Physiological costs of rapid growth may contribute to the observation that organisms typically grow at submaximal rates. Although, it has been hypothesized that faster growing individuals would do worse in dealing with suboptimal temperatures, this type of cost has never been explored empirically. Furthermore, the mechanistic basis of the physiological costs of rapid growth is largely unexplored.
Larvae of the damselfly Ischnura elegans from two univoltine northern and two multivoltine southern populations were reared at three temperatures and after emergence given a cold shock. Cold resistance, measured by chill coma recovery times in the adult stage, was lower in the southern populations. The faster larval growth rates in the southern populations contributed to this latitudinal pattern in cold resistance. In accordance with their assumed role in cold resistance, Hsp70 levels were lower in the southern populations, and faster growing larvae had lower Hsp70 levels. Yet, individual variation in Hsp70 levels did not explain variation in cold resistance.
We provide evidence for a novel cost of rapid growth: reduced cold resistance. Our results indicate that the reduced cold resistance in southern populations of animals that change voltinism along the latitudinal gradient may not entirely be explained by thermal selection per se but also by the costs of time constraint-induced higher growth rates. This also illustrates that stressors imposed in the larval stage may carry over and shape fitness in the adult stage and highlights the importance of physiological costs in the evolution of life-histories at macro-scales.
Citation: Stoks R, De Block M (2011) Rapid Growth Reduces Cold Resistance: Evidence from Latitudinal Variation in Growth Rate, Cold Resistance and Stress Proteins. PLoS ONE 6(2): e16935. https://doi.org/10.1371/journal.pone.0016935
Editor: Geoffrey Trussell, Northeastern University, United States of America
Received: November 4, 2010; Accepted: January 5, 2011; Published: February 24, 2011
Copyright: © 2011 Stoks, De Block. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research was supported by a research grant of the Fund for Scientific Research - Flanders (G.0419.08) and research grants from the KULeuven Research Fund (GOA/2008/06 and PF/10/007). M. De Block is postdoctoral researcher of the Fund for Scientific Research - Flanders. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Growth rate is a key life history trait that will determine age and size at maturity and therefore contributes in shaping adult fitness , . Despite the obvious benefits of growing fast, i.e. reaching a large size in a short time, it is becoming widely accepted that animals typically are not growing at their maximum speed –. This has initiated a search for counterbalancing costs of growing fast with most focus on ecological costs like increased predation risk (e.g. ref –).
Physiological costs of rapid growth, i.e. those entailing a lower ability to endure adverse environmental conditions, are much less studied . Most attention went to food shortage demonstrating that faster growing individuals did worse in coping with starvation (e.g. ref –). Although, it has been hypothesized that faster growing individuals would also do worse in dealing with suboptimal temperatures , this type of cost has never been explored empirically.
Costs as part of trade-offs can not only be studied at the individual but also at the population level because also interpopulation differences in growth rates exist. As the benefits and costs of rapid growth may vary among populations, optimal growth rates also show geographic variation . In this context, time constraints associated with the length of the available growth period play an important role in shaping higher growth rates . Changes in voltinism with more generations in low-latitude (-altitude) populations are widely documented along latitudinal (altitudinal) gradients –, and have been identified as a key factor causing higher time constraints and therefore higher growth rates in these populations –. This generates the hypothesis that the reduced ability to deal with low temperatures in low-latitude (-altitude) populations (e.g. ref – may be partly explained as a physiological cost of the higher growth rates rather than by thermal selection per se.
The mechanistic basis of the physiological costs of rapid growth is largely unexplored. With regard to cold resistance, stress proteins like Hsp70 seem to play an important role in insects. Several studies showed an upregulation of Hsp70 under cold stress (e.g. ref –) which improves survival at low temperatures –. Also, chill coma recovery times, a key measure of cold resistance, have been linked to PGI genotypes , and PGI genotypes differ in Hsp70 expression . Furthermore, higher Hsp70 levels are associated with reduced growth rates (overview in ). Altogether, this suggests that the proposed physiological cost of rapid growth in terms of reduced cold resistance may be mediated through reduced expression of Hsp70 in fast growers.
