Coral reefs are in severe decline. Infections by the human pathogen Serratia marcescens have contributed to precipitous losses in the common Caribbean elkhorn coral, Acropora palmata, culminating in its listing under the United States Endangered Species Act. During a 2003 outbreak of this coral disease, called acroporid serratiosis (APS), a unique strain of the pathogen, Serratia marcescens strain PDR60, was identified from diseased A. palmata, human wastewater, the non-host coral Siderastrea siderea and the corallivorous snail Coralliophila abbreviata. In order to examine humans as a source and other marine invertebrates as vectors and/or reservoirs of the APS pathogen, challenge experiments were conducted with A. palmata maintained in closed aquaria to determine infectivity of strain PDR60 from reef and wastewater sources. Strain PDR60 from wastewater and diseased A. palmata caused disease signs in elkhorn coral in as little as four and five days, respectively, demonstrating that wastewater is a definitive source of APS and identifying human strain PDR60 as a coral pathogen through fulfillment of Koch's postulates. A. palmata inoculated with strain PDR60 from C. abbreviata showed limited virulence, with one of three inoculated fragments developing APS signs within 13 days. Strain PDR60 from non-host coral S. siderea showed a delayed pathogenic effect, with disease signs developing within an average of 20 days. These results suggest that C. abbreviata and non-host corals may function as reservoirs or vectors of the APS pathogen. Our results provide the first example of a marine “reverse zoonosis” involving the transmission of a human pathogen (S. marcescens) to a marine invertebrate (A. palmata). These findings underscore the interaction between public health practices and environmental health indices such as coral reef survival.
Citation: Sutherland KP, Shaban S, Joyner JL, Porter JW, Lipp EK (2011) Human Pathogen Shown to Cause Disease in the Threatened Eklhorn Coral Acropora palmata. PLoS ONE6(8): e23468. https://doi.org/10.1371/journal.pone.0023468
Editor: Steve Vollmer, Northeastern University, United States of America
Received: February 28, 2011; Accepted: July 18, 2011; Published: August 17, 2011
Copyright: © 2011 Sutherland et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research was supported by Mote Marine Laboratory Protect Our Reefs grant POR-2008-23 (www.mote.org) to KPS and EKL, the Rollins College Student-Faculty Collaborative Research Program and Edward W. and Stella C. Van Houten Memorial Fund to SS and KPS, and by a Rollins College Critchfield Research Grant (www.rollins.edu) to KPS. Additional partial support was provided by National Science Foundation (NSF) grants EF-1015032 to KPS and EF-1015342 to JWP and EKL as part of the joint NSF-National Institutes of Health Ecology of Infectious Disease program (www.nsf.org) and by the US Environmental Protection Agency South Florida Water Quality Protection Program (www.epa.gov) to JWP. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Once the most common coral in the Caribbean, elkhorn coral Acropora palmata was listed for protection under the United States Endangered Species Act in 2006 , largely due to a disease unique to this coral species (Fig. 1). This disease, termed white pox or acroporid serratiosis (APS), is caused by the bacterium Serratia marcescens . S. marcescens is an opportunistic human pathogen, causing respiratory, wound and urinary tract infections, meningitis and pneumonia –. Human diseases caused by this bacterium have been associated with waterborne infections in tropical freshwaters , but are most often associated with nosocomial infections of neonates and immuno-compromised patients . During a 2003 outbreak, a unique strain of S. marcescens, strain PDR60, was identified from both diseased A. palmata and untreated human sewage, suggesting a causal link between wastewater and APS . In addition, strain PDR60 was isolated from potential vectors and reservoirs including the non-host corals Siderastrea siderea and Solenastrea bournoni and the coral predatory snail Coralliophila abbreviata .
A. Images of white pox affected A. palmata at Looe Key, from June 2008, to June 2009, to July 2009 (left to right), show colony growth and partial mortality. Scales bars are 3 cm on a side or 3 cm in diameter. (Photographs by JW Porter and MK Meyers) B. Average time (days) to development of disease signs on A. palmata inoculated with Serratia marcescens. Days to tissue loss was averaged for the three A. palmata fragments used in each treatment and each control. The original inoculum was recovered for all seven of the presented S. marcescens inocula, including five isolates of strain PDR60 collected from acroporid serratiosis (APS)-affected A. palmata (isolates 1, 2), non-host coral Siderastrea siderea (isolate 4), corallivorous snail Coralliophila abbreviata (isolate 5), and untreated wastewater (isolate 6). Two additional inocula included S. marcescens strain PDL100 (isolate 7), previously confirmed as an APS pathogen through fulfillment of Koch's Postulates (2) and S. marcescens strain WWI31 from untreated wastewater (isolate 8). The vehicle control exhibited tissue loss beginning at day 15. The E. coli-plus-vehicle control and the isolate 3-plus-vehicle treatment did not exhibit tissue loss and remained apparently healthy for the duration of the 26 day study. Virulent strains (isolates 1, 2, 5, 6, 8) caused tissue loss within 14 days of inoculation and attenuated strains (isolates 4, 7) caused tissue loss after day 14.
