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Functional Interactions between Retinoblastoma and c-MYC in a Mouse Model of Hepatocellular Carcinoma

  • Louis A. Saddic,

    Affiliations Department of Pediatrics, Stanford University, Stanford, California, United States of America, Department of Genetics, Stanford University, Stanford, California, United States of America

  • Stacey Wirt,

    Affiliations Department of Pediatrics, Stanford University, Stanford, California, United States of America, Department of Genetics, Stanford University, Stanford, California, United States of America

  • Hannes Vogel,

    Affiliation Department of Pathology, Stanford University, Stanford, California, United States of America

  • Dean W. Felsher,

    Affiliations Department of Pathology, Stanford University, Stanford, California, United States of America, Department of Medicine, Stanford University, Stanford, California, United States of America

  • Julien Sage

    Affiliations Department of Pediatrics, Stanford University, Stanford, California, United States of America, Department of Genetics, Stanford University, Stanford, California, United States of America

Functional Interactions between Retinoblastoma and c-MYC in a Mouse Model of Hepatocellular Carcinoma

  • Louis A. Saddic, 
  • Stacey Wirt, 
  • Hannes Vogel, 
  • Dean W. Felsher, 
  • Julien Sage


Inactivation of the RB tumor suppressor and activation of the MYC family of oncogenes are frequent events in a large spectrum of human cancers. Loss of RB function and MYC activation are thought to control both overlapping and distinct cellular processes during cell cycle progression. However, how these two major cancer genes functionally interact during tumorigenesis is still unclear. Here, we sought to test whether loss of RB function would affect cancer development in a mouse model of c-MYC-induced hepatocellular carcinoma (HCC), a deadly cancer type in which RB is frequently inactivated and c-MYC often activated. We found that RB inactivation has minimal effects on the cell cycle, cell death, and differentiation features of liver tumors driven by increased levels of c-MYC. However, combined loss of RB and activation of c-MYC led to an increase in polyploidy in mature hepatocytes before the development of tumors. There was a trend for decreased survival in double mutant animals compared to mice developing c-MYC-induced tumors. Thus, loss of RB function does not provide a proliferative advantage to c-MYC-expressing HCC cells but the RB and c-MYC pathways may cooperate to control the polyploidy of mature hepatocytes.


Cancer is a complex disease that often progresses slowly due to the gradual accumulation of genetic and epigenetic alterations over time [1], [2]. Typically, tumor cells harbor mutations that activate oncogenes and inactivate tumor suppressors. The combination of these alterations promotes deregulated cell division, one of the hallmarks of the cancer phenotype [1]. Despite this universal property of tumors, many outstanding questions remain, including whether the order of the successive alterations is critical to cellular transformation and how mutations in cancer pathways cooperate in the course of the disease.

The Retinoblastoma protein (RB) is a potent tumor suppressor that restricts S phase entry by inhibiting the activity of the E2F family of transcription factors [3]. Early in G1, activation of Cyclin/CDK complexes by mitogenic signals results in RB phosphorylation and functional inactivation, thus allowing E2F family members to transcribe genes necessary for cell cycle progression [4]. In addition to this well-described function of RB, emerging evidence indicates that RB also normally promotes differentiation in multiple lineages [5], [6], [7], [8], [9], [10], [11] and protects cells from the accumulation of genomic alterations [12], [13], [14], [15], [16], [17]. Due to the critical influence of RB in the control of cell cycle progression, it is not surprising that RB or members of the RB pathway are mutated in nearly all human cancers [18], [19].

c-MYC (hereafter referred to as MYC) is a transcription factor that heterodimerizes with its partner MAX in order to control the expression of a large program of genes that promote proliferation, cell death, cell growth, and cellular differentiation [20], [21], [22], [23]. In resting cells, MYC activity is often minimal because of low mRNA and protein levels; in contrast, MYC activity is strongly induced in tumor cells by multiple mechanisms, including increased transcription, stabilization of the protein, gene amplification, and chromosomal translocation [24], [25]. MYC activation is a common feature of many human cancers, including cancers with mutations in the RB pathway [20], [21], [22], [26].

Hepatocellular carcinoma (HCC) is the third most common cause of cancer death in the world with more than 500,000 deaths a year [27]; the number of HCC cases increases every year [28]. While several causal agents for HCC have been identified, including infection with hepatitis B and C viruses (HBV and HCV), there is no effective treatment for this cancer type, in part because the molecular and cellular mechanisms of HCC development are still poorly understood [29], [30], [31]. MYC is amplified in up to 50% of HCC cases, suggesting a key role for MYC activation in the development of these tumors [32], [33], [34]. Similarly, inactivation of the RB pathway is found in more than two-thirds of human HCCs by several mechanisms, including inhibition of p16INK4a and its family member p15INK4b, increased expression of Cyclin D1, and loss of RB function by phosphorylation, protein degradation, or gene mutation [35], [36].

Mouse models carrying mutations commonly found in human tumors provide an opportunity to investigate the mechanisms of tumorigenesis in vivo. A mouse model of human HCC with inducible expression of MYC in adult liver cells has shown that overexpression of MYC is sufficient to initiate HCC development; however, these tumors develop with a prolonged latency, suggesting that other genetic alterations are necessary to generate HCC, including mutations in the p53 pathway [32], [37], [38], [39], [40]. The frequency of MYC activation and loss of RB pathway function in human HCC suggests that these two pathways may interact during tumorigenesis in the liver. However, whether or not inactivation of RB can cooperate with MYC in the formation of murine HCC has yet to be demonstrated. In this report, we use a mouse model that allows us to conditionally delete the Rb gene and overexpress MYC specifically in the liver. We show that loss of RB has minimal effects on the development of HCC initiated by the overexpression of MYC, suggesting that these two cancer genes share many functions in liver cells undergoing tumorigenic transformation.


