Genes coding for 45S ribosomal RNA are organized in tandem arrays of up to several thousand copies and contain 18S, 5.8S and 26S rRNA units separated by internal transcribed spacers ITS1 and ITS2. While the rRNA units are evolutionary conserved, ITS show high level of interspecific divergence and have been used frequently in genetic diversity and phylogenetic studies. In this work we report on the structure and diversity of the ITS region in 87 representatives of the family Musaceae. We provide the first detailed information on ITS sequence diversity in the genus Musa and describe the presence of more than one type of ITS sequence within individual species. Both Sanger sequencing of amplified ITS regions and whole genome 454 sequencing lead to similar phylogenetic inferences. We show that it is necessary to identify putative pseudogenic ITS sequences, which may have negative effect on phylogenetic reconstruction at lower taxonomic levels. Phylogenetic reconstruction based on ITS sequence showed that the genus Musa is divided into two distinct clades – Callimusa and Australimusa and Eumusa and Rhodochlamys. Most of the intraspecific banana hybrids analyzed contain conserved parental ITS sequences, indicating incomplete concerted evolution of rDNA loci. Independent evolution of parental rDNA in hybrids enables determination of genomic constitution of hybrids using ITS. The observation of only one type of ITS sequence in some of the presumed interspecific hybrid clones warrants further study to confirm their hybrid origin and to unravel processes leading to evolution of their genomes.
Citation: Hřibová E, Čížková J, Christelová P, Taudien S, de Langhe E, Doležel J (2011) The ITS1-5.8S-ITS2 Sequence Region in the Musaceae: Structure, Diversity and Use in Molecular Phylogeny. PLoS ONE 6(3): e17863. https://doi.org/10.1371/journal.pone.0017863
Editor: Carles Lalueza-Fox, Institut de Biologia Evolutiva - Universitat Pompeu Fabra, Spain
Received: November 30, 2010; Accepted: February 11, 2011; Published: March 22, 2011
Copyright: © 2011 Hřibová et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work has been supported by the Grant Agency of the Academy of Sciences of the Czech Republic [grant awards no. IAA600380703 and KJB 500380901] and was performed in collaboration with the International Atomic Energy Agency [Research Agreement no. 13192]. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Ribosomal ribonucleic acid (rRNA) is the central component of ribosomes - small intracellular particles, which convert the information carried in the genetic code into protein molecules . In plant genomes, similar to other eukaryotes, the nuclear ribosomal RNA (rRNA) genes occur in thousands of copies and are organized in tandem arrays, typically clustered in two separate genomic loci. While the 5S rDNA locus encodes the 5S rRNA genes, the 45S rDNA locus contains genes for 18S, 5.8S and 26S rRNA, which are transcribed as a single unit and then spliced. The 18S, 5.8S and 26S rRNA genes are separated by internal transcribed spacers ITS1 and ITS2, and the 45S transcription units themselves are separated from each other by non-transcribed intergenic spacers (IGS), extending from the 3′ end of 26S rRNA to 5′ end of the 18S rRNA coding regions .
Although the ITS are not incorporated into the mature rRNA, they encode signals for proper processing of the rRNA transcripts, which itself depends on the secondary structure of ITS RNA . In contrast to the ITS1 spacer, secondary structures of functional ITS2 spacer as well as 5.8S rDNA sequences are highly conserved within plants , . ITS2 spacer forms a four helices structure with typical pyrimidine-pyrimidine bulge in helix II and the most conserved primary sequence includes the TGGT in the helix III –. The 5.8 S rDNA sequences contain three conserved motives in their nucleotide sequences that are essential for the correct folding of secondary structure , . The conserved secondary structure of ITS2 and 5.8S sequences makes it possible to identify divergent ITS paralogs with non-functional pseudogenes –.
The ribosomal genes arranged in tandem arrays are characterized by low intra-genomic diversity as a consequence of concerted evolution –. Individual copies of rRNA genes evolve more or less in unison and unequal crossing-over and high frequency of gene conversion are thought to be responsible for this –. The intraspecific and intra-population stability of rRNA locus on one hand and relatively fast evolution of ITS1 and ITS2, which are under lower selective pressure, made the ITS one of the most popular marker in phylogenetic studies , –. The analysis of the ITS was also employed to identify progenitors of hybrid species and to study the origin of polyploid species [e.g. 22]–.
However, the evolution of rRNA loci in hybrids and in allopolyploids in particular, may be complex and several evolutionary scenarios were observed which differ in the way the parental rRNA loci interact. In some species, such as Arabidopsis, Brassica or Silene, the divergent parental rDNA sequences remain conserved and evolve independently in hybrids without any interaction –. In other cases, such as in allopolyploids of Nicotiana or Dendrochilum, parental rDNA loci seem to recombine as judged from the occurrence of chimerical ITS sequences , . The third evolutionary pathway is a dominance of one type of rDNA sequence that may lead to partial or even complete homogenization of rDNA locus of the second parent , –. The dominant rDNA sequence may represent one of the parental rDNA sequence when one rDNA type is lost or eliminated or may be a chimeric sequence resulting from intergenomic recombination –. Obviously, if the concerted evolution is not complete, different types of paralogous and orthologous rDNA sequences may be present in the genome, often including silenced and non-functional rDNA sequences referred to as pseudogenes. The presence of pseudogenes in the phylogenetic data set may result in altered phylogenetic inference .
Banana (Musa spp.) is an important cash crop and staple food for millions people living in the humid tropics. Most of edible banana cultivars are seed-sterile diploid, triploid and tetraploid intra- and inter-specific hybrids, which contain various combinations of the A and B genomes originating from seed bearing progenitors M. acuminata Colla and M. balbisiana Colla, respectively . A few edible hybrids originated from crosses between M. acuminata and M. schizocarpa (S genome) and M. acuminata and presumably M. textilis (T genome) as well. The genus Musa belongs to the family Musaceae that includes three genera, Musa L., Ensete Horan and Musella C.Y. Wu ex H.W. Li, and has traditionally been divided into four sections based on morphology and basic chromosome number : Eumusa (2n = 2x = 22), Australimusa (2n = 2x = 20), Rhodochlamys (2n = 2x = 22) and Callimusa (2n = 2x = 18, 20). However, the traditional classification of the genus Musa has been questioned based on DNA analyses –. Wong et al.  proposed to compound sections Eumusa and Rhodochlamys into one and sections Australimusa and Callimusa into second one. Comparable observations on the similarity of Eumusa and Rhodochlamys on one hand and Australimusa and Callimusa on other hand were made by others , . Apart from this, the traditional taxonomy of Musa is also problematic for placement of newly described species , .
In addition to studies which employed AFLP, SSR and DArT markers , –, only two studies on genetic diversity and phylogenetic relationships in Musa involved the analysis of rDNA region, namely the length and specific restriction pattern of the ITS1-5.8S-ITS2 region. Nwakanma et al.,  noted that a specific restriction pattern of the ITS region enabled differentiation between diploid AA and BB genomes as well as between the triploid AAA intraspecific hybrid clones and those that carry the B genome (AAB and ABB). More recently, the nucleotide information of the ITS region was used to study relationships of wild species of Musaceae , . Nevertheless, there has been a lack of detailed knowledge on the ITS region in Musa and its potential for the analysis of genetic diversity and phylogenetic relationship remains unexplored.
In this work we set out to study in detail the rDNA ITS1-5.8S-ITS2 region in a total of 87 accessions of wild diploid species and triploid hybrid clones that represent all main taxonomic groups of the family Musaceae. We analyzed DNA sequences obtained either by classical dideoxy termination chain reaction or by massively parallel 454 sequencing with the aim to (1) thoroughly describe the nucleotide structure and diversity of the ITS region in Musa, Ensete and Musella; (2) compare the results obtained by different sequencing approaches; (3) analyze the presence of putative pseudogenic ITS sequence types and their influence on phylogenetic reconstructions; (4) assess the utility of ITS sequences for analysis of phylogenetic relationships within Musaceae and (5) use ITS to verify the genome constitution of inter- and intra-specific banana hybrids.
We have analyzed more than 2,500 nucleotide sequences from the ITS region of 87 accessions representing the family Musaceae, including 54 Musa diploids representing all sections of the Musa genus including four inter-subspecific hybrids, two Ensete and one Musella species (Table S1). In addition to the representatives of wild Musa species, ITS region of 30 hybrid banana clones was analyzed (Table S1). Three approaches were used to obtain ITS sequences. As the first step, the ITS region of all diploid genotypes was amplified using specific primers and four PCR products from individual PCR reactions of each accession were used for direct Sanger sequencing. Only 23 diploid genotypes produced readable ITS sequences with no polymorphism (Table S1), the remaining diploid accessions produced polymorphic ITS sequences. The ITS region of heterogeneous diploids and all hybrid clones was cloned and 15–85 DNA clones bearing ITS region were sequenced (Table S1). Finally, we have assessed the usefulness of massively parallel 454 sequencing for the analysis of the ITS loci.
Length variation, sequence diversity and GC content of the ITS region
In general, the length of ITS1 and ITS2 spacer varied from 216 to 223 bp and from 205 to 227 bp, respectively. A total length of ITS1-5.8S-ITS2 sequence region ranged from 578 bp (M. acuminata ‘Truncata’, ITC 0393) to 601 bp (M. mannii, ITC 1411) in all accessions, except for five representatives of section Australimusa: M. maclayi (ITC 1207), M. maclayi type Hung Si (ITC 0614), M. menei (ITC 1021), M. textilis (ITC 1072) and M. maclayi F. Muell, where the ITS region was 544 bp long. This significant length difference specific for these Australimusa entries was due to a 41 bp deletion in ITS1.
