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Mapping Salinity Tolerance during Arabidopsis thaliana Germination and Seedling Growth

  • Leah DeRose-Wilson,

    Current address: Department of Bio-Agriculture Sciences and Pest Management, Colorado State University, Fort Collins, Colorado, United States of America

    Affiliation Department of Ecology and Evolutionary Biology, University of California Irvine, Irvine, California, United States of America

  • Brandon S. Gaut

    bgaut@uci.edu

    Affiliation Department of Ecology and Evolutionary Biology, University of California Irvine, Irvine, California, United States of America

Mapping Salinity Tolerance during Arabidopsis thaliana Germination and Seedling Growth

  • Leah DeRose-Wilson, 
  • Brandon S. Gaut
PLOS
x

Abstract

To characterize and dissect genetic variation for salinity tolerance, we assessed variation in salinity tolerance during germination and seedling growth for a worldwide sample of Arabidopsis thaliana accessions. By combining QTL mapping, association mapping and expression data, we identified genomic regions involved in salinity response. Among the worldwide sample, we found germination ability within a moderately saline environment (150 mM NaCl) varied considerable, from >90% among the most tolerant lines to complete inability to germinate among the most susceptible. Our results also demonstrated wide variation in salinity tolerance within A. thaliana RIL populations and identified multiple genomic regions that contribute to this variation. These regions contain known candidate genes, but at least four of the regions contain loci not yet associated with salinity tolerance response phenotypes. Our observations suggest A. thaliana natural variation may be an underutilized resource for investigating salinity stress response.

Introduction

Abiotic factors like temperature, drought and salinity are often temporally and spatially heterogeneous. Because plants are sessile and cannot escape environmental challenges, abiotic stresses are likely to cause selective pressures that lead to local adaptation. Indeed, plants have evolved complex physiological mechanisms to respond to abiotic stresses [1][3], many of which are shared among different stress factors [4][8]. Variation in water use efficiency and response to drought stress within and between populations has been studied for a number of species; however in most of these cases it is not currently feasible to understand the underlying genetics of this variation [9][12].

Soil salinity is a particularly pernicious abiotic stress, both because it is an agricultural problem (>20% of arable land worldwide is currently affected by irrigation-induced salinification; [13][14]), and because it fundamentally disrupts plant physiology [15]. Salinity stress is commonly separated into two components: ionic stress and osmotic stress [16], [3]. Plants respond to ionic stress by active exclusion of salt ions or by shunting salt ions into storage tissues in order to maintain cellular homeostasis. Osmotic stress arises because the presence of salts (usually Na+) affects a plant's ability to absorb water and thus limits water availability to plant tissues. The plant response to osmotic stress is physiologically similar to the response to drought stress; ultimately both responses lead to the accumulation of osmolytes and other compounds [17][20], [6], [8], often via regulation by abscisic acid (ABA) signaling [21], [22]. If the initial response to either ionic or osmotic stress is insufficient, plants have several mechanisms to limit salt damage, including developmental modifications and the production of hormones and anti-oxidative enzymes [23][26], [15].

Relatively little is known about the specific genetic mechanisms underlying adaptation to abiotic stresses [27][29], [6]. Most efforts to elucidate the molecular basis of salinity tolerance have employed mutagenesis screens in model organisms. These studies have yielded a number of candidate genes and candidate pathways for the evolution of salinity tolerance. One important example is the Na+/H+ ion antiporter AtNHX1, which has been cloned in Arabidopsis thaliana and used to create transgenic tomato (Solanum lycopersicum) and canola (Brassica napus) lines [30][31]. These lines are able to grow in high (200 mM) NaCl, a concentration usually untenable for wild-type plant growth. Other genes and pathways conferring salt tolerance have been identified, such as genes in the salt overly sensitive (or SOS) pathway [32][33], genes involved in ABA signaling or synthesis [34][36], and other genes with an array of functions [37][41].

The large number of genes implicated in salinity tolerance may not be surprising, because many of the traits underlying adaptation to abiotic stress are quantitative and controlled by perhaps hundreds of genes and dozens of genetic pathways [42][44]. While several studies have investigated the molecular basis of salinity tolerance in A. thaliana [45][46], [4], [19], little attention has been paid to measuring naturally occurring quantitative variation in salinity tolerance and other abiotic stresses in this model species (but see: [47][50]). One recent exception is the study of the sodium transporter AtHKT1; allelic variation at this locus was found to regulate sodium accumulation along a coastal cline of A. thaliana accessions collected across Europe [51]. However, genetic variation correlated with salinity tolerance and other abiotic stresses has been investigated in a number of other species more thoroughly [52][53], most notably salinity in Helianthus [54]. Still, in most cases candidate loci have not been identified, making it difficult to characterize the genetic basis of abiotic stress tolerance.

Here we assess natural variation for salinity tolerance in A. thaliana and utilize both association and QTL mapping to identify candidate genes that could be contributing to variation in salinity tolerance. In part because of the physiological complexity of the salinity response, there have been few studies looking for QTL for this trait in A. thaliana (but see [49] and [36]). There has been, however, limited success for detecting QTL for other physiologically complex traits, such as drought [55][56], [47], cold stress [57][59] and heavy metals [60]. These studies suggest that physiologically complex traits likely contain some large effect alleles [36], [47]. While QTL approaches may be suitable for detecting rare alleles of large effect, the complementary approach of association mapping may provide additional information, because association mapping incorporates a broader range of natural variation than QTL mapping [61]. As a result, association methods may allow identification of alleles of smaller effect as well as finer mapping resolution [61][64]. We note, however, that a large-scale association study in A. thaliana recently focused on one aspect of salt tolerance (leaf tissue sodium abundance) and identified only one gene (AtHK1) [64].

To date natural variation in A. thaliana salinity tolerance during germination and seedling growth has not been broadly surveyed (but see [65] for an extensive survey of salinity tolerance during vegetative growth). Here we survey natural variation in salinity tolerance, focusing on germination rates, seedling survival, and seedling development measured on an association panel of 96 accessions and on two RIL mapping populations. By comparing QTL and association results, we identify candidate regions that may contribute to tolerance. To delimit candidate genes, we turn to comparative gene expression analyses. We show that A. thaliana has wide variation in phenotypes relating to salt tolerance, that this variation has a geographic component, and finally that the genetic architecture underlying phenotypic variation consists of several major QTL and manifold genes of smaller effect. Overall, our multifaceted approach contributes to an understanding of the genetic architecture of salinity tolerance in the broader context of natural variation.

Materials and Methods

Plant Materials and Plant Growth

We procured A. thaliana seeds for association and QTL mapping from the Arabidopsis Biological Resource Center at The Ohio State University, USA. The association panel consisted of 96 accessions that have been used in previous association and diversity studies [66][67]. These 96 include pairs of individuals from 25 natural populations as well as a worldwide survey of commonly used stock center accessions. We also acquired seed from the Col×Ler (CS1899; [68]) and Cvi×Ler (CS22000; [69]) recombinant inbred lines (RILs). Ultimately, the Col×Ler and Cvi×Ler mapping populations were represented in our study by 200 and 162 RILs, respectively. In order to mitigate maternal effects based on non-heritable nutrient provisioning, all accessions and RILs used for phenotyping were based on bulked seed samples from at least 10 mothers.

Plants for phenotyping were grown and germinated in growth chamber at 22°C with long-day (16 hour light/8 hour dark) conditions. Lighting was ∼150 uE m-2 sec-1 for a 16-h photoperiod. Seeds were germinated and grown under control and two high salinity (150 mM & 250 mM) treatments. The control treatment employed 60×15 mm petri dishes with 6 mls per dish of standard MS agar germination media adjusted to ph 5.7 with 1% sucrose (http://www.biosci.ohio-state.edu/pcmb/Facilities/abrc/handling.htm). Our high salt treatments supplemented the control media with 150 mM or 250 mM NaCl.

Each phenotypic measure for each accession was based on a sample of at least 90 total seeds, planted in three replicates of 15 seeds over two blocks that were randomized with respect to the location of the 96 accessions. Control trials were done simultaneously in non-supplemented media. One control replicate of 15 seeds was included in each block for each accession, so ultimately the control sample for each accession consisted of 60 seeds (30 seeds in each of the 150 mM and 250 mM treatments). A total of four phenotypic traits were measured for each accession: total percent germination (PG), time to 50% germination (TD50), % cotyledons fully emerged (PC) and % seedling survival at 21 days (PS). Here TD50, PC, and PS are all conditional on germination; only those seeds that germinated are included in the total number used to calculate TD50 , PC and PS. We considered seeds to be germinated once the radicle had fully emerged from the seed coat. PG was scored at 24-h intervals from the 2nd day after sowing until the score remained unchanged for five consecutive days or until 100% germination was reached up to 21 days after sowing. To normalize germination percentages, we calculated PG scores as a percent of the germination on non-supplemented control media. For PS, seedlings that were yellowed, swollen or in obvious acute distress were not recorded.

Association Mapping

Association mapping analysis used the most recent release from the Arabidopsis Hap Map data available (http://walnut.usc.edu/2010/data/250k-data-version-3.03). These data include over 240,000 SNPs.

