Soil communities associated with specific plant species affect individual plants' growth and competitive ability. Limited evidence suggests that unique soil communities can also differentially influence growth and competition at the ecotype level. Previous work with Arabidopsis thaliana has shown that accessions produce distinct and reproducible rhizosphere bacterial communities, with significant differences in both species composition and relative abundance. We tested the hypothesis that soil communities uniquely affect the growth and reproduction of the plant accessions with which they are associated. Specifically, we examined the growth of four accessions when exposed to their own soil communities and the communities generated by each of the other three accessions. To do this we planted focal accessions inside a ring of six plants that created a “background” soil community. We grew focal plants in this design in three separate soil treatments: non-sterile soil, sterilized soil, and “preconditioned” soil. We preconditioned soil by growing accessions in non-sterile soil for six weeks before the start of the experiment. The main experiment was harvested after seven weeks of growth and we recorded height, silique number, and dry weight of each focal plant. Plants grown in the preconditioned soil treatment showed less growth relative to the non-sterile and sterile soil treatments. In addition, plants in the sterile soil grew larger than those in non-sterile soil. However, we saw no interaction between soil treatment and background accession. We conclude that the soil communities have a negative net impact on Arabidopsis thaliana growth, and that the unique soil communities associated with each accession do not differentially affect growth and competition of study species.
Citation: Aguilera AG, Colón-Carmona A, Kesseli R, Dukes JS (2011) No Accession-Specific Effect of Rhizosphere Soil Communities on the Growth and Competition of Arabidopsis thaliana Accessions. PLoS ONE 6(11): e27585. doi:10.1371/journal.pone.0027585
Editor: Mari Moora, University of Tartu, Estonia
Received: March 17, 2011; Accepted: October 20, 2011; Published: November 15, 2011
Copyright: © 2011 Aguilera et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the U.S. National Science Foundation, through a Graduate Research Fellowship to AGA and through grant IOS-0847691 to RK, JSD, ACC, and M. Shiaris. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Plant-soil feedbacks have received recognition as a driving mechanism behind plant abundance and rarity , . Plant species and even genotypes ,  supply soil communities with litter, decomposing roots and exudates that provide distinct combinations of substrates to unique microbial and macro-invertebrate communities , , . These soil communities influence nutrient cycling, plant nutrient availability, disease protection, plant health, and plant growth, thereby creating plant-soil feedbacks , , , , , . To date, research on plant-soil feedbacks has focused on successional dynamics and invasive species. A meta-analytical review by Kulmatiski et al.  provides support for the hypothesis that negative feedbacks among early successional species accelerate succession while positive feedbacks later in succession stabilize communities , , , . A growing body of evidence suggests feedbacks influence some plant invasions , , , . Reinhart and Callaway  showed the invasive plant Centaurea maculosa to have negative plant-soil feedbacks in its native soil, and positive feedbacks in soil from its invaded range in North America. The authors suggest that this escape from negative feedbacks and facilitation in the invaded range contributes to Centaurea's invasive success. The review by Kulmatiski et al.  revealed that, while positive and negative plant-soil feedbacks have been reported in the literature, the majority of feedbacks were negative (70%), and annuals experienced greater feedback responses than did perennial species. Feedback experiments often assume that microbial soil communities that exert negative impacts do so directly via pathogens or by limiting nutrient acquisition. However, microbes can also significantly limit plant productivity by competing with plants for nutrients , .
While there are several mechanisms for soil mediated effects on plant growth, there is a large body of evidence of direct, microbially-mediated host-specific plant-soil feedbacks. Bartelt-Ryser et al.  showed that host-specific soil communities persisted in the soil even after host species removal. Further support comes from research identifying specific soil microbial communities in the rhizosphere, the area where the soil microbial community is influenced by plant roots. For example, Cicer arietium, Brassica napus, and Sorghum bicolor each host a distinct community of Eubacteria in their rhizosphere . It has also been demonstrated that species respond uniquely to microbial inoculants. Westover and Bever  found that Anthoxanthum odoratum and Panicum sphaerocarpom responded differently to host-specific isolates of Bacillus mycoides. Each plant species preferred the bacteria cultivated by the other; Anthoxanthum had a more positive growth response to Bacillus cultivated on Panicum, and Panicum had a more positive response to Bacillus cultivated on Anthoxanthum. Molecular research has also determined that plant exudates can influence expression of genes in some plasmids . Clearly, exploration of the very complicated set of feedbacks at play in the rhizosphere has only just begun.
