Low muscle mass and function have been associated with poorer indicators of physical capability in older people, which are in-turn associated with increased mortality rates. The growth hormone/insulin-like growth factor (GH/IGF) axis is involved in muscle function and genetic variants in genes in the axis may influence measures of physical capability.
As part of the Healthy Ageing across the Life Course (HALCyon) programme, men and women from seven UK cohorts aged between 52 and 90 years old were genotyped for six polymorphisms: rs35767 (IGF1), rs7127900 (IGF2), rs2854744 (IGFBP3), rs2943641 (IRS1), rs2665802 (GH1) and the exon-3 deletion of GHR. The polymorphisms have previously been robustly associated with age-related traits or are potentially functional. Meta-analysis was used to pool within-study genotypic effects of the associations between the polymorphisms and four measures of physical capability: grip strength, timed walk or get up and go, chair rises and standing balance.
Few important associations were observed among the several tests. We found evidence that rs2665802 in GH1 was associated with inability to balance for 5 s (pooled odds ratio per minor allele = 0.90, 95% CI: 0.82–0.98, p-value = 0.01, n = 10,748), after adjusting for age and sex. We found no evidence for other associations between the polymorphisms and physical capability traits.
Our findings do not provide evidence for a substantial influence of these common polymorphisms in the GH/IGF axis on objectively measured physical capability levels in older adults.
Citation: Alfred T, Ben-Shlomo Y, Cooper R, Hardy R, Cooper C, Deary IJ, et al. (2012) A Multi-Cohort Study of Polymorphisms in the GH/IGF Axis and Physical Capability: The HALCyon Programme. PLoS ONE 7(1): e29883. doi:10.1371/journal.pone.0029883
Editor: Raya Khanin, Memorial Sloan Kettering Cancer Center, United States of America
Received: July 25, 2011; Accepted: December 6, 2011; Published: January 10, 2012
Copyright: © 2012 Alfred et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The authors acknowledge the support of Medical Research Council (MRC) and Arthritis Research (United Kingdom). Boyd Orr Funding: The Boyd Orr DNA bank was funded by the Wellcome Trust (Grant number: GR068468MA). Follow-up of the Boyd Orr cohort was supported by grants from the Wellcome Trust, World Cancer Research Fund, Research into Ageing and the British Heart Foundation. The Caerphilly Prospective study was funded by the MRC of the United Kingdom. Samples from the English Longitudinal Study of Ageing DNA Repository, received support under a grant (AG1764406S1) awarded by the National Institute on Ageing (NIA). The Hertfordshire Cohort Study and the Hertfordshire Ageing Study were funded by the MRC and the University of Southampton. Lothian Birth Cohort 1921: The Biotechnology and Biological Sciences Research Council (BBSRC) funded the phenotypic data collection and DNA preparation (project grant 15/SAG09977) and genome-wide association study (project grant BB/F019394/1). The work was undertaken by The University of Edinburgh Centre for Cognitive Ageing and Cognitive Epidemiology, part of the cross council Lifelong Health a'd Wellbeing Initiative (Centre grant G0700704/84698). Funding from the BBSRC, Engineering and Physical Sciences Research Council, Economic and Social Research Council (ESRC) and MRC is gratefully acknowledged. The MRC National Survey of Health and development is funded by the United Kingdom MRC. HALCyon is funded by the New Dynamics of Ageing cross council research programme. DG is a National Institute of Health Research Senior Investigator. RC receives support from the HALCyon programme funded by the New Dynamics of Ageing (RES-353-25-0001). DK and RH are supported by the United Kingdom MRC. MK is supported by NIA, National Institutes of Health (AG1764406S1). TA is an ESRC PhD student. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Muscle mass declines with age ,  and low muscle mass and function in older people have been associated with a range of adverse outcomes , including poorer mobility , increased disability ,  and higher mortality rates . Low muscle mass and area are also independently associated with lower levels of objective measures of physical capability, the capacity to undertake the physical tasks of daily living, including grip strength , ability to balance  and to complete five chair stands . Therefore, understanding the contributors to the inter-individual variability of muscle properties is important and may be relevant to the prevention of the adverse consequences of lower levels of physical capability –. Variability attributed to the growth hormone/insulin-like growth factor (GH/IGF) axis may also have the potential to lead to the development of novel interventions .
The GH/IGF axis plays an important role in body growth and composition . IGF-I is produced in the liver in response to GH, as well as in skeletal muscle, and plays an important role in muscle growth and function –, promoting and differentiating muscle cells, together with IGF-II . Of the six binding proteins, most of IGF-I is bound to IGFBP-3 , which regulates the bioactivity of IGF-I and is expressed in skeletal muscle , possibly having IGF-I independent effects. Insulin receptor substrate 1 (IRS-1) interacts with the IGF receptor  and is involved in regulating body size . The GH receptor is expressed throughout the body  and knockout of the GHR gene in mice leads to a reduction in body and muscle mass . In addition to muscle function, the GH/IGF axis is also believed to play a role in some cancers , , longevity and ageing –. Whilst evidence from small to moderately sized investigations into the effects of the GH/IGF axis on muscle properties and physical performance in humans has been mixed , , it has been hypothesised that polymorphisms in genes in the GH/IGF axis may contribute to measures of muscle functioning and physical capability, phenotypes that have been shown to be partly heritable , .
In genome-wide association studies (GWAS), single-nucleotide polymorphisms (SNPs) around IGF1 have been associated with height – and the C allele of rs35767 upstream of the gene has been associated with increased fasting insulin and insulin resistance risk  as well as lower levels of IGF-I –. There is some evidence to suggest that SNPs in IGF2 are associated with longevity , measures of adult body size –, grip strength , arm and leg strength , and post-exercise muscle damage , and in a GWAS the A allele of rs7127900 near the gene has been associated with increased risk of prostate cancer . The A allele of functional SNP rs2854744 in the promoter region of IGFBP3 has been associated with higher levels of IGFBP-3 ,  and may be associated with reduced breast and prostate cancer risk . Increased type 2 diabetes risk has been associated with the C allele of rs2943641 near IRS1 in GWAS . Studies of the functional SNP rs2665802 (in GH1)  have found associations between its minor allele and lower colorectal cancer risk  and mortality risk in females . The exon-3 deletion of GHR (d3GHR) has been associated with response to GH administration in short children  and is perfectly tagged by several SNPs in Europeans , among which rs6873545 has a suggested association with lung cancer . We therefore examined the associations between these polymorphisms and anthropometric and physical capability traits in 13,364 men and women aged between 52 and 90 years as part of the HALCyon (Healthy Ageing across the Life Course; www.halcyon.ac.uk) programme, in what we believe to be the largest investigation into polymorphisms in the GH/IGF axis and physical capability.
