Studies of the Y chromosome in primates, rodents and carnivores provide compelling evidence that the male specific region of Y (MSY) contains functional genes, many of which have specialized roles in spermatogenesis and male-fertility. Little similarity, however, has been found between the gene content and sequence of MSY in different species. This hinders the discovery of species-specific male fertility genes and limits our understanding about MSY evolution in mammals. Here, a detailed MSY gene catalogue was developed for the horse – an odd-toed ungulate. Using direct cDNA selection from horse testis, and sequence analysis of Y-specific BAC clones, 37 horse MSY genes/transcripts were identified. The genes were mapped to the MSY BAC contig map, characterized for copy number, analyzed for transcriptional profiles by RT-PCR, examined for the presence of ORFs, and compared to other mammalian orthologs. We demonstrate that the horse MSY harbors 20 X-degenerate genes with known orthologs in other eutherian species. The remaining 17 genes are acquired or novel and have so far been identified only in the horse or donkey Y chromosomes. Notably, 3 transcripts were found in the heterochromatic part of the Y. We show that despite substantial differences between the sequence, gene content and organization of horse and other mammalian Y chromosomes, the functions of MSY genes are predominantly related to testis and spermatogenesis. Altogether, 10 multicopy genes with testis-specific expression were identified in the horse MSY, and considered likely candidate genes for stallion fertility. The findings establish an important foundation for the study of Y-linked genetic factors governing fertility in stallions, and improve our knowledge about the evolutionary processes that have shaped Y chromosomes in different mammalian lineages.
Citation: Paria N, Raudsepp T, Pearks Wilkerson AJ, O'Brien PCM, Ferguson-Smith MA, Love CC, et al. (2011) A Gene Catalogue of the Euchromatic Male-Specific Region of the Horse Y Chromosome: Comparison with Human and Other Mammals. PLoS ONE 6(7): e21374. https://doi.org/10.1371/journal.pone.0021374
Editor: Laszlo Orban, Temasek Life Sciences Laboratory, Singapore
Received: January 21, 2011; Accepted: May 27, 2011; Published: July 25, 2011
Copyright: © 2011 Paria et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The work was supported by USDA grant CSREES-#2006-04801, and partially by Grayson-Jockey Club, American Quarter Horse and LINK Endowment Research Foundations. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Mammalian Y chromosome stands out from the rest of the genome because it is male specific, constitutively haploid and exhibits unique structural and functional features –. Typically, it is one of the smallest chromosomes in the genome and harbors both the pseudoautosomal and the male-specific genes.
The male specific region of the Y (MSY) has been sequenced in the human (Homo sapiens, HSA)  and the chimpanzee (Pan troglodytes, PTR)  leading to systematic discovery and mapping of male-specific genes. Detailed functional characterization of Y chromosome genes has been carried out also in mouse (Mus musculus, MMU) –. Additionally, Y chromosome genes have been mapped and/or functionally analyzed in carnivores – and cattle –, and to a limited extent in river buffalo , pig –, sheep – and rabbit . These studies clearly show that mammalian Y chromosomes carry a rich repertoire of functional genes, several of which might play a role in spermatogenesis and male fertility.
The human MSY euchromatin contains 27 gene families corresponding to 78 transcriptional units . Another eight genes with open reading frames (ORFs) are located in a small euchromatic island in the HSAY pericentromeric heterochromatin . Compared to humans, the PTRY has lost large fractions of MSY protein-coding genes in the course of evolution, and has retained only 18 gene families with 37 transcriptional units . Gene catalogues for other mammalian Y chromosomes are less comprehensive: 53 genes or gene families have been identified in mouse (http://www.ncbi.nlm.nih.gov/mapview/), 19 in cat –, over 13 in cattle – and at least 16 in gorilla . The mouse Y chromosome is functionally well studied, and provides compelling evidence that Y-linked genes are involved in multiple processes during spermiogenesis, including sperm motility, and the development and function of the acrosome , . In cat (Felis catus, FCA), the MSY genes have been analyzed for expression profiles and possible involvement in male fertility –. The organization of FCAY resembles that of MMUY as single copy genes contain on the short arm, while highly amplified testis-specific gene families are distributed all over the long arm . Considerable progress has been made in mapping and analyzing the cattle (Bos taurus, BTA) Y chromosome including the construction of basic RH  and cytogenetic maps , and identifying over 10 X-degenerate genes and three lineage-specific gene families, viz., ZNF280BY, ZNF280AY and PRAMEY -. Among other domestic species, pig is probably the next to have a high resolution Y chromosome map and sequencing data. This is because Pig Genome Sequencing project is based on a whole genome BAC fingerprint contig including the Y chromosome .
Human and chimpanzee MSY sequences demonstrate that most of the Y chromosome genes fall into two sequence classes: ampliconic (multicopy gene families expressed predominantly or exclusively in testis) and X-degenerate (single copy ancestral homologues of X-linked genes, most of which are expressed ubiquitously) , . However, there are pronounced differences between mammalian Y chromosomes studied so far, and MSY sequences can differ even between closely related species. For example, over 30% of the chimpanzee MSY sequence has no counterpart in the human MSY, and the PTRY has only two-thirds as many distinct genes as HSAY . Comparisons between species show that a core set of X-degenerate genes are shared between mammals, while multicopy genes, which are typically Y-borne or acquired from other parts of the genome, are species- or lineage-specific , , , . Furthermore, the majority of testis expressed, and potentially male fertility-related MSY genes are restricted to a species or a group of related species –. The unusual features of the MSY call for the systematic discovery of Y-linked genes in a larger number of mammalian species, mainly to improve our knowledge about the evolutionary processes that have shaped the MSY gene content in different lineages, and to identify species-specific male fertility genes.
While stallion fertility is of prime importance to the equine industry, the current knowledge about Y-linked fertility factors in horses is limited, thus justifying the launch of systematic Y chromosome research in this species. Given that the domestic horse (Equus caballus, ECA) is a eutherian mammal that is evolutionarily distant from primates, rodents and carnivores (http://www.timetree.org/), the findings will bear also comparative value.
The first gene loci, viz., TSPY , ZFY  AMELY  and SRY  were assigned to the horse Y chromosome (ECAY) indirectly by PCR. Thereafter, SRY, ZFY, and STS were synteny mapped to ECAY by somatic cell hybrid analysis , and the location of SRY and ZFY was further refined using FISH . Systematic discovery of ECAY genes, however, started in 2004 when the location and linear order of eight MSY genes was determined using a combination of radiation hybrid analysis, FISH, and BAC contig mapping . This was followed by a detailed mapping of the pseudoautosomal region . Altogether, these studies have identified 28 Y-linked genes in horses of which majority are pseudoautosomal , while only 9 are present in the MSY . In the present study, we employed direct cDNA selection  from equine testis, followed by isolation and analysis of MSY specific cDNA clones to obtain a collection of male-specific genes in the horse. The genes were tentatively assigned to the ECAY contig map, and analyzed for various structural and functional features. The gene catalogue of equine MSY allowed us to make evolutionary inferences with other eutherian species, and to identify Y-linked candidate genes for stallion fertility.
Results and Discussion
Discovery of horse Y chromosome genes and transcripts
We selected cDNAs from equine testis using horse Y chromosome specific composite probes as selectors, following the methodology previously described for human – and cat –. The purity of the selectors (the flow sorted ECAY and MSY BAC pools) was confirmed by FISH (Fig. 1a, b). After selection, a plasmid library enriched with ECAY cDNA sequences was constructed. We picked 2,400 clones for Sanger dideoxy sequencing, and obtained 1,678 quality sequences that were assembled into 180 contigs and 100 singletons. PCR analysis using contig/singleton-specific primers on male and female genomic DNA showed that 30 contigs and 74 singletons were male specific whereas 150 contigs and 26 singletons amplified both from male and female genomic DNA. Majority of the latter showed significant similarity to known autosomal genes, as well as to autosomal and X-chromosome genomic assemblies, and were removed from further analysis.
a. painting-like signal with flow-sorted Y probe on ECAY eu- and heterochromatin; b. painting-like signal with a probe comprising of 33 MSY BAC clones on ECAY euchromatin; c. and d. distinct signals with UBE1Y cDNA in metaphase and interphase Y chromosome; e. and f. painting-like signals with ZNF33bY cDNA on ECAY and ECAXq21 heterochromatin in metaphase and interphase. Scale bar, 1 µm.
Fourty-four male-female specific cDNA sequences that did not show any significant similarity with known autosomal genes were analyzed by PCR on BAC clones that form the minimum tiling path of the equine pseudoautosomal region (PAR) . No equine PAR transcripts were recovered. Given that a comparable cDNA selection procedure identified all 9 human PAR genes known at the time , the results in horse were unexpected. It is, though, possible that some genomic assemblies corresponding to the X chromosome as detected by BLAST analysis, contained PAR genes which remained unidentified. Therefore, the failure to recover PAR genes in this study is likely because of biased data analysis and not due to low efficacy of cDNA selection procedure.
We identified 321 (19%) cDNA sequences to be male specific by PCR amplification of male and female genomic DNAs using cDNA-specific primers. Among these we found significant (<1e-8) similarity with 13 mammalian MSY and 3 autosomal genes (Table S1). PCR primers for the latter, i.e. EIF3CY, RPS3AY, and ZNF33bY, amplified two distinct products from genomic DNA: a larger autosomal product, which was present in males and females, and a smaller male specific product (Fig. S1). DNA sequencing showed that the two bands differed by a small internal deletion in the Y-derived sequence, though it was not possible to design exclusively male-specific primers. Ten male-specific transcripts were considered novel and horse-specific because no sequence similarity was found in any other species studied so far. Following transcriptional analysis (see below), these sequences were labeled as ETSTY1-6 (Equine Testis-Specific Transcript on Y) and ETY1-4 (Equine Transcript on Y) indicating whether they had testis-specific or broader tissue expression profiles (Table 1). Finally, we found cDNA sequences with a weak similarity (>1e-8) to mitochondrial MT-ND1, autosomal RFX5 and X-degenerate EIF1AY genes. Thus, these annotations remained tentative.