While the list of species-specific plant-soil feedbacks has grown rapidly, little effort has gone into detecting whether unique feedbacks exist for different natural variants (ecotypes) within a species. While potentially more subtle, it seems likely that such ecotype-specific feedbacks could be widespread; ecotypes themselves are genetically distinct, geographically separated populations that are uniquely adapted to their place of origin. Recent research shows that different genotypes within a plant species cultivate unique bacterial communities. Schweitzer et al.  have shown that intraspecific differences in microbial community composition between genotypes of Populus sp. are greater than interspecific differences. Using the model system Arabidopsis thaliana (hereafter referred to as Arabidopsis), Micallef et al.  discovered that different accessions (ecotypes) of Arabidopsis generate unique and reproducible bacterial communities in their rhizosphere. In addition, Bressan et al.  showed that a transgenic Arabidopsis plant line overproducing glucosinolates, secondary metabolites known for their antimicrobial properties, displayed distinct root exudate profiles and rhizobacterial communities (bacterial communities associated with plant roots). This implies that even slight genetic differences can significantly alter the chemistry of the rhizosphere and have a meaningful effect on the microbial communities associated with a given accession. Further, recent work by Biedrzycki et al.  describes how Arabidopsis is able to recognize self and kin (of same accession) plants via direct interaction with root exudates. They show that accessions exposed to a “stranger's” root exudates grew longer lateral roots than those that were exposed to “sibling” exudates. Different accessions of Arabidopsis have also been shown to have significantly different competitive effects and responses to competition with other accessions and species , . However, the extent to which this is due to plant morphology as opposed to soil feedbacks is unknown.
We hypothesized that accession specific soil communities would each have unique effects on plant growth and competition. We tested this hypothesis with four Arabidopsis accessions. We grew each alone (solo), as a focal plant grown with background competitors from its own accession (genetic monoculture), and as a focal with each of the other three accessions as background competitors. Competition experiments were replicated in soil that was sterile, non-sterile, or that had been “preconditioned” with Arabidopsis plants. We expected that focal plant growth would vary depending on the ecotype of background competitors. We also expected that the rhizosphere communities of the different competitors would differentially affect focal plant growth. We predicted that these plant-soil feedbacks would be negative. Further, we anticipated that intra- accession feedbacks would be the strongest, and that plants exposed to “other” accessions would exhibit a “release” from co-evolved microbial species. Due to the a limited amount of time for competing plants to establish and generate rhizosphere communities that are in contact with focal plant root systems, we anticipated that differences in soil communities' effects would be small. We expected the soil communities in the preconditioned soil to be established at the start of the experiment. Therefore, we expected the effect of competing accessions to be greatest in the preconditioned soil treatment. To determine the degree to which nutrient depletion affected plants in the preconditioned soil we performed a “follow-up” experiment that included a fertilization treatment of the preconditioned soil.
Arabidopsis thaliana is an annual weed in the Brassicaceae that is commonly used in genetic and molecular studies. Arabidopsis is an ideal species with which to study the impact of soil feedbacks on competition for two reasons. First, Micallef et al.  have shown that different accessions of Arabidopsis produce distinct and reproducible rhizosphere bacterial communities, with significant differences in both species composition and relative abundance. Second, Arabidopsis' small size and short life cycle allow for extensive replication and relatively short experimental durations.
We used four Arabidopsis accessions: Columbia (Col; USA), Cape Verde Islands (Cvi, Cape Verde), Landsberg erecta (Ler; Germany), and Rld (Rld-1; Russia). We selected these accessions to develop maximally different rhizobacterial communities as shown by Micallef et al. (2009). We sterilized all seeds in a 33% bleach solution, and thoroughly rinsed with sterile water. We then stratified all seeds in sterile water at 4°C for 48 hours before planting.