Written informed consent was obtained from all participants. Ethical approval for each study was obtained from the relevant ethics committees.
The Medical Research Council National Survey of Health and Development (NSHD) comprises participants sampled from all births in a week in March 1946 and followed up since. In 1999, at age 53 years, men and women were visited by a research nurse and consent for DNA extraction was given by approximately 2900 members of the cohort. Details of the data collected and the several phases of the study are available on the cohort's website (www.nshd.mrc.ac.uk) and elsewhere .
The English Longitudinal Study of Ageing (ELSA) comprises men and women aged 50 years and over who originally participated in the Health Survey for England in 1998, 1999 or 2001. Fieldwork began in 2002–03 (Phase I) with two-yearly follow-ups and in 2004–05 (Phase II) blood samples were provided by 6231 participants. Details of the cohort have been published .
The Hertfordshire Cohort Study (HCS) consists of 2997 participants born 1931–39 and registered with a General Practitioner in East, North or West Hertfordshire who attended a clinic in 1994–2004 (Phase I). A second assessment took place in 2004–05 for participants in East Hertfordshire (Phase II). Further details of study design, data collected and summaries of participant characteristics have been published  and are available on its website (www.mrc.soton.ac.uk/herts).
The Hertfordshire Ageing Study (HAS) comprises men and women traced in 1994–95, the first follow-up (Phase I), of singleton births from 1920–30 in North Hertfordshire. A total of 717 participants attended a clinic during which DNA was extracted. A second follow-up took place in 2003–05 (Phase II). Details of the recruitment, data collected and summaries of participant characteristics have been described previously .
The Boyd Orr cohort is a historical cohort study based on children surveyed in 1937–39 in English and Scottish districts. Participants were followed-up for vital status via the NHS Medical Information Research Service (MIRS) since 1948, with questionnaire administration to survivors in 1997–98 (Phase II) and a research clinic visit in 2002–03 (Phase III), during which DNA was extracted from 728 adults. Details of the study design and the data collected have been described on its website (www.epi.bris.ac.uk/boydorr) and elsewhere .
The Caerphilly Prospective Study (CaPS) recruited 2512 men aged between 45 and 59 years in 1979–83 from the town of Caerphilly, South Wales, and its surrounding villages. Blood samples were collected at baseline and at each of the four follow-ups (Phase II: 1984–88, Phase III: 1989–93, Phase IV: 1993–97 and Phase V: 2002–04.) Further details are available on the cohort's website (www.epi.bris.ac.uk/caerphilly/caerphillyprospectivestudy.htm).
The Lothian Birth Cohort 1921 Study (LBC1921) participants were all born in 1921 and completed an IQ assessment at age 11. In 1999–2001 (Phase I) 550 79 year olds, living in and around Edinburgh, attended a clinic. Details of the recruitment into the study are available on its website (www.lothianbirthcohort.ed.ac.uk) and have been published previously .
Genotyping and Quality Control
Genotyping for SNPs rs35767 (−C1245T, IGF1), rs7127900 (IGF2), rs2854744 (A-202C, IGFBP3), rs2943641 (IRS1) and rs2665802 (T1663A/T1169A, GH1) for all cohorts, except LBC1921, were carried out by KBioscience (www.kbioscience.co.uk). Genotype information for rs7127900 (IGF2) and rs2943641 (IRS1) in LBC1921 came from a genome-wide scan performed on the Illumina Human610-Quadv1 Chip (www.illumina.com) . The d3GHR (exon-3 deletion of GHR) polymorphism resulted from recombination between two near identical retroelements and can therefore be genotyped using a “pseudo-SNP” assay for a single nucleotide difference between the two alleles . Genotyping for the d3GHR pseudo-SNP was performed by KBioscience. Data quality was reviewed by assessing clustering quality (using KBioscience software SNPviewer on their data), call rates and deviation from Hardy-Weinberg equilibrium (HWE). Where deviation from HWE was detected for a study based on the hypothesis test, no exclusions were made due to the high quality of the studies in this investigation and the consistent genotyping methods used for the majority of the polymorphisms across the studies .
Measurements were conducted either at clinics, during a clinical interview in the home, or from self-report. Body mass index (BMI kg/m2) was calculated as weight divided by height squared. Waist-hip ratio (WHR) was defined as waist circumference (cm) divided by hip circumference (cm) and was measured in NSHD, ELSA, HCS, HAS, Boyd Orr and CaPS.
Physical Capability and Activity.
Grip strength was measured in NSHD, ELSA, HCS, HAS and LBC1921 using electronic or hydraulic dynamometers, with the best measure used in the analysis where more than one trial was conducted. Standing balance tests were conducted in the studies, with participants' eyes open: flamingo , (stopped at 30 s) in NSHD, HCS, HAS, Boyd Orr and CaPS, and side-by-side, semi-tandem and full tandem  in ELSA. Poor standing balance was defined for this analysis as the inability to complete 5 s. The timed get up and go test  was carried out in HCS, HAS, Boyd Orr and CaPS and required participants to get up from a chair, walk 3 m, turn, walk back, turn and sit down. Timed walks over 2.44 m (8 feet) and 6 m were carried out in ELSA and LBC1921 respectively. Speeds were calculated for timed walks and get up and go, with the fastest speeds used in the analysis where more than one trial was conducted. Timed chair rises  involved asking participants to rise from a chair and sit back down 5 times in ELSA, HCS and HAS, and 10 times in NSHD; the reciprocal of time taken in seconds ×100  was used in the analysis. Levels of physical activity were derived from self reported levels using questionnaires in NSHD, ELSA, Boyd Orr, CaPS and LBC1921. Individuals were categorised as ‘physically active’ in this analysis if they engaged, at least once a month, in at least moderate sport or activities in NSHD, Boyd Orr, CaPS and LBC1921 or vigorous sport or activities in ELSA.