The qqnorm function with Shapiro-Wilk's test, as implemented in R, was used to determine whether the phenotype distribution fit to normality [70]. Both the PC and PS phenotypes had negatively skewed distributions, and the data were log transformed. Transformed data were approximately normally distributed (data not shown). A mixed model approach was used to test for statistical associations between phenotypes and genotypes in our mapping panel [71]. Our model followed Yu et al [71] in that we used both a random effect and a fixed effect to model the portion of phenotypic variation explained by population structure within our sample; this approach performs considerably better than using only a fixed effect to correct for population structure [72]. Previously Yu et al. [71] used the Q matrix generated with STRUCTURE to estimate genome-wide population structure; however it has been suggested that a principal components analysis (PCA) can be used to summarize genome-wide patterns of relatedness instead [73]. PCA analyses were performed by EIGENSTRAT [73]; we used the top six principal components for the P matrix. Our model combined PCA assignments (the fixed effect) for populations with kinship estimates (the random effect) to estimate the effect of population structure. Here we consider kinship as a random effect where the individual random deviations from the population mean are constrained by assuming that the (phenotypic) covariance between individuals is proportional to their relative relatedness (or kinship), which is estimated using genome-wide marker data. The model was implemented in the Tassel association mapping software [74]. Individual random deviations from the population mean were constrained by assuming that the (phenotypic) covariance between individuals was proportional to their relative relatedness (or kinship), which was estimated using genome-wide marker data. The vector of phenotypes, y, was modeled as:where X contains the genotypes, α is vector of allele effects to be estimated, P is a matrix of population assignments by PCA, β is a vector of population (from PCA assignments) effects, I is an identity matrix, u are the random deviates due to genome-wide relatedness, and e are random deviates due to noise. For each case where a marker had a significant association, a genomic region defined by LD estimates around this marker and the flanking non significant markers was extracted. Genes contained within this genomic region are considered candidates.

QTL mapping

We used the existing Col×Ler and Cvi×Ler RIL populations, which were genotyped previously [68], [69]. We constructed linkage maps using 243 molecular markers (26, 43, 66, 39 and 69 markers, respectively, for chromosomes 1–5) for the Cvi×Ler mapping population and 352 markers (82, 49, 80, 62 and 79 markers for chromosomes 1–5) for the Col×Ler mapping population. In the Cvi×Ler population, the markers covered 519.5 cM (>85% of the A. thaliana genome) and were spaced at intervals ranging from 0.5 to 8 cM, with their average distance being 3 cM. The Col×Ler map spanned 540 cM (∼90% of the A. thaliana genome) with markers spaced at intervals from 3–8 cM apart, averaging 4.5 cM between markers. We confirmed marker orders and re-estimated marker position using a joint maximum-likelihood algorithm implemented in the R mapping package R-qtl [75]. We eliminated any markers that were inconsistent in either order or relative position from their locations on the Columbia physical map. Genetic markers that have been anchored to the physical map for Cvi×Ler [76] and Col×Ler were used to estimate the boundaries of confidence intervals. QTL mapping was conducted on RIL means, obtained as the average of 10–12 seeds for each RIL planted in triplicate on three independently randomized blocks for a total of at least 90 observations (10 seeds × 3 pots × 3 blocks), for each RIL. Phenotypic data were transformed to improve normality prior to analyses when necessary.

Heritability (h2) was estimated using variance components calculated using the r/kinship package (http://cran.r-project.org/web/packages/kinship/index.html). The R-qtl (http://www.rqtl.org) program implemented in R (http://www.r-project.org), was used for both one and two-dimensional interval mapping in both crosses. We used the EM algorithm and the Haley-Knot regression algorithm at 1-cM steps across the genome. Both algorithms give qualitatively the same results although exact LOD peak locations and confidence intervals varied slightly depending on the mapping algorithm used. In addition to detecting main effects, Rqtl incorporates a multiple QTL model and allows epistatically interacting QTL to be independently identified. In addition a two-way ANOVA was used to test for a significant interaction between specific pairs of QTL. We used 1000 permutations of the dataset for each mapping population to empirically calculate genome wide significance thresholds for LOD scores [77], [78].

Differential Expression

In order to assess differential gene expression under salinity stress conditions we utilized previously generated gene expression data from the AtGenExpress data set for abiotic stress [79]. This dataset was generated with the Affymetrix ATH1 microarray as part of a comprehensive genome wide expression study in A. thaliana. The data were generated in side-by-side experimental series, with three biological replicates for both stressed and control plants. In the original study, plants were subjected to common abiotic stresses, such as temperature, drought, salt, high osmolarity, U-B light and wounding. Here we only consider data from the salinity stress treatment.

We compared expression levels at all seven time points separately, contrasting control and salt treatments at each time point. Expression differences were considered significant for genes classified as up regulated when the signal intensities for both treatment replicates were at least threefold higher than the signal intensities for both control replicates. Similarly, a gene was classified as down regulated when the intensities for both control replicates were at least threefold higher than those of both treatment replicates. By comparing each duplicated control array with its partner for every time point, it was determined that a fold change cut-off of ≥3 results in an average of 0.4% of genes falsely classified as up- or down regulated [79], suggesting our expression criterion are conservative.

Results

Natural Variation in Salinity Tolerance

Our four phenotypic traits [% germination (PG), % Cotyledons emergence (PC), Time to 50% germination (TD50) and % Survival (PS); see Methods] were assayed in 96 A. thaliana accessions under control conditions, without supplementation of NaCl. PG was >90% for 87 accessions, an additional 7 accessions had PG>85%, and the remaining two accessions had germination rates of 75% and 45%. Once germinated, all lines had PC and PS values >95% after 21 days. In control treatments TD50 means were 4.5 days and ranged from 2–8 days.

We also measured the four phenotypic traits in the presence of NaCl supplemented at 150 mM and 250 mM concentrations. PG varied substantially among the 96 lines on both 250 mM NaCl and 150 mM NaCl treatments (Figure 1). PG scores of >90% were obtained for 10 accessions at 150 mM and 2 accessions (Shakdara and Bay-0) at 250 mM. However, six accessions did not germinate in the 150 mM NaCl treatment, and 13 accessions did not germinate in the 250 mM treatment. An additional 18 accessions had PG values of less than 10% in the 250 mM treatment (data not shown).

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Figure 1. Phenotypes measured in 150 mM NaCl for 96 accessions.

The percentage of germination, cotyledon emergence and total seedling survival were measured, as well as the number of days to 50% germination. Days to 50% germination totals included only successfully germinating seeds. Means are based on a total of 300 seeds in 3 replicates. Error bars are the standard deviation. Accessions are grouped loosely by geographic origin: Northern Sweden (N. SWE), Southern Sweden (S. SWE), Northern Europe (N. EUR), Western Europe (W. EUR), Southern Europe (S. EUR), the former Czechoslovakia (CZECH), the United States (USA) and a single accession from LIBYA> The accessions and countries of origin are listed in Figure 2.

https://doi.org/10.1371/journal.pone.0022832.g001

PC scores, pertaining to the percent of cotyledons fully emerged after 21 days, also varied dramatically among lines. About 10% of accessions had PC scores >75%; 34% had PC values between 50% and 20%; and ∼10% of accessions that germinated did not advance past initial radicle emergence. PS, survival at 21 days was even more sporadic, with >50% of accessions having survival rates of less than 20%. 32 accessions had no surviving seedlings after 21 days, while only 8 accessions had PS>50%. TD50, time to 50% germination, varied from 2–13 days, with a median of 4 days and a mean of 5.2 days. 85% of accession achieved 50% of total germination within 7 days. Results for 250 mM data were highly correlated with 150 mM data but much sparser and therefore were not used for further analyses.

To explore these phenotypes in the context of genetic relationships and geography, we constructed a UPGMA tree with SNP data and mapped PG onto this tree. Variation in salinity tolerance appears to have a geographic pattern, with Western European and Swedish samples having the highest salinity tolerance and Northern and Southern Europe samples having the lowest salinity tolerance (Figure 2). However, although there may be population structure with respect to variation, most geographic regions maintain considerable variation in salinity tolerance (Figure 2).

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Figure 2. Geographic pattern of salinity tolerance with kinship.

The tree on the right shows a hierarchical clustering (UPGMA) of accessions based on pairwise haplotype sharing. Germination (PG) for each accession is mapped on the left with colors indicating relative values for each accession (red indicates greater % germination, blue less germination). Colors for the accessions labels indicate geographic origin. This tree is generated from the same SNP set as used in Atwell et al. [64], and is essentially identical for accessions shared between datasets.

https://doi.org/10.1371/journal.pone.0022832.g002

Association mapping

The PG, PC and TD50 phenotypes described above were used for association mapping. We did not include PS because the seedling survival data, with only 64 accessions having any surviving seedling, was too sparse for any confidence in association results. For these analyses we used both genome wide SNP markers and SNPs in candidate genes. Before correction for multiple testing, 780 markers were significant (p<0.05). After correction for multiple testing, 46 moderately significant (p<0.05) markers were detected on chromosomes 2, 3, 4 and 5 across all three phenotypes (Figure 3; Table 1).

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Figure 3. Associations between SNP markers and phenotypes.

Peak heights represent negative log p-values from tests of association between genotype and phenotype on chromosomes 2–5. PG, PC and TD50 are plotted in blue circles, black triangles and red squares, respectively. The solid grey line marks a significance level of 0.05%.

https://doi.org/10.1371/journal.pone.0022832.g003

Many of these markers are adjacent and in complete or nearly complete linkage in this set of accessions. In total, association mapping identified ten unique chromosomal regions. Variation in PG was associated with regions on chromosome 2 and chromosome 3. TD50 was associated with both of these regions as well as additional regions on chromosomes 2, 4 and 5. Cotyledon emergence (PC) was associated with the same region TD50 region (but not the PG region) on chromosome 2, the regions associated with germination and TD50 on chromosomes 3 and 5, and two additional regions chromosome 3 (Table 1).

