Initiation of RNA polymerase (Pol) II transcription requires assembly of the pre-initiation complex (PIC) at the promoter. In the classical view, PIC assembly starts with binding of the TATA box-binding protein (TBP) to the TATA box. However, a TATA box occurs in only 15% of promoters in the yeast Saccharomyces cerevisiae, posing the question how most yeast promoters nucleate PIC assembly. Here we show that one third of all yeast promoters contain a novel conserved DNA element, the GA element (GAE), that generally does not co-occur with the TATA box. The distance of the GAE to the transcription start site (TSS) resembles the distance of the TATA box to the TSS. The TATA-less TMT1 core promoter contains a GAE, recruits TBP, and supports formation of a TBP-TFIIB-DNA-complex. Mutation of the promoter region surrounding the GAE abolishes transcription in vivo and in vitro. A 32-nucleotide promoter region containing the GAE can functionally substitute for the TATA box in a TATA-containing promoter. This identifies the GAE as a conserved promoter element in TATA-less promoters.
Citation: Seizl M, Hartmann H, Hoeg F, Kurth F, Martin DE, Söding J, et al. (2011) A Conserved GA Element in TATA-Less RNA Polymerase II Promoters. PLoS ONE 6(11): e27595. doi:10.1371/journal.pone.0027595
Editor: Yamini Dalal, National Cancer Institute, United States of America
Received: June 30, 2011; Accepted: October 20, 2011; Published: November 16, 2011
Copyright: © 2011 Seizl et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants of Deutsche Forschungsgemeinschaft (SFB646, TR5, FOR1068, NIM); the European Molecular Biology Organization (EMBO); the Boehringer Ingelheim Fonds to MS, the Elite Network of Bavaria to MS and FH; the LMUinnovativ project Bioimaging Network (BIN), an Advanced Investigator Grant of the European Research Council, and an LMUexcellent research professorship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
In eukaryotes, transcription of protein-coding genes relies on RNA polymerase (Pol) II, the general transcription factors (GTFs) TFIIB, -D, -E, -F, and –H, and coactivators such as Mediator and the Spt-Ada-Gcn5 acetyltransferase complex (SAGA) , . During activation, gene-specific transcription factors recruit coactivator complexes and TFIID, thereby facilitating pre-initiation complex (PIC) formation at the promoter. A subunit of TFIID, the TATA box-binding protein (TBP), binds the TATA box, which is located upstream of the transcription start site (TSS), and nucleates PIC assembly , . However, TATA boxes with the consensus sequence TATAWAWR occur in only 13% of yeast promoters , and 10% of human promoters .
Since TATA-less promoters require TBP for function , , , and bind TFIID , alternative pathways for PIC assembly were proposed , . In metazoans, many TATA-less promoters contain a downstream promoter element (DPE) and an initiator (INR), which interact with components of TFIID to facilitate PIC assembly , . Although the GTFs are highly conserved throughout eukaryotes, alternative core promoter elements could not be identified in yeast. A TATA box is not strictly required for TFIID-dependent activity of a yeast model promoter . Indeed, recent studies of the TATA-less yeast RPS5 promoter showed that functionally redundant AT-rich stretches within the core promoter region promote TFIID-dependent transcription .
Based on these observations, we hypothesized that TATA-less core promoters contain DNA elements that are functionally similar to the TATA box in promoting PIC assembly and Pol II transcription. Here we used a combination of bioinformatics, in vivo reporter gene assays, and in vitro biochemistry to identify and functionally characterize a region in TATA-less yeast core promoters that is bound by TBP, required for Pol II transcription and contains a novel conserved promoter element, the GA element, or GAE.
Many TATA-less yeast promoters contain a conserved GAE
In contrast to higher eukaryotes, where the distance between the TATA box and the TSS is fixed at around 30 nucleotides, a variable distance of 40−120 nucleotides is observed in yeast, apparently due to a TSS scanning mechanism . This has hampered bioinformatic discovery of core promoter elements other than the TATA box in yeast. To systematically search for a core promoter motif that could be functionally similar to the TATA box in TATA-less yeast promoters, we defined four criteria: (1) The motif should peak within a core promoter window of −110 to −50 nucleotides relative to the TSS. (2) The motif occurrence should be anti-correlated to the TATA box. (3) The motif should be frequent in this region in TATA-less promoters. (4) The motif should be highly conserved.