One of the highest sequence diversities within diploid species was observed in M. schizocarpa (ITC 0856, ITC 0846) (Figure S1A, B/sequence similarity matrix). The heterogenous representatives of Australimusa and Rhodochlamys sections contained more diverse ITS sequence region than the representatives of Eumusa. Within the hybrids, banana clones ‘Cachaco’ (ITC 0643) and ‘Dole’ (ITC 0767), representatives of Bluggoe subgroup (ABB genomes), showed the highest level of sequence divergence, while the other three Bluggoe clones showed relatively low level of sequence heterogeneity (data not shown). Sequence divergence of the entire ITS region was higher among the hybrids with genome constitution AAA, AxB and AxT than among corresponding diploid genotypes (Figure S2). On the other hand, AxS banana hybrid clones showed lower sequence divergence than the S genomes, which contained putative pseudogenes (Figure S2) GC content of ITS1 varied from 55.35 to 67.70% and was slightly lower than the GC content of ITS2 (56.11 to 70.97%).
The 5.8S rDNA sequence region had a conserved length of 155 bp or 154 bp, and its GC content varied from 49.68 to 57.48% and was significantly lower than the GC content in ITS1 and ITS2. Among all Musaceae species studied, the lowest GC content of the ITS region was identified in accession representing the section Australimusa ().
Secondary structure of ITS2 and 5.8S rDNA sequences in Musaceae and identification of pseudogenes
The secondary structure of ITS2 and 5.8S rDNA sequence regions was reconstructed for all accessions. ITS2 sequences formed specific four-helices structure with typical pyrimidine-pyrimidine bulge in helix II and the most conserved primary sequence included the TGGT in the helix III (Figure 1A, B). Secondary structure of 5.8S rDNA sequence was reconstructed under specific settings for base pairing (Figure 1C, D). Moreover, 5.8S rDNA sequences were checked for the presence of three conserved motives . The highly conserved sequence of 16 bp motif M1 (5′-CGATGAAGAACGTAGC-3′) is a part of two helices – helix B4 and B5 . One type of ITS sequence in heterogenous diploids M. acuminata ‘Tuu Gia’ (ITC 0610) and ‘Pisang Mas’ (ITC 0563) had a “T” at position 11 and 16 in the motif M1. Similarly, one type of ITS in M. acuminata ‘Truncata’ (ITC 0393) and M. schizocarpa (ITC 0856 and ITC 0846) had an “A” at position 12. Motif M2 (5′-GAATTGCAGAATCC-3′), previously described by Jobes and Thien  is 14 bp long and located in the loop and 10 bp long motif M3 (5′-TTTGAACGCA-3′) is a part of the B4 and B7 helices  (Figure 1C, D). Some ITS types of Musa diploids had a changes at positions 9 and 14 in motif M2 and changes at positions 7 and 8 in the motif M3. The nucleotide changes in conserved motives of 5.8S rDNA of diploid and hybrid accessions are summarized in Table S2. The information on GC content, presence of conserved motives in the 5.8S rDNA sequence and ability of ITS2 and 5.8S rDNA sequence to fold into a conserved secondary structure allowed us to identify putative pseudogenes (Table S2).
Positions within typical four-helices structures of ITS2 spacer of M. acuminata ‘Calcutta 4’ (A) and E. gilletii (B) are numbered every 20 nucleotides and helices are numbered I–IV. The common TGGT sequence motif in helix III is marked in red. The helices (B4–B8) in the conserved secondary structure of 5.8S rRNA gene sequences of M. acuminata ‘Calcutta 4’ (C) and E. gilletii (D) are numbered according to Wuyts et al. . Conserved motives (M1–M3) are marked in violet.
Comparison of the ITS1-5.8S-ITS2 sequence region obtained using different sequencing approaches
In addition to Sanger sequencing, we used massively parallel sequencing (454) to study the diversity of the ITS region within six diploid Musaceae accessions (Table S1) and to compare the data obtained using the two different sequencing approaches. Reconstructed contigs of ITS regions from 454 data were characterized by high read depth (Figure 2). Analysis of the 454 data confirmed that the ITS region is highly conserved in all six accessions. Moreover, the 454 consensus ITS sequences were highly similar to those obtained using Sanger sequencing.
The graphs show the read depth (RD; number of 454 reads that were assembled over individual position) along the contig sequence. Color coding: M. beccarii ITC 1070 - red; E. gilletii ITC1389 - violet; M. balbisiana ‘Pisang Klutug Wulung’ - blue; M. acuminata ‘Calcutta4’ ITC 0249 - light blue; M. ornata ITC 0637 - light green and M. textilis ITC 0539 - brown.
454 data of M. acuminata ‘Calcutta4’ resulted in one ITS sequence type that showed high similarity (99.67%) to the sequence obtained using direct Sanger sequencing - only two mismatches were found. ITS sequence region of M. balbisiana ‘Pisang Klutug Wulung’ obtained by 454 showed 99.83% similarity to the Sanger sequencing data, differing in one nucleotide insertion within the poly-G sequence region. The 454 sequencing of M. ornata and E. ventricosum resulted in two types of ITS sequence, showing 100% and 99.83% (single nucleotide substitution) similarity to the direct Sanger sequencing, output, respectively.
The highest number of 454 reads homologous to ITS region was obtained for M. beccarii, an observation which reflects higher copy number of 45S rRNA genes that are localized on three pairs of chromosomes in M. beccarii ITC 1070 . The remaining species analyzed by 454 sequencing have only one pair of chromosomes bearing the 45S locus. 454 sequencing resulted in two types of ITS sequence in M. beccarii. The first type showed 99.65% similarity to the sequence obtained by direct Sanger sequencing (2 nucleotide changes). The second one contained 8 nucleotide substitutions showing lower similarity (98.63%) to the Sanger sequencing data and the presence of nucleotide substitutions at potential methylation sites, indicated its pseudogenic nature.
Out of the six Musaceae species evaluated using the two different sequencing approaches, only M. textilis gave polymorphic ITS sequence after direct Sanger sequencing. Subsequent cloning and Sanger sequencing of 15 clones carrying the ITS region resulted in two different consensus ITS sequences (0539con1 and 0539con2) (Table S1). This observation was confirmed by 454 sequencing, which resulted in two types of ITS sequence differing in three nucleotide substitutions. Both 454-ITS types showed high similarity (99.49% and 99.67%) to 0539con1 ITS sequence. Second type of ITS sequence obtained after Sanger sequencing (putative pseudogene; Table S2) was not found in the 454 dataset despite the relatively high read depth of the ITS-454 data (RD = 149.6; Figure 2).
Position of the Musaceae clades with respect to closely related Zingiberales.
We have used two different methods to reconstruct phylogenetic relationships within Musaceae considering other closely related families of the order Zingiberales - Neighbor-Joining (NJ) and Bayesian inference (BI). The best-fit model of nucleotide substitution selected by jModeltest and implemented in Bayesian analysis was GTR+I+G. If putative pseudogenic ITS types were excluded from data set, NJ and BI resulted in fully resolved trees with high NJ bootstrap support and Bayesian posterior probabilities for all main clades (Figure 3). In these analyses, ITS1-ITS2 concatenated region of all diploid Musaceae accessions, including the four inter-subspecific diploid hybrids and closely related families from Zingiberales (dataset 1) was used for NJ constructed on Jukes-Cantor distance matrix and BI analysis performed in BEAST.
Neighbor-Joining (NJ) tree constructed from a Jukes-Cantor distance matrix of the concatenated region containing ITS1 and ITS2 spacer sequence. Bayesian inference (BI) analysis of the same data set was performed in BEAST v1.5.3 using GTR+I+G model of nucleotide substitution. Both trees are rooted at the midpoint and values below the branches indicate the bootstrap support of NJ analysis (A) and posterior probability of BI (B), respectively. Putative pseudogenes were not included in this analysis. Main clades and subclades were labeled by different colors that correspond to Figure 2. Ensete/Musella clade - violet; Australimusa/Callimusa - brown; Rhodochlamys - light green; B genomes - blue; A genomes - light blue and S genomes - pink.
The reconstructed phylogeny confirmed the monophyly of Musaceae and revealed three main clades within the family. The monophyly of Ensete and Musella was not supported as they shared the same clade. Species from the section Australimusa shared the same clade with the species from section Callimusa as did the species from sections Eumusa and Rhodochlamys (Figure 3). Moreover, the positions of the clade bearing Ensete and Musella species and that of Australimusa and Callimusa species was reverse as compared to the work of Liu et al. . Based on sequence analysis of 19 intron containing nuclear genes Christelová et al.  created a phylogenetic tree in which the position of the clade bearing Musella and Ensete genera was sister to the Musa genus and the Australimusa/Callimusa sections formed a separate subcluster within the Musa genus clade.