The ten mapped genomic regions contain from 2 to 66 genes per region (data not shown). Three of these regions include obvious gene candidates based on their annotation function (Table 2). These four include AT2G17840, a drought inducible gene that is upregulated in response to salinity stress; AT3G30778, a proline oxidase that is induced by osmotic stress; AT5G12010, an unknown protein that responds to salinity stress, and AT5G38000, an NADP-dependent oxidoreductase involved in response to oxidative stress. In addition to these obvious candidates, these regions include genes involved in ion transport, oxidative stress and several kinases (a class of genes often involved in ionic stress response). For example, the TD50 associated regions on chromosome 2 contains CYCB2 a cyclin-dependent protein kinase involved in cell cycle regulation. Similarly, the region on chromosome 3 contains only two genes, an F-box family protein and a zinc finger family protein involved in zinc ion binding. However the primary association maps to a transposable element that contains a binding site for heat shock J family proteins; members of this heat shock family have been implicated in osmotic stress response [80]. Finally, two adjacent regions on chromosome 5 have a suggestive but non-significant association with both TD50 and with PC (p = 0.081 and p = 0.06). The first of these regions contains several zinc ion binding proteins, the second region contains adjacent NADP-dependent oxidoreductase genes involved in zinc ion binding and up regulated during general plant stress [79].

Quantitative trait analyses

The extensive variation in germination response to salinity suggests that NaCl tolerance in A. thaliana is a polygenic trait. In order to further investigate the genetic basis of this trait we analyzed two different mapping populations for the same set of phenotypes (PG, PC, PS and TD50). The first population was a set of 200 RILs derived from a cross between the Col-4 and Landsberg erecta (Ler-0) accessions [68]. The parental strains had 65% and 28% germination respectively on 150 mM NaCl supplemented media. A second set of 162 RILs derived from a cross of Cvi and Ler, with parental lines having 88% and 24% germination, was also analyzed.

In both populations, there was evidence of significant transgressive segregation between parents for all four phenotypes, because multiple RILS are greater than 2 standard deviations (SDs) more extreme than either parent (Figure 4). Transgressive segregation indicates that both parents likely contain alleles contributing to salinity tolerance. Moreover, phenotypic data for all traits measured was highly correlated between 150 mM NaCl treatment assays and 250 mM treatment assays. As the 250 mM data were quite sparse, we considered only the 150 mM treatment data for QTL mapping in both crosses. Finally, estimated heritabilities under 150 mM salinity conditions for the Cvi×Ler cross were h2 = 0.91 for PG, h2 = 0.71 for PC, h2 = 0.76 TD50, and h2 = 0.57 for PS, (respective heritabilities for the Col×Ler were h2 = 0.67, 0.64, 0.71 and 0.36), indicating that the phenotypes measured are heritable.

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Figure 4. Phenotypic frequencies for the Col × Ler RILs.

Phenotypic means for parental lines are indicated by arrows.

https://doi.org/10.1371/journal.pone.0022832.g004

Col × Ler.

Across the 200 RILS for this cross, mean PG was 66%, mean PC was 45% and mean PS was 25%. TD50 ranged from 2–14 with a mean of 4.5 (Figure 3). There was a significant correlation between PG and PC (r = 0.67; p<0.002), but PS was not significantly correlated with either germination phenotypes (data not shown). TD50 was also not significantly correlated with other phenotypes.

Using a LOD threshold determined by permutation [78], we identified two major QTL for PG on chromosomes 1 (20.9 cM) and 3 (86.4 cM) that together explained over 30% of variation in PG (Table 3). The QTL on chromosome 3 alone explained >22% of PG variance. Lines containing the Columbia allele at this QTL had a mean germination rate of 72% compared to 55% for those with the Ler allele. The QTL on chromosome 1 had a smaller effect; the Ler allele conferred a moderate increase in germination (60% mean germination for the Col allele; 71% mean germination for the Ler allele). The best germinating lines (>80% germination) have the Col allele on chromosome 3 and the Ler allele on chromosome 1, lines with the opposite allelic series have a mean germination rate of 52%. The QTL on chromosome 1 (20.9 cM) is near the SFR5 gene, which is known to be involved in temperature stress response [81], [35]. ABA3, a gene involved in synthesis of the plant stress hormone ABA, is also nearby (25 cM).

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Table 3. QTL for salinity tolerance in Col × Ler and Cvi × Ler crosses, with 95% confidence intervals based on bootstrapping.

https://doi.org/10.1371/journal.pone.0022832.t003

The confidence intervals for QTL contributing to PC overlap two of the QTL for PG. The PC QTL on chromosome 3 maps to a nearly identical location on chromosome 3 (89 cM) (Table 3). However, the other cotyledon QTL maps to a slightly different location on chromosome 1 (28.1 cM) (Figure 5, Table 3), nearer ABA3. Together these QTL in the Col×Ler cross explain ∼26% of PC variation. Similar to PG, the Col allele on chromosome 3 is associated with increased cotyledon emergence, while the Ler allele on chromosome 1 is associated with increased cotyledon emergence.

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Figure 5. QTL positions with LOD scores for all four phenotypes in the Col × Ler cross.

Dashed line indicates the p<0.05 significance threshold, and solid line indicates the p<0.10 significance threshold. Significance thresholds are based on 100,000 permutations for phenotypes.

https://doi.org/10.1371/journal.pone.0022832.g005

Three QTL for time to 50% of total germination (TD50) were found in the Col×Ler cross: a large-effect QTL at 104.1 cM on chromosome 5, a small effect QTL at 12.1 cM on chromosome 5, and an additional small effect QTL on chromosome 3 (39 cM) (Figure 5). Together these three QTL explain ∼22% of variation in TD50 (Table 3). The larger QTL on chromosome 5 is near genes RD29A and RD29B (104.7 cM), which are involved in temperature stress response, drought stress response and confer salt-sensitive knockouts [82, this study, unpublished data]. The other QTL on chromosome 5 is near a member of the Heat Stress Transcription Factor (Hsf) family and is regulated by DREB2A, a transcription factor involved in drought response. The chromosome 3 QTL is not near any known abiotic stress genes. A single small effect QTL for PS was found on chromosome 3 (Table 3). There are no obvious candidate genes at this location but confidence intervals for this QTL overlap those for germination QTL on chromosome 3 (86 cM).

Cvi × Ler.

The Cvi×Ler cross had a mean PG of 72%, but overall the RILs in this cross were less able to survive in the saline environment than the Col×Ler cross, with mean PC 33% (vs. 45%) and mean PS ∼11% (vs. 25%). TD50 ranged from 2–9 days with a mean of 5 days (Figure 3). There was a significant correlation between PG and PC in this cross (r = .71; p<0.003), much like in the Col×Ler cross. PS and TD50 were not significantly correlated with other phenotypes, however (data not shown).

In this cross four QTL for PG were mapped to chromosomes 1, 3, 4 and 5 (Table 3, Figure S1). Together these four QTL explain ∼27% of variation in total germination. The Ler alleles for QTL on chromosomes 1 and 4 contributed to increased PG; the Cvi alleles at the remaining two QTL on chromosomes 3 and 5 (39.0 cM and 77.5 cM) contributed to increased total germination. The QTL on chromosome 1 maps to the same location as that in the Col×Ler cross (19 cM), but is a major QTL in this cross and explains ∼10% of the variation. This QTL is significantly associated with PC as well, with QTL for both traits mapping near the SFR5 gene (cM 19.8). Additional QTL on chromosomes 4 (cM 52.5) and 5 (77.5 cM) were mapped for both PG and PC. The QTL on chromosome 4 (52.5 cM) is not near any known candidate genes, but a scan of loci within this region reveals several candidates (Table 2). The chromosome 5 QTL (77.5) is very near SOS2 (70 cM), which is involved in salinity stress response in the salt overly sensitive pathway. The small effect QTL on chromosome 3 (39.4 cM) is not within the confidence intervals for the QTL mapped on chromosome 3 in the Col×Ler cross. However it also is near ABI3 (38 cM), a gene that is responsive to the plant stress hormone ABA and is active in seeds and required for embryo development [34].

Identifying Candidate Genes with Gene Expression Data

In order to identify potential candidate genes in genomic regions identified by association and QTL mapping, we turned to comparative gene expression data. These data were produced by measuring relative gene expression between control and salt treatments over a time series of seven observations in 36 hours [79]. Out of approximately 24,000 genes on the array, 349 genes were differentially expressed at least 3 fold between control and salinity treatments for at least one time point. We compared these differentially expressed genes to annotated genes from regions identified through both association and QTL analysis. To delimit these regions, we included an arbitrary 1 Mb region around the peak of each inferred QTL, ensuring that we encompassed the 95% confidence regions of all mapped QTL, and 80 kb around the highest scoring association marker. Out of 349 differentially expressed genes, 70 fell within QTL regions, and an additional four lie within association regions (Table S1).

Discussion

In the past five years there has been rapid growth of studies using natural variation in Arabidopsis thaliana to study traits of interest [64], [83]. The motivations for these studies vary from an attempt to exploit natural variation in order to find new genes involved in specific aspects of plant physiology, to trying to understand the molecular basis of adaptations to the local environment. Regardless of the ultimate goal of these studies, the immediate question is usually the same: what is the underlying genetic architecture that affects variation in the trait? This question is unfortunately often difficult to answer. Often even large phenotypic differences are due to variation at multiple loci, and the contribution of any individual locus to the phenotype may be small.

Despite the vast increase in studies using natural variation in Arabidopsis thaliana to study traits of interest, variation in abiotic stress phenotypes has generally not been well studied across a broad group of accessions. One exception is a large-scale association study, which investigated several phenotypes that could be related to abiotic stress, including sodium ion content in plant tissue [64]. However, the current work represents the most comprehensive picture of salinity tolerance variation during germination and early seedling development in Arabidopsis to date. We have shown that extensive variation exists for salinity tolerance in natural accessions of A. thaliana for percent germination (PG), cotyledon emergence (PC), seedling survival (PS) and time to 50% germination (TD50).

