Recently, a pathway-based approach has been developed to evaluate the cumulative contribution of the functionally related genes for genome-wide association studies (GWASs), which may help utilize GWAS data to a greater extent.
In this study, we applied this approach for the GWAS of basal cell carcinoma (BCC) of the skin. We first conducted the BCC GWAS among 1,797 BCC cases and 5,197 controls in Caucasians with 740,760 genotyped SNPs. 115,688 SNPs were grouped into gene transcripts within 20 kb in distance and then into 174 Kyoto Encyclopedia of Genes and Genomes pathways, 205 BioCarta pathways, as well as two positive control gene sets (pigmentation gene set and BCC risk gene set). The association of each pathway with BCC risk was evaluated using the weighted Kolmogorov-Smirnov test. One thousand permutations were conducted to assess the significance.
Both of the positive control gene sets reached pathway p-values<0.05. Four other pathways were also significantly associated with BCC risk: the heparan sulfate biosynthesis pathway (p = 0.007, false discovery rate, FDR = 0.35), the mCalpain pathway (p = 0.002, FDR = 0.12), the Rho cell motility signaling pathway (p = 0.011, FDR = 0.30), and the nitric oxide pathway (p = 0.022, FDR = 0.42).
Citation: Zhang M, Liang L, Xu M, Qureshi AA, Han J (2011) Pathway Analysis for Genome-Wide Association Study of Basal Cell Carcinoma of the Skin. PLoS ONE 6(7): e22760. https://doi.org/10.1371/journal.pone.0022760
Editor: Amanda Ewart Toland, Ohio State University Medical Center, United States of America
Received: May 10, 2011; Accepted: June 28, 2011; Published: July 28, 2011
Copyright: © 2011 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The Nurses' Health Study and the Health Professionals Follow-up Study cohorts are supported by the National Institutes of Health grants CA87969, CA055075, and CA49449. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Recently, genome-wide association studies (GWASs) have often been conducted to investigate the genetic susceptibility to complex diseases, including skin cancer . Cancer of the skin (including both melanoma and non-melanoma skin cancers) is the most common form of cancer, accounting for more than 50% of all cancers. Basal cell carcinoma (BCC) accounts for approximately 80 percent of all non-melanoma skin cancers, and its incidence is increasing . Although GWASs have identified several new loci associated with BCC , , the most significant SNPs that fulfilled a stringent statistical “genome-wide” significance criterion often account for only a small proportion of genetic susceptibility. More attention should be paid to the rest of the genetic information, which may offer a deeper understanding of the genetics of complex diseases . Combining the modest association signals in the GWAS data with information on biological pathways and networks, the emerging pathway-based approaches are designed to utilize the GWAS data to a greater extent and are likely to yield new insights into disease etiology .
It has been suggested that multiple susceptibility genes for a single disease tend to have some related functions or aggregate in certain pathways . For example, some genes associated with BCC risk are related to pigmentation . The pathway-based approach, which evaluates the cumulative contribution of the functionally related genes, may help uncover the associations of genes with small effect sizes that cluster within common biological pathways . Using an approach based on the gene-set enrichment analysis algorithm , pathway analyses have been applied to the GWASs of several complex diseases, and some novel candidate disease-susceptibility pathways have been revealed , , , .
Here we report on the results of a pathway analysis on GWAS of BCC to identify the biological pathways associated with BCC risk, which may provide new insights into the etiology of BCC.
Materials and Methods
This study was approved by the Human Research Committee at the Brigham and Women's Hospital (Boston, MA) with written informed consent from all participants.
Description of Study Populations.