Although the search for expressed sequences by direct selection was systematic, some previously mapped MSY genes  escaped detection. Thus, 8 genes (Table S1) were identified exclusively from MSY BAC clones by PCR with gene specific primers , or by analyzing BAC end or whole BAC  sequences. Because cDNA selection retrieves expressed sequences from a particular tissue, sequences which are not transcribed in this tissue, or have a low relative transcription rate, remain undetected. Indeed, AMELY which is known to be expressed only in developing tooth buds , was not recovered from equine testis cDNA. Further, it is possible that transcription levels of ATP6V0CY, EIF2s3Y, MAP3K7IP3Y, UTY, and TBL1Y in horse testis were too low for detection, while STS-Y and KAL1Y, like in human , might be Y-linked pseudogenes in the horse. Altogether, we identified a total of 37 MSY genes and transcripts in the horse, 29 of them were isolated by direct cDNA selection while 8 genes were discovered by BAC analysis.
The horse MSY gene map
The current BAC contig map of the horse MSY – comprises of 197 BAC clones which are tentatively arranged into 5 contigs (Fig. 2). In this study, 183 of these BACs were used as selectors (Table S2) in the cDNA selection procedure, to isolate expressed MSY sequences from testis. Primers for 29 of the MSY genes were amplified by PCR using DNA of each BAC clone as template, and the location of 26 genes in the 5 contigs was determined (Fig. 2). This confirmed our previous map data – and added 19 new genes to the map. Sequences of ZNF33bY, ETY3 and ETSTY6 were not present in the existing BACs, thus new clones were isolated from the BAC library. All new BACs mapped by FISH and STS content analysis (data not shown) to ECAY heterochromatin adjacent to contig I (Fig 2). Map locations of the 8 genes that were identified by STS content and BAC sequence analysis (see above) were known before. Therefore, the current gene map of horse MSY consists of 37 genes/transcripts that are assigned to 5 BAC contigs and the heterochromatic region (Fig. 2). The precise order of these markers, however, remains tentative, and will be ascertained once the contig map is complete.
A G-banded ideogram (ISCNH 1997) of the horse Y chromosome (ECAY) is shown at the top. Positive G-bands are black, negative G-bands are white and the centromere is shown in grey color. Horizontal lines below the ideogram demarcate the span of the euchromatic and male specific regions of Y. A schematic of ECAY contig map  is at the bottom. White blocks with Roman numerals demarcate the currently available five BAC contigs. Heterochromatic region is shown at the far left and the pseudoautosomal region at the far right. The approximate locations of the 37 MSY genes are shown at the top of the map. Markers in bold were mapped in this study. Arrowheads with Ycen and Ytel show the directions towards the centromere and telomere, respectively.
Gene copy numbers – cDNA FISH
The sensitivity of FISH is limited, therefore it is usually not possible to see signals produced by short (<1000 bp) cDNA sequences –, unless these sequences are present in multiple copies. The average size of the cDNA sequences isolated in this study ranged between 300–850 bp. Most of the genes/transcripts that were mapped by STS content analysis to the multicopy region (Fig. 2) produced strong signals by cDNA FISH (Fig. 1c, d; Table 1), thus confirming the multicopy nature of these sequences. The only exception was SRY cDNA which was mapped in a BAC clone harboring multicopy genes TSPY, RBMY and YIR2 but did not produce any signal by cDNA FISH. Therefore, we infer that SRY is a single copy gene in horse which is embedded within multicopy sequences. In contrast to the multicopy region, no FISH signals were observed with the cDNAs of genes located in the proximal part of contig I, and in contigs II–V (Fig. 2). Finally, the cDNA clones of ETY3, ETSTY6 and ZNF33bY produced painting-like signals in ECAY and ECAXq21 heterochromatin (Fig. 1e, f) indicating that these sequences are highly amplified. Overall, we identified 15 ampliconic genes/transcripts – 12 in the multicopy region and 3 in ECAY heterochromatin (Fig. 2).
Transcriptional activity and profiles of MSY genes in stallion testis
Direct selection yielded a range of cDNA sequences for different MSY genes indicating the relative transcriptional activity of these genes in stallion testis. For example, only one transcript was retrieved for CUL4BY, ETY1-4, MT-ND1, NLGN4Y, RBMY, RFX5Y, RPS3AY, TMSB4Y, and YIR2. In contrast, 54 and 43 cDNA sequences were found for UBE1Y and SRY, respectively (Table 1). We noticed that SRY was recovered in only those direct selection experiments where testis cDNA and Cot-1 ratio was low (1∶2). We re-analyzed the 1,420 bp sequence of the single coding exon  of SRY and discovered a 20 bp LTR repeat. This repeat probably anneals to Cot-1 DNA and eliminates SRY from selection experiments with high cDNA/Cot-1 ratios (1∶7.5). Further sequence analysis revealed that exonic LTR and/or simple repeats are present also in mouse, rat, rabbit, cat, dog and donkey SRY genes (Ensembl, http://www.ensembl.org/index.html; NCBI Entrez Nucleotide, http://www.ncbi.nlm.nih.gov/sites/entrez?db=nucleotide), and have not been reported before. The evolutionary as well as functional significance of these repeats in SRY coding sequence is yet to be explored.
Many transcripts were found also for EIF3CY (85) and ZNF33bY (64). However, these numbers might be inflated because the coding sequences of the autosomal and Y-derived homologs of the two genes were identical, and it was not possible to design RT-PCR primers to clearly distinguish between the autosomal and Y-derived cDNA amplicons.
Transcriptional profiles of the 37 MSY genes were studied on a panel of nine adult equine body tissues by reverse transcriptase (RT) PCR (Fig. 3). The primers for two MSY genes, TBL1Y and STS-Y, did not show male-specific amplification and hence were removed from analysis, leaving results for 35 genes. Transcription profiles of these 35 MSY genes/transcripts were classified in three categories (Table 1):
- Ten genes (ETSTY1-6, RBMY, TSPY, UBE1Y, ZNF33bY), all present in multiple copies (according to cDNA FISH), were expressed exclusively in testis (Fig. 3a);
- Six genes (CUL4BY, ETY1, NLGN4Y, RFX5Y, SRY, YIR2) showed intermediate or broader expression, and were transcribed in testis and in a few other tissues. Notably, SRY and YIR2 expression was predominant in testis (Fig. 3b);
- Sixteen genes (ATPV06CY, CYorf15, DDX3Y, EIF1AY, EIF2s3Y, EIF3CY, ETY2, ETY4, MT-ND1Y, MAP3K7IP3Y, RPS3AY, KDM5D, TMSB4Y, USP9Y, UTY, ZFY) were expressed ubiquitously in all nine tissues studied (Fig. 3c).
a. Testis-specific expression. b. Intermediate expression; c. Ubiquitous expression (ACTB was used as an internal control). Lanes: M - molecular markers (100 bp ladder, New England Biolabs); 1 - brain, 2 - kidney, 3 - heart, 4 – skeletal muscle, 5 - liver, 6 - lung, 7 - spleen, 8 - seminal vesicle, 9 - testis, 10 - no mRNA control, 11 - no RT control, 12 - no genomic DNA control, 13 - male genomic DNA control, 14 - female genomic DNA control.
The remaining three genes, AMELY, KAL1Y and ETY3, were not expressed in any of the 9 tissues. The results for AMELY were expected because the gene is known to be expressed exclusively in developing tooth buds , while KAL1 might be a Y-linked pseudogene as it is in humans . Transcriptional status of ETY3, however, remained tentative and will be ascertained in future studies using a more comprehensive panel of male equine adult and embryonic tissues. Until then, the marker is named as E(T)Y3.Testis-specific ZNF33bY and testis predominant YIR2 produced several different RT-PCR amplicons (Fig. 3a, b) indicating that the multiple copies of the two genes give rise to many different transcripts in the horse tissues analyzed. The functional significance of multiple transcripts can be addressed in future studies.
Finally, it must be noted that since the tissue panel comprised of selected adult tissues, the specific expression profiles as determined in this study do not necessarily represent the functional profiles of the horse MSY genes throughout the development.
Full-length cDNA sequences of multicopy, testis-specific MSY genes
Multicopy MSY genes with testis-specific expression are most frequently involved in male fertility related functions in human and mouse , . Therefore, bearing in mind their possible role in stallion fertility, full length cDNA sequences were obtained for TSPY (1,037 bp), ETSTY2 (2,323 bp) and ETSTY5 (1,635 bp). The length of horse TSPY cDNA is quite similar to human (1,160 bp; NM_003308) but half the size of the domestic cat (2,089 bp; clone a244, DQ329518) TSPY. Despite the size differences, the horse TSPY cDNA shares 78% sequence similarity with 522 nucleotides (nt) in human, and 75% similarity with 577 nt in cat TSPY. Due to the uncertainly of the RACE procedure, despite several attempts, only partially extended cDNA sequences were generated for ETSTY1, 3, 4, 6, and RBMY, while no additional sequence was obtained for UBE1Y and ZNF33bY. Novel transcripts ETSTY1, 3, 4 and 6 did not align with each other while using standard stringent assembly parameters in Sequencher program. When the sequence assembly parameters were relaxed, the three sequences did show some overlap. Thus, it is possible that they are members of a gene family which will be confirmed when the full sequence of the Y chromosome is available. The generation of full-length cDNA sequences was important to look for their protein coding ability and hence likely involvement in male fertility.
