A changing global climate can threaten the diversity of species and ecosystems. We explore the consequences of catastrophic disturbances in determining the evolutionary and demographic histories of secluded marble trout populations in Slovenian streams subjected to weather extremes, in particular recurrent flash floods and debris flows causing massive mortalities. Using microsatellite data, a pattern of extreme genetic differentiation was found among populations (global FST of 0.716), which exceeds the highest values reported in freshwater fish. All locations showed low levels of genetic diversity as evidenced by low heterozygosities and a mean of only 2 alleles per locus, with few or no rare alleles. Many loci showed a discontinuous allele distribution, with missing alleles across the allele size range, suggestive of a population contraction. Accordingly, bottleneck episodes were inferred for all samples with a reduction in population size of 3–4 orders of magnitude. The reduced level of genetic diversity observed in all populations implies a strong impact of genetic drift, and suggests that along with limited gene flow, genetic differentiation might have been exacerbated by recurrent mortalities likely caused by flash flood and debris flows. Due to its low evolutionary potential the species might fail to cope with an intensification and altered frequency of flash flood events predicted to occur with climate change.
Citation: Pujolar JM, Vincenzi S, Zane L, Jesensek D, De Leo GA, Crivelli AJ (2011) The Effect of Recurrent Floods on Genetic Composition of Marble Trout Populations. PLoS ONE6(9): e23822. https://doi.org/10.1371/journal.pone.0023822
Editor: Sergios-Orestis Kolokotronis, Barnard College, Columbia University, United States of America
Received: April 1, 2011; Accepted: July 26, 2011; Published: September 8, 2011
Copyright: © 2011 Pujolar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work has been funded by a contract by Fondation Tour du Valat to LZ. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Climate change poses a serious threat to species persistence. Many species will experience selection in new directions and at new intensities, and the degree to which species respond adaptively to dynamic and uncertain futures will determine their survival over the coming decades and millenia . Intensification of weather extremes and associated catastrophic disturbances is emerging as one of the most important aspects of climate change and research is advancing from studying the impacts of changes in mean climate values (trend effects) to those produced by changes in the magnitude or frequency of extreme events (event effects) . Catastrophes are characterized by their statistical extremeness combined with a short duration relative to the life cycle of the organisms affected; they can disrupt ecosystem, community or population structure and change resources, substrate availability, or the physical environment –. Evidence suggests that the frequency and intensity of extreme weather events (i.e. floods, droughts) is increasing in many regions in response to global climate change –. Despite the urgent need to advance research on extreme events and catastrophic disturbances, their evolutionary consequences have largely been unexplored .
Freshwater salmonids are commonly subject to substantial environmental variability in the form of changes in stream flow at different time scales –. Extreme events such as floods, droughts or landslides play an important role in the regulation of population dynamics in salmonids, to the extent that in high-density streams, fish demography and persistence might be largely shaped by extreme flow events –. The direct and short-term effects of floods are largely a result of high-water velocities and sediment movement that cause the displacement and death of fish. The impact of floods is expected to be higher in the coming years. According to IPCC predictions, an increase of rainfall is expected in the next 50 years along with an intensification and altered frequency of catastrophic disturbances . Recent advances in the statistical theory of extreme events suggest that large flood events will be more frequent, with return times markedly shorter than expected even in the absence of climate change .
Our model system is the stream-dwelling salmonid marble trout Salmo marmoratus, an endemism of the Adriatic Sea and its tributaries, currently restricted to Northern Italy, former Yugoslavia (Slovenia, Croatia, Bosnia-Herzegovina) and Albania . Marble trout is considered to be one of the most endangered species in the Adriatic basin . For decades, massive restocking practices have been conducted throughout its natural range by means of introduction of exotic brown trout. Hybridization between marble and brown trout has been so extensive that hybrids now dominate most rivers . Molecular data confirm a high level of introgression in the Po river in Northern Italy , , the Soca river system in the Italian/Slovenian border , , , and recently, the Adige river system in South Tyrol , . However, all studies also reported pure populations of marble trout in headwaters of all river systems, persisting above natural barriers (i.e. waterfalls). While those barriers prevent the upstream migration of conspecifics and consequent hybridization, the secluded nature and the small size of the remnant marble trout populations makes them extremely vulnerable to stochastic factors, including environmental events such as floods, droughts or landslides.
A project for the conservation and rehabilitation of marble trout in Slovenia started in 1993 . Only seven remnant pure (non-introgressed with brown trout; ) populations of marble trout remain in the Adriatic basin of Slovenia, persisting in totally isolated headwaters without predators or fishing . Recurrent major floods and debris flows are the most important risk factor for the viability of marble trout populations in Slovenian streams , . The trigger of debris flows in the area is extreme precipitation, with records of intense flows going back to the 18th century, and with a presumable occurrence interval of approximately 50 years (i.e. in the 20th century major floods were recorded in 1926, 1954 and 1990; ). However, an intensification of the frequency of flash floods has been observed in the last decade, with four important flood events recorded in the 1999–2010 period (Table 1).
The aim of this study is to explore the impact of catastrophic weather events on the genetic composition of isolated marble trout populations from the Adriatic basin of Slovenia (Figure 1). As a result of intense precipitation and associated flash flood and debris flows, which causes mass mortalities but does not alter connectivity among isolated streams and basins, low levels of genetic diversity and high genetic substructuring at local geographic scale are expected. In this study, we were particularly interested in the comparison of pre- and post-flood samples, testing shifts in genetic composition and genetic diversity and estimating the possible existence of bottleneck/population declines.
A total of 18 out of 24 loci were polymorphic, showing from 2 to 8 alleles per locus (Table 2). All locations showed low levels of genetic diversity as evidenced by heterozygosities <0.25 and allelic richness <2 (Table 3). Many loci showed a discontinuous allele distribution, with missing alleles across the allele size range, and few or no rare alleles (Table S1). A total of 31 location-specific private alleles were found, representing 49% of the total number of alleles sampled. Across locations, Studenc showed the highest genetic diversity, with intermediate levels found in Zadlascica and the lowest genetic variability found in Lipovscek and Sevnica (Table 3). When comparing pre- and post-flood samples, a moderate drop in genetic diversity was observed in all four locations in terms of Ho and He and allele richness (AR), mostly due to the loss of rare alleles (Table 3; Table S1). However, statistic comparisons between pre- and post-flood values of genetic diversity were non-significant at all locations: Zadlascica (Ho: p = 0.714; He: p = 0.891; AR: p = 0.747), Lipovscek (Ho: p = 0.650; He: p = 0.650; AR: p = 0.396), Sevnica (Ho: p = 0.591; He: p = 0.554; AR: p = 0.551) and Studenc (Ho: p = 0.726; He: p = 0.451; AR: p = 0.999).
