Treponema paraluiscuniculi is the causative agent of rabbit venereal spirochetosis. It is not infectious to humans, although its genome structure is very closely related to other pathogenic Treponema species including Treponema pallidum subspecies pallidum, the etiological agent of syphilis. In this study, the genome sequence of Treponema paraluiscuniculi, strain Cuniculi A, was determined by a combination of several high-throughput sequencing strategies. Whereas the overall size (1,133,390 bp), arrangement, and gene content of the Cuniculi A genome closely resembled those of the T. pallidum genome, the T. paraluiscuniculi genome contained a markedly higher number of pseudogenes and gene fragments (51). In addition to pseudogenes, 33 divergent genes were also found in the T. paraluiscuniculi genome. A set of 32 (out of 84) affected genes encoded proteins of known or predicted function in the Nichols genome. These proteins included virulence factors, gene regulators and components of DNA repair and recombination. The majority (52 or 61.9%) of the Cuniculi A pseudogenes and divergent genes were of unknown function. Our results indicate that T. paraluiscuniculi has evolved from a T. pallidum-like ancestor and adapted to a specialized host-associated niche (rabbits) during loss of infectivity to humans. The genes that are inactivated or altered in T. paraluiscuniculi are candidates for virulence factors important in the infectivity and pathogenesis of T. pallidum subspecies.
Citation: Šmajs D, Zobaníková M, Strouhal M, Čejková D, Dugan-Rocha S, Pospíšilová P, et al. (2011) Complete Genome Sequence of Treponema paraluiscuniculi, Strain Cuniculi A: The Loss of Infectivity to Humans Is Associated with Genome Decay. PLoS ONE 6(5): e20415. https://doi.org/10.1371/journal.pone.0020415
Editor: Cynthia Gibas, University of North Carolina at Charlotte, United States of America
Received: December 6, 2010; Accepted: May 2, 2011; Published: May 31, 2011
Copyright: © 2011 Šmajs et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants from the U.S. Public Health Service to G.M.W. (R01 DE12488 and R01 DE13759), and S.J.N. (R01 AI49252 and R03 AI69107) and by the grants of the Grant Agency of the Czech Republic (310/07/0321), and the Ministry of Education of the Czech Republic (VZ SM0021622415) to D.S. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NimbleGen Systems, Inc., provided oligonucleotide arrays and initial analysis of microarray data.
Competing interests: The authors have read the journal's policy and have the following conflicts: Tom Albert was employed by the NimbleGen Systems, Inc., and performed microarray hybridization and data analysis with no impact on the interpretation of data. The authors adhered to all the PLoS ONE policies on sharing data and materials.
Treponema paraluiscuniculi is a noncultivable species of the genus Treponema that causes venereal spirochetosis in rabbits, but that is not infectious to humans . The genome structure and chromosome sequence of T. paraluiscuniculi is closely related to other pathogenic species and subspecies of the Treponema genus, including syphilis causing spirochete Treponema pallidum ssp. pallidum  and Treponema pallidum ssp. pertenue, the causative agent of yaws.
The presence of spirochetes resembling T. pallidum in rabbit genital lesions was reported as early as 1912, and organism was described as Spirochaeta paralues-cuniculi (syphilis-like spirochetes in rabbits) by Jacobsthal . The naturally occurring infection of rabbits with T. paraluiscuniculi is described in a detailed historical review by Smith and Pesetsky , as well as in more recent articles by DiGiacomo et al. , . The disease is typically sexually transmitted, and results in erythema and edema of the prepuce, vagina, anus, or scrotum, often followed by ulceration and crusting (eschar formation) of the lesion. Infection of the nose, eyelids, lips, and paws can also occur. Intradermal inoculation of rabbits with either T. paraluiscuniculi or T. pallidum results in erythematous lesions that may undergo ulceration –. T. paraluiscuniculi lesions are noted to be less indurated (raised and hardened) than T. pallidum lesions. T. pallidum subspecies and T. paraluiscuniculi are nearly indistinguishable in terms of morphology, antigen content, and physiology –, consistent with the close genetic relationship among these organisms . However, T. paraluiscuniculi and T. pallidum cause different diseases with different host specificity. Rabbit venereal spirochetosis is characterized by localized genital lesions, whereas human syphilis is a multistage, sexually transmitted disease with varied clinical manifestations. Syphilis treponemes can infect virtually any human tissue, causing gummatous, neurologic, and cardiovascular manifestations , . While there is little evidence for systemic manifestations following T. paraluiscuniculi infection in rabbits, the organism can disseminate and be recovered from lymph nodes months after infection , .
Evidence to date indicates that T. paraluiscuniculi is not pathogenic to humans, indicating a basic difference between this organism and the T. pallidum subspecies. Two studies involving a total of three volunteers described experimental inoculation of humans ,  with rabbit virulent strains of T. paraluiscuniculi with negative results. When injected intradermally into human volunteers, rabbit virulent strains of T. paraluiscuniculi caused only mild, local erythema and edema (without systemic effects) that disappeared after three weeks , . In the Graves and Downes study , only a limited serological response in the human volunteer was observed. In contrast, intradermally inoculated rabbits exhibited prominent, long-lasting lesions and a strong serological response to T. paraluiscuniculi.
Partial immunological cross-protection between T. paraluiscuniculi and T. pallidum has been observed, as demonstrated by infecting rabbits with one species, inoculating intradermally with the other species 3–6 months later, and observing lesion development for signs of decreased lesion frequency, severity, or duration . Both serologic and T-cell reactivity indicate antigenic relatedness between these species , , , . Heterogenity in the paralogous tpr gene families of these organisms have been characterized , , and may be responsible in part for the pathogenic differences and incomplete immunologic cross-protection observed.
Differences in host specificity and in clinical manifestations of these diseases reflect the primary genetic differences between T. paraluiscuniculi and T. pallidum. In this communication, we report a complete genome sequence of T. paraluiscuniculi, strain Cuniculi A and compare this sequence to the published genomes of T. pallidum ssp. pallidum Nichols, SS14 and Chicago –.
T. paraluiscuniculi Cuniculi A genomic parameters and annotation
The summarized genomic features of T. paraluiscuniculi strain Cuniculi A are shown in the Table 1. The genome size of T. paraluiscuniculi Cuniculi A (1,133,390 bp) is 4.6, 5.9 and 6.1 kb smaller than the genome size of the previously published T. pallidum ssp. pallidum Nichols (1,138,011 bp) , Chicago (1,139,281 bp)  and SS14 genomes (1,139,457 bp) , respectively. Similar whole genome nucleotide diversity (π ± SD) of 0.01028±0.00514, 0.01021±0.00511, and 0.01016±0.00508, was revealed by DnaSP v5 software between Cuniculi A genome and Nichols, Chicago, and SS14 genomes, respectively. The deletions in the Cuniculi A genome were not evenly distributed in the genome and were predominantly localized in tpr loci and the vicinity of these regions , . The overall gene order in the Cuniculi A was identical to both Nichols and SS14 genomes. Out of 1,133,390 bp of the Cuniculi genome, 1,092,714 bp were aligned with the Nichols genome and this part of the genome contained 8074 single nucleotide replacements and additional 1124 bp changes in 224 individual indels. This corresponds to 99.16% sequence identity between the conserved regions of the Nichols and Cuniculi A genomes. Out of 1016 annotated genes encoding proteins in the Cuniculi A genome, gene function was predicted for 650 genes (64%). In addition to genes present in the Nichols genome, 57 hypothetical genes were newly annotated in the Cuniculi A genome. These genes were annotated in the orthologous sequences and did not represent a new genetic material specific to Cuniculi A. Instead, they represented differences in the recent annotation algorithms compared to the previously used ones . The average gene length of these 57 genes was of 234 nt (median length of 162 nt) indicating markedly shorter gene length compared to average and median gene length of all Cuniculi A genes (1006 and 873 bp, respectively). Seventeen automated gene predictions were omitted in the final annotation mainly due to overlap with other already annotated genes. 75 and 280 genes were annotated shorter or longer, respectively, in the Cuniculi A annotation when compared to the length of orthologous genes in the Nichols and SS14 genome annotations. 47 genes originally annotated in the Nichols genome  were not annotated in the Cuniculi A genome as a result of differences in gene prediction criteria. For all of these 47 genes, orthologous sequences were found in the Cuniculi A genome. All of these 47 genes encoded short hypothetical proteins with average gene length of 196.4 bp (median of 150 bp).