Protein coding potential of MSY genes
To investigate how many MSY genes might have a protein coding potential, all cDNA sequences were analyzed for the presence of open reading frames (ORFs). We found ORFs in 18 horse MSY genes (Table 1). Among these, TSPY cDNA has eight ORFs of which the longest is 807 nucleotides. The remaining seven ORFs are present at different overlapping regions of the longest ORF and are 693 bp, 375 bp, 366 bp, 342 bp, 330 bp, 276 bp and 237 bp, respectively. All seven ORFs, except the shortest, encode TSPY protein of 269, 231, 125, 122, 114, 110 and 92 amino acids, respectively. The protein contains a NAP (nucleosome assembly protein) domain which is conserved across mammalian species, and is important for a diverse spectrum of cellular and molecular functions . It is thus likely that TSPY protein can be important for male fertility related function in horses. Notably, ORFs were found in several novel, as yet equine-specific transcripts (Table 1) including the full length cDNA of ETSTY2 and ETSTY5. The corresponding hypothetical proteins, however, showed no homology to known mammalian protein sequences. It is possible that novel equine transcripts encode novel proteins. Alternatively, these novel, as yet equine-specific amplified sequences, similarly to many ampliconic MSY sequences in human and chimpanzee , , might be non-coding transcription units.
Comparative analysis of horse MSY genes in the donkey
There are striking differences in MSY gene content between some closely related species, such as human and chimpanzee  but not between others, like human and gorilla . To investigate Y chromosome evolution in equids, we compared horse MSY gene content with that of the donkey (Equus asinus). Evolutionary distance between the two species is about 3 million years –, thus half the time that separates human and chimpanzee .
We attempted PCR with the 37 horse MSY gene primers in donkey genomic DNA (Fig. S2), and showed that 29 genes, including 6 novel testis-specific transcripts (ETSTY1-6), are shared between the two equid Y chromosomes. Five genes (DDX3Y, E(T)Y3, MAP3K7IP3Y, NLGN4Y, UBE1Y) amplified in male and female donkeys, though in horses the same primers were male specific. Therefore, we were unable to confirm Y-specific nature of these sequences in donkeys. Similarly to the horse, it was not possible to distinguish by PCR between STS-Y/X and TBL1Y/X in donkeys. Remarkably, donkey amplicons of E(T)Y3 and ZNF33bY were of different sizes compared to horse (Fig. S2), and ETY2 and EIFCY (smaller male-specific sequence) were not found in the donkey.
Next, we determined expression profiles of the 15 horse MSY genes, including all novel transcripts, five acquired sequences, and KDM5D as an X-degenerate control, in donkey testis (Fig. 4). All genes, except E(T)Y3 and ETSTY2, were expressed in donkey and sequence identity between the homologous horse and donkey transcripts was 95-100% (data not shown). These data demonstrate that overall, the horse and donkey Y chromosomes are similar in DNA sequence and gene content, but show also species specific structural and transcriptional differences. More studies, including gene copy number analysis and detailed expression profiling of all asine MSY genes, will determine the homology between the two Y chromosomes in more detail.
1 - horse testis (positive control); 2–3 – testis of two different donkeys; 4 - no mRNA control; 5 - male horse genomic DNA control; 6 - male donkey genomic DNA control; M - molecular markers (100 bp ladder, New England Biolabs). ACTB was used as an internal control.
Comparison of horse MSY with other mammalian Y chromosomes
The two broad categories of mammalian MSY genes, X-degenerate and ampliconic are present in human , chimpanzee , gorilla , mouse , , cat –, and cattle . Additionally, Y chromosomes have frequently acquired sequences by transposition or retroposition from other parts of the genome . In this study, we demonstrated that X-degenerate, ampliconic and acquired sequences are also present in the horse MSY (Table 2).
The X-degenerate genes originate from mammalian proto-sex chromosomes and have been retained as gametologues on both the X and the Y chromosome , , –. They are typically orthologous between species, and are truly the only comparative loci across mammalian Y chromosomes. Indeed, almost two thirds of the 20 X-degenerate genes found in horse have a Y-linked homologue in primates , , , –, carnivores –, rodents ,  or other mammalian species –, ,  (Table 2). Not surprisingly, all 20 genes are present in the donkey as well.
Like in other species , –, the majority of horse X-degenerate genes are single copy sequences with broad or ubiquitous expression profiles (Table 1). A few X-degenerate genes, viz., CUL4BY, RBMY, TSPY and UBE1Y, have been amplified and have acquired testis-specific or testis predominant expression (Table 1, Fig. 3a, b). Notably, RBMY is a multicopy testis-specific transcript in human, mouse and cat , , , and TSPY is a multicopy and testis-specific gene in most species studied so far , , –. The only known exception is mouse where TSPY has become a single copy pseudogene . In comparison, more evolutionary changes have shaped UBE1Y. Horse is the only species where UBE1Y is both multicopy and with testis-specific expression (Table 1, Fig. 1a). Orthologs in other species (cat, pig, mouse) are single copy , , , and only murine UBE1Y is expressed exclusively in testis . The gene has altogether been lost from human MSY , . In mouse Y chromosome, UBE1Y is located in a region encoding the spermatogenesis factor, Spy, which is required for the normal proliferation of germ cells. Due to its testis-specific expression , the UBE1Y has been considered as a possible candidate gene regulating germ cell proliferation and, thus, male fertility. Given the high copy number and testis-specific transcription of UBE1Y in horses, we hypothesize that the gene has acquired functions that are specifically associated with ubiquitin activation and protein turnover  in equine germ cells. The high number of UBE1Y cDNAs recovered by direct selection (Table 1) also signifies the functional importance of these transcripts in horse testis.
An interesting finding was the presence of an equine Y-linked cullin 4B (CUL4BY) orthologue. This highly amplified and testis-specific transcript has as yet been identified in the cats and dogs –. The equine CUL4BY is also multicopy as in the two carnivores but has a broader expression profile being transcribed in testis, heart and kidney (Table 1). We speculate that the horse CUL4BY might be in a transitional stage towards restricting its E3 ubiquitin ligase functions  specifically to testis and male germ cell proliferation.
The most intriguing X-degenerate gene, however, is SRY. Considering the known function of SRY in sex determination at early stages of mammalian embryonic development , it comes as a surprise that the single copy SRY is transcribed at high levels in adult horse testis (Table 1). Functional importance of SRY in adult males is not yet known. However, the presence of multiple copies of SRY sequences in rabbit , rat  and cat  Y chromosomes further supports our assumption that SRY has more functions than sex determination, and the transcripts might be actively needed also in mature testis.
Ampliconic sequences are defined as amplified or multiple segments of euchromatic sequences that exhibit as much as 99.9% identity over 10–100 kilobasepairs with other MSY sequences, and comprise multicopy gene families . These regions have been sequenced only in human  and chimpanzee  though multicopy MSY gene families have been found also in other primates , mouse –, carnivores , horse  and cattle . The evolutionary origin of these sequences is diverse. Some genes, like TSPY or RBMY, originate from ancestral X-degenerate genes (discussed above). Others, like human DAZ and CDY , –, mouse RhoA , cat FLJ36031Y , or horse ZNF33bY (this study) have been transposed and amplified from autosomes. Several ampliconic sequences, however, are Y-borne and show no sequence similarity between distantly related species . Similarly, the horse MSY has acquired 10 novel amplified and expressed sequences that are partly shared with the donkey Y chromosome (in sequence homology and transcriptional status), but not with any other mammalian species studied so far (Table 2).
Unexpectedly, three horse cDNA sequences, viz., E(T)Y3, ETSTY6 and ZNF33bY mapped to the ECAY heterochromatin. Two transcripts, ETSTY6 and ZNF33bY, are expressed exclusively in testis (Table 1), and an ORF was found in the partially extended cDNA sequence of ETSTY6. To the best of our knowledge this is the second report that highly amplified transcripts with protein coding potential are found in mammalian Y chromosome heterochromatin. Previously, a 450 kb euchromatic island was identified in the pericentromeric heterochromation of the human Y chromosome . Similarly to the horse, the region in humans is highly duplicated, and contains genes with ORFs including members of the homeobox gene family DUX. Most likely, these sequences in horse have been highly amplified to carry out important testicular function and are therefore expressed exclusively in testis.
Species-specific features were observed also in the distribution of ampliconic sequences in MSY. In horse, multicopy sequences are localized in relatively small regions in Contig I and the heterochromatin (Fig. 2) comprising two distinct blocks on ECAYq. Human amplicons are distributed between at least 5 distinct regions along HSAY , while in chimpanzee they are consolidated into two main blocks on Yp and proximal Yq . In other species, such as mouse , cat  and pig (our unpublished data), tandemly repeated ampliconic arrays comprised of multicopy gene families are dispersed over the entire long arm of the Y chromosome. For example, some mouse testis-specific gene families (Ssty, Asty) each are present in as many as 65–100 copies , ,  Thus, despite substantial differences, the architecture of horse MSY resembles more that of primates than other mammals studied so far.
Contrasting these structural differences in MSY multicopy gene families, functional features of these sequences tend to be more similar in different species. Like in human, mouse and cat , , , , most equine Y-borne amplified sequences are expressed exclusively or predominantly in testis (Table 1), and have presumably a role in testicular functions. Substantial evidences for this have been provided by human and mouse Y chromosome studies –, , . It has also been argued that gene amplification on sex chromosomes might be needed for rapid compensation for sex chromatin repression after male meiosis , . Taken together, the multicopy portion of mammalian MSYs may share very little direct sequence homology between species, but is surprisingly consistent in function. Thus, the 10 multicopy and testis-specific genes identified in this study (Table 1) are the primary candidate genes for stallion fertility, and subject for future studies.