The overall aim of the present study is to evaluate physiological costs of rapid growth in terms of reduced cold resistance, as measured by chill coma recovery times, both at the individual and at the population level using the damselfly Ischnura elegans as a model system. At the population level we compared two northern univoltine with two southern multivoltine populations. Given the stronger time constraints associated with multivoltinism, we expected higher growth rates in the southern populations . Based on previous empirical research (see above) we expected lower cold resistance in the southern populations, and rapid growth to be associated with reduced cold resistance potentially through a link with lower Hsp70 levels. Because patterns in growth rate and cold resistance among populations along latitudinal gradients may depend on rearing temperature , , , we reared larvae at three temperatures from the egg stage in a common-garden experiment. Clear latitudinal patterns in growth rate were observed. Higher growth rates were associated with reduced cold tolerance both at the population and at the individual level, and this relationship was consistent with differences in Hsp70 levels at the population level.
Materials and Methods
The damselfly Ischnura elegans is a very abundant damselfly in Europe occurring from northern Spain to southern Sweden . In northern Europe it has one generation a year (univoltine), while in southern Europe it has multiple generations a year (multivoltine) . Two populations were studied from southern Sweden, Genarp (GE) and Vallby (VA), and two from southern France, St-Martin de Crau (SM) and Salin de Giraud (SG). To assess latitudinal differences in growth rate and cold resistance and their plastic responses to rearing temperature, we reared larvae of the four study populations from the egg stage until emergence at three temperatures (18°C, 21°C and 24°C) and measured chill coma recovery time one day after emergence in the adult stage. This temperature range has been shown to generate clear thermal reaction norms in related species  and spans the natural temperature regime of the populations of this species during the largest part of the growth season. Furthermore, survival is low when larvae are reared at lower and higher temperatures.
We collected 8–10 females for each of four study populations. Field-collected females were placed individually in small plastic containers and given wet filter paper as oviposition substrate and allowed to oviposit for three days in the laboratory. Afterwards they were released in the field. Rearing experiments were performed with the permission (09-06037) of the Flemish Agency of Nature and Forestry. Filter papers with eggs were transported to Belgium where they were kept at 21°C. At the day of hatching, larvae were randomly divided among six identical incubators set at 18°C, 21°C and 24°C (14∶10 L:D photoperiod). Each larva was placed individually in a circular plastic 180 ml cup filled to a height of 5 cm with aged dechlorinated tap water. Cups were rotated daily within the incubator and regularly between the incubators of the same temperature treatment. Larvae were daily fed ad libitum with brine shrimp nauplii. When larvae entered the final instar the daily food ration was doubled. We daily checked animals for adult emergence. Development time was calculated as the number of days between egg hatching and adult emergence. One day after emergence, each adult was weighed to the nearest 0.01 mg and randomly assigned to either a control or a cold shock treatment that induced a comatose condition. Larval growth rate was quantified as ln(mass at emergence)/development time (see e.g. ref –). This growth rate based on the entire larval period correlates strongly with growth rates based on mass increase during the final instar in this species (R. Stoks, unpublished data). For the life history variables, sample sizes per combination of latitude and temperature varied between 35 and 53 (total n = 261).
Cold resistance was measured as chill coma recovery time; an assay used successfully before to demonstrate intraspecific latitudinal and altitudinal patterns (e.g. ref –, –). For this, we placed individual one-day old adults in a microcentrifuge tube in an incubator at 4°C at 11am. We chose this chilling temperature based on the cold challenges imposed on the natural adult populations (File S1). After 1.5 h each adult was gently placed on its back in a petri dish with roughened bottom at 21°C. We scored recovery times to the nearest second as the time taken for an animal to stand upright. Following recovery, animals were given another hour to allow for the possible upregulation of Hsp70 and were then frozen at −80°C (see ref ). No animals died during the cold shock. For recovery times, sample size per combination of latitude and temperature varied between 21 and 26 animals (total n = 140).
We quantified Hsp70 levels using an immunoblot assay closely following the protocol of Slos and Stoks . Briefly, single larvae were homogenised in a proteinase inhibitor cocktail (Sigma®, St Louis, MO P2714) and a sample corresponding with 10 µg of protein was separated using SDS-polyacrylamide gel electrophoresis (PAGE). Therefore, any patterns in Hsp70 are independent of total protein content. Stress proteins were detected using monoclonal primary antibodies cross-reacting with the stress-induced Hsp70 and the constitutive Hsc70 (dilution 1∶1500, SPA 757, Stressgen®) and a AP-conjugated secondary antibody (dilution 1∶1000, D0486, DakoCytomation®, Glostrup, Denmark). The optical density (OD) of stress protein bands on the membrane was quantified on digitized images using the software package Image ProPlus. To correct for potential variation between blots, we ran a control sample of 1 µL HeLa Cell Lysate (Heat shocked; Stressgen®) on every blot. For Hsp70 the response curve for optical density against concentration is linear in damselflies . For Hsp70, we analyzed per combination of latitude and temperature 11–13 control animals that received no cold shock and 22–26 animals that received a cold shock (total n = 212). Control animals for Hsp70 analysis were similarly treated as the ones given a cold shock but placed 1.5 h in an incubator at 21°C and not at 4°C. We assayed more larvae after the cold shock as our focus was on testing for covariation of Hsp70 levels and recovery times.