The BCC GWAS comprised 1,797 BCC cases and 5,197 controls, which were from four pre-existing GWAS sets , . The description of BCC GWAS dataset was presented in a manuscript currently in press . Briefly, the four component GWAS sets reflect the type 2 diabetes case-control study nested within the Nurses' Health Study (T2D_NHS, BCC cases = 665, BCC controls = 2,162), the type 2 diabetes case-control study nested within the Health Professionals Follow-up Study (T2D_HPFS, BCC cases = 597, BCC controls = 1,555), the coronary heart disease case-control study nested within the NHS (CHD_NHS, BCC cases = 253, BCC controls = 765), and the coronary heart disease case-control study nested within the HPFS (CHD_HPFS, BCC cases = 282, BCC controls = 715). All the cases were self-reported BCC patients, and we excluded those who had diagnosis of other common cancers before diagnosis of BCC based on common cancers reported by the National Cancer Institute and American Cancer Society. For BCC controls, we also excluded those individuals who had diagnosis of BCC or other common cancers. Those common cancers included melanoma, SCC, breast cancer, endometrial cancer, ovarian cancer, colorectal cancer, bladder cancer, lung cancer, pancreatic cancer, kidney (renal cell) carcinoma, leukemia, Non-Hodgkin Lymphoma, thyroid cancer, and oral cancer. Overlapping individuals among the four GWAS sets were removed. All the individuals in our study are of European ancestry and live in the United States. The study protocol was approved by the Institutional Review Boards of Brigham and Women's Hospital and the Harvard School of Public Health. Detailed descriptions of the study populations for each study are presented in the Methods S1.
Genotyping and Quality Control.
We used the genotype data from four pre-existing GWAS sets (T2D_NHS, T2D_HPFS, CHD_NHS, and CHD_HPFS) . The genotyping was previously conducted using the Affymetrix 6.0 array and the information on genotyping and quality control was previously described . For each study, the detailed information was summarized in the Methods S1.
Associations between each SNP and BCC risk were determined using a multivariable logistic regression model; Age and the first three principal components of genetic variation (Methods S1) were adjusted in the model within each of the four sets. The case-control status of T2D and CHD was also adjusted in the sensitivity analysis. For each SNP, within-cohort association results were combined in an inverse variance–weighted meta-analysis using METAL software .
Pathway data construction
We collected pathway data from the Kyoto Encyclopedia of Genes and Genomes (KEGG) and BioCarta databases; 174 KEGG pathways and 205 BioCarta pathways that contain at least 10 but at most 200 genes represented by qualified SNPs were tested. The cutoff of 10–200 genes was selected as a good balance for gene sets to reduce the multiple-testing issue and to avoid testing overly narrow or broad functional categories. We also designed two positive control gene sets, the pigmentation gene set and BCC risk gene set, to test this approach. We selected the genes based on information gathered by the previous GWAS studies on the pigmentation traits and BCC risk. The pigmentation gene set included SLC45A2, IRF4, EXOC2, TYPR1, TPCN2, TYR, KITLG, SLC24A4, OCA2, MC1R, ASIP, HERC2; and the BCC risk gene set included PADI6, RHOU, SLC45A2, TERT, CLPTM1L, KLF14, CDKN2A, CDKN2B, TYR, KRT5, MC1R, ASIP, PTCH1.
Statistical Method of the Pathway Analysis
We applied the method of Wang et al.  to conduct the pathway-based GWAS analysis. Specifically, we first calculated the statistic value for the association of each SNP with BCC risk. Then, we associated the SNPs located within 20 kb upstream or downstream of gene transcript with each gene, and assigned the highest statistical value among all the SNPs located in this region as representative of this gene. All the genes were assigned to pathways as previously defined. Then the association of each pathway with BCC (denoted by Enrichment Score, ES) was evaluated using the weighted Kolmogorov-Smirnov-like running sum statistic, which reflects the overrepresentation of genes within this pathway at the top of the entire ranked list of genes in the genome. We used a permutation procedure (1,000 permutations, permuting the case-control status and re-calculating the pathway-based ES) to assess the significance of the statistical value. A normalized enrichment score was calculated for each pathway to allow direct comparison of pathways of different sizes. The false discovery rate (FDR) was calculated to keep the proportion of expected false positive findings below a certain threshold . We set the significance level for the pathway analysis as p value<0.05 and FDR<0.5. The detailed algorithm of pathway analysis was described by Wang et al .
Basic characteristics of the 1,797 BCC cases and 5,197 controls in our study are presented in Table S1. As shown in the table, the BCC cases were more likely to have red or blonde hair and lifetime severe sunburns that blistered, and they tended to have poorer ability to tan than the controls (Table S1).
Of the 740,760 genotyped SNPs, 366,550 SNPs located within 20 kb upstream or downstream of gene transcripts were assigned to the genes. Among them, 115,688 SNPs were ultimately assigned to 4,891 genes within the pre-defined pathways (107,521 SNPs to 4,538 genes in the KEGG pathways and 34,301 SNPs to 1,298 genes in the BioCarta pathways). A total of 174 KEGG pathways and 205 BioCarta pathways that contained between 10 and 200 eligible genes were used for the pathway analysis.