All loci were at HWE after Bonferroni correction. A neutrality test using LOSITAN suggested no loci to be subject to balancing selection or directional selection. No difference in values of genetic diversity was found between EST-derived microsatellites and genomic microsatellites, including Ho (EST-microsatellites: 0.126; genomic microsatellites: 0.195; p = 0.232), He (EST-microsatellites: 0.138; genomic microsatellites: 0.180; p = 0.464) and AR (EST-microsatellites: 2.87; genomic microsatellites: 2.78; p = 0.964).
A highly significant extreme overall genetic differentiation was found among samples (FST = 0.716; p<0.001). All pairwise FST and genetic distances between samples from different locations were high (FST = 0.649–0.779; DCE = 0.592–0.839) and statistically significant (p<0.001; Table 4). By contrast, comparison of pre- and post-flood samples from the same location showed low non-significant FST and genetic distances with the exception of Studenc (FST = 0.041, p = 0.020; DCE = 0.021, p = 0.035). Accordingly, comparison of allele frequencies between pre- and post-flood samples using an exact test showed no temporal differences at Lipovscek (p = 0.993), Sevnica (p = 0.450) and Zadlascica (p = 0.985), while significant differences were found at Studenc (p<0.001) caused by loci CA059136 and Str151.
Accordingly, an AMOVA analysis partitioned genetic differentiation significantly among locations (FCT = 0.713; p<0.001) but not among (pre- and post-flood) samples within locations (FSC = 0.011; p = 0.067) (Table 5). We conducted a Multidimensional Scaling analysis using DCE at all loci (Figure 2). When plotting the values of the first and second principal components, samples clustered according to location, the Zadlascica location (from a tributary of the Soca river) appeared very different from the rest of locations (from tributaries of the Idrijca river), but so did Studenc despite being 20 km apart from Sevnica. In this sense, when testing Isolation-by-Distance, no correlation was found between genetic and geographic (shortest waterway) distances (r = 0.715; F = 4.19; p = 0.110). Assignment analysis using STRUCTURE confirmed the extreme genetic differentiation among locations and suggested a scenario with K = 4 groups as the most likely (p<0.001), corresponding to the four sampled locations, Zadlascica, Lipovscek, Sevnica and Studenc.
Samples labeled as in Table 3. A = pre-flood samples; B = post-flood samples. Stress value = 0.062.
The MSVAR procedure for assessing past demographic history strongly supported a decline in all marble trout populations. All sampled points of log10(r) were substantially below zero in all eight samples, suggesting that the past population size was larger than the current population size (Figure 3). All sampled points of log10(θ) were negative, pointing to small current effective population sizes. Using a conservative mutation rate of μ = 10−4, N1 varied across locations between 3 and 41 individuals, with a maximum upper-bound CI of N1 of 396 individuals (Table 6). Using higher mutation rates (i.e. 5×10−4 and 10−3), N1 values <than 1 were obtained, while lower mutation rates (i.e. 5×10−5) yielded unrealistic N0 values. The time of the bottleneck varied between 31 and 268 generations across locations (or roughly 100–800 years assuming a generation time of 2.7–3 years), with a maximum upper-bound CI of 2300 generations (roughly 7000 years).
Bottleneck-detection tests (Table 7) using the sofware BOTTLENECK and M_P_VAL showed some indications of population contraction at all locations. Garza and Williamson  suggested that values of M lower than 0.7 would indicate evidence of a bottleneck, while values above 0.8 would denote no bottleneck history. In our data set, all Zadlascica and Studenc samples plus the pre-flood Lipovscek sample showed M values between 0.518–0.661. The two Sevnica samples plus the post-flood Lipovscek sample showed M values between the 0.7–0.8 limits.
Extreme differentiation among marble trout populations
Freshwater fish species show a greater average degree of genetic differentiation among locations than marine species, resulting from the isolation of fish populations among drainages . The marked zoogeography produced by the historical patterns of isolation among drainages is subjected to the continuous remodeling of the river drainage and to climatic fluctuations of which the glacial/interglacial periods of the Pleistocene played a crucial role . Species respond actively to fluctuations in their natural range, with hydrographic networks being used for colonization and for retreat throughout geological times. Higher levels of population subdivision have been found in many salmonid studies reflecting complex genetic structures often resulting from isolation among drainages. In brook charr Salvelinus fontinalis, a global FST of 0.37 was found among 26 populations from La Maurice National Park in Canada located 3–42 km apart . In cutthroat trout Oncorhynchus clarkii, Taylor et al.  found an overall FST of 0.32 and pairwise FST values up to 0.45 in populations from British Columbia, while the recent study of Pritchard et al.  reported a global FST of 0.41 for Rio Grande populations. In bull trout Salvelinus confluentus, Taylor and Costello  found an FST = 0.33 among 20 North-West America coastal locations, while recent papers reported an overall FST of 0.15 and pairwise FST values up to 0.31 in Alberta  and pairwise FST values up to 0.66 between lake samples in a 50 km area off Montana .
Collectively, the extreme level of genetic differentiation found among Slovenian marble trout populations (overall FST = 0.716; pairwise FST between drainages = 0.649–0.779; 49% private alleles sampled) exceeds the highest values reported in salmonid populations found above waterfalls and/or in small streams like the ones in this study. Such extremely high FST is indicative of complete genetic isolation that has persisted over time. This is concordant with the previous study of Fumagalli et al. , which reported a global FST of 0.66 in the same area using a smaller number of microsatellites. At present, the four locations in our study are completely isolated from each other and from the main river drainage by means of impassable natural barriers (waterfalls) that restrict dispersal abilities. Baloux and Lugon-Moulin  argued that even in the total absence of gene flow, extreme genetic differentiation of the magnitude found in our study is not expected due to the high mutation rate of microsatellites , with appearance of new variants counteracting within-population fixation of alleles. However, the low level of genetic diversity observed in all locations (low heterozygosities and low number of alleles) implies a strong impact of local genetic drift, and suggests that, together with absence of gene flow, genetic differentiation among locations may have been exacerbated by recurrent mortalities likely caused by flash flood and debris flows. The combined effects of genetic drift and inbreeding following demographic bottlenecks may have thus contributed to alter distinctly the genetic composition of each population, so that over time different populations ended up having different alleles at each locus as well as different allele combinations from multiple loci, resulting in the observed pattern of extreme genetic differentiation.