Koch's postulates, the preferred method for proving disease causation in humans and wildlife, require inoculation of a pure culture of a suspected pathogen isolated from a diseased host onto an apparently healthy host followed by development of disease signs and reisolation of the pathogen. Koch's postulates have proven difficult to fulfill for a number of coral diseases due in part to difficulty isolating and identifying potential pathogens and growing these organisms in pure culture. To date, the postulates have been satisfied for only five coral diseases, including APS with strain PDL100 isolated from a 1999 outbreak , . Unlike strain PDR60, isolated from host and non-host corals and snails, strain PDL100 has been identified only from diseased Acropora palmata . Here we hypothesize that strain PDR60 isolated from two distinct environments, one terrestrial (human wastewater) and one marine (APS-affected A. palmata, apparently healthy Siderastrea siderea and Coralliophila abbreviata) causes APS in A. palmata. To examine this hypothesis we conducted challenge experiments by inoculating eight isolates of Serratia marcescens representing three strains onto A. palmata fragments maintained in closed seawater aquaria. Our results confirm strain PDR60 as a coral pathogen through fulfillment of Koch's postulates. These results are also consistent with the hypothesis that non-host corals and predatory snails may function as interepizootic reservoirs or vectors of the APS pathogen. Furthermore, we show that S. marcescens isolated from human wastewater causes APS in as little as four days, unequivocally verifying humans as a source of a marine invertebrate disease.
Materials and Methods
Challenge Experiment Design
Experiments were conducted in June 2009 at the Mote Tropical Research Laboratory (MTRL), Summerland Key, FL. Eleven aquaria (20 L) were placed in a wet-table raceway with flowing 20 µm filtered groundwater and filled with 10 µm filtered seawater. A pump was placed in each aquarium, approximately 1 cm below the water surface, to maintain aeration. The raceway was covered with a plastic tarp and this shading plus water circulating around aquaria maintained aquaria temperatures at 28°C. Aquaria containing seawater acclimated for 24 hours before corals were added.
Small Acropora palmata fragments (25 cm2) were collected from 19 apparently healthy colonies at Western Sambo Reef, FL. Prior to fragment collection, the surface mucopolysaccharide layer (SML) was removed from each of the 19 coral colonies using sterile needle-less 20 cm3 syringes and placed in a cooler on ice. Coral fragments were placed in 1 gallon zip-lock plastic bags containing seawater and stored in a cooler without ice. SML and coral samples were transported to the MTRL. Within three hours of collection, SML samples were spread plated (100 µl) on MacConkey Sorbitol agar (Becton Dickinson, Franklin Lakes, NJ, USA) with colistin (200 U ml-1; MP Biomedicals, Aurora, OH, USA) (MCSA) . Inoculated MCSA plates were incubated overnight at 37°C.
The number of coral fragments collected per coral colony ranged from one to four (Table 1). Within three hours of collection, these fragments were distributed into aquaria, three fragments per aquarium, with the goal of limiting replication of fragments from any one colony in a single aquarium. For colonies from which more than one fragment was collected, this distribution of fragments allowed for genotype comparisons across treatments (Table 1). Clonal replication within an aquarium was measured in aquariums 4 and 10 (Table 2) to determine genotype response to a single treatment. Coral fragments acclimated in seawater aquaria for 48 hours prior to commencement of inoculation experiments.
Challenge Experiment Inoculations
Test isolates used in the challenge experiments were collected between June 2002 and August 2006 from off-shore coral reefs and wastewater in the Florida Keys (Table 3). These Serratia marcescens isolates were cultured and identified from environmental samples by Sutherland et al.  using a three-step method: culture on MCSA followed by culture on DNase with Toluidine Blue agar (DTC; Becton Dickinson) amended with cephalothin (0.1 mg ml−1; ICN Biomedicals, Aurora, OH, USA)  followed by Serratia-specific PCR with primers Smar 16SV (GGGAGCTTGCTCACTGGGTG) and Smar 16SWR (GCGAGTAACGTCAGTTGATGAGCGTATTA) (Sigma-Genosys, Woodlands, TX) . The efficiency of these methods for the isolation of S. marcescens was confirmed with sequencing of 16S rDNA PCR amplicons . Genotypic fingerprinting (by endonuclease restriction) of environmental isolates of S. marcescens was conducted with pulsed-field gel electrophoresis (PFGE), based on the protocols of Miranda et al.  and Shi et al. , in order to determine relatedness between isolates and to assess genetic similarity among and between strains . PFGE is widely recognized as an outstanding method for molecular strain typing of S. marcescens and is commonly employed to source track clinical outbreaks caused by this bacterium , , –.
Six of the test isolates used in the challenge experiments were a single PFGE-identified strain, Serratia marcescens PDR60, collected from APS-affected Acropora palmata at Rock Key reef in the lower Keys (isolate 1) and Grecian Rocks reef in the upper Keys (isolates 2, 3), the apparently healthy non-host coral Siderastrea siderea (isolate 4) at Long Key, the corallivorous snail Coralliophila abbreviata (isolate 5) at Sand Key reef, and from untreated wastewater (isolate 6) from the Key West wastewater treatment facility (Table 3) . The known coral pathogenic strain, S. marcescens PDL100 (isolate 7)  and one additional isolate, representing a different S. marcescens strain, WWI31, from untreated wastewater (isolate 8)  were also tested (Table 3). Controls included Escherichia coli (ATCC 15597), vehicle (sterile CaCO3 sediment), and seawater. One aquarium containing three coral fragments was used for each treatment and each control (Table 2). Due to the threatened status of A. palmata, the scope of our study was limited to eight treatments and three controls, each in a single dose, by the small size and low number of coral fragments that were permitted for collection.