Acquisition of sequences from autosomes, the X-chromosome or mitochondrial genome is another characteristic feature of Y chromosome evolution , . Such transposed or retrotransposed sequences can acquire testis-restricted functions, and are frequently amplified. The examples are human DAZ  or mouse RhoA  gene families. Other transposed sequences might lose their original functions and be retained as pseudogenes , . Structural, functional, and evolutionary analyses of the three bovid Y specific gene families – also support the idea that the Y chromosome tends to acquire and amplify fertility related genes or even blocks of genes from other genomic regions . Detailed molecular and functional analysis of bovine DDX3Y and it's X-linked and autosomal homologs show that the bDDX3 gene family is expressed predominantly in testis and brain, thus being a good candidate to be involved in spermatogenesis . Such gene traffic, however, is species or group specific because in different species different autosomal genes have acquired Y-linked counterparts. This is consistent with the results of the present study showing that none of the autosome derived genes on horse MSY have, as yet, been found Y-linked in other mammals (Table 2). Among the six acquired genes on the horse MSY, only ZNF33bY, a member of zinc finger protein families, has been amplified and become testis-specific. Likewise, two zinc finger genes, ZNF280BY and ZNF280AY, have been acquired by the cattle Y chromosome . Similarly to the equine ZNF33bY, the cattle ZNF280BY is predominantly expressed in testis and possible involvement of this gene in spermatogenesis has been suggested . The remaining five acquired equine MSY genes were single copy genes with intermediate or ubiquitous expression, and their functional importance has yet to be determined. We hypothesize that the intermediately expressed RFX5Y might be associated with minor histocompatibility complex and H–Y antigens  because the autosomal RFX5 protein is a part of conserved transcriptional coactivator complex binding to the MHC-II promoters . The ubiquitously expressed male specific homolog of mitochondrial MT-ND1 is probably the first NUMT (nuclear sequences of mitochondrial origin) found in the horse MSY. Though limited data are available for NUMTs in the horse genome , human studies show that the Y chromosome is more susceptible for mtDNA insertions than the rest of the genome . Next, given the known role of the autosomal ATP6V0C, a component of a multi-subunit membrane transporter, in regulating sperm motility and maturation in humans , it is possible that the equine Y-linked ATP6V0CY has acquired a similar function. Finally, we could not clearly determine the transcriptional status of EIF3CY and RPS3AY because the RT-PCR amplicons of Y-linked and autosomal homologs were the same size. Therefore it is likely that EIF3CY and RPS3AY are pseudogenes and the recovered transcripts originate from their autosomal counterparts. Overall, we conclude that gene acquisition on the Y chromosome is a species or lineage specific event, and the presence of equine acquired Y-linked sequences was observed only in the donkey but not in other mammals studied to date (Table 2).
We have demonstrated that ECAY, like the Y chromosomes in primates, mouse, cattle and cat, is comprised of functional genes and expressed sequences. About half of horse MSY genes are shared with other mammals and are of ancestral origin. The remaining genes on the MSY comprise novel Y-borne or transposed genes which, according to the current comparative information, are horse and/or donkey specific. All Y-borne novel sequences and ZNF33bY have been amplified and became multicopy on ECAY. The horse MSY gene catalogue is the first detailed information of a Y chromosome in a perissodactyl species, thus improving our knowledge about the evolutionary processes that have shaped Y chromosomes in different eutherian lineages. We infer that despite substantial differences in the organization of mammalian Y chromosomes, the likely functions of several MSY genes and transcripts might be conserved and are related predominantly to testis and possibly to male fertility. These findings establish an important foundation for the study of Y-linked genetic factors governing fertility in stallions. Finally, since the genome sequencing project of the domestic horse used DNA from a female animal, the MSY gene catalogue is the first extensive collection of male specific genes and sequences in horses.
Materials and Methods
Procurement of equine and asine tissues was performed according to the United States Government Principles for the Utilization and Care of Vertebrate Animals Used in Testing, Research and Training and were approved by the Clinical Research Review Committee (CRRC #08-33) at Texas A&M University.
DNA samples and chromosome preparations
Genomic DNA of five normal male and female horses, and one female and two male donkeys was isolated from peripheral blood using standard protocols . Equine chromosome preparations were obtained from blood lymphocytes of a male horse following our protocol .
Selection of Y-specific cDNA sequences
Two rounds of direct cDNA selection were carried out as described earlier . We used normal adult horse testis cDNA as the driver, and horse Y chromosome sequences as selectors. Testis mRNA was isolated using Fast Track 2.0 mRNA isolation kit (Invitrogen), and cDNA was synthesized using random primers and Superscript II reverse transcriptase (Invitrogen). The cDNA was adapter-ligated and amplified by PCR. Horse Y chromosome specific selectors were generated from: i) 7000 copies of flow sorted and GenomiPhi (Amersham Biosciences) amplified horse Y chromosome (provided by Cambridge Resource Centre for Comparative Genomics), and ii) 183 BAC clones from the horse MSY contig map  (Fig. 2, Table S2). The BAC clones were divided into 6 pools with ∼30 clones in each. Amplified testis cDNA was annealed with horse Cot-1 DNA (cDNA/Cot-1 ratio ranged from 1∶2 to 1∶7.5) for 4 h to block repetitive sequences. In some experiments, we added UBE1Y cDNA to Cot-1 DNA to conceal this most abundant Y-specific transcript, and facilitate the discovery of unique expressed sequences. Flow sorted ECAY and the 6 MSY BAC pools were labeled with biotin according to our protocol , and hybridized individually with pre-annealed testis cDNA for 50 h. Testis cDNA and biotinylated Y chromosome hybrids were selected with streptavidin coated paramagnetic beads (Dynabeads® M-280 Streptavidin, Invitrogen) and Y-specific testis cDNA was eluted. This primary selected cDNA was amplified by PCR and used for a second round of hybridization as described above.
Cloning, sequencing and analysis of cDNA sequences
Selected cDNA from the second round of hybridization was amplified by PCR and cloned en masse into TOPO-TA cloning vector (Invitrogen). Plasmid clones were picked and grown overnight at 37°C in 96-well culture plates containing LB media and ampicillin (50 µg/ml). Plasmid DNA was isolated by alkaline lysis using REAL-prep96 kit (Qiagen). Randomly selected 152 plasmid clones were digested with EcoRI (Invitrogen), and analyzed on 2% agarose gels for the presence of inserts. The cDNA clones were sequenced using BigDye (Applied Biosystems) terminator chemistry, universal primers and ∼300-500 ng of plasmid DNA as a template. The sequencing reactions were resolved on an ABI-3730 capillary sequencer (Applied Biosystems). Sequences were quality trimmed and assembled into contigs using Sequencher V 4.7 software (GeneCodes Co). The contigs were checked for repetitive elements with RepeatMasker (http://www.repeatmasker.org) and analyzed using Discontiguous MegaBLAST (http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi; cut-off threshold 1e-8) to identify putative orthologs in human, mouse and other mammalian genomes. The sequences were aligned with WG sequence assembly EquCab2 of a female horse (http://www.ensembl.org/Equus_caballus/index.html), and likely male-specific cDNAs were subtracted. Male specificity of the cDNA sequences was further validated by PCR by using cDNA-specific primers (see below) on male and female genomic DNAs. Exon-intron boundaries of the partial cDNA sequences were tentatively determined using orthologous sequences of mammalian Y-linked genes (http://www.ensembl.org/index.html), and exonic primers for PCR (Table S1) were designed using Primer 3 software (http://frodo.wi.mit.edu/primer3/input.htm). Where possible, the primers were designed in neighboring exons to span an intron. In most cases, however, intron spanning primers could not be designed because of limited sequence information and knowledge about the gene structure. All PCR reactions were carried out in 10 µl volume containing 1X PCR buffer (Sigma Aldrich), 0.3 pmol of each primer, 0.2 mM dNTPs, 1.5 mM MgCl2, 0.25 units JumpStart REDTaq DNA polymerase (Sigma Aldrich), and 50 ng of genomic DNA. Each primer pair was amplified from the genomic DNA of 5 normal male and 5 normal female horses, and from one female and two male donkeys. The PCR products were stained with ethidium bromide and resolved on 2.0 % agarose gels.
Sequence tagged site (STS) content and BAC end sequence analysis
Male specific cDNA sequences were assigned to MSY contig map by STS content analysis. The presence or absence of cDNA sequences in MSY BAC clones was determined by PCR using exonic cDNA primers and DNA from each of the 183 MSY BAC clones. If a male specific cDNA sequence was not found in the MSY contig map, the cDNA primers were used to screen the CHORI-241 library by PCR. New BAC clones were isolated as described by us earlier . BAC end sequences were retrieved from NCBI (http://www.ncbi.nlm.nih.gov/) Entrez Nucleotide and analyzed by NCBI BLAST. Additionally, 44 anonymous male-female specific cDNA sequences were PCR amplified on the 12 BAC clones that form the minimum tiling path of the horse PAR . One gene, ATP6V0CY, was discovered by analyzing the whole sequence of the BAC 107.3H9 .
Fluorescence in situ hybridization (FISH)
We used FISH to check the Y-specificity of flow sorted ECAY, the 6 MSY BAC pools, all newly isolated BAC clones, and to evaluate copy numbers of male specific cDNA sequences. Probe labeling, in situ hybridization, signal detection and image analysis were carried out according to our detailed protocol .