Combined activation of MYC and inactivation of RB in the liver of adult mice results in the development of hepatocellular carcinoma

To investigate the potential interactions between MYC overexpression and RB loss of function in HCC, we bred conditional mutant Rblox/lox mice [41] with LAP-tTA TRE-MYC mice, in which expression of a human MYC cDNA can be induced specifically in the liver [32], [42] (Figure 1A). We found that, as previously shown [32], [42], expression of MYC in the liver of adult mice (“MYC mutant mice”) resulted in the development of liver tumors. We used intrasplenic injection of an adenoviral vector expressing the Cre recombinase (Ad-Cre) to specifically delete Rb in the adult liver and found that loss of Rb was not sufficient to initiate liver cancer development, as previously shown [43], [44] (data not shown, see below). MYC/Rb double mutant mice developed liver tumors composed of multiple fleshy vascular nodules resembling MYC mutant tumors (data not shown). Total RNA from tumors macro-dissected at the surface of the liver showed high levels of expression of MYC compared to control liver samples, independent of the presence or the absence of Rb, as expected (Figure 1B). Immunoblot analysis of liver extracts revealed high levels of the MYC protein in all tumor samples compared to controls (Figure 1C). PCR analysis of genomic DNA isolated from tumors showed deletion of Rb in all the double mutant tumors examined (n = 6); no Rb deletion was observed in mice expressing the MYC transgene and infected with an Ad-GFP control adenovirus (n = 6) (Figure 1D). Quantitative RT-PCR (RT-qPCR) analysis of total RNA extracted from MYC/Rb double mutant tumors showed a decrease in Rb levels compared to MYC mutant tumors (Figure 1E). These data showed that deletion of Rb in the liver of the infected mice was efficient and not counter-selected during the development of HCC initiated by activation of MYC.

Figure 1. MYC activation and Rb deletion drive HCC development in the liver of adult mice.

A. Strategy to produce MYC and MYC/Rb mutant tumors using Rblox/lox and TRE-MYC LAP-tTA mice. MYC is activated in the liver by the removal of doxycycline from the drinking water while Rb is specifically deleted in the liver by splenic injection of Ad-Cre. Ad-GFP is used as a control. B. RT-qPCR analysis of MYC RNA levels in control livers (Rblox/lox MYCOff, n = 2) and in MYC and MYC/Rb mutant tumors (n = 3). C. Immunoblot analysis of MYC protein levels in control livers (MYCOff) and in MYC and MYC/Rb mutant tumors (two independent tumors each, T1 and T2). Actin serves as a loading control. D. Genomic PCR analysis for the deleted allele of Rb (RbΔ) using DNA from MYC and MYC/Rb mutant tumors. Actin serves as a positive PCR control. E. RT-qPCR analysis of Rb RNA levels in MYC (black, n = 3) and MYC/Rb (grey, n = 3) mutant tumors.

When we analyzed livers from MYC and MYC/Rb mutant mice, we found that tumors from both genotypes were composed of hepatocellular neoplasms characterized by sheets of cells with occasional mitotic figures, slightly pleomorphic nuclei, and prominent nucleoli (Figure 2A). Examples from each genotype showed a range of differentiation between well- and moderately-differentiated HCC without an obvious tendency in the presence of either genotype. In addition, tumors from both genotypes expressed levels of Albumin, a marker of hepatocytes, that were lower than those found in control livers. Levels of Cytokeratin 19 (CK19), a marker of cholangiocytes and some liver progenitors [45], were not significantly different between control livers and the tumors. Levels of Afp were increased in tumors, similar to what is commonly observed in human HCC [46] (Figure 2B). These observations indicate that loss of Rb in this mouse model of HCC induced by expression of MYC does not grossly affect the histopathological features of these tumors, allowing us to further investigate how loss of RB and MYC overexpression may function during HCC development.

Figure 2. Rb inactivation does not change the histology and differentiation status of MYC-induced HCC.

A. Histology of non-tumor liver tissue from MYC mutant mice (left) and tumor tissue from MYC (center) and MYC/Rb (right) mutant mice. B. RT-qPCR analysis of Albumin (a marker of hepatocytes), CK19 (a marker of bile ducts), and Afp (a marker of HCC) in control livers (white, n = 2), MYC mutant tumors (black, n = 3), and MYC/Rb mutant tumors (grey, n = 3). Note that the p-value for Afp between control livers and MYC/Rb mutant tumors is 0.06, just below the significance threshold.

Activation of MYC and loss of RB do not cooperate to control cell cycle progression in the liver of adult mice

Immunofluorescence analysis for Ki67 expression, a marker of cycling cells, and for BrdU incorporation, a marker of DNA replication, showed that tumors from both genotypes displayed high indices of proliferation that were not visibly different (Figure 3A–B and D). MYC and MYC/Rb mutant tumors also showed grossly similar levels of apoptotic cell death as detected by immunofluorescence for cleaved caspase 3 (CC3) (Figure 3C–D). To corroborate these observations made on tumor sections, we next measured mRNA and protein expression levels of cell cycle genes from dissected tumors. We did not detect any significant differences between MYC and MYC/Rb mutant tumors, which both expressed high levels of these cell cycle regulators compared to wild-type livers (Figure 4A–B).

Figure 3. Rb inactivation does not affect the degree of proliferation or cell death of MYC-induced HCC.

A–C. Immunofluorescence staining for Ki67 (A), BrdU (B), and cleaved caspase 3 (CC3) (C) on MYC mutant non-tumor liver tissue and tumor tissue from MYC and MYC/Rb mutant mice. DAPI nuclear staining is used to indicate the density of cells on the sections. The pictures shown are representative of each group. D. Quantification of staining shown in A. The number of positively stained cells for each antibody over the number of DAPI stained cells expressed as a percent was determined using the BioQuant software. For each antibody and genotype combination the average of n = 2 mice was calculated where each n is the average percent of positive cells from three fields containing at least 250 cells each.

Figure 4. Rb inactivation does not change the mRNA expression or protein levels of cell cycle markers in MYC-induced HCC.

A. RT-qPCR for several cell cycle genes on RNA from MYC (black, n = 3) and MYC/Rb (grey, n = 3) mutant HCC. Rblox/lox MYCOff non-tumor liver is used as a control (CTRL) (white, n = 2). There is not statistical difference for any of these gene expression levels between MYC and MYC/Rb tumors. B. Representative immunoblot analysis for several cell cycle markers on protein extracts from MYC and MYC/Rb mutant HCC compared to wild-type liver. The concentration of the protein extracts was quantified using a Bradford assay and similar amounts of proteins were loaded in each lane. Actin serves as a loading control; note that CTRL liver cells express less Actin per µg of protein extract than tumor cells.

