Postcopulatory sexual selection is an important force in the evolution of reproductive traits, including sperm morphology. In birds, sperm morphology is known to be highly heritable and largely condition-independent. Theory predicts, and recent comparative work corroborates, that strong selection in such traits reduces intraspecific phenotypic variation. Here we show that some variation can be maintained despite extreme promiscuity, as a result of opposing, copulation-role-specific selection forces. After controlling for known correlates of siring success in the superb fairy-wren (Malurus cyaneus), we found that (a) lifetime extra-pair paternity success was associated with sperm with a shorter flagellum and relatively large head, and (b) males whose sperm had a longer flagellum and a relatively smaller head achieved higher within-pair paternity. In this species extrapair copulations occur in the same morning, but preceding, pair copulations during a female's fertile period, suggesting that shorter and relatively larger-headed sperm are most successful in securing storage (defense), whereas the opposite phenotype might be better at outcompeting stored sperm (offense). Furthermore, since cuckolding ability is a major contributor to differential male reproductive output, stronger selection on defense sperm competition traits might explain the short sperm of malurids relative to other promiscuous passerines.
Citation: Calhim S, Double MC, Margraf N, Birkhead TR, Cockburn A (2011) Maintenance of Sperm Variation in a Highly Promiscuous Wild Bird. PLoS ONE6(12): e28809. https://doi.org/10.1371/journal.pone.0028809
Editor: Brock Fenton, University of Western Ontario, Canada
Received: August 1, 2011; Accepted: November 15, 2011; Published: December 15, 2011
Copyright: © 2011 Calhim et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: Dr. Calhim was supported by a doctoral grant from the Portuguese Science and Technology Foundation (SFRH/BD/12040/2003; www.fct.pt), the Australian Research Council supported Dr. Cockburn (www.arc.gov.au), and Dr. Margraf was financially supported by the Swiss National Science Foundation (PA00A-117491; www.snf.ch). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Female promiscuity is a feature of the breeding system of most passerines, often leading to extrapair paternity (e.g. ). Therefore, fitness in males that breed in social pairs but engage in extrapair copulations is the combined success of both extrapair and within-pair siring (e.g. ). Sperm competition theory proposes that males are selected to both protect from and overcome a rival male's paternity assurance mechanisms, and that traits involved in paternity defense and offense might be under antagonistic forces –. Several studies have looked at inter-male variation in a given trait and its concurrent and separate effect in within-pair and extrapair reproductive success in birds, yet these studies are largely limited to secondary sexual traits (i.e. plumage, song) or age (reviewed in Table S1, n = 20 species). In many cases, the trait has a directional effect on extrapair paternity but no effect on own-nest paternity, with two notable exceptions: crown ultraviolet hue in blue tits (Cyanistes caeruleus, ) and tail length in cape sugarbirds (Promerops cafer, ) have opposite effects. Focus on primary sexual traits and male copulation roles has largely been restricted to empirical studies of controlled matings in invertebrates (e.g. –), social status manipulation in domestic fowl (e.g. ), hormonal manipulation in a wild passerine  and alternative mating tactics in centrarchid fish (e.g. , ).