Alignment of the Cuniculi A genome with the annotated Nichols genes identified 84 orthologous Cuniculi A regions/genes containing internal frameshifts and/or major sequence changes. In the Cuniculi A genome, 63 genes (6.2% of 1016 protein-encoding genes) were annotated in these orthologous loci. Genes with major sequence changes were defined as those causing more than 10 continuous amino acid replacements (or indels) in the corresponding protein sequence or showing more than 20 dispersed amino acid changes in the Cuniculi A protein compared to the Nichols ortholog. Changes in protein length at the N-terminus resulting from predictions of longer Cuniculi A genes were not considered as major sequence changes if there was an existing potential downstream start codon at corresponding gene position as in the Nichols genome. A set of 134 (13.2% of 1016 protein-encoding genes) predicted Cuniculi A genes encoded identical proteins to predicted Nichols proteins and 819 genes (80.6%) encode proteins with one or several amino acid replacements.
Compared to the published Nichols genome, 52 Nichols orthologs were fused into 25 genes in the Cuniculi A genome (see Table S1). In two cases, three genes annotated in the Nichols genome were fused to one gene in the Cuniculi A genome (TP0006, TP0007, TP0008 and TP0174, TP0175, TP0176). Similar situation was also found in the genome of T. pallidum subspecies pertenue Samoa D where sequencing revealed fusion of 48 Nichols orthologs into 23 genes (data not shown). An ongoing resequencing of the Nichols genome (P. Pospíšilová, personal communication) revealed that most of the observed gene fusions are also present in the Nichols genome indicating that the published Nichols sequence  contains dozens of sequencing errors. To test whether these Nichols changes are sequencing errors or intrastrain adaptive mutations, we analyzed 208 nucleotide positions in which the resequenced Nichols genome differs from the published Nichols version  in 3 T. pallidum strains including Chicago  and preliminary DAL-1 and Mexico A whole genome sequences (unpublished data). Out of 208 nucleotide differences found in the resequenced Nichols genome, 179 (86.1%) were present in all 3 tested genomes, 12 (5.8%) were present in one or two other genomes and 17 (8.2%) were specific for the newly sequenced Nichols strain. The presence of majority of nucleotide changes (identified in the resequenced Nichols genome) in three other T. pallidum genomes indicates sequencing errors in the published Nichols genome  rather than recently emerged intrastrain adaptive mutations.
Whole genome fingerprinting and sequencing error rate
Whole genome fingerprinting was used to assess the overall genome assembly of the Cuniculi A genome. Fingerprinting of the Cuniculi A genome described earlier  was extended by additional restriction enzyme analyses to reduce the length of the resulting DNA fragments. The in silico restriction mapping was compared to experimentally obtained restriction digest patterns. In the final assembly, 2017 restriction target sites were experimentally verified resulting in average length of assessed DNA fragment of 562 bp. The 2017 restriction target sites corresponded to a total sequence length of 11,702 bp (1.0% of the genome length). Since no discrepancies between in silico and experimental restriction analysis were observed, the expected corresponding sequencing error rate in the Cuniculi A genome was set to the order of 10−4 or better.
T. paraluiscuniculi genes encoding identical proteins as corresponding orthologs in the Nichols genome
Altogether, 134 (13.2%) of Cuniculi A genes were found to encode identical proteins to those encoded in the Nichols genome (Table S2 and S3). 35 of these genes (Table S3) encoded proteins of unknown function and the remaining 99 genes encoded proteins involved in the translation of mRNA (32 genes), general metabolism (24 genes), transport (12 genes), flagellar synthesis (11 genes), gene regulation (6 genes) and other functions (14 genes). Conservation of these proteins and their predominant involvement in translation and general metabolism indicate that these genes are housekeeping genes under strong negative selection in the genus Treponema. The identical genes encoding proteins of unknown function (35, or 26.1%) may encode proteins needed for as yet unidentified essential functions, including general metabolic processes. Although the median RNA transcript level of these 35 genes in T. pallidum ssp. pallidum Nichols  is lower than that of the 99 identical genes with annotated functions (0.92 and 1.37, respectively), it is close to the median value for all genes with annotated functions (0.95).
Genes containing frameshifts and/or major sequence changes (MSC) in T. paraluiscuniculi functional gene groups
T. paraluiscuniculi genes were classified into 7 functional groups according to the modified classification used by Fraser et al.  (Table 2). A set of 161 genes (9.8% of all protein-encoding genes) encoding proteins involved in general metabolic functions was used as an internal standard for comparisons with genes from other functional groups. The number of genes containing frameshifts and/or major sequence changes (related to Nichols orthologs) was compared to the number of these genes present in the general metabolism group (1.9%, Table 2) and statistically significant differences were found in the group of virulence factors (41.9%, p<0.001), in genes with an unknown function (14.0%, p<0.001), and in genes involved in DNA metabolism (7.8%, p = 0.037).
Hypothetical genes with internal frameshifts and/or major sequence changes in the T. paraluiscuniculi genome
Altogether, 52 Cuniculi A hypothetical genes corresponding to 54 Nichols orthologs (Table S4) in the T. paraluiscuniculi genome showed frameshifts (21 genes), partial or complete gene deletions (7 genes), internal stop codons (2 genes) or multiple nucleotide changes (22 genes). Twelve of these proteins were predicted inner or outer membrane proteins and 9 of them were identified as antigens . Three of these 52 proteins (TP0133, TP0462, and TP0895) were predicted lipoproteins , and these 3 proteins were also identified as antigens. These results indicate that the T. paraluiscuniculi genome may be undergoing gene degradation and loss relative to T. pallidum subsp. pallidum, consistent with a decreased requirement for these genes in the rabbit tissue niche inhabited by T. paraluiscuniculi. Altogether, for 25 out these 52 Cuniculi A hypothetical genes, the Ka/Ks ratios and predicted type of selection were calculated (Table S4). Most of the genes (20) were found under neutral selection, while 4 genes under purifying and one under positive selection.