Not surprisingly, some of these phenotypes are correlated. For example, we found that PG and PC are highly correlated across our sample of 96 accessions (r2 = 0.69), but PG and PC were less-strongly correlated with PS (r2 = 0.31 and r2 = 0.35). In fact, in some accessions germination was <30% but survival for those seeds that germinated was >90%. These results are in agreement with other studies that suggest salt stress during germination and vegetative growth may have different underlying genetic controls. A similar pattern has been documented in soybean (Glycine max; [84]), wheat (Triticum aestivum; [85]), alfalfa (Medicago sativa; [86]), barley [87], and tomato [88]. TD50 was slightly positively correlated with the germination phenotypes (PG and PC) in both crosses and natural accessions.

Another feature of phenotypic variation for salt tolerance is that it is somewhat geographically structured (Figure 2). However, this variation differs markedly from other phenotypes that have been studied with the same accessions or with other global A. thaliana samples. To the extent we can discriminate with our sample, salt tolerance during germination and seedling growth does not show clinal patterns as have been described in flowering time, drought tolerance, and sodium accumulation phenotypes [50], [51], [89]. A final important feature of phenotypic variation, which was demonstrated previously for one cross [49] and has been repeated here with two, is that phenotypic variance among RILs is larger than either set of parents (Figure 3). This pattern suggests that salinity tolerance in Arabidopsis is polygenic, with alleles from both parental lines contributing to both increases and decreases in salinity tolerance.

We have used both association and QTL approaches to identify genome regions that harbor alleles that contribute to salinity tolerance. QTL mapping remains the most direct approach for identifying causal genes, but it often requires considerable effort and may be quite time consuming to isolate a causative gene. More recently association mapping has enabled mapping variation to smaller genomic regions, but confounding factors such as population structure make it difficult to distinguish true associations from false positives. Correction methods to deal with population structure often eliminate true associations [72]. For these reasons it is often of interest to combine multiple lines of evidence when looking for genetic variation underlying a phenotype of interest [83].

We identified ten genomic regions associated with phenotypic variation in our association mapping panel and 8 larger genomic regions in the QTL mapping. As expected the two crosses generated a largely different set of QTL. The QTL regions included six in the Col×Ler cross and three in the Cvi×Ler cross, with one overlapping region between the crosses. The regions identified by association and QTL analysis overlap in six locations, and thus we have identified 10 genomic regions that are associated with phenotypic variation in salinity tolerance. This suggests there are at least 10 loci involved in salinity tolerance in A. thaliana, which is a similar number to that suggested from QTL studies in barley [87] and in tomato [88]. Many of the regions we have identified are large and may contain multiple loci affecting salinity tolerance. Moreover, it is likely that we have missed many loci due to statistical power; for example, our association results are certainly underpowered with this set of 96 accessions [67], [72]. Residual population structure may also confound true associations with false positives. For both of these reasons, we are unsure of our ability to map any genomic region confidently with the association approach. The QTL approach does not suffer from this lack of power issue, however it is constrained both by the genetic variation contained with the parents used in the crosses and by our limited ability to map effects to small genomic regions.

Nonetheless, we have identified some interesting candidate genes based on location and gene annotation (Table 2). For example, the QTL on chromosome 1 that was found in both crosses (19–20 cM) is near genes ABA3, (24 cM) which encodes a molybdenum cofactor sulfurase involved in the last step of ABA biosynthesis [35], and SFR5 (19.8 cM) a gene involved in freezing tolerance [81]. ABA3 is interesting because it is ABA plays a role in osmotic stress response during germination [90], [91]. It is known that aba and abi mutants can sometimes germinate better then wild type parents during salinity stress [92][93], [49]. Additional evidence for the involvement of ABA response in salinity tolerance comes from studies in barley supporting a role for ABA-related genes in the control of salt responses during germination [87]. This line of evidence suggests that natural allelic variations in genes involved in ABA signaling or biosynthesis are potential candidates to account for the some of the differences in salt responses seen here. However, a whole suite of other environmental stress response genes colocalize with the mapped regions (SFR5, RD26, CBF1, and several DREB family genes; Table 2) might be responsible for the germination differences between strains.

Some of the genes mentioned here as candidates for salinity tolerance are likely to be pleiotropic, affecting multiple different plant phenotypes. For example, there is some evidence from our data that more saline tolerant accessions are also slower to germinate (data not shown). Additionally, because salinity response is physiologically complex, it is likely that salinity tolerance could have similar pleiotropic effects as is seen in drought response, where phenotypes such as development, morphology and growth rate are often affected [94].

We have also examined gene expression data to attempt to verify our candidate genes and our gene regions. Expression studies may provide valuable information by further narrowing the list of candidate genes likely to be causally linked to the trait. Unfortunately, however, no genes have been identified with all three methods – i.e. by association mapping, QTL analyses and gene expression analysis. There is, however, overlap in genes between both mapping approaches and differential expression. Of the 1700 genes in QTL regions and the 215 from our association mapping, 66 overlap but show no evidence of differential expression in saline treatment. However, 71 of the 1700 (4%) genes from the QTL regions are differentially expressed and five of the 215 genes (∼2%) from association mapping overlap with the gene expression data. Of these, only one of our list of ‘strong’ candidates in Table 2 (AT4G19030) is differentially expressed.

There is still considerable debate about the value of comparing differentially expressed genes and mapped genomic locations. There are several issues to consider; first, the genes may not be differentially expressed within a single genotype, but rather between genotypes that differ in salt tolerance, thus we would not see evidence of expression differences within a single accession, as considered in the expression experiment. Second, the expression patterns may differ between tissues; we mapped primarily germination traits, but gene expression was assayed in vegetative tissue. Finally, we may have mapped polymorphic trans-acting regulators of structural genes, and not the genes themselves. If this is true, there is no expectation that mapped regions should coincide with differentially expressed genes. We would expect, however, that further studies of eQTLs would map to the same regions. In other words, although much of the phenotypic variance observed in abiotic stress tolerance is likely due to expression differences, ultimately these expression differences are regulated by genetic changes that can, theoretically, be mapped [95][96]. We expect that as more information about interacting gene networks is available, these analyses will provide additional insight not provided by either mapping or expression analysis singularly [96][97].

Supporting Information

Figure S1.

QTL positions with LOD scores for all four phenotypes in the Cvi×Ler cross. Dashed line indicates the p<0.05 significance threshold, and solid line indicates the p<0.10 significance threshold. Significance thresholds are based on 100,000 permutations for phenotypes.

https://doi.org/10.1371/journal.pone.0022832.s001

(TIF)

Table S1.

Differentially expressed genes that either fall into QTL regions or are associated with salinity response. Genes that lie in a QTL region are listed under EXP_QTL; genes identified by association are listed under EXP_ASSC.

https://doi.org/10.1371/journal.pone.0022832.s002

(XLS)

Acknowledgments

The authors thank J. McKay, S. Lockton, J. Ross-Ibarra and J. Hollister for advice and comments.

Author Contributions

Conceived and designed the experiments: LD-W BSG. Performed the experiments: LD-W. Analyzed the data: LD-W BSG. Contributed reagents/materials/analysis tools: LD-W BSG. Wrote the paper: LD-W BSG.

