The seagrass limpet Tectura paleacea (Gastropoda; Patellogastropoda) belongs to a seagrass obligate lineage that has shifted from the Caribbean in the late Miocene, across the Isthmus of Panama prior to the closing of the Panamanian seaway, and then northward to its modern Baja California – Oregon distribution. To address whether larval entrainment by seagrass beds contributes to population structuring, populations were sampled at six California/Oregon localities approximately 2 degrees latitude apart during two post-settlement periods in July 2002 and June 2003. Partial cytochrome oxidase b (Cytb) sequences were obtained from 20 individuals (10 per year) from each population in order to determine the levels of population subdivision/connectivity. From the 120 individuals sequenced, there were eighty-one unique haplotypes, with the greatest haplotype diversity occurring in southern populations. The only significant genetic break detected was consistent with a peri-Point Conception (PPC) biogeographic boundary while populations north and south of Point Conception were each panmictic. The data further indicate that populations found south of the PPC biogeographic boundary originated from northern populations. This pattern of population structure suggests that seagrass patches are not entraining the larvae of T. paleacea by altering flow regimes within their environment; a process hypothesized to produce extensive genetic subdivision on fine geographic scales. In contrast to the haplotype data, morphological patterns vary significantly over very fine geographic scales that are inconsistent with the observed patterns of genetic population structure, indicating that morphological variation in T. paleacea might be attributed to differential ecophenotypic expression in response to local habitat variability throughout its distribution. These results suggest that highly localized conservation efforts may not be as effective as large-scale conservation efforts in near shore marine environments.
Citation: Begovic E, Lindberg DR (2011) Genetic Population Structure of Tectura paleacea: Implications for the Mechanisms Regulating Population Structure in Patchy Coastal Habitats. PLoS ONE6(4): e18408. https://doi.org/10.1371/journal.pone.0018408
Editor: Peter Roopnarine, California Academy of Sciences, United States of America
Received: October 13, 2010; Accepted: March 7, 2011; Published: April 7, 2011
Copyright: © 2011 Begovic, Lindberg. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: Funding for this research was provided by grants in part by the Lerner-Gray Fund, Sigma-Xi, the University of California Museum of Paleontology, and the Department of Integrative Biology at UC Berkeley. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The effects of larval dispersal on marine invertebrate ecology and evolution has long been recognized , , but its implications for evolutionary events such as speciation, extinction, and population structuring have only recently been verified and experimentally studied using molecular data and phylogenetic studies , , . At multiple scales the patterns are clear and often surprising. For example, multiple marine taxa show strong molecular differentiation between populations in the Indian Ocean and Western Pacific, while little variation is present in the expansive Western Pacific . Within the significantly smaller Southern California Bight, Hamm and Burton , using allozymes from Haliotis (abalone), found that genetic distance was independent of geographic distance over a 300 km sampling range, and suggested that limited larval dispersal is involved in generating genetic differentiation. Along the entire California coast, Moberg & Burton  have shown that sea urchins (Strongylocentrotus) also have very heterogeneous genetic compositions on both large and small geographic scales suggesting that, “the larval pool is not well mixed geographically (even on spatial scales <20 km), despite long planktonic larval duration.”
From these and other studies, a picture of the role of larval dispersal in contributing to the genetic makeup of populations is emerging, and it is one that often significantly differs , , ,  from early expectations. This can be partly attributed to the fact that our understanding of the effects of larval dispersal remains incomplete due to (1) limited taxon sampling that has not included less abundant species with patchy distributions, and (2) the lack of attention given to the effects of ecological factors (e.g., habitat specificity and availability, predator-prey interactions, resource availability, etc.) on geographical distributions of genetic diversity.
This study examines the population structure of Tectura paleacea (Gastropoda; Patellogastropoda), a stenotopic species found only on its obligate seagrass host Phyllospadix , . The distribution of T. paleacea is patchy on local and regional scales throughout the Northeastern Pacific (Vancouver, Canada to Baja California, Mexico ). This species is a broadcast spawner with lecithotrophic larvae that can spend anywhere from a few hours to a several days in the plankton , , , . While some studies have shown that taxa with planktonic larvae tend to have low geographic population structure , , other studies find that long planktonic larval duration does not result in low geographic population structuring , . These studies have tried to address the role of hydrogeography and fine scale physical oceanography in larval dispersal success, but conflicting results remain. Other studies have begun to examine the contribution of different ecological roles in population structuring. Ayre et al.  show that phylogeographic breaks within populations of marine organisms correspond to the degree to which the organism can utilize sheltered habitat, while Dawson  demonstrates a correlation between the distribution of edge-effect species and phylogeographic structure. Kelly & Eernisse  hypothesize that variability in near-shore sea surface temperature may be responsible for observed differences in the population structure of species with similar larval dispersal capabilities. These studies show a decoupling between larval dispersal strategies and phylogeographic patterns, but the contributions of specific local biotic factors to the differential success of larval dispersal remain untested.
The primary objective of this study was to measure the relative contribution of habitat specificity on larval dispersal and in turn biogeographic population structure within T. paleacea populations found along the Oregon-California coast. Specifically, we examine the role of seagrass in localized larval retention as an obstacle to gene flow. In doing so we determined the geographical distribution of mitochondrial lineages within T. paleacea and estimated genetic diversity. Furthermore, to evaluate the historical role of dispersal in structuring T. paleacea populations we compare modern-day morphological and mitochondrial geographic patterns.
Materials and Methods
Six localities with extensive Phyllospadix seagrass habitat were identified along the California-Oregon coast (see Figure 1). Each locality was approximately 2 degrees latitude apart and had T. paleacea populations present. The northern sampling boundary, determined by the northern range limit of T. paleacea, was Cape Arago, Oregon . The southern sampling boundary was at Bird Rock in La Jolla, Caifornia, which is approximately 45 km north of the California-Mexico border.
Locality abbreviations are as follows: SR - Simpson Reef, Cape Arago, OR; TH - Trinidad Head, Trinidad, CA; HC - Horseshoe Cove, Bodega, CA; MB - Monterey Bay, CA; CR - Coal Oil Point Reserve, Santa Barbara, CA; BR - Bird Rock, La Jolla, CA. Species distributions for T. paleacea, T. depicta, and Phyllospadix are indicated, as is the peri-Point Conception (PPC) region. The presence of T. depicta in MB appears to be a transient population that is introduced during El Niños (Zimmerman et al. 2001). The inset picture shows T. paleacea on a blade of Phyllospadix (scale bar is included).
