HIV-1 infection has been on the rise in Japan recently, and the main transmission route has changed from blood transmission in the 1980s to homo- and/or hetero-sexual transmission in the 2000s. The lack of early viral samples with clinical information made it difficult to investigate the possible virological changes over time. In this study, we sequenced 142 full-length env genes collected from 16 Japanese subjects infected with HIV-1 in the 1980s and in the 2000s. We examined the diversity change in sequences and potential adaptive evolution of the virus to the host population. We used a codon-based likelihood method under the branch-site and clade models to detect positive selection operating on the virus. The clade model was extended to account for different positive selection pressures in different viral populations. The result showed that the selection pressure was weaker in the 2000s than in the 1980s, indicating that it might have become easier for the HIV to infect a new host and to develop into AIDS now than 20 years ago and that the HIV may be becoming more virulent in the Japanese population. The study provides useful information on the surveillance of HIV infection and highlights the utility of the extended clade models in analysis of virus populations which may be under different selection pressures.
Citation: Yoshida I, Sugiura W, Shibata J, Ren F, Yang Z, Tanaka H (2011) Change of Positive Selection Pressure on HIV-1 Envelope Gene Inferred by Early and Recent Samples. PLoS ONE6(4): e18630. https://doi.org/10.1371/journal.pone.0018630
Editor: David Liberles, University of Wyoming, United States of America
Received: August 28, 2010; Accepted: March 11, 2011; Published: April 19, 2011
Copyright: © 2011 Yoshida et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, and Technology of Japan to F.R. and H.T (19510208). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Whether human immunodeficiency virus type 1 (HIV-1) has reached peak virulence or has started evolving toward attenuation is controversial, with different studies suggesting that HIV-1 virulence has been increasing , , , , , , stable ,  or decreasing , . Arien et al.  proposed a model in which the viral virulence can be either attenuated or increasing depending on the genetic diversity of the host population. In a human population with mixed HLA (Human Leukocyte Antigen) alleles and diverse host polymorphisms, the CTL (cytotoxic T lymphocyte) response of the recipient may recognize a different set of HIV-1 epitopes from the donor, so that new mutations in viral epitopes may be necessary for CTL escape, which may cause a reduced viral fitness and lead to HIV-1 attenuation. In contrast, in a homogenous human population with little HLA and genetic diversity, the virus with acquired escape mutations from the donor may escape the CTL response of the recipient as well, so that the virus may become even more virulent leading to rapid disease progression.
In Japan, the number of HIV-1 infected individuals is increasing in recent years, and the main route of infection has changed from blood transmission in the 1980s to homo- and/or heterosexual transmission in the 2000s. The change of transmission routes may affect the pathogenicity of the virus circulating in the population. However, the lack of early viral samples makes it difficult to study possible changes in viral pathogenicity. In this study, we sequenced the full-length env gene from HIV-1 samples collected in the late 1980s from six Japanese subjects. In order to investigate possible changes in viral diversity and pathogenicity, we also sequenced the env gene from HIV-1 samples collected in the 2000s from 10 Japanese subjects.
The env gene is the fastest-evolving gene in the HIV-1 genome , , , . While new mutations in the env gene may allow the virus to escape from host immune response, they may also disrupt the function of env as the viral envelope. Such conflicting selective pressures shape the evolutionary dynamics of the virus and its population diversity. In this study, we are interested in the extent by which the env gene has been able to diversify at the population and individual levels, and whether the virus has been undergoing adaptive evolution. Since CD4 counts and information on viral load were unavailable for the early samples, we employed computational approaches to infer diversity changes and to detect positive selection acting on the env gene. In particular, we are interested in whether positive selection pressure differs between the early and recent samples and between within-host and between-host evolution , . We are also interested in detecting sites in the env gene targeted by the human immune system.
Sequences from seven early subjects and 10 recent subjects were successfully obtained
Information concerning the transmission routes and sampling times of all subjects are shown in Table 1. Since not all the early samples were preserved in ideal conditions, we sequenced only seven of the 16 early subjects. By using Subtype Reference Alignments , the viral samples from one of seven early subjects were identified as subtype C, while all other early subjects as well as all recent subjects were confirmed to be subtype B. We thus removed the subtype C samples from our analysis. Forty-four sequences for the early group were obtained from PBMC (peripheral blood mononuclear cell) samples as the blood plasma samples could not be amplified by PCR, while 98 sequences for the recent group were obtained from blood plasma samples. Only 2 sequences were collected from each of subjects 3 and 31 in the early group. In total, 142 full-length env gene sequences were obtained from the 16 subjects and used for further analysis. These sequences have been deposited in the DDBJ/EMBL/GenBank databases under accession numbers AB588196-AB588337 (142 entries).
As the codon-based analysis assumes no recombination within the sequence, we run the program RIP 3.0 to detect possible recombinants. The results showed that none of the 142 subtype B sequences was recombinant with other subtypes. For co-receptor usage, WebPSSM program predicted all samples to be CCR5-using viruses.
No significant difference in within-host diversity between the two groups
The phylogenetic tree of 142 env sequences was reconstructed using the Neighbor-joining method in MEGA4  under the K80+G model  (Fig. 1). This tree is used in the codon-based maximum likelihood (ML) analysis. The robustness of our results to the tree topology is examined later, by duplicating the analysis using the ML tree under the GTR+G model (PhyML) . In both the NJ and ML trees, viral sequences from the same subject formed a distinct cluster, with the between-host branches to be much longer than the within-host branches. These results are compatible with a severe bottleneck at each new infection.