Both of the positive control gene sets reached a nominal p-value<0.05 (p = 0.001 for the pigmentation gene set and p = 0.03 for the BCC risk gene set). As shown in Table S2, 10 out of 12 genes in the pigmentation gene set had representative SNPs that reached p value<0.05 for the association with BCC (range 0.02 to 3.4E-7, and the two that did not reach significance had p values of 0.05 and 0.09), and seven out of 13 genes in the BCC risk gene set had SNPs with p value<0.05 (range 0.03 to 3.4E-7, and the remaining six had p values from 0.11 to 0.16).
Besides the two positive control gene sets, 10 out of the 174 KEGG pathways and eight out of the 205 BioCarta pathways reached a significance level of p<0.05, and four also reached a FDR of less than 0.5. Those were the heparan sulfate biosynthesis pathway (p = 0.007, FDR = 0.35), the mCalpain pathway (p = 0.002, FDR = 0.12), the Rho cell motility signaling pathway (p = 0.011, FDR = 0.30), and the nitric oxide pathway (p = 0.022, FDR = 0.42, Table 1). Complete association results for all the pathways in the KEGG database are listed in Table S3 (a), and those in the BioCarta database are listed and Table S3 (b)).
Thirteen out of 19 genes in the heparan sulfate biosynthesis pathway had SNPs with p-value for marginal effect on BCC risk (PBCC)<0.05; the same was true for 14 out of 24 genes in the mCalpain pathway, 16 out of 32 genes in the Rho cell motility signaling pathway, and 16 out of 30 genes in the nitric oxide pathway. The representative SNP of each gene in the four pathways that had PBCC<0.01 are listed in Table 1, and the representative SNPs of all the genes in these pathways are listed in Table S4.
According to the pathway definition in the BioCarta database, there are some overlaps between the mCalpain pathway (24 genes) and the other two pathways: 3 genes (MYLK, MYL2 and TLN1) in the Rho cell motility signaling pathway (32 genes); and 6 genes (PRKACG, PRKACB, PRKAR2B, PRKAR1A, PRKAR1B, PRKAR2A) in the nitric oxide pathway (30 genes). After excluding the 3 genes shared between the mCalpain pathway and the Rho cell motility signaling pathway, the association obviously attenuated for both pathways (p = 0.04 and FDR = 0.61 for the mCalpain pathway, and p = 0.05 and FDR = 0.84 for the Rho cell motility signaling pathway). The association remained similar after excluding the 6 genes shared between the mCalpain pathway (p = 0.003 and FDR = 0.12) and the nitric oxide pathway (p = 0.04 and FDR = 0.76). No other overlap was found among the remaining pathways. In addition, based on the concern that the case-control status of T2D and CHD may introduce potential bias to the associations between the SNPs and BCC risk, a sensitivity analysis was conducted by adjusting for the case-control status. As a result, no substantial change was detected for the associations before and after the adjustment.
Genome-wide association studies primarily focus on marginal effects for individual markers. Given that some of the susceptibility genes for a given disease share related functions, a pathway-based approach that evaluates the cumulative contribution of the functionally related genes can utilize GWAS data to a greater extent. This method has recently shown its potential strength in application to GWASs in several complex diseases , , , . Here we report the results of a pathway analysis to investigate the GWAS associations for BCC. Gene-sets that contained pigmentation genes or BCC risk genes identified from previous GWASs were significantly associated with BCC in our pathway analysis, which suggests that the pathway-based approach can effectively incorporate the modest effects of genes with related functions. In addition, we identified four other pathways significantly associated with BCC risk as defined by the KEGG and BioCarta databases: the heparan sulfate biosynthesis pathway, the mCalpain pathway, the Rho cell motility signaling pathway, and the nitric oxide pathway.