Populations of T. paleacea were sampled from each locality at extreme low tides immediately after summer recruitment  during July 2002 and June 2003. Each sampling period entailed the identification of individual seagrass patches (defined as a continuous Phyllospadix rhizome covered substrate, separated by no less than one meter) within each locality. These patches were subsequently mapped using permanent markers embedded into surrounding boulders and triangulation techniques. All size classes of T. paleacea and their associated Phyllospadix blades were collected during timed searches from each Phyllospadix patch. Specimens of T. paleacea and blades of Phyllospadix were stored in 95% EtOH. Relative abundance was estimated in terms of T. paleacea individuals collected per person per unit search time. One-way ANOVA was used to test whether relative abundances for each locality were significantly different (ANOVA calculated using the statistical software package SPSS v11 [SPSS Inc., Chicago IL]).
A total of 763 individuals were collected and deposited in the University of California Museum of Paleontology (see Table S1 for accession numbers). For all subsequent analyses only adults were used (adults measure ≥3.5 mm in length ). Morphological measurements were taken from a sub-sample of 295 individuals and of these, 120 were used for sequencing. With permission, we also included sequence data from two individuals of Tectura depicta collected at San Diego, CA by Kristina D. Louie (unpublished).
Small pieces of foot tissue were excised from a total of 120 T. paleacea adult individuals (10 individuals were sampled from each locality during the 2002 and again during the 2003 sampling period). Total genomic DNA was isolated using the “Animal Tissues” protocol included with the DNEasy Tissue Kit from QUIAGEN, Inc. Valencia CA. A 450 nucleotide fragment of cytochrome oxidase b mtDNA (Cytb) was amplified and sequenced using the following primers: cobF 5′ - GGW TAY GTW YTW CCW TGR GGW CAR AT - 3′; cobR 5′ - GCR TAW GCR AAW ARR AAR TAY CAY TCW GG - 3′ .
No length variation was observed between sequences allowing for manual alignment in Se-Al . Cytb amino acid sequences were used to verify nucleotide sequences and alignment. All sequence types have been deposited in GenBank (see Table S1 for list of specimens and accessions numbers). Two additional Cytb sequence types from T. depicta (the sister taxon of T. paleacea ) were included with permission from Kristina D. Louie (unpublished) for outgroup comparisons and network rooting.
The maximum likelihood model that best fits the combined Cytb data was calculated using a hierarchical Akaike information criterion as implemented in modeltest v3.06 . This test was chosen in place of the likelihood ratio test because it imposes a penalty for model complexity resulting in models with better predictive accuracy . Haplotype relationships were reconstructed with the neighbor-joining (NJ) algorithm and Maximum Likelihood (ML) implementing the transversion model (TVM) in addition to the proportion of invariable sites (I) and gamma distribution (G) models describing variation among sites (TVM+I+G) model of evolution using PAUP* version 4.0b10 . For ML analyses gaps were treated as missing data and the heuristic search algorithm was implemented for ten random addition sequence replicates. Support for haplotype relationships was calculated using fast bootstrap analysis (4168 replicates) , .
Population genetics data analysis
Patterns of haplotype diversity were examined on regional scales by grouping individuals according to phylogenetic affinities. To further test if this grouping accurately reflects population connectivity, each population was serially allocated to unique blocks (i.e., all possible population groupings were examined) and AMOVA analyses , as implemented in arlequin v2.0 , were done on all partitions. Total molecular variance (σ2) was calculated as the sum of the covariance components FST (overall population variation), FSC (within group variation), and FCT (between group variation). Significance tests were based on 16,000 permutations.
Further population genetic data analyses were also carried out using arlequin v2.0. Haplotype and nucleotide diversity was estimated as a means to identify recent changes in effective population sizes. Tajima's  measure of nucleotide diversity (θπ) estimated from the mean number of pairwise nucleotide differences (π) was selected due to its incorporation of allele frequencies, making this statistic more biologically meaningful as compared to others [i.e., segregating sites (θS)] . Furthermore, the observed number of differences between haplotype pairs (i.e., mismatch distribution) was calculated in order to determine if populations are at demographic equilibrium. For example, while a bimodal mismatch distribution is indicative of demographic equilibrium, a unimodal distribution indicates that the population has most likely passed through a recent population expansion , .
Length, width, and height measurements were taken from 25 T. paleacea individual collected from each locality during 2002 and again during 2003. A total of 50 individuals were measured from each locality except in the case of Cape Arago where only 20 individuals were collected in 2003 resulting in a total of 45 individuals being measured from this locality. In total, 295 individuals were measured for morphological analyses. Phyllospadix blade width was measured at the exact point from which each T. paleacea individual was removed from the blade. The relationship of these variables was examined across all localities using ANOVA and correlation statistics. Analyses were done with the statistical software package SPSS v11 (SPSS Inc., Chicago IL).
The relationship between locality and morphology was examined by comparing length:height ratios (LHR) of individuals from each locality. This measure has been established as an appropriate indicator of ecophenotypic variation in marine plant limpets previously . Width was not considered in these comparisons due to the fact that individuals of T. paleacea spend their entire lives on Phyllospadix blades and stems. In turn, the width of these plant structures constrains T. paleacea shell width soon after individuals reach adult size therefore shell width is a reflection of ecophenotypic variation in the plant host and not in the associated limpet. The maximum width of 1.5–2.0 mm is reached when individuals are approximately 3.5 mm long . In order to reduce the bias (i.e., inflating population differentiation) associated with the differential presence of juveniles at each locality (e.g., there were no juveniles found in SR and TH populations while juveniles were collected in abundance from more southern populations), all specimens collected that were smaller than 3.5 mm in length were removed from the morphological analysis.
The contribution of blade and stem constraints to the observed variation in LHR was tested using a series of three comparisons. (1) Shell width of T. paleacea was examined relative to each associated Phyllospadix blade (or stem) width across all localities. (2) ANOVA of Phyllospadix blade width was compared across all localities. In order to test if Phyllospadix blade width at any given locality was significantly affecting shell LHR, (3) the degree of ovalness, measured as width:length ratios (WLR), was compared across all localities.
Of the 120 individuals sequenced, there were eighty-one unique haplotypes present. Twenty individuals were examined from each population resulting in the following haplotype distributions across the six sampled populations: 8 unique haplotypes from Simpson Reef, Cape Arago, OR (SR); 11 from Trinidad Head, Trinidad, CA (TH); 12 form Horseshoe Cove, Bodega, CA (HC); 13 from Monterey Bay, Monterey, CA (MB); 19 from Coal Oil Point Reserve, Santa Barbara, CA (CR); and 18 from Bird Rock, La Jolla, CA (BR). The haplotype relationships determined using a neighbor-joining algorithm are shown in Figure 2 with bootstrap values indicated above each branch of interest. The tree topology resulting from ML analysis is shown in Supplemental Figure S1 with bootstrap values indicated above each branch.