Bacterial cultures of test isolates were prepared by combining 15 ml trypticase soy (TS) broth (Becton Dickinson, Sparks, MD, USA) and 15 g sterile sieved (1 mm internal diameter) CaCO3 sediment in sterile 50 ml tubes and inoculating each broth-sediment mixture with a test isolate. Vehicle control was prepared as above, but without bacterial inoculum. Inoculated mixtures were incubated overnight (25°C) with horizontal shaking and grown to 107 colony forming units ml−1. Overnight cultures were centrifuged (5 min, 1000× g) and supernatant discarded. Sterile artificial seawater (ASW, 15 ml) was added to the pellet containing cells and sediment and mixed by inversion. Inoculations were performed by depositing 0.85 g of each bacterial absorbed sediment mixture directly onto triplicate fragments in a single aquarium. Fragments that did not develop disease signs within seven days (Table 2) were inoculated again with fresh bacterial absorbed sediment. Fragments were photographed immediately before and after inoculation.
The number of days to tissue loss for each coral fragment was calculated from the first experimental inoculation (Table 2). When tissue loss was observed, the fragment was photographed and SML (containing necrotic coral tissue) was collected from the lesion margin using a sterile needle-less 20 cm3 syringe. SML was transferred to a sterile 15 ml plastic tube and centrifuged (5 min, 1000× g) and supernatant discarded. The pellet was resuspended in ASW (500 ml) and spread plated (100 µl) in triplicate on MCSA. Inoculated MCSA plates were incubated overnight at 37°C. MCSA positive bacterial colonies, appearing pink to red on MCSA (characteristic of Serratia marcescens), were picked and plated onto DTC. Inoculated DTC plates were incubated overnight at 41°C. Presumptive S. marcescens, determined by a DNase-positive reaction (appearance of a red halo on DTC), were streaked onto non-selective TS agar and incubated overnight at 25°C for confirmation of pure colonies. Experiments were monitored several times daily. Partial water changes were conducted at least once daily and salinity was maintained at 35 (measured by refractometry). After 26 days experiments were concluded. All fragments remaining alive were apparently healthy. Each fragment was photographed and then SML was collected and processed as described above. At the conclusion of experiments, or after development of disease signs and examination of SML, each Acropora palmata fragment was fixed with Z-Fix concentrate (ANATECH, LTD, Battle Creek, MI, USA) diluted 1∶4 with ASW and stored for future study.
Strain Typing of APS Pathogens
Isolates recovered from APS-affected Acropora palmata fragments in inoculation experiments and identified as presumptive Serratia marcescens by differential growth on MCSA and DTC were assayed with Serratia-specific PCR as described above and in Sutherland et al.  to confirm the identity of isolates as S. marcescens. PCR-confirmed S. marcescens were assayed with PFGE as described in Sutherland et al. . Band patterns were used to determine relatedness between isolates in order to confirm that bacteria collected from APS lesions produced during inoculation experiments were the same strain of bacterium inoculated in each inoculation experiment, thereby fulfilling Koch's postulates.
Serratia marcescens were not identified from SML of any Acropora palmata fragments prior to use in challenge experiments or from any apparently healthy fragments remaining alive at the end of the study. Seawater circulating around aquaria, seawater used for water changes in aquaria, and deionized water used to adjust salinity were all negative for S. marcescens. A. palmata fragments in the E. coli-plus-vehicle control, the seawater-alone control, and the isolate 3-plus-vehicle treatment aquaria remained apparently healthy and were maintained for the duration of the 26 day study. In the vehicle control aquarium, coral tissue loss was noted by day 15 (Table 2). Therefore, test inocula were considered virulent if disease signs occurred prior to day 15, or within 14 days of the first inoculation, and attenuated if disease signs developed after 14 days.
When disease occurred, the process began with the distinct smell of Acropora palmata mucus followed by decreased water clarity, presumably due to the release of mucus by the coral into the water. Seawater in the affected aquaria was changed every two to five hours once these signs developed. Mucus release was followed by a tissue bleb adjacent the region of inoculation. Finally, the tissue lifted off the skeleton and was sloughed in stringy fragments. This process of disease progression, once begun, was completed within 24 hours.
Koch's postulates were satisfied for Serratia marcescens strain PDR60 from APS-affected corals (isolates 1, 2). Acropora palmata fragments also developed APS signs when inoculated with two S. marcescens strains from wastewater [strain PDR60 (isolate 6) and strain WWI31 (isolate 8)] and with strain PDR60 from healthy non-host corals (isolate 4) and snails (isolate 5); however, individual strains showed varying degrees of pathogenic effect. Strain PDR60 from APS-affected corals and wastewater (isolates 1, 2, 6) caused disease signs within four to ten days (Table 2, Fig. 1). Strain WWI31 (isolate 8) from wastewater caused disease signs within 12 days. Isolate 4 (strain PDR60 from Siderastrea siderea) and strain PDL100 (isolate 7), were attenuated, and only caused disease signs at 23 and 19 days, respectively (Table 2, Fig. 1). PFGE analyses demonstrated that the original inocula were recovered from all APS lesions.