We tested for effects of rearing temperature, latitude, and population nested in latitude on the dependent variables in separate general linear models. Population nested in latitude was included as a random factor; it was never significant (all P>0.23) indicating consistent results within a given latitude. In all analyses we also included sex and its interactions but these results are not related to out predictions and did not interfere with the observed patterns and therefore will not be reported. Models on life history (age and mass at emergence, growth rate) initially also included the cold shock treatment (present vs absent) to evaluate whether we successfully randomized larvae across the two adult cold shock treatments. Yet, it was never significant (all P>0.13) and not retained in the final models. The cold shock treatment was also included as a factor when analyzing Hsp70 levels but not when analyzing recovery times because for the latter variable all animals had been given a cold shock. For the analyses of recovery times (log-transformed) and Hsp70 levels we included mass as a covariate, and for the latter also the optical density of the Hela control. Correct degrees of freedom were estimated using the Satterthwaite option. Because the results on larval development time ( = age) and mass at emergence are not the focus of this paper they are presented in File S2 and Figure S1.
Southern larvae had much higher growth rates than northern larvae (F1,1.81 = 123.13, P = 0.011; Fig. 1A). With increasing temperature growth rate increased (F2,248 = 54.67, P<0.0001). This temperature-induced plasticity in growth rate was less pronounced in southern larvae (Latitude × Temperature, F2,248 = 5.66, P = 0.0039).
Mean (±1 SE) larval growth rate (A), and Hsp70 level (B) and chill coma recovery time (C) in the adult stage of Ischnura elegans from two northern and two southern populations at three rearing temperatures. Means are slightly offset to aid visualization. Hsp70 levels and chill coma recovery times are quantified after a cold shock treatment (1.5 h exposure to 4°C) given to the freshly emerged adults.
After a cold shock, Hsp70 levels were slightly lower (F1,186 = 5.59, P = 0.019; mean OD ±1SE, without cold shock: 20,800±320, with cold shock: 20,100±210). Hsp70 levels were lower in southern animals (F1,186 = 4.28, P = 0.040) and especially the rearing temperature effect was strong with considerably lower Hsp70 levels at the higher temperature (F2,186 = 25.80, P<0.0001) (Fig. 1B). When adding growth rate to the model the latitude (F1,20.9 = 7.84, P = 0.011) and temperature (F2,180 = 5.80, P = 0.0036) effects remained; larvae with a higher growth rate had lower Hsp70 levels (F1,178 = 23.90, P = 0.040, R2 = 0.09; Fig. 2A).
Relationships between larval growth rate and residual model values of (A) Hsp70 level and (B) chill coma recovery time in the adult stage of Ischnura elegans. Residuals were derived from the general models described in the methods and therefore independent of temperature and latitude.
Recovery times were longer in southern animals (F1,127 = 8.27, P = 0.0047) and in animals reared at higher temperatures (F2,127 = 38.64, P<0.0001) (Fig. 1C). When also including larval growth rate and Hsp70 levels in the model, the latitudinal effect was no longer significant (F1,10.6 = 1.03, P = 0.33) while the temperature effect remained significant (F2,124 = 10.97, P<0.0001). Larvae with a higher growth rate had longer recovery times (F1,123 = 9.71, P = 0.0023, R2 = 0.06; Fig. 2B). Hsp70 levels did not affect recovery times (F1,124 = 0.63, P = 0.43).
In line with the higher perceived time constraints –, –, , growth rates were higher in the southern multivoltine than in the northern univoltine populations. Similar latitudinal growth rate patterns associated with changes in voltinism have been documented in butterflies , mosquitoes , and in a previous study on I. elegans (Shama et al., unpublished data). We also found the typical increase in growth rates at higher temperatures . This increase was less pronounced in the southern populations where growth rates were already high at the low temperature, suggesting growth rates were near their physiological maximum .
We provide evidence of a novel cost of rapid growth: rapid growth was associated with a reduced cold resistance both at the latitudinal level and at the individual level. Importantly, these patterns cannot simply result from differences in mass, as all analyses were mass-corrected.