Shows the relationship of 81 haplotypes and two outgroups haplotypes (T. depicta H1 & T. depicta H2). Corresponding haplotype localities are mapped, as is the average shell profile associated with each locality. Arrows indicate the ancestral node of southern clades nested within northern populations.
Population genetics and demography
The relative abundance of T. paleacea populations changed significantly across the sampled range. When plotted against localities by latitude, abundance data appear to have a normal distribution with the highest abundances at the center of the range of T. paleacea (Figure 3).
The hatched line indicates the expected trend for population abundance in Baja, Mexico, the southern most limit of T. paleacea distribution.
AMOVA analyses identified a strong geographic subdivision between the four populations sampled north of the peri-Point Conception (PPC) region and the two populations sampled south of the PPC region (see Table 1), with 32.94% of the total variance being attributed to population differences between these two regions. Only 1.59% of the total variance is attributed to differences between populations within these two regions. Fixation indices further support a reduction in gene flow across the PPC region with FCT = 0.3294 indicating very great genetic differentiation . These data collectively support a regional population subdivision between the populations sampled on either side of the PPC region.
Mismatched distributions shown in Figure 4 indicate that populations north of the PPC region have been at equilibrium longer than populations in the south with a smaller mean number of pairwise differences (π) in northern populations (π = 5.6652±2.7449) as compared to those south of this geographic boundary (π = 8.7731±4.1338). While southern populations do not have an exact Poisson distribution, they do show a relatively unimodal mismatch distribution indicative of more recent population expansion . These data are consistent with the observed decrease in genetic variation in northern populations. Haplotype diversity (H) and nucleotide diversity (θπ) are significantly smaller in northern population as compared to populations found south of the PPC region (Table 2).
Populations north of the PPC region have been at equilibrium longer than populations to the south with a smaller mean number of pairwise differences (π) in southern populations (π = 8.7731±4.1338) as compared to those in the north (π = 5.6652±2.7449). Southern populations show a relatively unimodal mismatch distribution indicative of more recent population expansion.
A positive correlation between blade width and shell width was observed (Figure 5a). ANOVA of Phyllospadix blade width compared across all localities resulted in significant differences (p<<0.05) between four groups of populations (Figure 5b). Average blade widths were largest in HC (mean = 2.2886) and smallest in CR (mean = 1.2930). ANOVA of length:width ratios (LWR) identified significant differences (p<<0.00) between four groups (BR + TH + SR; CR; MB; HC) of populations that did not correspond to the same four population groupings (BR + MB; CR; HC; TH + SR) found for blade width alone (see Figure 5b–c). The longest and most narrow forms were found in the SR population instead of the CR population, which had the narrowest average blade widths.
. A) T. paleacea shell width is regressed against Phyllospadix blade (or stem) width across all localities resulting in a positive correlation. Scatter plots of B) Phyllospadix blade width and C) the degree of ovalness, measured as length:width ratios (LWR), compared across all localities are shown. Error bars show 95.0% confidence intervals. Note the lack of concordance between the patterns seen in B and C.
ANOVA and Tukey post hoc multiple comparisons of length:height ratios (LHR) across all localities indicated a statistically significant break between three groups (BR; CR + MB + HC; TH + SR) of populations (Figure 6a–b). BR had the largest LHR (p<<0.00) indicating that overall shell shape is characterized by elongated shell length and low apex height. The opposite was true for TH and SR populations, which had significantly smaller LHR (p<<0.00) indicating that shell shape is shorter in length with a relatively high apex. The three populations found between the most northern and southern extremes (CR, MB, and HC) were statistically indistinguishable form each other (p = 0.206), and were characterized by an intermediate shell shape.
A) A scatter plot of LHR across all localities is shown. Error bars show 95.0% confidence intervals. B) ANOVA p-values are given for all comparisons. Significance is estimated at the 0.05 level using the Tukey post hoc test. A statistically significant break is evident between three groups of populations: 1) BR; 2) CR, MB, HC; and 3) TH, SR.
Historic and phylogeographic patterns
A diverse patellogastropod fauna found in Tertiary fossil deposits from the Dominican Republic include species that were closely associated with marine plants . These records indicate the presence of marine seagrass limpets in the Caribbean by the late Miocene. The first record of a T. paleacea-like marine plant limpet in the subtropical Pacific comes from a Pleistocene deposit in the Sea of Cortez (Lindberg unpublished). As seagrass limpets expanded their range into the temperate North Pacific during the late Pliocene and early Pleistocene, populations in the subtropical Pacific and Caribbean went extinct . These fossil data indicate that the ancestral populations of both T. paleacea and T. depicta, the sister taxon of T. paleacea , originated in the Caribbean and moved through the Isthmus of Panama sometime before the closure of the isthmus 3.1–3.5 million years ago ,  and subsequently expanded their range into northern latitudes. The extant range of T. depicta is restricted from northern Baja to central California reflecting ancestral distributions.
AMOVA results indicate that a barrier to gene flow between northern and southern populations of T. paleacea exists somewhere between Santa Barbara and Monterey (i.e., the PPC region). While this region encompasses the well-known biogeographic boundary of Point Conception , , , this reduction in gene flow is not necessarily associated with Point Conception itself; more sampling would need to be done across the PPC region to confirm the exact location of the geographic barrier to gene flow . Historical patterns examined in association with AMOVA results indicate that northern population isolation resulted from a range expansion rather than a selective sweep. Evidence supporting a recent range expansion includes lower nucleotide diversity estimates north of Santa Barbara, the southern distribution of T. depicta, and concordant patterns seen in a variety of taxa that share a similar coastal distribution across the Northeastern Pacific , , .