The tree was constructed by using the neighbour-joining method (MEGA4). The 16 subjects are indicated as S_subject-number (Table 1). Viral samples from the 1980s are indicated in red while those from the 2000s are in blue. The higher bootstrap values (>70%) for major nodes were shown on the tree.
The viral diversity was calculated by measuring nucleotide diversity (π) implemented in MEGA4. The between-host diversity was inferred to be 0.062 for samples of the 1980s and 0.118 for samples of the 2000s. The recent group had larger between-host diversity than the early group, which may simply reflect the accumulation of new mutations over time. The within-host diversities for the early and recent samples almost remained the same (0.012 vs. 0.011).
Positive selection on the viral env gene has become weaker in the 2000s
We used the branch-site model , ,  to detect positive selection operating on the HIV-1 env gene, with the selective pressure measured by the nonsynonymous/synonymous rate ratio ω . In this model, branches on the phylogeny are partitioned a priori into two categories: the foreground branches which may potentially be under positive selection, and the background branches along which positive selection is assumed to be absent. On the background branches, some sites are strongly conserved with 0<ω0<1 while others are evolving neutrally with ω1 = 1. On the foreground branches some of those sites become under positive selection with ω2≥1. The parameters in the model representing the proportions of site classes and the ω ratios are summarized in Table 2. The branch-site test compares the null model which assumes ω2 = 1 against the alternative model with ω2≥1, with one degree of freedom used. The test allows detection of positive selection affecting the foreground branches even though most codons in the gene are under purifying selection.
We expect the human immune system to exert selective pressure on the virus, but the pressure may differ in the 1980s and in the 2000s or between within-host and between-host evolution. We conducted four analyses in which different branches in the reconstructed tree (Fig. 1) were designated as the foreground branches: (a) 1980s-within, (b) 2000s-within, (c) 1980s-between, and (d) 2000s-between (Fig. S2). The results are summarized in Table 3. The test result was significant with p<1% in all four analyses, indicating that positive selection most likely operated during both within-host evolution and between-host evolution and both in the 1980s and in the 2000s. Estimates of ω2 under the model suggest that the selection pressure was stronger between hosts than within host. Interestingly, the early samples were under stronger selection even though the recent samples showed higher between-host diversity. We note that the use of ω2 estimates in the branch-site model to measure the strength of positive selection may suffer from the strong correlation between estimates of p2 and ω2, as it is difficult to distinguish fewer sites under strong selection from more sites under weak selection. Thus we also calculated another heuristic measure of positive selection pressure: p2ω2, with the expectation that both a higher ω2 and a higher p2 indicate stronger positive selection. Use of this measure instead of ω2 leads to the same conclusions.
The branch-site model has the limitation that it allows only two types of branches (foreground and background) and positive selection is assumed not to occur on the background branches. For the HIV-1 env genes, it is possible that all branches on the tree have some sites under positive selection. The assumption of no positive selection on the background branches may affect the estimation of the strength of positive selection pressure along the foreground branches. Thus we extended the clade model C of Bielawski and Yang  to allow for more than two branch types (see Methods). The original model C allows for two branch types (clades) and assumes three site classes: site class 0 of conserved sites with ω0<1, site class 1 of neutral sites with ω1 = 1, and site class 2 with different selective pressures (ω2 and ω3) in the two clades. We extend this model to allow for more than two branch types, which have different rate ratios ω2 and ω3, … for site class 2 . We specify five branch types in our analysis: 1980s-within, 2000s-within, 1980s-between, and 2000s-between, with all other branches grouped into one branch type. The results are summarized in Table 4. Estimates of the ω ratios for site class 2 for the five branch types (ω2-ω6) indicate positive selection for each branch type, but the selective pressure is weaker in the 2000s than in the 1980s. Indeed, estimates of ω2-ω6 under the clade model are in the same order as estimates of ω2 under the branch-site model when the four branch types were individually designated as the foreground branches (Table 3). The results obtained from the two analyses are thus consistent.
We furthermore used the extended clade model to conduct two likelihood ratio tests to examine whether the positive selection pressure has changed between the 1980s and the 2000s. The null hypothesis for the first test is that the positive selection pressure for within-host evolution is the same in the 1980s and in the 2000s (ω3 = ω4). The log likelihood was calculated either with or without this constraint, producing 2Δℓ = 32.10. The null hypothesis is thus rejected, with p<1%. The null hypothesis for the second test is that the positive selection pressure for between-host evolution is the same in the 1980s and in the 2000s (ω5 = ω6). This is also rejected, with 2Δℓ = 22.06, and p<1% and d.f. = 1.
Positively selected sites were detected in both early and recent samples
Amino acid sites inferred to be under positive selection by the BEB (Bayes Empirical Bayes) approach under the branch-site model at the P = 95% level are listed in Table 3. We compared those sites with antibody binding sites in the HIV Molecular Immunology Database . Some inferred sites are located in indel-rich regions and are difficult to be identified in the reference sequence HXB2. These sites were excluded. All other inferred sites were identified within at least one of epitope regions for antibody, CTL/CD8 and T-Helper/CD4 (Table 5). Furthermore, each of the four branch-site analyses detected three sites under positive selection within one of the epitopes presented by HLA alleles commonly observed in the Japanese population (with frequency >10%) . Importantly, two of the three sites in the V1-V5 regions, 204V and 420K, were detected to be under positive selection along the between-1980s branches, whereas none of them was detected along the between-2000s branches.