More generally, our results support the untested hypothesis by Gotthard  that faster growing individuals would be worse in dealing with suboptimal temperatures. In ectotherms like damselflies that forage and mate in flight, there are obvious fitness implications of a higher cold resistance in the adult stage. Adults with a better cold resistance would better endure cold nights and likely be active earlier in the day, hence can spend more time foraging and engaging in reproductive activities . This cost may be general in animals and plants, yet not directly considered in previous studies. As in our study, southern populations of the pitcher plant mosquito have higher growth rates  and a lower cold resistance than northern populations . Further, lowland populations of the copper butterfly have higher growth rates and reduced cold resistance . Finally, faster growing plant species have higher frost damage .
Cold resistance, as measured by shorter chill coma recovery times, was higher in the northern populations and higher at the lowest rearing temperature. This is in line with studies along latitudinal gradients in other insects (e.g. ref , –), which considered this latitudinal pattern as a direct result of geographic differences in thermal selection. Also in our study system thermal selection for increased cold resistance is likely higher in the northern populations (File S1). Yet, the observation that latitudinal differences in cold resistance were not significant anymore when growth rate was added to the model indicates growth rate differences are contributing to the latitudinal differences in cold resistance. We therefore hypothesize that the latitudinal pattern in cold resistance in our study system may not be entirely explained by thermal selection per se but also by the higher growth rates at lower latitudes.
While there was some indication that the physiological cost of rapid growth in terms of reduced cold resistance was mediated through reduced Hsp70 levels there was no support for this at the individual level. Treatment groups with higher growth rates and longer recovery times, i.e. southern populations and animals reared at higher temperatures, indeed had lower Hsp70 levels, and at the individual level faster growing animals had lower Hsp70 levels. Yet, individual variation in Hsp70 levels did not explain variation in recovery times when taking into account the treatment effects. A reason for this may be that we could not detect an upregulation of Hsp70 under cold stress and that we therefore obtained baseline Hsp70 levels (see below). More general, other (stress) proteins may also play a role in shaping the still poorly understood resistance to nonfreezing low temperatures –, potentially obscuring any effect, if present, from Hsp70 alone.
Instead of an upregulation of Hsp70 after the cold shock, we found a slight downregulation. In the copper butterfly Karl et al.  did report an upregulation of Hsp70 after a 1 h cold stress followed by a 1 h recovery period. Yet, in line with our results, other studies found that (mRNA) levels of Hsp70, if anything, decreased during a cold period and only started increasing several hours after the cold shock ended , , . Activation of the heat shock factors is probably incomplete under a short period of cold stress, and longer recovery times would have shown an upregulation , . Whatever the reason, this lack of upregulation during the experiment indicates that baseline Hsp levels (i.e. those present before the cold shock) may have contributed in shaping the latitudinal differences in recovery times in current experiment. Upregulation of Hsp70 after cold stress may be more important in reducing damage long after the chill coma has ended as shown in another insect  and may not be that important in keeping chill coma recovery times short. Similarly, Koštál and Tollarová-Borovanská  suggested that high Hsp levels during dormancy may represent an anticipatory protection against a variety of environmental insults.
Hsp70 levels were lower in the two treatment groups with the highest growth rates, southern larvae and larvae reared at the high temperature, consistent with an energetic cost of rapid growth . Importantly, also at the individual level faster growing larvae had lower Hsp70 levels. Note that, development time (nor body mass) did not covary with Hsp70 levels when also growth rate was included in the model, so it is not a faster life history (or associated patterns in body mass) per se that seems to shape Hsp70 patterns. This trade-off pattern between growth rate and Hsp70 levels adds to the few other studies showing that higher Hsp levels were associated with lower growth rates (overview in ). For example, Drosophila larvae with extra copies of the Hsp70 gene have decreased growth rates compared to control larvae . This trade-off is thought to be energy-mediated as the synthesis, functioning and maintenance of Hsp proteins is energetically costly .
To conclude, we here presented evidence for a, likely widespread, novel cost of rapid growth in terms of reduced cold resistance. Our study thereby is complementary to the few other studies demonstrating physiological costs of rapid growth in terms of reduced resistance against food stress (see introduction), oxidative stress – and reduced immune function – and offers a new explanation why organisms typically not grow at their maximal rates –. Noteworthy, this type of cost would never have been detected when only focusing on the adult stage, stressing the importance to consider both life stages when trying to understand life history variation in animals with a complex life cycle . This adds to the insight that stressors imposed in the larval stage may carry over and shape fitness in the adult stage –. Furthermore, this type of cost may also contribute to the here documented latitudinal patterns in cold resistance. Changes in voltinism with more generations in low-latitude (-altitude) populations are widely documented –, and have been identified as a key factor generating higher time constraints and therefore higher growth rates in these populations –. Together with current findings this may indicate that the reduced ability to deal with low temperatures in low-latitude (-altitude) populations of animals that change voltinism along these gradients (e.g. ref –) may not be entirely explained by thermal selection per se but also by the costs of the time constraint-induced higher growth rates. Our results thereby highlight the importance of physiological costs in the evolution of life-histories at macro-scales. Given that cold resistance is a key factor shaping range limits, similar studies at the interplay between physiological ecology and macro-ecology may prove rewarding in understanding range boundaries and their shifts under global warming –.