In case of Australimusa/Callimusa clade, M. coccinea appeared as a distinct species within the clade. All accessions of cultivated Fe'i banana, except ‘Utafan’ (ITC 0913) clustered with the Australimusa species and both, BI and NJ phylogenetic analysis supported their close relationships to M. textilis. Moreover, the Australimusa/Callimusa clade contained one accession from the Eumusa section - M. balbisiana 10852 (ITC 0094) (Figure 3). Within the Eumusa/Rhodochlamys clade, representatives of the B genome formed strongly supported subclade separated from other Eumusa and Rhodochlamys species. However, the B genome subclade contained also one representative of Fe'i bananas - ‘Utafan’ (ITC 0913). Another well separated subclade was formed by representatives of section Rhodochlamys. However, this strongly supported subclade contained one accession of section Australimusa – M. textilis ‘Née’ (ITC 0563), and one representative of Fe'i bananas – ‘Asupina’ (ITC 1027). Moreover, two Rhodochlamys accessions - M. ornata (ITC 0637) and M. velutina (ITC 0627) clustered with the A genome representatives, which supports close relationships of Eumusa and Rhodochlamys species. Within the representatives of M. acuminata, both phylogenetic analyses supported the close relationship of burmannica, burmannicoides, siamea and malaccensis subspecies.
The BI analysis revealed a well separated and strongly supported subclade of the S genome representatives (M. schizocarpa) together with M. acuminata ‘Niyarma yik’ (ITC 0269). In the NJ analysis, representatives of the S genome clustered not only with M. acuminata ‘Niyarma yik’ (ITC 0269), but also with two other representatives of A genome - M. acuminata ‘Malaccensis’ (ITC 0250) and M. acuminata ‘Galeo’ (ITC 0259). BI and NJ trees differed also in the position of M. nagensium. In the BI tree, M. nagensium shared the same clade with the B genome representatives, supporting their close relationship. On the contrary, M. nagensium formed a highly supported separate subclade in the NJ tree, indicating its phylogenetic isolation from other Eumusa species (Figure 3). Finally, the analysis revealed that presence of putative pseudogenic ITS data had no significant effect on positions of main clades in the BI and NJ phylogenetic trees. Nevertheless, the positions of some accessions were changed within the main clades, especially within the A genome representatives (Figure S3).
Evolutionary relationships of banana hybrid clones.
Thanks to the specific mode of the rDNA loci evolution, the ITS data can be used to analyze the genome composition and evolutionary relationships of hybrid plant species –, , . Here we present the first sequence data from the ITS region and its utilization for analysis of genome composition and evolutionary relationships in different banana hybrid clones. Both phylogenetic trees (BI and NJ) based on the ITS1-ITS2 concatenated region of dataset 2 (see Materials and Methods) contained cluster of ITS sequences corresponding to B genome well separated from the other genomes of Eumusa section. For example, banana clone ‘Saba’ ITC 1138, traditionally classified as ABB hybrid, contained three types of ITS sequence. One of them (1138con1) clustered with the ITS sequences corresponding to B genome and two other ITS types of ‘Saba’ (1138con2 and 1138con3) clustered with the A genome ITS types (Figure S4, Table S3). Similarly, M. jackeyi ITC 0851 which has been classified as AT hybrid contained three different types of ITS sequence region. One ITS type clustered together with the ITS corresponding to T genome (Australimusa/Callimusa clade) as well as the ITS sequence type (0851con3) identified as putative pseudogene. The third ITS sequence type of M. jackeyi (0851con2) clustered with the ITS types corresponding to A genome (Figure S4, Table S3). Similarly to dataset 1, most of the Rhodochlamys species formed a separated subclade. The cluster containing diploid representatives of the S genome and AS hybrids also contained ITS types from banana clones that were not classified as A×S hybrids (Figure S4). This supports close relationships of M. acuminata and M. schizocarpa. In comparison to the phylogenetic analysis using dataset 1, the position of the main clades within Musaceae in dataset 2 remained conserved in BI tree. Phylogenetic analysis using NJ of dataset 2 showed reversed position of Ensete/Musella and Australimusa/Callimusa clades (Figure S4).
BI and NJ phylogenetic study revealed that most of the banana hybrid clones contained conserved parental ITS sequence types. This information could facilitate rapid determination of genome composition of newly created hybrid clones. However, not all of the evaluated hybrid clones carried conserved parental ITS types (Figure S4, Table S3). The hybrid clones with the reported AAB genome constitution (‘Maritú’ ITC 0639, subgroup Iholena and ‘3 Hands Planty’ ITC 1132, subgroup Plantain) and ABB (‘Cachaco’ ITC 0643, subgroup Bluggoe) did not contain ITS sequence corresponding to the B genome. These results suggest partial homogenization of the rDNA locus in the clone ‘Maritú’ which contained two different ITS sequence types where one of them has putative pseudogenic character. On the other hand the hybrid clones ‘3 Hands Planty’ ITC 1132 (subgroup Plantain) and ‘Cachaco’ ITC 0643 (subgroup Bluggoe) contained four and six ITS sequence types, respectively. The presence of chimeric ITS sequences with higher number of putative pseudogenic sequences in these cases can be due to recombination as described in other plant hybrids , . In the Bluggoe subgroup, two other hybrid clones - ‘Cachaco Enano’ ITC 0632 and ‘Kivuvu’ ITC 0157 exhibited partial concerted evolution when the ITS sequence of A genome was not found in their genomes. Banana clone ‘Butuhan’ ITC 1074, reported as BB×TT hybrid contained only ITS sequence corresponding to B genome. Similarly, clone ‘Tonton Kepa’ ITC 0822 reported as AS hybrid contained only S genome ITS sequence (Figure S4, Table S3).
The ITS1-5.8S-ITS2 sequence region is one of the most popular loci used in molecular phylogenetic studies . Despite this, the ITS region has not been analyzed in detail in the genus Musa and has only been used once to examine its phylogenesis . However, the study suffered from the experimental approach as it was based solely on direct sequencing of the ITS locus, and the authors did not consider sequence homogeneity/heterogeneity of the region. As the potential of the ITS analysis in the taxonomy of Musaceae remains unexplored, we chose to study the structure and diversity of the ITS1-5.8S-ITS2 region in wild species of Musa as well as in cultivated banana clones. We evaluated different sequencing approaches including the next generation sequencing technology and their impact on the phylogenetic reconstruction.
ITS length variation, secondary structure and identification of pseudogenes
Recent studies revealed that divergent ITS paralogs may contain non-functional pseudogenes – whose presence in phylogenetic data sets can result in altered phylogenetic inferences . ITS pseudogenes were identified experimentally using transcript analysis . Alternatively, in silico methods such as the analysis of nucleotide divergence, insertions and deletions (length of the ITS sequence region), analysis of methylation-induced substitution pattern, folding of conserved secondary structures and sequence free energy were used . Due to the lack of transcript data, we evaluated ITS length, GC content and secondary structure of ITS2 and 5.8S rDNA regions. The total length of ITS regions as well as the length of putative functional ITS1 and ITS2 spacers agreed with the observations in other angiosperms . However, we have identified polymorphic ITS regions in most of the Musa accessions and it is possible that at least some of the polymorphisms were due to the occurrence of pseudogenic sequences. In agreement with other studies , the putative pseudogenic ITS sequences varied in length of ITS1 spacer, had lower GC content, did not contain conserved motives in the 5.8S rDNA sequence and/or lost the ability to fold into functional ITS2 and/or 5.8S rDNA secondary structures.
Intra-individual polymorphism and evolution of ITS
Despite the concerted evolution of rDNA, ITS polymorphism within individuals is quite common , , ,  and may be due to various mechanisms. A single diploid genome contains divergent paralogs when the speciation is faster than concerted evolution or when paralogous rDNA sequences are present in non-homologous loci in the heterozygous species. Our results suggest incomplete concerted evolution in some wild diploid banana species, in which we revealed increased levels of ITS polymorphism and divergent paralogs of ITS sequences. The latter might be formed as a consequence of inter- and/or intra-genomic duplication events. Alternatively, the polymorphic character of ITS region in some wild diploids could be due to inter-subspecific hybrid origin and asexual reproduction, which bypasses meiotic recombination.
Owing to unclear origin of hybrid banana varieties and their clonal character, it is difficult to reconstruct the evolution of the 45S rDNA locus in Musa. Our results suggest that all three modes of evolution were involved in polyploid hybrid clones. Most of the interspecific banana hybrids presumably originating from crosses M. acuminata×M. balbisiana, M. acuminata×M. schizocarpa and M. acuminata×M. textilis contained conserved ITS sequences of both parents, indicating incomplete concerted evolution of rDNA loci. No or limited homogenization of ITS sequence was described for allopolyploid genomes of European dogroses, where most of the genomes are excluded from meiotic recombination . The independent evolution of parental rDNA sequences in banana hybrid clones, which is probably due to the absence of sexual reproduction, could facilitate determination of genomic constitution of new hybrid banana cultivars. However, the fact that presence of chimeric ITS sequences and/or partial concerted evolution was observed in some hybrids may limit the potential of this approach. These hybrids show various degree of residual fertility and it is possible that their evolution involved episodes of sexual reproduction as suggested by the backcross hypothesis . Our results indicate a complete concerted evolution in two banana clones: ‘Butuhan’ (BB×TT) and ‘Tonton Kepa’ (AA×SS) in which only ITS sequence of B genome and S genome was present, respectively. However, this observation may be explained either by a more complicated evolution, which included backcrossing to one of the parental species , or a non-hybrid origin. Clearly, the genomic constitution of both clones needs to be confirmed using additional molecular analysis.
Comparison of ITS region obtained using different sequencing approaches
Plant species and hybrid and polyploid ones in particular, often contain several rDNA loci with multiple divergent ITS sequence types. Multiple ITS sequence types may originate by different evolutionary mechanisms and their utilization in molecular phylogeny may shed light on polyploid ancestry, genome relationships, genomic constitution and evolution of hybrid species –, , .