Robustness of our results
Yap et al. (; see also ) are concerned that estimation of the ω ratio may be sensitive to model assumptions concerning codon usage. The authors suggested alternative ways of accommodating nucleotide/codon frequencies in models of codon substitution. Those models, like most early ones, are not based on our understanding of the biological process but are instead mathematical constructs aimed at fitting the datasets empirically. Nevertheless the potential sensitivity of our results to model assumptions is a concern. We have examined the nucleotide frequencies in our dataset, and found that they were nearly identical between the recent and early viral samples. Our analysis has been conducted under the F3×4 model of codon usage, with three nucleotide frequency parameters used for each codon position . To examine the robustness of the results to assumptions concerning codon usage, we repeated the analysis under the Fcodon model (CodonFreq = 3 in codeml), which uses the 61 codon frequencies as parameters. All frequency parameters under the F3×4 and Fcodon models are estimated using the observed frequencies in the data. The results obtained under the Fcodon model are listed in Supplementary Tables S1 and S2 for the branch-site and clade models, respectively. They were very similar to those obtained in the corresponding analyses under the F3×4 model, indicating that our results may be robust to assumptions concerning nucleotide/codon frequencies.
Furthermore, we assessed the impact of the tree topology on our analysis. Instead of the NJ tree under K80+G, we also used the ML tree inferred using PhyML under GTR+G. Note that only the tree topology is used in the codon-based analysis, and branch lengths are re-estimated under the codon model. The results for the ML tree are included in Supplementary Tables S3 and S4 for the branch-site and clade models, respectively. These are highly similar to the results obtained using the NJ tree, suggesting that our conclusions may be robust to minor errors in the tree topology.
In this study, we contrast the changes in genetic diversity and adaptive evolution of the HIV-1 env gene between samples collected in the 1980s, the beginning of HIV-1 pandemics, and those collected after 2000, when the virus had spread worldwide and multiple human to human transmissions had taken place.
We sequenced HIV-1 env genes from seven subjects sampled in the 1980s. The samples from six of seven subjects were identified as subtype B. This finding is consistent with the observation that subtype B has been the dominating subtype in Japan since 1980s . Since the early blood plasma included heparin that inhibits PCR, none of the early samples could be amplified from blood plasma and we had to use PBMC instead. We were able to obtain full-length env genes from both early and recent samples in this study, which gave our analysis an advantage over most previous studies on the env gene, which usually used only partial env sequences , , .
No significant change in within-host diversity was found after nearly two decades of evolution. The reconstructed phylogenetic tree of 142 sequences demonstrated distinctly long internal branches and short external branches, suggesting that only a small number of viruses infected the new host cell at each transmission so that these founder viruses usually are quite different among hosts. Moreover, the viruses that successfully infected new host cells are under strong selective pressure from the host immune system, which limited within-host diversification, as indicated by those small clusters on the tree. Therefore, those individual-specific mutations harbored by founder viruses may have a large impact on the within-host evolution and affect the prognosis of HIV infection.
In between-host HIV evolution, the reset of viral fitness by a genetic bottleneck may play an important role, influenced by both viral and host factors. Arien et al.  described two different HIV transmission scenarios for human populations with either diverse or homogeneous genetic backgrounds. We note that Arien et al. 's argument does not yet constitute a quantitative model with precise mathematical predictions. For example, it is unclear what levels of host genetic variation should cause the HIV to become attenuated or more virulent. Nevertheless, a previous study, which examined adaptive HIV-specific immune responses and viral evolution in adult monozygotic twins simultaneously infected with the same virus, provided qualitative support for the model . The study found that 15 out of 17 epitopes targeted by initial CD8 T cell response were identical in each twin, indicating the concordance of adaptive immune responses in the same genetic background.
In our analysis, the between-host selective pressures were inferred to be weaker for the recent samples than for the early samples, in spite of the fact that the recent samples had higher diversity between hosts. For the early group, three out of six subjects were infected by the blood products imported from a foreign country, and the others were most likely infected with the virus from overseas nationals, representing the transmissions between the populations with different genetic backgrounds. In contrast, all the 10 subjects from the recent group represent transmission within native Japanese population. Several studies have reported that the genetic diversity in the Japanese population is very small , , . In a study of gene-based SNP discovery, Haga et al.  found one polymorphism per 807 bp in the Japanese population, much lower than one SNP got every 272 bp for the world average , indicating that the Japanese population appears to be more homogeneous. Accordingly, the new CTL escape mutation upon transmission to a new host in the 2000s will be less necessary than in the 1980s. Since the CTL escape mutations in viral epitopes usually exact a cost to viral fitness , the HIV transmission with fewer escape mutations will have a lower cost in viral fitness. In other words, in the 2000s, the HIV may have a higher viral fitness after the transmission to a new host. The viral fitness is a key factor affecting the viral virulence which measures the capacity of a virus to cause disease . Thus, we speculate that the HIV circulating in the homogeneous Japanese population may have evolved to be more virulent.
The results of Table 5 support this speculation. In spite of more sites being detected under positive selection for between-host evolution for the recent group than for the early group, none of those sites was in the V1–V5 regions, suggesting that some mutations in these epitopes, although needed for a new infection in the 1980s, may have become fixed in the population in the 2000s. In a recent study, Kawashima et al.  demonstrated strong evidence of HIV adaptation to HLA at a population level. The authors pointed out that the process of viral adaptation might alter currently established HLA associations with slow disease progression to AIDS. Although pol and gag genes were analyzed in their study, it appears sensible to expect similar adaptive evolution to occur on the env gene as well. Collection of more clinical data may shed light on this issue.