Effects of latitude and temperature on age and mass at emergence.
Differences in development time and mass at emergence between latitudes across temperatures. Mean (±1 SE) larval development time (A), and mass at emergence (B) of Ischnura elegans from the two northern and two southern populations at the three rearing temperatures. Means are slightly offset to aid visualization.
We are grateful to Patrick Grillas and Ine Swillen for help with egg collection and the Swedish Meteorological Institute (SMHI) and Philippe Chauvelon (Tour-du-Valat) for providing temperature data.
Conceived and designed the experiments: RS MDB. Performed the experiments: RS MDB. Analyzed the data: RS. Contributed reagents/materials/analysis tools: RS MDB. Wrote the paper: RS MDB.
- 1. Roff DA (1992) The evolution of life histories. New York: Chapman and Hall. 536 p.DA Roff1992The evolution of life histories.New YorkChapman and Hall536
- 2. Stearns S (1992) Evolution of life histories. Oxford: Oxford University Press. 249 p.S. Stearns1992Evolution of life histories.OxfordOxford University Press249
- 3. Arendt JD (1997) Adaptive intrinsic growth rates: an integration across taxa. Q Rev Biol 72: 149–173.JD Arendt1997Adaptive intrinsic growth rates: an integration across taxa.Q Rev Biol72149173
- 4. Nylin S, Gottard K (1998) Plasticity in life-history traits. Annu Rev Entomol 43: 63–83.S. NylinK. Gottard1998Plasticity in life-history traits.Annu Rev Entomol436383
- 5. Tammaru T, Nylin S, Ruohomaki K, Gotthard K (2004) Compensatory responses in lepidopteran larvae: a test of growth rate maximization. Oikos 107: 352–362.T. TammaruS. NylinK. RuohomakiK. Gotthard2004Compensatory responses in lepidopteran larvae: a test of growth rate maximization.Oikos107352362
- 6. Gotthard K (2000) Increased risk of predation as a cost of high growth rate: an experimental test in a butterfly. J Anim Ecol 69: 896–902.K. Gotthard2000Increased risk of predation as a cost of high growth rate: an experimental test in a butterfly.J Anim Ecol69896902
- 7. Stoks R, De Block M, Van de Meutter F, Johansson F (2005) Predation cost of rapid growth: behavioural coupling and physiological decoupling. J Anim Ecol 74: 708–715.R. StoksM. De BlockF. Van de MeutterF. Johansson2005Predation cost of rapid growth: behavioural coupling and physiological decoupling.J Anim Ecol74708715
- 8. Gotthard K (2001) Growth strategies of ectothermic animals in temperate environments. In: Atkinson D, Thorndyke M, editors. Environment and animal development: Genes, life histories and plasticity. Oxford: BIOS. pp. 287–303.K. Gotthard2001Growth strategies of ectothermic animals in temperate environments.D. AtkinsonM. ThorndykeEnvironment and animal development: Genes, life histories and plasticityOxfordBIOS287303
- 9. Gotthard K, Nylin S, Wiklund C (1994) Adaptive variation in growth rate: life history costs and consequences in the speckled wood butterfly, Pararge aegeria. Oecologia 99: 281–289.K. GotthardS. NylinC. Wiklund1994Adaptive variation in growth rate: life history costs and consequences in the speckled wood butterfly, Pararge aegeria.Oecologia99281289
- 10. Fischer K, Zeilstra I, Hetz SK, Fiedler K (2004) Physiological costs of growing fast: does accelerated growth reduce pay-off in adult fitness? Evol Ecol 18: 343–353.K. FischerI. ZeilstraSK HetzK. Fiedler2004Physiological costs of growing fast: does accelerated growth reduce pay-off in adult fitness?Evol Ecol18343353
- 11. Stoks R, De Block M, McPeek MA (2006) Physiological costs of compensatory growth in a damselfly. Ecology 87: 1566–1574.R. StoksM. De BlockMA McPeek2006Physiological costs of compensatory growth in a damselfly.Ecology8715661574
- 12. Abrams PA, Leimar O, Nylin S, Wiklund C (1996) The effect of flexible growth rates on optimal sizes and development times in a seasonal environment. Am Nat 147: 381–395.PA AbramsO. LeimarS. NylinC. Wiklund1996The effect of flexible growth rates on optimal sizes and development times in a seasonal environment.Am Nat147381395