For each of the 1024 possible 5-mers, we calculated the frequency of occurrence within the core promoter window and the Matthews correlation coefficient with the TATA box (consensus TATAWAWR). This search identified a DNA element comprising one guanine followed by four adenines (GAAAA) as being most highly anti-correlated to the TATA box (Figure 1A, criterion 1 and 2). We refer to this novel element as GA element (GAE). It displays the second highest frequency of occurrence in the core promoter window (Table S1), only trumped in frequency by a subsequence of its longer version GAAAAA (criterion 3). We next analyzed the conservation of all 5-mers in the core promoter window among five closely related yeast species (Sc, S. paradoxus, S. mikatae, S. kudriavzevii, S. bayanus). We allowed for a shift of up to +/− 3 nucleotides between motifs in the multiple sequence alignments. Again, the GAE stands out as being the most highly conserved of all the more frequent 5-mers (Figure 1B, criterion 4). Its degree of conservation is slightly higher than that of the full and partial TATA box consensus (TATAWAWR and TATAA) and only a little lower than the more rare consensus binding sites of transcription factors Reb1 (ACCCG) and Mbp1 (ACGCG). Therefore, the GAE meets all four criteria defined above.
(A) Anti-correlation between different 5-mers and the TATA box in Sc core promoters. GAE is marked by a green triangle. (B) Frequency of DNA 5-mers and their conservation between five closely related yeast species (Sc, S. paradoxus, S. mikatae, S. kudriavzevii, S. bayanus) in the core promoter window (−110 to −50 relative to TSS) of 3711 Pol II promoters. For comparison, TATA consensus (TATAWAWR) and GAE-related sequences are shown. TATA-related sequences are marked by a purple triangle, GAE-related sequences are marked by a green triangle, transcription factor binding sites are marked by a blue square, and other 5-mers are marked by an open circle. (C) Percentage of Sc core promoters containing the TATA box, GAE, both elements or none of the elements with and without conservation between five closely related yeast species (left and right column, respectively). (D) Occurrence of the TATA box and GAE in 3711 Sc Pol II promoters aligned to the TSS. Core promoter window used for bioinformatics analysis is highlighted by a blue box.
Sc promoters containing only a GAE are three times more abundant than promoters containing only a TATA box (34% vs. 11.7%, respectively, Figure 1C). When filtered by conservation between the five closely related yeast species, 12.6% of promoters still contained the GAE, whereas 6.8% contained a TATA box (Figure 1C). The GAE is 3.4 times more frequent than expected from a mononucleotide background model calculated on the same region. This exceeds the TATA box and an A-stretch of length five, which are 3.3 times and 2.0 times more frequent, respectively. The presence of a GAE is anti-correlated with the presence of a TATA box (Figure 1C). Only 0.4% of all promoters contain both elements (Figure 1C). The GAE can be present in more than one copy in the core promoter region (Figure S1) and can contain additional adenines at the 3’end (Figure S2). When promoters are aligned to the TSS, the GAE peaks in the same region as the TATA box (Figure 1D). The similar position of the TATA box and the GAE with respect to the TSS, and the mutual exclusion of these two elements from core promoters suggested a functional similarity of both elements during PIC assembly.
A TATA-less yeast promoter that functions in vitro
To functionally characterize the GAE in vivo and in vitro, we chose the Sc TMT1 promoter, a TATA-less promoter with a single GAE in the core promoter region. For comparison, we used the Sc HIS4 promoter, which contains a TATA box and is widely used for in vitro transcription assays , , , . Both TMT1 and HIS4 are involved in amino acid biosynthesis and are regulated by the activator Gcn4. We tested both promoters in a β-galactosidase reporter gene assay, which measures promoter strength in vivo. Both promoters were active under standard growth conditions (Figure 2A). We next examined whether the promoters also supported Gcn4-activated transcription in vitro. Indeed, not only the TATA-containing HIS4 promoter but also the TATA-less TMT1 promoter was active (Figure 2B, lanes 2 and 5). Promoter activity depended on Pol II, since the Pol II-specific inhibitor α-amanitin abolished transcription (Figure 2B, lanes 3 and 6). These results establish the TMT1 promoter as the first native TATA-less promoter in yeast that is active in an activator-dependent in vitro transcription assay.