Where information on ancestry was collected, non-European participants were excluded from the analyses in order to avoid confounding from population stratification . Within studies, linear and logistic regression analyses were conducted on the continuous and dichotomous traits within the cohorts respectively, adjusting for sex in all studies except CaPS, and age in all studies except NSHD and LBC1921. Analyses of physical capability measures were repeated additionally adjusting for height and weight. Due to the low frequency of individuals homozygous for the T allele of rs35767 (n = 296, 2.3%), T allele of rs7127900 (n = 455, 3.5%) and the exon-3 deletion of GHR (n = 853, 6.5%), dominant models were used for these polymorphisms in order to avoid the presentation of tables containing cells with very low frequencies in particular cohorts. Additive models were used for rs2854744, rs2943641 and rs2665802 with genotypes coded as 0, 1 and 2 for the number of minor alleles. Likelihood ratio tests were used to compare the fit of the additive models compared with the full genotype model. For continuous traits, the normality of the standardised residuals was inspected with distributional diagnostic plots. Cook's distances  were plotted against fitted values, using a cut-off of four divided by sample size, to identify influential outliers in the continuous phenotypes. For the harmonisation of continuous traits that were used to obtain pooled estimates of the genotypic effects, z-score units were calculated in each study by subtracting the study mean and dividing by its standard deviation. The overall mean for z-scores is 0 and standard deviation 1. Beta coefficients calculated on z-score units can be reverted to the original scale by multiplying by an appropriate standard deviation. Two-step  meta-analyses using a random-effects model were performed to obtain pooled genotypic effects. The I2 measure was used to quantify heterogeneity . Additionally, within-study analyses of the physical capability traits were stratified by physical activity, an indicator shown to modify genotypic effects on anthropometric measures  and the effects of rs2665802 on colorectal cancer risk . Finally, the calculation of z-scores, for the continuous traits, and the main analyses were repeated in males and females separately. Quanto  was used for power calculations. Reporting of the analyses met the appropriate items of recommended checklists , . A two-tailed significance level of p<0.05 was used as evidence of statistical significance. Statistical analysis was performed in Stata 11.1 (StataCorp LP).
Cohort Summaries and Genotyping Quality
Relevant genotypic and phenotypic data were available for a total of 13,364 adults aged between 52 and 90 years old (Table 1). The call rates were high, exceeding 95% across the studies for all polymorphisms. The HWE condition was met for rs35767, rs7127900, rs2854744, rs2943641 and rs2665802 in all studies (p-values>0.09) except for rs7127900 in HAS (p-value = 0.001) and rs2665802 in LBC1921 (p-value = 0.02). Whilst the HWE condition was met for d3GHR in ELSA, HAS, Boyd Orr and CaPS (p-values>0.09) it was not met in two of the larger studies, NSHD (p-value = 0.02) and HCS (p-value = 0.04), and was borderline in LBC1921 (p-value = 0.06), with the number of d3/d3 individuals under-represented.
Associations between Genotypes and Phenotypes
In ELSA, the study with the widest age range, genotype frequencies in those under 70 years were compared with those over 70 years; the T allele of rs2943641 (IRS1) was more common among those over 70 (chi-squared p-value = 0.0059). Associations with age group were not observed for the other polymorphisms (p-values>0.1; data not shown).
Figure 1 and Tables S1, S2, S3, S4, S5, S6 show the associations between the polymorphisms and height, weight, BMI and WHR adjusting for age and sex. From the pooled analyses there was evidence for associations between d3GHR and weight (p-value = 0.048) and BMI (p-value = 0.005), with individuals with at least one exon-3 deletion allele having lower weight and BMI compared with individuals retaining both copies of the exon (Figure S1 and S2). There was also a trend among individuals carrying the exon-3 deletion to have a lower WHR (p-value = 0.07) and for rs2854744-A (IGFBP3) to be associated with shorter height (p-value = 0.08). There was no evidence for other associations between the polymorphisms and anthropometric measures (p-values>0.1).
Adjusted for age and sex. Models used: rs35767 (IGF1)- (C/T+T/T) vs. C/C; rs7127900 (IGF2)- (C/T+T/T) vs. C/C; rs2854744 (IGFBP3- per minor (A) allele; rs2943641 (IRS1)- per minor (T) allele; rs2665802 (GH1)- per minor (T) allele; d3GHR- (fl/d3+d3/d3) vs. fl/fl. fl: full length; d3: exon-3 deletion.
Figure 2 and Tables S1, S2, S3, S4, S5, S6 show that there was no evidence for associations between any of the polymorphisms and grip strength, timed get up and go/walks and timed chair rises after adjusting for age and sex (p-values>0.1). Similarly, there were no associations after additionally adjusting for height and weight (p-values>0.1; data not shown).
Adjusted for age and sex. Timed chair rises on reciprocal of time taken in sec ×100. Models used: rs35767 (IGF1)- (C/T+T/T) vs. C/C; rs7127900 (IGF2)- (C/T+T/T) vs. C/C; rs2854744 (IGFBP3- per minor (A) allele; rs2943641 (IRS1)- per minor (T) allele; rs2665802 (GH1)- per minor (T) allele; d3GHR- (fl/d3+d3/d3) vs. fl/fl. fl: full length; d3: exon-3 deletion.
The associations between the polymorphisms and poor balance, adjusting for age and sex, are presented in Figure 3 and Tables S1, S2, S3, S4, S5, S6. There was evidence for an association with SNP rs2665802 (GH1) (p-value = 0.01), with the minor allele associated with increased ability to balance (Figure S3). The association remained after additional adjustment for height and weight (p-value = 0.02; data not shown). There was no evidence for associations with the other polymorphisms after adjusting for age, sex (p-values>0.5), height and weight (p-values>0.4; data not shown).
Adjusted for age and sex. Poor balance defined as inability to complete the Flamingo test for 5 s in Boyd Orr, HAS, HCS, NSHD and CaPS, and 5 s of the tandem test in ELSA. Models used: rs35767 (IGF1)- (C/T+T/T) vs. C/C; rs7127900 (IGF2)- (C/T+T/T) vs. C/C; rs2854744 (IGFBP3- per minor (A) allele; rs2943641 (IRS1)- per minor (T) allele; rs2665802 (GH1)- per minor (T) allele; d3GHR- (fl/d3+d3/d3) vs. fl/fl. fl: full length; d3: exon-3 deletion.
Within NSHD, ELSA, Boyd Orr, CaPS and LBC1921, there was little evidence that the genotypic effects for the physical capability traits differed between the physically active and inactive. Interactions were only observed in Boyd Orr for rs2854744 with balance (p-value = 0.04) and in ELSA for rs2943641 with grip strength (p-value = 0.03); however, the genotypic effects were not significant in either group in ELSA, whilst an association was observed with balance in the physically inactive group in Boyd Orr.
Investigations by Sex
Figures S4 and S5 present the pooled associations between the polymorphisms and the anthropometric traits adjusting for age in males and females, respectively. There was evidence for a sex difference between rs2943641 (IRS1) and height (p-value = 0.03 for heterogeneity between males and females) with the T allele being associated with greater height in females but not males (Figure S6). There was no evidence for any other sex differences between the polymorphisms and anthropometric traits (heterogeneity p-values>0.1).
Pooled associations between the polymorphisms and the physical capability traits, adjusting for age, in males and females are presented in Figures S7 to S9. There was no evidence for sex differences (heterogeneity p-values>0.1), except for rs35767 (IGF1) and chair rises (heterogeneity p-value = 0.03) where the genotypic effects were in opposite directions, though not achieving statistical significance in either sex (Figure S10).