The mCalpain pathway ranked first among the 205 BioCarta pathways tested in this study (p = 0.002 and FDR = 0.18). Calpain is a class of Ca2+-dependent cysteine proteases with two major ubiquitously expressed isoforms: μ-calpain (or calpain I) and m-calpain (or calpain II). One of the most carefully studied functions of the calpains is the regulation of integrin-mediated cell migration . mCalpain is believed to be membrane bound and functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals , , which may play a role in the invasion of tumor cells into surrounding stroma during the development of BCC. In addition to cell migration, a number of protein substrates of calpains have been identified as playing very important roles in cell proliferation, apoptosis, and cell cycle control , , , , . The assessed m-calpain expression level appeared to be inversely correlated to apoptotic activity in various cell types , , and the over-expression of m-calpain has been detected in human cancer . To date, calpain inhibitors have been used widely to trigger apoptosis in various cancer cell lines . However, as for BCC, only one study has reported that the expression of u-calpain in BCCs was markedly elevated compared to normal human skin, and no difference was found for m-calpain . In our study, although we detected only a weak association between the m-calpain polymorphisms and BCC risk (representative p = 0.05), a stronger association was identified for the m-calpain pathway, which suggests that m-calpain has its effects through interaction with other genes in the pathway.
The other two pathways associated with BCC risk in the BioCarta database were the Rho cell motility signaling pathway and the nitric oxide pathway. After excluding the genes shared with the mCalpain pathway, the association for the Rho cell motility signaling pathway became non-significant, suggesting that the association was driven by the genes shared with the mCalpain pathway. There is evidence from previous study that calpain mediates integrin-induced signaling at a point upstream of Rho family members . It is certainly plausible that BCC tumorigenesis requires the mCalpain pathway for local invasion into the epidermis and through the basement membrane zone into the dermis. Another plausible explanation is an integrin-mediated immune response that requires adhesion of circulating anti-tumor T cells to the dermal endothelial cells followed by migration of T cells into tissues – an aberration of a T cell response to BCC may allow the tumor to grow unabated in skin.
The association of the nitric oxide pathway remained significant after excluding the genes shared with the mCalpain pathway, which suggests an independent effect. Nitric oxide (NO) is a signaling molecule that plays a critical role in vasodilatation, neurotransmission and immunity, and has been shown to be cytostatic or cytotoxic for cancer cells . NO is also produced in the skin and its associated microvasculature, and has complex implications in skin homeostasis and diseases . The NO pathway in the BioCarta database focuses mainly on the sub-network of NOS3. NOS3, also named as endothelial cell constitutive NOS or eNOS, plays an important role in the regulation of vascular endothelial function , and the expression of NOS3 was reported to be down-regulated in basal cell carcinomas when compared with normal skin . A significant association was also detected between NOS3 polymorphisms and BCC risk in our study (representative p = 0.01). A characteristic clinical feature of BCCs is a translucent papule or nodule with prominent telangiectasia, presumably an angiogenic response propagated by the growing skin cancer. A NOS-predominant BCC may potentially be able to attract a risk blood supply for continued growth and NOS (via NOS inhibitors) may be considered a therapeutic target.
As for the KEGG pathways, the heparan sulfate biosynthesis pathway was significantly associated with BCC risk (p = 0.007 and FDR = 0.35). Heparan sulfate (HS) is a glycosaminoglycan covalently attached to serine residues in a proteoglycan core protein. Heparan sulfate proteoglycans (HSPGs) have been known to play vital roles in every step of tumor progression: allowing cancer cells to proliferate, escape from immune response, invade neighboring tissues, and metastasize to distant sites . Several cancers including melanoma have shown aberrant modulation of several key HS biosynthetic enzymes , , , . However, no study has been reported on BCC, and our finding suggests the potential importance of this pathway in the etiology of BCC, relevant in the case of nodular or morpheaform BCCs where dermal invasion is commonly seen.
There are some limitations to our approach. First, current knowledge on human gene functions is incomplete, and the pre-defined pathways cannot comprehensively represent the functionally related genes in the human genome. We used the KEGG and BioCarta pathways in this study, which represent relatively well-defined known biological pathways. To minimize multiple-testing concerns, we did not include some other pathway databases with more broad functional categories such as the Gene Ontology (GO) , . Secondly, the assignment of SNPs to genes in physical proximity may not always be accurate. A gene may be regulated in trans by genetic variants that are physically distant from the structural gene . Third, given the modest sample size, the FDRs of the identified pathways are not particularly low. Further replication using additional samples and functional investigation would be required to illustrate the true effect behind these pathways.
In conclusion, we applied a pathway-based approach to further explore the GWAS data of BCC and identified several pathways associated with BCC risk, which was missed in the traditional GWAS. These findings may suggest new insights into the etiology of BCC upon further replication. Functional studies are also warranted to characterize the biological functions of these pathways in BCC development.