The ITS sequence complexity (diversity) may lead to errors in phylogenetic interference due to PCR bias and/or PCR drift [reviewed in 17]. To check the effect of ITS heterogeneity, we compared ITS sequence data obtained by classical approach (sequencing PCR products and/or PCR/cloning strategy) with the data obtained by massively parallel 454 sequencing . We obtained whole genome 454 data from five Musa and one Ensete species with the length of sequencing reads sufficient to assembly individual ITS sequence types without PCR/cloning strategy. The ITS sequence data obtained using classical approach were highly similar to those obtained using 454 and resulted in the same phylogeny. However, the 454 data obtained in M. beccarii (ITC 1070) indicated the presence of two ITS sequence types while direct Sanger sequencing resulted in one readable ITS sequence with no polymorphism. This could be a consequence of PCR bias, when a single repeat type is preferentially amplified. Other exception was M. textilis (ITC 0539), in which one sequence type of ITS obtained after Sanger sequencing was not found in the 454 dataset despite the relatively high read depth. This outcome suggests some modification of the ITS sequence during the classical PCR/cloning methodology and indicate that massively parallel sequencing may be a preferable approach to study 45S rDNA sequence region as well as the other complex gene families.
Phylogenetic reconstruction using ITS
In order to analyze the position of the family Musaceae and its main clades and subclades within the order Zingiberales, we studied the ITS sequences in other families of Zingiberales (Strelitziaceae, Lowiaceae, Heliconiaceae and Costaceae). In line with the results of Liu et al. , who studied ITS and chloroplast loci, our analyses showed monophyletic origin of Musaceae. Both BI and NJ phylogenetic trees showed close relationships of species belonging to sections Australimusa and Callimusa and sections Eumusa and Rhodochlamys, thus confirming previous findings , , , , . At the same time, both trees showed reverse positions of the Ensete/Musella clade and Australimusa/Callimusa clade, probably due to the use of only one genomic locus (ITS) in the present work. Among the Eumusa/Rhodochlamys species, we revealed a distinct position of M. balbisiana, which has traditionally been placed within the Eumusa, and most of Rhodochlamys species. This suggest earlier separation of the representatives of B genome from the A genomes and Rhodochlamys representatives in the evolution of Musaceae and is on line with the divergence time estimates of Christelová et al. . The placement of M. acuminata and M. balbisiana into two distinct subclades within Eumusa/Rhodochlamys clade was suggested by Li et al.  and close relationship of M. acuminata (section Eumusa) with M. laterita and M. ornata (both section Rhodochlamys), was also observed earlier , , . Separation of Eumusa and Rhodochlamys sections was questioned already by Cheesman  and Shepherd  and the present as well as earlier results obtained with molecular tools indicate close relationships of the representatives from both sections , , , .
In our study, three accessions classified as representatives of section Australimusa (M. textilis ‘Née’ ITC 0563 and Fe'i bananana clones ‘Utafan’ ITC 0913 and ‘Asupina’ ITC 1027) were found clustering with B genomes subclade or Rhodochlamys subclade. Similar situation was observed for M. balbisiana 10852 (ITC 0094) which grouped with Australimusa (see Results and Figure 3). This unexpected clustering could be due to mislabeling during their collection and/or during in vitro propagation in the gene bank. Alternatively, these accessions could be interspecific hybrids and/or their backcross progenies .
Despite a range of studies , , , , taxonomic and phylogenetic positions of some taxa within the Musaceae are still unclear and/or controversial. One of them is Musella, which has been classified based on morphology as a separate genus of Musaceae . The present study indicates close relationship of Musella and genus Ensete and does not confirm the monophyly of Musella. The same observation was made by molecular phylogenetic analysis based on the ITS and clDNA sequences ,  as well as work based on genic sequences .
The problematic phylogenetic position was also reported for the two representatives of section Callimusa, which differ by basic chromosome number - M. beccarii (2n = 2x = 18) and M. coccinea (2n = 2x = 20) . Our study supports close relationship of Callimusa with the section Australimusa (2n = 2x = 20), where M. coccinea is relatively distinct from other accessions. The distinctiveness of M. coccinea is supported by morphological characters , AFLP analysis  and by the ITS and clDNA analysis of Li et al. . In contrast to Liu et al. , our data do not support close relationship of M. beccarii and M. maclayi and indicate close relationships of cultivated Pacific Fe'i bananas to M. textilis and M. maclayi, while not confirming genetic vicinity of M. textilis and M. balbisiana.
An important outcome of this study is the identification of putative pseudogenic ITS paralogs in some banana diploid representatives and most of banana hybrid clones. Pseudogenes are expected to evolve independently at different rates than their functional counterparts and can accumulate mutations. This may lead to random clustering across phylogenetic trees and result in incorrect or incongruent phylogenies , , . However, Ochieng et al.  found that the ITS pseudogenes can recover more corroborated phylogeny of closely related species when functional paralogs suffer selective constraints and provide too low variation. In this work we demonstrate that the presence of putative pseudogenic ITS sequences has no significant effect on the structure of main clades in the phylogenetic analysis of Musaceae. On the other hand, their presence may change the position of subclades (Figure S3). These results show that pseudogenic ITS sequences should be used with caution when analyzing phylogenesis in Musa. As both Liu et al. and Li et al. ,  used only direct Sanger sequencing, differences in the placement of some diploid species in their phylogenetic trees as compared to our results could be due to the inability to detect different ITS sequences types.
The present study provides the first insights into the nucleotide structure and diversity of the ITS1-5.8S-ITS2 region in Musaceae and reveal the presence of more than one type of the ITS sequence within some Musa species and hybrid banana clones. We show that it is necessary to use clone-based sequencing strategy for most of the species within Musaceae and identify putative pseudogenic ITS sequences that may have negative effect on phylogenetic reconstruction. We show that both Sanger and 454 sequencing lead to similar phylogenetic inferences. Phylogenetic analysis based on the ITS sequences does not support Musella as a distinct genus within the Musaceae and confirms close phylogenetic relationship of the species from sections Australimusa and Callimusa, and Eumusa and Rhodochlamys, where the B genome representatives form a distinct subclade. The reversed positions of the Ensete/Musella clade and Australimusa/Callimusa clade in the phylogenetic trees suggest that phylogenetic reconstruction based on a single genomic locus (ITS) may lead to inconsistent phylogenetic inference. On the other hand, the use of ITS locus in phylogenetics has proven powerful in reconstructing relationships on lower taxonomic levels (groups and subgroups). Most of the intraspecific banana hybrids analyzed in this work contain conserved parental ITS sequences, indicating incomplete concerted evolution of rDNA loci. Independent evolution of parental rDNA loci in hybrids enables verification of genomic constitution of hybrids using ITS. The observation of only one type of ITS sequence in some of the presumed interspecific hybrid clones warrants further study to confirm their hybrid origin and evolution of their genomes.
Materials and Methods
In vitro rooted plants of most of the Musa and Ensete species used in the study as well as the hybrid clones (Table S1) were obtained from the International Transit Centre (ITC, Katholieke Universitiet, Leuven, Belgium). M. balbisiana ‘Pisang Klutug Wulung’ was obtained from Dr. François Côte (CIRAD, Guadeloupe) as rooted plants. Seeds of M. nagensium and M. maclayi F. Muell were obtained from Prof. Markku Häkkinen (Botanic Garden, University of Helsinki, Finland) and plants of Musella lasiocarpa were purchased from a commercial nursery. Plants and/or seeds were transferred to soil and grown in a greenhouse.
Amplification, cloning and Sanger sequencing of ITS1-5.8S-ITS2 region
Genomic DNA of all Musa, Ensete and Musella species used for the ITS analysis was isolated from fresh cigar leaves using the Invisorb Spin Plant Mini Kit (Invitek, Berlin, Germany) following the manufacture's recommendations. The ITS region was amplified from the genomic DNA using PCR with specific primers ITS-L and ITS-4 . PCR reaction mix consisted of 10 µg genomic DNA, 1.5 mM MgCl2, 0.2 mM dNTPs, 1 µM primers ITS-L and ITS-4, 1× PCR buffer and 2 U/100 of Dynazyme™ II DNA polymerase (Finnzymes, Espoo, Finland). Amplification was performed using PTC-200 thermal cycler (BIO-RAD, Hercules, USA), with the following conditions: 94°C for 5 min (1 cycle), 94°C for 50 s, 52°C for 50 s, 72°C for 50 s (35 cycles) and 72°C for 10 min (1 cycle), and PCR products were resolved in 1.5% agarose gels.
In the first experiment, PCR products of all diploid accessions were purified using ExoSAP-IT® (USB, Cleveland, USA) according to the manufacturer's instructions and used for direct sequencing. PCR products containing polymorphic ITS sequences were cloned into TOPO vector and transformed into E. coli electrocompetent cells (Invitrogen Life Technologies, Carlsbad, USA). For each heterogenous accession, 15 to 85 cloned PCR products were sequenced. Sequencing was carried out using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, USA) according to the manufacturer's instructions and run on ABI 3730xl DNA analyzer (Applied Biosystems). Nucleotide sequences were edited using Staden Package .