From a phylogenetic perspective, haplotype relationships predicted from NJ and ML analyses further support the presence of a partial barrier to gene flow in the PPC region. As expected, due to the low levels of genetic variation present between our sampled populations, the tree resulting from ML analysis has less resolution than the NJ tree and neither have significant support values (Figure 2 & Figure S1). Nonetheless NJ and ML patterns of haplotype relationships indicate that an ancestral northern assemblage of populations has given rise to multiple clusters of extant southern populations independently. The presence of three (potentially more if ML results are considered) reciprocally monophyletic clades of haplotypes originating south of the PPC region nested within the paraphyletic clade of haplotypes originating north of the PPC region is indicative of at least three independent dispersal events originating from northern populations followed by rapid coalescence giving rise to the extant southern populations (Figure 2). In light of the known historical data that supports a range shift from the north to the south this pattern fits well with the fact that there is a persistent eddy extending from spring through summer south of Point Conception, which functionally makes Point Conception a one-way biogeographical boundary where larvae can only disperse north to south during the majority of the reproductive season for most marine species in this region , . Therefore, these flow patterns combined with more recent historical temperature shifts, which may have facilitated regional population contractions/expansions, could explain this pattern of repeated north to south dispersal. Given the available data we cannot rule out the possibility that T. paleacea populations may have gone extinct entirely south of Point Conception during the late Pliocene and early Pleistocene. If this was the case then these dispersal events represent range expansion into areas where T. paleacea had previously gone extinct. This raises the possibility that southern populations are being maintained as sink populations deriving from northern larval source populations. This scenario would have significant impact on conservation strategies being implemented in rocky intertidal environments along the California-Oregon coast.
Mechanisms regulating population structure
It has been generally accepted that seagrass canopies alter the hydrodynamic conditions of the space they occupy . Flume tank observations and field studies have demonstrated that seagrass beds reduce flow ,  and dampen vertical wave energy , . Recent evidence supports the long-standing hypothesis that this reduction in water flow acts as a mechanism to increases particle trapping , . The expectation is that the observed increase in particle trapping within seagrass beds should have significant effects on larval retention and subsequent patterns of recruitment for broadcast spawning taxa associated with seagrasses.
Given these findings from previous studies, the obligate association of T. paleacea with Phyllospadix would suggest increased levels of population structure due to decreased flow environments and subsequently increased larval retention within Phyllospadix beds. While we did not directly measure the level of larval dispersal ability associated with any given seagrass bed, our data indicate that the presence of any larval retention due to attenuated flow in seagrass beds is not enough to act as a population structuring force for populations of T. paleacea distributed along the California-Oregon coast. The lack of collection site-specific population structure indicates high levels of gene flow between sites. Given the lack of dispersal ability by adults, the possibility of this gene flow being a result of adult dispersal as opposed to larval dispersal is unlikely. While adult dispersal potential may exist via individuals hitchhiking on drifting clumps of seagrass, this has yet to be documented.
The data presented here lend support to the hypothesis that a biogeographic boundary within the PPC region is acting as the primary mechanism driving genetic population structuring in T. paleacea. This result is consistent with Avise's  hypothesis that concordance exists between biogeographic and intraspecific phylogeographic boundaries. This indicates a local decoupling of reproduction from larval recruitment  and in turn argues in support of pre-settlement larval availability as the primary population structuring force acting within these populations. These findings have important implications for the ecological importance of open-coast seagrass communities on larval recruitment. Specifically, these findings suggest that open-coast seagrass communities act primarily as larval sources and do not actively facilitate increased larval settlement.
Phylogeography and morphology
There is a clear decoupling between patterns of morphological variation and population structure. Shape change is shown in Figure 5c and indicates a shift from a low profile, elongate form in the south to a shorter and taller profile in the north. While there are three statistically significant clusters of morphotypes distributed across the sampled distribution of T. paleacea, the boundaries between these clusters do not correspond to the PPC region, the biogeographic boundary associated with the phylogeographic break between northern and southern populations (Figure 1). Furthermore, variability seen in Phyllospadix blade morphology (previous studies have also shown that Phyllospadix morphology systematically varies across its range ) cannot account for the morphological differences seen in T. paleacea populations given the lack of overlap between three of the four population groupings identified by ANOVA for LWR and blade width across localities (Figure 5b–c).
Two possible mechanisms might explain the pattern of morphological variation observed across the sampled distribution of T. paleacea. The first is natural selection followed by local adaptation, while the second is ecophenotypic plasticity in response to local environmental variability. If natural selection was acting to produce the morphological variation observed between populations, given the fact that Cytb is among the most rapidly evolving markers found within the mitochondrial genome of gastropods , we would expect to see a concordance between molecular and morphological patterns. Instead the presence of incomplete lineage sorting suggests that natural selection is not acting on the populations within each morphologically unique region, although the possibility remains that there has not been enough time for mitochondrial genes to reflect selective pressure .
The second possible mechanism is differential ecophenotypic expression as a response to habitat heterogeneity. A number of studies have identified similar morphological trends associated with local ecophenotypic responses to variable environments , , . Although currently there are few ecological data available for T. paleacea, making it difficult to determine the extent of ecophenotypic plasticity, studies on other intertidal snails have demonstrated that flow intensity can significantly alter foot size and shell morphology , ,  as can predation intensity , ,  and food supply . All of these factors may be contributing to the region-specific morphological variation observed in T. paleacea populations. Although there are limited food supply and predation data available to begin to address this question, transplant experiments could be used to test for plasticity and in turn determine the necessity for further ecological and/or molecular data.
Morphological variation within Phyllospadix may contribute to the differences observed between T. paleacea populations. Three morphological species of Phyllospadix (P. serrulatus, P. scouleri, and P. torreyi) occur along the coast of North America. Individuals of T. paleacea feed exclusively on the leaf epidermis of P. scouleri  and occasionally on P. torreyi . This feeding strategy has been attributed to the availability of proteins and absence of phenolic substances in blade epidermal cells of Phyllospadix . While studies have indicated strong support for the differentiation of P. serrulatus from P. scouleri and P. torreyi, few data support the separate species designation of P. scouleri and P. torreyi , . Additionally, intermediate forms of these two species have been identified  suggesting that hybridization may occur. However, comparison data do exist for leaf epidermal differences between these three named species , suggesting that epidermal cell shape and size differ between P. scouleri and P. torreyi. These data, combined with data that indicate latitudinal variability in P. scouleri and P. torreyi , may be associated with the morphological variation seen in T. paleacea populations living on these seagrasses.
Phyllospadix beds provide a partial defense against many larger benthic predators due to the inaccessibility of thin floating blades where T. paleacea is found. However, smaller predators, such as the sea star Leptasterias hexactis, are commonly found in these beds . Laboratory experiments conducted by Fishlyn and Phillips  demonstrated that T. paleacea individuals show no escape response as a result of contact with L. hexactis individuals. Instead, they suggest that T. paleacea is chemically camouflaged. If T. paleacea uses chemical camouflage as its primary defense against predators, it is unlikely that predation intensity could explain the region-specific variability seen in shell morphology. If we are to identify the mechanisms regulating ecophenotypic plasticity within T. paleacea, detailed studies examining the ecological role of T. paleacea in seagrass habitats and its ecophenotypic plasticity potential are needed.