While the size of our data for the early samples is relatively small, our search in the HIV Sequence Database  did not locate any full-length env genes collected in the 1980s from the Japanese population. Instead, we found 21 partial sequences covering V2, V3, C2 and part of C3 regions of the env gene. These sequences were sampled from two Japanese subjects in 1988: subject 9 (AB002885-AB002894) and subject 20 (AB002922-AB002932) . Subject 9 was infected through HIV contaminated blood products during 1983–1985 and subject 20 was infected through sexual transmission in 1986. Those partial sequences were aligned with the same region of our 142 full-length env genes using ClustalW to form a new dataset of 163 sequences, each of 693 bp. This V2–V3 dataset was analyzed in the same way as the dataset for the full-length env genes. The phylogeny was reconstructed using the NJ method, which was further used to detect positive selection using the branch-site and extended clade models. The reconstructed phylogenetic tree showed that the sequences obtained from each subject most tightly clustered together and were divergent from those of other subjects (Fig. S1). The codon-based analysis produced similar results to those from the full-length dataset. For example, the branch-site model suggested that the positive selection pressure along the 2000s-between branches (ω6 = 3.462) was significantly weaker than for 1980s-between (ω5 = 7.124), with 2Δℓ = 6.24. However, the V2–V3 dataset is less informative due to its smaller size (231 codons vs. 926 codons in the full-length dataset), and did not lead to significant results in some tests (results not shown).
The present study also highlights the utility of the branch-site and extended clade models in analysis of viral samples when the viral gene may be under different positive selection pressures during different time periods or in different viral populations. By using pre-specified branch types, to represent viral evolution within hosts and transmission between hosts, we can estimate ω ratios for different branch types and test potential differences in selective pressure during different periods of viral evolution. Notably, the extended clade model appears to be considerably useful to detect positive selection in the case that multiple viral lineages in the phylogeny may have been evolving under different selective pressures. In our study, the model performed well in both analysis of the full-length gene and the V2–V3 region datasets.
Materials and Methods
All the blood samples were preserved in the National Institute of Infectious Diseases (NIID) in Tokyo. Transmission routes and sampling times were available for all subjects but detailed clinical information was unavailable for the subjects collected in 1980s (Table 1). All subjects were Japanese and did not receive anti-HIV therapy. Both PBMC and blood plasma were available for early samples (continuously stored at −130°C), whereas blood plasma only was available for recent samples. This study was conducted according to the principles expressed in the Declaration of Helsinki. The study was approved by the Institutional Review Board of the NIID. All patients provided written informed consent for the collection of samples and subsequent analysis.
Sequencing and phylogenetic analysis
Proviral DNA was extracted from PBMC using the QIAamp DNA Blood Mini Kit. Viral RNA was extracted from blood plasma using the MagNa Pure Compact Nucleic Acid Isolation Kit I (Roche Applied Science). RNA products were used for cDNA synthesis, using Superscript II RT Kit and random hexamer (Invitrogen).
DNA products were sequenced using the Single-Genome Sequencing (SGS) method (25). For full-length env gene amplification, they were PCR amplified using a set of primers (Env-6116F: 5′ - gcaatagttgtgtggwcyatag -3′ and Env-1M: 5′ - tagcccttccagtccccccttttctttta - 3′) followed by nested amplification (Env-SF1: 5′ - ctaatagaaagagcagaagacagtgg -3′ and Env-PR2: 5′ - gctsccttrtaagtcattggtct -3′). The PCR products sequenced with an ABI PRISM 3730 automated DNA sequencer (Applied Biosystems).
To identify the subtypes of the viral samples sequenced in this study, we used Subtype Reference Alignments . We also tested for recombination using the program RIP 3.0 (Recombinant Identification Program) available at the same Website. Moreover, since the infection time of some subjects was unknown (Table 1), we used WebPSSM  to predict the co-receptor usage, as it has been known that CCR5-using viruses are important during early stages of the HIV infection, whereas CXCR4-using viruses emerge later in the progression to AIDS . The default option, x4r5 matrix, was used for the prediction.
The sequences were aligned using ClustalW. First, the 142 nucleotide sequences were translated into amino acid sequences using MEGA4, which were aligned using ClustalW implemented in MEGA4. Then the aligned amino acid sequences were used to construct the nucleotide alignment. There are 926 codons (2778 bp) in the alignment. We used the software FindModel  to find the appropriate substitution model. Models GTR+G, HKY+G, K80+G and TrN+G were suggested to have better AIC scores and likelihoods. Then we reconstructed the phylogenetic tree using the ML method (PhyML)  under GTR+G and the NJ method (MEGA4)  under K80+G  (the GTR+G model is not available in MEGA4). As the resultant trees were similar, we used the NJ tree for further analysis. However, the ML tree under the GTR+G model was used to duplicate the analyses to confirm the robustness of our results to possible topological errors.
Inferring the genetic diversity of the envelope gene
To investigate the diversity change, we inferred within- and between-host mean diversities for the 1980s and the 2000s, respectively, using the nucleotide diversity, π, implemented in MEGA4 under the K80+G model again. As the sequences are highly similar, the choice of the substitution model makes little difference to the distance calculation. For Setup Data, the viral sequences obtained from the same subject were grouped as one subpopulation. Then, the within-host diversity was calculated by Mean Diversity within Subpopulations, whereas the between-host diversity was calculated by Mean Interpopulational Diversity.
