neglected factor of organ viability.Hepatology3615431552
- 25. Moen J, Claesson K, Pienaar H, Lindell S, Ploeg RJ, et al. (1989) Preservation of dog liver, kidney, and pancreas using the Belzer-UW solution with a high-sodium and low-potassium content. Transplantation 47: 940–945.J. MoenK. ClaessonH. PienaarS. LindellRJ Ploeg1989Preservation of dog liver, kidney, and pancreas using the Belzer-UW solution with a high-sodium and low-potassium content.Transplantation47940945
- 26. Faure JP, Baumert H, Han Z, Goujon JM, Favreau F, et al. (2003) Evidence for a protective role of trimetazidine during cold ischemia: targeting inflammation and nephron mass. Biochem Pharmacol 66: 2241–2250.JP FaureH. BaumertZ. HanJM GoujonF. Favreau2003Evidence for a protective role of trimetazidine during cold ischemia: targeting inflammation and nephron mass.Biochem Pharmacol6622412250
- 27. Klausner JM, Paterson IS, Goldman G, Kobzik L, Rodzen C, et al. (1989) Postischemic renal injury is mediated by neutrophils and leukotrienes. Am J Physiol 256: F794–802.JM KlausnerIS PatersonG. GoldmanL. KobzikC. Rodzen1989Postischemic renal injury is mediated by neutrophils and leukotrienes.Am J Physiol256F794802
- 28. Jain NK, Roy I (2009) Effect of trehalose on protein structure. Protein Sci 18: 24–36.NK JainI. Roy2009Effect of trehalose on protein structure.Protein Sci182436
- 29. Zhu B, Furuki T, Okuda T, Sakurai M (2007) Natural DNA mixed with trehalose persists in B-form double-stranding even in the dry state. J Phys Chem B 111: 5542–5544.B. ZhuT. FurukiT. OkudaM. Sakurai2007Natural DNA mixed with trehalose persists in B-form double-stranding even in the dry state.J Phys Chem B11155425544
- 30. Fukuse T, Hirata T, Nakamura T, Ueda M, Kawashima M, et al. (1999) Role of saccharides on lung preservation. Transplantation 68: 110–117.T. FukuseT. HirataT. NakamuraM. UedaM. Kawashima1999Role of saccharides on lung preservation.Transplantation68110117
- 31. Crowe JH, Carpenter JF, Crowe LM (1998) The role of vitrification in anhydrobiosis. Annu Rev Physiol 60: 73–103.JH CroweJF CarpenterLM Crowe1998The role of vitrification in anhydrobiosis.Annu Rev Physiol6073103
- 32. Chen T, Fowler A, Toner M (2000) Literature review: supplemented phase diagram of the trehalose-water binary mixture. Cryobiology 40: 277–282.T. ChenA. FowlerM. Toner2000Literature review: supplemented phase diagram of the trehalose-water binary mixture.Cryobiology40277282
- 33. Brasile L, Stubenitsky BM, Maurits H, Lindell S, Araneda D, et al. (2002) Overcoming severe renal ischemia: the role of ex vivo warm perfusion. Transplantation 73: 897–901.L. BrasileBM StubenitskyH. MauritsS. LindellD. Araneda2002Overcoming severe renal ischemia: the role of ex vivo warm perfusion.Transplantation73897901
- 34. Urbaitis KB (1984) Effect of ischemia and hypertonic saline loading on renal adenine nucleotides. Ren Physiol 7: 22–31.KB Urbaitis1984Effect of ischemia and hypertonic saline loading on renal adenine nucleotides.Ren Physiol72231
- 35. Atokinson DE, Walton GM (1967) Adenosine triphosphate conservation in metabolic regulation. Rat liver citrate cleavage enzyme. J Biol Chem 242: 3239–3241.DE AtokinsonGM Walton1967Adenosine triphosphate conservation in metabolic regulation. Rat liver citrate cleavage enzyme.J Biol Chem24232393241
- 36. Luque P, Alvarez-Vijande R, Alcaraz A, Aguilar A, Alcoberro J, et al. (1999) Experimental study of the cellular energy charge in pig kidney transplants from non-heart-beating donors. Transplant Proc 31: 2352–2353.P. LuqueR. Alvarez-VijandeA. AlcarazA. AguilarJ. Alcoberro1999Experimental study of the cellular energy charge in pig kidney transplants from non-heart-beating donors.Transplant Proc3123522353