The MSVAR procedure clearly suggested a population contraction with a reduction in population size of 3–4 orders of magnitude to current effective densities of around 3–41 (CI: 1–396) individuals per location. Bottleneck signatures were observed in all samples. First, most polymorphic loci presented a biallelic pattern with few (or none) rare alleles. Second, a discontinuous allele range was found at many loci (Table S1). For instance, only alleles 104, 110, 118 and 124 were sampled across locations at locus Strutta58, which suggests that the non-observed alleles have been lost over time. By contrast, samples collected in tributaries of the river Po in Northern Italy showed a continuous allele distribution and a higher number of alleles at five microsatellite loci , suggestive of demographically stable populations with no signatures of bottlenecks. Loci Ssa85 and Str15, which were monomorphic in Slovenian locations (our study), showed 6–9 alleles in Italian locations. Loci Str85, Str543 and Str591, which presented three alleles each in our study, showed higher allelic richness in the Italian samples (7–17 alleles). Pujolar et al.  also reported a lower genetic differentiation among Italian pure marble trout locations (global FST of 0.235). The different pattern suggested for Italian and Slovenian populations of marble trout, with signatures of a bottleneck only observed in the latter, might be due to the particular geography and the extreme effect of meteorological events in the region of Slovenia studied. Rainfall data have been acquired since 1961 (ARSO, Environmental Agency of Slovenia). While the annual mean precipitation was 2,400 mm in the 1961–2004 period, showing little variation across years, monthly rainfall showed up to 500-fold variation. Morphological features of the streams and watersheds in the region (i.e. high slopes, narrow walls, stream bed fragmentation, limited flood plain) are largely responsible for the high water flow in the stream after heavy rainfalls, and might explain the massive mortalities caused by flood events.
In contrast with the extreme differentiation found at microsatellite loci, one single mitochondrial DNA control region haplotype (MA1) has been reported for marble trout in Slovenia , . This suggests that despite the current complete geographic isolation of marble trout populations in Slovenian streams, those populations were interconnected in a relatively recent past prior to the fragmentation of habitats. On the basis of microsatellite data, Fumagalli et al.  suggested that the pure populations within the Idrijca drainage belonged to an independent river system. Nevertheless, our analysis on a larger number of microsatellite loci does not support the division by river basins, as the two locations from the Idrijca basin in our sampling (Sevnica and Studenc) did not cluster together in the Multidimensional Scaling analysis, and appeared clearly differentiated despite being merely 20 km apart within the same river basin.
Genetic consequences of serial bottlenecks
The analysis of pre- and post-flood samples in our study allowed us to explore the genetic consequences of population bottlenecks. The effects of bottlenecks are directly related to the increase of stochastic events associated with small population sizes, leading in most cases to a loss in genetic variability . Recently, Bouzat  suggested that the potential genetic outcomes of demographic bottlenecks can only be assessed when considering replicated bottlenecked populations. In our study, we explored four separate locations that experienced episodes of massive mortalities with consequent reductions in population size ranging from 56 to 77%. Collectively, and taking into the consideration the magnitude of the demographic decline, only a limited genetic effect was observed. A moderate drop in genetic diversity was found in all locations, both in terms of heterozygosities and allelic richness, but all comparisons were statistically not significant. However, a total of 9 alleles were lost locally, in all the cases representing rare alleles with frequencies <0.05 prior to the flood that were no longer sampled after the flood events. Moreover, bottlenecks resulted in alteration of allele frequencies, particularly secondary alleles, which in some cases experienced a drop of 20–30% in frequency. This was notably observed at Studenc, where genetic composition was significantly different in pre- and post-flood samples, but not at the other populations.
The moderate reduction of allelic diversity and heterozygosity might be attributable to the particular demographic history of those populations. In his recent review, Bouzat  emphasized the potential role of population history in determining the outcome of demographic bottlenecks. Specifically, one can expect that populations experiencing recurrent bottlenecks might have had their genetic pool already eroded over time, which would decrease the effectiveness of both purifying selection and random allele elimination. This holds particularly true for the Slovenian marble trout populations in our study, which have been repeatedly impacted by severe flood events, with a presumable occurrence interval of 50–100 years . Bayesian demographic analysis using the MSVAR procedure is concordant with a scenario of serial bottleneck episodes that might have been occurring for 150–1340 years.
On the question of how do the Slovenian population still maintain some polymorphism despite experiencing flood events for hundreds of years, one possible explanation is that our sampling is not representative of the entire population, and other compartments of the population might keep some genetic variation. One hypothesis could be that young fish (age 0+ and 1+) might be less sensible to flows because usually they are not found in the main stream but in more protected small tributaries, repopulating the stream even if all adults have been removed. However, field observations suggest that eggs and young fish are more vulnerable to floods than adults and that there is no recruitment at all when floods occur after reproduction (Crivelli, unpublished information). Alternatively, an upstream part of the population could be preserved. This hypothesis is supported by tagging data (Crivelli, unpublished information) that show non-tagged individuals in post-flood samples, whereas all pre-flood samples had been tagged, which suggests that the newly-arrived non-tagged individuals came from a compartment of the population located upstream, flushed by the flood.
Coping with environmental change
Our findings strongly suggest that the evolutionary and demographic history of marble trout populations living in secluded Slovenian streams has been shaped by massive mortalities caused by disturbance factors such as flash flood and debris flows. Overall, genetic analysis revealed an extremely high genetic differentiation among remnant pure populations, together with much low levels of genetic diversity in all populations. Effective population sizes estimated using the MSVAR procedure ranged from 3 to 41 (CI:1–396) individuals per population. The low genetic variability found does not seem to affect the viability of the populations so far, as they have persisted up to the present despite recurrent and unpredictable disturbances. To mitigate their ecological impact, fish populations might exhibit several adaptations that result from trade-off among growth, reproduction and survival. According to life-history theory, recurrent floods can have important evolutionary consequences by selecting for life-histories that are synchronized to either avoid or exploit the direct and indirect effects of extreme flows . However, due to the predicted intensification of extreme weather leading to increased floods, heatwaves, droughts and rainfall in the next 50 years , , marble trout may not have the sufficient genetic potential for the evolution of life-histories able to mitigate their impact. Recent studies by the European Commission's Joint Research Centre (JCR) have confirmed rising temperatures (around 1.5°C in the last 35 years) and higher levels of precipitation in Slovenia, with a projected increase in the occurrence of extreme rainfall events and flash floods. For 2020, Lehner et al.  predict major flood events for the Adriatic basin of Slovenia to become more frequent and intense. This might jeopardize the survival of marble trout as the occurrence of consecutive floods in a very short period of time could wipe out the population. This nearly occurred in the Lipovscek stream: a good recovery was observed following the 2004 flood, but in September 2007 a new important debris flood occurred with a mortality of 92.4% that left only 38 fish in the whole population. After a new major flood in 2009 followed by a moderate flood in 2010, the population has been decimated to only 10 individuals, so that at present, the recovery of Lipovscek remains uncertain (Crivelli, unpublished information). Medium flows (10 to 20-year recurrence interval) have also become more frequent with 3–5 moderate flow events observed in the last 10 years in the four populations in our study, and additionally, since 2009 spring floods have been observed for the first time in the region.