Tissue collection, RNA isolation and reverse transcriptase (RT)-PCR
Fresh necropsy samples of normal adult male horse tissues, viz., brain, kidney, heart, skeletal muscle, liver, lungs, spleen, seminal vesicle, and testes, were collected in RNA-later (Ambion). Likewise, testis tissue was obtained by castration from two male donkeys. Tissue specific mRNA was extracted with FastTrack 2.0 Kit (Invitrogen). If it was possible to predict the gene structure and exon-intron boundaries, intron-spanning primers were designed from neighboring exons for RT-PCR. Otherwise, and in cases where introns were very large, exonic primers (see above) were used. Primers and PCR conditions are listed in Table S1. RT-PCR reactions were carried out in 15 µl volume using Superscript III One-Step RT-PCR System and Platinum Taq DNA polymerase (Invitrogen), 40 pmol of each primer and 10 ng of mRNA. The cycling conditions were as follows: 30 min at 50°C, 2 min at 94°C, 30 cycles of 15 s at 94°C, 30 s at 58°C, 1 min at 68°C, and final extension at 68°C for 5 min. Genomic controls were run simultaneously with the mRNA samples and RT-PCR products were visualized on 2.0% agarose gels. In order to compare the gene expression among the selected tissues, a housekeeping gene ACTB was used as an internal control.
Generation of full-length cDNA using 3′ and 5′ RACE
Rapid Amplification of cDNA Ends (RACE) was performed separately for 3′ and 5′ ends using GeneRacerTA kit (Invitrogen) and 1 µg of testis total RNA. For the 5′ end of each partial cDNA sequence, one 5′ reverse primer and one 5′ nested reverse primer were designed using Primer 3 software (http://frodo.wi.mit.edu/primer3/input.htm) in conjunction with GeneRacerTA 5′ forward and 5′ nested forward primers, respectively (Table S3). Similarly, 3′ forward primer and one 3′ nested forward primer were designed in conjunction with GeneRacerTA 3′ reverse and 3′ nested reverse primers, respectively (Table S3). In both cases, manufacturer's instructions were followed. The first round PCR cycling conditions for RACE were: hot-start at 94°C for 2 min; 5 cycles of 94°C for 30 s, 72°C for 1 min; 5 cycles of 94°C for 30 s, 70°C for 1 min; 20 cycles of 94°C for 30 s, 65°C for 30 s, and 68°C for 1 min. A final extension of 10 min at 68°C completed the reaction. The PCR product obtained from the first round of amplification was diluted 10 times and 1 µl of the dilution was used as a template for nested RACE PCR with nested primers. Nested RACE PCR cycling conditions were as follows: hot-start at 94°C for 2 min; 20 cycles of 94°C for 30 s, 65°C for 30 s, and 68°C for 2 min, followed by final extension of 10 min at 68°C. Products of nested RACE PCR were resolved on a 2% agarose gel, the bands were cut, and PCR products were eluted using S.N.A.P. columns provided with the kit. PCR products were cloned using TOPO TA Cloning Kit for Sequencing (Invitrogen). Transformed cells were plated on LB agar containing 50 µg/ml of ampicillin. Colonies were picked after an overnight incubation at 37°C and cultured overnight at 37°C in LB medium with 50 µg/ml ampicillin. Plasmid DNA was extracted using REAL Prep 96-well Kit (Qiagen), and sequenced with universal primers as described above.
Open Reading frames and potential proteins
All cDNA sequences, full-length and partial, were analyzed for the presence of open reading frames (ORFs) using Sequencher V 4.7 (GeneCodes Co) and NCBI ORF finder (http://www.ncbi.nlm.nih.gov/projects/gorf/) softwares packages. The potential protein sequences were compared with the available protein database using NCBI protein BLAST algorithm BLASTP (http://blast.ncbi.nlm.nih.gov/Blast.cgi). Additionally, NCBI conserved domain database search (http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi) was used to identify the presence of any conserved domain in the putative protein sequences.
Amplification by PCR (30 cycles) of EIF3CY (lanes 1–2), RPS3AY (lanes 3–4) and ZNF33bY (lanes 5–6) from male (odd numbered lanes) and female (even numbered lanes) horse genomic DNA. Note that the lower bands are present only in males. M1 - molecular markers (100 bp ladder, New England Biolabs), M2 (1kb ladder, New England Biolabs).
Comparative amplification by PCR (30 cycles) of horse MSY genes from horse and donkey genomic DNA. 1 – male horse; 2–3 – two male donkeys; 4- female donkey; M - molecular markers (100 bp ladder, New England Biolabs).
In formation about horse MSY genes, PCR primers, and sequences
List of BAC clones used for cDNA selection
Conceived and designed the experiments: BPC TR WJM. Performed the experiments: NP TR AJPW. Analyzed the data: NP TR WJM. Contributed reagents/materials/analysis tools: PCMO MAF-S CCL CA PR. Wrote the paper: TR NP BPC WJM.
- 1. Skaletsky H, Kuroda-Kawaguchi T, Minx PJ, Cordum HS, Hillier L, et al. (2003) The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes. Nature 423: 825–837.H. SkaletskyT. Kuroda-KawaguchiPJ MinxHS CordumL. Hillier2003The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes.Nature423825837
- 2. Quintana-Murci L, Fellous M (2001) The Human Y Chromosome: The Biological Role of a "Functional Wasteland". J Biomed Biotechnol 1: 18–24.L. Quintana-MurciM. Fellous2001The Human Y Chromosome: The Biological Role of a "Functional Wasteland".J Biomed Biotechnol11824
- 3. Graves JA (2006) Sex chromosome specialization and degeneration in mammals. Cell 124: 901–914.JA Graves2006Sex chromosome specialization and degeneration in mammals.Cell124901914
- 4. Hughes JF, Skaletsky H, Pyntikova T, Graves TA, van Daalen SK, et al. (2010) Chimpanzee and human Y chromosomes are remarkably divergent in structure and gene content. Nature 463: 536–539.JF HughesH. SkaletskyT. PyntikovaTA GravesSK van Daalen2010Chimpanzee and human Y chromosomes are remarkably divergent in structure and gene content.Nature463536539
- 5. Grzmil P, Golas A, Muller C, Styrna J (2007) The influence of the deletion on the long arm of the Y chromosome on sperm motility in mice. Theriogenology 67: 760–766.P. GrzmilA. GolasC. MullerJ. Styrna2007The influence of the deletion on the long arm of the Y chromosome on sperm motility in mice.Theriogenology67760766
- 6. McElreavey K, Mitchell MJ (2002) The sexually transmitted genome. Trends Genet 18: 13–14.K. McElreaveyMJ Mitchell2002The sexually transmitted genome.Trends Genet181314
- 7. Toure A, Grigoriev V, Mahadevaiah SK, Rattigan A, Ojarikre OA, et al. (2004) A protein encoded by a member of the multicopy Ssty gene family located on the long arm of the mouse Y chromosome is expressed during sperm development. Genomics 83: 140–147.A. ToureV. GrigorievSK MahadevaiahA. RattiganOA Ojarikre2004A protein encoded by a member of the multicopy Ssty gene family located on the long arm of the mouse Y chromosome is expressed during sperm development.Genomics83140147
- 8. Toure A, Szot M, Mahadevaiah SK, Rattigan A, Ojarikre OA, et al. (2004) A new deletion of the mouse Y chromosome long arm associated with the loss of Ssty expression, abnormal sperm development and sterility. Genetics 166: 901–912.A. ToureM. SzotSK MahadevaiahA. RattiganOA Ojarikre2004A new deletion of the mouse Y chromosome long arm associated with the loss of Ssty expression, abnormal sperm development and sterility.Genetics166901912
- 9. Toure A, Clemente EJ, Ellis P, Mahadevaiah SK, Ojarikre OA, et al. (2005) Identification of novel Y chromosome encoded transcripts by testis transcriptome analysis of mice with deletions of the Y chromosome long arm. Genome Biol 6: R102.A. ToureEJ ClementeP. EllisSK MahadevaiahOA Ojarikre2005Identification of novel Y chromosome encoded transcripts by testis transcriptome analysis of mice with deletions of the Y chromosome long arm.Genome Biol6R102
- 10. Ellis PJ, Ferguson L, Clemente EJ, Affara NA (2007) Bidirectional transcription of a novel chimeric gene mapping to mouse chromosome Yq. BMC Evol Biol 7: 171.PJ EllisL. FergusonEJ ClementeNA Affara2007Bidirectional transcription of a novel chimeric gene mapping to mouse chromosome Yq.BMC Evol Biol7171