This study identifies population structure of T. paleacea that is concordant with the biogeographic barrier at the PPC region in California. Historical data suggest that extant populations originated in southern subtropical regions and have made their way across this barrier only since the late Pliocene or early Pleistocene. Although larval dispersal was not directly measured, the pattern of population structuring observed indicates that seagrass communities, which are known to alter flow regimes within their environment, do not affect larval dispersal ability enough to impact on the connectivity of T. paleacea populations. Furthermore, morphological patterns are not concordant with the molecular patterns observed. These findings support the role of local habitat variability in maintaining differential ecophenotypic expression across the distribution of T. paleacea, suggesting that highly localized conservation efforts may not be as effective as large-scale conservation efforts in near shore marine environments with diverse life history components.
The resulting strict consensus tree topology using ML analysis. ML analysis resulted in two trees with the likelihood score 1542.74406 and few nodes with significant bootstrap support. Bootstrap values (4,168 replicates) are indicated above each branch. Southern clades are highlighted in red while northern clades are in black.
We would like to thank DB Wake, G Roderick and SA Nichols for their helpful comments and suggestions in the preparation of this manuscript. Also, we would like to thank SA Nichols, A Aronowsky, and CP Meyer for their long hours in the field and helpful discussions. The manuscript was considerably improved by comments from an anonymous reviewer. Any errors remain our own.
Conceived and designed the experiments: EB DRL. Performed the experiments: EB. Analyzed the data: EB DRL. Contributed reagents/materials/analysis tools: EB DRL. Wrote the paper: EB DRL.
- 1. Garstang W (1922) The theory of recapitulation: A critical restatement of the biogenetic law. Journal of the Linnean Society of London 35: 81–101.W. Garstang1922The theory of recapitulation: A critical restatement of the biogenetic law.Journal of the Linnean Society of London3581101
- 2. Thorson G (1946) Reproduction and larval development of Danish marine bottom invertebrates: with special reference to the planktonic larvae in the Sound (Øresund). Meddelelser fra Kommissionen for Danmarks Fiskeri- Og Havunder Soegelser 4: 1–523.G. Thorson1946Reproduction and larval development of Danish marine bottom invertebrates: with special reference to the planktonic larvae in the Sound (Øresund).Meddelelser fra Kommissionen for Danmarks Fiskeri- Og Havunder Soegelser41523
- 3. Harvey PH, Leigh BAJ, Smith JM, Nee S (1996) New uses for new phylogenies. xi. New York: Oxford University Press. 349 p.PH HarveyBAJ LeighJM SmithS. Nee1996New uses for new phylogenies.xiNew YorkOxford University Press349
- 4. Marko PB (2004) ‘What's larvae got to do with it?’ Disparate patterns of post-glacial population structure in two benthic marine gastropods with identical dispersal potential. Mol Ecol 13: 597–611.PB Marko2004‘What's larvae got to do with it?’ Disparate patterns of post-glacial population structure in two benthic marine gastropods with identical dispersal potential.Mol Ecol13597611
- 5. Miller KJ, Ayre DJ (2008) Population structure is not a simple function of reproductive mode and larval type: insights from tropical corals. J Anim Ecol 77: 713–724.KJ MillerDJ Ayre2008Population structure is not a simple function of reproductive mode and larval type: insights from tropical corals.J Anim Ecol77713724
- 6. Duda TFJ, Palumbi SR (1999) Population structure of the black tiger prawn, Penaeus monodon, among western Indian Ocean and western Pacific populations. Marine Biology (Berlin) 134: 705–710.TFJ DudaSR Palumbi1999Population structure of the black tiger prawn, Penaeus monodon, among western Indian Ocean and western Pacific populations.Marine Biology (Berlin)134705710
- 7. Hamm DE, Burton RS (2000) Population genetics of black abalone, Haliotis cracherodii, along the central California coast. Journal of Experimental Marine Biology & Ecology 254: 235–247.DE HammRS Burton2000Population genetics of black abalone, Haliotis cracherodii, along the central California coast.Journal of Experimental Marine Biology & Ecology254235247
- 8. Moberg PE, Burton RS (2000) Genetic heterogeneity among adult and recruit red sea urchins, Strongylocentrotus franciscanus. Marine Biology 136: 773–784.PE MobergRS Burton2000Genetic heterogeneity among adult and recruit red sea urchins, Strongylocentrotus franciscanus.Marine Biology136773784
- 9. Ayre DJ, Minchinton TE, Perrin C (2009) Does life history predict past and current connectivity for rocky intertidal invertebrates across a marine biogeographic barrier? Mol Ecol 18: 1887–1903.DJ AyreTE MinchintonC. Perrin2009Does life history predict past and current connectivity for rocky intertidal invertebrates across a marine biogeographic barrier?Mol Ecol1818871903
- 10. Palumbi SR (1992) Marine speciation on a small planet. Trends Ecol Evol 7: 114–118.SR Palumbi1992Marine speciation on a small planet.Trends Ecol Evol7114118
- 11. Grant AR (1938) A systematic revision of the genus Acmaea eschscholtz, including consideration of ecology and speciation. Berkeley: University of California, Berkeley. 55 p.AR Grant1938A systematic revision of the genus Acmaea eschscholtz, including consideration of ecology and speciation.BerkeleyUniversity of California, Berkeley55
- 12. Test AR (1945) Ecology of California Acmaea. Ecology 26: 395–405.AR Test1945Ecology of California Acmaea.Ecology26395405
- 13. Lindberg DR (2007) Patellogastropoda. In: Carlton JT, editor. Light and Smith's Manual Intertidal Invertebrates of the Central California Coast. Berkeley: University of California Press. DR Lindberg2007Patellogastropoda.JT CarltonLight and Smith's Manual Intertidal Invertebrates of the Central California CoastBerkeleyUniversity of California Press