We examined associations between six common polymorphisms in the GH/IGF axis and measures of anthropometric and physical capability phenotypes in seven UK cohorts comprising a total of 13,364 adults aged between 52 and 90 years. To our knowledge this is the largest investigation into polymorphisms in the axis and physical capability. The polymorphisms chosen are either functional or have been robustly associated with age-related phenotypes in GWAS. Among the 48 genotype association tests conducted in the main analysis there were few important associations observed. We found evidence for associations between d3GHR and anthropometry, with carriers of at least one exon-3 deletion having lower weight, BMI and a trend towards lower WHR. After adjusting for age and sex we found evidence for an association with physical capability for only one of the polymorphisms, with the minor allele of rs2665802 (GH1) being associated with increased ability to balance for at least 5 s. On the whole, the associations for physical capability were similar in males and females, between the physically active and inactive and after additionally adjusting for height and weight. These findings suggest that these polymorphisms are not important contributors to physical capability in older adults.
GWAS in Asian populations have found associations between SNPs around IGF1, including rs35767, and height , . However, to-date, little evidence has emerged from GWAS supporting highly significant associations between common polymorphisms in the six genes that we investigated in the GH/IGF axis and measures of anthropometry in Europeans , . Although, it is possible that SNPs in these six genes may significantly modify the effects on lean body mass of SNPs in other genes that are relevant to the GH/IGF axis . We observed no association between rs35767 (IGF1) and measures of body size, consistent with other studies on European populations , . A previous investigation in elderly men and women found no consistent associations between rs35767 (IGF1) and measures of body composition and walk times, grip strength and timed chair stand . We did not observe poorer measures of physical capability among carriers of the risk allele for prostate cancer for rs7127900 near IGF2 . Other smaller studies had found no association between rs2854744 (IGFBP3) and measures of adult body size in women of European ancestry (n = 1702) , or muscle phenotypes in response to strength training in older adults (n = 128) . Unlike a previous study (n = 454) , we did not observe associations between rs2665802 (GH1) and height in either sex, nor were associations reported with height or BMI elsewhere (sample sizes ranged between 293 and 1003) , , . Our results suggested that the minor allele of rs2665802 (GH1) was associated with better ability to balance for at least 5 s. The allele has also been associated with lower colorectal cancer  and mortality risk in females , though the null findings observed for the other measures of physical capability would suggest that rs2665802 (GH1) does not substantially influence measures of physical capability. In addition, a study of 169 athletes and 155 controls suggests that it is not associated with athletic status . Our meta-analysis provided some evidence for lower weight and BMI among carriers of the exon-3 deletion of GHR. Although few specific investigations into the d3GHR polymorphism on population-based studies of adults have been conducted, smaller studies have found no associations with measures of body size (sample sizes ranged between 100 and 831) , , ; however, one study observed associations between its haplotypic block and BMI . We therefore contacted the GIANT Consortium  to look up associations between tag SNPs of d3GHR and BMI. No associations were observed in per minor allele models for BMI for any of the five available SNPs, for example, for rs4590183 the data suggested a p-value of 0.98 in an analysis of 123,863 participants, suggesting our observed associations for d3GHR with weight and BMI were false positives.
It is an important finding that this investigation does not provide evidence for a substantial role of these common polymorphisms in the GH/IGF axis and measures of physical capability given the statistical power of this multi-cohort study. Sample size calculations for the quantitative traits for 80% power at the 5% significance level estimated that around 7000 individuals would be required to detect a beta coefficient of 0.07 z-score units under a dominant model for a polymorphism with a MAF of 0.19 or for 0.05 z-score units under an additive model using a MAF of 0.35. For example, there was sufficient power to detect a difference in grip strength of around 0.8 kg under a dominant model for rs7127900 (IGF2), assuming a standard deviation of 11. This allows the inference that any associations between the polymorphisms and physical capability traits are very small.
Deviation from the HWE condition was detected for d3GHR in two of the larger studies, NSHD and HCS. However, the only suggested associations for d3GHR were with weight and BMI, which were found to be in opposite directions for these studies (Table S6, Figure S1 and S2), indicating that, although exclusion is not recommended , excluding these two studies would not substantially affect the overall pooled results. Furthermore, whilst we observed four deviations from HWE altogether, around two statistically significant tests would be expected given the 42 (6 polymorphisms×7 studies) HWE tests performed.
Whilst the choice of polymorphisms within our candidate genes were primarily based on robust associations observed with age-related traits , , , , or functional evidence , additional polymorphisms in these genes may be useful for exploring further the influence of common polymorphisms in the GH/IGF axis on measures of physical capability.
The results of this large, multi-cohort investigation do not support the hypothesis that these common polymorphisms in the GH/IGF axis contribute substantially to objectively measured physical capability in older adults.
Meta-analysis for the Associations between d3GHR Genotype and Weight. Adjusted for age and sex. Coefficients based on z-scores. fl: full length; d3: exon-3 deletion.
Meta-analysis for the Associations between d3GHR Genotype and BMI. Adjusted for age and sex. Coefficients based on z-scores. fl: full length; d3: exon-3 deletion.
Meta-analysis for the Associations between rs2665802 (GH1) Genotype and Poor Balance. Adjusted for age, sex. Poor balance defined as inability to complete the Flamingo test for 5 s in Boyd Orr, HAS, HCS, NSHD and CaPS, and 5 s of the tandem test in ELSA.
Summary of Pooled Results of Associations between Genotypes and Measures of Anthropometry in Males. Adjusted for age. Models used: rs35767 (IGF1)- (C/T+T/T) vs. C/C; rs7127900 (IGF2)- (C/T+T/T) vs. C/C; rs2854744 (IGFBP3- per minor (A) allele; rs2943641 (IRS1)- per minor (T) allele; rs2665802 (GH1)- per minor (T) allele; d3GHR- (fl/d3+d3/d3) vs. fl/fl. fl: full length; d3: exon-3 deletion.
Summary of Pooled Results of Associations between Genotypes and Measures of Anthropometry in Females. Adjusted for age. Models used: rs35767 (IGF1)- (C/T+T/T) vs. C/C; rs7127900 (IGF2)- (C/T+T/T) vs. C/C; rs2854744 (IGFBP3- per minor (A) allele; rs2943641 (IRS1)- per minor (T) allele; rs2665802 (GH1)- per minor (T) allele; d3GHR- (fl/d3+d3/d3) vs. fl/fl. fl: full length; d3: exon-3 deletion.
Meta-analysis for the Associations between rs2943641 (IRS1) Genotype and Height by Sex. Adjusted for age. Coefficients based on z-scores.