Collectively, while the Slovenian marble trout populations have coped successfully with recurrent yet unpredictable disturbance events over time, the low genetic variation found in the species makes it difficult to assess its evolutionary potential since a genetically depauperate population might fail to adapt to future environmental change. Moreover, all populations are closed, and there is no potential for spontaneous colonization of new habitats or re-colonization after local extinction. It has been proposed that an effective population size of 500 individuals is large enough to maintain genetic diversity for key history traits . The estimation in our study of effective population sizes of 3–41 individuals is one-two orders of magnitude lower than the threshold of 500 individuals, plus the upper-bound CI of population size does not overlap with the threshold value. This suggests that the Slovenian marble trout populations are beyond the critical population size for genetically secure populations. The future development of an integrated genetic/ecologic model that explores different demographic scenarios with varying degree of intensity and frequency of flood events might help disentangling the role of catastrophic disturbances in determining the genetic structure and genetic diversity of marble trout.
Materials and Methods
All animal work was approved by the Ministry of Agriculture, Forestry and Food of Republic of Slovenia and the Fisheries Research Institute of Slovenia. Original title of the Plan: RIBISKO - GOJITVENI NACRT za TOLMINSKI RIBISKI OKOLIS, razen Soce s pritoki od izvira do mosta v Cezsoco in Krnskega jezera, za obdobje 2006–2010. Sampling was supervised by the Tolmin Angling Association (Slovenia).
A total of 223 pure marble trout Salmo marmoratus individuals were caught using electrofishing at four tributaries of the Soca (1.Zadlascica), Baca (2.Lipovscek) and Idrijca (3.Sevnica and 4.Studenc) rivers in Slovenia (Figure 1). During field work fin clips were collected from anaesthetised individuals that were tagged and immediately released back into the streams. At Lipovscek, Sevnica and Studenc, samples were obtained in September 2004 prior to a flooding event that caused a mortality of 56.4%, 77.6%, and 67.9% in fish ≥ age-1, respectively. Post-flood samples were obtained at all three locations in September 2005. At Zadlascica, samples were obtained in September 2007 prior to a flooding event that caused a 74.9% drop in population size. Post-flood samples from Zadlascica were obtained in September 2008. Number of individuals in post-flood samples were constrained to the high mortalities and consequent low number of survivors, with only 15 individuals left in the case of Zadlascica after the flash flood event.
Minute sections of tissue from ethanol-preserved finclips were digested in a lysis buffer containing 100 µl TE Buffer, 7 µl 1 M DTT (dithiothreitol) solution pH 5.2 (diluted in 0.08 M NaAC) and 2 µl proteinase K solution (20 mg/ml) for at least 8 hours at 56°C. After incubation at 96°C for 10 min, samples were centrifuged at 13,000 rpm for 11 min, and the supernatant was stored at −20°C.
All samples were scored for a total of 24 microsatellite loci (Table 2). 15 loci were derived from Expressed Sequence Tags (ESTs) previously described by Vasemagi et al.  and Siemon et al.  in Atlantic salmon Salmo salar. We selected those loci with the highest genetic variation and a positive cross-species amplification in brown trout Salmo trutta. Additionally, 9 genomic microsatellite loci isolated and characterized from other salmonids, seven from brown trout (Str73, Str151: ; Str85, Str543, Str491: ; T3-13: ; Strutta58: ), one from Atlantic salmon (Ssa85: ) and one from marble trout (BFRO001: ) were amplified and scored. Microsatellites were grouped in two separate multiplexes in order to reduce polymerase chain reaction (PCR) and genotyping costs (Table 2).
PCR products were obtained in a GeneAmp PCR System 2700 Thermocycler (Applied Biosystems) using the QIAGEN Multiplex PCR Kit. PCR reactions consisted of 2 µl template DNA, 5 µl QIAGEN Multiplex PCR Master Mix, 0.2 µl 10 µM forward and reverse primers, and water up to 10 µl. PCR conditions were as follows: 3 min at 95°C, 35 cycles of 30 sec at 94°C, 90 sec at 57°C and 1 min at 72°C, and final elongation for 5 min at 60°C. PCR products were visualized in 1.8% agarose gels and screened for microsatellite polymorphism using an ABI 3130 AVANT automatic capillary sequencer (Applied Biosystems). Alleles were sized according to a Liz500 (50–500 bp) marker.
Within-sample genetic diversity statistics were assessed by observed (Ho) and expected (He) heterozygosities per locus using GENETIX version 4.05  and allelic richness (AR) using FSTAT . Differences in genetic diversity among samples were tested by one-way ANOVA using STATISTICA version 6.0 (StatSoft Inc.). Deviations from Hardy-Weinberg Equilibrium (HWE), linkage disequilibrium and differences in allele and genotype frequencies among samples were tested using GENEPOP version 3.4 .
Neutrality of the markers was tested using the software LOSITAN , which implements a FST outlier detection approach. This method evaluates the relationship between FST and expected heterozygosity (He), describing the expected distribution of Wright's inbreeding coefficient FST vs. He under an island model of migration with neutral markers. This distribution is used to identify outlier loci that show excessive high or low FST compared to neutral expectations. Such outlier loci are candidates for being subject to selection. We used the 0.99 criterion in order to minimize false positives as suggested by the authors.
Population structure was explored using non-hierarchical and hierarchical F-Statistics  calculated using ARLEQUIN . First, overall and pairwise FST values were calculated. Genetic differentiation was also partitioned among locations (4 locations: Zadlascica, Lipovscek, Sevnica and Studenc) and among samples within locations (pre- and post-flood samples). Significance tests were assessed with 10,000 permutation tests. In all cases, significance levels were corrected for multiple comparisons using Bonferroni . Pairwise multilocus comparisons between samples were calculated by Cavalli-Sforza and Edwards  chord distance (DCE) and graphically represented by Multidimensional Scaling (MDS) analysis using STATISTICA version 7.0 (StatSoft). Isolation-by-Distance (IBD) was tested using a Mantel test implemented in GENETIX, by correlating linearized genetic distance (FST/(1−FST)) vs. geographic distance (shortest waterway distance measured along the streams between pairs of samples).
A model-based clustering algorithm as implemented in the software STRUCTURE  was used in order to infer the most likely number of populations in the data. The software organizes individuals into a predefined number of clusters (K) with a given likelihood, which may represent putative groups. The analysis was performed with 1<K<8 to account for population substructuring within species, using the admixture model and without a population prior. The most likely K was determined using the criterion of Evanno et al.  and then used to assign each individual. A burn-in length of 105 iterations followed by 106 additional Markov Chain Monte Carlo (MCMC) iterations were performed. A minimum of 5 runs were performed for each K to check repeatability of results.