Cuniculi A genes with predicted cell function containing internal frameshifts and/or major sequence changes
Altogether, 32 Cuniculi A genes with defined or predicted functions were found to contain frameshifts or major deletions (resulting in 21 pseudogenes), major sequence changes (8 genes), or reverted frameshift mutations (3 genes) (Table 3). Ten out of these 32 genes were tpr genes encoding paralogous proteins with sequence similarity to the major surface protein (Msp) of Treponema denticola . A schematic representation of all tpr genes in the Cuniculi A genome is shown in Fig. 1. In addition to these potential virulence factors, another three proteins encoded by TPCCA_0136 (fibronectin-binding protein), TPCCA_0326 (tp92, outer membrane protein), and TPCCA_0433 (acidic repeat protein, Arp protein) showed indels and major sequence changes. Together with TPCCA_0760 (penicillin-binding protein), these proteins are important treponemal antigens and/or cell envelope structures –. Another gene potentially involved in cell wall biosynthesis, the Cuniculi A polysaccharide biosynthesis capD gene (TPCCA_0077), contained an internal stop codon predicted to result in gene inactivation. The Cuniculi A transmembrane chemoreceptors (Mcp proteins) either showed major sequence changes (TPCCA_0040 and TPCCA_0488, Table S4) or showed a relatively high number of amino acid replacements (TPCCA_0639, TPCCA_0640; 14 and 10 aa changes, respectively; data not shown).
Identities at nucleotide levels of Cuniculi A and Nichols genomes are shown. Colors indicate sequence similarities among paralogous tpr genes, i.e. sequence similarities within the T. paraluiscuniculi genome (e.g. tprC and tprD genes are identical). In the Cuniculi A genome, reverted frameshift mutation (in tprA), frameshift mutations (in tprC,D,E,F,G,J,K), deletions (in tprF,G,I) and gene elongation are present (in tprL). The tprF deletion shown in the Nichols genome is based on the tprF sequence taken from T. pallidum ssp. pertenue Samoa D genome (data not shown). In the Cuniculi A and Nichols tprK genes, shorter gene versions (starting with the next available downstream start codon) are expected rather than the presence of frameshift mutation in the Cuniculi A tprK.
Several Cuniculi A genes with frameshifts belonged to the family of genes with predicted regulatory functions including TPCCA_0220 (anti-sigma factor antagonist), TPCCA_0461 (probable transcriptional regulator), TPCCA_0511 (CarD family transcriptional regulator), and TPCCA_0520 (sensor histidine kinase). In contrast to TP0520 encoded in the genome of T. pallidum ssp. pallidum (strains Nichols and SS14) and in the T. pallidum ssp. pertenue (strains Samoa D, CDC-2, Gauthier; M. Zobaníková, D. Čejková, personal communication), TPCCA_0520 of T. paraluiscuniculi encodes full length sensor histidine kinase suggesting possible role of this protein in gene regulation. In addition to the previously mentioned tprA and TPCCA_0520 genes, reversion of frameshift mutation in TPCCA_0812 (fadD, probable long-chain-fatty-acid–CoA ligase) was also found in the Cuniculi A genome.
Four genes encoding proteins involved in DNA processing (RecB, RecQ, RecX, and Ssb) also had altered open reading frames in the Cuniculi A strain relative to the Nichols strain. The frameshift mutation in the recQ gene resulted in premature truncation of RecQ, whereas RecX and Ssb proteins were elongated at C-termini. RecB showed multiple amino acid changes. Other genes with major sequence changes involved gltD (TPCCA_0735, glutamate and proline biosynthesis), ushA (TPCCA_0104, bifunctional 5′-nucleotidase/UDP-sugar diphosphatase), TPCCA_0309 (probable polar amino acid ABC transporter), and mglB (TPCCA_0545, sugar ABC transporter).
For 11 out of these 32 Cuniculi A genes, selection type was predicted (Table 3). While majority of genes were under neutral (6) or purifying selection (3), two genes including tprL and arp were found under positive selection.
Taken together, genes encoding several potential virulence determinants, including Tpr proteins, other membrane proteins, cell wall biosynthesis proteins, gene regulatory proteins and components of the DNA repair process, are significantly altered in the T. paraluiscuniculi Cuniculi A genome relative to the T. pallidum genomes sequenced to date.
The complete genome sequence of T. paraluiscuniculi Cuniculi A was determined by combining the data obtained by Illumina and Sanger sequencing and microarray hybridization approaches. The 454 sequencing data were used as a scaffold for the assembly and the final genome sequence was verified by genomic fingerprinting. This analysis of the Cuniculi A genome revealed striking similarity with other sequenced treponemal genomes (99.16% sequence identity between the conserved regions of the Nichols and Cuniculi A genomes), including identical gene orders, despite the differences in the host specificity and clinical manifestations of infections caused by T. paraluiscuniculi and T. pallidum ssp. pallidum. The Cuniculi A genome size (1,133,390 bp) is about 4.6 and 6.1 kb smaller than the genome size of the previously published T. p. ssp. pallidum Nichols and SS14 genomes, respectively. Additionally, an insertion harboring a tprK-like sequence (1.3 kb) in the intergenic region between TP0126 and TP0127 is present in a subpopulation of the Nichols strain  resulting in 5.9 kb difference between Cuniculi A and Nichols genomes. In unpublished studies, the genome of T. pallidum subspecies pertenue Samoa D (1,139,330 bp) was also found to be ∼6.0 kb larger than the Cuniculi A genome (D. Čejková, personal communication). As shown by Strouhal et al. , the smaller Cuniculi A genome is a result of deletions localized mainly around tpr genes. In addition to decreased genome size, the Cuniculi A genome contained markedly higher number of pseudogenes and gene fragments. In the Nichols genome, only 9 genes containing authentic frameshifts were identified . Besides these 9 genes with authentic frameshifts there are additional 6 pseudogenes in the SS14 genome (5 genes with frameshifts and one with nonsense mutation) . In the genome of Samoa D, there are 13 pseudogenes (M. Zobaníková, personal communication). The number of pseudogenes and gene fragments (51) in the Cuniculi A genome thus markedly exceeds the number in Nichols, SS14 and Samoa D genomes, respectively.
In the Cuniculi A genome, there are 25 genes representing fusions of 52 Nichols orthologs. In most cases, Nichols orthologs are separated because of sequencing errors in the published whole genome Nichols sequence . Therefore, most of these gene fusions do not represent true differences in the compared genomes. In contrast, missing genes, gene fragments, pseudogenes, and genes encoding proteins with many amino acid changes were considered important differences.
About 13% of the Cuniculi A genes were found to encode identical proteins to those encoded in the Nichols genome, indicating strong conservation of protein sequence in these proteins. Most of these genes encoded housekeeping proteins and likely represent a set of highly conserved treponemal genes important for basic treponemal cellular functions. The 35 genes encoding identical hypothetical proteins in both genomes are also candidates for important cellular functions. Indeed, the functions of three of these proteins were predicted recently: TP0650 as a protein involved in translation, TP0772 as a transcriptional regulator and TP0941 as a regulator of motility .