Summary of Pooled Results of Associations between Genotypes and Grip Strength, Timed Get Up & Go and Chair Rises in Males. Adjusted for age. Timed chair rises on reciprocal of time taken in sec ×100. Models used: rs35767 (IGF1)- (C/T+T/T) vs. C/C; rs7127900 (IGF2)- (C/T+T/T) vs. C/C; rs2854744 (IGFBP3- per minor (A) allele; rs2943641 (IRS1)- per minor (T) allele; rs2665802 (GH1)- per minor (T) allele; d3GHR- (fl/d3+d3/d3) vs. fl/fl. fl: full length; d3: exon-3 deletion.
Summary of Pooled Results of Associations between Genotypes and Grip Strength, Timed Get Up & Go and Chair Rises in Females. Adjusted for age. Timed chair rises on reciprocal of time taken in sec ×100. Models used: rs35767 (IGF1)- (C/T+T/T) vs. C/C; rs7127900 (IGF2)- (C/T+T/T) vs. C/C; rs2854744 (IGFBP3- per minor (A) allele; rs2943641 (IRS1)- per minor (T) allele; rs2665802 (GH1)- per minor (T) allele; d3GHR- (fl/d3+d3/d3) vs. fl/fl. fl: full length; d3: exon-3 deletion.
Summary of Pooled Results of Associations between Genotypes and Poor Balance by Sex. Adjusted for age. Poor balance defined as inability to complete the Flamingo test for 5 s in Boyd Orr, HAS, HCS, NSHD and CaPS, and 5 s of the tandem test in ELSA. Models used: rs35767 (IGF1)- (C/T+T/T) vs. C/C; rs7127900 (IGF2)- (C/T+T/T) vs. C/C; rs2854744 (IGFBP3- per minor (A) allele; rs2943641 (IRS1)- per minor (T) allele; rs2665802 (GH1)- per minor (T) allele; d3GHR- (fl/d3+d3/d3) vs. fl/fl. fl: full length; d3: exon-3 deletion.
Meta-analysis for the Associations between rs35767 (IGF1) Genotype and Timed Chair Rises by Sex. Adjusted for age. Timed chair rises on reciprocal of time taken in sec ×100. Coefficients based on z-scores.
Anthropometry and Physical Capability by rs35767 ( IGF1 ) Genotype and Cohort.
Anthropometry and Physical Capability by rs7127900 ( IGF2 ) Genotype and Cohort.
Anthropometry and Physical Capability by rs2854744 ( IGFBP3 ) Genotype and Cohort.
Anthropometry and Physical Capability by rs2943641 ( IRS1 ) Genotype and Cohort.
Anthropometry and Physical Capability by rs2665802 ( GH1 ) Genotype and Cohort.
Anthropometry and Physical Capability by d3GHR Genotype and Cohort.
We thank the GIANT Consortium, in particular Ruth Loos, for the SNP look ups.
We thank Kate Birnie, Vanessa Cox, Nikki Graham, Karen Jameson, Andrew Taylor and Andrew Wong for providing data.
The Caerphilly Prospective study was conducted by the former MRC Epidemiology Unit (South Wales). The School of Social and Community Medicine, University of Bristol now maintains the archive.
ELSA was developed by a team of researchers based at the National Centre for Social Research, University College London and the Institute of Fiscal Studies. The data were collected by the National Centre for Social Research.
The Hertfordshire Cohort Study and the Hertfordshire Ageing Study were conducted by the MRC Lifecourse Epidemiology Unit at the University of Southampton.
We thank the Lothian Birth Cohort 1921 participants. We thank the Scottish Council for Research in Education for allowing access to the Scottish Mental Survey 1932.
The HALCyon study team also includes Jane Elliott, Catharine Gale, James Goodwin, Alison Lennox, Marcus Richards, Thomas von Zglinicki, John Gallacher, Gita Mishra, Chris Power, Paul Shiels, Humphrey Southall, Andrew Steptoe, Panos Demakakos, Kate Tilling, Lawrence Whalley, Geraldine McNeill, Leone Craig, Carmen Martin-Ruiz, Paula Aucott, Emily Murray, Zeinab Mulla, Mike Gardner and Sam Parsons.
Conceived and designed the experiments: I.Day. Analyzed the data: TA. Wrote the paper: TA. Advised on the statistical analyses and contributed to the manuscript: I.Day YB-S. Contributed the HALCyon programme: DK. Contributed studies to the project: YB-S CC I.Deary MK RM AS DK. Provided comments on the manuscript: RC RH CC I.Deary TG DG SH MK RM AS JS DK. Designed the GHR assay: TG.
- 1. Baumgartner RN, Koehler KM, Gallagher D, Romero L, Heymsfield SB, et al. (1998) Epidemiology of sarcopenia among the elderly in New Mexico. Am J Epidemiol 147: 755–763.
- 2. Janssen I (2006) Influence of sarcopenia on the development of physical disability: the Cardiovascular Health Study. J Am Geriatr Soc 54: 56–62. doi:10.1111/j.1532-5415.2005.00540.x.
- 3. Abellan van Kan G (2009) Epidemiology and consequences of sarcopenia. J Nutr Health Aging 13: 708–712.
- 4. Lauretani F, Russo CR, Bandinelli S, Bartali B, Cavazzini C, et al. (2003) Age-associated changes in skeletal muscles and their effect on mobility: an operational diagnosis of sarcopenia. J Appl Physiol 95: 1851–1860. doi:10.1152/japplphysiol.00246.2003.
- 5. Szulc P, Munoz F, Marchand F, Chapurlat R, Delmas PD (2010) Rapid loss of appendicular skeletal muscle mass is associated with higher all-cause mortality in older men: the prospective MINOS study. Am J Clin Nutr 91: 1227–1236. doi:10.3945/ajcn.2009.28256.
- 6. Abbatecola AM, Ferrucci L, Ceda G, Russo CR, Lauretani F, et al. (2005) Insulin resistance and muscle strength in older persons. J Gerontol A Biol Sci Med Sci 60: 1278–1282.
- 7. Szulc P, Beck TJ, Marchand F, Delmas PD (2005) Low skeletal muscle mass is associated with poor structural parameters of bone and impaired balance in elderly men–the MINOS study. J Bone Miner Res 20: 721–729. doi:10.1359/JBMR.041230.
- 8. Cooper R, Kuh D, Hardy R, Mortality Review Group, FALCon and HALCyon study teams. (2010) Objectively measured physical capability levels and mortality: systematic review and meta-analysis. BMJ 341: c4467–c4467. doi:10.1136/bmj.c4467.