Detecting temporal changes of positive selection pressure using codon models
We used codon models of coding sequence evolution to detect positive selection operating on the HIV-1 env gene. In particular, we are interested in differences in positive selection pressure on the virus between the 1980s and the 2000s, and between within-host evolution and between-host evolution. We use the branch-site model ,  to detect such temporal changes in positive selection pressure, as implemented in the codeml program in the paml package . The likelihood ratio test compares the branch-site model A with a null model that fixes ω2 = 1, with one degree of freedom used. This phylogeny-based analysis assumes that there is no recombination with the sequence so that all sites in the sequence are related by the same phylogenetic tree and that the substitution process has been stationary. The codon frequencies are described using the F3×4 model, which uses the nucleotide frequencies at the three codon positions to describe the codon usage, with 9 free parameters used. The frequency parameters are estimated using the frequencies observed in the data. The branch-site model makes rather stringent assumptions about the selective pressures acting on the foreground and background branches. Simulations, however, suggest that the test is quite robust if the real selective scheme is far more complex .
The assumption, made in the branch-site model, of no positive selection on the background branches and of only two branch types may be too restrictive for the HIV-1 env genes. Thus we extend the clade model C of Bielawski and Yang  to allow for more than two branch types. Clade model C was developed to detect divergent evolution in two clades (two branch types): with site class 0 to include conserved sites with ω0<1, class 1 to be neutral sites with ω1 = 1 and class 2 represent sites undergoing divergent evolution with different selective pressures (ω2 and ω3) in the two clades. We extend this model to allow for more than two branch types, which have different rate ratios ω2 and ω3, …, for site class 2, where 0<ω2, ω3, …<∞. The model thus accounts for conserved sites, nearly neutral sites, as well as positive-selection sites that are under different levels of selection among the branch types. We have also extended the Bayes empirical Bayes (BEB) method to calculate the posterior probabilities for the site classes when there are more than two branch types . The extended clade model has been implemented in the codeml program in the paml package . For the analysis of our data, we used five branch types: 1980s-within, 2000s-within, 1980s-between, and 2000s-between, with all other branches grouped into one branch type.
Unrooted phylogenetic tree of 163 V2–V3 region sequences from 16 Japanese subjects. The tree was constructed by using the neighbour-joining method (MEGA4). The 2 subjects found in the database are indicated as “S_88JP9” and “S_88JP20” respectively. The red represents viral samples from the 1980s group while the blue represents those from the 2000s group.
The foreground branches in the different analyses under the branch-site model. The tree topology is the same as in Fig. 1, but different foreground branches are assumed in four analysis: (a) within-1980s (red), (b) within-2000s (blue), (c) between-1980s (red), and (d) between-2000s (blue).
Log-likelihood values and parameter estimates under the branch-site models using the Fcodon model (CodonFreq = 3).
Log-likelihood values and parameter estimates under the clade model using the Fcodon model (CodonFreq = 3).
Log-likelihood values and parameter estimates under the branch-site models using GTR+G model (CodonFreq = 2).
We thank Von Bing Yap and three anonymous reviewers for constructive criticism and suggestions in the preparation of this manuscript.
Conceived and designed the experiments: IY WS FR ZY HT. Performed the experiments: IY JS WS. Analyzed the data: IY FR ZY HT. Contributed reagents/materials/analysis tools: IY JS WS ZY FR HT. Wrote the paper: FR ZY IY WS JS.
- 1. Crum-Cianflone N, Eberly L, Zhang Y, Ganesan A, Weintrob A, et al. (2009) Is HIV becoming more virulent? Initial CD4 cell counts among HIV seroconverters during the course of the HIV epidemic: 1985–2007. Clin Infect Dis 48: 1285–1292.N. Crum-CianfloneL. EberlyY. ZhangA. GanesanA. Weintrob2009Is HIV becoming more virulent? Initial CD4 cell counts among HIV seroconverters during the course of the HIV epidemic: 1985–2007.Clin Infect Dis4812851292
- 2. Dorrucci M, Phillips AN, Longo B, Rezza G (2005) Changes over time in post-seroconversion CD4 cell counts in the Italian HIV-Seroconversion Study: 1985–2002. Aids 19: 331–335.M. DorrucciAN PhillipsB. LongoG. Rezza2005Changes over time in post-seroconversion CD4 cell counts in the Italian HIV-Seroconversion Study: 1985–2002.Aids19331335
- 3. Dorrucci M, Rezza G, Porter K, Phillips A (2007) Temporal trends in postseroconversion CD4 cell count and HIV load: the Concerted Action on Seroconversion to AIDS and Death in Europe Collaboration, 1985–2002. J Infect Dis 195: 525–534.M. DorrucciG. RezzaK. PorterA. Phillips2007Temporal trends in postseroconversion CD4 cell count and HIV load: the Concerted Action on Seroconversion to AIDS and Death in Europe Collaboration, 1985–2002.J Infect Dis195525534