(A) Promoter activity in an in vivo β-galactosidase reporter gene assay of HIS4 and TMT1 Sc promoters. Negative control is background signal of the reporter plasmid without a promoter. (B) Promoter activity in an in vitro transcription assay of Sc HIS4 and Sc TMT1 promoter using yeast nuclear extracts. In vitro transcription was carried out with and without recombinant Gcn4 activator. To test Pol II specificity of transcription, 0.04 mM α-amanitin was added. Specific transcripts from the HIS4 TSS and TMT1 TSS are marked by a and b, respectively.
The GAE-containing region is required for promoter function
To test whether the GAE is required for TMT1 promoter function, we generated several promoter mutants (Figure 3A) and tested their activities in β-galactosidase reporter gene assays. Deletion of the bioinformatically defined 5-nucleotide GAE had a moderate negative effect on promoter activity (Figure 3B, lane 2). Whereas replacing the 5-nucleotide GAE with GGCCG had no apparent defect (Figure 3B, lane 3), mutating the 12-nucleotide GAE-containing region (four and three nucleotides up- and downstream, respectively) strongly impaired promoter activity (Figure 3B, lane 4). Screening of additional promoter mutants revealed a strong dependence of promoter function on the four nucleotides directly upstream of the GAE (Figure 3B, lane 9).
(A) Schematic depiction of the TATA-less TMT1 and the TATA-containing HIS4 promoter. TATA box and bioinformatically defined GAE are marked by a black box. The 12-nucleotide GAE-containing region is marked by a dashed line. Promoter mutants are shown below the sequences. Activity of each variant in in vivo β-galactosidase reporter gene assays is shown (wildtype activity (++), decreased activity (+) and severely decreased activity (−)). (B) Determination of the core promoter region required for TMT1 promoter function. Activities of wild type TMT1 and several mutants in the GAE-containing region in an in vivo β-galactosidase reporter gene assay are shown. Negative control is background signal of the reporter plasmid without a promoter. (C) Promoter activities in an in vitro transcription assay using a nucleosome-free DNA template and yeast nuclear extracts.
Activity of the TMT1 promoter was also strongly decreased when the guanines in the 12-nucleotide GAE-containing region were changed to adenines (Figure 3B, lane 10), generating a poly(dA) stretch. A similar defect in promoter activity was observed in activated transcription assays using a nucleosome-free DNA template and yeast nuclear extracts (Figure 3C) in vitro. Thus the GAE-containing region is functionally distinct from previously described poly(dA) stretches, which are proposed to occlude nucleosomes from promoters , . These results suggest that the role of the GAE-containing region is not related to nucleosome-dependent promoter accessibility. The defects of the GAE deletion, the mutations in the flanking regions, and the polyA mutation suggest that TMT1 promoter function is strongly dependent on the sequence context in the GAE-containing region.
A TATA-less promoter is bound by TBP
Because the GAE and the TATA box occur at similar distances from the TSS, we tested whether the GAE-containing promoter region binds TBP. We used an electrophoretic mobility shift assay (EMSA) and fluorescently labeled DNA encompassing 40 base pairs of HIS4 and TMT1 promoter DNA. TBP bound HIS4 DNA as expected, but also bound the TATA-less TMT1 DNA (Figure 4A, lane 2 and 8). Both promoter DNAs could also form a stable complex with TBP and TFIIB (Figure 4A, lane 3 and 9). The observed binding was specific to double stranded DNA (Figure S3). As expected, TBP and TFIIB binding was strongly decreased by mutating the TATA box of HIS4 (Figure 4A, lane 5 and 6). Mutating the 12-nucleotide GAE-containing region also impaired TBP and TFIIB binding (Figure 4B, lane 5 and 6). TBP and TFIIB binding was not affected when guanines in the 12-nucleotide GAE-containing region were replaced with adenines (Figure 4B, lane 8 and 9). These results show that a stretch of adenines is sufficient for TBP binding, but not for promoter activity. The guanines in and around the GAE thus appear to have a function distinct from TBP binding. This is consistent with the observation that TBP binding does not necessarily correlate with promoter activity , .
(A) and (B) Electrophoretic mobility shift assay. A 5’-Cy5-labeled 40 bp double stranded DNA of the respective promoter construct was incubated with recombinant TBPcore alone or TBP core and TFIIB. The protein-DNA complex was separated from free DNA by native polyacrylamide gel electrophoresis. Free DNA and bound protein-DNA complexes are indicated.