We grew a single “focal” plant of each accession in competition with the same ecotype and with each of the other three accessions (Fig. 1). We planted 12 “background” seeds in a ring around the edge of a 5 cm “cone-tainer” (Stuewe & Sons, Inc., Tangent, Oregon). We thinned background plants to 6 plants per pot a week after germination. One week after planting the background seeds, we planted 3 focal seeds in the center of each pot. After 5–7 days we thinned to 1 focal plant per pot. In addition we planted “solo” treatments with focal plants of each accession grown alone in a cone-tainer to determine the effect of competition (Fig. 1). We replicated the competition treatments 15 times, and the solo treatments 5 times, using a block design, with solo treatments in blocks 1–5. We replicated this design in three soil treatments: sterile, non-sterile, and preconditioned.
Each circle represents an experimental treatment. Letters represent individual plants. C = Col; V = Cvi; L = Ler; R = Rld.
We collected soil from fallow fields at the University of Massachusetts Suburban Experiment Station in Waltham, Massachusetts, USA (Lat = 42°23.1′N; Long = 71°12.9′W). We sieved the soil on site (to remove stones and macro-invertebrates), homogenized the soil, and stored it at 4°C until planting. All cone-tainers received a mixture of 10 g of field soil and 35 g of sterilized commercial potting soil. This mixture was established by Micallef et al.  as optimal for Arabidopsis growth. We sterilized the potting soil by autoclaving it in batches 1–2 cm deep. We autoclaved each batch twice, for 1 hr each time, with a 72 hour resting period in between (modified from Trevors 1996). We created a sterile soil treatment by sterilizing field soil with the same technique and adding it to the sterilized potting soil. To create the non-sterile treatment, we mixed non-sterile field soil with intact microbial communities from the field with sterilized potting soil. For this treatment we considered the non-sterilized field soil as a microbial inoculum from which the Arabidopsis plants could cultivate their unique soil communities. We created a “preconditioned” soil treatment by growing each of the four ecotypes in the non-sterile mix (non-sterile field soil with sterilized potting soil) for 6 weeks prior to the start of the experiment. We preconditioned the soil in large shallow greenhouse flats using a plant to soil ratio equal to that of pots in the final competition experiment. We kept the soils conditioned with different accessions separate from one another. We removed preconditioning plants from the soil and collected and mixed soils to homogenize soil within ecotype and removed all visible roots. We stored the soils for 24 hours in closed buckets before planting. When planting, we matched the preconditioned soil with the background accession.
We grew all plants with a 12-hour light regime in a plant-growth room, with 95 µmol PAR/m−2. After 7 weeks (6 weeks of focal plant growth) we harvested all the plants. We measured main inflorescence height, leaf number, and silique number for each focal plant. We then dried the aboveground tissue of all focal and background plants at 65°C and weighed it.
After harvesting the competition experiment we collected the soil from the preconditioned treatment for a preconditioning “follow-up” experiment. Keeping the soil preconditioned with the 4 different accessions separate, we split the soil into 3 soil treatments: fertilized, sterilized, and untreated. We gave the fertilized treatment 15 ml of half strength Scotts® Miracle Grow® (N, PO4, K, B, Cu, Fe, Mn, Mo, Zn, EDTA) solution, sterilized the sterile treatment as described for the competition experiment, and left the soil unchanged for the untreated treatment. In each pot we planted 3 seeds of each accession, with 6 replicates of each treatment. After 5 weeks we harvested all plants, dried the tissue at 65°C and weighed it.
Measurements of Competition Severity and Statistical Analyses
We calculated the absolute severity of competition (ASC; , ) for each treatment as:(1)Where Mi0 is the mass of accession i in the solo treatments, and Mij is the mass of accession i (focal) when grown with background competitors of accession j. ASC provides a measure of the effect of competition from the background plants on the performance of the focal plant. We determined ASC using blocks 1–5, which contained solo treatments.