- 4. Gali Y, Berkhout B, Vanham G, Bakker M, Back NK, et al. (2007) Survey of the temporal changes in HIV-1 replicative fitness in the Amsterdam Cohort. Virology 364: 140–146.Y. GaliB. BerkhoutG. VanhamM. BakkerNK Back2007Survey of the temporal changes in HIV-1 replicative fitness in the Amsterdam Cohort.Virology364140146
- 5. Muller V, Maggiolo F, Suter F, Ladisa N, De Luca A, et al. (2009) Increasing clinical virulence in two decades of the Italian HIV epidemic. PLoS Pathog 5: e1000454.V. MullerF. MaggioloF. SuterN. LadisaA. De Luca2009Increasing clinical virulence in two decades of the Italian HIV epidemic.PLoS Pathog5e1000454
- 6. Vanhems P, Lambert J, Guerra M, Hirschel B, Allard R (1999) Association between the rate of CD4+ T cell decrease and the year of human immunodeficiency virus (HIV) type 1 seroconversion among persons enrolled in the Swiss HIV cohort study. J Infect Dis 180: 1803–1808.P. VanhemsJ. LambertM. GuerraB. HirschelR. Allard1999Association between the rate of CD4+ T cell decrease and the year of human immunodeficiency virus (HIV) type 1 seroconversion among persons enrolled in the Swiss HIV cohort study.J Infect Dis18018031808
- 7. Herbeck JT, Gottlieb GS, Li X, Hu Z, Detels R, et al. (2008) Lack of evidence for changing virulence of HIV-1 in North America. PLoS One 3: e1525.JT HerbeckGS GottliebX. LiZ. HuR. Detels2008Lack of evidence for changing virulence of HIV-1 in North America.PLoS One3e1525
- 8. Muller V, Ledergerber B, Perrin L, Klimkait T, Furrer H, et al. (2006) Stable virulence levels in the HIV epidemic of Switzerland over two decades. Aids 20: 889–894.V. MullerB. LedergerberL. PerrinT. KlimkaitH. Furrer2006Stable virulence levels in the HIV epidemic of Switzerland over two decades.Aids20889894
- 9. Arien KK, Troyer RM, Gali Y, Colebunders RL, Arts EJ, et al. (2005) Replicative fitness of historical and recent HIV-1 isolates suggests HIV-1 attenuation over time. Aids 19: 1555–1564.KK ArienRM TroyerY. GaliRL ColebundersEJ Arts2005Replicative fitness of historical and recent HIV-1 isolates suggests HIV-1 attenuation over time.Aids1915551564
- 10. Keet IP, Veugelers PJ, Koot M, de Weerd MH, Roos MT, et al. (1996) Temporal trends of the natural history of HIV-1 infection following seroconversion between 1984 and 1993. Aids 10: 1601–1602.IP KeetPJ VeugelersM. KootMH de WeerdMT Roos1996Temporal trends of the natural history of HIV-1 infection following seroconversion between 1984 and 1993.Aids1016011602
- 11. Arien KK, Vanham G, Arts EJ (2007) Is HIV-1 evolving to a less virulent form in humans? Nat Rev Microbiol 5: 141–151.KK ArienG. VanhamEJ Arts2007Is HIV-1 evolving to a less virulent form in humans?Nat Rev Microbiol5141151
- 12. Holmes EC, Zhang LQ, Simmonds P, Ludlam CA, Brown AJ (1992) Convergent and divergent sequence evolution in the surface envelope glycoprotein of human immunodeficiency virus type 1 within a single infected patient. Proc Natl Acad Sci U S A 89: 4835–4839.EC HolmesLQ ZhangP. SimmondsCA LudlamAJ Brown1992Convergent and divergent sequence evolution in the surface envelope glycoprotein of human immunodeficiency virus type 1 within a single infected patient.Proc Natl Acad Sci U S A8948354839
- 13. Leitner T, Kumar S, Albert J (1997) Tempo and mode of nucleotide substitutions in gag and env gene fragments in human immunodeficiency virus type 1 populations with a known transmission history. J Virol 71: 4761–4770.T. LeitnerS. KumarJ. Albert1997Tempo and mode of nucleotide substitutions in gag and env gene fragments in human immunodeficiency virus type 1 populations with a known transmission history.J Virol7147614770
- 14. Korber B, Muldoon M, Theiler J, Gao F, Gupta R, et al. (2000) Timing the ancestor of the HIV-1 pandemic strains. Science 288: 1789–1796.B. KorberM. MuldoonJ. TheilerF. GaoR. Gupta2000Timing the ancestor of the HIV-1 pandemic strains.Science28817891796
- 15. Ross HA, Rodrigo AG (2002) Immune-mediated positive selection drives human immunodeficiency virus type 1 molecular variation and predicts disease duration. J Virol 76: 11715–11720.HA RossAG Rodrigo2002Immune-mediated positive selection drives human immunodeficiency virus type 1 molecular variation and predicts disease duration.J Virol761171511720
- 16. Rambaut A, Posada D, Crandall KA, Holmes EC (2004) The causes and consequences of HIV evolution. Nat Rev Genet 5: 52–61.A. RambautD. PosadaKA CrandallEC Holmes2004The causes and consequences of HIV evolution.Nat Rev Genet55261
- 17. Nickle DC, Heath L, Jensen MA, Gilbert PB, Mullins JI, et al. (2007) HIV-specific probabilistic models of protein evolution. PLoS ONE 2: e503.DC NickleL. HeathMA JensenPB GilbertJI Mullins2007HIV-specific probabilistic models of protein evolution.PLoS ONE2e503
- 18. Leitner TBK, Daniels Marcus , Calef Charles, Foley Brian (2005) HIV-1 Subtype and Circulating Recombinant Form (CRF) Reference Sequences, 2005. TBK LeitnerMarcus DanielsCharles CalefBrian Foley2005HIV-1 Subtype and Circulating Recombinant Form (CRF) Reference Sequences, 2005.Los Alamos National Laboratory, Los Alamos, NM. Los Alamos National Laboratory, Los Alamos, NM.