- 11. Murphy WJ, Pearks Wilkerson AJ, Raudsepp T, Agarwala R, Schaffer AA, et al. (2006) Novel gene acquisition on carnivore Y chromosomes. PLoS Genet 2: e43.WJ MurphyAJ Pearks WilkersonT. RaudseppR. AgarwalaAA Schaffer2006Novel gene acquisition on carnivore Y chromosomes.PLoS Genet2e43
- 12. Pearks Wilkerson AJ, Raudsepp T, Graves T, Albracht D, Warren W, et al. (2008) Gene discovery and comparative analysis of X-degenerate genes from the domestic cat Y chromosome. Genomics 92: 329–338.AJ Pearks WilkersonT. RaudseppT. GravesD. AlbrachtW. Warren2008Gene discovery and comparative analysis of X-degenerate genes from the domestic cat Y chromosome.Genomics92329338
- 13. Liu WS, Mariani P, Beattie CW, Alexander LJ, Ponce De Leon FA (2002) A radiation hybrid map for the bovine Y Chromosome. Mamm Genome 13: 320–326.WS LiuP. MarianiCW BeattieLJ AlexanderFA Ponce De Leon2002A radiation hybrid map for the bovine Y Chromosome.Mamm Genome13320326
- 14. Liu WS, de Leon FA (2004) Assignment of SRY, ANT3, and CSF2RA to the bovine Y chromosome by FISH and RH mapping. Anim Biotechnol 15: 103–109.WS LiuFA de Leon2004Assignment of SRY, ANT3, and CSF2RA to the bovine Y chromosome by FISH and RH mapping.Anim Biotechnol15103109
- 15. Chang TC, Yang Y, Yasue H, Bharti AK, Retzel EF, et al. (2011) The expansion of the PRAME gene family in Eutheria. PLoS One 6: e16867.TC ChangY. YangH. YasueAK BhartiEF Retzel2011The expansion of the PRAME gene family in Eutheria.PLoS One6e16867
- 16. Yang Y, Chang TC, Yasue H, Bharti AK, Retzel EF, et al. (2011) ZNF280BY and ZNF280AY: autosome derived Y-chromosome gene families in Bovidae. BMC Genomics 12: 13.Y. YangTC ChangH. YasueAK BhartiEF Retzel2011ZNF280BY and ZNF280AY: autosome derived Y-chromosome gene families in Bovidae.BMC Genomics1213
- 17. Stafuzza NB, Abbassi H, Grant JR, Rodrigues-Filho EA, Ianella P, et al. (2009) Comparative RH maps of the river buffalo and bovine Y chromosomes. Cytogenet Genome Res 126: 132–138.NB StafuzzaH. AbbassiJR GrantEA Rodrigues-FilhoP. Ianella2009Comparative RH maps of the river buffalo and bovine Y chromosomes.Cytogenet Genome Res126132138
- 18. Ikawa T, Kakegawa A, Nagano T, Ando H, Yamakoshi Y, et al. (2005) Porcine amelogenin is expressed from the X and Y chromosomes. J Dent Res 84: 144–148.T. IkawaA. KakegawaT. NaganoH. AndoY. Yamakoshi2005Porcine amelogenin is expressed from the X and Y chromosomes.J Dent Res84144148
- 19. Quilter CR, Blott SC, Mileham AJ, Affara NA, Sargent CA, et al. (2002) A mapping and evolutionary study of porcine sex chromosome genes. Mamm Genome 13: 588–594.CR QuilterSC BlottAJ MilehamNA AffaraCA Sargent2002A mapping and evolutionary study of porcine sex chromosome genes.Mamm Genome13588594
- 20. Meadows JR, Hawken RJ, Kijas JW (2004) Nucleotide diversity on the ovine Y chromosome. Anim Genet 35: 379–385.JR MeadowsRJ HawkenJW Kijas2004Nucleotide diversity on the ovine Y chromosome.Anim Genet35379385
- 21. Meadows JR, Kijas JW (2009) Re-sequencing regions of the ovine Y chromosome in domestic and wild sheep reveals novel paternal haplotypes. Anim Genet 40: 119–123.JR MeadowsJW Kijas2009Re-sequencing regions of the ovine Y chromosome in domestic and wild sheep reveals novel paternal haplotypes.Anim Genet40119123
- 22. Geraldes A, Rambo T, Wing RA, Ferrand N, Nachman MW (2010) Extensive gene conversion drives the concerted evolution of paralogous copies of the SRY gene in European rabbits. Mol Biol Evol 27: 2437–2440.A. GeraldesT. RamboRA WingN. FerrandMW Nachman2010Extensive gene conversion drives the concerted evolution of paralogous copies of the SRY gene in European rabbits.Mol Biol Evol2724372440
- 23. Kirsch S, Weiss B, Miner TL, Waterston RH, Clark RA, et al. (2005) Interchromosomal segmental duplications of the pericentromeric region on the human Y chromosome. Genome Res 15: 195–204.S. KirschB. WeissTL MinerRH WaterstonRA Clark2005Interchromosomal segmental duplications of the pericentromeric region on the human Y chromosome.Genome Res15195204
- 24. Goto H, Peng L, Makova KD (2009) Evolution of X-degenerate Y chromosome genes in greater apes: conservation of gene content in human and gorilla, but not chimpanzee. J Mol Evol 68: 134–144.H. GotoL. PengKD Makova2009Evolution of X-degenerate Y chromosome genes in greater apes: conservation of gene content in human and gorilla, but not chimpanzee.J Mol Evol68134144
- 25. Reynard LN, Cocquet J, Burgoyne PS (2009) The multi-copy mouse gene Sycp3-like Y-linked (Sly) encodes an abundant spermatid protein that interacts with a histone acetyltransferase and an acrosomal protein. Biol Reprod 81: 250–257.LN ReynardJ. CocquetPS Burgoyne2009The multi-copy mouse gene Sycp3-like Y-linked (Sly) encodes an abundant spermatid protein that interacts with a histone acetyltransferase and an acrosomal protein.Biol Reprod81250257
- 26. Cocquet J, Ellis PJ, Yamauchi Y, Mahadevaiah SK, Affara NA, et al. (2009) The multicopy gene Sly represses the sex chromosomes in the male mouse germline after meiosis. PLoS Biol 7: e1000244.J. CocquetPJ EllisY. YamauchiSK MahadevaiahNA Affara2009The multicopy gene Sly represses the sex chromosomes in the male mouse germline after meiosis.PLoS Biol7e1000244
- 27. Ferguson L, Ellis PJ, Affara NA (2009) Two novel mouse genes mapped to chromosome Yp are expressed specifically in spermatids. Mamm Genome 20: 193–206.L. FergusonPJ EllisNA Affara2009Two novel mouse genes mapped to chromosome Yp are expressed specifically in spermatids.Mamm Genome20193206
- 28. Di Meo GP, Perucatti A, Floriot S, Incarnato D, Rullo R, et al. (2005) Chromosome evolution and improved cytogenetic maps of the Y chromosome in cattle, zebu, river buffalo, sheep and goat. Chromosome Res 13: 349–355.GP Di MeoA. PerucattiS. FloriotD. IncarnatoR. Rullo2005Chromosome evolution and improved cytogenetic maps of the Y chromosome in cattle, zebu, river buffalo, sheep and goat.Chromosome Res13349355
- 29. Humphray SJ, Scott CE, Clark R, Marron B, Bender C, et al. (2007) A high utility integrated map of the pig genome. Genome Biol 8: R139.SJ HumphrayCE ScottR. ClarkB. MarronC. Bender2007A high utility integrated map of the pig genome.Genome Biol8R139
- 30. Gvozdev VA, Kogan GL, Usakin LA (2005) The Y chromosome as a target for acquired and amplified genetic material in evolution. Bioessays 27: 1256–1262.VA GvozdevGL KoganLA Usakin2005The Y chromosome as a target for acquired and amplified genetic material in evolution.Bioessays2712561262
- 31. Manz E, Vogel T, Glatzel P, Schmidtke J (1998) Identification of an equine Y chromosome specific gene locus (eTSPY) with potential in preimplantation sex diagnosis. Theriogenology 49: 364.E. ManzT. VogelP. GlatzelJ. Schmidtke1998Identification of an equine Y chromosome specific gene locus (eTSPY) with potential in preimplantation sex diagnosis.Theriogenology49364
- 32. Senese C, Penedo MC, Shiue YL, Bowling AT, Millon LV (1999) A HaeIII PCR-RFLP in the ZFY/ZFX genes of horses. Anim Genet 30: 390–391.C. SeneseMC PenedoYL ShiueAT BowlingLV Millon1999A HaeIII PCR-RFLP in the ZFY/ZFX genes of horses.Anim Genet30390391
- 33. Hasegawa T, Sato F, Ishida N, Fukushima Y, Mukoyama H (2000) Sex determination by simultaneous amplification of equine SRY and amelogenin genes. Theriogenology 62: 1109–1110.T. HasegawaF. SatoN. IshidaY. FukushimaH. Mukoyama2000Sex determination by simultaneous amplification of equine SRY and amelogenin genes.Theriogenology6211091110
- 34. Hasegawa T, Ishida M, Harigaya T, Sato F, Ishida N, et al. (1999) Linear SRY transcript in equine testis. J Vet Med Sci 61: 97–100.T. HasegawaM. IshidaT. HarigayaF. SatoN. Ishida1999Linear SRY transcript in equine testis.J Vet Med Sci6197100
- 35. Shiue YL, Millon LV, Skow LC, Honeycutt D, Murray JD, et al. (2000) Synteny and regional marker order assignment of 26 type I and microsatellite markers to the horse X- and Y-chromosomes. Chrom Res 8: 45–55.YL ShiueLV MillonLC SkowD. HoneycuttJD Murray2000Synteny and regional marker order assignment of 26 type I and microsatellite markers to the horse X- and Y-chromosomes.Chrom Res84555
- 36. Hirota K, Piumi F, Sato F, Ishida N, Guerin G, et al. (2001) FISH assignment of two equine BAC clones containing SRY and ZFY. Anim Genet 32: 326–327.K. HirotaF. PiumiF. SatoN. IshidaG. Guerin2001FISH assignment of two equine BAC clones containing SRY and ZFY.Anim Genet32326327