- 9. Guralnik JM, Ferrucci L, Simonsick EM, Salive ME, Wallace RB (1995) Lower-extremity function in persons over the age of 70 years as a predictor of subsequent disability. N Engl J Med 332: 556–561. doi:10.1056/NEJM199503023320902.
- 10. Cooper R, Kuh D, Cooper C, Gale CR, Lawlor DA, et al. (2011) Objective measures of physical capability and subsequent health: a systematic review. Age Ageing 40: 14–23. doi:10.1093/ageing/afq117.
- 11. Giovannini S, Marzetti E, Borst SE, Leeuwenburgh C (2008) Modulation of GH/IGF-1 axis: potential strategies to counteract sarcopenia in older adults. Mech Ageing Dev 129: 593–601. doi:10.1016/j.mad.2008.08.001.
- 12. Velloso CP (2008) Regulation of muscle mass by growth hormone and IGF-I. Br J Pharmacol 154: 557–568. doi:10.1038/bjp.2008.153.
- 13. Chargé SBP, Rudnicki MA (2004) Cellular and molecular regulation of muscle regeneration. Physiol Rev 84: 209–238. doi:10.1152/physrev.00019.2003.
- 14. Clemmons DR (2009) Role of IGF-I in skeletal muscle mass maintenance. Trends Endocrinol. Metab 20: 349–356. doi:10.1016/j.tem.2009.04.002.
- 15. Lang T, Streeper T, Cawthon P, Baldwin K, Taaffe DR, et al. (2010) Sarcopenia: etiology, clinical consequences, intervention, and assessment. Osteoporos Int 21: 543–559. doi:10.1007/s00198-009-1059-y.
- 16. Baserga R (2009) The insulin receptor substrate-1: a biomarker for cancer? Exp Cell Res 315: 727–732. doi:10.1016/j.yexcr.2008.09.017.
- 17. Sotiropoulos A, Ohanna M, Kedzia C, Menon RK, Kopchick JJ, et al. (2006) Growth hormone promotes skeletal muscle cell fusion independent of insulin-like growth factor 1 up-regulation. Proc Natl Acad Sci U S A 103: 7315–7320. doi:10.1073/pnas.0510033103.
- 18. Chen W, Wang S, Tian T, Bai J, Hu Z, et al. (2009) Phenotypes and genotypes of insulin-like growth factor 1, IGF-binding protein-3 and cancer risk: evidence from 96 studies. Eur J Hum Genet 17: 1668–1675. doi:10.1038/ejhg.2009.86.
- 19. Bartke A (2005) Minireview: role of the growth hormone/insulin-like growth factor system in mammalian aging. Endocrinology 146: 3718–3723. doi:10.1210/en.2005-0411.
- 20. Berryman DE, Christiansen JS, Johannsson G, Thorner MO, Kopchick JJ (2008) Role of the GH/IGF-1 axis in lifespan and healthspan: lessons from animal models. Growth Horm IGF Res 18: 455–471. doi:10.1016/j.ghir.2008.05.005.
- 21. Rudman D, Feller AG, Nagraj HS, Gergans GA, Lalitha PY, et al. (1990) Effects of human growth hormone in men over 60 years old. N Engl J Med 323: 1–6.
- 22. Tiainen K, Sipila S, Alen M, Heikkinen E, Kaprio J, et al. (2004) Heritability of maximal isometric muscle strength in older female twins. J Appl Physiol 96: 173–180. doi:10.1152/japplphysiol.00200.2003.
- 23. Matteini AM, Fallin MD, Kammerer CM, Schupf N, Yashin AI, et al. (2010) Heritability estimates of endophenotypes of long and health life: the Long Life Family Study. J Gerontol A Biol Sci Med Sci 65: 1375–1379. doi:10.1093/gerona/glq154.
- 24. Yang T-L, Xiong D-H, Guo Y, Recker RR, Deng H-W (2008) Comprehensive association analyses of IGF1, ESR2, and CYP17 genes with adult height in Caucasians. Eur J Hum Genet 16: 1380–1387. doi:10.1038/ejhg.2008.113.
- 25. Kim J-J, Lee H-I, Park T, Kim K, Lee J-E, et al. (2010) Identification of 15 loci influencing height in a Korean population. J Hum Genet 55: 27–31. doi:10.1038/jhg.2009.116.
- 26. Okada Y, Kamatani Y, Takahashi A, Matsuda K, Hosono N, et al. (2010) A genome-wide association study in 19 633 Japanese subjects identified LHX3-QSOX2 and IGF1 as adult height loci. Hum Mol Genet 19: 2303–2312. doi:10.1093/hmg/ddq091.
- 27. Dupuis J, Langenberg C, Prokopenko I, Saxena R, Soranzo N, et al. (2010) New genetic loci implicated in fasting glucose homeostasis and their impact on type 2 diabetes risk. Nat Genet 42: 105–116. doi:10.1038/ng.520.
- 28. Schumacher FR, Cheng I, Freedman ML, Mucci L, Allen NE, et al. (2010) A comprehensive analysis of common IGF1, IGFBP1 and IGFBP3 genetic variation with prospective IGF-I and IGFBP-3 blood levels and prostate cancer risk among Caucasians. Hum Mol Genet 19: 3089–3101. doi:10.1093/hmg/ddq210.
- 29. Palles C, Johnson N, Coupland B, Taylor C, Carvajal J, et al. (2008) Identification of genetic variants that influence circulating IGF1 levels: a targeted search strategy. Hum Mol Genet 17: 1457–1464. doi:10.1093/hmg/ddn034.
- 30. Patel AV, Cheng I, Canzian F, Le Marchand L, Thun MJ, et al. (2008) IGF-1, IGFBP-1, and IGFBP-3 polymorphisms predict circulating IGF levels but not breast cancer risk: findings from the Breast and Prostate Cancer Cohort Consortium (BPC3). PLoS ONE 3: e2578. doi:10.1371/journal.pone.0002578.
- 31. Stessman J, Maaravi Y, Hammerman-Rozenberg R, Cohen A, Nemanov L, et al. (2005) Candidate genes associated with ageing and life expectancy in the Jerusalem longitudinal study. Mech Ageing Dev 126: 333–339. doi:10.1016/j.mad.2004.08.025.
- 32. Heude B, Ong KK, Luben R, Wareham NJ, Sandhu MS (2007) Study of association between common variation in the insulin-like growth factor 2 gene and indices of obesity and body size in middle-aged men and women. J Clin Endocrinol Metab 92: 2734–2738. doi:10.1210/jc.2006-1948.
- 33. Zhang W, Maniatis N, Rodriguez S, Miller GJ, Day INM, et al. (2006) Refined association mapping for a quantitative trait: weight in the H19-IGF2-INS-TH region. Ann Hum Genet 70: 848–856. doi:10.1111/j.1469-1809.2006.00290.x.