Most long-term studies of birds have difficulty measuring lifetime measures of fitness, due to low assignment of extra-pair sires and/or tracking fitness of dispersers (e.g. , ). Yet, accurate measurement of selection in the wild is best achieved when not restricted to a spatio-temporal snapshot of an otherwise well studied system , . Lifetime paternity success provides a direct assessment of differential pre- and postcopulatory success . However, using data from natural, unobserved matings has inherent unknown confounding effects, such as inter-male variation in sperm competition risk (likelihood of being cuckolded), relative timing and number of copulations for a given clutch . The detailed knowledge of the unusual breeding biology of superb fairy-wrens (Malurus cyaneus) make this a good species to investigate the evolutionary consequences of sperm morphology variation on extrapair and within-pair siring success in a wild bird. Superb fairy-wrens are territorial, facultative cooperative breeders, with a permanent social pair bond , . Males are philopatric and both sexes acquire their social mates passively rather than through selection on phenotype . Males queue for dominance on their natal or adjacent territories , while females compete for rare vacancies through dispersal . By contrast, females show strong precopulatory mate choice of extragroup sires, preferring males that acquire the nuptial plumage early, an honest signal of male quality –. Regardless of the quality of their own social partner (e.g. when it molted), all females make pre-dawn extragroup forays to mate with these preferred males, usually two or three days before egg-laying , leading to extragroup paternity in almost all broods . Helpers (and/or neighbors) of attractive males gain some of these extragroup fertilizations , suggesting a hidden lek effect of dawn chorus displays  and/or ‘error-prone’ female choice . Remarkably, unless socially paired to a son, females always copulate with their social partner within thirty minutes of returning from the extragroup foray, and/ or with unrelated helpers (Cockburn & Double, unpublished data). Thus sperm competition is pervasive, a view supported by the relatively large size of the testes, proportion of sperm producing tissue and cloacal protuberance –.
Passerine sperm is morphologically complex, with helical shaped heads, a large, single fused mitochondrion wrapped around the flagellum (, , but see  for an exception), and move by rotation along the main axis . Both passerine sperm form and function exhibit considerable additive genetic variance and generally show low condition-dependence (–, but see  for an exception). Comparative work in birds has shown that, as sperm competition increases, mean sperm length increases, although not linearly , , and both inter-male and intra-male variation in sperm size is reduced –. Recent studies suggest that while there are significant associations between sperm morphology (absolute flagellum length and relative to head length) and sperm motility across species (, but see ), these can become uncoupled at the intraspecific level in taxa, especially in species under high sperm competition (, – but see ). It is therefore reasonable to assume that in taxa under strong sperm competition, one might find certain morphometric traits to be associated with cuckolding success and/or defense. We studied cuckolding success and defense in the superb fairy-wren.
This study was conducted on an intensively studied color-banded population at the Australian National Botanical Gardens, Canberra , , in which the breeding history, age and parentage are known for most birds , , . Ethical approval for this research was given by the Australian National University Animal Experimentation Ethics Committee (permits: F.BTZ.63.04 and F.BTZ.06.07).
Cuckolding success was measured as the lifetime number of illegitimate offspring that survived to four weeks after fledgling (when census provides the most reliable fitness measure; A. Cockburn, pers. comm.). Cuckolding success was computed as a categorical variable using the total number of extrapair offspring a male sired (see Fig. S1). Since the frequency distribution can be interpreted as bimodal, with modes at zero and 3–4 young (which correspond functionally to siring no young or a single brood of extrapair chicks), and a lowest point at 2 young (Fig. S1). Failing to acquire an extrapair copulation (EPC) or to convert an EPC into an extrapair fertilization, which are impossible to differentiate in our data, reflect low competitiveness. Also, since EPC forays are so time-restricted , one can assume that there is little between individual variation in the number or EPCs per bout. Therefore, males producing a single extrapair young can be considered to have low success as a sperm competitor. We therefore managed our data distribution by analyzing cuckolding success as a binomial response, where the two classes were 0–1 and 2+ young. Using this cut-off rather than 0–2 versus 3+ young is further supported, as (i) one of the two males producing two EPO gained 100% success in the brood in which it obtained extrapair paternity and (ii) the other was a subordinate individual that successfully cuckolded within its social group. Therefore, both males can be considered successful sperm competitors. Because male age is a strong predictor of extrapair fertilization success, through its positive effect on the nuptial plumage molt date , , male breeding experience (i.e. number of breeding seasons it was alive) was included as a covariate in the Generalized Linear Model (glm function with logit link and (quasi)binomial error distribution; n = 59 males). In order to assess the robustness of this analysis, it was repeated using GLM with negative binomial errors (glm.nb function), an alternative interpretation of Fig. S1 distribution (see Supplementary Material for more details). We present the results of the latter in the Supplementary Material). Cuckolding defense success refers to the proportion of sired fledglings in all broods sampled while a given male was dominant. Broods assigned to males while in an incestuous pair with their own mothers were excluded (n = 4 broods), since inbreeding avoidance by females may bias within-nest paternity success . We used Generalized Linear Mixed Models, GLMMs (lmer function with logit link and binomial error distribution), to estimate the fixed effects of sperm morphometric traits, with male identity incorporated as a random factor. Helper number was included as a covariate, since dominant males without helpers are cuckolded less, possibly since paternity assurance increases care , , . One measure of sperm defense per male (average number of sired young across broods) was not appropriate since the total number of fledglings, female identity and helper number differed across broods. A total of n = 255 broods belonging to 47 males was used.