These observations indicated that MYC and MYC/Rb mutant HCCs were very similar but did not exclude that the early stages of cancer development may have different characteristics. To begin to investigate the features of liver cancer initiation in mice from both genotypes, LAP-tTA TRE-MYC mice were bred to Rosa26+/CreER Rblox/lox mice, in which recombination of alleles flanked by lox sites can be triggered by administration of intraperitoneal injections of tamoxifen. We found that this approach gave less variable results than the adenoviral infection at early time points (data not shown) and resulted in the efficient deletion of Rb (Figure 5A).

Figure 5. Rb inactivation and MYC activation cooperate to increase ploidy in hepatocytes.

A. Genomic PCR analysis for the deleted allele of Rb (RbΔ) using DNA from control, MYC, Rb, and MYC/Rb mutant livers before the development of tumors in Rosa26+/CreER mice. Actin serves as a positive PCR control. B. Quantification of immunostaining experiments for the number of Ki67+ liver cells per field on control, MYC, Rb, and MYC/Rb mutant liver sections 5 weeks after the deletion of Rb and the induction of MYC. The number of positive cells in eight 20× fields was counted in 2 mice for each treatment group. The differences observed are not significant in a t-test. C. Representative microphotographs of hepatocytes from a mouse injected with corn oil alone in the presence of doxycycline (wild-type for RB and MYC) and a mouse injected with tamoxifen in the absence of doxycycline are shown (MYC/Rb mutant liver). Arrows show a small nucleus in the control mouse and a large nucleus in the mutant mouse. D. Quantification of ploidy by FACS of hepatocyte populations from control (n = 5), MYC (n = 8), Rb (n = 5), and MYC/Rb (n = 8) mutant livers 5 months after Rb deletion (Rosa26+/CreER mice) and MYC activation. Only statistically significant differences are shown.

Previous studies have shown that activation of the MYC transgene in the liver of adult mice does not change the proliferation of hepatocytes compared to wild-type livers [32], [42]. In contrast, deletion of Rb is not sufficient to initiate liver cancer development but has been shown to cause widespread cell cycle-entry in hepatocytes [44]. We observed that, before any tumor lesions could be observed histopathologically, Rb mutant mice displayed elevated Ki67 staining on liver sections compared to Rb wild-type mice; activation of the MYC transgene did not induce proliferation in populations of adult hepatocytes, as expected, and slightly increased the number of Ki67+ cells in Rb deficient mice, but this trend was not significant (Figure 5B).

Together, these experiments indicate that loss of RB does not significantly change the molecular or physical identity of MYC-induced HCC in this mouse model.

Activation of MYC and deletion of Rb together lead to increased polyploidy in adult liver cells

We next investigated the potential effects of MYC activation and Rb deletion on the DNA content of hepatocytes in pre-neoplastic livers. Previous studies have reported that both overexpression of MYC alone and inactivation of RB alone in adult mice leads to an increase in ploidy of hepatocytes [44], [47], [48], [49], [50]. The histopathological analysis of liver sections from Rb and MYC/Rb mice after 5 months of induction of the MYC transgene and deletion of Rb showed accumulation of hepatocytes with large nuclei compared to control mice, before the appearance of tumors (Figure 5C).

FACS analysis (Figure 5D) showed that activation of MYC resulted in a non-significant increase in ploidy in hepatocytes after 5 months in this system, presumably because full MYC stabilization and activation is only effective once tumorigenesis has been initiated in cells [32], [39], [42] (DWF, unpublished observations). Loss of RB led to decreased numbers of 4n hepatocytes and a concomitant increase of 8n and 16n cells, consistent with increased polyploidy. Activation of MYC in Rb deficient mice also generated significantly more 4n hepatocytes compared to loss of RB alone. MYC/Rb double mutant mice showed a significant increase in ploidy compared to MYC mutant mice, with fewer 2n populations and more 8n and 16n populations (Figure 5D). These observations suggest that loss of RB may be more influential than activation of MYC in perturbing DNA content in adult hepatocytes, but also that the two genetic events cooperate to control endoreduplication of mature hepatocytes under these experimental conditions.

Rb/MYC double mutant mice die from HCC faster than MYC mutant mice

MYC and MYC/Rb mutant mice develop tumors that are histopathologically similar and express comparable levels of cell cycle regulators. However, it was still possible that loss of RB function and activation of MYC may cooperate during tumor development; in particular, MYC/Rb mutant mice have more polyploid hepatocytes compared to MYC mutant mice before tumors are detectable (Figure 5D) and polyploidy has been associated with cancer, including liver cancer [51], [52]. We tested this possibility by monitoring the survival of MYC and MYC/Rb mutant mice over a year after activation of MYC and deletion of Rb. As reported before, not all MYC mutant mice develop HCC when the transgene is induced in adult mice [32], [42]. Indeed, we found that some mutant mice were still alive at 52 weeks and that none of these mice died from HCC between 52 and 75 weeks. Because mice tend to develop tumors naturally after one year of age, including some liver lesions, we focused our analysis on mice aged one year after the removal of doxycycline. Importantly, none of the control mice (wild-type for MYC and wild-type or mutant for Rb, n = 12) died from liver cancer within this first year (data not shown).

We found that loss of Rb did not change the number of mice that do not develop HCC within one year, suggesting that Rb deletion does not affect cancer initiation and confirming that tumorigenesis is driven by activation of MYC in this mouse model (Figure 6A). MYC mutant mice had a median survival of 31 weeks while the median survival of MYC/Rb mutant mice was 27 weeks; when the mice that did not die from liver cancer were excluded from the analysis, the median survival for MYC and MYC/Rb mutant mice decreased to 27 and 16.5 weeks, respectively, with a P value of 0.07 in a Gehan-Breslow-Wilcoxon test. Thus, while not statistically significant, this analysis is indicative of a trend that MYC/Rb mutant mice die faster from HCC than mice with MYC activation alone, suggesting that loss of Rb may cooperate with activation of MYC in HCC.