References

  1. 1. Bohnert HJ, Nelson DE, Jensen RG (1995) Adaptations to environmental stresses. Plant Cell 7(7): 1099–1111.HJ BohnertDE NelsonRG Jensen1995Adaptations to environmental stresses.Plant Cell7710991111
  2. 2. Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Critical Reviews in Plant Sciences 24: 23–58.D. BartelsR. Sunkar2005Drought and salt tolerance in plants.Critical Reviews in Plant Sciences242358
  3. 3. Lowry DB, Hall MC, Salt DE, Willis JH (2009) Genetic and physiological basis of adaptive salt tolerance divergence between coastal and inland Mimulus guttatus. New Phytologist 183: 776–788.DB LowryMC HallDE SaltJH Willis2009Genetic and physiological basis of adaptive salt tolerance divergence between coastal and inland Mimulus guttatus.New Phytologist183776788
  4. 4. Kreps JA, Wu Y, Chang H-S, Zhu T, Wang X, et al. (2002) Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Phys 130: 2129–2141.JA KrepsY. WuH-S ChangT. ZhuX. Wang2002Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress.Plant Phys13021292141
  5. 5. Buchanan CD, Lim S, Salzman RA, Kagiampakis I, Morishige DT, et al. (2005) Sorghum bicolor's Transcriptome Response to Dehydration, High Salinity and ABA. Plant Mol Biol 58: 699–720.CD BuchananS. LimRA SalzmanI. KagiampakisDT Morishige2005Sorghum bicolor's Transcriptome Response to Dehydration, High Salinity and ABA.Plant Mol Biol58699720
  6. 6. Hu X, Zhang A, Zhang J, Jiang M (2006) Abscisic Acid is a key inducer of hydrogen peroxide production in leaves of maize plants exposed to water stress. Plant and Cell Physiology 47: 1484–1495.X. HuA. ZhangJ. ZhangM. Jiang2006Abscisic Acid is a key inducer of hydrogen peroxide production in leaves of maize plants exposed to water stress.Plant and Cell Physiology4714841495
  7. 7. Teixeira J, Pereira S (2007) High salinity and drought act on an organ-dependent manner on potato glutamine synthetase expression and accumulation. Environmental and Experimental Botany 60: 121–126.J. TeixeiraS. Pereira2007High salinity and drought act on an organ-dependent manner on potato glutamine synthetase expression and accumulation.Environmental and Experimental Botany60121126
  8. 8. Hassine AB, Ghanem ME, Bouzid S, Lutts S (2008) An inland and a coastal population of the Mediterranean xero-halophyte species Atriplex halimus L. differ in their ability to accumulate proline and glycinebetaine in response to salinity and water stress. Journal of Experimental Botany 59: 1315–1326.AB HassineME GhanemS. BouzidS. Lutts2008An inland and a coastal population of the Mediterranean xero-halophyte species Atriplex halimus L. differ in their ability to accumulate proline and glycinebetaine in response to salinity and water stress.Journal of Experimental Botany5913151326
  9. 9. Johnson DA, Asay KH, Read JJ (1993) Genotypic and Environmental Variation for Carbon Isotope Discrimination in Crested Wheatgrass, A Perennial Forage Grass. In: Ehleringer JR, Hall AE, Farquhar GD, editors. Stable isotopes and plant carbon-water relations. San Diego: Academic Press. pp. 269–280.DA JohnsonKH AsayJJ Read1993Genotypic and Environmental Variation for Carbon Isotope Discrimination in Crested Wheatgrass, A Perennial Forage Grass.JR EhleringerAE HallGD FarquharStable isotopes and plant carbon-water relationsSan DiegoAcademic Press269280
  10. 10. Heschel MS, Sultan SE, Glover S, Sloan D (2004) Population differentiation and plastic responses to drought stress in the generalist annual, Polygonum persicaria. International Journal of Plant Sciences 165: 817–824.MS HeschelSE SultanS. GloverD. Sloan2004Population differentiation and plastic responses to drought stress in the generalist annual, Polygonum persicaria.International Journal of Plant Sciences165817824
  11. 11. Easlon HM, Richards JH (2009) Drought Response in self-compatible species of tomato (Solanaceae). American Journal of Botany 96: 605–611.HM EaslonJH Richards2009Drought Response in self-compatible species of tomato (Solanaceae).American Journal of Botany96605611
  12. 12. Sherrard ME, Maherali H, Latta RG (2009) Water stress alters the genetic architecture of functional traits associated with drought adaptation in Avena barbata. Evolution 63: 702–715.ME SherrardH. MaheraliRG Latta2009Water stress alters the genetic architecture of functional traits associated with drought adaptation in Avena barbata.Evolution63702715
  13. 13. Flowers TJ, Yeo AR (1995) Breeding for salinity resistance in crop plants: Where next? Australian Journal of Plant Physiology 22(6): 875–884.TJ FlowersAR Yeo1995Breeding for salinity resistance in crop plants: Where next?Australian Journal of Plant Physiology226875884
  14. 14. Nelson D, Shen B, Bohnert HJ (1998) Salinity tolerance: mechanisms, models and the metabolic engineering of complex traits. Genet Eng (NY) 20: 153–176.D. NelsonB. ShenHJ Bohnert1998Salinity tolerance: mechanisms, models and the metabolic engineering of complex traits.Genet Eng (NY)20153176
  15. 15. Zhu J-K (2003) Regulation of ion homeostasis under salt stress. Current Opinion in Plant Biology 6(5): 441–445.J-K Zhu2003Regulation of ion homeostasis under salt stress.Current Opinion in Plant Biology65441445
  16. 16. Zhu J-K (2001) Plant salt tolerance. Trends in Plant Science 6(2): 66–71.J-K Zhu2001Plant salt tolerance.Trends in Plant Science626671
  17. 17. Bianchi G, Gamba A, Murelli C, Salamini F, Bartels D (1991) Novel carbohydrate metabolism in the resurrection plant Craterostigma plantagineum. Plant Journal 1: 335–359.G. BianchiA. GambaC. MurelliF. SalaminiD. Bartels1991Novel carbohydrate metabolism in the resurrection plant Craterostigma plantagineum.Plant Journal1335359
  18. 18. Ishitani M, Nakamura T, Han SY, Takabe T (1995) Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid. Plant Molecular Biology 27: 307–315.M. IshitaniT. NakamuraSY HanT. Takabe1995Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid.Plant Molecular Biology27307315
  19. 19. Yoshiba Y, Kiyosue T, Katagiri T, Ueda H, Mizoguchi T, et al. (1995) Correlation between the induction of a gene for 1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress. The Plant Journal 7: 751–760.Y. YoshibaT. KiyosueT. KatagiriH. UedaT. Mizoguchi1995Correlation between the induction of a gene for 1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress.The Plant Journal7751760
  20. 20. McCue KF, Hanson AD (1992) Salt-Inducible Betaine Aldehyde Dehydrogenase from Sugar-Beet - cDNA Cloning and Expression. Plant Molecular Biology 18(1): 1–11.KF McCueAD Hanson1992Salt-Inducible Betaine Aldehyde Dehydrogenase from Sugar-Beet - cDNA Cloning and Expression.Plant Molecular Biology181111
  21. 21. Ingram J, Bartels D (2003) The molecular basis of dehydration tolerance in plants. Annual Review of Plant Physiology and Plant Molecular Biology 47(1): 377–403.J. IngramD. Bartels2003The molecular basis of dehydration tolerance in plants.Annual Review of Plant Physiology and Plant Molecular Biology471377403
  22. 22. Hoekstra FA, Golovina EA, Buitink J (2001) Mechanisms of plant desiccation tolerance. Trends in Plant Science 6(9): 431–438.FA HoekstraEA GolovinaJ. Buitink2001Mechanisms of plant desiccation tolerance.Trends in Plant Science69431438
  23. 23. Flowers TJ, Troke PF, Yeo AR (1977) Mechanism of Salt Tolerance in Halophytes. Annual Review of Plant Physiology and Plant Molecular Biology 28: 89–121.TJ FlowersPF TrokeAR Yeo1977Mechanism of Salt Tolerance in Halophytes.Annual Review of Plant Physiology and Plant Molecular Biology2889121
  24. 24. Greenway H, Munns R (1980) Mechanism of salt tolerance in non-halophytes. Annual Review of Plant Physiology and Plant Molecular Biology 31: 149–190.H. GreenwayR. Munns1980Mechanism of salt tolerance in non-halophytes.Annual Review of Plant Physiology and Plant Molecular Biology31149190
  25. 25. Ehret DL, Plant AL (1999) Salt tolerance in crop plants. In: Dhaliwal GS, Arora R, editors. Environmental Stress in Crop Plants. New Delhi: Commonwealth Publishers. pp. 69–120.DL EhretAL Plant1999Salt tolerance in crop plants.GS DhaliwalR. AroraEnvironmental Stress in Crop PlantsNew DelhiCommonwealth Publishers69120
  26. 26. Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2003) Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology 51(1): 463–499.PM HasegawaRA BressanJK ZhuHJ Bohnert2003Plant cellular and molecular responses to high salinity.Annual Review of Plant Physiology and Plant Molecular Biology511463499
  27. 27. Seki M, Kamei A, Yamaguchi-Shinozaki K, Shinozaki K (2003) Molecular responses to drought, salinity and frost: common and different paths for plant protection. Current Opinion in Biotechnology 14: 194–199.M. SekiA. KameiK. Yamaguchi-ShinozakiK. Shinozaki2003Molecular responses to drought, salinity and frost: common and different paths for plant protection.Current Opinion in Biotechnology14194199
  28. 28. Wang W, Vinocur B, Altman A (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218: 1–14.W. WangB. VinocurA. Altman2003Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance.Planta218114