- 13. Corbet PS, Suhling F, Söndgerath D (2006) Voltinism of Odonata: a review. Int J Odonatol 9: 1–44.PS CorbetF. SuhlingD. Söndgerath2006Voltinism of Odonata: a review.Int J Odonatol9144
- 14. Altermatt F (2010) Climatic warming increases voltinism in European butterflies and moths. Proc Roy Soc B 277: 1281–1287.F. Altermatt2010Climatic warming increases voltinism in European butterflies and moths.Proc Roy Soc B27712811287
- 15. Ragland GJ, Kingsolver JG (2007) Influence of seasonal timing on thermal ecology and thermal reaction norm evolution in Wyeomyia smithii. J Evol Biol 20: 2144–2153.GJ RaglandJG Kingsolver2007Influence of seasonal timing on thermal ecology and thermal reaction norm evolution in Wyeomyia smithii.J Evol Biol2021442153
- 16. Karl I, Janowitz SA, Fischer K (2008) Altitudinal life-history variation and thermal adaptation in the copper butterfly Lycaenus tityrus. Oikos 117: 778–788.I. KarlSA JanowitzK. Fischer2008Altitudinal life-history variation and thermal adaptation in the copper butterfly Lycaenus tityrus.Oikos117778788
- 17. Ragland GJ, Kingsolver JG (2008) Evolution of thermotolerance in seasonal environments: The effects of annual temperature variation and life-history timing in Wyeomyia smithii. Evolution 62: 1345–1357.GJ RaglandJG Kingsolver2008Evolution of thermotolerance in seasonal environments: The effects of annual temperature variation and life-history timing in Wyeomyia smithii.Evolution6213451357
- 18. Karl I, Sorensen JG, Loeschcke V, Fischer K (2009) HSP70 expression in the Copper butterfly Lycaena tityrus across altitudes and temperatures. J Evol Biol 22: 172–178.I. KarlJG SorensenV. LoeschckeK. Fischer2009HSP70 expression in the Copper butterfly Lycaena tityrus across altitudes and temperatures.J Evol Biol22172178
- 19. Colinet H, Lee SF, Hoffmann AA (2010) Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster. FEBS Journal 277: 174–185.H. ColinetSF LeeAA Hoffmann2010Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster.FEBS Journal277174185
- 20. Rinehart JP, Li A, Yocum GD, Robich RM, Hayward SA, et al. (2007) Up-regulation of heat shock proteins is essential for cold survival during insect diapauses. Proc Natl Acad Sci USA 104: 11130–11137.JP RinehartA. LiGD YocumRM RobichSA Hayward2007Up-regulation of heat shock proteins is essential for cold survival during insect diapauses.Proc Natl Acad Sci USA1041113011137
- 21. Koštál V, Tollarová-Borovanská M (2009) The 70 kDa heat shock protein assists during the repair of chilling injury in the insect Pyrrhocoris apterus. PLoS ONE 4: e4546.V. KoštálM. Tollarová-Borovanská2009The 70 kDa heat shock protein assists during the repair of chilling injury in the insect Pyrrhocoris apterus.PLoS ONE4e4546
- 22. Karl I, Schmitt T, Fischer K (2008) Phosphoglucose isomerase genotype affects life-history traits and cold stress resistance in a Copper butterfly. Funct Ecol 22: 887–894.I. KarlT. SchmittK. Fischer2008Phosphoglucose isomerase genotype affects life-history traits and cold stress resistance in a Copper butterfly.Funct Ecol22887894
- 23. Dahlhoff EP, Rank NE (2000) Functional and physiological consequences of genetic variation at phosphoglucose isomerase: Heat shock protein expression is related to enzyme genotype in a montane beetle. Proc Natl Acad Sci USA 97: 10056–10061.EP DahlhoffNE Rank2000Functional and physiological consequences of genetic variation at phosphoglucose isomerase: Heat shock protein expression is related to enzyme genotype in a montane beetle.Proc Natl Acad Sci USA971005610061
- 24. Sørensen JG, Kristensen TN, Loeschcke V (2003) The evolutionary and ecological role of heat shock proteins. Ecol Lett 6: 1025–1037.JG SørensenTN KristensenV. Loeschcke2003The evolutionary and ecological role of heat shock proteins.Ecol Lett610251037