Figure 6. Survival analysis of Myc and Myc/Rb mutant mice.

A. Kaplan-Meier survival analysis of MYC (n = 27) and MYC/Rb (n = 19) mutant mice when both tumorigenic events were triggered simultaneously (time 0) in adult mice (8–12 weeks after birth) by removal of doxycycline and Ad-Cre infection. B. Survival analysis of MYC/Rb mutant mice developing HCC when both tumorigenic events were triggered simultaneously in adult mice (8–12 weeks after birth) (n = 19) and when Rb deletion by Ad-Cre was performed 4 weeks before MYC activation (8–12 weeks and 12–16 weeks after birth, respectively) (n = 15); time 0 is the time of MYC activation.

The possibility to control MYC activation and Rb deletion with two different methods (removal of doxycycline and Cre-mediated recombination, respectively) also allowed us to test the possibility that the order of the mutations in this system may influence tumor development. While deletion of Rb is not sufficient to initiate cancer, it results in increased proliferation and ploidy. We hypothesized that activation of MYC in this context may facilitate and accelerate tumor development in the liver. To test this idea, we infected mice with Ad-Cre and then, one month later, activated MYC by removing doxycycline from the water. The analysis of these two cohorts of MYC/Rb mutant mice showed no difference in survival curves when survival was measured starting at the time of MYC activation (Figure 6B). Thus, in this context, pre-deletion of Rb does not affect tumor development in the liver of mice expressing MYC.


In this study we investigated if loss of RB function may cooperate with activation of MYC in a mouse model of HCC. While RB and MYC are important players in the tumorigenic process, this is the first time, to our knowledge, that their functional interactions have been tested genetically in vivo. We found that MYC and MYC/Rb mutant tumors closely resembled each other, including similar appearances grossly, histologically, and molecularly. The only identifiable difference was the degree of ploidy in pre-neoplastic tissue where MYC/Rb mutant pre-tumor tissue displayed a higher degree of ploidy than MYC mutant pre-tumor tissue in hepatocyte populations.

Several oncogenes and growth factors, such as E2F1 and TGFα, have been shown to cooperate with MYC in mouse models of HCC development [53], [54], [55], [56]. Clearly, tumors induced by oncogenes such as MYC must have some levels of inactivation of the RB pathway to be able to grow. However, it is surprising that we did not observe a strong enhancement of HCC development when Rb deletion is added to MYC activation, especially given the observation that many human samples of HCC tend to harbor mutations that simultaneously activate MYC and inactivate the RB pathway [57], [58]. There are a few possibilities that could explain this observation. For example, MYC has been shown to directly induce transcription of Cyclin D2 and Cdk4, which in turn leads to the sequestration of p27 away from Cyclin E and thus the accumulation of Cyclin E/Cdk2 complexes; these kinases then lead to the phosphorylation and inactivation of RB and its family members p107 and p130 [20], [21], [22]. In fact, inactivation of MYC delays phosphorylation of RB after growth stimulation [59]. Moreover, MYC induces the expression of miRNA genes that can repress the expression levels of Rb and other cell cycle inhibitors [60]. MYC can also directly activate genes downstream of RB/E2F complexes [61], [62], [63]. As a result, it is possible that MYC activation alone is enough to critically inactive the RB pathway. In support of this hypothesis, we found that cells expressing high levels of MYC also express lower levels of Rb mRNA compared to control hepatocytes (data not shown). However, as mentioned above, the observation that activation of MYC and loss RB function are selected for in many cancers, including HCC [20], [21], [22], [26], would argue against the idea that activation of MYC is sufficient to inactivate RB function and that loss of both RB alleles is merely a passenger effect.

Another possibility to explain the absence of strong cooperation between activation of MYC and deletion of Rb in our mouse HCC model is that mouse tumors driven by MYC overexpression develop by different mechanisms than human tumors expressing high levels of MYC, which would make loss of RB function dispensable. Thus, it will be interesting in the future to examine the consequences of deleting Rb in mice in which MYC activation is achieved using different systems, including different liver promoters and at different time points during tumorigenesis.

It is also possible that, similar to what has been observed in the eye of mice [64], the RB family members p107 and p130 have a stronger overlapping role in the mouse liver compared to the human liver. The idea that inactivation of several RB family members may be required to observe a strong phenotype during HCC development is supported by the observation that most of the events known to inactivate the RB pathway are upstream events and may inactivate RB, p107, and p130 simultaneously [65], [66], [67], [68], [69], [70], [71], [72], [73]. Future experiments may examine HCC development in MYC/Rb mutant mice in which p107 and or p130 are also inactivated, as well as the tumor phenotypes of mice in which Rb is deleted and MYC is activated in other tissues and organs. In fact, we have previously conducted a similar study examining the interaction between oncogenic RAS and loss of Rb in a mouse model of lung adenocarcinoma. In this model, RAS-induced tumors also showed partial inactivation of RB function, but loss of Rb had two effects: initially it increased cancer initiation and then led to decreased proliferation, presumably due to compensation by p107 and p130 [74]. As a result it appears that the effects of cooperation between an oncogene and a tumor suppressor may not be reliably predicted when based solely on the degree of overlap between their pathways.

Recent observations indicate that the timing of p53 re-expression during lung cancer development may affect cancer outcome [75], [76]. The system we employed utilizes the tetracycline-dependent regulatory system to activate MYC and the Cre-lox conditional mutation system to delete Rb. This enabled us to investigate the consequences of changing the time of the tumorigenic events. We hypothesized that prior mutation in the Rb gene may facilitate MYC-induced tumor development in the liver but found no differences in HCC development whether Rb was deleted before MYC was activated or at the same time. We also found that deletion of Rb one month after activation of MYC did not impact survival (data not shown). These negative results may be due to the fact that, as discussed above, loss of RB may not strongly modify the tumor phenotype of MYC-expressing mice in our model. Evidence from HCC patients suggests that RB loss is a late event in HCC development [66], and it is possible that a similar strategy in a different mouse model may identify specific functions for RB during HCC progression, including during metastasis [66].