Serratia marcescens strain PDR60 isolated from wastewater and diseased Acropora palmata caused APS in as little as four days, confirming humans as a source of this disease. Although the scope of this study was limited to three replicate A. palmata fragments in a single aquarium for each treatment and each control, our results with strain PDR60 from wastewater, predatory snails, and non-host corals contribute to the understanding of the mechanisms of transmission of APS and of the factors that drive the emergence and maintenance of this marine epizootic.
Acropora palmata inoculated with strain PDR60 from the non-host coral Siderastrea siderea (isolate 4) and strain PDL100 from APS-affected host corals (isolate 7) showed an attenuated pathogenic affect (Fig. 1). These two isolates caused APS signs after the fragments in the vehicle control showed tissue loss. All other treatments that exhibited tissue loss, did so by day 13, two days before the vehicle control (Table 2). For this reason, results for isolates 4 and 7, may indicate that stressors other than Serratia marcescens infection contributed to tissue loss; however, the maintenance of the E. coli-plus-vehicle control and the isolate 3-plus-vehicle treatment for the duration of the 26 day study lends support to the pathogenic effects of isolate 4 and isolate 7. Results with isolate 4 indicate that S. siderea, and possibly other non-host corals (e.g., Solenastrea bournoni) , may serve as a reservoir for the acroporid pathogen in the reef environment. Previous work suggests that PDL100 is poorly adapted for survival in the marine environment, persisting in seawater for only 15 h , but survival and proliferation of this strain is enhanced when it is grown in A. palmata SML and S. siderea SML , . Our results indicate that PDL100 survived for at least 11 days in association with A. palmata; PDL100 was recovered from an APS lesion on day 19 of the experiment (Table 2), 11 days after the second experimental inoculation. S. marcescens resident in non-host SML may be transmitted to neighboring A. palmata by sloughing of SML or movement by Coralliophila abbreviata vectors .
Coralliophila abbreviata preys preferentially on Acropora palmata  and has been implicated in the transmission of an unknown disease of Acropora cervicornis . Strain PDR60 from C. abbreviata (isolate 5) was virulent, but only in one of three fragments (Table 2). Colony 13, a colony for which genotype comparisons across treatments were conducted, did not develop APS signs when inoculated with isolate 5 (Table 2) or with any other treatment (Table 1). The limited virulence of isolate 5 may be due to the apparent APS resistance of colony 13 or to the specific origin of this isolate, collected in 2006 at Sand Key reef when no sign of white pox was apparent at that reef . These experimental and field observations support the limited virulence of isolate 5, but virulence of this isolate against one A. palmata fragment is consistent with the hypothesis that C. abbreviata may function as an interepizootic reservoir of the APS pathogen or as a vector when snails that harbor the pathogen feed on host corals.
Acropora palmata inoculated with isolate 3 from APS-affected corals remained apparently healthy for the entirety of the experiment, indicating that either this isolate of strain PDR60 is non-virulent or that the corals on to which it was inoculated were resistant to APS. Evidence in support of the former conclusion is that the three A. palmata fragments inoculated with isolate 3 were each a different genotype and all remained apparently healthy for the duration of the 26 day study (Table 2). The later conclusion is supported by the apparent APS resistance of colony 13 (Table 1). Observations of A. palmata populations suggest that colonies of this species that are resistant to APS may exist. Prevalence of white pox disease has declined in recent years coincident with declines in host coral populations. The most recent reported outbreaks of the disease occurred in the Florida Keys in 2003  and 2009 (Fig. 1) and in St. John, US Virgin Islands in 2006 . Lower disease prevalence may be due to the decimation of susceptible A. palmata populations during previous white pox epizootics , , other disease outbreaks , , or may be an indication of reduced pathogen virulence .
Serratia marcescens strain PDL100 exhibited attenuated virulence in this study, and this strain's reduced pathogenic effect, combined with the virulence of strain PDR60 and the wastewater strain WWI31, raises the question of whether unique strains exist for other coral pathogens. For instance, coral disease pathogens that cause white plague (Aurantimonas corallicida) and bacterial-induced bleaching (Vibrio shiloi) have waned in pathogenicity in recent years . Limited pathogenicity of A. corallicida, V. shiloi, and S. marcescens PDL100 may indicate the evolution of host-resistance to these pathogens .