Historical demographic changes were inferred using the Bayesian coalescent-based approach implemented in MSVAR . Using a strict stepwise mutation model, the procedure provides distributions of the exponential growth rate r = N0/N1 (where N0 is the present effective population size, N1 is the effective population size at the time of population expansion or decline), the time since the population started to expand or decline tf = ta/N0 (where ta is the number of generations since the beginning of the expansion/decline) and the genetic parameter θ = 2N0μ. A mutation rate of μ = 10−4 was used , . Rectangular priors were chosen for all parameters, with limits of (−9, +5) for log10(r), log10(tf) and log10(θ) and starting values of 1 for θ at each locus, r and tf. The analysis was performed for an exponential model of population change. We used 50,000 thinned updates and a thinning interval of 50,000 steps, with an initial 10% discarded as burn-in. Convergence was assessed using Tracer v1.4 .
Finally, we tested for a recent genetic bottleneck episode with two different approaches. First, we used the software BOTTLENECK , based on the principle that after a recent reduction of effective population size, number of alleles (k) decreases faster than heterozygosity (He) at polymorphic loci. Thus, in a recently bottlenecked population, the observed gene diversity is higher than the expected equilibrium gene diversity (Heq) which is computed from the observed number of alleles (k), under the assumption of a constant-size (equilibrium) population . We used the Multiple-step Stepwise (TPM) model , which consists of mostly one-step mutations but a small percentage of multi-step changes, and is the recommended model for microsatellite data sets rather than the Infinite Alleles (IAM) or Single-step Stepwise (SMM) models . The proportion of singlestep mutation events was set to 90% (variance = 12%). Observed and expected heterozygosities were compared using a Wilcoxon sign-rank test as suggested by Piry et al. . Second, we calculated M, the mean ratio between number of alleles (k) and range in allele size (r), assuming that during a bottleneck episode k decreases faster than r (M_P_Val; ). Hence, the value of M decreases when a population is reduced in size. Average M was calculated across loci and compared with the critical value Mcrit estimated after 10,000 simulations and assuming the population to be at equilibrium. In all simulations, three different values of θ were used (5, 10 and 20). A range of mutation models were examined and conservative values were used for ps (frequency of one-step mutations) and Δg (average size of non one-step mutations), ps = 0.90 and Δg = 3.5, respectively.
Conceived and designed the experiments: JMP SV DJ AC. Performed the experiments: JMP. Analyzed the data: JMP LZ. Contributed reagents/materials/analysis tools: LZ AC. Wrote the paper: JMP SV LZ GDL AC.
- 1. Carroll SP (2008) Facing change: forms and foundations of contemporary adaptation to biotic invasions. Mol Ecol 17: 361–372.SP Carroll2008Facing change: forms and foundations of contemporary adaptation to biotic invasions.Mol Ecol17361372
- 2. Jentsch A, Kryeling J, Beierkuhlein C (2007) A new generation of climate-change experiments: events, not trends. Front Ecol Environ 5: 365–374.A. JentschJ. KryelingC. Beierkuhlein2007A new generation of climate-change experiments: events, not trends.Front Ecol Environ5365374
- 3. Picket STA, White PS (1985) The ecology of natural disturbances and patch. Orlando: Academic Press. STA PicketPS White1985The ecology of natural disturbances and patchOrlandoAcademic Press
- 4. Wagner A (2003) Risk management in biological evolution. J Theor Biol 225: 45–57.A. Wagner2003Risk management in biological evolution.J Theor Biol2254557
- 5. IPCC (2007) Climate Change 2007: The Physical Science Basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge: Cambridge University Press. IPCC2007Climate Change 2007: The Physical Science Basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate ChangeCambridgeCambridge University Press
- 6. Min SK, Zhang X, Zwiers FW, Hegerl GC (2011) Human contribution to more-intense precipitation extremes. Nature Lett 470: 378–381.SK MinX. ZhangFW ZwiersGC Hegerl2011Human contribution to more-intense precipitation extremes.Nature Lett470378381
- 7. Pall P, Aina T, Stone DA, Stott PA, Nozawa T, et al. (2011) Anthropogenic greenhouse gas contribution to flood risk in England and Wales in autumn 2000. Nature Lett 470: 382–386.P. PallT. AinaDA StonePA StottT. Nozawa2011Anthropogenic greenhouse gas contribution to flood risk in England and Wales in autumn 2000.Nature Lett470382386
- 8. Turner WR, Bradley BA, Estes LD, Hole DG, Oppenheimer M, et al. (2010) Climate change: helping nature survive the human response. Conserv Lett 3: 304–312.WR TurnerBA BradleyLD EstesDG HoleM. Oppenheimer2010Climate change: helping nature survive the human response.Conserv Lett3304312
- 9. Grossman GD, Ratajczak RE, Crawford M, Freeman MC (1998) Assemblage organization in stream fishes: effects of environmental variation and interspecific interactions. Ecol Monogr 68: 395–420.GD GrossmanRE RatajczakM. CrawfordMC Freeman1998Assemblage organization in stream fishes: effects of environmental variation and interspecific interactions.Ecol Monogr68395420
- 10. Lake PS (2000) Disturbance, patchiness and diversity in streams. J N Am Benthol Soc 49: 210–217.PS Lake2000Disturbance, patchiness and diversity in streams.J N Am Benthol Soc49210217
- 11. Sato T (2006) Dramatic decline in population abundance of Salvelinus leucomaenis after a severe flood and debris flow in a high gradient stream. J Fish Biol 69: 1849–1854.T. Sato2006Dramatic decline in population abundance of Salvelinus leucomaenis after a severe flood and debris flow in a high gradient stream.J Fish Biol6918491854
- 12. Elliott JM, Hurley MA, Elliot JA (1997) Variable effects of droughts on the density of a sea trout Salmo trutta populations over 30 years. J Appl Ecol 34: 1229–1238.JM ElliottMA HurleyJA Elliot1997Variable effects of droughts on the density of a sea trout Salmo trutta populations over 30 years.J Appl Ecol3412291238
- 13. Jensen AJ, Johnsen BO (1999) The functional relationship between peak spring floods and survival and growth in juvenile Atlantic salmon (Salmo salar) and brown trout (Salmo trutta). Funct Ecol 13: 778–785.AJ JensenBO Johnsen1999The functional relationship between peak spring floods and survival and growth in juvenile Atlantic salmon (Salmo salar) and brown trout (Salmo trutta).Funct Ecol13778785
- 14. Vincenzi S, Crivelli AJ, Jesensek D, De Leo GA (2008) Total population density during the first year of life as a major determinant of lifetime body-length trajectory in marble trout. Ecol Freshw Fish 17: 515–519.S. VincenziAJ CrivelliD. JesensekGA De Leo2008Total population density during the first year of life as a major determinant of lifetime body-length trajectory in marble trout.Ecol Freshw Fish17515519