Comparison of number of genes containing frameshifts and/or major sequence changes (when compared to Nichols orthologs) within the Cuniculi A functional gene groups revealed a high percentage of these genes in the group of virulence factors, in genes involved in DNA metabolism, and in a group of genes with unknown function. These findings suggest that the accumulation of changes in genes encoding predicted virulence factors and genes with unknown functions (some of them are potential candidates for virulence factors, see below) is the reason for the loss of T. paraluiscuniculi infectivity to humans. Moreover, affected genes involved in DNA replication, repair, and recombination could suggest their possible role in the acceleration of T. paraluiscuniculi evolution.
Fifty-two hypothetical genes with internal frameshifts and/or major sequence changes were found in the T. paraluiscuniculi genome (Table 2 and S4). The data available from the previously published transcriptome analysis that mapped the Nichols strain gene expression levels during experimental rabbit infection  clearly showed that these genes are actively transcribed during infection. Moreover, the median transcript levels of these genes was considerably higher than the median gene expression rate of all genes of unknown function (1.46 versus 0.86) indicating that these genes are likely to represent true genes in the Nichols genome playing an important role during infection. These hypothetical genes are therefore candidates for important virulence factors of T. pallidum and should attract interest in future syphilis research. In T. paraluiscuniculi, most of the genes (where the type of selection could be calculated) belonging to this group were found under neutral selection suggesting a genetic inactivity of these genes. The one positively selected gene, TP0031, could represent gene involved in adaptation of T. paraluiscuniculi to rabbits. In addition, 12 of hypothetical genes with internal frameshifts and/or major sequence changes were predicted to be inner or outer membrane proteins. T. pallidum whole–genome antigen screen, which tested 882 gene products , identified 106 antigens recognized by rabbit antibodies obtained from infected rabbits. Out of these 106 identified antigens, 9 antigens correspond to the group of 52 Cuniculi A hypothetical protein genes that have frameshifts or major sequence changes. Interestingly, the group of predicted membrane and outer membrane proteins and the group of identified antigens did not overlap, probably as a result of problematic recombinant production of membrane proteins in E. coli. In addition, 22 hypothetical proteins were identified to interact with proteins of known function including predominantly cell wall structures and antigens, regulatory and metabolic proteins . Altogether, more than half of these proteins are likely involved in cell wall structure, and several others might be involved in gene regulation.
The most affected group of genes in the Cuniculi A genome was the family of paralogous tpr genes. Although the precise role of individual tpr genes in the treponemal infection remains unclear, there is an expanding evidence of the role of tpr genes in treponemal pathogenicity and host specificity. In the genome of T. pallidum subsp. pallidum Nichols, there are 12 paralogous tpr genes . It was shown that Tpr proteins induced an antibody response during infection, and exhibit heterogeneity both within and between the T. pallidum subspecies and strains examined –. Tpr proteins are thought to be involved in pathogenesis and/or immune evasion. A model of gene conversion-driven antigenic variation of TprK during experimental infection was proposed . Differences between Treponema species and subspecies in tpr gene content and expression are thought to be important determinants of pathogenesis and immunogenicity , . Out of 12 tpr genes in the Cuniculi A genome (Fig. 1), only four are intact: tprA, tprB, tprH and tprL. The remaining 8 genes contain frameshifts and/or deletions. Interestingly, the tprA gene in the Nichols genome contains an authentic frameshift resulting in inactive tprA gene. This also applies for the SS14 genome . In contrast, tprA in the Cuniculi A (and also in the Samoa D genome; D. Čejková, personal communication) did not contain this frameshift mutation and appears to be functional. In addition to a regular copy of tprK, T. pallidum contains a tprK-like sequence localized in the 1.3 kb insertion present in a part of treponemal population . A similar situation also applies for T. p. ssp. pertenue genomes, where this site also showed intrastrain heterogeneity (data not shown). The region containing the tprK-like sequence was not found in the T. paraluiscuniculi . The major differences observed in tpr genes between T. paraluiscuniculi and T. pallidum suggest their role in the host range specificity. In fact, only tprA,B,H,L genes appear to be functional in the Cuniculi A genome. In contrast, tprC,D,E,F,G,I,J,K were affected in the Cuniculi A genome. The tprE,G,J genes were shown to be variably expressed in individual T. pallidum clinical isolates with guanosine homopolymers in promoter regions modulating their gene expression . Interestingly, six tpr genes including tprC,D,E,F,I,J out of 8 affected were recently predicted as genes encoding rare outer membrane proteins (OMPs) in T. pallidum . These findings suggest the role of Tpr rare OMPs in T. pallidum infectivity to humans. Out of 4 functional tprA,B,H,L genes, the tprB,L were predicted to encode rare OMPs (T. paraluiscuniculi TprL had a predicted signal sequence), suggesting that OMPs may be important also during T. paraluiscuniculi infection of rabbits.
There appear to be important differences between T. paraluiscuniculi Cuniculi A and other treponemal strains with regard to DNA recombination genes. In the Cuniculi A strain, the mutation in recQ resulted in a predicted RecQ protein without C-terminal, HDCR domain . Although the precise role of this domain remains unclear, the C-terminal RecQ domain binds DNA . The predicted sequence of RecB (important in the RecBCD pathway) has a high number of amino acid changes, and the predicted RecX and Ssp proteins have extended C-terminal sequences. There are also differences in the location of recX in Cuniculi A relative to other bacterial species and treponemal strains. In E. coli, the RecX protein was shown to inhibit some RecA-mediated functions . In contrast, in an exclusive human pathogen, N. gonorrhoeae, RecX enhances RecA activity . Similarly to situation in N. gonorrhoeae genome, recX in Cuniculi A is not located downstream of (and overlapping with) recA but is in a different genome locus; this difference indicates that RecX expression and function may be more similar to those in N. gonorrhoeae. Taken together, genetic changes in the recB, recQ and recX genes are consistent with observed increased genetic diversity in the Cuniculi A genome due to an ineffective/modified DNA repair and homologous recombination pathways. Homologous recombination as a major DNA repair process occurs frequently in the bacterial genomes and results in gene conversion. Gene conversion is an important mechanism of evolution of paralogous genes , and may be affected in the Cuniculi A genome.
We also examined other genetic differences to assess their potential role in pathogenesis patterns in T. paraluiscuniculi. In contrast to TPASS_0520 in T. p. ssp. pallidum and several other treponemes, TPCCA_0520 of T. paraluiscuniculi encodes full length sensor histidine kinase suggesting possible role of this protein in gene regulation. tprA and TPCCA_0520 genes are two out of four examples of intact genes present in the Cuniculi A genome but containing frameshift mutations in the ssp. pallidum. Several Cuniculi A membrane chemoreceptor proteins (Mcp's) contained major sequence changes (TPCCA_0040, TPCCA_0488, TPCCA_0639, TPCCA_0640) when compared to their Nichols counterparts. Chemotactic proteins thus seem to be one of the most divergent proteins in the Cuniculi A genome, which may correlate with altered chemotaxis signaling patterns.