We have shown that distinct strains of Serratia marcescens are capable of causing disease in Acropora palmata and that strains show varying levels of apparent virulence with time to disease lesions ranging from 4 days to 23 days (Table 2). These results may explain variability in manifestation of APS lesions on A. palmata colonies in the field including time to infection, rate of tissue loss, and likelihood of recovery versus whole-colony death. Future studies will investigate host susceptibility and resistance and will evaluate genetic variation between S. marcescens isolates and strains confirmed to be pathogenic and those that do not cause disease or cause disease at a slower rate. There is a need to define key differences between S. marcescens strains (PDL100 and PDR60) as well as among isolates of the same strain (PDR60, e.g., isolates 1–6). Important differences may exist in their ability to adapt to marine conditions (i.e., ability to persist and/or grow in seawater) and in genetic differences leading to increased virulence. S. marcescens vary widely in their survival in seawater with strain PDL100 surviving for 15 h or less at 30°C  and marine-derived isolates of the PDR60 pathogen strain persisting for up to 20 days under the same conditions (KP Sutherland and EK Lipp unpublished data). Fitness of different S. marcescens strains in seawater may also shed light on the mode of APS transmission. For example, saltwater tolerant strains may be more likely to become persistent or even resident on the reef whereas less halo-tolerant strains may either die off before reaching the reef or may be responsible for discrete outbreaks over short time periods. Our data from microcosm studies suggest that PDL100 (isolated in 1999) follows the latter pattern  and is supported by the fact that this strain has never been re-isolated from the marine environment . On the other hand, strain PDR60, which has been recovered from multiple marine sources , is highly persistent in marine waters (KP Sutherland and EK Lipp unpublished data), suggesting that this may be an endemic disease strain.
Human culpability in the demise of a threatened species necessitates an immediate response and supports ongoing mitigation to improve wastewater treatment in the Florida Keys, and elsewhere, in order to protect the health and biodiversity of coral reef ecosystems. Advanced wastewater treatment successfully removes Serratia marcescens to undetectable levels . However, most wastewater in the Keys and wider Caribbean is not treated, but rather is disposed of through in-ground receptacles  within porous limestone substrate that permits leakage from these systems into near-shore waters . Human fecal contamination of near-shore and off-shore coral reef environments has been clearly demonstrated in the Florida Keys – and elsewhere in the Caribbean  and is associated with waterborne disease in humans . In response, the state of Florida passed legislation to improve water quality in the Florida Keys by requiring the upgrade of all wastewater facilities, including in-ground receptacles, to the best available technology or to advanced wastewater treatment at an estimated cost of $939 million .
Coral reefs are amongst the most critically endangered habitats on earth. Because population declines of Acropora palmata are caused, in part, by a human strain of the common fecal enteric bacterium, Serratia marcescens, our findings address not only environmental protection, but also the socioeconomic and socio-ecological determinants of coastal zone protection and the cost of wastewater treatment infrastructure. This study brings to light a disease system dynamic, from humans to wildlife, which is the opposite of the traditional wildlife-to-human disease transmission model. The passage of pathogens from wildlife to humans is well documented, but the movement of disease-causing microbes from humans to marine invertebrates has not been shown . This “reverse zoonosis” is all the more interesting because it involves the jump of a pathogen from vertebrate to invertebrate and from terrestrial to marine. Furthermore, disease incidence or severity may increase with rising temperatures , , , reinforcing the importance of near-shore water quality under climate change scenarios. Given a reliance on tourism by most Caribbean countries and the widespread lack of consistent wastewater treatment in the region, the APS disease system is of great economic interest and public health concern to developing nations and has particular significance for sustainable development activities and coastal-zone carrying capacity studies world-wide, especially under changing climatic conditions.
We thank the Mote Tropical Research Laboratory, Summerland Key, FL for boat support, field assistance, and facilities. Research at Florida Keys National Marine Sanctuary was conducted under permit no. FKNMS-2009-050.
Conceived and designed the experiments: KPS EKL JWP. Performed the experiments: KPS SS JLJ EKL. Analyzed the data: KPS SS EKL. Wrote the paper: KPS SS EKL JWP.