- 15. Vincenzi S, Crivelli AJ, Jesensek D, De Leo GA (2008) The role of density-dependent individual growth in the presistence of freshwater salmonid populations. Oecologia 156: 523–534.S. VincenziAJ CrivelliD. JesensekGA De Leo2008The role of density-dependent individual growth in the presistence of freshwater salmonid populations.Oecologia156523534
- 16. Katz RW, Parlange MB, Naveau P (2002) Statistics of extremes in hydrology. Adv Water Resour 25: 1287–1304.RW KatzMB ParlangeP. Naveau2002Statistics of extremes in hydrology.Adv Water Resour2512871304
- 17. Crivelli AJ, Poizat G, Berrebi P, Jesensek D, Rubin JF (2000) Conservation biology applied to fish: the example of a project for rehabilitating the marble trout in Slovenia. Cybium 24: 211–230.AJ CrivelliG. PoizatP. BerrebiD. JesensekJF Rubin2000Conservation biology applied to fish: the example of a project for rehabilitating the marble trout in Slovenia.Cybium24211230
- 18. Berrebi P, Povz M, Jesensek D, Cattaneo-Berrebi G, Crivelli AJ (2000) The genetic diversity of native, stocked and hybrid populations of marble trout in the Soca river, Slovenia. Heredity 85: 277–287.P. BerrebiM. PovzD. JesensekG. Cattaneo-BerrebiAJ Crivelli2000The genetic diversity of native, stocked and hybrid populations of marble trout in the Soca river, Slovenia.Heredity85277287
- 19. Meldgaard M, Crivelli AJ, Jesensek D, Poizat G, Rubin JF, et al. (2007) Hybridization between the endangered marble trout (Salmo marmoratus) and the brown trout (Salmo trutta): an in-stream experiment. Biol Conserv 136: 602–611.M. MeldgaardAJ CrivelliD. JesensekG. PoizatJF Rubin2007Hybridization between the endangered marble trout (Salmo marmoratus) and the brown trout (Salmo trutta): an in-stream experiment.Biol Conserv136602611
- 20. Giuffra E, Bernatchez L, Guyomard R (1994) Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy. Mol Ecol 3: 161–171.E. GiuffraL. BernatchezR. Guyomard1994Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy.Mol Ecol3161171
- 21. Giuffra E, Guyomard R, Forneris G (1996) Phylogenetic relationships and introgression patterns between incipient parapatric species of Italian brown trout Salmo trutta complex. Mol Ecol 5: 207–220.E. GiuffraR. GuyomardG. Forneris1996Phylogenetic relationships and introgression patterns between incipient parapatric species of Italian brown trout Salmo trutta complex.Mol Ecol5207220
- 22. Snoj A, Jug T, Milkic E, Susnik S, Pohar J, et al. (2000) Mitochondrial and microsatellite DNA analysis of marble trout in Slovenia. Quaderni ETP 29: 5–11.A. SnojT. JugE. MilkicS. SusnikJ. Pohar2000Mitochondrial and microsatellite DNA analysis of marble trout in Slovenia.Quaderni ETP29511
- 23. Fumagalli L, Snoj A, Jesensek D, Balloux F, Jug T, et al. (2002) Extreme genetic differentiation among the remnant populations of marble trout Salmo marmoratus in Slovenia. Mol Ecol 11: 2711–2716.L. FumagalliA. SnojD. JesensekF. BallouxT. Jug2002Extreme genetic differentiation among the remnant populations of marble trout Salmo marmoratus in Slovenia.Mol Ecol1127112716
- 24. Meraner A, Baric S, Pelster B, Dalla Via J (2007) Trout (Salmo trutta) mitochondrial DNA polymorphism in the centre of the marble trout distribution area. Hydrobiologia 579: 337–349.A. MeranerS. BaricB. PelsterJ. Dalla Via2007Trout (Salmo trutta) mitochondrial DNA polymorphism in the centre of the marble trout distribution area.Hydrobiologia579337349
- 25. Meraner A, Baric S, Pelster B, Dalla Via J (2010) Mitochondrial DNA data point to extensive but incomplete admixture in a marble and brown trout hybridization zone. Conserv Genet 11: 985–998.A. MeranerS. BaricB. PelsterJ. Dalla Via2010Mitochondrial DNA data point to extensive but incomplete admixture in a marble and brown trout hybridization zone.Conserv Genet11985998
- 26. Vincenzi S, Crivelli AJ, Jesensek D, Rubin JF, Poizat G, et al. (2008) Potential factors controlling the population viability of newly introduced endangered marble trout populations. Biol Conserv 141: 198–210.S. VincenziAJ CrivelliD. JesensekJF RubinG. Poizat2008Potential factors controlling the population viability of newly introduced endangered marble trout populations.Biol Conserv141198210
- 27. Zorn M, Natek K, Komac B (2006) Mass movements and flash-floods in Slovene Alps and surrounding mountains. Studia Geomorphological Carpatho-Balcanica 15: 127–145.M. ZornK. NatekB. Komac2006Mass movements and flash-floods in Slovene Alps and surrounding mountains.Studia Geomorphological Carpatho-Balcanica15127145
- 28. Garza JC, Williamson EG (2001) Detection of reduction in population size using data from microsatellite loci. Mol Ecol 10: 305–318.JC GarzaEG Williamson2001Detection of reduction in population size using data from microsatellite loci.Mol Ecol10305318
- 29. Ward RD, Woodwark M, Skibinsi DOF (1994) A comparison of genetic diversity levels in marine, freshwater and anadromous fish. J Fish Biol 44: 213–232.RD WardM. WoodwarkDOF Skibinsi1994A comparison of genetic diversity levels in marine, freshwater and anadromous fish.J Fish Biol44213232
- 30. Volckaert FAM, Hanfling B, Hellemans B, Carvalho GR (2002) Timing of the population dynamics of bullhead (Cottus gobio) during the Pleistocene. J Evol Biol 15: 930–944.FAM VolckaertB. HanflingB. HellemansGR Carvalho2002Timing of the population dynamics of bullhead (Cottus gobio) during the Pleistocene.J Evol Biol15930944
- 31. Angers A, Bernatchez L (1998) Combined use of SMM and non-SMM methods to infer structure an evolutionary history of closely-related brook charr (Salvelinus fontinalis) populations from microsatellites. Mol Biol Evol 15: 143–159.A. AngersL. Bernatchez1998Combined use of SMM and non-SMM methods to infer structure an evolutionary history of closely-related brook charr (Salvelinus fontinalis) populations from microsatellites.Mol Biol Evol15143159
- 32. Taylor EB, Stamford MD, Baxter JS (2003) Population subdivision in westlope cutthroat trout (Oncorhynchus clarkii lewisi) at the northern periphery of its range: evolutionary inferences and conservation implications. Mol Ecol 12: 2609–2622.EB TaylorMD StamfordJS Baxter2003Population subdivision in westlope cutthroat trout (Oncorhynchus clarkii lewisi) at the northern periphery of its range: evolutionary inferences and conservation implications.Mol Ecol1226092622
- 33. Pritchard VL, Metcalf JL, Jones K, Martin AP, Cowley DE (2009) Population structure and genetic management of rio Grande cutthroat trout (Oncorhynchus clarkii virginalis). Conserv Genet 10: 1209–1221.VL PritchardJL MetcalfK. JonesAP MartinDE Cowley2009Population structure and genetic management of rio Grande cutthroat trout (Oncorhynchus clarkii virginalis).Conserv Genet1012091221