- 14. Fritchman HK (1961) A study of the reproductive cycle in the California Acmaeidae (Gastropoda) Part II. Veliger 3: 95–101.HK Fritchman1961A study of the reproductive cycle in the California Acmaeidae (Gastropoda) Part II.Veliger395101
- 15. Fritchman HK (1962) A study of the reproductive cycle in the California Acmaeidae (Gastropoda) Part IV. Veliger 4: 134–140.HK Fritchman1962A study of the reproductive cycle in the California Acmaeidae (Gastropoda) Part IV.Veliger4134140
- 16. Kay MC, Emlet RB (2002) Laboratory spawning, larval development, and metamorphosis of the limpets Lottia digitalis and Lottia asmi (Patellogastropoda, Lottiidae). Invertebrate Biology 121: 11–24.MC KayRB Emlet2002Laboratory spawning, larval development, and metamorphosis of the limpets Lottia digitalis and Lottia asmi (Patellogastropoda, Lottiidae).Invertebrate Biology1211124
- 17. Strathmann MF (1987) Reproduction and development of marine invertebrates of the northern Pacific coast: data and methods for the study of eggs, embryos, and larvae. xii. Seattle: University of Washington Press. 670 p.MF Strathmann1987Reproduction and development of marine invertebrates of the northern Pacific coast: data and methods for the study of eggs, embryos, and larvae.xiiSeattleUniversity of Washington Press670
- 18. Dawson MN (2001) Phylogeography in coastal marine animals: a solution from California. Journal of Biogeography 28: 723–736.MN Dawson2001Phylogeography in coastal marine animals: a solution from California.Journal of Biogeography28723736
- 19. Hellberg ME (1996) Dependence of gene flow on geographic distance in two solitary corals with different larval dispersal capabilities. Evolution 50: 1167–1175.ME Hellberg1996Dependence of gene flow on geographic distance in two solitary corals with different larval dispersal capabilities.Evolution5011671175
- 20. Gilg MR, Hilbish TJ (2003) Patterns of larval dispersal and their effect on the maintenance of a blue mussel hybrid zone in southwest England. Evolution 57: 1061–1077.MR GilgTJ Hilbish2003Patterns of larval dispersal and their effect on the maintenance of a blue mussel hybrid zone in southwest England.Evolution5710611077
- 21. Kelly RP, Eernisse DJ (2007) Southern hospitality: a latitudinal gradient in gene flow in the marine environment. Evolution 61: 700–707.RP KellyDJ Eernisse2007Southern hospitality: a latitudinal gradient in gene flow in the marine environment.Evolution61700707
- 22. Keen AM, Doty CL (1942) An annotated check list of the gastropods of Cape Arago, Oregon. Oregon State Monographs - Studies in Zoology 3: 1–16.AM KeenCL Doty1942An annotated check list of the gastropods of Cape Arago, Oregon.Oregon State Monographs - Studies in Zoology3116
- 23. Horgan DA (1976) Population studies of the stenotopic limpet Notoacmea paleacea on its sea-grass host Phyllospadix torreyi. Western Society of Malacologists Annual Report 17–18.DA Horgan1976Population studies of the stenotopic limpet Notoacmea paleacea on its sea-grass host Phyllospadix torreyi.Western Society of Malacologists Annual Report1718
- 24. Boore JL, Brown WM (2000) Mitochondrial genomes of Galathealinum, Helobdella, and Platynereis: sequence and gene arrangement comparisons indicate that Pogonophora is not a phylum and Annelida and Arthropoda are not sister taxa. Mol Biol Evol 17: 87–106.JL BooreWM Brown2000Mitochondrial genomes of Galathealinum, Helobdella, and Platynereis: sequence and gene arrangement comparisons indicate that Pogonophora is not a phylum and Annelida and Arthropoda are not sister taxa.Mol Biol Evol1787106
- 25. Rambaut A (1996) Se-Al: Sequence alignment editor.A. Rambaut1996Se-Al: Sequence alignment editor.Available at http://evolve.zoo.ox.ac.uk/. Available at http://evolve.zoo.ox.ac.uk/.
- 26. Begovic E (2004) Population structuring mechanisms and diversification patterns in the Patellogastropoda of the North Pacific. Berkeley: University of California, Berkeley. 291 p.E. Begovic2004Population structuring mechanisms and diversification patterns in the Patellogastropoda of the North Pacific.BerkeleyUniversity of California, Berkeley291
- 27. Posada D, Crandall KA (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817–818.D. PosadaKA Crandall1998MODELTEST: testing the model of DNA substitution.Bioinformatics14817818
- 28. Sober E (2002) Instrumentalism, parsimony, and the Akaike framework. Philosophy of Science 69: S112–S123.E. Sober2002Instrumentalism, parsimony, and the Akaike framework.Philosophy of Science69S112S123
- 29. Swofford DL (1998) Phylogenetic analysis using parsimony (* and Other Methods). Sinauer Associates, Sunderland, Massachusetts. DL Swofford1998Phylogenetic analysis using parsimony (* and Other Methods).Sinauer Associates, Sunderland, Massachusetts
- 30. Mort ME, Soltis PS, Soltis DE, Mabry ML (2000) Comparison of three methods for estimating internal support on phylogenetic trees. Systematic Biology 49: 160–171.ME MortPS SoltisDE SoltisML Mabry2000Comparison of three methods for estimating internal support on phylogenetic trees.Systematic Biology49160171
- 31. Felsenstein J (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–791.J. Felsenstein1985Confidence limits on phylogenies: An approach using the bootstrap.Evolution39783791
- 32. Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479–491.L. ExcoffierPE SmouseJM Quattro1992Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data.Genetics131479491
- 33. Schneider S, Roessli D, Excoffier L (2000) Arlequin ver. 2.000: A software for population genetics data analysis. S. SchneiderD. RoessliL. Excoffier2000Arlequin ver. 2.000: A software for population genetics data analysis.Genetics and Biometry Laboratory, University of Geneva, Switzerland. http://lgb.unige.ch/arlequin. Genetics and Biometry Laboratory, University of Geneva, Switzerland. http://lgb.unige.ch/arlequin.