- 1. Hogarth WT (2006) Endangered and threatened species: final listing determinations for elkhorn coral and staghorn coral. Fed Register 71(89): 26852–26861.WT Hogarth2006Endangered and threatened species: final listing determinations for elkhorn coral and staghorn coral.Fed Register71892685226861
- 2. Patterson KL, Porter JW, Ritchie KB, Polson SW, Mueller E, et al. (2002) The etiology of white pox, a lethal disease of the Caribbean elkhorn coral, Acropora palmata. Proc Natl Acad Sci USA 99: 8725–8730.KL PattersonJW PorterKB RitchieSW PolsonE. Mueller2002The etiology of white pox, a lethal disease of the Caribbean elkhorn coral, Acropora palmata.Proc Natl Acad Sci USA9987258730
- 3. Graber CD, Higgins LS, Davis JS (1965) Seldom-encountered agents of bacterial meningitis. JAMA 192: 956–960.CD GraberLS HigginsJS Davis1965Seldom-encountered agents of bacterial meningitis.JAMA192956960
- 4. Magnuson EW, Elston HR (1966) Infections caused by non-pigmented Serratia marcescens. Ann Intern Med 65: 409–418.EW MagnusonHR Elston1966Infections caused by non-pigmented Serratia marcescens.Ann Intern Med65409418
- 5. Ringrose RE, McKown B, Felton FG, Barclay BO, Muchmore HG, et al. (1968) A hospital outbreak of Serratia marcescens associated with ultrasonic nebulizers. Ann Intern Med 69: 719–729.RE RingroseB. McKownFG FeltonBO BarclayHG Muchmore1968A hospital outbreak of Serratia marcescens associated with ultrasonic nebulizers.Ann Intern Med69719729
- 6. Cabrera HA (1969) An outbreak of Serratia marcescens and its control. Arch Intern Med 123: 650–655.HA Cabrera1969An outbreak of Serratia marcescens and its control.Arch Intern Med123650655
- 7. Tillotson JR, Finland M (1969) Bacterial colonization and clinical superinfection of the respiratory tract complicating antibiotic treatment and pneumonia. J Infec Dis 119: 597–624.JR TillotsonM. Finland1969Bacterial colonization and clinical superinfection of the respiratory tract complicating antibiotic treatment and pneumonia.J Infec Dis119597624
- 8. Miranda G, Kelly C, Solorzano F, Leanos B, Coria R, et al. (1996) Use of pulsed-field gel electrophoresis typing to study an outbreak of infection due to Serratia marcescens in a neonatal intensive care unit. J Clin Microbiol 34: 3138–3141.G. MirandaC. KellyF. SolorzanoB. LeanosR. Coria1996Use of pulsed-field gel electrophoresis typing to study an outbreak of infection due to Serratia marcescens in a neonatal intensive care unit.J Clin Microbiol3431383141
- 9. Shi ZY, Liu PYF, Lin YH, Hu BS (1997) Use of pulsed-field gel electrophoresis to investigate an outbreak of Serratia marcescens. J Clin Microbiol 35: 325–327.ZY ShiPYF LiuYH LinBS Hu1997Use of pulsed-field gel electrophoresis to investigate an outbreak of Serratia marcescens.J Clin Microbiol35325327
- 10. Grimont F, Grimont PAD (2006) The Genus Serratia. In: Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E, editors. The Prokaryotes, vol. 6. New York: Springer. pp. 219–244.F. GrimontPAD Grimont2006The Genus Serratia.M. DworkinS. FalkowE. RosenbergK-H SchleiferE. StackebrandtThe Prokaryotes, vol. 6New YorkSpringer219244
- 11. Hazen TC (1988) Fecal coliforms as indicators in tropical waters: a review. Tox Assess 3: 461–477.TC Hazen1988Fecal coliforms as indicators in tropical waters: a review.Tox Assess3461477
- 12. Hejazi A, Falkiner FR (1997) Serratia marcescens. J Med Microbiol 46: 903–912.A. HejaziFR Falkiner1997Serratia marcescens.J Med Microbiol46903912
- 13. Sutherland KP, Porter JW, Turner JW, Thomas BJ, Looney EE, et al. (2010) Human sewage identified as likely source of white pox disease of the threatened Caribbean elkhorn coral, Acropora palmata. Environ Microbiol 12: 1122–1131.KP SutherlandJW PorterJW TurnerBJ ThomasEE Looney2010Human sewage identified as likely source of white pox disease of the threatened Caribbean elkhorn coral, Acropora palmata.Environ Microbiol1211221131
- 14. Sutherland KP, Porter JW, Torres C (2004) Disease and immunity in Caribbean and Indo-Pacific zooxanthellate corals. Mar Ecol Prog Ser 266: 273–302.KP SutherlandJW PorterC. Torres2004Disease and immunity in Caribbean and Indo-Pacific zooxanthellate corals.Mar Ecol Prog Ser266273302
- 15. Grasso GM, D'Errico MM, Schioppa F, Romano F, Montanaro D (1988) Use of colistin and sorbitol for better isolation of Serratia marcescens in clinical samples. Epidemiol Infec 101: 315–320.GM GrassoMM D'ErricoF. SchioppaF. RomanoD. Montanaro1988Use of colistin and sorbitol for better isolation of Serratia marcescens in clinical samples.Epidemiol Infec101315320
- 16. Farmer JJ, Silva F, Williams DR (1973) Isolation of Serratia marcescens on deoxyribonuclease-toluidine blue-cephalothin agar. Appl Microbiol 25: 151–152.JJ FarmerF. SilvaDR Williams1973Isolation of Serratia marcescens on deoxyribonuclease-toluidine blue-cephalothin agar.Appl Microbiol25151152