- 37. McAnulty JF (1998) The effect of calcium on hypothermia-facilitated resuscitation of warm ischemic kidney tissue slices: a role for the mitochondrial permeability transition pore? Cryobiology 36: 12–19.JF McAnulty1998The effect of calcium on hypothermia-facilitated resuscitation of warm ischemic kidney tissue slices: a role for the mitochondrial permeability transition pore?Cryobiology361219
- 38. Schilling M, Saunder A, Ametani M, Southard JH, Belzer FO (1993) Prolonged kidney preservation by inhibition of arachidonic acid metabolism. Transplant Proc 25: 1629–1630.M. SchillingA. SaunderM. AmetaniJH SouthardFO Belzer1993Prolonged kidney preservation by inhibition of arachidonic acid metabolism.Transplant Proc2516291630
- 39. Salahudeen AK, Huang H, Joshi M, Moore NA, Jenkins JK (2003) Involvement of the mitochondrial pathway in cold storage and rewarming-associated apoptosis of human renal proximal tubular cells. Am J Transplant 3: 273–280.AK SalahudeenH. HuangM. JoshiNA MooreJK Jenkins2003Involvement of the mitochondrial pathway in cold storage and rewarming-associated apoptosis of human renal proximal tubular cells.Am J Transplant3273280
- 40. Salahudeen AK, Haider N, May W (2004) Cold ischemia and the reduced long-term survival of cadaveric renal allografts. Kidney Int 65: 713–718.AK SalahudeenN. HaiderW. May2004Cold ischemia and the reduced long-term survival of cadaveric renal allografts.Kidney Int65713718
- 41. Clausen T, Van Hardeveld C, Everts ME (1991) Significance of cation transport in control of energy metabolism and thermogenesis. Physiol Rev 71: 733–774.T. ClausenC. Van HardeveldME Everts1991Significance of cation transport in control of energy metabolism and thermogenesis.Physiol Rev71733774
- 42. Hakamata Y, Murakami T, Kobayashi E (2006) “Firefly rats” as an organ/cellular source for long-term in vivo bioluminescent imaging. Transplantation 81: 1179–1184.Y. HakamataT. MurakamiE. Kobayashi2006“Firefly rats” as an organ/cellular source for long-term in vivo bioluminescent imaging.Transplantation8111791184
- 43. Fujishiro J, Kudou S, Iwai S, Takahashi M, Hakamata Y, et al. (2006) Use of sphingosine-1-phosphate 1 receptor agonist, KRP-203, in combination with a subtherapeutic dose of cyclosporine A for rat renal transplantation. Transplantation 82: 804–812.J. FujishiroS. KudouS. IwaiM. TakahashiY. Hakamata2006Use of sphingosine-1-phosphate 1 receptor agonist, KRP-203, in combination with a subtherapeutic dose of cyclosporine A for rat renal transplantation.Transplantation82804812
- 44. Takeda S, Takahashi M, Sado Y, Takeuchi K, Hakamata Y, et al. (2004) Prevention of glomerular crescent formation in glomerulonephritis by mycophenolate mofetil in rats. Nephrol Dial Transplant 19: 2228–2236.S. TakedaM. TakahashiY. SadoK. TakeuchiY. Hakamata2004Prevention of glomerular crescent formation in glomerulonephritis by mycophenolate mofetil in rats.Nephrol Dial Transplant1922282236
- 45. Kunter U, Rong S, Boor P, Eitner F, Müller-Newen G, et al. (2007) Mesenchymal stem cells prevent progressive experimental renal failure but maldifferentiate into glomerular adipocytes. J Am Soc Nephrol 18: 1754–1764.U. KunterS. RongP. BoorF. EitnerG. Müller-Newen2007Mesenchymal stem cells prevent progressive experimental renal failure but maldifferentiate into glomerular adipocytes.J Am Soc Nephrol1817541764
- 46. Negishi K, Teratani T, Iwasaki J, Kanazawa H, Kasahara N, et al. (2011) Luminescence technology in preservation and transplantation for rat islet. Islets 3: 111–117.K. NegishiT. TerataniJ. IwasakiH. KanazawaN. Kasahara2011Luminescence technology in preservation and transplantation for rat islet.Islets3111117