One such function for RB may be to protect genome integrity. Recent evidence indicates that, in addition to its role at the G1/S transition of the cell cycle, RB may also play a critical role to prevent chromosomal instability [77], [78], [79], [80]. While the role of polyploidy in cancer development is still controversial, increasing evidence suggests that it may result in aneuploidy, which may contribute to cancer initiation [51], [52], [81], [82]. We found that loss of RB may increase polyploidy in MYC-expressing hepatocytes. Recent evidence also suggests that one mechanism of HCC development upon infection with hepatitis C virus (HCV) may be through increased genomic instability following inactivation of RB [48]. One limitation of our study is that the cell of origin of the liver tumors developing upon ectopic expression of MYC is unknown, thereby limiting any further investigation of the potential role of increased polyploidy in HCC development. It will be interesting in the future to determine in other cancer models if activation of MYC and loss of RB cooperate in HCC development by altering chromosome numbers.

Materials and Methods


All animal work was approved by Stanford IACUC committee (protocol number 13565) and follows AAALAC guidelines. TRE-MYC LAP-tTA mice were bred to either conditional Rblox/lox or Rblox/lox Rosa26-CreER mice. The human MYC transgene was activated by the removal of doxycycline (100 µg/ml) from the drinking water. Recombination of alleles in Rblox/lox mice was accomplished by splenic injection of 5×108 pfu Ad-Cre (Vector Development Laboratory, Baylor). Control mice were treated with equal amounts of AdGFP (Baylor College of Medicine). The Cre recombinase was induced in Rblox/lox Rosa26-CreER mice by five consecutive daily injections of 1 mg of tamoxifen (Sigma) in corn oil; control mice were injected with corn oil alone [83]. All treatments were performed on adult mice (8–12 weeks old). Deletion of the Rb gene was monitored by genomic PCR, as described before [83].

RNA analysis

RNA was extracted with TRIzol (Invitrogen) and cleaned with the RNeasy Mini Kit (QIAGEN). RT-PCR and quantitative Real-Time PCR were performed using the DyNAmo cDNA synthesis kit and the SybrGreenER Mastermix (Invitrogen), respectively. Primer Sequences: Rb delta forward 5′- GGAGAAAGTTTCATCCGTGGAT -3′ reverse 5′- GTGAATGGCATCTCATCTAGATCAA -3′; human MYC forward 5′-TGCTCCATGAGGAGACACC-3′ reverse 5′-CCTCATCTTCTTGTTCCTCCA-3′; p107 forward 5′- CCGAAGCCCTGGATGACTT-3′ reverse 5′- GCATGCCAGCCAGTGTATAACTT-3′; p130 forward 5′- TGTCCGGCCTCAGGAATG-3′ reverse 5′- CTGTCAGCGATAGCCTGAGTTG-3′; p53 forward 5′- GCCCATGCTACAGAGGAGTC-3′ reverse 5′- AGACTGGCCCTTCTTGGTCT-3′; p21 forward 5′-CAGATCCACAGCGATATCCA-3′ reverse 5′-GGCACACTTGCTCCTGTG-3′; E2f1 forward 5′-TGCCAAGAAGTCCAAGAATCA-3′ reverse 5′-CTTCAAGCCGCTTACCAATC-3′; Cyclin E1 forward 5′- CTGAGAGATGAGCACTTTCTGC-3′ reverse 5′- GAGCTTATAGACTTCGCACACCT-3′; B-myb forward 5′-TTACGCCGTACGTGGAAGA-3′ reverse 5′-TTCCAGTCTTGCTGTCCAAA-3′; cdc25c forward 5′- GGAAACACCCGGATCTGAA-3′ reverse 5′- ACTTTCCAGACAGCAAAGCAG-3′; Cyclin A2 forward 5′-CTTGGCTGCCACCAACAGTAA-3′ reverse 5′-CAAACTCAGTTCTCCCAAAAACA-3′; GAPDH forward 5′-GGGTTCCTATAAATACGGACTGC-3′ reverse 5′-CCATTTTGTCTACGGGACGA-3′; Afp forward 5′- TGCTGCAAAGCTGAAAATGC -3′ reverse 5′- GCTGCTTTCTCTTAATTCTTTTGTAACTG -3′; Albumin forward 5′-AGTGTTGTGCAGAGGCTGAC-3′ reverse 5′-TTCTCCTTCACACCATCAAGC-3′; CK19 forward 5′-TGACCTTGGAGATGCAGATTG-3′ reverse 5′-CCTCAGGGCAGTAATTTCCTC-3′.

Immunoblot analysis

Whole cell extracts from tissue were prepared in 50 µM Tris pH 7.4, 250 µM NaCl, 10% glycerol, 0.5% Triton-X-100, and a cocktail of protease inhibitors. 75 µg of protein extract were run on acrylamide gels and transferred to a PVDF membrane (Hybond). Membranes were probed with the following antibodies: CDK2 (Santa Cruz Biotechnology, sc-163-G), Cyclin E1 (Santa Cruz Biotechnology, sc-198), E2F1 (Santa Cruz Biotechnology, sc-193X), p107 (Santa Cruz Biotechnology, sc-318), p53 (Vector Laboratories, CM5), PCNA (Santa Cruz Biotechnology, PC10), and β-Actin (Sigma, A5441). Secondary antibodies conjugated to HRP (Jackson ImmunoResearch) were used at a concentration of 1∶5000, and signal was detected by ECL (Invitrogen).

Histology and Immunostaining

Mouse organs were fixed in 4% paraformaldehyde overnight and embedded in paraffin. Sections were stained with hematoxylin and eosin (H&E). The histopathological analysis was performed blind by a trained pathologist (H.V.). Mice were injected with 1 mg of BrdU (Sigma) 4 hours prior to dissection. Sections were dewaxed and rehydrated in Trilogy buffer (Cell Marque) in a pressure cooker for 15 minutes, blocked in 5% serum for 30 min, and incubated with primary antibody overnight at 4°C. The next day, sections were incubated with secondary antibody for 1 hour, stained with DAPI (for immunofluorescence) or hematoxylin (for immunohistochemistry), and mounted. Antibodies used include Ki67 (Becton-Dickinson, 550609), BrdU (Becton-Dickinson, 347580), and Cleaved Caspase-3 (CC3, Cell Signaling, 96645). Quantification of cell cycle and cell death was performed using the BioQuant imaging analysis software.