To analyze the competition experiment data we used a series of ANCOVAs with soil (sterile, non-sterile, preconditioned), focal accession (Col, Cvi, Ler, Rld), and background accession (Col, Cvi, Ler, Rld) as fixed effects, and background plant mass as a covariate, with a separate ANCOVA for each response variable. Additionally we performed a MANCOVA with all response variables and report Pillai's Trace, Wilks' Lambda, Hotelling's Trace, and Roy's Largest Root test statistics. To compare individual means we used post-hoc Tukey's HSD tests. Finally, to detect treatment effects on ASC we used a 3-way ANOVA with soil, focal accession, and background accession as fixed factors. We log-transformed all mass, silique number, and height data for the competition, follow-up, and precondition experiments to meet assumptions of parametric tests. To analyze the preconditioned follow-up experiment we first calculated the response variable as average mass per plant of a given accession in each pot (statistical analyses obtained identical results when the response variable was total mass per pot). We then used an ANOVA with soil treatment (fertilized, sterilized, untreated), plant accession (Col, Cvi, Ler, Rld), and preconditioned soil type (Col, Cvi, Ler, Rld) as fixed effects. Again, to compare individual means we used a post-hoc Tukey's HSD test.
Focal plants in sterile soil grew larger than those in non-sterile soil, and plants in both of these treatments grew significantly larger than those in the preconditioned treatment (Table 1). All plants grown in preconditioned soil were extremely small and their inclusion in ANOVAs led to ecologically meaningless, and statistically unreliable, significant interactions (due to violations in assumptions for parametric tests). Therefore, we included only sterile and non-sterile soil treatments in further analyses. For all response variables (mass, leaf number, inflorescence height, and silique number), the main effects of soil, focal accession, and background accession were all significant (Table 2). Plants grown in sterile soil were always larger, had more leaves, greater height, and more siliques. Col and Ler accessions had the greatest mass, and Cvi the least. Consequently, focal plants with Cvi background competitors grew larger than those with the other three accessions as background plants (Fig. 2.). All focal accessions had more leaves when grown with Cvi background plants. Rld and Ler also showed increased inflorescence height and silique number with Cvi background plants. However, this is not surprising given that Col and Cvi bolted later and had shorter inflorescences and fewer siliques in all treatments. Relative to Col and Ler, Rld was also a weak competitor. Rld background plants had a similar effect to Cvi background plants on focal plant growth (Fig. 3, Table 2.). Col had the greatest total increase in leaves with sterilization, while Cvi had the greatest relative increase in leaf number (Fig. 4, Table 2). Plants grown alone (solo) in sterile soil were larger then solo plants in non-sterile soil (one-way ANOVA F2,59 = 158.049, P<0.001). Consistent with the univariate test results, all the MANCOVA test statistics were significant for soil, plant accession, background accession, soil×focal accession, and focal accession×background accession interaction (all test statistics P<0.01). Therefore not only did individual response variables significantly differ, but we also saw significant separation of the multivariate group centroids with our treatments.
Mean mass of sterile, and non-sterile, solo plants are given by lines surrounded by dark gray and light gray regions depicting ±SE. Means and SE for solo plants are shown for reference and are the same for all background accessions because they grew without background plants (N = 15).
Letters indicate significant differences between treatments using a Tukey-Kramer post-hoc paired comparison adjustment (N = 15).
Letters indicate significant differences between treatments using a Tukey-Kramer post-hoc paired comparison adjustment (N = 15).
Neither soil treatment nor focal accession affected ASC. However, background accession did affect ASC (3-way ANOVA for ASC background accession, F3,132 = 17.149, P<0.001). ASC values for Cvi were smaller than the other three accessions, and Rld had smaller ASC values than Col and Ler accessions (Fig. 5).