454 sequencing and data analysis
Genomic DNA of M. acuminata ‘Calcutta4’ (ITC 0249), M. balbisiana ‘Pisang Klutug Wulung’, M. ornata (ITC 0637), M. textilis (ITC 0539) and M. beccarii (ITC 1070) was prepared from nuclei isolated from healthy young leaf tissues according to Zhang et al. . Intact nuclei of E. gilletii (ITC 1389) were isolated by flow cytometric sorting following the protocol of Šafář et al. . Isolated nuclei were incubated with 40 mM EDTA, 0.2% SDS and 0.25 µg/µl proteinase K for 5 hrs at 37°C, and DNA was purified by phenol/chloroform precipitation.
454 shotgun sequencing libraries were prepared by the GS Titanium library preparation kit (Roche Diagnostics, Rotkreutz, Switzerland). Single-stranded libraries were quantified by a qPCR assay  and processed utilizing the GS Titanium SV/LV emPCR and XLR70 sequencing kits according to the manufacturer's instructions (Roche Diagnostics). Sequencing was performed on a half 70×75 picotiter plate for each Musa and Ensete species. Sequencing reads with the similarity to ITS1-5.8S-ITS2 region and 45S rRNA genes, respectively were identified using RepeatMasker  and the consensus sequences of the ITS1-5.8S-ITS2 region were assembled using cap3 program .
The read depth (RD) of the ITS sequence region in all six accessions was estimated according to Macas et al.  using home-made perl script and plots showing RD along the ITS locus (Figure 2) were created using the CALC program (http://www.openoffice.org/product/calc.html).
ITS sequence analysis and secondary structure reconstruction
Nucleotide sequences obtained from both sequencing approaches as well as the nucleotide sequences of ITS regions of other known species from the order Zingiberales that were downloaded from the GenBank database (Table S4), were edited using Staden Package  and the sequence boundaries of the spacers were determined by comparison to known rice ITS sequence (GB code: AF169230). Multiple sequence alignments of the ITS regions were done using MUSCLE v3.70+fix1-2  and graphically shown in SeaView v4.2.1 . Multiple sequence alignment was used in accessions with polymorphic ITS sequences to identify individual ITS types, which were then used for further analysis. Nucleotide diversity (π) of ITS region was estimated in MEGA 4 .
GC content and sequence identity of ITS regions within heterogenous accessions was calculated using BioEdit (http://www.mbio.ncsu.edu/BioEdit/bioedit.html). Secondary structures of ITS2 region were predicted using mFOLD program version 3.2 ,  at the default temperature (37°C) as well as at the 25°C and XRNA program version 1.2.0 Beta (http://rna.ucsc.edu/rnacenter/xrna/xrna_download.html). No differences in folding at both temperatures were observed. The 5.8S rDNA sequences were checked for the presence of the three conserved angiosperm motifs according to Harpke and Peterson . The secondary structure of 5.8S rRNA gene was reconstructed under specific settings for base pairing in XRNA program: for helix B4, F 38 98 3; helix B5, F 41 54 3; helix B6, F 62 89 3; helix B7, F 103 111 3; and for helix B8, F 112 135 4 and F 119 128 3.
Consensus sequences of the ITS regions have been deposited in the GenBank (accession numbers: FR727838–FR727974) as well as on our web site (http://olomouc.ueb.cas.cz/phylogeny-musaceae) where they are freely available.
Two different datasets were generated: in order to analyze the position of the clades within Musaceae with respect to other phylogenetic groups of Zingiberales, dataset 1 comprised ITS1-ITS2 concatenated regions of all diploid Musaceae accessions as well as the known ITS1-ITS2 sequences of closely related families Strelitziaceae, Lowiaceae, Heliconiaceae and Costaceae ,  (Table S4). Dataset 2 comprised ITS1-ITS2 sequences of all Musaceae accessions including hybrids and was used to analyze the genome composition and evolutionary relationships of hybrid banana species. All datasets were analyzed using Neighbour joining (NJ) and Bayesian inference (BI). Neighbour joining (NJ) method was carried out using SplitsTree4 v4.1.11  based on the Jukes-Cantor and uncorrected p-distances. Non-parametric bootstrapping with 1000 pseudoreplicates was performed to assess the nodal support.
The most appropriate model of nucleotide substitution for each dataset was determined using jModelTest v0.1.1 . Likelihood calculations were carried out using integrated PhyML  for 11 substitution schemes (88 different models) and the model selected under Akaike information criterion (AIC)  was implemented in the BI settings. Bayesian inference analysis was performed in BEAST v1.5.3  with four independent Markov Chain Monte Carlo (MCMC) runs, starting from randomly chosen topologies. The MCMC were run for 10,000,000 generations, data were sampled every 1,000 generations. Log-file outputs were inspected in Tracer  to confirm the correct convergence of the analysis. Treefiles from individual MCMC runs were subsequently combined by LogCombiner . The first 25% of the generations were discarded as the burn-in, the maximum clade credibility tree and corresponding posterior probabilities were calculated using TreeAnnotator . Phylogenetic trees were drawn and edited using FigTree (http://tree.bio.ed.ac.uk/software/figtree/) program.
Sequencing data analysis and statistical analysis described above were performed on LINUX systems.
An example of similarity matrix of heterogenous ITS sequences of M. jackeyi (A) and M. schizocarpa (B).
A box plot showing sequence diversities (π) within Musa representatives with different genome constitution and hybrid clones. The inter-subspecific diploids were not included in the analysis. Three diploid species which gave unexpected clustering in the phylogenetic tree (see Results and Discussion) were included in the data set according the results of NJ and BI analysis. * Only three representatives of S genome were included in our study. Two of them contained 3 ITS types including putative pseudogenic sequences and show the highest nucleotide difference among the analyzed species.
A BI and NJ phylogenetic trees of all ITS types including putative pseudogenes, obtained in diploid Musaceae accessions and selected species of closely related families Strelitziaceae, Lowiaceae, Heliconiaceae and Costaceae based on the ITS1-ITS2 sequence region. NJ tree was constructed from a Jukes-Cantor distance matrix and BI analysis of the same data set was performed in BEAST v1.5.3 using GTR+I+G model of nucleotide substitution. Values below the branches indicate the bootstrap support of NJ analysis and posterior probability of BI, respectively. Main clades and subclades are labeled by different colors as used in Figure 3 and putative pseudogenic types of ITS sequences are marked by asterisks.
A BI and NJ phylogenetic analysis trees of all evaluated Musaceae accessions including banana hybrids and selected species of the closely related families Strelitziaceae, Lowiaceae, Heliconiaceae and Costaceae based on the ITS1-ITS2 sequence region. NJ tree was constructed from a Jukes-Cantor distance matrix and BI analysis of the same data set was performed in BEAST v1.5.3 using GTR+I+G model of nucleotide substitution. Values below the branches indicate the bootstrap support of NJ analysis and posterior probability of BI, respectively. Main clades and subclades are labeled by different colors as used in Figure 3 and putative pseudogenic types of ITS sequences of hybrids are marked by asterisks.
Diploid representatives of the family Musaceae and Musa hybrid clones used in the study. Diploid representatives that are probably inter-subspecific hybrids  are marked by asterisk.
Sequence characteristics of ITS1-5.8S-ITS2 regions in Musaceae.
Table showing genome composition of hybrid clones analyzed in this study and corresponding ITS sequence types identified after NJ and BI analysis.
We thank to Jiří Macas for providing a perl script, Ms. Radka Tušková for excellent technical assistance, and Prof. Markku Häkkinen, Dr. François Côte and Ir. Ines van den Houwe for providing the plant material.
Conceived and designed the experiments: EH JD. Performed the experiments: EH JC PC ST. Analyzed the data: EH. Contributed reagents/materials/analysis tools: EdL EH ST. Wrote the paper: EH JD EdL.