Flow cytometry

Hepatocyte nuclei were extracted by grinding murine liver in Lysis Buffer (25 mM Tris pH 7.5, 50 mM KCl, 2 mM MgCl2, 1 mM EDTA, and 1 mM PMSF), followed by homogenization and centrifugation at 12,000 rpm for 30 seconds [49]. Pellets were then resuspended in Lysis Buffer and centrifuged again at 12,000 rpm for 30 seconds. These pellets were then resuspended in PI Buffer (0.5 mg/ml Propidium Iodide, 0.1% NP-40, 0.1% Sodium Citrate, 40 µg/ml RNase A, in PBS) for 30 min. FACS analysis was performed with a BD FACSCalibur instrument and data was analyzed using the FlowJo software (Tree Star).

Statistical analyses

Statistical significance was assayed by Student's t-test using the GraphPad Prism software, except for Figure 5D where ANOVA was used to compare the four genotypes for each ploidy analyzed and for Figure 6 where the Gehan-Breslow-Wilcoxon test was used to compare the survival of mouse cohorts. Mean and standard error are shown. ns, not statistically significant, *, p<0.05 - ** p<0.005 - ***, p<0.001.


The authors thank Dr. Erik Knudsen for his help with the ploidy measurement protocol, and members of the Sage laboratory for helpful discussions.

Author Contributions

Conceived and designed the experiments: LAS DWF JS. Performed the experiments: LAS SW. Analyzed the data: LAS SW HV DWF JS. Contributed reagents/materials/analysis tools: DWF. Wrote the paper: LAS SW JS.