The GAE-containing region functionally substitutes for the TATA box
To further investigate the potential functional similarity of the GAE-containing region and the TATA box, we performed promoter substitution experiments in vivo and in vitro. We found that the HIS4 TATA box can functionally replace the 12-nucleotide GAE-containing region in the TMT1 promoter (Figure 5A, lane 3). This substitution does not impair binding of TBP and TFIIB (Figure S4A, lanes 5 and 6). In contrast, the 12-nucleotide GAE-containing region is neither sufficient for functional replacement of the TATA box in the HIS4 promoter (Figure 5A, lane 6) nor for binding of TBP and TFIIB (Figure S4B, lane 5 and 6). Similar observations were made when replacing the TATA box in the SER3 yeast promoter by the 12-nucleotide GAE-containing region (Figure S4C).
(A) Schematic depiction of promoter substitution constructs of the TATA-less TMT1 and the TATA-containing HIS4 promoter. TATA box and bioinformatically defined GAE are marked by a black box. Solid and dashed lines represent HIS4 and TMT1 promoter sequences, respectively. TSS of HIS4 and TMT1 are labeled with a and b, respectively. Half-arrows mark primer annealing sites for primer extension reaction. (B) Promoter activity in an in vivo β-galactosidase reporter gene assay and in vitro transcription (txn) assay of the wild type (lane 1 and 4) and mutated TMT1 and HIS4 promoter. Mutations as indicated in Figure 5A. Negative control (lane 7) is background signal of the reporter plasmid without a promoter. (C) Promoter activity in an in vivo β-galactosidase reporter gene assay and in vitro transcription (txn) assay of the wild type (lane 1 and 3) and promoter substitution constructs as indicated in Figure 5A. Negative control (lane 6) is background signal of the reporter plasmid without a promoter.
To determine a minimal region in the TMT1 promoter that could substitute part of the HIS4 promoter without loss of activity, we tested several HIS4-TMT1 fusion constructs (Figure 5B). We found that the TMT1 core promoter fused to the HIS4 upstream activating sequence is active both in vivo and in vitro (Figure 5C, lane 4). This suggests that regions surrounding the 12-nucleotide GAE-containing region are required for function. Indeed, inserting a 32-nucleotide GAE-containing region (14 and 13 nucleotides up- and downstream of the GAE, respectively) could maintain partial HIS4 activity (Figure 5C, lane 5). Including even longer up- or downstream TMT1 sequences did not increase activity (Figure S5). This is consistent with our observation that sequence context is critical for TMT1 activity (Figure 3B). This indicates that the AT-rich sequences flanking the GAE in the TMT1 promoter are also important for activity.
Here we describe a novel core promoter element in yeast, the GA element, or GAE, which is found almost exclusively in TATA-less promoters. Similar to TATA-containing promoters, the GAE-containing TMT1 promoter is bound by TBP and supports formation of a TBP-TFIIB-DNA complex. Together with the anti-correlation with canonical TATA boxes, this suggests a functional similarity of the TATA box and the GAE-containing region. The mutual exclusion of TATA box and GAE appears not to be the result of an inhibitory role of GAE. Insertion of the GAE up- or downstream of the HIS4 TATA box had no effect on promoter function (Figure S6). We demonstrate that TMT1 promoter function is highly dependent on sequence context. The GAE-flanking region and the guanine residues in the 12-nucleotide GAE-containing region are crucial for promoter function. Similarly, previous studies have shown that also TATA box function and TBP-TATA binding is influenced by TATA-flanking sequences . In contrast to the TATA box, the GAE can be present in more than one copy in a promoter. Similarly, a study of the TATA-less RPS5 promoter demonstrated the presence of multiple, functionally redundant AT-rich sequences, which were shown to recruit TFIID .
Recent genome-wide studies indicated that activation of TATA-less genes is dominated by TFIID , . Consistently, a detailed study of the TATA-less TUB2 promoter had demonstrated a strong TFIID dependence . TUB2 activity was severely reduced in temperature-sensitive TFIID mutant backgrounds. Insertion of a canonical TATA box at -55 relative to the TSS could alleviate this defect and restore promoter function. Intriguingly, when we inspected the TUB2 sequence, we found a conserved GAE at the exact point of insertion. This is consistent with our finding that the GAE is mainly found in TFIID-dominated promoters (Figure S7).