Several bacterial envelope components contained major sequence changes. Arp protein is of unknown function but it contains repeated predicted fibronectin-binding immunogenic domains . Moreover, diverse repeats were shown to be associated with sexual transmission route of treponemal pathogens . Interestingly, the arp gene was found under a strong positive selection in the Cuniculi A genome, supporting its potential role in rabbit infection. TPCCA_0136, fibronectin- and laminin-binding protein is an outer membrane protein showing both inter- and intrasubspecies variable sequences. Immunization with recombinant protein delayed ulceration but did not prevent infection or the formation of lesions . The immunization with recombinant Tp92 partially protected rabbits from subsequent T. pallidum challenge . Sequence changes in genes encoding important antigens are one of the most probable reasons for changes in pathogenicity and host specificity. In Rickettsia prowazekii, the capD gene codes for an epimerase involved in capsular polysaccharide biosynthesis. The Cuniculi A capD was inactive. Since exopolysaccharides are important bacterial virulence factors, capD mutation in the Cuniculi A may be one of the reasons for decreased virulence of this strain. Beside these genes, altered genes with predicted regulatory functions (TPCCA_0220, TPCCA_0461, and TPCCA_0511) suggested differences in the gene regulatory network in the Cuniculi A genome. Changed regulatory network in the Cuniculi A genome and the resulting down- or upregulation of individual genes could be added to potential reasons for observed decreased virulence of Cuniculi A strain.
Taken together, the decreased size of the genome, marked increase in number of pseudogenes, affected genes involved in cell envelope biosynthesis and structure and multiple genetic changes in the proteins involved in DNA recombination, cell signaling and gene regulation appear to be the major reasons for narrower host specificity. Downsizing of the genome and accumulation of pseudogenes is common for bacteria adapting to simpler host-associated niches . The loss of infectivity of T. paraluiscuniculi to humans may represent such a process. On the other hand, adaptation of T. paraluiscuniculi to rabbits resulting in more efficient infection of this host could be a result of additional changes that may include positively selected tprL, arp, and TP0031 genes and/or a number of dispersed mutations throughout the T. paraluiscuniculi genome. T. paraluiscuniculi thus appears to be treponeme in the process of adaptation to a single host (rabbit) and therefore is likely to be a descendant of pallidum- or pertenue-like ancestors rather than the opposite.
Materials and Methods
Isolation of T. paraluiscuniculi chromosomal DNA
T. paraluiscuniculi strain Cuniculi A was initially isolated from an infected rabbit by Drs. Paul Hardy and Ellen Nell; the strain was kindly provided by Dr. Sheila A. Lukehart at the University of Washington. Organisms were propagated by intratesticular inoculation of rabbits, extracted, and purified by Hypaque gradient centrifugation as described previously , . Genomic DNA was prepared according to the protocol published earlier .
The Cuniculi A genomic DNA (2.1 µg) was used for sequencing-by-synthesis (based on pyrosequencing) using GS20 sequencing machine (454 Life Sciences Corporation, Branford, CT, USA). Sequencing resulted in 398 individual trimmed contigs with a total contig size of 1,133,704 bp (average contig length of 2848 bp, contig length ranging from 102 to 22,217 bp). A subset of 330 individual contigs showed hits to published Nichols treponemal DNA , covering 1,128,602 bp and leaving 4,788 bp unsequenced. The number of individual reads in these 330 contigs was 204,765 representing total read length of 20,443,023 bp. The corresponding sequencing coverage for the Cuniculi A genome was 18.04. Most of contings not related to treponemes showed similarity to rabbit sequences, probably as a result of contamination of Cuniculi A DNA by rabbit DNA during preparation of chromosomal DNA.
Parallel to pyrosequencing, an Illumina (Illumina, San Diego, CA, USA) sequencing approach was employed using the Genome Analyzer sequencing machine. Illumina reads (36 bp each) were assembled into 726 contigs using Velvet short read assembler . Total number of reads (3,053,564) represented total read length of 109,928,304 bp (97x coverage). Some of the 726 contigs overlap by few bp and therefore the number of gaps dropped to the number 475, representing total gap length of 33,634 bp.
Sanger sequencing of Cuniculi A sequences was used to assess the quality of DNA sequencing and to finish the whole genome sequence. Approximately 150 PCR products, generated with primers used for other treponemal genome projects were sequenced to provide a comparison to the Illumina- and 454-generated sequences. All 50 discrepancies were in the 454 sequence results, and included 43 false insertions, 3 false deletions and 4 substitutions. Twenty-four out of 46 indels were found in homopolymeric regions). Based on these results, we considered the Illumina sequences to be more accurate and utilized them for generation of the complete genome sequence.
An additional approach, the CGS strategy  was used for determination of the Cuniculi A genome sequence. Oligonucleotide arrays of 29-mers derived from the Nichols sequence, covering both strands, were hybridized separately to fluorescently-labeled Nichols and Cuniculi A genomic DNA. Equal hybridization signals in both preparations of genomic DNA indicated identical sequences, whereas decreased hybridization with the Cuniculi A DNA occurred in regions with sequence differences or indels. This information was used to help ‘fill in’ the sequence gaps between Illumina contigs. All discrepancies in gap regions were resolved by traditional Sanger sequencing.
Whole genome fingerprinting
Whole genome fingerprinting  results for the Cuniculi A genome  were used for verification of the genome assembly. Briefly, primers designed for T. pallidum subsp. pallidum and template DNA from T. paraluiscuniculi Cuniculi A were used to produce large, 5 to 28 kb amplicons spanning the entire genome; these were then digested with multiple enzymes to provide a macro restriction map. The Cuniculi A genomic sequence was used for simulated restriction digest in silico and these data were compared with experimentally obtained data. Altogether, 19 individual restriction enzymes were used including Acc I (194 verified restriction target sites), Asc I (2), BamH I (222), Cla I (107), EcoR I (157), EcoR V (200), Hind III (258), Kpn I (112), Mlu I (277), Mse I (8), Nco I (61), Nde I (1), Nhe I (14), Rsr II (20), Sac I (86), Spe I (25), Sph I (13), Xba I (68) or Xho I (191) enzymes (NEB) either alone or in combinations. Three enzymes, BamH I, EcoR I and Hind III, were used for restriction analysis of all amplicons. The use of other enzymes was optional depending on length of restriction fragments and the availability of restriction target sites. To ascertain the experimental error of WGF, the lengths of 250 individual DNA fragments in 5 fragment intervals (50 fragments per interval) including 0.2–0.5 kb, 0.5–1 kb, 1–2 kb, 2–3 kb, and 3–4 kb, respectively, were measured from agarose gels by AlphaView Software Version 3.0 (Alpha Innotech, San Leandro, CA) and calculated from in silico data. The average error for each interval represented an average difference between experimental and calculated fragments sizes. The average errors were calculated to 10.9 bp, 16.8 bp, 22.3 bp, 38.9 bp and 52.5 bp in 0.2–0.5 kb, 0.5–1 kb, 1–2 kb, 2–3 kb and 3–4 kb intervals, respectively. A set of the smallest available 722 fragments (covering the entire Cuniculi A genome), with length ranging between 0.2 and 4.0 kb (average length of 1712 bp) and covering slightly more than the length of Cuniculi A genome due to overlaps of amplified regions (1,235,806 bp), were selected and the error rate was calculated based on number of DNA fragments in each individual size interval. The average error for all analyzed DNA restriction fragments was calculated to 27.9 bp (1.6% of average fragment length) with the variation range between 0 and 132 bp.
Gene prediction and annotation.
Gene prediction and annotation was performed according to the automated annotation scheme used at The Genome Center at Washington University . Genes (TPCCA genes) were predicted by the Glimmer and GeneMark programs , . The automated annotation was modified by comparison with the published genome sequences of the T. pallidum Nichols and SS14 genomes , . Genome alignments were performed using the Consed finishing tool .