- 25. Ayres MP, Scriber JM (1994) Local adaptation to regional climates in Papilio canadensis (Lepidoptera, Papilionidae). Ecol Monogr 64: 465–482.MP AyresJM Scriber1994Local adaptation to regional climates in Papilio canadensis (Lepidoptera, Papilionidae).Ecol Monogr64465482
- 26. Dijkstra KDB, Lewington R (2006) Field guide to the dragonflies of Britain and Europe. Dorset: British Wildlife Publishing. 320p p.KDB DijkstraR. Lewington2006Field guide to the dragonflies of Britain and Europe.DorsetBritish Wildlife Publishing320p
- 27. Van Doorslaer W, Stoks R (2005) Growth rate plasticity to temperature in two damselfly species differing in latitude: contributions of behaviour and physiology. Oikos 111: 599–605.W. Van DoorslaerR. Stoks2005Growth rate plasticity to temperature in two damselfly species differing in latitude: contributions of behaviour and physiology.Oikos111599605
- 28. Johansson F, Rowe L (1999) Life history and behavioural responses to time constraints in a damselfly. Ecology 80: 1242–1252.F. JohanssonL. Rowe1999Life history and behavioural responses to time constraints in a damselfly.Ecology8012421252
- 29. Johansson F, Stoks R, Rowe L, De Block M (2001) Life history plasticity in a damselfly: effects of combined time and biotic constraints. Ecology 82: 1857–1869.F. JohanssonR. StoksL. RoweM. De Block2001Life history plasticity in a damselfly: effects of combined time and biotic constraints.Ecology8218571869
- 30. Richter-Boix A, Teplitsky C, Rogell B, Laurila A (2010) Local selection modifies phenotypic divergence among Rana temporaria populations in the presence of gene flow. Mol Ecol 19: 716–731.A. Richter-BoixC. TeplitskyB. RogellA. Laurila2010Local selection modifies phenotypic divergence among Rana temporaria populations in the presence of gene flow.Mol Ecol19716731
- 31. Hoffmann AA, Anderson A, Hallas R (2002) Opposing clines for high and low temperature resistance in Drosophila melanogaster. Ecol Lett 5: 614–618.AA HoffmannA. AndersonR. Hallas2002Opposing clines for high and low temperature resistance in Drosophila melanogaster.Ecol Lett5614618
- 32. Hoffmann AA, Shirriffs J, Scott M (2005) Relative importance of plastic vs genetic factors in adaptive differentiation: geographical variation for stress resistance in Drosophila melanogaster from eastern Australia. Funct Ecol 19: 222–227.AA HoffmannJ. ShirriffsM. Scott2005Relative importance of plastic vs genetic factors in adaptive differentiation: geographical variation for stress resistance in Drosophila melanogaster from eastern Australia.Funct Ecol19222227
- 33. Slos S, Stoks R (2008) Predation risk induces stress proteins and reduces antioxidant defense. Funct Ecol 22: 637–642.S. SlosR. Stoks2008Predation risk induces stress proteins and reduces antioxidant defense.Funct Ecol22637642
- 34. De Block M, Stoks R (2004) Life-history variation in relation to time constraints in a damselfly. Oecologia 140: 68–75.M. De BlockR. Stoks2004Life-history variation in relation to time constraints in a damselfly.Oecologia1406875
- 35. Nygren GH, Bergström A, Nylin S (2008) Latitudinal body size clines in the butterfly Polyommatus icarus are shaped by gene-environment interactions. J Ins Science 8: 1–13.GH NygrenA. BergströmS. Nylin2008Latitudinal body size clines in the butterfly Polyommatus icarus are shaped by gene-environment interactions.J Ins Science8113
- 36. Angiletta MJ (2009) Thermal adaptation: a theoretical and empirical synthesis. Oxford: Oxford University Press. 289p p.MJ Angiletta2009Thermal adaptation: a theoretical and empirical synthesis.OxfordOxford University Press289p
- 37. Corbet PS (1999) Dragonflies, behaviour and ecology of Odonata. Essex: Harley Books. 829p p.PS Corbet1999Dragonflies, behaviour and ecology of Odonata.EssexHarley Books829p
- 38. Turnbulll LA, Paul-Victor C, Schmid B, Purves DW (2008) Growth rates, seed size, and physiology: Do small-seeded species really grow faster? Ecology 89: 1352–1363.LA TurnbulllC. Paul-VictorB. SchmidDW Purves2008Growth rates, seed size, and physiology: Do small-seeded species really grow faster?Ecology8913521363