Sperm samples were collected non-invasively by collecting the liquid part of the faeces (see ) from n = 59 adult males in December 2005. This method  has been shown to provide reliable sperm morphometry data. Sperm morphometry was measured using digital imaging software (Leica IM50) and photographs taken using light microscopy. Three independent sperm traits were directly measured (flagellum, head and straight midpiece lengths) and three composite traits were calculated (total length, flagellum∶head and midpiece∶flagellum length ratios) to the nearest 0.1 µm (for more details see  and ). In order to minimize autocorrelation between predictor variables, independent and composite sperm traits were tested separately (note that independent traits were not correlated with each other, r<|0.17| and p>0.2). Although five sperm per male are generally used to describe sperm morphometry in passerine birds (), we included all sampled males in the analysis irrespective of number of sperm measured each (mean = 7 sperm per male, range = 1 to 10; see Fig. S2) since this species has low intra-male variation (Table S2), reasonable intra-male repeatability (; Table S2), and a single sperm captures c. 70% of this intra-male variation (see ; Fig. S3). In fact, the superb fairy-wren shows a two fold difference between inter- and intra-male coefficient of variation for sperm length, one of the highest for which comparable variation indices are available (inter∶intra CV ratio = 1.9; range in 26 species = 0.8 to 2.1; –). Nonetheless, we conservatively weighted all analyses by sampling effort category (low weight given to males with fewer than five sperm measured). Although we cannot report across-year repeatability in sperm traits, it is unlikely that sperm sampling restriction to 2005 would significantly confound our results for the following three reasons. First, in contrast to the only study that has shown environmentally-induced plasticity in sperm morphology in a passerine , there is no evidence that primary sexual traits and hormonal profiles at the time of sperm production differ across males of different social status or social group in this species (e.g. , ). Second, we found no variation in any sperm morphometric trait with respect to age, status or group composition at the time of sampling (MANOVA, p>0.2, Table S3). Third, evidence from a two passerine species suggests considerable within-individual across-year repeatability (Agelaius phoeniceus, S Lüpold, pers. comm.; Troglodytes aedon, E. Cramer, pers. comm.). Nonetheless, all analyses were repeated with the subsample of males with at least 5 sperm measured (see Table S4).
Finally, the inclusion of males that are potentially still reproductively active past the 2008–9 breeding season, the last year we have completed paternity assignment data (n = 20/59 and n = 17/47 males for extrapair and within-pair success, respectively) create a further and potentially confounding factor in our sample. We highlight the possibly biased data points in the Figures and provide the results using a restricted dataset excluding those males in Table S4. Model simplification was achieved using stepwise removal of terms and comparison of alternative models' fit using likelihood ratio tests . Quasibinomial error structure was used in cases where overdispersion needed to be accounted for . All analyses were conducted in R version 2.10.1 (R Development Core Team). Effect sizes and their 95% confidence intervals  were calculated for each alternative data subset's minimal adequate final models, based on the standardized variable methods proposed by  (see Table S5).