DNA sequence analyses and statistical analyses
DNA sequence analyses were performed using the DnaSP software, version 5.10 . Whole genome nucleotide diversity (π) between Cuniculi A and individual sequenced T. pallidum genomes including Nichols, Chicago and SS14 strains was calculated, respectively. The number of synonymous substitutions per a synonymous site (Ks), the number of nonsynonymous substitutions per a nonsynonymous site (Ka), the Ka/Ks ratios, and the codon-based test for estimation of selection type were calculated using the Kumar model  and the MEGA4 software . Statistical significance of the number of genes containing frameshifts and/or major sequence changes (MSC) in T. paraluiscuniculi functional gene groups was calculated using standard methods derived from the binomial distribution, including the two-tailed test. For statistical calculations, STATISTICA program, version 8.0, (StatSoft, Tulsa, OK, USA) was used.
Genes fused in the T. paraluiscuniculi Cuniculi A genome when compared to the previously annotated T. pallidum subsp. pallidum Nichols genome  .
99 genes encoding identical proteins in T. paraluiscuniculi Cuniculi A and T. pallidum subsp. pallidum Nichols genomes.
35 genes of unknown function encoding identical proteins in T. paraluiscuniculi Cuniculi A and T. pallidum subsp. pallidum Nichols genomes.
We like to acknowledge Donna Villasana, Zhangwan Li and Jing Liu for technical assistance in DNA sequencing.
Conceived and designed the experiments: DS GMW. Performed the experiments: DS MZ MS DC PP SD-R. Analyzed the data: TA DS MZ XQ CB LC KH-P DMM. Contributed reagents/materials/analysis tools: DS GMW RAG SJN TA. Wrote the paper: DS SJN.
- 1. Graves S, Downes J (1981) Experimental infection of man with rabbit-virulent Treponema paraluis-cuniculi. Br J Vener Dis 57: 7–10.S. GravesJ. Downes1981Experimental infection of man with rabbit-virulent Treponema paraluis-cuniculi.Br J Vener Dis57710
- 2. Strouhal M, Šmajs D, Matějková P, Sodergren E, Amin AG, et al. (2007) Genome differences between Treponema pallidum subsp pallidum strain Nichols and T. paraluiscuniculi strain Cuniculi A. Infect Immun 75: 5859–5866.M. StrouhalD. ŠmajsP. MatějkováE. SodergrenAG Amin2007Genome differences between Treponema pallidum subsp pallidum strain Nichols and T. paraluiscuniculi strain Cuniculi A.Infect Immun7558595866
- 3. Jacobsthal E (1920) Untersuchungen über eine syphilisähnliche Spontanerkrankung des Kaninchens (Paralues-cuniculi). Derm Wschr 71: 569–571.E. Jacobsthal1920Untersuchungen über eine syphilisähnliche Spontanerkrankung des Kaninchens (Paralues-cuniculi).Derm Wschr71569571
- 4. Smith JL, Pesetsky BR (1967) The current status of Treponema cuniculi: Review of the literature. Br J Vener Dis 43: 117–127.JL SmithBR Pesetsky1967The current status of Treponema cuniculi: Review of the literature.Br J Vener Dis43117127
- 5. DiGiacomo RF, Talburt CD, Lukehart SA, Baker-Zander SA, Condon J (1983) Treponema paraluis-cuniculi infection in a commercial rabbitry: epidemiology and serodiagnosis. Lab Anim Sci 33: 562–566.RF DiGiacomoCD TalburtSA LukehartSA Baker-ZanderJ. Condon1983Treponema paraluis-cuniculi infection in a commercial rabbitry: epidemiology and serodiagnosis.Lab Anim Sci33562566
- 6. DiGiacomo RF, Lukehart SA, Talburt CD, Baker-Zander SA, Condon J, et al. (1984) Clinical course and treatment of venereal spirochaetosis in New Zealand white rabbits. Br J Vener Dis 60: 214–218.RF DiGiacomoSA LukehartCD TalburtSA Baker-ZanderJ. Condon1984Clinical course and treatment of venereal spirochaetosis in New Zealand white rabbits.Br J Vener Dis60214218
- 7. Schell RF, Azadegan AA, Nitskansky SG, Lefrock JL (1982) Acquired resistance of hamsters to challenge with homologous and heterologous virulent treponemes. Infect Immun 37: 617–621.RF SchellAA AzadeganSG NitskanskyJL Lefrock1982Acquired resistance of hamsters to challenge with homologous and heterologous virulent treponemes.Infect Immun37617621
- 8. Turner TB, Hollander DH (1957) Biology of the treponematoses. Geneva: World Health Organization. 272 p.TB TurnerDH Hollander1957Biology of the treponematoses.GenevaWorld Health Organization272
- 9. Baker-Zander SA, Lukehart SA (1984) Antigenic cross-reactivity between Treponema pallidum and other pathogenic members of the family Spirochaetaceae. Infect Immun 46: 116–121.SA Baker-ZanderSA Lukehart1984Antigenic cross-reactivity between Treponema pallidum and other pathogenic members of the family Spirochaetaceae.Infect Immun46116121
- 10. Hougen KH, Birch-Andersen A, Jensen HJ (1973) Electron microscopy of Treponema cuniculi. Acta Pathol Microbiol Scand Microbiol Immunol 81: 15–28.KH HougenA. Birch-AndersenHJ Jensen1973Electron microscopy of Treponema cuniculi.Acta Pathol Microbiol Scand Microbiol Immunol811528
- 11. Norris SJ, Pope V, Johnson RE, Larsen SA (2003) Treponema and other human host-associated spirochetes. In: Murray PR, Baron EJ, Pfaller MA, Jorgensen JH, Yolken RH, editors. Manual of Clinical Microbiology. Washington DC: ASM Press. pp. 955–971.SJ NorrisV. PopeRE JohnsonSA Larsen2003Treponema and other human host-associated spirochetes.PR MurrayEJ BaronMA PfallerJH JorgensenRH YolkenManual of Clinical MicrobiologyWashington DCASM Press955971
- 12. Peeling RW, Hook EW (2006) The pathogenesis of syphilis: the Great Mimicker, revisited. J Pathol 208: 224–232.RW PeelingEW Hook2006The pathogenesis of syphilis: the Great Mimicker, revisited.J Pathol208224232
- 13. Digiacomo RF, Lukehart SA, Talburt CD, Baker-Zander SA, Giddens WE, et al. (1985) Chronicity of infection with Treponema paraluis-cuniculi in New Zealand white rabbits. Genitourin Med 61: 156–164.RF DigiacomoSA LukehartCD TalburtSA Baker-ZanderWE Giddens1985Chronicity of infection with Treponema paraluis-cuniculi in New Zealand white rabbits.Genitourin Med61156164
- 14. Levaditi C, Marie A, Nicolau S (1921) Virulence pour l'homme du spirochète de la spirillose spontanée du lapin. C R Acad Sci 172: 1542–1543.C. LevaditiA. MarieS. Nicolau1921Virulence pour l'homme du spirochète de la spirillose spontanée du lapin.C R Acad Sci17215421543