  29. 29. Zhu J-K (2003) Salt and drought stress signal transduction in plants. Annual Review of Plant Biology 53: 247–273.J-K Zhu2003Salt and drought stress signal transduction in plants.Annual Review of Plant Biology53247273
  30. 30. Zhang H-X, Blumwald E (2001) Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nat Biotech 19: 765–768.H-X ZhangE. Blumwald2001Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit.Nat Biotech19765768
  31. 31. Zhang H-X, Hodson J, Williams JP, Blumwald E (2001) Engineering salt-tolerant Brassica plants: Characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation. Proc Nat Acad Sci USA 98: 12832–12836.H-X ZhangJ. HodsonJP WilliamsE. Blumwald2001Engineering salt-tolerant Brassica plants: Characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation.Proc Nat Acad Sci USA981283212836
  32. 32. Qiu QS, Guo Y, Dietrich MA, Schumaker KS, Zhu J-K (2002) Regulation of SOS1, a plasma membrane Na+/H+ exchanger in Arabidopsis thaliana, by SOS2 and SOS3. Proc Natl Acad Sci USA 99: 8436–41.QS QiuY. GuoMA DietrichKS SchumakerJ-K Zhu2002Regulation of SOS1, a plasma membrane Na+/H+ exchanger in Arabidopsis thaliana, by SOS2 and SOS3.Proc Natl Acad Sci USA99843641
  33. 33. Liu J, Ishitani M, Halfter U, Kim C-S, Zhu JK (2000) The Arabidopsis thaliana SOS2 gene encodes a protein kinase that is required for salt tolerance. PNAS 97: 3730–3734.J. LiuM. IshitaniU. HalfterC-S KimJK Zhu2000The Arabidopsis thaliana SOS2 gene encodes a protein kinase that is required for salt tolerance.PNAS9737303734
  34. 34. Giraudat J, Hauge BM, Valon C, Smalle J, Parcy F, Goodman HM (1992) Isolation of the Arabidopsis ABI3 gene by positional cloning. The Plant Cell 4: 1251–1261.J. GiraudatBM HaugeC. ValonJ. SmalleF. ParcyHM Goodman1992Isolation of the Arabidopsis ABI3 gene by positional cloning.The Plant Cell412511261
  35. 35. Xiong L, Ishitani M, Lee H, Zhu J-K (2001) The Arabidopsis LOS5/ABA3 locus encodes a molybdenum cofactor sulfurase and modulates cold stress- and osmotic stress-responsive gene expression. The Plant Cell 13: 2063–2083.L. XiongM. IshitaniH. LeeJ-K Zhu2001The Arabidopsis LOS5/ABA3 locus encodes a molybdenum cofactor sulfurase and modulates cold stress- and osmotic stress-responsive gene expression.The Plant Cell1320632083
  36. 36. Ren Z, Zheng Z, Chinnusamy V, Zhu J, Cui X, et al. (2010) RAS1, a quantitative trait locus for salt tolerance and ABA sensitivity in Arabidopsis. Proc Natl Acad Sci USA 2010 107: 5669–5674.Z. RenZ. ZhengV. ChinnusamyJ. ZhuX. Cui2010RAS1, a quantitative trait locus for salt tolerance and ABA sensitivity in Arabidopsis.Proc Natl Acad Sci USA 201010756695674
  37. 37. Horvath DP, McLarney BK, Thomashow MF (1993) Regulation of Arabidopsis thaliana cor78 in response to low temperature. Plant Physiology 103: 1047–1053.DP HorvathBK McLarneyMF Thomashow1993Regulation of Arabidopsis thaliana cor78 in response to low temperature.Plant Physiology10310471053
  38. 38. Imai R, Chang L, Ohta A, Bray E, Takagi M (1996) A lea-class gene of tomato confers salt and freezing tolerance when expressed in Saccharomyces cerevisiae. Gene 170: 243–248.R. ImaiL. ChangA. OhtaE. BrayM. Takagi1996A lea-class gene of tomato confers salt and freezing tolerance when expressed in Saccharomyces cerevisiae.Gene170243248
  39. 39. Xu D, Duan X, Wang B, Hong B, Ho THD, et al. (1996) Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice. Plant Physiology 110: 249–257.D. XuX. DuanB. WangB. HongTHD Ho1996Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice.Plant Physiology110249257
  40. 40. Taji T, Seki M, Yamaguchi-Shinozaki K, Kamada H, Giraudat J, Shinozaki K (1999) Mapping of 25 Drought-Inducible Genes, RD and ERD, in Arabidopsis thaliana. Plant and Cell Physiology 40: 119–123.T. TajiM. SekiK. Yamaguchi-ShinozakiH. KamadaJ. GiraudatK. Shinozaki1999Mapping of 25 Drought-Inducible Genes, RD and ERD, in Arabidopsis thaliana.Plant and Cell Physiology40119123
  41. 41. Hong Z, Lakkineni K, Zhang Z, Verma DPS (2000) Removal of feedback inhibition of Delta 1-Pyrroline-5-Carboxylate Synthetase results in increased Proline accumulation and protection of plants from osmotic stress. Plant Physiology 122: 1129–1136.Z. HongK. LakkineniZ. ZhangDPS Verma2000Removal of feedback inhibition of Delta 1-Pyrroline-5-Carboxylate Synthetase results in increased Proline accumulation and protection of plants from osmotic stress.Plant Physiology12211291136
  42. 42. Sreenivasulu N, Sopory SK, Kavi Kishor PB (2007) Deciphering the regulatory mechanisms of abiotic stress tolerance in plants by genomic approaches. Gene 388: 1–13.N. SreenivasuluSK SoporyPB Kavi Kishor2007Deciphering the regulatory mechanisms of abiotic stress tolerance in plants by genomic approaches.Gene388113
  43. 43. Umezawa T, Okamoto M, Kushiro T, Nambara E, Oono Y, et al. (2006) CYP707A3, a major ABA 8′-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana. Plant J 46: 171–182.T. UmezawaM. OkamotoT. KushiroE. NambaraY. Oono2006CYP707A3, a major ABA 8′-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana.Plant J46171182
  44. 44. Zeller G, Henz S, Widmer CK, Sachsenberg T, Ratsch G, et al. (2009) Stress-induced changes in the Arabidopsis thaliana transcriptome analyzed using whole-genome tiling arrays. The Plant Journal 58: 1068–1082.G. ZellerS. HenzCK WidmerT. SachsenbergG. Ratsch2009Stress-induced changes in the Arabidopsis thaliana transcriptome analyzed using whole-genome tiling arrays.The Plant Journal5810681082
  45. 45. Hugovieux V, Kwak JM, Schroeder JI (2001) An mRNA cap binding protein, ABH1, modulates early abscisic acid signal transduction in Arabidopsis. Cell 106: 477–487.V. HugovieuxJM KwakJI Schroeder2001An mRNA cap binding protein, ABH1, modulates early abscisic acid signal transduction in Arabidopsis.Cell106477487
  46. 46. Zhu J-K (2000) Genetic analysis of plant salt tolerance using Arabidopsis. Plant Physiology 124: 941–948.J-K Zhu2000Genetic analysis of plant salt tolerance using Arabidopsis.Plant Physiology124941948
  47. 47. Masle J, Gilmore SR, Farquhar GD (2005) The ERECTA gene regulates plant transpiration efficiency in Arabidopsis. Nature 436: 866–870.J. MasleSR GilmoreGD Farquhar2005The ERECTA gene regulates plant transpiration efficiency in Arabidopsis.Nature436866870
  48. 48. Nienhuis J, Sills GR, Martin B, King G (1994) Variance for Water-Use Efficiency among ecotypes and Recombinant Inbred Lines of Arabidopsis thaliana (Brassicaceae). American Journal of Botany 81: 943–947.J. NienhuisGR SillsB. MartinG. King1994Variance for Water-Use Efficiency among ecotypes and Recombinant Inbred Lines of Arabidopsis thaliana (Brassicaceae).American Journal of Botany81943947
  49. 49. Quesada V, Ponce MR, Micol JL (2003) Genetic analysis of salt-tolerant mutants in Arabidopsis thaliana. Genetics 154: 421–436.V. QuesadaMR PonceJL Micol2003Genetic analysis of salt-tolerant mutants in Arabidopsis thaliana.Genetics154421436
  50. 50. McKay JK, Richards JH, Mitchell-Olds T (2003) Genetics of drought adaptation in Arabidopsis thaliana: Pleiotropy contributes to genetic correlations among ecological traits. Molecular Ecology 12: 1137–1151.JK McKayJH RichardsT. Mitchell-Olds2003Genetics of drought adaptation in Arabidopsis thaliana: Pleiotropy contributes to genetic correlations among ecological traits.Molecular Ecology1211371151
  51. 51. Baxter I, Brazelton JN, Yu D, Huang YS, Lahner B, et al. (2010) A Coastal cline in sodium accumulation in Arabidopsis thaliana in driven by natural variation of the sodium transporter AtHKT1;1. Plos Genetics 6(11): e1001193.I. BaxterJN BrazeltonD. YuYS HuangB. Lahner2010A Coastal cline in sodium accumulation in Arabidopsis thaliana in driven by natural variation of the sodium transporter AtHKT1;1.Plos Genetics611e1001193
  52. 52. Geber MA, Griffen LR (2003) Inheritance and natural selection on functional traits. Int J Plant Sci 164: S21–S42.MA GeberLR Griffen2003Inheritance and natural selection on functional traits. IntJ Plant Sci164S21S42
  53. 53. Culley TM, Dunbar-Wallis AK, Sakai AK, Weller SG, et al. (2006) Genetic variation of ecophysiological traits in two gynodioecious species of Schiedea (Caryophyllaceae). New Phytologist 169: 589–601.TM CulleyAK Dunbar-WallisAK SakaiSG Weller2006Genetic variation of ecophysiological traits in two gynodioecious species of Schiedea (Caryophyllaceae).New Phytologist169589601
  54. 54. Lexer C, Welch ME, Durphy JL, Rieseberg LH (2003) Natural selection for salt tolerance quantitative trait loci (QTLs) in wild sunflower hybrids: Implications for the origin of Helianthus paradoxus, a diploid hybrid species. Molecular Ecology 12: 1225–1235.C. LexerME WelchJL DurphyLH Rieseberg2003Natural selection for salt tolerance quantitative trait loci (QTLs) in wild sunflower hybrids: Implications for the origin of Helianthus paradoxus, a diploid hybrid species.Molecular Ecology1212251235