Several studies of poly(dA:dT) tracts in individual model promoters indicated a role in promoter function that is linked to nucleosome positioning , , , , . Further, a recent theoretical genome-wide study described G/C-capped poly(dA:dT) tracts associated with the nucleosome-free region of TATA-less promoters in yeast . These tracts were suggested to define the center of the nucleosome-free region. However, a distinction of a direct effect on nucleosome positioning and indirect effects, such as recruiting chromatin remodelers or the transcription machinery, could not be made. Our in vitro transcription experiments done on a nucleosome-free DNA template demonstrate a direct role of the GAE-containing region in transcription, and suggest a function independent of nucleosome positioning. Consistent with our findings, the deletion of a poly(dA:dT) tract in the ILV1 promoter decreased transcription significantly without affecting nucleosome organization in vivo . Since the GAE described here appears to overlap with a part of the previously described poly(dA:dT) tracts, those tracts that fall in the described core promoter window may be redefined as GAE.
Materials and Methods
The TSS used for our analysis were generated by merging the cDNA data set of Miura et. al  and the 5’-SAGE data of Zhang and Dietrich . In both sets the TSS was considered to be the position with the highest tag count in a window of at most 300 bp upstream of a gene. If a gene has tag counts in both data sets, the TSS of the Miura set was used. The resulting data set consists of 3711 TSSs for annotated ORFs. For co-occurrence analysis, the Matthews Correlation Coefficient (MCC) was calculated for all 5-mers with respect to the TATA box consensus site (TATAWAWR) using the following formula.where TP stands for regions with both the 5-mer and the TATA box present, TN stands for regions with no motif present, FP stands for regions with only the TATA box present, and FN stands for regions with only the 5-mer present. Only occurrences were used that started in the TATA box region, i.e. −110 to −50 relative to the TSS. The conservation score was then calculated as the mean number of conserved nucleotides per motif position. This score ranges between 0 (all positions are mutated) and 4 (every motif position is conserved in all four related species), since five related yeast species (Sc, S. paradoxus, S. mikatae, S. kudriavzevii, S. bayanus) were used. For calculating the number of mutations, the start position in a window of +/−3 bp with the least number of mutations to the motif in Sc in every species was used.
Reporter gene assay
The native promoter sequences of HIS4 (428 bp upstream to 24 bp downstream of the start codon) and TMT1 (273 bp upstream to 24 bp downstream of the start codon) and their mutant variants were cloned between HindIII/BamHI into a pRS315 plasmid with the lacZ gene inserted between NotI/SalI. Reporter plasmids were transformed into BY4741 wild type yeast cells and grown in biological duplicates in SD (–leu) medium to an OD600 of 0.5–1.0. β-galactosidase levels were determined using the Yeast β-galactosidase Assay Kit (Thermo Scientific, #75768).
In vitro transcription
Nuclear extracts were prepared from 3 l of BY4741 wild type yeast culture as described (www.fhcrc.org/science/basic/labs/hahn) 33]. In vitro transcription and analysis by primer extension were performed as described 33]. Primer extension for all constructs was done using the same 5’-Cy5-labeled oligonucleotide (5’-TTCACCAGTGAGACGGGCAAC). For activated transcription 200 ng of recombinant full-length Gcn4 was added. Template plasmids were generated by inserting the respective promoter sequence as described above in pBluescript KS+ with HindIII and BamHI. The Sc Gcn4 gene was inserted into vector pET21a with NcoI and SacI introducing an N-terminal hexahistidine tag. Transformed E. coli BL21 (DE3) RIL cells (Stratagene) were grown in LB medium at 37°C to an OD600 of 0.5. Expression was induced with 0.5 mM IPTG for 16 h at 18°C. For protein purification, cells were lysed by sonication in buffer A (20 mM Tris/HCl pH 8.0 25°C, 500 mM NaCl, 5 mM DTT). After centrifugation, the supernatant was loaded twice onto a 2 ml Ni-NTA column (Qiagen) equilibrated with buffer A. The column was washed three times with 10 column volumes (CV) of buffer A containing 20 mM imidazole. Bound protein was eluted with 10 CV of buffer A containing 500 mM imidazole. Protein was further purified by cation exchange chromatography using a HiTrap SP column (GE Healthcare) equilibrated with buffer B (20 mM HEPES pH 7.5 25°C, 10% Glycerol, 1 mM EDTA, 2 mM DTT). The protein was eluted with a linear gradient of 10 CVs from 0 to 1 M NaCl in buffer B. After concentration, the sample was applied to a Superdex 200 size exclusion column (Amersham) equilibrated with buffer C (20 mM HEPES pH 7.5 25°C, 150 mM KAcetate, 10% Glycerol, 1 mM EDTA, 2 mM DTT). The sample was concentrated to approximately 0.5 mg/ml, flash frozen in small aliquots in liquid nitrogen, and stored at −80°C. All buffers contained a protease inhibitor cocktail.