- 1. Gilbert W (1986) Origin of life: the RNA world. Nature 319–618.W. Gilbert1986Origin of life: the RNA world.Nature319618
- 2. Rogers SO, Bendich AJ (1987) Ribosomal RNA genes in plants: variability in copy number and in intergenic spacer. Plant Mol Biol 9: 509–520.SO RogersAJ Bendich1987Ribosomal RNA genes in plants: variability in copy number and in intergenic spacer.Plant Mol Biol9509520
- 3. Hillis DM, Dixon MT (1991) Ribosomal DNA – molecular evolution and phylogenetic inference. Q Rev Biol 66: 410–453.DM HillisMT Dixon1991Ribosomal DNA – molecular evolution and phylogenetic inference.Q Rev Biol66410453
- 4. Hershkovitz MA, Zimmer EA (1996) Conservation patterns in angiosperm ITS2 sequences. Nucleic Acids Res 24: 2857–2867.MA HershkovitzEA Zimmer1996Conservation patterns in angiosperm ITS2 sequences.Nucleic Acids Res2428572867
- 5. Jobes DV, Thien LB (1997) A conserved motif in the 5.8S ribosomal RNA (rRNA) gene is a useful diagnostic marker for plant internal transcribed spacer (ITS) sequence. Plant Mol Biol Rep 15: 326–334.DV JobesLB Thien1997A conserved motif in the 5.8S ribosomal RNA (rRNA) gene is a useful diagnostic marker for plant internal transcribed spacer (ITS) sequence.Plant Mol Biol Rep15326334
- 6. Coleman AW (2007) Pan-eukaryote ITS2 homologies revealed by RNA secondary structure. Nucleic Acids Res 35: 3322–3329.AW Coleman2007Pan-eukaryote ITS2 homologies revealed by RNA secondary structure.Nucleic Acids Res3533223329
- 7. Schultz J, Stefanie M, Gerlach D, Muller T, Wolf M (2007) A common core of secondary structure of the internal transcribed spacer 2 (ITS2) throughout the Eukaryota. RNA 11: 361–364.J. SchultzM. StefanieD. GerlachT. MullerM. Wolf2007A common core of secondary structure of the internal transcribed spacer 2 (ITS2) throughout the Eukaryota.RNA11361364
- 8. Wolf M, Achtziger M, Schultz J, Dandekar T (2005) Homology modeling revealed more than 20,000 rRNA internal transcribed spacer 2 (ITS2) secondary structures. Bioinformatics 11: 1616–1623.M. WolfM. AchtzigerJ. SchultzT. Dandekar2005Homology modeling revealed more than 20,000 rRNA internal transcribed spacer 2 (ITS2) secondary structures.Bioinformatics1116161623
- 9. Harpke D, Peterson A (2008a) 5.8S motifs for the identification of pseudogenic ITS regions. Botany 86: 300–305.D. HarpkeA. Peterson2008a5.8S motifs for the identification of pseudogenic ITS regions.Botany86300305
- 10. Harpke D, Peterson A (2008b) Extensive 5.8S nrDNA polymorphism in Mammillaria (Cactaceae) with special reference to the identification of pseudogenic internal transcribed spacer region. J Plant Res 121: 261–270.D. HarpkeA. Peterson2008bExtensive 5.8S nrDNA polymorphism in Mammillaria (Cactaceae) with special reference to the identification of pseudogenic internal transcribed spacer region.J Plant Res121261270
- 11. Ochieng JW, Henry RJ, Baverstock PR, Steane DA, Shepherd M (2007) Nuclear ribosomal pseudogenes resolve a corroborated monophyly of the eucalypt genus Corymbia despite misleading hypotheses at functional ITS paralogs. Mol Phylogenet Evol 44: 752–764.JW OchiengRJ HenryPR BaverstockDA SteaneM. Shepherd2007Nuclear ribosomal pseudogenes resolve a corroborated monophyly of the eucalypt genus Corymbia despite misleading hypotheses at functional ITS paralogs.Mol Phylogenet Evol44752764
- 12. Xiao L-Q, Möller M, Zhu H (2010) High nrDNA ITS polymorphism in the ancient extant seed plant Cycas: Incomplete concerted evolution and the origin of pseudogenes. Mol Phylogenet Evol 55: 168–177.L-Q XiaoM. MöllerH. Zhu2010High nrDNA ITS polymorphism in the ancient extant seed plant Cycas: Incomplete concerted evolution and the origin of pseudogenes.Mol Phylogenet Evol55168177
- 13. Dover GA, Strachan T, Coen ES (1982) Molecular drive. Science 218: 1069.GA DoverT. StrachanES Coen1982Molecular drive.Science2181069
- 14. Baldwin BG, Sanderson MJ, Porter MJ, Wojciechowski MF, Campbell CS, et al. (1995) The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann Mo Bot Gard 82: 247–277.BG BaldwinMJ SandersonMJ PorterMF WojciechowskiCS Campbell1995The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny.Ann Mo Bot Gard82247277
- 15. Elder JF Jr, Turner BJ (1995) Concerted evolution of repetitive DNA sequences in eukaryotes. Q Rev Biol 70: 297–320.JF Elder JrBJ Turner1995Concerted evolution of repetitive DNA sequences in eukaryotes.Q Rev Biol70297320
- 16. Wendel JF, Schnabel A, Seelanan T (1995) Bidirectional interlocus concerted evolution following allopolyploid speciation in cotton (Gossypium). Proc Natl Acad Sci U S A 92: 280–284.JF WendelA. SchnabelT. Seelanan1995Bidirectional interlocus concerted evolution following allopolyploid speciation in cotton (Gossypium).Proc Natl Acad Sci U S A92280284
- 17. Alvarez I, Wendel JF (2003) Ribosomal ITS sequences and plant phylogenetic inference. Mol Phylogenet Evol 29: 417–434.I. AlvarezJF Wendel2003Ribosomal ITS sequences and plant phylogenetic inference.Mol Phylogenet Evol29417434
- 18. Kovařík A, Matyášek R, Lim KY, Skalická K, Koukalová B, et al. (2004) Concerted evolution of 18-5.8-26S rDNA repeats in Nicotiana allotetraploids. Biol J Linn Soc 82: 615–625.A. KovaříkR. MatyášekKY LimK. SkalickáB. Koukalová2004Concerted evolution of 18-5.8-26S rDNA repeats in Nicotiana allotetraploids.Biol J Linn Soc82615625
- 19. Francisco-Ortega J, Barber JC, Santos-Guerra A, Febles-Hernández R, Jansen RR (2001) Origin and evolution of the endemic genera of Gonosperminae (Asteraceae: Anthemideae) from the Canary Islands: Evidence from nucleotide sequences of the internal transcribed spacers of the nuclear ribosomal DNA. Am J Bot 88: 161–169.J. Francisco-OrtegaJC BarberA. Santos-GuerraR. Febles-HernándezRR Jansen2001Origin and evolution of the endemic genera of Gonosperminae (Asteraceae: Anthemideae) from the Canary Islands: Evidence from nucleotide sequences of the internal transcribed spacers of the nuclear ribosomal DNA.Am J Bot88161169
- 20. Renner SS, Schaefer H, Kocyan A (2007) Phylogenetics of Cucumis (Cucurbitaceae): Cucumber (C. sativus) belongs in an Asian/Australian clade far from melon (C. melo). BMC Evol Biol 7: 58.SS RennerH. SchaeferA. Kocyan2007Phylogenetics of Cucumis (Cucurbitaceae): Cucumber (C. sativus) belongs in an Asian/Australian clade far from melon (C. melo).BMC Evol Biol758Available: http://www.biomedcentral.com/1471-2148/7/58. Accessed 2010 Aug 12. Available: http://www.biomedcentral.com/1471-2148/7/58. Accessed 2010 Aug 12.
- 21. Pettengill JB, Neel MC (2008) Phylogenetic patterns and conservation among North American members of the genus Agalinis (Orobanchaceae). BMC Evol Biol 8: 264.JB PettengillMC Neel2008Phylogenetic patterns and conservation among North American members of the genus Agalinis (Orobanchaceae).BMC Evol Biol8264Available: http://www.biomedcentral.com/1471-2148/8/264. Accessed 2010 Aug 12. Available: http://www.biomedcentral.com/1471-2148/8/264. Accessed 2010 Aug 12.
- 22. Sun K, Chen X, Ma R, Li C, Wang Q, et al. (2002) Molecular phylogenetics of Hippophae L. (Elaegnaceae) based on the internal transcribed spacer (ITS) sequence of nrDNA. Plant Syst Evol 235: 121–134.K. SunX. ChenR. MaC. LiQ. Wang2002Molecular phylogenetics of Hippophae L. (Elaegnaceae) based on the internal transcribed spacer (ITS) sequence of nrDNA.Plant Syst Evol235121134
- 23. Liu QL, Ge S, Tang HB, Zhang XL, Zhu GF, et al. (2006) Phylogenetic relationships in Elymus (Poaceae : Triticeae) based on the nuclear ribosomal internal transcribed spacer and chloroplast trnL-F sequences. New Phytol 170: 411–4206.QL LiuS. GeHB TangXL ZhangGF Zhu2006Phylogenetic relationships in Elymus (Poaceae : Triticeae) based on the nuclear ribosomal internal transcribed spacer and chloroplast trnL-F sequences.New Phytol1704114206
- 24. Wang QD, Zhang T, Wang JB (2007) Phylogenetic relationships and hybrid origin of Potamogeton species (Potamogetonaceae) distributed in China: insight from the nuclear ribosomal internal transcribed spacer sequence (ITS). Plant Syst Evol 267: 65–78.QD WangT. ZhangJB Wang2007Phylogenetic relationships and hybrid origin of Potamogeton species (Potamogetonaceae) distributed in China: insight from the nuclear ribosomal internal transcribed spacer sequence (ITS).Plant Syst Evol2676578
- 25. O'Kane SL, Schaal BA, Al-Shehbaz IA (1996) The origin of Arabidopsis suecica as indicated by nuclear rDNA sequences. Syst Bot 21: 559–566.SL O'KaneBA SchaalIA Al-Shehbaz1996The origin of Arabidopsis suecica as indicated by nuclear rDNA sequences.Syst Bot21559566
- 26. Popp M, Oxelman B (2001) Inferring the history of the polyploid Silene aegaea (Caryophyllacea) using plastid and homeologous nuclear DNA sequences. Mol Phylogenet Evol 20: 474–481.M. PoppB. Oxelman2001Inferring the history of the polyploid Silene aegaea (Caryophyllacea) using plastid and homeologous nuclear DNA sequences.Mol Phylogenet Evol20474481
- 27. Bennet RI, Smith AG (1991) Use of a genomic clone for ribosomal RNA from Brassica oleracea in RFLP analysis of Brassica species. Plant Mol Biol 16: 685–688.RI BennetAG Smith1991Use of a genomic clone for ribosomal RNA from Brassica oleracea in RFLP analysis of Brassica species.Plant Mol Biol16685688
- 28. Barkman TJ, Simpson BB (2002) Hybrid origin and parentage of Dendrochilum acuiferum (Orchidaceae) infered in a phylogenetic context using nuclear and plastid DNA sequence data. Syst Bot 27: 209–220.TJ BarkmanBB Simpson2002Hybrid origin and parentage of Dendrochilum acuiferum (Orchidaceae) infered in a phylogenetic context using nuclear and plastid DNA sequence data.Syst Bot27209220