  1. 1. Hanahan D, Weinberg RA (2000) The Hallmarks of Cancer. Cell 100: 57–70.
  2. 2. Lund AH, van Lohuizen M (2004) Epigenetics and cancer. Genes Dev 18: 2315–2335.
  3. 3. Weinberg RA (1995) The retinoblastoma protein and cell cycle control. Cell 81: 323–330.
  4. 4. Sherr CJ, McCormick F (2002) The RB and p53 pathways in cancer. Cancer Cell 2: 103–112.
  5. 5. Deshpande A, Hinds PW (2006) The retinoblastoma protein in osteoblast differentiation and osteosarcoma. Curr Mol Med 6: 809–817.
  6. 6. Skapek SX, Pan YR, Lee EY (2006) Regulation of cell lineage specification by the retinoblastoma tumor suppressor. Oncogene 25: 5268–5276.
  7. 7. Khidr L, Chen PL (2006) RB, the conductor that orchestrates life, death and differentiation. Oncogene 25: 5210–5219.
  8. 8. Nguyen DX, McCance DJ (2005) Role of the retinoblastoma tumor suppressor protein in cellular differentiation. J Cell Biochem 94: 870–879.
  9. 9. Thomas DM, Yang HS, Alexander K, Hinds PW (2003) Role of the retinoblastoma protein in differentiation and senescence. Cancer Biol Ther 2: 124–130.
  10. 10. Classon M, Harlow E (2002) The retinoblastoma tumour suppressor in development and cancer. Nature Rev Cancer 2: 910–917.
  11. 11. Iavarone A (2004) Retinoblastoma promotes definitive erythropoiesis by repressing Id2 in fetal liver macrophages. Nature 432: 1040–1045.
  12. 12. Zheng L, Flesken-Nikitin A, Chen PL, Lee WH (2002) Deficiency of Retinoblastoma gene in mouse embryonic stem cells leads to genetic instability. Cancer Res 62: 2498–2502.
  13. 13. Zheng L, Lee WH (2002) Retinoblastoma tumor suppressor and genome stability. Adv Cancer Res 85: 13–50.
  14. 14. Srinivasan SV, Mayhew CN, Schwemberger S, Zagorski W, Knudsen ES (2007) RB loss promotes aberrant ploidy by deregulating levels and activity of DNA replication factors. J Biol Chem 282: 23867–23877.
  15. 15. Hernando E (2004) Rb inactivation promotes genomic instability by uncoupling cell cycle progression from mitotic control. Nature 430: 797–802.
  16. 16. Knudsen ES, Sexton CR, Mayhew CN (2006) Role of the retinoblastoma tumor suppressor in the maintenance of genome integrity. Curr Mol Med 6: 749–757.
  17. 17. Harrington EA, Bruce JL, Harlow E, Dyson N (1998) pRB plays an essential role in cell cycle arrest induced by DNA damage. Proc Natl Acad Sci USA 95: 11945–11950.
  18. 18. Burkhart DL, Sage J (2008) Cellular mechanisms of tumour suppression by the retinoblastoma gene. Nat Rev Cancer 8: 671–682.
  19. 19. Knudsen ES, Knudsen KE (2008) Tailoring to RB: tumour suppressor status and therapeutic response. Nat Rev Cancer 8: 714–724.
  20. 20. Grandori C, Cowley SM, James LP, Eisenman RN (2000) The Myc/Max/Mad network and the transcriptional control of cell behavior. Annu Rev Cell Dev Biol 16: 653–699.
  21. 21. Oster SK, Ho CS, Soucie EL, Penn LZ (2002) The myc oncogene: MarvelouslY Complex. Adv Cancer Res 84: 81–154.
  22. 22. Pelengaris S, Khan M, Evan G (2002) c-MYC: more than just a matter of life and death. Nat Rev Cancer 2: 764–776.
  23. 23. Dang CV (1999) c?Myc target genes involved in cell growth, apoptosis, and metabolism. Mol Cell Biol 19: 1–11.
  24. 24. Pelengaris S, Khan M, Evan G (2002) c-MYC: more than just a matter of life and death. Nat Rev Cancer 2: 764–776.
  25. 25. Meyer N, Penn LZ (2008) Reflecting on 25 years with MYC. Nat Rev Cancer 8: 976–990.
  26. 26. Felsher DW, Bishop JM (1999) Transient excess of MYC activity can elicit genomic instability and tumorigenesis. Proc Natl Acad Sci USA 96: 3940–3944.
  27. 27. Caldwell S, Park SH (2009) The epidemiology of hepatocellular cancer: from the perspectives of public health problem to tumor biology. J Gastroenterol 44(Suppl 19): 96–101.
  28. 28. McMahon BJ (2009) The natural history of chronic hepatitis B virus infection. Hepatology 49: S45–55.
  29. 29. Farazi PA, DePinho RA (2006) Hepatocellular carcinoma pathogenesis: from genes to environment. Nat Rev Cancer 6: 674–687.
  30. 30. Thomas MB, Jaffe D, Choti MM, Belghiti J, Curley S, et al. (2010) Hepatocellular carcinoma: consensus recommendations of the National Cancer Institute Clinical Trials Planning Meeting. J Clin Oncol 28: 3994–4005.
  31. 31. Sherman M (2010) Hepatocellular carcinoma: epidemiology, surveillance, and diagnosis. Semin Liver Dis 30: 3–16.
  32. 32. Beer S, Zetterberg A, Ihrie RA, McTaggart RA, Yang Q, et al. (2004) Developmental Context Determines Latency of MYC-Induced Tumorigenesis. PLoS Biol 2: e332.
  33. 33. Abou-Elella AGT, Fritsch C, Gansler T (1996) c-myc amplification in hepatocellular carcinoma predicts unfavorable prognosis. Modern Pathology 9: 95–98.
  34. 34. Kawate S, Fukusato T, Ohwada S, Watanuki A, Morishita Y (1999) Amplification of c-Myc in Hepatocellular Carcinoma: Correlation with Clinicopathologic Features, Proliferative Activity and p53 Overexpression. Oncology 57: 157–163.
  35. 35. Laurent-Puig P, Zucman-Rossi J (2006) Genetics of hepatocellular tumors. Oncogene 25: 3778–3786.
  36. 36. Farazi PA, DePinho RA (2006) Hepatocellular carcinoma pathogenesis: from genes to environment. Nat Rev Cancer 6: 674–687.
  37. 37. Sandgren EPQC, Pinkert CA, Palmiter RD, Brinster RL (1989) Oncogene-induced liver neoplasia in transgenic mice. Oncogene 4: 715–724.
  38. 38. Shachaf CM, Gentles AJ, Elchuri S, Sahoo D, Soen Y, et al. (2008) Genomic and proteomic analysis reveals a threshold level of MYC required for tumor maintenance. Cancer Res 68: 5132–5142.
  39. 39. Wu CH, van Riggelen J, Yetil A, Fan AC, Bachireddy P, et al. (2007) Cellular senescence is an important mechanism of tumor regression upon c-Myc inactivation. Proc Natl Acad Sci U S A 104: 13028–13033.
  40. 40. Giuriato S, Ryeom S, Fan AC, Bachireddy P, Lynch RC, et al. (2006) Sustained regression of tumors upon MYC inactivation requires p53 or thrombospondin-1 to reverse the angiogenic switch. Proc Natl Acad Sci U S A 103: 16266–16271.
  41. 41. Sage J, Miller AL, Perez-Mancera PA, Wysocki JM, Jacks T (2003) Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry. Nature 424: 223–228.
  42. 42. Shachaf CM, Kopelman AM, Arvanitis C, Karlsson A, Beer S, et al. (2004) MYC inactivation uncovers pluripotent differentiation and tumour dormancy in hepatocellular cancer. Nature 431: 1112–1117.
  43. 43. Williams BO, Schmitt EM, Remington L, Bronson RT, Albert DM, et al. (1994) Extensive contribution of Rb-deficient cells to adult chimeric mice with limited histopathological consequences. Embo J 13: 4251–4259.
  44. 44. Mayhew CN, Bosco EE, Fox SR, Okaya T, Tarapore P, et al. (2005) Liver-specific pRB loss results in ectopic cell cycle entry and aberrant ploidy. Cancer Res 65: 4568–4577.