  55. 55. Hausmann N, Juenger T, Sen S, Stowe KA, Dawson TE, et al. (2005) Quantitative trait loci affecting delta13C and response to differential water availibility in Arabidopsis thaliana. Evolution 59: 81–96.N. HausmannT. JuengerS. SenKA StoweTE Dawson2005Quantitative trait loci affecting delta13C and response to differential water availibility in Arabidopsis thaliana.Evolution598196
  56. 56. Juenger T, Sen S, Stowe KA, Simms EL (2005) Epistasis and genotype-environment interaction for quantitative trait loci affecting flowering time in Arabidopsis thaliana. Genetica 123: 87–105.T. JuengerS. SenKA StoweEL Simms2005Epistasis and genotype-environment interaction for quantitative trait loci affecting flowering time in Arabidopsis thaliana.Genetica12387105
  57. 57. Alonso-Blanco C, Gomez-Mena C, Llorente F, Koornneef M, Salinas J, Martínez-Zapater JM (2005) Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance Quantitative Trait Locus in Arabidopsis. Plant Physiology 139: 304–1312.C. Alonso-BlancoC. Gomez-MenaF. LlorenteM. KoornneefJ. SalinasJM Martínez-Zapater2005Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance Quantitative Trait Locus in Arabidopsis.Plant Physiology1393041312
  58. 58. Hannah MA, Wiese D, Freund S, Fiehn O, Heyer AG, Hincha DK (2006) Natural Genetic Variation of Freezing Tolerance in Arabidopsis. Plant Physiology 142(1): 98–112.MA HannahD. WieseS. FreundO. FiehnAG HeyerDK Hincha2006Natural Genetic Variation of Freezing Tolerance in Arabidopsis.Plant Physiology142198112
  59. 59. Zhen Y, Ungerer MC (2008) Relaxed selection on the CBF/DREB1 regulatory genes and reduced freezing tolerance in the southern range of Arabidopsis thaliana. Molecular Biology and Evolution 25: 2547–2555.Y. ZhenMC Ungerer2008Relaxed selection on the CBF/DREB1 regulatory genes and reduced freezing tolerance in the southern range of Arabidopsis thaliana.Molecular Biology and Evolution2525472555
  60. 60. Courbot M, Willems G, Motte P, Arvidsson S, Roosens N, et al. (2007) A Major Quantitative Trait Locus for cadmium tolerance in Arabidopsis halleri colocalizes with HMA4, a gene encoding a heavy metal ATPase. Plant Physiology 144: 1052–1065.M. CourbotG. WillemsP. MotteS. ArvidssonN. Roosens2007A Major Quantitative Trait Locus for cadmium tolerance in Arabidopsis halleri colocalizes with HMA4, a gene encoding a heavy metal ATPase.Plant Physiology14410521065
  61. 61. Brachi B, Faure N, Horton M, Flahauw E, Vazquez A, et al. (2010) Linkage and Association Mapping of Arabidopsis thaliana Flowering Time in Nature. PLoS Genet 6: e1000940.B. BrachiN. FaureM. HortonE. FlahauwA. Vazquez2010Linkage and Association Mapping of Arabidopsis thaliana Flowering Time in Nature.PLoS Genet6e1000940
  62. 62. Kim JK, Bamba T, Harada K, Fukusaki E, Kobayashi A (2007) Time-course metabolic profiling in Arabidopsis thaliana cell cultures after salt stress treatment. Journal of Experimental Botany 58(3): 415–424.JK KimT. BambaK. HaradaE. FukusakiA. Kobayashi2007Time-course metabolic profiling in Arabidopsis thaliana cell cultures after salt stress treatment.Journal of Experimental Botany583415424
  63. 63. Nordborg M, Weigel D (2008) Next-generation genetics in plants. Nature 456(7223): 720–723.M. NordborgD. Weigel2008Next-generation genetics in plants.Nature4567223720723
  64. 64. Atwell S, Huang YS, Vilhjalmsson BJ, Willems G, Horton M, et al. (2010) Genome-wide association study of 107 phenotypes in Arabidopsis thaliana inbred lines. Nature 465: 627–631.S. AtwellYS HuangBJ VilhjalmssonG. WillemsM. Horton2010Genome-wide association study of 107 phenotypes in Arabidopsis thaliana inbred lines.Nature465627631
  65. 65. Katori T, Ikeda A, Iuchi S, Kobayashi M, Shinozaki K, et al. (2010) Dissecting the genetic control of natural variation in salt tolerance of Arabidopsis thaliana accessions. J Exp Botany 61: 1125–1138.T. KatoriA. IkedaS. IuchiM. KobayashiK. Shinozaki2010Dissecting the genetic control of natural variation in salt tolerance of Arabidopsis thaliana accessions.J Exp Botany6111251138
  66. 66. Nordborg M, Hu TT, Ishino Y, Jhaveri J, Toomajian C, et al. (2005) The Pattern of Polymorphism in Arabidopsis thaliana. PLoS Biology 3: e196.M. NordborgTT HuY. IshinoJ. JhaveriC. Toomajian2005The Pattern of Polymorphism in Arabidopsis thaliana.PLoS Biology3e196
  67. 67. Aranzana MJ, Kim S, Zhao K, Bakker E, Horton M, et al. (2005) Genome-Wide Association Mapping in Arabidopsis Identifies Previously Known Flowering Time and Pathogen Resistance Genes. PLoS Genetics 1: e60.MJ AranzanaS. KimK. ZhaoE. BakkerM. Horton2005Genome-Wide Association Mapping in Arabidopsis Identifies Previously Known Flowering Time and Pathogen Resistance Genes.PLoS Genetics1e60
  68. 68. Lister C, Dean C (1993) Recombinant inbred lines for mapping RFLP and phenotypic markers in Arabidopsis thaliana. Plant Journal 4: 745–750.C. ListerC. Dean1993Recombinant inbred lines for mapping RFLP and phenotypic markers in Arabidopsis thaliana.Plant Journal4745750
  69. 69. Alonso-Blanco C, Peeters AJ, Koorneef M, Lister C, Dean C, et al. (1998) Development of an AFLP based linkage map of Ler, Col and Cvi Arabidopsis thaliana ecotypes and construction of a Ler/Cvi recombinant inbred line population. The Plant Journal 14: 259–271.C. Alonso-BlancoAJ PeetersM. KoorneefC. ListerC. Dean1998Development of an AFLP based linkage map of Ler, Col and Cvi Arabidopsis thaliana ecotypes and construction of a Ler/Cvi recombinant inbred line population.The Plant Journal14259271
  70. 70. Becker RA, Chambers JM, Wilks AR (1988) The New S Language. New York: Wadsworth & Brooks/Cole. pp. 451–459.RA BeckerJM ChambersAR Wilks1988The New S LanguageNew YorkWadsworth & Brooks/Cole451459
  71. 71. Yu J, Pressoir G, Briggs W, Vroh Bi I, Yamasaki M, et al. (2006) A unified mixed-model method for association mapping that accounts for multiple levels of relatedness. Nature Genetics 38: 203–208.J. YuG. PressoirW. BriggsI. Vroh BiM. Yamasaki2006A unified mixed-model method for association mapping that accounts for multiple levels of relatedness.Nature Genetics38203208
  72. 72. Zhao K, Aranzana MJ, Kim S, Lister C, Shindo C, et al. (2007) An Arabidopsis example of Association Mapping in structured samples. PLoS Genet 3: e4.K. ZhaoMJ AranzanaS. KimC. ListerC. Shindo2007An Arabidopsis example of Association Mapping in structured samples.PLoS Genet3e4
  73. 73. Price AL, Patterson NJ, Plenge RM, Weinblatt ME, Shadick NA, et al. (2006) Principal components analysis corrects for stratification in genome-wide association studies. Nat Genet 38: 904–909.AL PriceNJ PattersonRM PlengeME WeinblattNA Shadick2006Principal components analysis corrects for stratification in genome-wide association studies.Nat Genet38904909
  74. 74. Bradbury PJ, Zhang Z, Kroon DE, Casstevens TM, Ramdoss Y, et al. (2007) TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics 23: 2633–2635.PJ BradburyZ. ZhangDE KroonTM CasstevensY. Ramdoss2007TASSEL: software for association mapping of complex traits in diverse samples.Bioinformatics2326332635
  75. 75. Broman KW, Wu H, Sen S, Churchill GA (2003) R/qtl: QTL mapping in experimental crosses. Bioinformatics 19: 889–890.KW BromanH. WuS. SenGA Churchill2003R/qtl: QTL mapping in experimental crosses.Bioinformatics19889890
  76. 76. Peters JL, Constandt H, Neyt P, Cnops G, Zethof J, et al. (2001) A physical Amplified Fragment-Length Polymorphism map of Arabidopsis. Plant Physiology 127: 1579–1589.JL PetersH. ConstandtP. NeytG. CnopsJ. Zethof2001A physical Amplified Fragment-Length Polymorphism map of Arabidopsis.Plant Physiology12715791589
  77. 77. Churchill GA, Doerge RW (1994) Empirical Threshold Values for Quantitative Trait Mapping. Genetics 138(3): 963–971.GA ChurchillRW Doerge1994Empirical Threshold Values for Quantitative Trait Mapping.Genetics1383963971
  78. 78. Doerge RW, Churchill GA (1996) Permutation tests for multiple loci affecting a Quantitative Character. Genetics 142: 285–294.RW DoergeGA Churchill1996Permutation tests for multiple loci affecting a Quantitative Character.Genetics142285294
  79. 79. Kilian J, Whitehead D, Horak J, Wanke D, Weinl S, et al. (2007) The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses. The Plant Journal 50: 347–363.J. KilianD. WhiteheadJ. HorakD. WankeS. Weinl2007The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses.The Plant Journal50347363
  80. 80. Volker U, Mach H, Schmid R, Hecker M (1992) Stress proteins and cross-protection by heat shock and salt stress in Bacillus subtilis. Journal of General Microbiology 138: 2125–2135.U. VolkerH. MachR. SchmidM. Hecker1992Stress proteins and cross-protection by heat shock and salt stress in Bacillus subtilis.Journal of General Microbiology13821252135
  81. 81. Thorlby G, Fourrier N, Warren G (2004) The SENSITIVE TO FREEZING2 Gene, Required for Freezing Tolerance in Arabidopsis thaliana, Encodes a {beta}-Glucosidase. The Plant Cell 16: 2192–2203.G. ThorlbyN. FourrierG. Warren2004The SENSITIVE TO FREEZING2 Gene, Required for Freezing Tolerance in Arabidopsis thaliana, Encodes a {beta}-Glucosidase.The Plant Cell1621922203