- 34. Taylor EB, Costello AB (2006) Microsatellite DNA analysis of coastal populations of bull trout (Salvelinus confluentus) in British Columbia: zoogeographic implications and its application to recreational fishery management. Can J Fish Aquat Sci 63: 1157–1171.EB TaylorAB Costello2006Microsatellite DNA analysis of coastal populations of bull trout (Salvelinus confluentus) in British Columbia: zoogeographic implications and its application to recreational fishery management.Can J Fish Aquat Sci6311571171
- 35. Warnock WG, Rasmussen JB, Taylor EB (2010) Genetic clustering methods reveal bull trout (Salvelinus confluentus) fine-scale population structure as a spatially nested hierarchy. Conserv Genet 11: 1421–1433.WG WarnockJB RasmussenEB Taylor2010Genetic clustering methods reveal bull trout (Salvelinus confluentus) fine-scale population structure as a spatially nested hierarchy.Conserv Genet1114211433
- 36. Meeuwig MH, Guy CS, Kalinowski ST, Fredenberg WA (2010) Landscape influences on genetic differentiation among bull trout populations in a stream-lake network. Mol Ecol 19: 3620–3633.MH MeeuwigCS GuyST KalinowskiWA Fredenberg2010Landscape influences on genetic differentiation among bull trout populations in a stream-lake network.Mol Ecol1936203633
- 37. Balloux F, Lugon-Moulin N (2002) The estimation of population differentiation with microsatellite markers. Mol Ecol 11: 155–165.F. BallouxN. Lugon-Moulin2002The estimation of population differentiation with microsatellite markers.Mol Ecol11155165
- 38. Fraser DJ, Hansen MM, Ostergaard S, Tessier N, Legault M, et al. (2007) Comparative estimation of effective population sizes and temporal gene flow in two contracting population systems. Mol Ecol 16: 3866–3889.DJ FraserMM HansenS. OstergaardN. TessierM. Legault2007Comparative estimation of effective population sizes and temporal gene flow in two contracting population systems.Mol Ecol1638663889
- 39. Pujolar JM, Lucarda AN, Simonato M, Patarnello T (2011) Restricted gene flow at the micro- and macro-geographical scale in marble trout based on mtDNA and microsatellite polymorphism. Front Zool 8: 7.JM PujolarAN LucardaM. SimonatoT. Patarnello2011Restricted gene flow at the micro- and macro-geographical scale in marble trout based on mtDNA and microsatellite polymorphism.Front Zool87
- 40. Hedrick PW (2005) Genetics of populations. Sudbury: Jones & Barlett Publishers. PW Hedrick2005Genetics of populationsSudburyJones & Barlett Publishers
- 41. Bouzat JL (2010) Conservation genetics of population bottlenecks: the role of chance, selection, and history. Conserv Genet 11: 463–478.JL Bouzat2010Conservation genetics of population bottlenecks: the role of chance, selection, and history.Conserv Genet11463478
- 42. Lytle DA, Poff NL (2004) Geographic variation in patterns of nestedness among local stream fish assemblages in Virginia. Oecologia 140: 639–649.DA LytleNL Poff2004Geographic variation in patterns of nestedness among local stream fish assemblages in Virginia.Oecologia140639649
- 43. Walther GR, Post E, Convery P, Menzel A, Parmesan C, et al. (2002) Ecological responses to recent climate change. Nature 416: 389–395.GR WaltherE. PostP. ConveryA. MenzelC. Parmesan2002Ecological responses to recent climate change.Nature416389395
- 44. Lehner B, Döll P, Alcamo J, Henrichs T, Kaspar F (2006) Estimating the impact of global change on flood and drought risks in Europe: a continental integrated analysis. Climatic Change 75: 273–299.B. LehnerP. DöllJ. AlcamoT. HenrichsF. Kaspar2006Estimating the impact of global change on flood and drought risks in Europe: a continental integrated analysis.Climatic Change75273299
- 45. Frankham R (1995) Conservation Genetics. Annu Rev Genet 29: 305–327.R. Frankham1995Conservation Genetics.Annu Rev Genet29305327
- 46. Vasemagi A, Nilsson J, Primmer CR (2005) Seventy-five EST-linked Atlantic salmon (Salmo salar) microsatellite markers and their cross-amplification in five salmonid species. Mol Ecol Notes 5: 282–288.A. VasemagiJ. NilssonCR Primmer2005Seventy-five EST-linked Atlantic salmon (Salmo salar) microsatellite markers and their cross-amplification in five salmonid species.Mol Ecol Notes5282288
- 47. Siemon HSN, Chang A, Brown GD, Koop BF, Davidson WS (2005) Type I microsatellite markers from Atlantic salmon (Salmo salar) expressed sequence tags. Mol Ecol Notes 5: 762–766.HSN SiemonA. ChangGD BrownBF KoopWS Davidson2005Type I microsatellite markers from Atlantic salmon (Salmo salar) expressed sequence tags.Mol Ecol Notes5762766
- 48. Estoup A, Presa P, Krieg F, Valman F, Guyomard R (1993) (CT)n and (GT)n microsatellites: a new class of genetic markers for Salmo trutta (brown trout). Heredity 71: 488–496.A. EstoupP. PresaF. KriegF. ValmanR. Guyomard1993(CT)n and (GT)n microsatellites: a new class of genetic markers for Salmo trutta (brown trout).Heredity71488496
- 49. Presa P, Guyomard R (1996) Conservation of microsatellites in three species of salmonids. J Fish Biol 49: 1326–1329.P. PresaR. Guyomard1996Conservation of microsatellites in three species of salmonids.J Fish Biol4913261329
- 50. Estoup A, Gharbi K, SanCristobal M, Chevalet C, Haffray P, et al. (1998) Parentage assignment using microsatellite in turbot Scophthalmus maximus and rainbow trout Oncorhynchus mykiss hatchery populations. Can J Fish Aquat Sci 55: 715–725.A. EstoupK. GharbiM. SanCristobalC. ChevaletP. Haffray1998Parentage assignment using microsatellite in turbot Scophthalmus maximus and rainbow trout Oncorhynchus mykiss hatchery populations.Can J Fish Aquat Sci55715725
- 51. Poteaux C, Bonhomme F, Berrebi P (1999) Microsatellite polymorphism and genetic impact of restocking in Mediterranean brown trout (Salmo trutta). Heredity 82: 645–653.C. PoteauxF. BonhommeP. Berrebi1999Microsatellite polymorphism and genetic impact of restocking in Mediterranean brown trout (Salmo trutta).Heredity82645653
- 52. O'Reilly PT, Hamilton LC, McConnell SK, Wright JM (1996) Rapid analysis of genetic variation in Atlantic salmon (Salmo salar) by PCR multiplexing of dinucleotide and tetranucleotide microsatellites. Can J Fish Aquat Sci 53: 2292–2298.PT O'ReillyLC HamiltonSK McConnellJM Wright1996Rapid analysis of genetic variation in Atlantic salmon (Salmo salar) by PCR multiplexing of dinucleotide and tetranucleotide microsatellites.Can J Fish Aquat Sci5322922298
- 53. Snoj A, Pohar J, Dovc P (1997) The first microsatellite DNA marker for marble trout. J Anim Sci 75: 1983.A. SnojJ. PoharP. Dovc1997The first microsatellite DNA marker for marble trout.J Anim Sci751983
- 54. Belkhir K, Borsa P, Goudet J, Bonhomme F (2005) GENETIX 4.05: Logiciel sous Windows pour la genetique des populations. K. BelkhirP. BorsaJ. GoudetF. Bonhomme2005GENETIX 4.05: Logiciel sous Windows pour la genetique des populations.Laboratoire Genome and Population, CNRS-UPR/Universite de Montpellier II, Montpellier, France. Laboratoire Genome and Population, CNRS-UPR/Universite de Montpellier II, Montpellier, France.