- 19. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596–1599.K. TamuraJ. DudleyM. NeiS. Kumar2007MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.Mol Biol Evol2415961599
- 20. Kimura M (1980) A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120.M. Kimura1980A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences.J Mol Evol16111120
- 21. Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52: 696–704.S. GuindonO. Gascuel2003A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood.Syst Biol52696704
- 22. Yang Z, Nielsen R (2002) Codon-substitution models for detecting molecular adaptation at individual sites along specific lineages. Mol Biol Evol 19: 908–917.Z. YangR. Nielsen2002Codon-substitution models for detecting molecular adaptation at individual sites along specific lineages.Mol Biol Evol19908917
- 23. Zhang J, Nielsen R, Yang Z (2005) Evaluation of an improved branch-site likelihood method for detecting positive selection at the molecular level. Mol Biol Evol 22: 2472–2479.J. ZhangR. NielsenZ. Yang2005Evaluation of an improved branch-site likelihood method for detecting positive selection at the molecular level.Mol Biol Evol2224722479
- 24. Yang Z, Wong WS, Nielsen R (2005) Bayes empirical bayes inference of amino acid sites under positive selection. Mol Biol Evol 22: 1107–1118.Z. YangWS WongR. Nielsen2005Bayes empirical bayes inference of amino acid sites under positive selection.Mol Biol Evol2211071118
- 25. Yang Z (2007) PAML 4: phylogenetic analysis by maximum likelihood. Mol Biol Evol 24: 1586–1591.Z. Yang2007PAML 4: phylogenetic analysis by maximum likelihood.Mol Biol Evol2415861591
- 26. Bielawski JP, Yang Z (2004) A maximum likelihood method for detecting functional divergence at individual codon sites, with application to gene family evolution. J Mol Evol 59: 121–132.JP BielawskiZ. Yang2004A maximum likelihood method for detecting functional divergence at individual codon sites, with application to gene family evolution.J Mol Evol59121132
- 27. Bielawski JP, Yang Z (2003) Maximum likelihood methods for detecting adaptive evolution after gene duplication. J Struct Funct Genomics 3: 201–212.JP BielawskiZ. Yang2003Maximum likelihood methods for detecting adaptive evolution after gene duplication.J Struct Funct Genomics3201212
- 28. HIV Molecular Immunology Database website. HIV Molecular Immunology Database website.Available: http://www.hiv.lanl.gov/content/immunology/. Accessed: 2010 March 3. Available: http://www.hiv.lanl.gov/content/immunology/. Accessed: 2010 March 3.
- 29. Tokunaga K, Ishikawa Y, Ogawa A, Wang H, Mitsunaga S, et al. (1997) Sequence-based association analysis of HLA class I and II alleles in Japanese supports conservation of common haplotypes. Immunogenetics 46: 199–205.K. TokunagaY. IshikawaA. OgawaH. WangS. Mitsunaga1997Sequence-based association analysis of HLA class I and II alleles in Japanese supports conservation of common haplotypes.Immunogenetics46199205
- 30. Yap VB, Lindsay H, Easteal S, Huttley G (2010) Estimates of the effect of natural selection on protein-coding content. Mol Biol Evol 27: 726–734.VB YapH. LindsayS. EastealG. Huttley2010Estimates of the effect of natural selection on protein-coding content.Mol Biol Evol27726734
- 31. Goldman N, Yang Z (1994) A codon-based model of nucleotide substitution for protein-coding DNA sequences. Mol Biol Evol 11: 725–736.N. GoldmanZ. Yang1994A codon-based model of nucleotide substitution for protein-coding DNA sequences.Mol Biol Evol11725736
- 32. Goudsmit (1998) Viral Sex: The Nature of AIDS. OUP USA; New Ed edition. Goudsmit1998Viral Sex: The Nature of AIDSOUP USA; New Ed edition
- 33. Yamaguchi Y, Gojobori T (1997) Evolutionary mechanisms and population dynamics of the third variable envelope region of HIV within single hosts. Proc Natl Acad Sci USA 94: 1264–1269.Y. YamaguchiT. Gojobori1997Evolutionary mechanisms and population dynamics of the third variable envelope region of HIV within single hosts.Proc Natl Acad Sci USA9412641269
- 34. Yamaguchi-Kabata Y, Gojobori T (2000) Reevaluation of amino acid variability of the human immunodeficiency virus type 1 gp120 envelope glycoprotein and prediction of new discontinuous epitopes. J Virol 74: 4335–4350.Y. Yamaguchi-KabataT. Gojobori2000Reevaluation of amino acid variability of the human immunodeficiency virus type 1 gp120 envelope glycoprotein and prediction of new discontinuous epitopes.J Virol7443354350
- 35. Kupfer B, Sing T, Schuffler P, Hall R, Kurz R, et al. (2007) Fifteen years of env C2V3C3 evolution in six individuals infected clonally with human immunodeficiency virus type 1. J Med Virol 79: 1629–1639.B. KupferT. SingP. SchufflerR. HallR. Kurz2007Fifteen years of env C2V3C3 evolution in six individuals infected clonally with human immunodeficiency virus type 1.J Med Virol7916291639