Characteristics of BCC cases and controls in this study.
The representative SNP information for the two positive control pathways.
Association results for the 174 KEGG pathways and 205 BioCarta pathways. (a) Association results for the 174 KEGG pathways; (b) Association results for the 205 BioCarta pathways.
The representative SNP information for the four pathways with significant enrichment in the BCC GWAS.
We thank Dr. David Hunter, Dr. Frank B. Hu, and Dr. Eric B. Rimm for their work on the five GWAS sets. We thank Dr. Hongmei Nan for her work in BCC GWAS dataset construction. We thank the participants in the Nurses' Health Study and the Health Professionals Follow-Up Study for their dedication and commitment. We also thank the following state cancer registries for their help: AL, AZ, AR, CA, CO, CT, DE, FL, GA, ID, IL, IN, IA, KY, LA, ME, MD, MA, MI, NE, NH, NJ, NY, NC, ND, OH, OK, OR, PA, RI, SC, TN, TX, VA, WA, WY.
Conceived and designed the experiments: MZ LL JH. Performed the experiments: JH. Analyzed the data: MZ LL MX JH. Contributed reagents/materials/analysis tools: MZ LL JH. Wrote the paper: MZ LL AQ JH.
- 1. Gerstenblith MR, Shi J, Landi MT (2010) Genome-wide association studies of pigmentation and skin cancer: a review and meta-analysis. Pigment Cell Melanoma Res 23: 587–606.MR GerstenblithJ. ShiMT Landi2010Genome-wide association studies of pigmentation and skin cancer: a review and meta-analysis.Pigment Cell Melanoma Res23587606
- 2. de Vries E, Louwman M, Bastiaens M, de Gruijl F, Coebergh JW (2004) Rapid and continuous increases in incidence rates of basal cell carcinoma in the southeast Netherlands since 1973. J Invest Dermatol 123: 634–638.E. de VriesM. LouwmanM. BastiaensF. de GruijlJW Coebergh2004Rapid and continuous increases in incidence rates of basal cell carcinoma in the southeast Netherlands since 1973.J Invest Dermatol123634638
- 3. Stacey SN, Gudbjartsson DF, Sulem P, Bergthorsson JT, Kumar R, et al. (2008) Common variants on 1p36 and 1q42 are associated with cutaneous basal cell carcinoma but not with melanoma or pigmentation traits. Nat Genet 40: 1313–1318.SN StaceyDF GudbjartssonP. SulemJT BergthorssonR. Kumar2008Common variants on 1p36 and 1q42 are associated with cutaneous basal cell carcinoma but not with melanoma or pigmentation traits.Nat Genet4013131318
- 4. Stacey SN, Sulem P, Masson G, Gudjonsson SA, Thorleifsson G, et al. (2009) New common variants affecting susceptibility to basal cell carcinoma. Nat Genet 41: 909–914.SN StaceyP. SulemG. MassonSA GudjonssonG. Thorleifsson2009New common variants affecting susceptibility to basal cell carcinoma.Nat Genet41909914
- 5. Manolio TA, Collins FS, Cox NJ, Goldstein DB, Hindorff LA, et al. (2009) Finding the missing heritability of complex diseases. Nature 461: 747–753.TA ManolioFS CollinsNJ CoxDB GoldsteinLA Hindorff2009Finding the missing heritability of complex diseases.Nature461747753
- 6. Pedroso I (2010) Gaining a pathway insight into genetic association data. Methods Mol Biol 628: 373–382.I. Pedroso2010Gaining a pathway insight into genetic association data.Methods Mol Biol628373382