  45. 45. Roskams T (2006) Liver stem cells and their implication in hepatocellular and cholangiocarcinoma. Oncogene 25: 3818–3822.
  46. 46. Yamashita T, Forgues M, Wang W, Kim JW, Ye Q, et al. (2008) EpCAM and alpha-fetoprotein expression defines novel prognostic subtypes of hepatocellular carcinoma. Cancer Res 68: 1451–1461.
  47. 47. Conner EA, Lemmer ER, Sanchez A, Factor VM, Thorgeirsson SS (2003) E2F1 blocks and c-Myc accelerates hepatic ploidy in transgenic mouse models. Biochem Biophys Res Commun 302: 114–120.
  48. 48. Machida K, Liu JC, McNamara G, Levine A, Duan L, et al. (2009) Hepatitis C Virus Causes Uncoupling of Mitotic Checkpoint and Chromosomal Polyploidy through the Rb Pathway. J Virol.
  49. 49. Srinivasan SV, Mayhew CN, Schwemberger S, Zagorski W, Knudsen ES (2007) RB loss promotes aberrant ploidy by deregulating levels and activity of DNA replication factors. J Biol Chem 282: 23867–23877.
  50. 50. Mayhew CN, Carter SL, Fox SR, Sexton CR, Reed CA, et al. (2007) RB loss abrogates cell cycle control and genome integrity to promote liver tumorigenesis. Gastroenterology 133: 976–984.
  51. 51. Celton-Morizur S, Desdouets C (2010) Polyploidization of liver cells. Adv Exp Med Biol 676: 123–135.
  52. 52. Ganem NJ, Storchova Z, Pellman D (2007) Tetraploidy, aneuploidy and cancer. Curr Opin Genet Dev 17: 157–162.
  53. 53. Murakami H (1993) Transgenic mouse model for synergistic effects of nuclear oncogenes and growth factors in tumorigenesis: interaction of c-myc and transforming growth factor alpha in hepatic oncogenesis. Cancer Res 53: 1719–1723.
  54. 54. Sargent LM (1999) Nonrandom cytogenetic alterations in hepatocellular carcinoma from transgenic mice overexpressing c-Myc and transforming growth factor-[alpha] in the liver. Am J Pathol 154: 1047–1055.
  55. 55. Santoni-Rugiu E, Jensen MR, Thorgeirsson SS (1998) Disruption of the pRb/E2F Pathway and Inhibition of Apoptosis Are Major Oncogenic Events in Liver Constitutively Expressing c-myc and Transforming Growth Factor alpha. Cancer Research 58: 123–134.
  56. 56. Calvisi DF, Factor VM, Ladu S, Conner EA, Thorgeirsson SS (2004) Disruption of beta-catenin pathway or genomic instability define two distinct categories of liver cancer in transgenic mice. Gastroenterology 126: 1374–1386.
  57. 57. Kaposi-Novak P, Libbrecht L, Woo HG, Lee YH, Sears NC, et al. (2009) Central role of c-Myc during malignant conversion in human hepatocarcinogenesis. Cancer Res 69: 2775–2782.
  58. 58. Hoshida Y, Nijman SM, Kobayashi M, Chan JA, Brunet JP, et al. (2009) Integrative transcriptome analysis reveals common molecular subclasses of human hepatocellular carcinoma. Cancer Res 69: 7385–7392.
  59. 59. Mateyak MK, Obaya AJ, Adachi S, Sedivy JM (1997) Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination. Cell Growth Differ 8: 1039–1048.
  60. 60. Kim JW, Mori S, Nevins JR (2010) Myc-induced microRNAs integrate Myc-mediated cell proliferation and cell fate. Cancer Res 70: 4820–4828.
  61. 61. Santoni-Rugiu E, Falck J, Mailand N, Bartek J, Lukas J (2000) Involvement of Myc activity in a G(1)/S-promoting mechanism parallel to the pRb/E2F pathway. Mol Cell Biol 20: 3497–3509.
  62. 62. Sears RC, Nevins JR (2002) Signaling networks that link cell proliferation and cell fate. J Biol Chem 277: 11617–11620.
  63. 63. Chen D, Pacal M, Wenzel P, Knoepfler PS, Leone G, et al. (2009) Division and apoptosis of E2f-deficient retinal progenitors. Nature 462: 925–929.
  64. 64. Macpherson D (2008) Insights from mouse models into human retinoblastoma. Cell Div 3: 9.
  65. 65. Edamoto Y, Hara A, Biernat W, Terracciano L, Cathomas G, et al. (2003) Alterations of RB1, p53 and Wnt pathways in hepatocellular carcinomas associated with hepatitis C, hepatitis B and alcoholic liver cirrhosis. Int J Cancer 106: 334–341.
  66. 66. Knudsen ES, Knudsen KE (2008) Tailoring to RB: tumour suppressor status and therapeutic response. Nat Rev Cancer 8: 714–724.
  67. 67. Laurent-Puig P, Zucman-Rossi J (2006) Genetics of hepatocellular tumors. Oncogene 25: 3778–3786.
  68. 68. Mayer RJ, Fujita J (2006) Gankyrin, the 26 S proteasome, the cell cycle and cancer. Biochem Soc Trans 34: 746–748.
  69. 69. Hsieh YH, Su IJ, Wang HC, Tsai JH, Huang YJ, et al. (2007) Hepatitis B virus pre-S2 mutant surface antigen induces degradation of cyclin-dependent kinase inhibitor p27Kip1 through c-Jun activation domain-binding protein 1. Mol Cancer Res 5: 1063–1072.
  70. 70. Park SG, Chung C, Kang H, Kim JY, Jung G (2006) Up-regulation of cyclin D1 by HBx is mediated by NF-kappaB2/BCL3 complex through kappaB site of cyclin D1 promoter. J Biol Chem 281: 31770–31777.
  71. 71. Wang WH, Hullinger RL, Andrisani OM (2008) Hepatitis B virus X protein via the p38MAPK pathway induces E2F1 release and ATR kinase activation mediating p53 apoptosis. J Biol Chem 283: 25455–25467.
  72. 72. Kim YJ, Jung JK, Lee SY, Jang KL (2009) Hepatitis B virus X protein overcomes stress-induced premature senescence by repressing p16(INK4a) expression via DNA methylation. Cancer Lett.
  73. 73. McGivern DR, Lemon SM (2009) Tumor suppressors, chromosomal instability, and hepatitis C virus-associated liver cancer. Annu Rev Pathol 4: 399–415.
  74. 74. Ho VM, Schaffer BE, Karnezis AN, Park KS, Sage J (2009) The retinoblastoma gene Rb and its family member p130 suppress lung adenocarcinoma induced by oncogenic K-Ras. Oncogene.
  75. 75. Junttila MR, Karnezis AN, Garcia D, Madriles F, Kortlever RM, et al. (2010) Selective activation of p53-mediated tumour suppression in high-grade tumours. Nature 468: 567–571.
  76. 76. Feldser DM, Kostova KK, Winslow MM, Taylor SE, Cashman C, et al. (2010) Stage-specific sensitivity to p53 restoration during lung cancer progression. Nature 468: 572–575.
  77. 77. Manning AL, Longworth MS, Dyson NJ (2010) Loss of pRB causes Centromere Dysfunction and Chromosomal Instability. Genes Dev.
  78. 78. Sage J, Straight AF (2010) RB's original CIN? Genes Dev 24: 1329–1333.
  79. 79. Stadtfeld M, Hochedlinger K (2010) Induced pluripotency: history, mechanisms, and applications. Genes Dev 24: 2239–2263.
  80. 80. van Harn T, Foijer F, van Vugt M, Banerjee R, Yang F, et al. (2010) Loss of Rb proteins causes genomic instability in the absence of mitogenic signaling. Genes Dev.
  81. 81. Cimini D (2008) Merotelic kinetochore orientation, aneuploidy, and cancer. Biochim Biophys Acta 1786: 32–40.
  82. 82. Fujiwara T, Bandi M, Nitta M, Ivanova EV, Bronson RT, et al. (2005) Cytokinesis failure generating tetraploids promotes tumorigenesis in p53-null cells. Nature 437: 1043–1047.
  83. 83. Burkhart DL, Ngai LK, Roake CM, Viatour P, Thangavel C, et al. (2010) Regulation of RB transcription in vivo by RB family members. Mol Cell Biol 30: 1729–1745.