Electrophoretic mobility shift assay
Electrophoretic mobility shift assays (EMSA) were performed as described 34], with minor modifications. Templates were generated by annealing the complimentary PAGE-purified oligonucleotides (coding strand was 5’-Cy5-labeled). The binding reaction contained 3 nM of 40 bp 5’-Cy-5-labeled dsDNA, 300 nM TBPcore and 3 µM TFIIB, 1 mM DTT, 50 µg/ml Heparin in 1x binding buffer (4% glycerol, 4 mM Tris-HCl pH 8.0 23°C, 60 mM KCl, 5 mM MgCl2, 100 µg/ml BSA, 0.1% Tween 20) in a total reaction volume of 20 µl. Recombinant TBPcore and TFIIB were expressed in E. coli and purified as described 35]. Proteins were diluted in dilution buffer (20 mM Tris pH 7.9 23°C, 150 mM KCl, 1 mM DTT, 10% Glycerol, 50 µg/ml BSA). Samples were incubated for 30 min at 18°C and loaded on a 6% native polyacrylamide gel (acrylamide:bisacrylamide 60∶1, 190 mM glycine, 25 mM Tris pH 8.3 23°C with acetic acid, 0.5 mM DTT, 0.1% APS, 1% TEMED). The gel was pre-run for five min and then run at 160V for 15 min at room temperature in 190 mM glycine, 25 mM Tris pH 8.3 23°C. Gels were analyzed with a typhoon scanner FLA9400 and ImageQuant Software (GE Healthcare). The oligos used for the assay had the following sequence (only coding strand is shown):
HIS4 (TATA), 5’-ACAGTAGTATACTGTGTATATAATAGATATGGAACGTTAT;
HIS4 (GC-8), 5’-ACAGTAGTATACTGTGGGCCGGCCAGATATGGAACGTTAT;
HIS4 (GAE), 5’-TAGTATACTGTGAAAGGAAAAGGAAGATATGGAACGTTAT;
TMT1 (GAE), 5’-ATTTTCATTTTTAAAGGAAAAGGAATGTAACTAATTTAGT;
TMT1 (GC-12), 5’-ATTTTCATTTTTGGCCGGCCGGCCATGTAACTAATTTAGT;
TMT1 (TATA), 5’-ATTTTCATTTTTTATATAATATGTAACTAATTTAGT;
TMT1 (A-12), 5’- ATTTTCATTTTTAAAAAAAAAAAAATGTAACTAATTTAGT
The GAE can occur in multiple copies. Fraction of sequences with the TATA box (purple) and GAE (green) in at least a given number of multiple occurrences per sequences in 3711 Sc promoters aligned at the TSS.
Frequency of the GAE with different length of A-tracts in 3711 Sc core promoters.
Only double stranded templates are bound by TBP and TFIIB. Electrophoretic mobility shift assay. A 5’-Cy5-labeled 40 bp single (ssDNA) and double stranded (dsDNA) templates were incubated with recombinant TBPcore alone or TBP core and TFIIB. The protein-DNA complex was separated from free DNA by native polyacrylamide gel electrophoresis. Free DNA and bound protein-DNA complexes are indicated.
GAE function depends on promoter context. (A) and (B) Electrophoretic mobility shift assay. A 5’-Cy5-labeled 40 bp double stranded DNA of the respective promoter construct was incubated with recombinant TBPcore alone or TBP core and TFIIB. The protein-DNA complex was separated from free DNA by native polyacrylamide gel electrophoresis. Free DNA and bound protein-DNA complexes are indicated. (C) Promoter activity in an in vivo β-galactosidase reporter gene assay and in vitro transcription (txn) assay of the wild type and mutated Sc SER3 promoter. The 8-nucleotide TATA box of the SER3 promoter was substituted by the 12-nucleotide GAE-containing region of the TMT1 promoter.