Due to plant mortality we used the average mass of each accession per pot rather than total mass in each pot. To justify this we performed a linear regression that showed no correlations between the number of plants per accession per pot and the average mass of plants (r2 = 0.003, P = 0.351). Plants grew largest in soil treated with fertilizer, but also showed increased size in sterilized soil relative to the untreated soil (3-way ANOVA for mass: soil treatment F2,220 = 84.248, P<0.001; Tukey's HSD, fertilized-sterilized, P<0.001; fertilized-untreated, P<0.001; sterilized - untreated, P<0.001). Soil accession also had a significant effect on plant size (3-way ANOVA for mass: soil accession, F3,220 = 4.224, P = 0.006). Plants grown in Cvi preconditioned soil grew larger than those grown in Col or Rld preconditioned soil (Fig. 6; Tukey's HSD: Cvi-Col, P = 0.028; Cvi-Rld, P = 0.008). Finally, Col plants were larger than the Cvi and Ler accessions, and Rld plants were larger than Cvi plants (3-way ANOVA for mass: plant accession, F3,220 = 7.669, P = 0.000; Tukey's HSD: Col-Cvi P = 0.000; Col-Ler P = 0.032; Cvi-Rld P = 0.050).
We saw no evidence to support our hypothesis that the soil communities of different Arabidopsis accessions differentially affect Arabidopsis growth. However, as we expected, focal plant growth did vary with different accessions of background competitors. The preconditioned treatment did provide exaggerated results, though not in the manner that we predicted. Rather than intensifying any soil mediated effects on focal plant growth, the preconditioned soil suppressed the growth of all of the plants in the cone-tainer. Although there was no accession-specific soil effect on plant growth, the soil community did negatively influence plant growth, with all accessions experiencing the same positive effect of sterilization.
Consistent with the literature on competition between Arabidopsis accessions ,  we saw morphologic and phenotypic differences among Arabidopsis accessions, and a significant effect of accession on competition and our plant response variables. Col and Ler focal plants were larger and had more leaves than the more delicate Cvi and Rld accessions (Figs. 3, 4). Focal plants with Col and Ler in the background had smaller mass, shorter inflorescences, fewer leaves and fewer siliques than plants with Cvi or Rld accession in the background (Figs. 3,4). This is unsurprising as the Col line was selected for vigor from a Landsberg (Lan) population in a greenhouse setting. Rld began bolting and flowering before the other three accessions, and Ler followed as the next to bolt and flower. As a result at harvest these two focal accessions had the tallest inflorescences and greatest number of siliques (Fig. 3). However, these morphological differences were probably affected by the timing of harvest. Had the plants each grown to senescence we might have seen a different pattern. We chose the harvest time because older leaves decompose rapidly as plants senesce, affecting the leaf count and mass of the plant.
We saw no evidence that Arabidopsis accessions exerted stronger intra- rather than inter-accession competition (Fig. 5). Rather, all accessions grew best when competing with Cvi, the smallest of the four accessions in the experiment. Although they used different accessions of Arabidopsis, Cahill et al.  also saw competitive strengths vary among accessions. Using multiple accessions of Arabidopsis, Bossdorf et al.  tested for interspecific competition between Arabidopsis and Senecio vulgaris and Anagallis arvensis. Similar to our results on intraspecific competition, Bossdorf et al.  saw differences among Arabidopsis accessions in their competitive effects on neighboring plants as well as in their responses to neighboring plants. In addition to the increasing evidence that Arabidopsis competitive ability varies with accession, Weltzin et al.  found that invasion of Arabidopsis thaliana communities by the congener Arabidopsis suecica was unaffected by the genetic diversity (number of different accessions) in the community. Weltzin et al.  planted seeds of Arabidopsis suecica into already established Arabidopsis thaliana communities composed of 1, 2, 4 and 8 accessions. The accession composition of each Arabidopsis thaliana community was generated by drawing accessions at random (without replacement) from a pool of 23. They found that density of the individuals in the Arabidopsis thaliana community had a strong effect on the size and reproductive potential of the invader. It is clear the accession of Arabidopsis is an important factor in determining individual's competitive success, and that the density of Arabidopsis populations is a driving factor behind their population dynamics.
Soil treatment also had an effect on focal plant growth. Plants grown in sterile soil were larger than those grown in non-sterile soil, while the plants grown in preconditioned soil were drastically smaller (Table 1). To minimize differences in nutrient availability between our sterile and non-sterile treatments we used sterile potting soil as the great majority of the substrate in both the sterile and non-sterile soil treatments. The only difference in the treatments was the treatment of the field soil that was mixed in. Therefore we assume that differences in soil treatments are primarily due to differences in the soil community.