- 7. Holmans P, Green EK, Pahwa JS, Ferreira MA, Purcell SM, et al. (2009) Gene ontology analysis of GWA study data sets provides insights into the biology of bipolar disorder. Am J Hum Genet 85: 13–24.P. HolmansEK GreenJS PahwaMA FerreiraSM Purcell2009Gene ontology analysis of GWA study data sets provides insights into the biology of bipolar disorder.Am J Hum Genet851324
- 8. Subramanian A, Tamayo P, Mootha VK, Mukherjee S, Ebert BL, et al. (2005) Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci U S A 102: 15545–15550.A. SubramanianP. TamayoVK MoothaS. MukherjeeBL Ebert2005Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles.Proc Natl Acad Sci U S A1021554515550
- 9. Menashe I, Maeder D, Garcia-Closas M, Figueroa JD, Bhattacharjee S, et al. (2010) Pathway analysis of breast cancer genome-wide association study highlights three pathways and one canonical signaling cascade. Cancer Res 70: 4453–4459.I. MenasheD. MaederM. Garcia-ClosasJD FigueroaS. Bhattacharjee2010Pathway analysis of breast cancer genome-wide association study highlights three pathways and one canonical signaling cascade.Cancer Res7044534459
- 10. Perry JR, McCarthy MI, Hattersley AT, Zeggini E, Weedon MN, et al. (2009) Interrogating type 2 diabetes genome-wide association data using a biological pathway-based approach. Diabetes 58: 1463–1467.JR PerryMI McCarthyAT HattersleyE. ZegginiMN Weedon2009Interrogating type 2 diabetes genome-wide association data using a biological pathway-based approach.Diabetes5814631467
- 11. Wang K, Li M, Bucan M (2007) Pathway-Based Approaches for Analysis of Genomewide Association Studies. Am J Hum Genet 81: K. WangM. LiM. Bucan2007Pathway-Based Approaches for Analysis of Genomewide Association Studies.Am J Hum Genet81
- 12. Wang K, Zhang H, Kugathasan S, Annese V, Bradfield JP, et al. (2009) Diverse genome-wide association studies associate the IL12/IL23 pathway with Crohn Disease. Am J Hum Genet 84: 399–405.K. WangH. ZhangS. KugathasanV. AnneseJP Bradfield2009Diverse genome-wide association studies associate the IL12/IL23 pathway with Crohn Disease.Am J Hum Genet84399405
- 13. Qi L, Cornelis MC, Kraft P, Stanya KJ, Linda Kao WH, et al. (2010) Genetic variants at 2q24 are associated with susceptibility to type 2 diabetes. Hum Mol Genet 19: 2706–2715.L. QiMC CornelisP. KraftKJ StanyaWH Linda Kao2010Genetic variants at 2q24 are associated with susceptibility to type 2 diabetes.Hum Mol Genet1927062715
- 14. Rimm EB, Giovannucci EL, Willett WC, Colditz GA, Ascherio A, et al. (1991) Prospective study of alcohol consumption and risk of coronary disease in men. Lancet 338: 464–468.EB RimmEL GiovannucciWC WillettGA ColditzA. Ascherio1991Prospective study of alcohol consumption and risk of coronary disease in men.Lancet338464468
- 15. Nan H, Xu M, Kraft P, Qureshi AA, Chen C, et al. (2011) Genome-wide association study identifies novel alleles associated with risk of cutaneous basal cell carcinoma and squamous cell carcinoma. Hum Mol Genet. H. NanM. XuP. KraftAA QureshiC. Chen2011Genome-wide association study identifies novel alleles associated with risk of cutaneous basal cell carcinoma and squamous cell carcinoma.Hum Mol Genet. In press. . In press.
- 16. METAL software. METAL software.Available: http://www.sph.umich.edu/csg/abecasis/Metal/index.html. Accessed 2010 Apr 15. Available: http://www.sph.umich.edu/csg/abecasis/Metal/index.html. Accessed 2010 Apr 15.