- 55. Goudet J (2002) FSTAT version 126.96.36.199. J. Goudet2002FSTAT version 188.8.131.52.Available from: http://www2.unil.ch/popgen/softwares/fstat.htm. Available from: http://www2.unil.ch/popgen/softwares/fstat.htm.
- 56. Raymond M, Rousset F (1995) GENEPOP (version 1.2): a population genetics software for exact tests and ecumenicism. J Hered 86: 248–249.M. RaymondF. Rousset1995GENEPOP (version 1.2): a population genetics software for exact tests and ecumenicism.J Hered86248249
- 57. Antao T, Lopes A, Lopes RJ, Beja-Pereira A, Luikart G (2008) LOSITAN – a workbench to detect molecular adaptation based on FST-outlier method. BMC Bioinform 9: 323.T. AntaoA. LopesRJ LopesA. Beja-PereiraG. Luikart2008LOSITAN – a workbench to detect molecular adaptation based on FST-outlier method.BMC Bioinform9323
- 58. Weir BS, Cockerman CC (1984) Estimating F-Statistics for the analysis of population structure. Evolution 38: 1358–1370.BS WeirCC Cockerman1984Estimating F-Statistics for the analysis of population structure.Evolution3813581370
- 59. Schneider S, Roessli D, Excoffier L (2000) ARLEQUIN: a software for population genetics data analysis ver 3.0. S. SchneiderD. RoessliL. Excoffier2000ARLEQUIN: a software for population genetics data analysis ver 3.0.Genetics and Biometry Lab, Dept. of Anthropology, University of Geneva, Switzerland. Genetics and Biometry Lab, Dept. of Anthropology, University of Geneva, Switzerland.
- 60. Rice WR (1989) Analyzing tables and statistical tests. Evolution 43: 223–225.WR Rice1989Analyzing tables and statistical tests.Evolution43223225
- 61. Cavalli-Sforza LL, Edwards AWF (1967) Phylogenetic analysis models and estimation procedures. Evolution 32: 550–570.LL Cavalli-SforzaAWF Edwards1967Phylogenetic analysis models and estimation procedures.Evolution32550570
- 62. Pritchard JK, Stephens M, Donelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155: 945–959.JK PritchardM. StephensP. Donelly2000Inference of population structure using multilocus genotype data.Genetics155945959
- 63. Evanno G, Regnaut S, Houdet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14: 2611–2620.G. EvannoS. RegnautJ. Houdet2005Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study.Mol Ecol1426112620
- 64. Beaumont M (1999) Detecting population expansion and decline using microsatellites. Genetics 153: 2013–2029.M. Beaumont1999Detecting population expansion and decline using microsatellites.Genetics15320132029
- 65. Ellegreen H (2000) Microsatellite mutation in the germline: implications for evolutionary inference. Trends Genet 16: 551–558.H. Ellegreen2000Microsatellite mutation in the germline: implications for evolutionary inference.Trends Genet16551558
- 66. Van Oosterhout C, Joyce DA, Cummings SM, Blais J, Barson NJ, et al. (2006) Balancing selection, random genetic drift and genetic variation at the major histocompatibility complex in two wild populations of guppies (Poecilia reticulata). Evolution 60: 2562–2574.C. Van OosterhoutDA JoyceSM CummingsJ. BlaisNJ Barson2006Balancing selection, random genetic drift and genetic variation at the major histocompatibility complex in two wild populations of guppies (Poecilia reticulata).Evolution6025622574
- 67. Rambaut A, Drummond AJ (2007) Tracer version 1.4. A. RambautAJ Drummond2007Tracer version 1.4.Available from http://beast.bio.ed.ac.uk/Tracer. Available from http://beast.bio.ed.ac.uk/Tracer.
- 68. Piry SG, Luikart G, Cornuet JM (1999) BOTTLENECK: a computer program for detecting recent reductions in the effective population size using allele frequency data. J Hered 90: 502–503.SG PiryG. LuikartJM Cornuet1999BOTTLENECK: a computer program for detecting recent reductions in the effective population size using allele frequency data.J Hered90502503
- 69. Cornuet JM, Luikart G (1996) Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics 144: 2001–2014.JM CornuetG. Luikart1996Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data.Genetics14420012014
- 70. Di Rienzo A, Peterson AC, Garza JC, Valdes AM, Slatkin M, et al. (1994) Mutational processes of single-sequence repeat loci in human populations. P Natl Acad Sci USA 91: 3166–3170.A. Di RienzoAC PetersonJC GarzaAM ValdesM. Slatkin1994Mutational processes of single-sequence repeat loci in human populations.P Natl Acad Sci USA9131663170
- 71. Luikart G, Sherwin WB, Steele BM, Allendorf FW (1998) Usefulness of molecular markers for detecting population bottlenecks via monitoring genetic change. Mol Ecol 7: 963–974.G. LuikartWB SherwinBM SteeleFW Allendorf1998Usefulness of molecular markers for detecting population bottlenecks via monitoring genetic change.Mol Ecol7963974