- 37. Raudsepp T, Santani A, Wallner B, Kata SR, Ren C, et al. (2004) A detailed physical map of the horse Y chromosome. Proc Natl Acad Sci U S A 101: 9321–9326.T. RaudseppA. SantaniB. WallnerSR KataC. Ren2004A detailed physical map of the horse Y chromosome.Proc Natl Acad Sci U S A10193219326
- 38. Raudsepp T, Chowdhary BP (2008) The horse pseudoautosomal region (PAR): characterization and comparison with the human, chimp and mouse PARs. Cytogenet Genome Res 121: 102–109.T. RaudseppBP Chowdhary2008The horse pseudoautosomal region (PAR): characterization and comparison with the human, chimp and mouse PARs.Cytogenet Genome Res121102109
- 39. Lovett M, Kere J, Hinton LM (1991) Direct selection: a method for the isolation of cDNAs encoded by large genomic regions. Proc Natl Acad Sci U S A 88: 9628–9632.M. LovettJ. KereLM Hinton1991Direct selection: a method for the isolation of cDNAs encoded by large genomic regions.Proc Natl Acad Sci U S A8896289632
- 40. Lahn BT, Page DC (1997) Functional coherence of the human Y chromosome. Science 278: 675–680.BT LahnDC Page1997Functional coherence of the human Y chromosome.Science278675680
- 41. Makrinou E, Fox M, Lovett M, Haworth K, Cameron JM, et al. (2001) TTY2: a multicopy Y-linked gene family. Genome Res 11: 935–945.E. MakrinouM. FoxM. LovettK. HaworthJM Cameron2001TTY2: a multicopy Y-linked gene family.Genome Res11935945
- 42. Raudsepp T, Durkin K, Lear TL, Das PJ, Avila F, et al. (2010) Molecular heterogeneity of XY sex reversal in horses. Animal Genetics 41: 41–52.T. RaudseppK. DurkinTL LearPJ DasF. Avila2010Molecular heterogeneity of XY sex reversal in horses.Animal Genetics414152
- 43. Salido EC, Yen PH, Koprivnikar K, Yu LC, Shapiro LJ (1992) The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes. Am J Hum Genet 50: 303–316.EC SalidoPH YenK. KoprivnikarLC YuLJ Shapiro1992The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes.Am J Hum Genet50303316
- 44. Trask BJ, Allen S, Massa H, Fertitta A, Sachs R (1993) Studies of metaphase and interphase chromosomes using fluorescence in situ hybridization. Cold Spring Harb Symp Quant Biol. pp. 767–775.BJ TraskS. AllenH. MassaA. FertittaR. Sachs1993Studies of metaphase and interphase chromosomes using fluorescence in situ hybridization.Cold Spring Harb Symp Quant Biol767775
- 45. Trask BJ (2002) Human cytogenetics: 46 chromosomes, 46 years and counting. Nat Rev Genet 3: 769–778.BJ Trask2002Human cytogenetics: 46 chromosomes, 46 years and counting.Nat Rev Genet3769778
- 46. Ellis PJ, Affara NA (2006) Spermatogenesis and sex chromosome gene content: an evolutionary perspective. Hum Fertil (Camb) 9: 1–7.PJ EllisNA Affara2006Spermatogenesis and sex chromosome gene content: an evolutionary perspective.Hum Fertil (Camb)917
- 47. Oram SW, Liu XX, Lee TL, Chan WY, Lau YF (2006) TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells. BMC Cancer 6: 154.SW OramXX LiuTL LeeWY ChanYF Lau2006TSPY potentiates cell proliferation and tumorigenesis by promoting cell cycle progression in HeLa and NIH3T3 cells.BMC Cancer6154
- 48. Norman JE, Ashley MV (2000) Phylogenetics of Perissodactyla and tests of the molecular clock. J Mol Evol 50: 11–21.JE NormanMV Ashley2000Phylogenetics of Perissodactyla and tests of the molecular clock.J Mol Evol501121
- 49. Oakenfull EA, Lim HN, Ryder OA (2000) A survey of equid mitochondrial DNA: Implications for the evolution, genetic diversity and conservation of Equus. Conservation Genetics 1: 341–355.EA OakenfullHN LimOA Ryder2000A survey of equid mitochondrial DNA: Implications for the evolution, genetic diversity and conservation of Equus.Conservation Genetics1341355
- 50. Hughes JF, Skaletsky H, Pyntikova T, Minx PJ, Graves T, et al. (2005) Conservation of Y-linked genes during human evolution revealed by comparative sequencing in chimpanzee. Nature 437: 100–103.JF HughesH. SkaletskyT. PyntikovaPJ MinxT. Graves2005Conservation of Y-linked genes during human evolution revealed by comparative sequencing in chimpanzee.Nature437100103
- 51. Ellis PJ, Clemente EJ, Ball P, Toure A, Ferguson L, et al. (2005) Deletions on mouse Yq lead to upregulation of multiple X- and Y-linked transcripts in spermatids. Hum Mol Genet 14: 2705–2715.PJ EllisEJ ClementeP. BallA. ToureL. Ferguson2005Deletions on mouse Yq lead to upregulation of multiple X- and Y-linked transcripts in spermatids.Hum Mol Genet1427052715
- 52. Rohozinski J, Agoulnik AI, Boettger-Tong HL, Bishop CE (2002) Successful targeting of mouse Y chromosome genes using a site-directed insertion vector. Genesis 32: 1–7.J. RohozinskiAI AgoulnikHL Boettger-TongCE Bishop2002Successful targeting of mouse Y chromosome genes using a site-directed insertion vector.Genesis3217
- 53. Waters PD, Ruiz-Herrera A, Dobigny G, Garcia Caldes M, Robinson TJ (2007) Sex chromosomes of basal placental mammals. Chromosoma 116: 511–518.PD WatersA. Ruiz-HerreraG. DobignyM. Garcia CaldesTJ Robinson2007Sex chromosomes of basal placental mammals.Chromosoma116511518
- 54. Delbridge ML, Graves JA (2007) Origin and evolution of spermatogenesis genes on the human sex chromosomes. Soc Reprod Fertil: Suppl 651–17.ML DelbridgeJA Graves2007Origin and evolution of spermatogenesis genes on the human sex chromosomes.Soc ReprodFertilSuppl 65117
- 55. Glaser B, Grutzner F, Willmann U, Stanyon R, Arnold N, et al. (1998) Simian Y chromosomes: species-specific rearrangements of DAZ, RBM, and TSPY versus contiguity of PAR and SRY. Mamm Genome 9: 226–231.B. GlaserF. GrutznerU. WillmannR. StanyonN. Arnold1998Simian Y chromosomes: species-specific rearrangements of DAZ, RBM, and TSPY versus contiguity of PAR and SRY.Mamm Genome9226231
- 56. Kim HS, Takenaka O (1996) A comparison of TSPY genes from Y-chromosomal DNA of the great apes and humans: sequence, evolution, and phylogeny. Am J Phys Anthropol 100: 301–309.HS KimO. Takenaka1996A comparison of TSPY genes from Y-chromosomal DNA of the great apes and humans: sequence, evolution, and phylogeny.Am J Phys Anthropol100301309
- 57. Perry J, Palmer S, Gabriel A, Ashworth A (2001) A short pseudoautosomal region in laboratory mice. Genome Res 11: 1826–1832.J. PerryS. PalmerA. GabrielA. Ashworth2001A short pseudoautosomal region in laboratory mice.Genome Res1118261832
- 58. Das PJ, Chowdhary BP, Raudsepp T (2009) Characterization of the bovine pseudoautosomal region and comparison with sheep, goat, and other mammalian pseudoautosomal regions. Cytogenet Genome Res 126: 139–147.PJ DasBP ChowdharyT. Raudsepp2009Characterization of the bovine pseudoautosomal region and comparison with sheep, goat, and other mammalian pseudoautosomal regions.Cytogenet Genome Res126139147
- 59. Mazeyrat S, Saut N, Mattei MG, Mitchell MJ (1999) RBMY evolved on the Y chromosome from a ubiquitously transcribed X-Y identical gene. Nat Genet 22: 224–226.S. MazeyratN. SautMG MatteiMJ Mitchell1999RBMY evolved on the Y chromosome from a ubiquitously transcribed X-Y identical gene.Nat Genet22224226
- 60. Vogel T, Borgmann S, Dechend F, Hecht W, Schmidtke J (1997) Conserved Y-chromosomal location of TSPY in Bovidae. Chromosome Res 5: 182–185.T. VogelS. BorgmannF. DechendW. HechtJ. Schmidtke1997Conserved Y-chromosomal location of TSPY in Bovidae.Chromosome Res5182185
- 61. Dechend F, Schubert S, Nanda I, Vogel T, Schmid M, et al. (1998) Organization and expression of rat Tspy. Cytogenet Cell Genet 83: 270–274.F. DechendS. SchubertI. NandaT. VogelM. Schmid1998Organization and expression of rat Tspy.Cytogenet Cell Genet83270274
- 62. Schubert S, Dechend F, Skawran B, Kunze B, Winking H, et al. (2000) Silencing of the Y-chromosomal gene tspy during murine evolution. Mamm Genome 11: 288–291.S. SchubertF. DechendB. SkawranB. KunzeH. Winking2000Silencing of the Y-chromosomal gene tspy during murine evolution.Mamm Genome11288291
- 63. Mitchell MJ, Woods DR, Tucker PK, Opp JS, Bishop CE (1991) Homology of a candidate spermatogenic gene from the mouse Y chromosome to the ubiquitin-activating enzyme E1. Nature 354: 483–486.MJ MitchellDR WoodsPK TuckerJS OppCE Bishop1991Homology of a candidate spermatogenic gene from the mouse Y chromosome to the ubiquitin-activating enzyme E1.Nature354483486