- 36. Draenert R, Allen TM, Liu Y, Wrin T, Chappey C, et al. (2006) Constraints on HIV-1 evolution and immunodominance revealed in monozygotic adult twins infected with the same virus. J Exp Med 203: 529–539.R. DraenertTM AllenY. LiuT. WrinC. Chappey2006Constraints on HIV-1 evolution and immunodominance revealed in monozygotic adult twins infected with the same virus.J Exp Med203529539
- 37. Ohnishi Y, Tanaka T, Yamada R, Suematsu K, Minami M, et al. (2000) Identification of 187 single nucleotide polymorphisms (SNPs) among 41 candidate genes for ischemic heart disease in the Japanese population. Hum Genet 106: 288–292.Y. OhnishiT. TanakaR. YamadaK. SuematsuM. Minami2000Identification of 187 single nucleotide polymorphisms (SNPs) among 41 candidate genes for ischemic heart disease in the Japanese population.Hum Genet106288292
- 38. Yamada R, Tanaka T, Ohnishi Y, Suematsu K, Minami M, et al. (2000) Identification of 142 single nucleotide polymorphisms in 41 candidate genes for rheumatoid arthritis in the Japanese population. Hum Genet 106: 293–297.R. YamadaT. TanakaY. OhnishiK. SuematsuM. Minami2000Identification of 142 single nucleotide polymorphisms in 41 candidate genes for rheumatoid arthritis in the Japanese population.Hum Genet106293297
- 39. Haga H, Yamada R, Ohnishi Y, Nakamura Y, Tanaka T (2002) Gene-based SNP discovery as part of the Japanese Millennium Genome Project: identification of 190,562 genetic variations in the human genome. Single-nucleotide polymorphism. J Hum Genet 47: 605–610.H. HagaR. YamadaY. OhnishiY. NakamuraT. Tanaka2002Gene-based SNP discovery as part of the Japanese Millennium Genome Project: identification of 190,562 genetic variations in the human genome. Single-nucleotide polymorphism.J Hum Genet47605610
- 40. Kruglyak L, Nickerson DA (2001) Variation is the spice of life. Nat Genet 27: 234–236.L. KruglyakDA Nickerson2001Variation is the spice of life.Nat Genet27234236
- 41. Friedrich TC, Dodds EJ, Yant LJ, Vojnov L, Rudersdorf R, et al. (2004) Reversion of CTL escape-variant immunodeficiency viruses in vivo. Nat Med 10: 275–281.TC FriedrichEJ DoddsLJ YantL. VojnovR. Rudersdorf2004Reversion of CTL escape-variant immunodeficiency viruses in vivo.Nat Med10275281
- 42. Knipe D, Howley PM (2006) D. KnipePM Howley2006Fields Virology: Lippincott Williams & Wilkins; Fifth Edition, Volume one, page 331–333. Fields Virology: Lippincott Williams & Wilkins; Fifth Edition, Volume one, page 331–333.
- 43. Kawashima Y, Pfafferott K, Frater J, Matthews P, Payne R, et al. (2009) Adaptation of HIV-1 to human leukocyte antigen class I. Nature 458(7238): 641–645.Y. KawashimaK. PfafferottJ. FraterP. MatthewsR. Payne2009Adaptation of HIV-1 to human leukocyte antigen class I.Nature4587238641645
- 44. HIV Sequence Database website. HIV Sequence Database website.Available: http://www.hiv.lanl.gov/. Accessed 2010 Jul 5. Available: http://www.hiv.lanl.gov/. Accessed 2010 Jul 5.
- 45. Shioda T, Oka S, Xin X, Liu H, Harukuni R, et al. (1997) In vivo sequence variability of human immunodeficiency virus type 1 envelope gp120: association of V2 extension with slow disease progression. J Virol 71: 4871–4881.T. ShiodaS. OkaX. XinH. LiuR. Harukuni1997In vivo sequence variability of human immunodeficiency virus type 1 envelope gp120: association of V2 extension with slow disease progression.J Virol7148714881
- 46. Jensen MA, Li FS, van 't Wout AB, Nickle DC, Shriner D, et al. (2003) Improved coreceptor usage prediction and genotypic monitoring of R5-to-X4 transition by motif analysis of human immunodeficiency virus type 1 env V3 loop sequences. J Virol 77: 13376–13388.MA JensenFS LiAB van 't WoutDC NickleD. Shriner2003Improved coreceptor usage prediction and genotypic monitoring of R5-to-X4 transition by motif analysis of human immunodeficiency virus type 1 env V3 loop sequences.J Virol771337613388
- 47. Bleul CC, Wu L, Hoxie JA, Springer TA, Mackay CR (1997) The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes. Proc Natl Acad Sci U S A 94: 1925–1930.CC BleulL. WuJA HoxieTA SpringerCR Mackay1997The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.Proc Natl Acad Sci U S A9419251930
- 48. FindModel website. FindModel website.Available: http://www.hiv.lanl.gov/content/sequence/findmodel/findmodel.html. Accessed: 2010 Sep 18. Available: http://www.hiv.lanl.gov/content/sequence/findmodel/findmodel.html. Accessed: 2010 Sep 18.
- 49. Yang Z, Rannala B (2005) Branch-length prior influences Bayesian posterior probability of phylogeny. Syst Biol 54: 455–470.Z. YangB. Rannala2005Branch-length prior influences Bayesian posterior probability of phylogeny.Syst Biol54455470