- 34. Tajima F (1983) Evolutionary relationship of DNA sequences in finite populations. Genetics 105: 437–460.F. Tajima1983Evolutionary relationship of DNA sequences in finite populations.Genetics105437460
- 35. Tajima F (1989) Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123: 585–595.F. Tajima1989Statistical method for testing the neutral mutation hypothesis by DNA polymorphism.Genetics123585595
- 36. Rogers AR, Harpending H (1992) Population growth makes waves in the distribution of pairwise genetic differences. Mol Biol Evol 9: 552–569.AR RogersH. Harpending1992Population growth makes waves in the distribution of pairwise genetic differences.Mol Biol Evol9552569
- 37. Slatkin M, Hudson RR (1991) Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing populations. Genetics 129: 555–562.M. SlatkinRR Hudson1991Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing populations.Genetics129555562
- 38. Lindberg DR (1982) A multi variate study of morphological variation of the limpet Notoacmea depicta and its synonyms Notoacmea gabatella and Notoacmea lepisma Gastropoda Acmaeidae. Bulletin Southern California Academy of Sciences 81: 87–96.DR Lindberg1982A multi variate study of morphological variation of the limpet Notoacmea depicta and its synonyms Notoacmea gabatella and Notoacmea lepisma Gastropoda Acmaeidae.Bulletin Southern California Academy of Sciences818796
- 39. Wright S (1978) Evolution and the genetics of populations, variability within and among natural populations. Chicago: University of Chicago Press. 580 p.S. Wright1978Evolution and the genetics of populations, variability within and among natural populations.ChicagoUniversity of Chicago Press580
- 40. Lindberg DR (1987) Evolution of the Patellogastropoda fauna of the Caribbean Sea during the Neogene. American Malacological Union Proceedings. DR Lindberg1987Evolution of the Patellogastropoda fauna of the Caribbean Sea during the Neogene.American Malacological Union ProceedingsKey West Florida. Key West Florida.
- 41. Coates AG, Obando JA (1996) The geologic evolution of the central American Isthmus. In: Jackson JBC, Budd AF, Coates AG, editors. Evolution and Environment in Tropical America. Chicago: Univ Chicago Press. pp. 21–56.AG CoatesJA Obando1996The geologic evolution of the central American Isthmus.JBC JacksonAF BuddAG CoatesEvolution and Environment in Tropical AmericaChicagoUniv Chicago Press2156
- 42. Collins TM, Frazer K, Palmer AR, Vermeij GJ, Brown WM (1996) Evolutionary history of northern hemisphere Nucella (Gastropoda, muricidae): molecular, morphological, ecological, and paleontological evidence. Evolution 50: 2287–2304.TM CollinsK. FrazerAR PalmerGJ VermeijWM Brown1996Evolutionary history of northern hemisphere Nucella (Gastropoda, muricidae): molecular, morphological, ecological, and paleontological evidence.Evolution5022872304
- 43. Briggs JC (1974) Marine zoogeography. New York: McGraw-Hill Book Co. 475 p.JC Briggs1974Marine zoogeography.New YorkMcGraw-Hill Book Co475
- 44. Burton RS (1988) Intraspecific phylogeography across the point conception biogeographic boundary. Evolution 52: 734–745.RS Burton1988Intraspecific phylogeography across the point conception biogeographic boundary.Evolution52734745
- 45. Valentine JW (1966) Numerical analysis of marine molluscan ranges on the extratropical northeastern Pacific shelf. Limnology & Oceanography 11: 198–211.JW Valentine1966Numerical analysis of marine molluscan ranges on the extratropical northeastern Pacific shelf.Limnology & Oceanography11198211
- 46. Dawson MN, Staton JL, Jacobs DK (2001) Phylogeography of the tidewater goby, Eucyclogobius newberryi (Teleostei, Gobiidae), in coastal California. Evolution 55: 1167–1179.MN DawsonJL StatonDK Jacobs2001Phylogeography of the tidewater goby, Eucyclogobius newberryi (Teleostei, Gobiidae), in coastal California.Evolution5511671179
- 47. Davis BJ, Demartini EE, McGee K (1981) Gene flow among populations of a teleost painted greenling Oxylebius pictus from Puget Sound to southern California USA. Marine Biology 65: 17–24.BJ DavisEE DemartiniK. McGee1981Gene flow among populations of a teleost painted greenling Oxylebius pictus from Puget Sound to southern California USA.Marine Biology651724
- 48. Hellberg ME, Balch DP, Roy K (2001) Climate-driven range expansion and morphological evolution in a marine gastropod. Science 292: 1707–1710.ME HellbergDP BalchK. Roy2001Climate-driven range expansion and morphological evolution in a marine gastropod.Science29217071710
- 49. Marko PB (1998) Historical allopatry and the biogeography of speciation in the prosobranch snail genus Nucella. Evolution 52: 757–774.PB Marko1998Historical allopatry and the biogeography of speciation in the prosobranch snail genus Nucella.Evolution52757774
- 50. Gaylord B, Gaines SD (2000) Temperature or transport? Range limits in marine species mediated solely by flow. American Naturalist 155: 769–789.B. GaylordSD Gaines2000Temperature or transport? Range limits in marine species mediated solely by flow.American Naturalist155769789
- 51. Wares JP, Gaines SD, Cunningham CW (2001) A comparative study of asymmetric migration events across a marine biogeographic boundary. Evolution 55: 295–306.JP WaresSD GainesCW Cunningham2001A comparative study of asymmetric migration events across a marine biogeographic boundary.Evolution55295306
- 52. Hemminga M, Duarte C (2000) Seagrass ecology. xi: Cambridge, UK; New York, NY: Cambridge University Press. 298.M. HemmingaC. Duarte2000Seagrass ecology.xiCambridge, UK; New York, NYCambridge University Press298
- 53. Gacia E, Granata TC, Duarte CM (1999) An approach to measurement of particle flux and sediment retention within seagrass (Posidonia oceanica) meadows. Aquatic Botany 65: 255–268.E. GaciaTC GranataCM Duarte1999An approach to measurement of particle flux and sediment retention within seagrass (Posidonia oceanica) meadows.Aquatic Botany65255268
- 54. Gambi MC, Nowell ARM, Jumars PA (1990) Flume observations on flow dynamics in Zostera marina eelgrass beds. Marine Ecology-Progress Series 61: 159–170.MC GambiARM NowellPA Jumars1990Flume observations on flow dynamics in Zostera marina eelgrass beds.Marine Ecology-Progress Series61159170
- 55. Fonseca MS, Cahalan JA (1992) A preliminary evaluation of wave attenuation by four species of seagrass. Estuarine Coastal & Shelf Science 35: 565–576.MS FonsecaJA Cahalan1992A preliminary evaluation of wave attenuation by four species of seagrass.Estuarine Coastal & Shelf Science35565576