- 17. Reiner A, Yekutieli D, Benjamini Y (2003) Identifying differentially expressed genes using false discovery rate controlling procedures. Bioinformatics 19: 368–375.A. ReinerD. YekutieliY. Benjamini2003Identifying differentially expressed genes using false discovery rate controlling procedures.Bioinformatics19368375
- 18. Glading A, Lauffenburger DA, Wells A (2002) Cutting to the chase: calpain proteases in cell motility. Trends Cell Biol 12: 46–54.A. GladingDA LauffenburgerA. Wells2002Cutting to the chase: calpain proteases in cell motility.Trends Cell Biol124654
- 19. Glading A, Uberall F, Keyse SM, Lauffenburger DA, Wells A (2001) Membrane proximal ERK signaling is required for M-calpain activation downstream of epidermal growth factor receptor signaling. J Biol Chem 276: 23341–23348.A. GladingF. UberallSM KeyseDA LauffenburgerA. Wells2001Membrane proximal ERK signaling is required for M-calpain activation downstream of epidermal growth factor receptor signaling.J Biol Chem2762334123348
- 20. Atencio IA, Ramachandra M, Shabram P, Demers GW (2000) Calpain inhibitor 1 activates p53-dependent apoptosis in tumor cell lines. Cell Growth Differ 11: 247–253.IA AtencioM. RamachandraP. ShabramGW Demers2000Calpain inhibitor 1 activates p53-dependent apoptosis in tumor cell lines.Cell Growth Differ11247253
- 21. Carragher NO, Frame MC (2002) Calpain: a role in cell transformation and migration. Int J Biochem Cell Biol 34: 1539–1543.NO CarragherMC Frame2002Calpain: a role in cell transformation and migration.Int J Biochem Cell Biol3415391543
- 22. Li G, Iyengar R (2002) Calpain as an effector of the Gq signaling pathway for inhibition of Wnt/beta -catenin-regulated cell proliferation. Proc Natl Acad Sci U S A 99: 13254–13259.G. LiR. Iyengar2002Calpain as an effector of the Gq signaling pathway for inhibition of Wnt/beta -catenin-regulated cell proliferation.Proc Natl Acad Sci U S A991325413259
- 23. Xu Y, Mellgren RL (2002) Calpain inhibition decreases the growth rate of mammalian cell colonies. J Biol Chem 277: 21474–21479.Y. XuRL Mellgren2002Calpain inhibition decreases the growth rate of mammalian cell colonies.J Biol Chem2772147421479
- 24. Croall DE, DeMartino GN (1991) Calcium-activated neutral protease (calpain) system: structure, function, and regulation. Physiol Rev 71: 813–847.DE CroallGN DeMartino1991Calcium-activated neutral protease (calpain) system: structure, function, and regulation.Physiol Rev71813847
- 25. Benetti R, Del Sal G, Monte M, Paroni G, Brancolini C, et al. (2001) The death substrate Gas2 binds m-calpain and increases susceptibility to p53-dependent apoptosis. Embo J 20: 2702–2714.R. BenettiG. Del SalM. MonteG. ParoniC. Brancolini2001The death substrate Gas2 binds m-calpain and increases susceptibility to p53-dependent apoptosis.Embo J2027022714
- 26. Lakshmikuttyamma A, Selvakumar P, Kanthan R, Kanthan SC, Sharma RK (2004) Overexpression of m-calpain in human colorectal adenocarcinomas. Cancer Epidemiol Biomarkers Prev 13: 1604–1609.A. LakshmikuttyammaP. SelvakumarR. KanthanSC KanthanRK Sharma2004Overexpression of m-calpain in human colorectal adenocarcinomas.Cancer Epidemiol Biomarkers Prev1316041609
- 27. Guan N, Korukonda R, Hurh E, Schmittgen TD, Donkor IO, et al. (2006) Apoptosis induced by novel aldehyde calpain inhibitors in human tumor cell lines. Int J Oncol 29: 655–663.N. GuanR. KorukondaE. HurhTD SchmittgenIO Donkor2006Apoptosis induced by novel aldehyde calpain inhibitors in human tumor cell lines.Int J Oncol29655663
- 28. Reichrath J, Welter C, Mitschele T, Classen U, Meineke V, et al. (2003) Different expression patterns of calpain isozymes 1 and 2 (CAPN1 and 2) in squamous cell carcinomas (SCC) and basal cell carcinomas (BCC) of human skin. J Pathol 199: 509–516.J. ReichrathC. WelterT. MitscheleU. ClassenV. Meineke2003Different expression patterns of calpain isozymes 1 and 2 (CAPN1 and 2) in squamous cell carcinomas (SCC) and basal cell carcinomas (BCC) of human skin.J Pathol199509516
- 29. Kulkarni S, Saido TC, Suzuki K, Fox JE (1999) Calpain mediates integrin-induced signaling at a point upstream of Rho family members. J Biol Chem 274: 21265–21275.S. KulkarniTC SaidoK. SuzukiJE Fox1999Calpain mediates integrin-induced signaling at a point upstream of Rho family members.J Biol Chem2742126521275