- 15. Khan AS, Nelson RAJ, Turner TB (1951) Immunological relationships among species and strains of virulent treponemes as determined with the treponemal immobilization test. Am J Hyg 53: 296–316.AS KhanRAJ NelsonTB Turner1951Immunological relationships among species and strains of virulent treponemes as determined with the treponemal immobilization test.Am J Hyg53296316
- 16. Giacani L, Sun ES, Hevner K, Molini BJ, Van Voorhis WC, et al. (2004) Tpr homologs in Treponema paraluiscuniculi Cuniculi A strain. Infect Immun 72: 6561–6576.L. GiacaniES SunK. HevnerBJ MoliniWC Van Voorhis2004Tpr homologs in Treponema paraluiscuniculi Cuniculi A strain.Infect Immun7265616576
- 17. Gray RR, Mulligan CJ, Molini BJ, Sun ES, Giacani L, et al. (2006) Molecular evolution of the tprC, D, I, K, G, and J genes in the pathogenic genus Treponema. Mol Biol and Evol 23: 2220–2233.RR GrayCJ MulliganBJ MoliniES SunL. Giacani2006Molecular evolution of the tprC, D, I, K, G, and J genes in the pathogenic genus Treponema.Mol Biol and Evol2322202233
- 18. Fraser CM, Norris SJ, Weinstock GM, White O, Sutton GG, et al. (1998) Complete genome sequence of Treponema pallidum, the syphilis spirochete. Science 281: 375–388.CM FraserSJ NorrisGM WeinstockO. WhiteGG Sutton1998Complete genome sequence of Treponema pallidum, the syphilis spirochete.Science281375388
- 19. Matějková P, Strouhal M, Šmajs D, Norris SJ, Palzkill T, et al. (2008) Complete genome sequence of Treponema pallidum ssp pallidum strain SS14 determined with oligonucleotide arrays. BMC Microbiol 8: 76.P. MatějkováM. StrouhalD. ŠmajsSJ NorrisT. Palzkill2008Complete genome sequence of Treponema pallidum ssp pallidum strain SS14 determined with oligonucleotide arrays.BMC Microbiol876
- 20. Giacani L, Jeffrey BM, Molini BJ, Le HT, Lukehart SA, et al (2010) Complete genome sequence and annotation of the Treponema pallidum subsp. pallidum Chicago strain. J Bacteriol 192: 2645–2646.L. GiacaniBM JeffreyBJ MoliniHT Leal Lukehart SA, et2010Complete genome sequence and annotation of the Treponema pallidum subsp. pallidum Chicago strain.J Bacteriol19226452646
- 21. Šmajs D, McKevitt M, Howell JK, Norris SJ, Cai WW, et al. (2005) Transcriptome of Treponema pallidum: Gene expression profile during experimental rabbit infection. J Bacteriol 187: 1866–1874.D. ŠmajsM. McKevittJK HowellSJ NorrisWW Cai2005Transcriptome of Treponema pallidum: Gene expression profile during experimental rabbit infection.J Bacteriol18718661874
- 22. McKevitt M, Brinkman MB, McLoughlin M, Perez C, Howell JK, et al. (2005) Genome scale identification of Treponema pallidum antigens. Infect Immun 73: 4445–4450.M. McKevittMB BrinkmanM. McLoughlinC. PerezJK Howell2005Genome scale identification of Treponema pallidum antigens.Infect Immun7344454450
- 23. Setubal JC, Reis M, Matsunaga J, Haake DA (2006) Lipoprotein computational prediction in spirochaetal genomes. Microbiology 152: 113–121.JC SetubalM. ReisJ. MatsunagaDA Haake2006Lipoprotein computational prediction in spirochaetal genomes.Microbiology152113121
- 24. Fenno J, Müller KH, McBride BC (1996) Sequence analysis, expression, and binding activity of recombinant major outer sheath protein (Msp) of Treponema denticola. J Bacteriol 178: 2489–2497.J. FennoKH MüllerBC McBride1996Sequence analysis, expression, and binding activity of recombinant major outer sheath protein (Msp) of Treponema denticola.J Bacteriol17824892497
- 25. Cameron CE, Lukehart SA, Castro C, Molini B, Godornes C, et al. (2000) Opsonic potential, protective capacity, and sequence conservation of the Treponema pallidum subspecies pallidum Tp92. J Infect Dis 181: 1401–1413.CE CameronSA LukehartC. CastroB. MoliniC. Godornes2000Opsonic potential, protective capacity, and sequence conservation of the Treponema pallidum subspecies pallidum Tp92.J Infect Dis18114011413
- 26. Brinkman MB, McGill MA, Pettersson J, Rogers A, Matejkova P, et al. (2008) A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin. Infect Immun 76: 1848–1857.MB BrinkmanMA McGillJ. PetterssonA. RogersP. Matejkova2008A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin.Infect Immun7618481857
- 27. Liu H, Rodes B, George R, Steiner B (2007) Molecular characterization and analysis of a gene encoding the acidic repeat protein (Arp) of Treponema pallidum. J Med Microbiol 56: 715–721.H. LiuB. RodesR. GeorgeB. Steiner2007Molecular characterization and analysis of a gene encoding the acidic repeat protein (Arp) of Treponema pallidum.J Med Microbiol56715721
- 28. Šmajs D, McKevitt M, Wang L, Howell JK, Norris SJ, et al. (2002) BAC library of T. pallidum DNA in E. coli. Genome Res 12: 515–522.D. ŠmajsM. McKevittL. WangJK HowellSJ Norris2002BAC library of T. pallidum DNA in E. coli.Genome Res12515522
- 29. Titz B, Rajagopala SV, Goll J, Häuser R, McKevitt MT, et al. (2008) The Binary protein interactome of Treponema pallidum - the syphilis spirochete. PLoS One 3: e2292.B. TitzSV RajagopalaJ. GollR. HäuserMT McKevitt2008The Binary protein interactome of Treponema pallidum - the syphilis spirochete.PLoS One3e2292
- 30. Centurion-Lara A, Castro C, Barrett L, Cameron C, Mostowfi M, et al. (1999) Treponema pallidum major sheath protein homologue Tpr K is a target of opsonic antibody and the protective immune response. J Exp Med 189: 647–656.A. Centurion-LaraC. CastroL. BarrettC. CameronM. Mostowfi1999Treponema pallidum major sheath protein homologue Tpr K is a target of opsonic antibody and the protective immune response.J Exp Med189647656
- 31. Centurion-Lara A, Godornes C, Castro C, Van Voorhis WC, Lukehart SA (2000a) The tprK gene is heterogeneous among Treponema pallidum strains and has multiple alleles. Infect Immun 68: 824–831.A. Centurion-LaraC. GodornesC. CastroWC Van VoorhisSA Lukehart2000aThe tprK gene is heterogeneous among Treponema pallidum strains and has multiple alleles.Infect Immun68824831
- 32. Centurion-Lara A, Sun ES, Barrett LK, Castro C, Lukehart SA, et al. (2000b) Multiple alleles of Treponema pallidum repeat gene D in Treponema pallidum isolates. J Bacteriol 182: 2332–2335.A. Centurion-LaraES SunLK BarrettC. CastroSA Lukehart2000bMultiple alleles of Treponema pallidum repeat gene D in Treponema pallidum isolates.J Bacteriol18223322335