The GAE-containing region can functionally replace the TATA box. (A) Schematic depiction of promoter substitution constructs of the TATA-less TMT1 and the TATA-containing HIS4 promoter. TATA box and bioinformatically defined GAE are marked by a black box. Solid and dashed lines represent HIS4 and TMT1 promoter sequences, respectively. TSS of HIS4 and TMT1 are labeled with a and b, respectively. Half-arrows mark primer annealing sites for primer extension reaction. (B) Promoter activity in an in vivo β-galactosidase reporter gene assay and in vitro transcription (txn) assay of the wild type and mutated HIS4 and TMT1 promoter. Negative control (lane 8) is background signal of the reporter plasmid without a promoter. Specific transcripts from the HIS4 TSS and TMT1 TSS are marked by a and b, respectively.
GAE has no inhibitory effect on the TATA-containing HIS4 promoter. Promoter activity in an in vivo β-galactosidase reporter gene assay of the wild type and mutated HIS4 promoter. Promoter substitution constructs as indicated on the right. Negative control is background signal of the reporter plasmid without a promoter.
The GAE anti-correlates with SAGA-dominated genes. Percentage of Sc core promoters containing TATA box, GAE, both elements or none of the elements in SAGA- and TFIID dominated Sc promoter classes , .
All Sc promoters containing a bioinformatically defined GAE.
We thank the members of the Cramer laboratory for help and discussions. We thank Sarah Sainsbury for providing recombinant TBPcore and TFIIB. We thank Steve Hahn and Z.S. Juo for providing expression plasmids. We thank Claudia Gugenmus for preparation and processing of bioinformatics data. We thank Stefanie Steger and Martina Rami for help with cloning of template plasmids.
Conceived and designed the experiments: MS FH PC. Performed the experiments: MS FH FK. Analyzed the data: MS HH FH. Contributed reagents/materials/analysis tools: MS HH FH DM. Wrote the paper: MS HH FH JS PC.
- 1. Sikorski TW, Buratowski S (2009) The basal initiation machinery: beyond the general transcription factors. Current Opinion in Cell Biology 21: 344–351.
- 2. Roeder RG (1996) The role of general initiation factors in transcription by RNA polymerase II. Trends in Biochemical Sciences 21: 327–335.
- 3. Koleske AJ, Young RA (1994) An RNA polymerase-II holoenzyme responsive to activators. Nature 368: 466–469.
- 4. Ranish JA, Yudkovsky N, Hahn S (1999) Intermediates in formation and activity of the RNA polymerase II preinitiation complex: holoenzyme recruitment and a postrecruitment role for the TATA box and TFIIB. Genes & Development 13: 49–63.
- 5. Basehoar AD, Zanton SJ, Pugh BF (2004) Identification and distinct regulation of yeast TATA box-containing genes. Cell 116: 699–709.
- 6. Yang CH, Bolotin E, Jiang T, Sladek FM, Martinez E (2007) Prevalence of the initiator over the TATA box in human and yeast genes and identification of DNA motifs enriched in human TATA-less core promoters. Gene 389: 52–65.
- 7. Cormack BP, Struhl K (1992) The TATA-binding protein is required for transcription by all 3 nuclear-RNA polymerases in yeast-cells. Cell 69: 685–696.
- 8. Burley SK (1996) The TATA box binding protein. Current Opinion in Structural Biology 6: 69–75.
- 9. Pugh BF, Tjian R (1991) Transcription from a TATA-less promoter requires a multisubunit TFIID complex. Genes & Development 5: 1935–1945.
- 10. Hahn S, Buratowski S, Sharp PA, Guarente L (1989) Yeast TATA-binding protein TFIID binds to TATA elements with both consensus and nonconsensus DNA-sequences. Proceedings of the National Academy of Sciences of the United States of America 86: 5718–5722.
- 11. Smale ST (1996) Transcription initiation from TATA-less promoters within eukaryotic protein-coding genes. Biochimica et Biophysica Acta 1351: 73–88.
- 12. Chalkley GE, Verrijzer CP (1999) DNA binding site selection by RNA polymerase II TAFs: a TAF(II)250-TAF(II)150 complex recognizes the Initiator. Embo Journal 18: 4835–4845.
- 13. Juven-Gershon T, Hsu JY, Theisen JWM, Kadonaga JT (2008) The RNA polymerase II core promoter - the gateway to transcription. Current Opinion in Cell Biology 20: 253–259.
- 14. Singer VL, Wobbe CR, Struhl K (1990) A wide variety of DNA-sequences can functionally replace a yeast TATA element for transcriptional activation. Genes & Development 4: 636–645.