- 64. Levy N, Navarro A, Bishop CE, Mitchell MJ (2000) The ubiquitin-activating enzyme E1 homologous genes on the mouse Y chromosome (Ube1y) represent one functional gene and six partial pseudogenes. Mamm Genome 11: 164–168.N. LevyA. NavarroCE BishopMJ Mitchell2000The ubiquitin-activating enzyme E1 homologous genes on the mouse Y chromosome (Ube1y) represent one functional gene and six partial pseudogenes.Mamm Genome11164168
- 65. Lahn BT, Pearson NM, Jegalian K (2001) The human Y chromosome, in the light of evolution. Nat Rev Genet 2: 207–216.BT LahnNM PearsonK. Jegalian2001The human Y chromosome, in the light of evolution.Nat Rev Genet2207216
- 66. Petroski MD, Deshaies RJ (2005) Function and regulation of cullin-RING ubiquitin ligases. Nat Rev Mol Cell Biol 6: 9–20.MD PetroskiRJ Deshaies2005Function and regulation of cullin-RING ubiquitin ligases.Nat Rev Mol Cell Biol6920
- 67. Wilhelm D, Palmer S, Koopman P (2007) Sex determination and gonadal development in mammals. Physiol Rev 87: 1–28.D. WilhelmS. PalmerP. Koopman2007Sex determination and gonadal development in mammals.Physiol Rev87128
- 68. Geraldes A, Ferrand N (2006) A 7-bp insertion in the 3′ untranslated region suggests the duplication and concerted evolution of the rabbit SRY gene. Genet Sel Evol 38: 313–320.A. GeraldesN. Ferrand2006A 7-bp insertion in the 3′ untranslated region suggests the duplication and concerted evolution of the rabbit SRY gene.Genet Sel Evol38313320
- 69. Turner ME, Martin C, Martins AS, Dunmire J, Farkas J, et al. (2007) Genomic and expression analysis of multiple Sry loci from a single Rattus norvegicus Y chromosome. BMC Genet 8: 11.ME TurnerC. MartinAS MartinsJ. DunmireJ. Farkas2007Genomic and expression analysis of multiple Sry loci from a single Rattus norvegicus Y chromosome.BMC Genet811
- 70. Saxena R, de Vries JW, Repping S, Alagappan RK, Skaletsky H, et al. (2000) Four DAZ genes in two clusters found in the AZFc region of the human Y chromosome. Genomics 67: 256–267.R. SaxenaJW de VriesS. ReppingRK AlagappanH. Skaletsky2000Four DAZ genes in two clusters found in the AZFc region of the human Y chromosome.Genomics67256267
- 71. Dorus S, Gilbert SL, Forster ML, Barndt RJ, Lahn BT (2003) The CDY-related gene family: coordinated evolution in copy number, expression profile and protein sequence. Hum Mol Genet 12: 1643–1650.S. DorusSL GilbertML ForsterRJ BarndtBT Lahn2003The CDY-related gene family: coordinated evolution in copy number, expression profile and protein sequence.Hum Mol Genet1216431650
- 72. Boettger-Tong HL, Agulnik AI, Ty TI, Bishop CE (1998) Transposition of RhoA to the murine Y chromosome. Genomics 49: 180–187.HL Boettger-TongAI AgulnikTI TyCE Bishop1998Transposition of RhoA to the murine Y chromosome.Genomics49180187
- 73. Mueller JL, Mahadevaiah SK, Park PJ, Warburton PE, Page DC, et al. (2008) The mouse X chromosome is enriched for multicopy testis genes showing postmeiotic expression. Nat Genet 40: 794–799.JL MuellerSK MahadevaiahPJ ParkPE WarburtonDC Page2008The mouse X chromosome is enriched for multicopy testis genes showing postmeiotic expression.Nat Genet40794799
- 74. Liu WS, Wang A, Yang Y, Chang TC, Landrito E, et al. (2009) Molecular characterization of the DDX3Y gene and its homologs in cattle. Cytogenet Genome Res 126: 318–328.WS LiuA. WangY. YangTC ChangE. Landrito2009Molecular characterization of the DDX3Y gene and its homologs in cattle.Cytogenet Genome Res126318328
- 75. Rosinski KV, Fujii N, Mito JK, Koo KKW, Xuereb SM, et al. (2008) DDX3Yencodes a class I MHC–restricted H-Y antigen that is expressed in leukemic stem cells. Blood 111: 4817–4826.KV RosinskiN. FujiiJK MitoKKW KooSM Xuereb2008DDX3Yencodes a class I MHC–restricted H-Y antigen that is expressed in leukemic stem cells.Blood11148174826
- 76. Lochamy J, Rogers EM, Boss JM (2007) CREB and phospho-CREB interact with RFX5 and CIITA to regulate MHC class II genes. Mol Immunol 44: 837–847.J. LochamyEM RogersJM Boss2007CREB and phospho-CREB interact with RFX5 and CIITA to regulate MHC class II genes.Mol Immunol44837847
- 77. Jiang F, Miao Y-W, Liu B, Shen X, ren J-F, et al. (2008) Mitochondrial introgressions into the nuclear genome of the domestic horse. Zoological Research 29: 577–584.F. JiangY-W MiaoB. LiuX. ShenJ-F ren2008Mitochondrial introgressions into the nuclear genome of the domestic horse.Zoological Research29577584
- 78. Ricchetti M, Tekaia F, Dujon B (2004) Continued colonization of the human genome by mitochondrial DNA. PLoS Biol 2: E273.M. RicchettiF. TekaiaB. Dujon2004Continued colonization of the human genome by mitochondrial DNA.PLoS Biol2E273
- 79. Jefferies KC, Cipriano DJ, Forgac M (2008) Function, structure and regulation of the vacuolar (H+)-ATPases. Arch Biochem Biophys 476: 33–42.KC JefferiesDJ CiprianoM. Forgac2008Function, structure and regulation of the vacuolar (H+)-ATPases.Arch Biochem Biophys4763342
- 80. Birren B, Green ED, Klapholz S, Myers RM, Roskams J (1997) Genome Analysis. Analyzing D N A Laboratory Manual. New York: Cold Spring Harbor Laboratory press. B. BirrenED GreenS. KlapholzRM MyersJ. Roskams1997Genome Analysis.Analyzing D N A Laboratory ManualNew YorkCold Spring Harbor Laboratory press
- 81. Raudsepp T, Chowdhary BP (2008) FISH for mapping single copy genes. In: Murphy W, editor. Phylogenomics: Methods in Molecular Biology. 310. New Jersey: Humana Press.. T. RaudseppBP Chowdhary2008FISH for mapping single copy genes. In: Murphy W, editor. Phylogenomics: Methods in Molecular Biology.310New Jersey: Humana Press.
- 82. Del Mastro RG, Lovett M (1997) Isolation of coding sequences from genomic regions using direct selection. Methods Mol Biol 68: 183–199.RG Del MastroM. Lovett1997Isolation of coding sequences from genomic regions using direct selection.Methods Mol Biol68183199
- 83. Chowdhary BP, Paria N, Raudsepp T (2008) Potential applications of equine genomics in dissecting diseases and fertility. Anim Reprod Sci 107: 208–218.BP ChowdharyN. PariaT. Raudsepp2008Potential applications of equine genomics in dissecting diseases and fertility.Anim Reprod Sci107208218
- 84. Agulnik AI, Mitchell MJ, Lerner JL, Woods DR, Bishop CE (1994) A mouse Y chromosome gene encoded by a region essential for spermatogenesis and expression of male-specific minor histocompatibility antigens. Hum Mol Genet 3: 873–878.AI AgulnikMJ MitchellJL LernerDR WoodsCE Bishop1994A mouse Y chromosome gene encoded by a region essential for spermatogenesis and expression of male-specific minor histocompatibility antigens.Hum Mol Genet3873878
- 85. Kuroki Y, Toyoda A, Noguchi H, Taylor TD, Itoh T, et al. (2006) Comparative analysis of chimpanzee and human Y chromosomes unveils complex evolutionary pathway. Nat Genet 38: 158–167.Y. KurokiA. ToyodaH. NoguchiTD TaylorT. Itoh2006Comparative analysis of chimpanzee and human Y chromosomes unveils complex evolutionary pathway.Nat Genet38158167
- 86. Salido EC, Li XM, Yen PH, Martin N, Mohandas TK, et al. (1996) Cloning and expression of the mouse pseudoautosomal steroid sulphatase gene (Sts). Nat Genet 13: 83–86.EC SalidoXM LiPH YenN. MartinTK Mohandas1996Cloning and expression of the mouse pseudoautosomal steroid sulphatase gene (Sts).Nat Genet138386
- 87. Moore SS, Byrne K, Johnson SE, Kata S, Womack JE (2001) Physical mapping of CSF2RA, ANT3 and STS on the pseudoautosomal region of bovine chromosome X. Anim Genet 32: 102–104.SS MooreK. ByrneSE JohnsonS. KataJE Womack2001Physical mapping of CSF2RA, ANT3 and STS on the pseudoautosomal region of bovine chromosome X.Anim Genet32102104
- 88. Van Laere AS, Coppieters W, Georges M (2008) Characterization of the bovine pseudoautosomal boundary: Documenting the evolutionary history of mammalian sex chromosomes. Genome Res 18: 1884–1895.AS Van LaereW. CoppietersM. Georges2008Characterization of the bovine pseudoautosomal boundary: Documenting the evolutionary history of mammalian sex chromosomes.Genome Res1818841895
- 89. Mazeyrat S, Mitchell MJ (1998) Rodent Y chromosome TSPY gene is functional in rat and non-functional in mouse. Hum Mol Genet 7: 557–562.S. MazeyratMJ Mitchell1998Rodent Y chromosome TSPY gene is functional in rat and non-functional in mouse.Hum Mol Genet7557562