The negative feedback we observed in our sterile treatment is in agreement with the review by Kulmatiski et al.  that showed most feedbacks to be negative in direction. Kulmatiski et al.  compared the range of plant-soil feedback effect sizes from their meta-analysis to those of meta-analyses of pathogenic fungi , leaf-litter addition , seed limitation, seed feeders, above ground herbivores, total herbivores, viruses, leaf chewers, root feeders , and soil warming . In each case the effect sizes for plant-soil feedbacks were similar to or larger than those in the other meta-analyses. However, they found plant-soil feedback effect sizes to be smaller than those of plant competitors, plant diversity , below ground herbivores, pathogens, and nematodes . While grasses have exhibited the most negative feedbacks  Arabidopsis as an herbaceous annual would also be expected to have more negative plant-soil feedbacks than trees and other perennial species. These findings are in keeping with the hypothesis that negative feedbacks increase the rate of successional replacement, with early colonizers experiencing the most negative feedback.
In the follow-up experiment, plants grew largest in the soil treated with fertilizer, but also grew larger in sterilized soil. Further, the soil accession (accession with which the soil was preconditioned for the original experiment) had an effect on plant size. Nutrient depletion probably contributed to the stunted growth of plants in the preconditioned soil. However, the preconditioned follow-up experiment also provided evidence of soil communities influencing plant growth, in both general and accession-specific ways.
The preconditioning soil treatment revealed that there were soil-mediated influences on plant growth in this experiment. The preconditioning follow-up experiment suggested that nutrient depletion and microbial interactions could have been responsible. Below-ground interactions have been highlighted by recent related work that showed genetic differences in Arabidopsis accessions influence growth and intra-specific competition via root exudates . Biedrzycki et al.  saw increased lateral root development when Arabidopsis accessions were exposed to “stranger” (other accessions') root exudates in comparison to exposure to “sibling” (same accession) exudates. While this type of below-ground interaction may have affected our competition results, the extreme results of the preconditioned treatment suggest that it is also likely that competition for nutrients between plants and microbes affected our results. In the past it was thought that plants only used inorganic N that was left by microbial N mineralization. However, it is now acknowledged that plants, including Arabidopsis , are also able to use organic N, that they compete directly with microbes for this resource  and that the ability of plants to compete with microbes is critical for plant N acquisition and subsequent growth . Bardgett et al.  showed that, in a temperate grassland, microorganisms were able to sequester the majority of both organic and inorganic N sources, thereby limiting plant productivity. Furthermore, stimulation of the microbial community by glucose addition increases microbial acquisition of N and causes a decrease in plant productivity . We assume that our preconditioned soil had a higher microbial density, and therefore the severe nutrient limitation faced in the plants in this treatment was made more severe by plant-microbe competition for N. Increased growth of plants after both fertilization and sterilization of this soil is further evidence of the effect of this competition. There are several reasons why we may have seen an effect of preconditioning accession on plant size in our follow-up experiment. It may be further evidence of microbial interactions influencing plant growth, indicating that the Cvi generated microbial community had the weakest effect on plant growth. However, it may also be an indirect effect of nutrient depletion by preconditioning plants; because the Cvi are the smallest plants it is possible that the original Cvi plants that preconditioned the soil used the least amount of nutrients, leaving more available for subsequent plantings to utilize. Additionally it is possible that the Cvi preconditioning plants encourage less microbial growth, which in turn would mean less competition for nutrients for the subsequent plantings.
This experiment found clear differences in competitive ability among different accessions of Arabidopsis, and found that Arabidopsis accessions were consistently negatively affected by their soil communities. We did not isolate the mechanism for these negative effects; nor can we distinguish between the effects of pathogenic microbes and plant-microbe competition for nutrients. We found no evidence that accession-specific rhizobacterial communities differentially influence plant growth and competition. It is possible that the unique rhizobacterial communities are a non-adaptive consequence of accession specific exudates. However, we performed this experiment using a single soil inoculate that was foreign to each accession. Therefore it remains possible that the soil communities of each accession may respond differently in “home” vs. “foreign” soil environments in a manner that has consequences for plant growth.