- 34. Gaunt TR, Cooper JA, Miller GJ, Day IN, O'Dell SD (2001) Positive associations between single nucleotide polymorphisms in the IGF2 gene region and body mass index in adult males. Hum Mol Genet 10: 1491–1501.
- 35. Bachner-Melman R, Zohar AH, Nemanov L, Heresco-Levy U, Gritsenko I, et al. (2005) Association between the insulin-like growth factor 2 gene (IGF2) and scores on the Eating Attitudes Test in nonclinical subjects: a family-based study. Am J Psychiatry 162: 2256–2262. doi:10.1176/appi.ajp.162.12.2256.
- 36. Sayer AA, Syddall H, O'Dell SD, Chen X-H, Briggs PJ, et al. (2002) Polymorphism of the IGF2 gene, birth weight and grip strength in adult men. Age Ageing 31: 468–470.
- 37. Schrager MA, Roth SM, Ferrell RE, Metter EJ, Russek-Cohen E, et al. (2004) Insulin-like growth factor-2 genotype, fat-free mass, and muscle performance across the adult life span. J Appl Physiol 97: 2176–2183. doi:10.1152/japplphysiol.00985.2003.
- 38. Devaney JM, Hoffman EP, Gordish-Dressman H, Kearns A, Zambraski E, et al. (2007) IGF-II gene region polymorphisms related to exertional muscle damage. J Appl Physiol 102: 1815–1823. doi:10.1152/japplphysiol.01165.2006.
- 39. Eeles RA, Kote-Jarai Z, Al Olama AA, Giles GG, Guy M, et al. (2009) Identification of seven new prostate cancer susceptibility loci through a genome-wide association study. Nat Genet 41: 1116–1121. doi:10.1038/ng.450.
- 40. Kaplan RC, Petersen A-K, Chen M-H, Teumer A, Glazer NL, et al. (2011) A genome-wide association study identifies novel loci associated with circulating IGF-I and IGFBP-3. Hum Mol Genet 20: 1241–1251. doi:10.1093/hmg/ddq560.
- 41. Rung J, Cauchi S, Albrechtsen A, Shen L, Rocheleau G, et al. (2009) Genetic variant near IRS1 is associated with type 2 diabetes, insulin resistance and hyperinsulinemia. Nat Genet 41: 1110–1115. doi:10.1038/ng.443.
- 42. Millar DS, Horan M, Chuzhanova NA, Cooper DN (2010) Characterisation of a functional intronic polymorphism in the human growth hormone (GH1) gene. Hum Genomics 4: 289–301.
- 43. Le Marchand L, Donlon T, Seifried A, Kaaks R, Rinaldi S, et al. (2002) Association of a common polymorphism in the human GH1 gene with colorectal neoplasia. J Natl Cancer Inst 94: 454–460.
- 44. van Heemst D, Beekman M, Mooijaart SP, Heijmans BT, Brandt BW, et al. (2005) Reduced insulin/IGF-1 signalling and human longevity. Aging Cell 4: 79–85. doi:10.1111/j.1474-9728.2005.00148.x.
- 45. Bougnères P (2010) The exon-3 deletion of the growth hormone receptor (GHR) gene still has a limited impact in clinical endocrinology. J Clin Endocrinol Metab 95: 56–59. doi:10.1210/jc.2009-2233.
- 46. McKay JD, Kaaks R, Johansson M, Biessy C, Wiklund F, et al. (2007) Haplotype-based analysis of common variation in the growth hormone receptor gene and prostate cancer risk. Cancer Epidemiol. Biomarkers Prev 16: 169–173. doi:10.1158/1055-9965.EPI-06-0320.
- 47. Van Dyke AL, Cote ML, Wenzlaff AS, Abrams J, Land S, et al. (2009) Chromosome 5p Region SNPs Are Associated with Risk of NSCLC among Women. J Cancer Epidemiol 2009: 242151. doi:10.1155/2009/242151.
- 48. Wadsworth M, Kuh D, Richards M, Hardy R (2006) Cohort Profile: The 1946 National Birth Cohort (MRC National Survey of Health and Development). Int J Epidemiol 35: 49–54. doi:10.1093/ije/dyi201.
- 49. Marmot M, Banks J, Blundell R, Lessof C, Nazroo J (2003) Health, wealth and lifestyles of the older population in England: The 2002 English Longitudinal Study of Ageing. London: Institute for Fiscal Studies.
- 50. Syddall H (2005) Cohort Profile: The Hertfordshire Cohort Study. Int J Epidemiol 34: 1234–1242. doi:10.1093/ije/dyi127.
- 51. Syddall HE, Simmonds SJ, Martin HJ, Watson C, Dennison EM, et al. (2009) Cohort profile: The Hertfordshire Ageing Study (HAS). Int J Epidemiol 39: 36–43. doi:10.1093/ije/dyn275.
- 52. Martin RM, Gunnell D, Pemberton J, Frankel S, Davey Smith G (2005) Cohort profile: The Boyd Orr cohort–an historical cohort study based on the 65 year follow-up of the Carnegie Survey of Diet and Health (1937–39). Int J Epidemiol 34: 742–749. doi:10.1093/ije/dyi124.
- 53. Deary IJ, Whiteman MC, Starr JM, Whalley LJ, Fox HC (2004) The impact of childhood intelligence on later life: following up the Scottish mental surveys of 1932 and 1947. J Pers Soc Psychol 86: 130–147. doi:10.1037/0022-3522.214.171.124.
- 54. Houlihan LM, Davies G, Tenesa A, Harris SE, Luciano M, et al. (2010) Common Variants of Large Effect in F12, KNG1, and HRG Are Associated with Activated Partial Thromboplastin Time. Am J Hum Genet 86: 626–631. doi:10.1016/j.ajhg.2010.02.016.
- 55. Gaunt TR, Hinks LJ, Christensen MB, Kiessling M, Day IN (2005) Experience applying lightTyper methodology to human SNP's relevant to growth and cardiovascular risk. In: Hecker K, editor. Genetic variance detection: technologies for pharmacogenomics. DNA Press. In:. pp. 131–144.
- 56. Minelli C, Thompson JR, Abrams KR, Thakkinstian A, Attia J (2008) How should we use information about HWE in the meta-analyses of genetic association studies? Int J Epidemiol 37: 136–146. doi:10.1093/ije/dym234.
- 57. Committee of Experts on Sports Research (1993) Eurofit: handbook for the EUROFIT tests of physical fitness. Strasbourg: Council of Europe. 2nd Edition.