- 29. Volkov RA, Komarova NY, Hemleben V (2007) Ribosomal DNA in plant hybrids: inheritance, rearrangement, expression. Syst Biodivers 5: 261–276.RA VolkovNY KomarovaV. Hemleben2007Ribosomal DNA in plant hybrids: inheritance, rearrangement, expression.Syst Biodivers5261276
- 30. Dadejová M, Lim KY, Součková-Skalická K, Matyášek R, Grandbastien M-A, et al. (2007) Transcription activity of rRNA genes correlates with a tendency towards intergenomic homogenization in Nicotiana allotetraploids. New Phytol 174: 658–668.M. DadejováKY LimK. Součková-SkalickáR. MatyášekM-A Grandbastien2007Transcription activity of rRNA genes correlates with a tendency towards intergenomic homogenization in Nicotiana allotetraploids.New Phytol174658668
- 31. Kovařík A, Pires JC, Leitch AR, Lim KY, Sherwood AM, et al. (2005) Rapid concerted evolution of nuclear ribosomal DNA in two Tragopogon allopolyploids of recent and recurrent origin. Genetics 169: 931–944.A. KovaříkJC PiresAR LeitchKY LimAM Sherwood2005Rapid concerted evolution of nuclear ribosomal DNA in two Tragopogon allopolyploids of recent and recurrent origin.Genetics169931944
- 32. Lim KY, Matyášek R, Kovařík A, Leitch A (2004) Genome evolution in allotetraploid Nicotiana. Biol J Linn Soc 82: 599–606.KY LimR. MatyášekA. KovaříkA. Leitch2004Genome evolution in allotetraploid Nicotiana.Biol J Linn Soc82599606
- 33. Volkov RA, Borisjuk NV, Panchuk II, Schweizer D, Hemleben V (1999) Elimination and rearrangement of parental rDNA in the allopolyploid Nicotiana tabacum. Mol Biol Evol 16: 311–320.RA VolkovNV BorisjukII PanchukD. SchweizerV. Hemleben1999Elimination and rearrangement of parental rDNA in the allopolyploid Nicotiana tabacum.Mol Biol Evol16311320
- 34. Mayol M, Rosselló JA (2001) Why nuclear ribosomal DNA spacers (ITS) tell us different stories in Quercus. Mol Phylogenet Evol 19: 167–176.M. MayolJA Rosselló2001Why nuclear ribosomal DNA spacers (ITS) tell us different stories in Quercus.Mol Phylogenet Evol19167176
- 35. Simmonds NW, Shepherd K (1955) The taxonomy and origins of the cultivated bananas. Bot J Linn Soc 55: 302–312.NW SimmondsK. Shepherd1955The taxonomy and origins of the cultivated bananas.Bot J Linn Soc55302312
- 36. Cheesman EE (1947) Classification of the bananas II. The genus Musa L. Kew Bull 2: 106–117.EE Cheesman1947Classification of the bananas II. The genus Musa L.Kew Bull2106117
- 37. Gawel NJ, Jarret RL, Whittemore AP (1992) Restriction fragment length polymorphism (RFLP)-based phylogenetic analysis of Musa. Theor Appl Genet 84: 286–290.NJ GawelRL JarretAP Whittemore1992Restriction fragment length polymorphism (RFLP)-based phylogenetic analysis of Musa.Theor Appl Genet84286290
- 38. Lanaud C, Tezenas du Montcel H, Jolivot MP, Glaszmann JC, González de León D (1992) Variation of ribosomal gene spacer length among wild and cultivated banana. Heredity 68: 147–156.C. LanaudH. Tezenas du MontcelMP JolivotJC GlaszmannD. González de León1992Variation of ribosomal gene spacer length among wild and cultivated banana.Heredity68147156
- 39. Wong C, Kiew R, Argent G, Set O, Lee SK, et al. (2002) Assessment of the validity of the section in Musa (Musaceae) using AFLP. Ann Bot 90: 231–238.C. WongR. KiewG. ArgentO. SetSK Lee2002Assessment of the validity of the section in Musa (Musaceae) using AFLP.Ann Bot90231238
- 40. Ude G, Pillay M, Ogundiwin E, Tenkouano A (2002) Analysis of genetic diversity and sectional relationships in Musa using AFLP markers. Theor Appl Genet 104: 1239–1245.G. UdeM. PillayE. OgundiwinA. Tenkouano2002Analysis of genetic diversity and sectional relationships in Musa using AFLP markers.Theor Appl Genet10412391245
- 41. Risterucci AM, Hippolyte I, Perrier X, Xia L, Caig V, et al. (2009) Development and assesment of Diversity Array technology for high-throughput DNA analysis in Musa. Theor Appl Genet 119: 1093–1103.AM RisterucciI. HippolyteX. PerrierL. XiaV. Caig2009Development and assesment of Diversity Array technology for high-throughput DNA analysis in Musa.Theor Appl Genet11910931103
- 42. Bartoš J, Alkhimova O, Doleželová M, De Langhe E, Doležel J (2005) Nuclear genome size and genomic distribution of ribosomal DNA in Musa and Ensete (Musaceae): taxonomic implications. Cytogenet Genome Res 109: 50–57.J. BartošO. AlkhimovaM. DoleželováE. De LangheJ. Doležel2005Nuclear genome size and genomic distribution of ribosomal DNA in Musa and Ensete (Musaceae): taxonomic implications.Cytogenet Genome Res1095057
- 43. Hakkinen M (2004) Musa voonii, a new Musa species from northern Borneo and discussion of the section Callimusa in Borneo. Acta Phytotaxon Geobot 55: 79–88.M. Hakkinen2004Musa voonii, a new Musa species from northern Borneo and discussion of the section Callimusa in Borneo.Acta Phytotaxon Geobot557988
- 44. Hakkinen M (2005) Musa azizii, a new Musa species (Musaceae) from northern Borneo. Acta Phytotaxon Geobot 56: 27–31.M. Hakkinen2005Musa azizii, a new Musa species (Musaceae) from northern Borneo.Acta Phytotaxon Geobot562731
- 45. Amorim EP, Vilarinhos AD, Cohen KO, Amorim VBO, dos Santos-Serejo JA, et al. (2009) Genetic diversity of carotenoid–rich bananas evaluated by Diversity Arrays Technology. Genet Mol Biol 32: 96–103.EP AmorimAD VilarinhosKO CohenVBO AmorimJA dos Santos-Serejo2009Genetic diversity of carotenoid–rich bananas evaluated by Diversity Arrays Technology.Genet Mol Biol3296103
- 46. Buhariwalla HK, Jarret RL, Jayashree B, Crouch JH, Ortiz R (2005) Isolation and characterization of microsatellite markers from Musa balbisiana. Mol Ecol Notes 5: 327–330.HK BuhariwallaRL JarretB. JayashreeJH CrouchR. Ortiz2005Isolation and characterization of microsatellite markers from Musa balbisiana.Mol Ecol Notes5327330
- 47. Wang XL, Chiang TY, Roux N, Hao G, Ge XJ (2007) Genetic diversity of wild banana (Musa balbisiana Colla) in China as revealed by AFLP markers. Genet Resour Crop Evol 54: 1125–1132.XL WangTY ChiangN. RouxG. HaoXJ Ge2007Genetic diversity of wild banana (Musa balbisiana Colla) in China as revealed by AFLP markers.Genet Resour Crop Evol5411251132
- 48. Nwakanma DC, Pillay M, Okoli BE, Tenkouano A (2003) PCR-RFLP of the ribosomal DNA internal transcribed spacer (ITS) provides markers for the A and B genomes in Musa L. Theor Appl Genet 108: 154–159.DC NwakanmaM. PillayBE OkoliA. Tenkouano2003PCR-RFLP of the ribosomal DNA internal transcribed spacer (ITS) provides markers for the A and B genomes in Musa L.Theor Appl Genet108154159
- 49. Li L-F, Hakkinen M, Yuan Y-M, Hao G, Ge X-J (2010) Molecular phylogeny and systematics of the banana family (Musaceae) inferred from multiple nuclear and chloroplast DNA fragments, with a special reference to the genus Musa. Mol Phylogenet Evol 57: 1–10.L-F LiM. HakkinenY-M YuanG. HaoX-J Ge2010Molecular phylogeny and systematics of the banana family (Musaceae) inferred from multiple nuclear and chloroplast DNA fragments, with a special reference to the genus Musa.Mol Phylogenet Evol57110
- 50. Liu A-Z, Kress J, Li D-Z (2010) Phylogenetic analyses of the banana family (Musaceae) based on nuclear ribosomal (ITS) and chloroplast (trnL-F) evidence. Taxon 59: 20–28.A-Z LiuJ. KressD-Z Li2010Phylogenetic analyses of the banana family (Musaceae) based on nuclear ribosomal (ITS) and chloroplast (trnL-F) evidence.Taxon592028
- 51. Christelová P, Valárik M, Høibová E, De Langhe E, Doležel J (2011) A multi-gene sequence based phylogeny of the Musaceae (banana) family. BMC Evol Biol (submitted). P. ChristelováM. ValárikE. HøibováE. De LangheJ. Doležel2011A multi-gene sequence based phylogeny of the Musaceae (banana) family.BMC Evol Biol (submitted)
- 52. Bailey CD, Carr TG, Harris SA, Hughes CE (2003) Characterization of angiosperm nrDNA polymorphism, paralogy and pseudogenes. Mol Phylogenet Evol 29: 435–455.CD BaileyTG CarrSA HarrisCE Hughes2003Characterization of angiosperm nrDNA polymorphism, paralogy and pseudogenes.Mol Phylogenet Evol29435455
- 53. Kim ST, Sultan SE, Donoghue MJ (2008) Allopolyploid speciation in Persicaria (Polygonaceae): insights from a low-copy nuclear region. Proc Natl Acad Sci U S A 105: 12370–12375.ST KimSE SultanMJ Donoghue2008Allopolyploid speciation in Persicaria (Polygonaceae): insights from a low-copy nuclear region.Proc Natl Acad Sci U S A1051237012375
- 54. Ritz CM, Schmuths H, Wissemann V (2005) Evolution by reticulation: European dogroses originated by multiple hybridization across the genus Rosa. J Hered 96: 4–14.CM RitzH. SchmuthsV. Wissemann2005Evolution by reticulation: European dogroses originated by multiple hybridization across the genus Rosa.J Hered96414
- 55. De Langhe E, Høibová E, Carpentier S, Doležel J, Swennen R (2010) Did backcrossing contribute to the origine of hybrid edible bananas? Ann Bot 106: 849–857.E. De LangheE. HøibováS. CarpentierJ. DoleželR. Swennen2010Did backcrossing contribute to the origine of hybrid edible bananas?Ann Bot106849857
- 56. Wissemann V (2002) Molecular evidence for allopolyploid origin of the Rosa canina complex (Rosaceae, Rosoideae). J Appl Bot 76: 176–178.V. Wissemann2002Molecular evidence for allopolyploid origin of the Rosa canina complex (Rosaceae, Rosoideae).J Appl Bot76176178
- 57. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, et al. (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 473: 376–380.M. MarguliesM. EgholmWE AltmanS. AttiyaJS Bader2005Genome sequencing in microfabricated high-density picolitre reactors.Nature473376380
- 58. Simmond NW (1962) The evolution of the bananas. London: Longmans. 170 p.NW Simmond1962The evolution of the bananasLondonLongmans170
- 59. Shepherd K (1988) Observation on Musa taxonomy. pp. 158–165.K. Shepherd1988Observation on Musa taxonomy.158165In: Identification of Genetic Diversity in the Genus Musa. Proc. Int. Workshop held at Los Banos, Philippines 5–10 September. INIBAP, Montpellier, France.