We would like to thank Lauren Mullin, Melinda Gammon, Noah Goldstein, Reino Carlson, Fayeann Crawford, and Vesna Gamez. We are also grateful for the constructive and thoughtful advice of the anonymous reviewers.
Conceived and designed the experiments: AGA JSD ACC RK. Performed the experiments: AGA. Analyzed the data: AGA JSD. Contributed reagents/materials/analysis tools: AGA ACC RK. Wrote the paper: AGA JSD.
- 1. Klironomos JN (2002) Feedback with soil biota contributes to plant rarity and invasiveness in communities. Nature 417: 67–70.
- 2. Bever JD (2003) Soil community feedback and the coexistence of competitors: conceptual frameworks and empirical tests. New Phytologist 157: 465–473.
- 3. Micallef SA, Shiaris MP, Colon-Carmona A (2009) Influence of Arabidopsis thaliana accessions on rhizobacterial communities and natural variation in root exudates. Journal of Experimental Botany 60: 1729–1742.
- 4. Schweitzer JA, Bailey JK, Fischer DG, Leroy CJ, Lonsdorf EV, et al. (2008) Plant-soil-microorganism interactions: Heritable relationship between plant genotype and associated soil microorganisms. Ecology 89: 773–781.
- 5. Lavelle P, Lattaud C, Trigo D, Barois I (1995) Mutualism and biodiversity in soils. Plant and Soil 170: 23–33.
- 6. Hooper DU, Bignell DE, Brown VK, Brussaard L, Dangerfield JM, et al. (2000) Interactions between aboveground and belowground biodiversity in terrestrial ecosystems: Patterns, mechanisms, and feedbacks. Bioscience 50: 1049–1061.
- 7. McArthur JV, Kovacic DA, Smith MH (1988) Genetic diversty in natural-populations of a soil bacterium across a landscape gradient. Proceedings of the National Academy of Sciences of the United States of America 85: 9621–9624.
- 8. Westover KM, Bever JD (2001) Mechanisms of plant species coexistence: Roles of rhizosphere bacteria and root fungal pathogens. Ecology 82: 3285–3294.
- 9. Niu HB, Liu WX, Wan FH, Liu B (2007) An invasive aster (Ageratina adenophora) invades and dominates forest understories in China: altered soil microbial communities facilitate the invader and inhibit natives. Plant and Soil 294: 73–85.
- 10. Jordan NR, Larson DL, Huerd SC (2008) Soil modification by invasive plants: effects on native and invasive species of mixed-grass prairies. Biological Invasions 10: 177–190.
- 11. Kulmatiski A, Beard KH, Stevens JR, Cobbold SM (2008) Plant-soil feedbacks: a meta-analytical review. Ecology Letters 11: 980–992.
- 12. Ehrenfeld JG, Ravit B, Elgersma K (2005) Feedback in the plant-soil system. Annual Review of Environment and Resources 30: 75–115.
- 13. Kardol P, Cornips NJ, van Kempen MML, Bakx-Schotman JMT, van der Putten WH (2007) Microbe-mediated plant-soil feedback causes historical contingency effects in plant community assembly. Ecological Monographs 77: 147–162.
- 14. Kardol P, Bezemer TM, van der Putten WH (2006) Temporal variation in plant-soil feedback controls succession. Ecology Letters 9: 1080–1088.
- 15. Vanderputten WH, Vandijk C, Peters BAM (1993) Plant-specific soil-borne diseases contribute to succession in foredune vegetation. Nature 362: 53–56.
- 16. Bever JD, Westover KM, Antonovics J (1997) Incorporating the soil community into plant population dynamics: the utility of the feedback approach. Journal of Ecology 85: 561–573.
- 17. Ehrenfeld JG (2003) Effects of exotic plant invasions on soil nutrient cycling processes. Ecosystems 6: 503–523.