- 56. Verduin JJ, Backhaus JO (2000) Dynamics of plant-flow interactions for the seagrass Amphibolis antarctica: field observations and model simulations. Estuarine Coastal & Shelf Science 50: 185–204.JJ VerduinJO Backhaus2000Dynamics of plant-flow interactions for the seagrass Amphibolis antarctica: field observations and model simulations.Estuarine Coastal & Shelf Science50185204
- 57. Duarte CM, Middelburg JJ, Caraco N (2005) Major role of marine vegetation on the oceanic carbon cycle. Biogeosciences 2: 1–8.CM DuarteJJ MiddelburgN. Caraco2005Major role of marine vegetation on the oceanic carbon cycle.Biogeosciences218
- 58. Avise JC (1994) Molecular markers, natural history and evolution. xiv. New York; London: Chapman & Hall. 511 p.JC Avise1994Molecular markers, natural history and evolution.xivNew York; LondonChapman & Hall511
- 59. Todd CD (1998) Larval supply and recruitment of benthic invertebrates: do larvae always disperse as much as we believe? Hydrobiologia 375-376: 1–21.CD Todd1998Larval supply and recruitment of benthic invertebrates: do larvae always disperse as much as we believe?Hydrobiologia375-376121
- 60. McMillan C, Phillips RC (1981) Morphological variation and isozymes of North American Phyllospadix potamogetonaceae. Canadian Journal of Botany 59: 1494–1500.C. McMillanRC Phillips1981Morphological variation and isozymes of North American Phyllospadix potamogetonaceae.Canadian Journal of Botany5914941500
- 61. Avise JC (2000) Phylogeography: the history and formation of species. Cambridge: Harvard University Press. JC Avise2000Phylogeography: the history and formation of species.CambridgeHarvard University Press
- 62. Palumbi SR (1984) Tactics of acclimation: morphological changes of sponges in an unpredictable environment. Science 225: 1478–1480.SR Palumbi1984Tactics of acclimation: morphological changes of sponges in an unpredictable environment.Science22514781480
- 63. Stearns SC (1989) The evolutionary significance of phenotypic plasticity: phenotypic sources of variation among organisms can be described by developmental switches and reaction norms. Bioscience 39: 436–445.SC Stearns1989The evolutionary significance of phenotypic plasticity: phenotypic sources of variation among organisms can be described by developmental switches and reaction norms.Bioscience39436445
- 64. Arsenault DJ, Marchinko KB, Palmer AR (2001) Precise tuning of barnacle leg length to coastal wave action. Proc Biol Sci 268: 2149–2154.DJ ArsenaultKB MarchinkoAR Palmer2001Precise tuning of barnacle leg length to coastal wave action.Proc Biol Sci26821492154
- 65. Kitching JA, Muntz L, Ebling FJ (1966) The ecology of Lough Ine. XV. The ecological significance of shell and body forms in Nucella. Journal of Animal Ecology 35: 113–126.JA KitchingL. MuntzFJ Ebling1966The ecology of Lough Ine. XV. The ecological significance of shell and body forms in Nucella.Journal of Animal Ecology35113126
- 66. Trussell GC, Etter RJ (2001) Integrating genetic and environmental forces that shape the evolution of geographic variation in a marine snail. Genetica 112-113: 321–337.GC TrussellRJ Etter2001Integrating genetic and environmental forces that shape the evolution of geographic variation in a marine snail.Genetica112-113321337
- 67. Vermeij GJ (1973) Morphological patterns in high inter tidal gastropods: adaptive strategies and their limitations. Marine Biology 20: 319–346.GJ Vermeij1973Morphological patterns in high inter tidal gastropods: adaptive strategies and their limitations.Marine Biology20319346
- 68. Appleton RD, Palmer AR (1988) Water-borne stimuli released by predatory crabs and damaged prey induce more predator-resistant shells in a marine gastropod. Proc Natl Acad Sci U S A 85: 4387–4391.RD AppletonAR Palmer1988Water-borne stimuli released by predatory crabs and damaged prey induce more predator-resistant shells in a marine gastropod.Proc Natl Acad Sci U S A8543874391
- 69. Palmer AR (1990) Effect of crab effluent and scent of damaged conspecifics on feeding growth and shell morphology of the Atlantic dogwhelk Nucella lapillus (L.). Hydrobiologia 193: 155–182.AR Palmer1990Effect of crab effluent and scent of damaged conspecifics on feeding growth and shell morphology of the Atlantic dogwhelk Nucella lapillus (L.).Hydrobiologia193155182
- 70. Kemp P, Bertness MD (1984) Snail shape and growth rates: evidence for plastic shell allometry in Littorina littorea. Proc Natl Acad Sci U S A 81: 811–813.P. KempMD Bertness1984Snail shape and growth rates: evidence for plastic shell allometry in Littorina littorea.Proc Natl Acad Sci U S A81811813
- 71. Barbour MG, Radosevich SR (1979) Carbon-14 uptake by the marine angiosperm Phyllospadix scouleri. American Journal of Botany 66: 301–306.MG BarbourSR Radosevich1979Carbon-14 uptake by the marine angiosperm Phyllospadix scouleri.American Journal of Botany66301306
- 72. Fishlyn DA (1976) Observations on the natural history and morphological variation of Notoacmea paleacea on Phyllospadix scouleri and P. torrey in northern California. Western Society of Malacologists Annual Report 16-17: DA Fishlyn1976Observations on the natural history and morphological variation of Notoacmea paleacea on Phyllospadix scouleri and P. torrey in northern California.Western Society of Malacologists Annual Report16-17
- 73. Kuo J, Stewart JG (1995) Leaf anatomy and ultrastructure of the North American marine angiosperm Phyllospadix (Zosteraceae). Canadian Journal of Botany 73: 827–842.J. KuoJG Stewart1995Leaf anatomy and ultrastructure of the North American marine angiosperm Phyllospadix (Zosteraceae).Canadian Journal of Botany73827842
- 74. den Hartog C (1970) The sea-grasses of the world. Amsterdam: North-Holland Pub. Co. C. den Hartog1970The sea-grasses of the world.AmsterdamNorth-Holland Pub. Co
- 75. Stewart JG, Myers B (1980) Assemblages of algae and invertebrates in southern California USA Phyllospadix dominated inter-tidal habitats. Aquatic Botany 9: 73–94.JG StewartB. Myers1980Assemblages of algae and invertebrates in southern California USA Phyllospadix dominated inter-tidal habitats.Aquatic Botany97394
- 76. Fishlyn DA, Phillips DW (1980) Chemical camouflaging and behavioral defenses against a predatory sea-star Leptasterias hexactis by 3 species of gastropods from the surfgrass Phyllospadix community. Biological Bulletin (Woods Hole) 158: DA FishlynDW Phillips1980Chemical camouflaging and behavioral defenses against a predatory sea-star Leptasterias hexactis by 3 species of gastropods from the surfgrass Phyllospadix community.Biological Bulletin (Woods Hole)158