  82. 82. Nordin K, Vahala T, Palva ET (1993) Differential expression of two related, low-temperature-induced genes in Arabidopsis thaliana. Plant Molecular Biology 21: 641–653.K. NordinT. VahalaET Palva1993Differential expression of two related, low-temperature-induced genes in Arabidopsis thaliana.Plant Molecular Biology21641653
  83. 83. Weigel D, Nordborg M (2005) Natural Variation in Arabidopsis. How do we find the causal genes? Plant Physiology 138: 567–568.D. WeigelM. Nordborg2005Natural Variation in Arabidopsis. How do we find the causal genes?Plant Physiology138567568
  84. 84. Abel G, Mackenzie A (1963) Salt tolerance of soybean varieties (Glycine max L. Merrill) during germination and later growth. Crop Science 3: 159–161.G. AbelA. Mackenzie1963Salt tolerance of soybean varieties (Glycine max L. Merrill) during germination and later growth.Crop Science3159161
  85. 85. Kumar D, Singh B, Singh RK (1983) Salt tolerance in wheat varieties. SABRAO J 15: 71–76.D. KumarB. SinghRK Singh1983Salt tolerance in wheat varieties.SABRAO J157176
  86. 86. Johnson DW, Smith SE, Dobrenz AK (1992) Genetic and phenotypic relationships in response to NaCl at different developmental stages in alfalfa. Theoretical Applied Genetics 83: 833–838.DW JohnsonSE SmithAK Dobrenz1992Genetic and phenotypic relationships in response to NaCl at different developmental stages in alfalfa.Theoretical Applied Genetics83833838
  87. 87. Mano Y, Takeda K (1997) Mapping quantitative trait loci for salt tolerance at germination and the seedling stage in barley (Hordeum vulgare L.). Euphytica 94(3): 263–272.Y. ManoK. Takeda1997Mapping quantitative trait loci for salt tolerance at germination and the seedling stage in barley (Hordeum vulgare L.).Euphytica943263272
  88. 88. Foolad MR, Hyman JR, Lin GY (1999) Relationships between cold- and salt-tolerance during seed germination in tomato: Analysis of response and correlated response to selection. Plant Breeding 118: 49–52.MR FooladJR HymanGY Lin1999Relationships between cold- and salt-tolerance during seed germination in tomato: Analysis of response and correlated response to selection.Plant Breeding1184952
  89. 89. Stinchcombe JR, Weinig C, Ungerer M, Olsen KM, Mays C, et al. (2004) A latitudinal cline in flowering time in Arabidopsis thaliana modulated by the flowering time gene FRIGIDA. Proc Natl Acad Sci USA 101(13): 4712–4717.JR StinchcombeC. WeinigM. UngererKM OlsenC. Mays2004A latitudinal cline in flowering time in Arabidopsis thaliana modulated by the flowering time gene FRIGIDA.Proc Natl Acad Sci USA1011347124717
  90. 90. Begum F, Karmoker JL, Fattah QA, Maniruzzaman AFM (1992) The effect of salinity on germination and its correlation with K+, Na+, Cl− accumulation in germinating seeds of Triticum aestivum L. cv. Akbar. Plant and Cell Physiology 33: 1009–1014.F. BegumJL KarmokerQA FattahAFM Maniruzzaman1992The effect of salinity on germination and its correlation with K+, Na+, Cl− accumulation in germinating seeds of Triticum aestivum L. cv. Akbar.Plant and Cell Physiology3310091014
  91. 91. Ni BR, Bradford KJ (1993) Germination and Dormancy of Abscisic Acid- and Gibberellin-Deficient Mutant Tomato (Lycopersicon esculentum) Seeds (Sensitivity of Germination to Abscisic Acid, Gibberellin, and Water Potential). Plant Physiology 101: 607–617.BR NiKJ Bradford1993Germination and Dormancy of Abscisic Acid- and Gibberellin-Deficient Mutant Tomato (Lycopersicon esculentum) Seeds (Sensitivity of Germination to Abscisic Acid, Gibberellin, and Water Potential).Plant Physiology101607617
  92. 92. Werner JE, Finkelstein RR (1995) Arabidopsis mutants with reduced response to NaCl and osmotic stress. Physiol Plant 93: 659–666.JE WernerRR Finkelstein1995Arabidopsis mutants with reduced response to NaCl and osmotic stress. PhysiolPlant93659666
  93. 93. Leon-Kloosterziel KM, Gil MA, Ruijs GJ, Jacobsen SE, Olszewski NE, et al. (1996) Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci. Plant Journal 10: 655–661.KM Leon-KloosterzielMA GilGJ RuijsSE JacobsenNE Olszewski1996Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci.Plant Journal10655661
  94. 94. Juenger TE, Wayne T, Boles S, Symonds VV, McKay J, et al. (2006) Natural genetic variation in whole-genome expression in Arabidopsis thaliana: the impact of physiological QTL introgression. Molecular Ecology 15: 1351–1365.TE JuengerT. WayneS. BolesVV SymondsJ. McKay2006Natural genetic variation in whole-genome expression in Arabidopsis thaliana: the impact of physiological QTL introgression.Molecular Ecology1513511365
  95. 95. Farrall M (2004) Quantitative genetic variation: a post-modern view. Human Molecular Genetics 13: R1–R7.M. Farrall2004Quantitative genetic variation: a post-modern view.Human Molecular Genetics13R1R7
  96. 96. Gilad Y, Rifkin SA, Pritchard JK (2008) Revealing the architecture of gene regulation: the promise of eQTL studies. Trends in Genetics 24: 408–415.Y. GiladSA RifkinJK Pritchard2008Revealing the architecture of gene regulation: the promise of eQTL studies.Trends in Genetics24408415
  97. 97. Kliebenstein D (2009) Quantitative Genomics: Analyzing Intraspecific Variation Using Global Gene Expression Polymorphisms or eQTLs. Annual Review of Plant Biology 60: 93–114.D. Kliebenstein2009Quantitative Genomics: Analyzing Intraspecific Variation Using Global Gene Expression Polymorphisms or eQTLs.Annual Review of Plant Biology6093114
  98. 98. Thorlby G, Veale E, Butcher K, Warren G (1999) Map positions of SFR genes in relation to other freezing-related genes of Arabidopsis thaliana. Plant J 17: 445–452.G. ThorlbyE. VealeK. ButcherG. Warren1999Map positions of SFR genes in relation to other freezing-related genes of Arabidopsis thaliana.Plant J17445452
  99. 99. Lee BH, Kapoor A, Zhu JH, Zhu JK (2006) STABILIZED1, a stress-upregulated nuclear protein, is required for pre-mRNA splicing, mRNA turnover, and stress tolerance in Arabidopsis. Plant Cell 18: 1736–1749.BH LeeA. KapoorJH ZhuJK Zhu2006STABILIZED1, a stress-upregulated nuclear protein, is required for pre-mRNA splicing, mRNA turnover, and stress tolerance in Arabidopsis.Plant Cell1817361749
  100. 100. Fujii H, Zhu JK (2009) An Autophosphorylation Site of the Protein Kinase SOS2 Is Important for Salt Tolerance in Arabidopsis. Mol Plant 2: 183–190.H. FujiiJK Zhu2009An Autophosphorylation Site of the Protein Kinase SOS2 Is Important for Salt Tolerance in Arabidopsis.Mol Plant2183190
  101. 101. Barrero JM, Rodriguez PL, Quesada V, Piqueras P, Ponce MR, et al. (2006) Both abscisic acid (ABA)-dependent and ABA-independent pathways govern the induction of NCED3, AAO3 and ABA1 in response to salt stress. Plant Cell Environ 29: 2000–2008.JM BarreroPL RodriguezV. QuesadaP. PiquerasMR Ponce2006Both abscisic acid (ABA)-dependent and ABA-independent pathways govern the induction of NCED3, AAO3 and ABA1 in response to salt stress.Plant Cell Environ2920002008
  102. 102. Axelsen KB, Palmgren MG (2001) Inventory of the superfamily of P-type ion pumps in Arabidopsis. Plant Physiol 126: 696–706.KB AxelsenMG Palmgren2001Inventory of the superfamily of P-type ion pumps in Arabidopsis.Plant Physiol126696706
  103. 103. Leung J, Merlot S, Giraudat J (1997) The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. Plant Cell 9: 759–771.J. LeungS. MerlotJ. Giraudat1997The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction.Plant Cell9759771
  104. 104. Nakashima K, Shinwari ZK, Sakuma Y, Seki M, Miura S, et al. (2000) Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression. Plant Mol Biol 42: 657–665.K. NakashimaZK ShinwariY. SakumaM. SekiS. Miura2000Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression.Plant Mol Biol42657665
  105. 105. Abercrombie JM, Halfhill MD, Ranjan P, Rao MR, Saxton AM, et al. (2008) Transcriptional responses of Arabidopsis thaliana plants to As (V) stress. BMC Plant Biol 8: ARTN 87.JM AbercrombieMD HalfhillP. RanjanMR RaoAM Saxton2008Transcriptional responses of Arabidopsis thaliana plants to As (V) stress.BMC Plant Biol8ARTN 87
  106. 106. Babiychuk E, Kushner S, Bellesboix E, Vanmontagu M, Inze D (1995) Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide. J Biol Chem 270: 26224–26231.E. BabiychukS. KushnerE. BellesboixM. VanmontaguD. Inze1995Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide.J Biol Chem2702622426231
  107. 107. Shinozaki K, Yamaguchi-Shinozaki K (2002) ABA signal transduction in drought stress response. Plant Cell Physiol 43: S19–S19.K. ShinozakiK. Yamaguchi-Shinozaki2002ABA signal transduction in drought stress response.Plant Cell Physiol43S19S19
  108. 108. Gong ZZ, Koiwa H, Cushman MA, Ray A, Bufford D, et al. (2001) Genes that are uniquely stress regulated in salt overly sensitive (sos) mutants. Plant Physiol 126: 363–375.ZZ GongH. KoiwaMA CushmanA. RayD. Bufford2001Genes that are uniquely stress regulated in salt overly sensitive (sos) mutants.Plant Physiol126363375