After controlling for the positive effect of male breeding experience (i.e. breeding season number), success at siring extrapair offspring declined with both flagellum length (Fig. 1; GLM with quasibinomial errors; season number: estimate (± s.e.) = 0.98±0.27, z = 3.64, p = 0.0006; flagellum length: estimate (± s.e.) = −0.67–44.83±16.56, z = −2.71, p = 0.009; n = 59 males) and flagellum∶head length ratio (Fig. 1; GLM with quasibinomial errors; season number: estimate (± s.e.) = 0.90±0.26, z = 3.43, p = 0.001; flagellum∶head ratio: estimate (± s.e.) = −18.59±9.23, z = −2.01, p = 0.049, n = 59 males). Therefore, cuckolding success was associated with sperm with a shorter flagellum and relatively larger heads. The alternative negative binomial GLM method shows these results are robust (Table S4). Removal of males that could be reproductively active past the end date for paternity data (n = 12 males who could change from low to high extrapair success late in their lives), did not qualitatively change the results (flagellum length p = 0.018; flagellum∶head ratio p = 0.048; Table S4 and Table S5). The same was true for the restriction of the dataset to males with at least five measured (Table S4 and Table S5).
(A) flagellum length and (B) relative length of flagellum to head section. Extrapair paternity success was transformed into a categorical variable based on the observed binomial frequency distribution peaks of fledged extrapair sired young (low = one or fewer, high = two or more). Males that are potential active breeders past the date of the current paternity assessment (2008/9 season) are represented by the open circles (n = 20 of the total n = 59 males). Fitted curves were calculated using the regression estimates from fitted models (male breeding experience included as a covariate).
In contrast, males with sperm with a longer flagellum and relatively shorter heads were more successful at preventing cuckoldry (Fig. 2; binomial GLMMs with male identity as a random factor; flagellum length: estimate (± s.e.) = 20.08±8.31, z = 2.42, p = 0.016; flagellum∶head ratio: estimate (± s.e.) = 11.50±4.31, z = 2.67, p = 0.008; n = 255 broods assigned to 47 males). The number of helpers did not affect within-brood paternity success in this sample (p>0.2). Although our measure of cuckolding avoidance (proportion of fledgling sired in all assigned nests) can be biased either way by future potential breeding attempts (n = 17 dominant males alive past the last available paternity analysis), removal of these data points did not change any of the previous results (flagellum length p = 0.03; flagellum∶head ratio p = 0.008). Note, however, that restriction of the dataset to males with at least five measured sperm considerably decreased effect size estimates (c. by one third) and rendered the results not significant for either sperm trait (Table S4 and Table S5).
(A) flagellum length and (B) relative length of flagellum to head section. Within-pair reproductive success is measured as the proportion of young at a social dominant male's nest(s). Analyses were conducted using male identity as a random factor (i.e. mixed models). Plots refer to mean paternity for all broods sampled. Males that are potential active breeders past the date of the current paternity assessment (2008/9 season) are represented by the open circles (n = 17 of the total n = 47 males). Fitted curves were calculated using the regression estimates from non-mixed effects fitted models.
Midpiece size (absolute or relative to flagellum length) was not associated with either extra- or within-pair reproductive success (all models, p>0.4; see Table S4). In summary, the same sperm morphometric traits have opposite effects for cuckoldry success and cuckoldry avoidance.
We found evidence of opposite selection on male sperm traits in superb fairy-wrens, a species under intense sperm competition. Male fairy wrens with shorter flagella and relatively larger heads sired more extrapair offspring, but were less likely to secure paternity at their own nest than males with the opposite sperm phenotype. To our knowledge, this is the first evidence for a naturally occurring selective trade-off for sperm morphology in a wild population.
Sperm size, design and numbers are known to influence the outcome of sperm competition (e.g. reviewed in , , ). Fertilization efficiency of sperm is a complex trait that is further influenced by female (cryptic) choice processes, often mediated by ejaculate storage , . Although we currently lack data on female fairy-wren sperm storage morphology, we can speculate on possible mechanisms that explain the association between sperm design and reproductive success based on what we know about (i) the likely sperm competition processes operating in this species, (ii) the differences in the timing and number of copulations between the pair and the extrapair mate, and (iii) the unique features of passerine sperm morphology.