- 17. Wilson VL, Tatford BC, Yin X, Rajki SS, Walsh MM, et al. (1999) Species-specific detection of hydrocarbon-utilizing bacteria. J Microbiol Meth 39: 59–78.VL WilsonBC TatfordX. YinSS RajkiMM Walsh1999Species-specific detection of hydrocarbon-utilizing bacteria.J Microbiol Meth395978
- 18. Alaidan A, Bohol MF, Al-Thawadi SI, Al-Suwaine A, Al-Qahtani AA, et al. (2009) Rapid PFGE method for fingerprinting of Serratia marcescens isolates. J Microbiol Meth 78: 238–241.A. AlaidanMF BoholSI Al-ThawadiA. Al-SuwaineAA Al-Qahtani2009Rapid PFGE method for fingerprinting of Serratia marcescens isolates.J Microbiol Meth78238241
- 19. Alfizah H, Nordiah AJ, Rozaidi WS (2004) Using pulsed-field gel electrophoresis in the molecular investigation of an outbreak of Serratia marcescens infection in an intensive care unit. Singapore Med J 45: 214–218.H. AlfizahAJ NordiahWS Rozaidi2004Using pulsed-field gel electrophoresis in the molecular investigation of an outbreak of Serratia marcescens infection in an intensive care unit.Singapore Med J45214218
- 20. Maragakis LL, Winkler A, Tucker MG, Cosgrove SE, Ross T, et al. (2008) Outbreak of multidrug-resistant Serratia marcescens infection in a neonatal intensive care unit. Infect Control Hosp Epidemiol 29: 418–423.LL MaragakisA. WinklerMG TuckerSE CosgroveT. Ross2008Outbreak of multidrug-resistant Serratia marcescens infection in a neonatal intensive care unit.Infect Control Hosp Epidemiol29418423
- 21. Milisavljevic V, Wu F, Larson E, Rubenstein D, Ross B, et al. (2004) Molecular epidemiology of Serratia marcescens outbreaks in two neonatal intensive care units. Infect Control Hospital Epidemiol 25: 719–721.V. MilisavljevicF. WuE. LarsonD. RubensteinB. Ross2004Molecular epidemiology of Serratia marcescens outbreaks in two neonatal intensive care units.Infect Control Hospital Epidemiol25719721
- 22. van der Vorm ER, Woldring-Zwaan C (2002) Source, carriers, and management of a Serratia marcescens outbreak on a pulmonary unit. J Hosp Infect 52: 263–267.ER van der VormC. Woldring-Zwaan2002Source, carriers, and management of a Serratia marcescens outbreak on a pulmonary unit.J Hosp Infect52263267
- 23. Steppberger K, Walter S, Claros MC, Spencker FB, Kiess W, et al. (2002) Nosocomial neonatal outbreak of Serratia marcescens – analysis of pathogens by pulsed-field gel electrophoresis and polymerase chain reaction. Infection 30: 277–281.K. SteppbergerS. WalterMC ClarosFB SpenckerW. Kiess2002Nosocomial neonatal outbreak of Serratia marcescens – analysis of pathogens by pulsed-field gel electrophoresis and polymerase chain reaction.Infection30277281
- 24. Ligozzi M, Fontana R, Aldegheri M, Scalet G, Lo Cascio G (2010) Comparative evaluation of an automated repetitive-sequence-based PCR instrument versus pulsed-field gel electrophoresis in the setting of Serratia marcescens nosocomial infection outbreak. J Clin Microbiol 48: 1690–1695.M. LigozziR. FontanaM. AldegheriG. ScaletG. Lo Cascio2010Comparative evaluation of an automated repetitive-sequence-based PCR instrument versus pulsed-field gel electrophoresis in the setting of Serratia marcescens nosocomial infection outbreak.J Clin Microbiol4816901695
- 25. Bayramoglu G, Buruk K, Dinc U, Mutlu M, Yilmaz G, et al. (2011) Investigation of an outbreak of Serratia marcescens in a neonatal intensive care unit. J Microbiol Immunol 44: 111–115.G. BayramogluK. BurukU. DincM. MutluG. Yilmaz2011Investigation of an outbreak of Serratia marcescens in a neonatal intensive care unit.J Microbiol Immunol44111115
- 26. Looney EE, Sutherland KP, Lipp EK (2010) Effects of temperature, nutrients, organic matter and coral mucus on the survival of the coral pathogen, Serratia marcescens PDL100. Environ Microbiol 12: 2479–2485.EE LooneyKP SutherlandEK Lipp2010Effects of temperature, nutrients, organic matter and coral mucus on the survival of the coral pathogen, Serratia marcescens PDL100.Environ Microbiol1224792485
- 27. Krediet CJ, Ritchie KB, Cohen M, Lipp E, Sutherland KP, et al. (2009) Utilization of mucus from the coral Acropora palmata by the pathogen Serratia marcescens, environmental and coral commensal bacteria. Appl Environ Microbiol 7: 3851–3858.CJ KredietKB RitchieM. CohenE. LippKP Sutherland2009Utilization of mucus from the coral Acropora palmata by the pathogen Serratia marcescens, environmental and coral commensal bacteria.Appl Environ Microbiol738513858
- 28. Miller MW (2001) Corallivorous snail removal: evaluation of impact on Acropora palmata. Coral Reef 19: 293–295.MW Miller2001Corallivorous snail removal: evaluation of impact on Acropora palmata.Coral Reef19293295
- 29. Williams DE, Miller MW (2005) Coral disease outbreak: pattern, prevalence and transmission in Acropora cervicornis. Mar Ecol Prog Ser 301: 119–128.DE WilliamsMW Miller2005Coral disease outbreak: pattern, prevalence and transmission in Acropora cervicornis.Mar Ecol Prog Ser301119128