- 33. Centurion-Lara A, LaFond RE, Hevner K, Godornes C, Molini BJ, et al. (2004) Gene conversion: a mechanism for generation of heterogeneity in the tprK gene of Treponema pallidum during infection. Mol Microbiol 52: 1579–1596.A. Centurion-LaraRE LaFondK. HevnerC. GodornesBJ Molini2004Gene conversion: a mechanism for generation of heterogeneity in the tprK gene of Treponema pallidum during infection.Mol Microbiol5215791596
- 34. Giacani L, Lukehart S, Centurion-Lara A (2007) Length of guanosine homopolymeric repeats modulates promoter activity of subfamily II tpr genes of Treponema pallidum ssp. pallidum. FEMS Immunol Med Microbiol 51: 289–301.L. GiacaniS. LukehartA. Centurion-Lara2007Length of guanosine homopolymeric repeats modulates promoter activity of subfamily II tpr genes of Treponema pallidum ssp. pallidum.FEMS Immunol Med Microbiol51289301
- 35. Cox DL, Luthra A, Dunham-Ems S, Desrosiers DC, Salazar JC, et al. (2010) Surface immunolabeling and consensus computational framework to identify candidate rare outer membrane proteins of Treponema pallidum. Infect Immun 78: 5178–5194.DL CoxA. LuthraS. Dunham-EmsDC DesrosiersJC Salazar2010Surface immunolabeling and consensus computational framework to identify candidate rare outer membrane proteins of Treponema pallidum.Infect Immun7851785194
- 36. Morozov V, Mushegian AR, Koonin EV, Bork P (1997) A putative nucleic acid-binding domain in Bloom's and Werner's syndrome helicases. Trends in Biochem Sci 22: 417–418.V. MorozovAR MushegianEV KooninP. Bork1997A putative nucleic acid-binding domain in Bloom's and Werner's syndrome helicases.Trends in Biochem Sci22417418
- 37. Bernstein DA, Keck JL (2003) Domain mapping of Escherichia coli RecQ defines the roles of conserved N- and C-terminal regions in the RecQ family. Nucleic Acids Res 31: 2778–2785.DA BernsteinJL Keck2003Domain mapping of Escherichia coli RecQ defines the roles of conserved N- and C-terminal regions in the RecQ family.Nucleic Acids Res3127782785
- 38. Stohl EA, Brockman JP, Burkle KL, Morimatsu K, Kowalczykowski SC, et al. (2003) Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo. J Biol Chem 278: 2278–2285.EA StohlJP BrockmanKL BurkleK. MorimatsuSC Kowalczykowski2003Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo.J Biol Chem27822782285
- 39. Stohl EA, Seifert HS (2001) The recX gene potentiates homologous recombination in Neisseria gonorrhoeae. Mol Microbiol 40: 1301–1310.EA StohlHS Seifert2001The recX gene potentiates homologous recombination in Neisseria gonorrhoeae.Mol Microbiol4013011310
- 40. Noonan JP, Grimwood J, Schmutz J, Dickson M, Myers RM (2004) Gene conversion and the evolution of protocadherin gene cluster diversity. Genome Res 14: 354–366.JP NoonanJ. GrimwoodJ. SchmutzM. DicksonRM Myers2004Gene conversion and the evolution of protocadherin gene cluster diversity.Genome Res14354366
- 41. Harper KN, Liu H, Ocampo PS, Steiner BM, Martin A, et al. (2008) The sequence of the acidic repeat protein (arp) gene differentiates venereal from nonvenereal Treponema pallidum subspecies, and the gene has evolved under strong positive selection in the subspecies that causes syphilis. FEMS Immunol Med Microbiol 53: 322–332.KN HarperH. LiuPS OcampoBM SteinerA. Martin2008The sequence of the acidic repeat protein (arp) gene differentiates venereal from nonvenereal Treponema pallidum subspecies, and the gene has evolved under strong positive selection in the subspecies that causes syphilis.FEMS Immunol Med Microbiol53322332
- 42. Pallen MJ, Wren BW (2007) Bacterial pathogenomics. Nature 449: 835–842.MJ PallenBW Wren2007Bacterial pathogenomics.Nature449835842
- 43. Baseman JB, Nichols JC, Rumpp JW, Hayes NS (1974) Purification of Treponema pallidum from infected rabbit tissue: resolution into two treponemal populations. Infect Immun 10: 1062–1067.JB BasemanJC NicholsJW RumppNS Hayes1974Purification of Treponema pallidum from infected rabbit tissue: resolution into two treponemal populations.Infect Immun1010621067
- 44. Zerbino DR, Birney E (2008) Velvet: Algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18: 821–829.DR ZerbinoE. Birney2008Velvet: Algorithms for de novo short read assembly using de Bruijn graphs.Genome Res18821829
- 45. Weinstock GM, Norris SJ, Sodergren E, Smajs D (2000) Identification of virulence genes in silico: infectious disease genomics. In: Brogden KA, Roth JA, Stanton TB, Bolin CA, Minion FC, Wannemuehler MJ, editors. Virulence mechanisms of bacterial pathogens. Washington, DC: ASM Press. pp. 251–261.GM WeinstockSJ NorrisE. SodergrenD. Smajs2000Identification of virulence genes in silico: infectious disease genomics.KA BrogdenJA RothTB StantonCA BolinFC MinionMJ WannemuehlerVirulence mechanisms of bacterial pathogensWashington, DCASM Press251261
- 46. Nelson KE, Weinstock GM, Highlander SK, Worley KC, Creasy HH, et al. (2010) A Catalog of Reference Genomes from the Human Microbiome. Science 328: 994–999.KE NelsonGM WeinstockSK HighlanderKC WorleyHH Creasy2010A Catalog of Reference Genomes from the Human Microbiome.Science328994999
- 47. Delcher A, Harmon D, Kasif S, White O, Salzberg SL (1999) Improved microbial gene identification with GLIMMER. Nucleic Acids Res 27: 4636–4641.A. DelcherD. HarmonS. KasifO. WhiteSL Salzberg1999Improved microbial gene identification with GLIMMER.Nucleic Acids Res2746364641
- 48. Lukashin AV, Borodovsky M (1998) GeneMark.hmm: new solutions for gene finding. Nucleic Acids Res 26: 1107–1115.AV LukashinM. Borodovsky1998GeneMark.hmm: new solutions for gene finding.Nucleic Acids Res2611071115
- 49. Gordon D, Abajian C, Green P (1998) Consed: A graphical tool for sequence finishing. Genome Res 8: 195–202.D. GordonC. AbajianP. Green1998Consed: A graphical tool for sequence finishing.Genome Res8195202
- 50. Librado P, Rozas J (2009) DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 1451–1452.P. LibradoJ. Rozas2009DnaSP v5: A software for comprehensive analysis of DNA polymorphism data.Bioinformatics2514511452
- 51. Nei M, Kumar S (2000) Molecular Evolution and Phylogenetics. New York: Oxford University Press. M. NeiS. Kumar2000Molecular Evolution and Phylogenetics.New YorkOxford University Press(333 pp). (333 pp).
- 52. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596–1599.K. TamuraJ. DudleyM. NeiS. Kumar2007MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.Mol Biol Evol2415961599