Sperm competition context
The mating system of fairy-wrens theoretically generates a sperm competition scenario that is analogous to a random role (i.e. no strict assignment of males to the offense or defense position in the interaction), possibly loaded raffle (i.e. one male's sperm is devalued), with negligible sperm limitation, and very high risk of sperm competition for both the extrapair and the pair male (e.g. ). Females seek extrapair copulations regardless of the quality of their own mate , and unless paired to their son, always copulate with the social pair male as well (Cockburn & Double, unpublished data). Moreover, the high rate of sperm production , , large testes and cloacal protuberance –, relatively low breeding synchrony and hence intensity of extrapair matings , , and similar levels of circulating testosterone and sperm reserves between dominant males during the breeding season , , suggest that sperm limitation may be negligible. In this scenario, males are theoretically predicted to invest equally in sperm numbers irrespective of their perceived role (i.e. offense or defense) at the time of copulation –. Therefore, we can assume that adaptive ejaculate allocation (e.g. reviewed in ) is unlikely to influence sperm competition outcome in this species. Moreover, the strong directional selection on a honest phenotypic signal (nuptial plumage moult date, e.g. ) suggests that sperm competition processes based on genetic (in)compatibility ‘loaded raffle’ (e.g. ) are also an unlikely confound in this system. In addition, the timing of extrapair copulations is fairly consistent across females (see below).
Timing of mating
Rival males copulate at the same time relative to ovulation. Extragroup copulations are always sought by females three days prior to egg laying . Pair males follow their females closely on her return from the foray, and they copulate with her within half an hour of her return (Cockburn & Double, unpublished data). Thereafter, males show little interest in mate-guarding and/or copulation, and instead spend large part of the day displaying to females on neighbouring territories . Potential ‘loading’ in such sperm competition raffle is thus intimately associated with the relative timing of copulation, with the extragroup male always first to inseminate the female. Paradoxically, when discussing sperm-female interactions mechanisms at the proximate level (see below), the extrapair and pair males exert the roles of paternity defender and offender, respectively: the within-pair male tries to overcome the extrapair male's previously stored ejaculate. At the ultimate level, securing within-pair paternity in species with paternal care is inherently a defensive fitness strategy.
Sperm morphology and female-sperm interactions
Sperm design, i.e. the relative lengths of sperm components rather than total length or absolute component size, might be important target of selection, especially when they provide better correlates of sperm function . For instance, the relative size of the head to the flagellum (i.e. drag vs. power) was proposed  and later found (, , , but see ) to be a good predictor of sperm velocity in birds: sperm with higher flagellum∶head ratios swam faster, although this relationship did not hold at the intraspecific level in three promiscuous species (Agelaius phoeniceus, ; Quelea quelea, ; Tachycineta bicolor, ). Nevertheless, the pre- and post-copulatory scenarios where selection acts in our study species are quite different (e.g. EPC-seeking by all females and lack of precopulatory pair choice; see Introduction) from the more ‘traditional’ mating ecology of the three other promiscuous species aforementioned. More data are therefore needed. The relationship between flagellum and midpiece lengths has also been considered an important trait, associated with sperm energetic dynamics and sperm function –. It is interesting to note that in the present study neither absolute midpiece length nor its ratio to flagellum length were found to be associated with either type of paternity success. However, (relative) midpiece size is not as closely linked to sperm function in passerine birds (, , , cf. ) as in chickens or mammals (e.g. , ), which might explain its lack of relationship with fitness.