- 30. Muller EM, Rogers CS, Spitzack AS, van Woesik R (2008) Bleaching increases likelihood of disease on Acropora palmata (Lamarck) in Hawksnest Bay St. John, US Virgin Islands. Coral Reefs 27: 191–195.EM MullerCS RogersAS SpitzackR. van Woesik2008Bleaching increases likelihood of disease on Acropora palmata (Lamarck) in Hawksnest Bay St. John, US Virgin Islands.Coral Reefs27191195
- 31. Rodríguez-Martínez RE, Banaszak AT, Jordán-Dahlgren E (2001) Necrotic patches affect Acropora palmata (Scleractinia: Acroporidae) in the Mexican Caribbean. Dis Aquat Org 47: 229–234.RE Rodríguez-MartínezAT BanaszakE. Jordán-Dahlgren2001Necrotic patches affect Acropora palmata (Scleractinia: Acroporidae) in the Mexican Caribbean.Dis Aquat Org47229234
- 32. Gladfelter WB (1982) White-band disease in Acropora palmata: implications for the structure and growth of shallow reefs. Bull Mar Sci 32: 639–643.WB Gladfelter1982White-band disease in Acropora palmata: implications for the structure and growth of shallow reefs.Bull Mar Sci32639643
- 33. Aronson RB, Precht WF (2001) White-band disease and the changing face of Caribbean coral reefs. Hydrobiologia 460: 25–38.RB AronsonWF Precht2001White-band disease and the changing face of Caribbean coral reefs.Hydrobiologia4602538
- 34. Reschef L, Koren O, Loya Y, Zilber-Rosenberg I, Rosenberg E (2006) The coral probiotic hypothesis. Environ Microbiol 8: 2068–2073.L. ReschefO. KorenY. LoyaI. Zilber-RosenbergE. Rosenberg2006The coral probiotic hypothesis.Environ Microbiol820682073
- 35. Paul JH, Rose JB, Jiang SC, Zhou X, Cochran P, et al. (1997) Evidence for groundwater and surface marine water contamination by waste disposal wells in the Florida Keys. Water Res 31: 1448–1454.JH PaulJB RoseSC JiangX. ZhouP. Cochran1997Evidence for groundwater and surface marine water contamination by waste disposal wells in the Florida Keys.Water Res3114481454
- 36. Lapointe BE, O'Connell JD, Garrett GS (1990) Nutrient coupling between on-site sewage disposal systems groundwaters and nearshore surface waters of the Florida Keys. Biogeochemistry 10: 289–307.BE LapointeJD O'ConnellGS Garrett1990Nutrient coupling between on-site sewage disposal systems groundwaters and nearshore surface waters of the Florida Keys.Biogeochemistry10289307
- 37. Griffin DW, Gibson CJ 3rd, Lipp EK, Riley K, Paul JH, et al. (1999) Detection of viral pathogens by reverse transcriptase PCR and of microbial indicators by standard methods in the canals of the Florida Keys. Appl Environ Microbiol 65: 4118–4125.DW GriffinCJ Gibson 3rdEK LippK. RileyJH Paul1999Detection of viral pathogens by reverse transcriptase PCR and of microbial indicators by standard methods in the canals of the Florida Keys.Appl Environ Microbiol6541184125
- 38. Futch JC, Griffin DW, Lipp EK (2010) Human enteric viruses in groundwater indicate offshore transport of human sewage to coral reefs of the upper Florida Keys. Environ Microbiol 12: 964–974.JC FutchDW GriffinEK Lipp2010Human enteric viruses in groundwater indicate offshore transport of human sewage to coral reefs of the upper Florida Keys.Environ Microbiol12964974
- 39. Bonkosky M, Hernandez-Delgado EA, Sandoz B, Robledo IE, Norat-Ramirez J, et al. (2009) Detection of spatial fluctuations of non-point source fecal pollution in coral reef surrounding waters in southwestern Puerto Rico using PCR-based assays. Mar Poll Bull 58: 45–54.M. BonkoskyEA Hernandez-DelgadoB. SandozIE RobledoJ. Norat-Ramirez2009Detection of spatial fluctuations of non-point source fecal pollution in coral reef surrounding waters in southwestern Puerto Rico using PCR-based assays.Mar Poll Bull584554
- 40. Nobles RE, Brown P, Rose JB, Lipp EK (2000) The investigation and analysis of swimming-associated-illness using the fecal indicator enterococcus in southern Florida's marine waters. Florida J Environ Health 169: 15–19.RE NoblesP. BrownJB RoseEK Lipp2000The investigation and analysis of swimming-associated-illness using the fecal indicator enterococcus in southern Florida's marine waters.Florida J Environ Health1691519
- 41. CH2MHILL (2000) Monroe County Sanitary Wastewater Master Plan. Monroe County, FL. CH2MHILL2000Monroe County Sanitary Wastewater Master Plan. Monroe County, FL.Available: http://www.monroecounty-fl.gov/DocumentView.aspx?DID=477. Accessed: 2011 Aug 08. Available: http://www.monroecounty-fl.gov/DocumentView.aspx?DID=477. Accessed: 2011 Aug 08.
- 42. Woolhouse M, Gaunt E (2007) Ecological origins of novel human pathogens. Crit Rev Microbiol 33: 231–242.M. WoolhouseE. Gaunt2007Ecological origins of novel human pathogens.Crit Rev Microbiol33231242
- 43. Harvell CD, Kim K, Burkholder JM, Colwell RR, Epstein PR, et al. (1999) Emerging marine diseases- climate links and anthropogenic factors. Science 285: 1505–1510.CD HarvellK. KimJM BurkholderRR ColwellPR Epstein1999Emerging marine diseases- climate links and anthropogenic factors.Science28515051510
- 44. Ritchie KB (2006) Regulation of microbial populations by coral surface mucus-associated bacteria. Mar Ecol Prog Ser 322: 1–14.KB Ritchie2006Regulation of microbial populations by coral surface mucus-associated bacteria.Mar Ecol Prog Ser322114