- 58. Stevens KN, Lang IA, Guralnik JM, Melzer D (2008) Epidemiology of balance and dizziness in a national population: findings from the English Longitudinal Study of Ageing. Age Ageing 37: 300–305. doi:10.1093/ageing/afn019.
- 59. Podsiadlo D, Richardson S (1991) The timed “Up & Go”: a test of basic functional mobility for frail elderly persons. J Am Geriatr Soc 39: 142–148.
- 60. Csuka M, McCarty DJ (1985) Simple method for measurement of lower extremity muscle strength. Am J Med 78: 77–81.
- 61. Kuh D, Bassey EJ, Butterworth S, Hardy R, Wadsworth MEJ (2005) Grip strength, postural control, and functional leg power in a representative cohort of British men and women: associations with physical activity, health status, and socioeconomic conditions. J Gerontol A Biol Sci Med Sci 60: 224–231.
- 62. Cordell HJ, Clayton DG (2005) Genetic association studies. Lancet 366: 1121–1131. doi:10.1016/S0140-6736(05)67424-7.
- 63. Cook RD (2000) Detection of Influential Observation in Linear Regression. Technometrics 42: 65–68.
- 64. Riley RD, Lambert PC, Abo-Zaid G (2010) Meta-analysis of individual participant data: rationale, conduct, and reporting. BMJ 340: c221. doi:10.1136/bmj.c221.
- 65. Higgins JPT, Thompson SG, Deeks JJ, Altman DG (2003) Measuring inconsistency in meta-analyses. BMJ 327: 557–560. doi:10.1136/bmj.327.7414.557.
- 66. Li S, Zhao JH, Luan J, Ekelund U, Luben RN, et al. (2010) Physical Activity Attenuates the Genetic Predisposition to Obesity in 20,000 Men and Women from EPIC-Norfolk Prospective Population Study. PLoS Med 7: e1000332. doi:10.1371/journal.pmed.1000332.
- 67. Khoury-Shakour S, Gruber SB, Lejbkowicz F, Rennert HS, Raskin L, et al. (2008) Recreational physical activity modifies the association between a common GH1 polymorphism and colorectal cancer risk. Cancer Epidemiol. Biomarkers Prev 17: 3314–3318. doi:10.1158/1055-9965.EPI-08-0062.
- 68. Gauderman W, Morrison J (2006) QUANTO 1.1: A computer program for power and sample size calculations for genetic-epidemiology studies, [http://hydra.usc.edu/gxe/].
- 69. Stroup DF, Berlin JA, Morton SC, Olkin I, Williamson GD, et al. (2000) Meta-analysis of Observational Studies in Epidemiology: A Proposal for Reporting. JAMA 283: 2008–2012. doi:10.1001/jama.283.15.2008.
- 70. Little J, Higgins JPT, Ioannidis JPA, Moher D, Gagnon F, et al. (2009) Strengthening the reporting of genetic association studies (STREGA): an extension of the strengthening the reporting of observational studies in epidemiology (STROBE) statement. J Clin Epidemiol 62: 597–608.e4. doi:10.1016/j.jclinepi.2008.12.004.
- 71. Lango Allen H, Estrada K, Lettre G, Berndt SI, Weedon MN, et al. (2010) Hundreds of variants clustered in genomic loci and biological pathways affect human height. Nature 467: 832–838. doi:10.1038/nature09410.
- 72. Scuteri A, Sanna S, Chen W-M, Uda M, Albai G, et al. (2007) Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traits. PLoS Genet 3: e115. doi:10.1371/journal.pgen.0030115.
- 73. Liu X-G, Tan L-J, Lei S-F, Liu Y-J, Shen H, et al. (2009) Genome-wide association and replication studies identified TRHR as an important gene for lean body mass. Am J Hum Genet 84: 418–423. doi:10.1016/j.ajhg.2009.02.004.
- 74. Lettre G, Butler JL, Ardlie KG, Hirschhorn JN (2007) Common genetic variation in eight genes of the GH/IGF1 axis does not contribute to adult height variation. Hum Genet 122: 129–139. doi:10.1007/s00439-007-0385-4.
- 75. Kostek MC, Devaney JM, Gordish-Dressman H, Harris TB, Thompson PD, et al. (2010) A polymorphism near IGF1 is associated with body composition and muscle function in women from the Health, Aging, and Body Composition Study. Eur J Appl Physiol 110: 315–324. doi:10.1007/s00421-010-1500-0.
- 76. Sweeney C, Murtaugh MA, Baumgartner KB, Byers T, Giuliano AR, et al. (2005) Insulin-like growth factor pathway polymorphisms associated with body size in Hispanic and non-Hispanic white women. Cancer Epidemiol Biomarkers Prev 14: 1802–1809. doi:10.1158/1055-9965.EPI-05-0149.
- 77. Hand BD, Kostek MC, Ferrell RE, Delmonico MJ, Douglass LW, et al. (2007) Influence of promoter region variants of insulin-like growth factor pathway genes on the strength-training response of muscle phenotypes in older adults. J Appl Physiol 103: 1678–1687. doi:10.1152/japplphysiol.00420.2007.
- 78. Ren Z, Cai Q, Shu X-O, Cai H, Cheng J-R, et al. (2004) Genetic polymorphisms in the human growth hormone-1 gene (GH1) and the risk of breast carcinoma. Cancer 101: 251–257. doi:10.1002/cncr.20375.
- 79. Hietaniemi M, Pöykkö SM, Ukkola O, Päivänsalo M, Antero Kesäniemi Y (2005) IGF-I concentrations are positively associated with carotid artery atherosclerosis in women. Ann Med 37: 373–382. doi:10.1080/07853890510011967.
- 80. Walpole B, Noakes TD, Collins M (2006) Growth hormone 1 (GH1) gene and performance and post-race rectal temperature during the South African Ironman triathlon. Br J Sports Med 40: 145–150; discussion 145–150. doi:10.1136/bjsm.2005.020669.
- 81. Kenth G, Shao Z, Cole DEC, Goodyer CG (2007) Relationship of the human growth hormone receptor exon 3 genotype with final adult height and bone mineral density. J Clin Endocrinol Metab 92: 725–728. doi:10.1210/jc.2006-1695.
- 82. Adetunji OR, MacFarlane IA, Javadpour M, Alfirevic A, Pirmohamed M, et al. (2009) The d3/fl-GH receptor gene polymorphism does not influence quality of life and body composition in GH-deficient adults receiving GH replacement therapy. Eur J Endocrinol 161: 541–546. doi:10.1530/EJE-09-0405.
- 83. Willer CJ, Speliotes EK, Loos RJF, Li S, Lindgren CM, et al. (2009) Six new loci associated with body mass index highlight a neuronal influence on body weight regulation. Nat Genet 41: 25–34. doi:10.1038/ng.287.