- 18. Reinhart KO, Callaway RM (2006) Soil biota and invasive plants. New Phytologist 170: 445–457.
- 19. Callaway RM, Thelen GC, Rodriguez A, Holben WE (2004) Soil biota and exotic plant invasion. Nature 427: 731–733.
- 20. van der Heijden MGA, Bardgett RD, van Straalen NM (2008) The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems. Ecology Letters 11: 296–310.
- 21. Bardgett RD, Streeter TC, Bol R (2003) Soil microbes compete effectively with plants for organic-nitrogen inputs to temperate grasslands. Ecology 84: 1277–1287.
- 22. Bartelt-Ryser J, Joshi J, Schmid B, Brandl H, Balser T (2005) Soil feedbacks of plant diversity on soil microbial communities and subsequent plant growth. Perspectives in Plant Ecology Evolution and Systematics 7: 27–49.
- 23. Marschner P, Yang CH, Lieberei R, Crowley DE (2001) Soil and plant specific effects on bacterial community composition in the rhizosphere. Soil Biology & Biochemistry 33: 1437–1445.
- 24. Zhang ZB (2003) Mutualism or cooperation among competitors promotes coexistence and competitive ability. Ecological Modelling 164: 271–282.
- 25. Bressan R, Hans B, Jian-Kang Z (2009) Abiotic stress tolerance: from gene discovery in model organisms to crop improvement. Molecular Plant 1: 1–2.
- 26. Biedrzycki ML, Jilany TA, Dudley SA, Bais HP (2010) Root exudates mediate kin recognition in plants. Communicative & Integrative Biology 3: 28–35.
- 27. James FC, Steven WK, Danny JG (2005) Differential genetic influences on competitive effect and response in Arabidopsis thaliana. Journal of Ecology 93: 958–967.
- 28. Bossdorf O, Shuja Z, Banta JA (2009) Genotype and maternal environment affect belowground interactions between Arabidopsis thaliana and its competitors. Oikos 118: 1541–1551.
- 29. Snaydon RW (1991) Replacement or additive designs for competition studies. Journal of Applied Ecology 28: 930–946.
- 30. Welden CW, Slauson WL (1986) The intensity of competition versus its importance: an overlooked distinction and some implications. Quarterly Review of Biology 61: 23–44.
- 31. Weltzin JF, Muth NZ, Von Holle B, Cole PG (2003) Genetic diversity and invasibility: a test using a model system with a novel experimental design. Oikos 103: 505–518.
- 32. Levine JM, Adler PB, Yelenik SG (2004) A meta-analysis of biotic resistance to exotic plant invasions. Ecology Letters 7: 975–989.
- 33. Xiong S, Nilsson CJ (1999) The effects of plant litter on vegetation: a meta-analysis. Ecology 87: 984–994.
- 34. Morris WF, Hufbauer RA, Agrawal AA, Bever JD, Borowicz VA, et al. (2007) Direct and interactive effects of enemies and mutualists on plant performance: a meta-analysis. Ecology 88: 1021–1029.
- 35. Rustad LE, Campbell JL, Marion GM, Norby RJ, Mitchell MJ, et al. (2001) A meta-analysis of the response of soil respiration, net nitrogen mineralization, and aboveground plant growth to experimental ecosystem warming. Oecologia 126: 543–562.
- 36. Paungfoo-Lonhienne C, Lonhienne TGA, Rentsch D, Robinson N, Christie M, et al. (2008) Plants can use protein as a nitrogen source without assistance from other organisms. Proceedings of the national academy of sciences of the Untied States of America 105: 4524–4529.
- 37. Schimel JP, Bennett J (2004) Nitrogen mineralization: challenges of a changing paradigm. Ecology 85: 591–602.
- 38. Kaye JP, Hart SC (1997) Competition for nitrogen between plants and soil microorganisms. Trends in Ecology and Evolution 12: 139–143.
- 39. Dunn R, Mikola J, Bol R, Bardgett RD (2006) Influence of microbial activity on plant-microbial competition for organic and inorganic nitrogen. Plant Soil 289: 321–334.