- 60. Simmond NW (1960) Notes on banana taxonomy. Kew Bull 14: 198–212.NW Simmond1960Notes on banana taxonomy.Kew Bull14198212
- 61. Samuel R, Bachmair A, Jobst J, Ehrendorfer F (1998) ITS sequences from nuclear rDNA suggest phylogenetic relationships between Euro-Mediterranean, East Asiatic and North American Quercus (Fagaceae). Plant Syst Evol 211: 129–139.R. SamuelA. BachmairJ. JobstF. Ehrendorfer1998ITS sequences from nuclear rDNA suggest phylogenetic relationships between Euro-Mediterranean, East Asiatic and North American Quercus (Fagaceae).Plant Syst Evol211129139
- 62. Manos P, Doyle JJ, Nixon KC (1999) Phylogeny, biogeography and processes of molecular differentiation in Quercus subgenus Quercus (Fagaceae). Mol Phylogenet Evol 12: 333–349.P. ManosJJ DoyleKC Nixon1999Phylogeny, biogeography and processes of molecular differentiation in Quercus subgenus Quercus (Fagaceae).Mol Phylogenet Evol12333349
- 63. Staden R (1996) The Staden sequence analysis package. Mol Biotechnol 5: 233–241.R. Staden1996The Staden sequence analysis package.Mol Biotechnol5233241
- 64. Zhang HB, Zhao X, Ding X, Paterson AH, Wing RA (1995) Preparation of megabase-size DNA from plant nuclei. Plant J 7: 175–184.HB ZhangX. ZhaoX. DingAH PatersonRA Wing1995Preparation of megabase-size DNA from plant nuclei.Plant J7175184
- 65. Šafář J, Noa-Carrazana JC, Vrána J, Bartoš J, Alkhimova O, et al. (2004) Creation of a BAC resource to study the structure and evolution of the banana (Musa balbisiana) genome. Genome 47: 1182–1191.J. ŠafářJC Noa-CarrazanaJ. VránaJ. BartošO. Alkhimova2004Creation of a BAC resource to study the structure and evolution of the banana (Musa balbisiana) genome.Genome4711821191
- 66. Meyer M, Briggs AW, Maricic T, Hober B, Hoffner B, et al. (2008) From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing. Nucl Acids Res 36: e5.M. MeyerAW BriggsT. MaricicB. HoberB. Hoffner2008From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing.Nucl Acids Res36e5
- 67. Smit AFA, Hubley R, Green P (1996–2004) RepeatMasker Open-3.0. AFA SmitR. HubleyP. Green1996–2004RepeatMasker Open-3.0.Available: http://www.repeatmasker.org. Available: http://www.repeatmasker.org.
- 68. Huan X, Madan A (1999) CAP3: A DNA sequence assembly program. Genome Res 9: 868–877.X. HuanA. Madan1999CAP3: A DNA sequence assembly program.Genome Res9868877
- 69. Macas J, Neumann P, Navrátilová A (2007) Repetitive DNA in the pea (Pisum sativum L.) genome: comprehensive characterization using 454 sequencing and comparison to soybean and Medicago truncatula. BMC Genomics 8: 427.J. MacasP. NeumannA. Navrátilová2007Repetitive DNA in the pea (Pisum sativum L.) genome: comprehensive characterization using 454 sequencing and comparison to soybean and Medicago truncatula.BMC Genomics8427Available: http://www.biomedcentral.com/1471-2164/8/427. Accessed 2010 Sep 10. Available: http://www.biomedcentral.com/1471-2164/8/427. Accessed 2010 Sep 10.
- 70. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792–1797.RC Edgar2004MUSCLE: multiple sequence alignment with high accuracy and high throughput.Nucleic Acids Res3217921797
- 71. Galtier N, Gouy M, Gautier C (1996) SeaView and Phylo_win, two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12: 543–548.N. GaltierM. GouyC. Gautier1996SeaView and Phylo_win, two graphic tools for sequence alignment and molecular phylogeny.Comput Appl Biosci12543548
- 72. Kumar S, Tamura K, Nei M (1994) MEGA, Molecular Evolutionary Genetics Analysis software for microcomputers. Bioinformatics 10: 189–191.S. KumarK. TamuraM. Nei1994MEGA, Molecular Evolutionary Genetics Analysis software for microcomputers.Bioinformatics10189191
- 73. Mathews DH, Sabina J, Zuker M, Turner DH (1999) Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure. J Mol Biol 288: 911–940.DH MathewsJ. SabinaM. ZukerDH Turner1999Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure.J Mol Biol288911940
- 74. Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31: 3406–3415.M. Zuker2003Mfold web server for nucleic acid folding and hybridization prediction.Nucleic Acids Res3134063415
- 75. Tomlinson PB (1962) Phylogeny of the Scitamineae: Morphological and anatomical considerations. Evolution 16: 192–213.PB Tomlinson1962Phylogeny of the Scitamineae: Morphological and anatomical considerations.Evolution16192213
- 76. Kress WJ, Prince LM, Hahn WJ, Zimmer EA (2001) Unraveling the evolution of the families of the Zingiberales using morphological and molecular evidence. Syst Biol 50: 926–944.WJ KressLM PrinceWJ HahnEA Zimmer2001Unraveling the evolution of the families of the Zingiberales using morphological and molecular evidence.Syst Biol50926944
- 77. Huson DH, Bryant D (2006) Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23: 254–267.DH HusonD. Bryant2006Application of phylogenetic networks in evolutionary studies.Mol Biol Evol23254267
- 78. Posada D (2008) jModelTest: Phylogenetic model averaging. Mol Biol Evol 25: 1253–1256.D. Posada2008jModelTest: Phylogenetic model averaging.Mol Biol Evol2512531256
- 79. Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52: 696–704.S. GuindonO. Gascuel2003A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood.Syst Biol52696704
- 80. Akaike H (1974) A new look at the statistical model identification. IEEE Trans Autom Control 19: 716–723.H. Akaike1974A new look at the statistical model identification.IEEE Trans Autom Control19716723
- 81. Drummond AJ, Rambaut A (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol 7: 214.AJ DrummondA. Rambaut2007BEAST: Bayesian evolutionary analysis by sampling trees.BMC Evol Biol7214Available: http://www.biomedcentral.com/1471-2148/7/214. Accessed 2010 Sep 10. Available: http://www.biomedcentral.com/1471-2148/7/214. Accessed 2010 Sep 10.
- 82. Wuyts J, De Rijk P, Van de Peer Y, Winkelmans T, De Wachter R (2001) The European large subunit ribosomal RNA database. Nucleic Acids Res 29: 175–177.J. WuytsP. De RijkY. Van de PeerT. WinkelmansR. De Wachter2001The European large subunit ribosomal RNA database.Nucleic Acids Res29175177
- 83. Perrier X, Bakry F, Carreel F, Jenny C, Horry J-P, et al. (2009) Combining biological approaches to shed light on the evolution of edible bananas. Ethnobotany Research & Applications 7: 199–216.X. PerrierF. BakryF. CarreelC. JennyJ-P Horry2009Combining biological approaches to shed light on the evolution of edible bananas.Ethnobotany Research & Applications7199216