Passerine sperm are stored in sperm storage tubules (SSTs), located at the utero-vaginal junction of the female reproductive tract, with their heads facing the distal blind-ended part , . Across bird species, sperm total length generally increases with sperm competition level, but the latter relationship is the indirect result of a stronger sperm-length-SST-length correlation . In fact, the role of sperm-female interactions in the evolution of male gametes is clear and well supported empirically (reviewed in ). The most detailed histological study of SSTs in another highly promiscuous passerine, the alpine accentor, Prunella collaris , found no evidence for contractile elements, which would provide direct female anatomical control mechanisms over sperm access to and persistence in the SSTs. Therefore, although we can not rule out possible biochemical processes, it is not unrealistic to assume that features of passerine sperm themselves might strongly influence access to and endurance within the SSTs. For instance, relatively longer heads (e.g. more twists in the helix) might improve a sperm's resistance to passive loss from the SSTs, thus benefiting the extrapair male. Sperm with a longer flagellum and relatively shorter heads are predicted to have higher thrust forces and reduced drag , , often explaining the associated higher in vitro velocity . However, it is unclear how higher thrust benefits sperm in the offense capacity, since (i) sperm velocity and flagellum∶head ratio are not correlated across males of promiscuous species (see Introduction), (ii) there is no evidence for active sperm displacement in birds , , and (iii) it has been shown that avian sperm are passively transported from the SSTs to the site of fertilization, the infundibulum . We can speculate that more powerful and/or energy efficient (if not faster) morphometry might increase the proportion of within-pair male sperm that enter the SSTs, or perhaps those SSTs placed higher in the reproductive tract, and thus closer to the site of fertilization, or reduce the rate at which sperm are lost from the SSTs, as was observed in the domestic fowl (Gallus g. domesticus, ).
The short sperm of Maluridae
The evolution of sperm morphology in a within-species context has been the focus of several empirical (e.g. reviewed in ) and theoretical studies (e.g. , , ). For instance, longer sperm increase the competitive potential of an ejaculate or promote female sperm choice because longer sperm may, among other reasons, swim faster, live longer, be more effective in sperm displacement within the female reproductive tract, or indicate higher male quality . On the other hand, shorter sperm might be favored under raffle processes if the same number of sperm can be invested into an ejaculate for reduced energetic (and/or spatial) cost (e.g. ). Recent theoretical and comparative work suggest that the typical mode of sperm competition in birds follows raffle principles , , and the latter also applies to fairy-wrens (see above). Cuckolding success (cf. avoidance) is likely to be the major cause of male differential reproductive success in this species (cf. other passerines; e.g. ) since: (i) it is mostly under female control , (ii) a very small percentage (4–5%) of males sire the majority of extrapair offspring in the population (33–47% , this study), (iii) most dominant males suffer some within-pair paternity loss (77–95% broods have at least one extrapair chick , this study) and one third of males sire none of the young raised on their territory (13/46, 38%; this sample), (iv) subordinate male direct fitness is mostly derived through extragroup cuckoldry, particularly as they never mate with their mother , , . Since shorter sperm was positively associated with greater cuckolding success, this might explain why Maluridae has relatively shorter sperm than expected for their body and relative testes sizes . Moreover, producing shorter sperm might be a consequence of the selection for higher proportion of sperm producing tissue  in already space-constrained testes.
The observed antagonistic selection forces acting on superb fairy-wren sperm morphology provide a feasible mechanism for maintenance of some morphological variation under extreme postcopulatory sexual selection and preclude the existence of a universally favorable sperm phenotype at any given breeding season (cf. sexually-selected sperm hypothesis; ). Moreover, this study attests to the value of using well-documented, long-term study systems to improve our understanding of sperm competition evolutionary processes in natural conditions. We recommend that future work on this, and other (promiscuous) wild taxa should focus on proximate level enquiries, including female reproductive morphology and the genetic basis and covariation between sperm form and function.
Frequency distribution of number of extrapair offspring per male.
Frequency distribution of the number of sperm measured per male.
Assessment of the accuracy of individual sperm morphometry estimates obtained using different numbers of sperm per male.
Review of the evidence for paternity success trade-offs in male (pre-copulatory) phenotype in birds.
Repeatability of sperm measurements.
Effects of status, age and group type in sperm morphometrics.
We thank all members of the A. Cockburn research group that helped with life history data collection, L. Fromhage and A. Webster for valuable comments on earlier versions of the manuscript, and S. Nakagawa for statistical advice.
Conceived and designed the experiments: SC TRB AC. Performed the experiments: SC MCD NM. Analyzed the data: SC. Contributed reagents/materials/analysis tools: TRB AC. Wrote the paper: SC TRB AC.
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