- 30. Pacher P, Beckman JS, Liaudet L (2007) Nitric oxide and peroxynitrite in health and disease. Physiol Rev 87: 315–424.P. PacherJS BeckmanL. Liaudet2007Nitric oxide and peroxynitrite in health and disease.Physiol Rev87315424
- 31. Bruch-Gerharz D, Ruzicka T, Kolb-Bachofen V (1998) Nitric oxide and its implications in skin homeostasis and disease - a review. Arch Dermatol Res 290: 643–651.D. Bruch-GerharzT. RuzickaV. Kolb-Bachofen1998Nitric oxide and its implications in skin homeostasis and disease - a review.Arch Dermatol Res290643651
- 32. Villiotou V, Deliconstantinos G (1995) Nitric oxide, peroxynitrite and nitroso-compounds formation by ultraviolet A (UVA) irradiated human squamous cell carcinoma: potential role of nitric oxide in cancer prognosis. Anticancer Res 15: 931–942.V. VilliotouG. Deliconstantinos1995Nitric oxide, peroxynitrite and nitroso-compounds formation by ultraviolet A (UVA) irradiated human squamous cell carcinoma: potential role of nitric oxide in cancer prognosis.Anticancer Res15931942
- 33. Brennan PA, Umar T, Bowden J, Hobkirk A, Spedding AV, et al. (2000) Nitric oxide synthase expression is downregulated in basal cell carcinoma of the head and neck. Br J Oral Maxillofac Surg 38: 633–636.PA BrennanT. UmarJ. BowdenA. HobkirkAV Spedding2000Nitric oxide synthase expression is downregulated in basal cell carcinoma of the head and neck.Br J Oral Maxillofac Surg38633636
- 34. Raman K, Kuberan B (2010) Chemical Tumor Biology of Heparan Sulfate Proteoglycans. Curr Chem Biol 4: 20–31.K. RamanB. Kuberan2010Chemical Tumor Biology of Heparan Sulfate Proteoglycans.Curr Chem Biol42031
- 35. Cohen IR, Murdoch AD, Naso MF, Marchetti D, Berd D, et al. (1994) Abnormal expression of perlecan proteoglycan in metastatic melanomas. Cancer Res 54: 5771–5774.IR CohenAD MurdochMF NasoD. MarchettiD. Berd1994Abnormal expression of perlecan proteoglycan in metastatic melanomas.Cancer Res5457715774
- 36. Iozzo RV, Cohen IR, Grassel S, Murdoch AD (1994) The biology of perlecan: the multifaceted heparan sulphate proteoglycan of basement membranes and pericellular matrices. Biochem J 302(Pt 3): 625–639.RV IozzoIR CohenS. GrasselAD Murdoch1994The biology of perlecan: the multifaceted heparan sulphate proteoglycan of basement membranes and pericellular matrices.Biochem J302Pt 3625639
- 37. Smetsers TF, van de Westerlo EM, ten Dam GB, Clarijs R, Versteeg EM, et al. (2003) Localization and characterization of melanoma-associated glycosaminoglycans: differential expression of chondroitin and heparan sulfate epitopes in melanoma. Cancer Res 63: 2965–2970.TF SmetsersEM van de WesterloGB ten DamR. ClarijsEM Versteeg2003Localization and characterization of melanoma-associated glycosaminoglycans: differential expression of chondroitin and heparan sulfate epitopes in melanoma.Cancer Res6329652970
- 38. Sharma B, Handler M, Eichstetter I, Whitelock JM, Nugent MA, et al. (1998) Antisense targeting of perlecan blocks tumor growth and angiogenesis in vivo. J Clin Invest 102: 1599–1608.B. SharmaM. HandlerI. EichstetterJM WhitelockMA Nugent1998Antisense targeting of perlecan blocks tumor growth and angiogenesis in vivo.J Clin Invest10215991608
- 39. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, et al. (2000) Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 25: 25–29.M. AshburnerCA BallJA BlakeD. BotsteinH. Butler2000Gene ontology: tool for the unification of biology. The Gene Ontology Consortium.Nat Genet252529
- 40. Zhong H, Yang X, Kaplan LM, Molony C, Schadt EE (2010) Integrating pathway analysis and genetics of gene expression for genome-wide association studies. Am J Hum Genet 86: 581–591.H. ZhongX. YangLM KaplanC. MolonyEE Schadt2010Integrating pathway analysis and genetics of gene expression for genome-wide association studies.Am J Hum Genet86581591
- 41. Schadt EE, Molony C, Chudin E, Hao K, Yang X, et al. (2008) Mapping the genetic architecture of gene expression in human liver. PLoS Biol 6: e107.EE SchadtC. MolonyE. ChudinK. HaoX. Yang2008Mapping the genetic architecture of gene expression in human liver.PLoS Biol6e107