- 15. Sugihara F, Kasahara K, Kokubo T (2011) Highly redundant function of multiple AT-rich sequences as core promoter elements in the TATA-less RPS5 promoter of Saccharomyces cerevisiae. Nucleic Acids Research 39: 59–75.
- 16. Kuehner JN, Brow DA (2006) Quantitative analysis of in vivo initiator selection by yeast RNA polymerase II supports a scanning model. Journal of Biological Chemistry 281: 14119–14128.
- 17. Auble DT, Hansen KE, Mueller CGF, Lane WS, Thorner J, et al. (1994) Mot1, a global repressor of RNA-polyermase-II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism. Genes & Development 8: 1920–1934.
- 18. Muldrow TA, Campbell AM, Weil PA, Auble DT (1999) MOT1 can activate basal transcription in vitro by regulating the distribution of TATA binding protein between promoter and nonpromoter sites. Molecular and Cellular Biology 19: 2835–2845.
- 19. Lariviere L, Seizl M, van Wageningen S, Rother S, van de Pasch L, et al. (2008) Structure-system correlation identifies a gene regulatory Mediator submodule. Genes & Development 22: 872–877.
- 20. Struhl K (1985) Naturally-occuring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast. Proceedings of the National Academy of Sciences of the United States of America 82: 8419–8423.
- 21. Iyer V, Struhl K (1995) Poly(dA-dT), a ubiquitous promoter element that stimulates transcription via its intrinsic DNA-structure. Embo Journal 14: 2570–2579.
- 22. Kuras L, Struhl K (1999) Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme. Nature 399: 609–613.
- 23. Muller F, Demeny MA, Tora L (2007) New problems in RNA polymerase II transcription initiation: Matching the diversity of core promoters with a variety of promoter recognition factors. Journal of Biological Chemistry 282: 14685–14689.
- 24. Faiger H, Ivanchenko M, Cohen I, Haran TE (2006) TBP flanking sequences: asymmetry of binding, long-range effects and consensus sequences. Nucleic Acids Research 34: 104–119.
- 25. Huisinga KL, Pugh BF (2004) A genome-wide housekeeping role for TFIID and a highly regulated stress-related role for SAGA in Saccharomyces cerevisiae. Molecular Cell 13: 573–585.
- 26. Tsukihashi Y, Miyake T, Kawaichi M, Kokubo T (2000) Impaired core promoter recognition caused by novel yeast TAF145 mutations can be restored by creating a canonical TATA element within the promoter region of the TUB2 gene. Molecular and Cellular Biology 20: 2385–2399.
- 27. Thiryblaise LM, Loppes R (1990) Deletion analysis of the ARG4 promoter of Saccharomyces cerevisiae - a poly(dA-dT) stretch involved in gene-transcription. Molecular & General Genetics 223: 474–480.
- 28. Moreira JMA, Horz W, Holmberg S (2002) Neither Reb1p nor Poly(dA-dT) elements are responsible for the highly specific chromatin organization at the ILV1 promoter. Journal of Biological Chemistry 277: 3202–3209.
- 29. Graham IR, Chambers A (1994) A Reb1p-binding site is required for efficient activation of the yeast RAP1 gene, but multiple binding-sites for Rap1p are not essential. Molecular Microbiology 12: 931–940.
- 30. Wu R, Li H (2010) Positioned and G/C-capped poly(dA:dT) tracts associate with the centers of nucleosome-free regions in yeast promoters. Genome Research 20: 473–484.
- 31. Miura F, Kawaguchi N, Sese J, Toyoda A, Hattori M, et al. (2006) A large-scale full-length cDNA analysis to explore the budding yeast transcriptome. Proceedings of the National Academy of Sciences of the United States of America 103: 17846–17851.
- 32. Zhang ZH, Dietrich FS (2005) Mapping of transcription start sites in Saccharomyces cerevisiae using 5 ' SAGE. Nucleic Acids Research 33: 2838–2851.
- 33. Seizl M, Lariviere L, Pfaffeneder T, Wenzeck L, Cramer P (2011) Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization. Nucleic Acids Research 39: 6291–6304.
- 34. Auble DT, Hahn S (1993) An ATP-dependent inhibitor of TBP binding to DNA. Genes & Development 7: 844–856.
- 35. Kostrewa D, Zeller ME, Armache KJ, Seizl M, Leike K, et al. (2009) RNA polymerase II-TFIIB structure and mechanism of transcription initiation. Nature 462: 323–330.