- 39. Clower KJ, Lyman RF, Mackay TFC, Morgan TJ (2010) Genetic variation in senescence marker protein-30 is associated with natural variation in cold tolerance in Drosophila. Gen Res 92: 103–113.KJ ClowerRF LymanTFC MackayTJ Morgan2010Genetic variation in senescence marker protein-30 is associated with natural variation in cold tolerance in Drosophila.Gen Res92103113
- 40. Colinet H, Lee SF, Hoffmann AA (2010) Functional characterization of the Frost gene in Drosophila melanogaster: importance for recovery from chill coma. PLoS ONE 5: e10925.H. ColinetSF LeeAA Hoffmann2010Functional characterization of the Frost gene in Drosophila melanogaster: importance for recovery from chill coma.PLoS ONE5e10925
- 41. Sonoda S, Fukumoto K, Izumi Y, Yoshida H, Tsumuki H (2006) Cloning of heat shock protein genes (hsp90 and hsc70) and their expression during larval diapause and cold tolerance acquisition in the rice stem borer, Chilo suppressalis Walker. Arch Ins Biochem Physiol 63: 36–47.S. SonodaK. FukumotoY. IzumiH. YoshidaH. Tsumuki2006Cloning of heat shock protein genes (hsp90 and hsc70) and their expression during larval diapause and cold tolerance acquisition in the rice stem borer, Chilo suppressalis Walker.Arch Ins Biochem Physiol633647
- 42. Krebs RA, Feder ME (1997) Deleterious consequences of Hsp70 overexpression in Drosophila melanogaster larvae. Cell stress & Chap 2: 60–71.RA KrebsME Feder1997Deleterious consequences of Hsp70 overexpression in Drosophila melanogaster larvae.Cell stress & Chap26071
- 43. De Block M, Stoks R (2008) Compensatory growth and oxidative stress in a damselfly. Proc Roy Soc B 275: 781–785.M. De BlockR. Stoks2008Compensatory growth and oxidative stress in a damselfly.Proc Roy Soc B275781785
- 44. Hall ME, Blount JD, Forbes S, Royle NJ (2010) Does oxidative stress mediate the trade-off between growth and self-maintenance in structured families? Funct Ecol 24: 365–373.ME HallJD BlountS. ForbesNJ Royle2010Does oxidative stress mediate the trade-off between growth and self-maintenance in structured families?Funct Ecol24365373
- 45. Soler JJ, de Neve L, Perez-Contreras T, Soler M, Sorci G (2003) Trade-off between immunocompetence and growth in magpies: an experimental study. Proc Roy Soc B 270: 241–248.JJ SolerL. de NeveT. Perez-ContrerasM. SolerG. Sorci2003Trade-off between immunocompetence and growth in magpies: an experimental study.Proc Roy Soc B270241248
- 46. De Block M, Stoks R (2008) Short-term larval food stress and associated compensatory growth reduce adult immune function in a damselfly. Ecol Entomol 33: 796–801.M. De BlockR. Stoks2008Short-term larval food stress and associated compensatory growth reduce adult immune function in a damselfly.Ecol Entomol33796801
- 47. Pechenik JA (2006) Larval experience and latent effects – metamorphosis is not a new beginning. Integr Comp Biol 46: 323–333.JA Pechenik2006Larval experience and latent effects – metamorphosis is not a new beginning.Integr Comp Biol46323333
- 48. Rolff J, Van de Meutter F, Stoks R (2004) Time constraints decouple age and size at maturity and physiological traits. Am Nat 164: 559–565.J. RolffF. Van de MeutterR. Stoks2004Time constraints decouple age and size at maturity and physiological traits.Am Nat164559565
- 49. De Block M, Stoks R (2005) Fitness effects from egg to reproduction: bridging the life history transition. Ecology 86: 185–197.M. De BlockR. Stoks2005Fitness effects from egg to reproduction: bridging the life history transition.Ecology86185197
- 50. Chown SL, Gaston KJ (1999) Exploring links between physiology and ecology at macro-scales: the role of respiratory metabolism in insects. Biol Rev 74: 87–120.SL ChownKJ Gaston1999Exploring links between physiology and ecology at macro-scales: the role of respiratory metabolism in insects.Biol Rev7487120
- 51. Gaston KJ, Chown SL, Calosi P, Bernardo J, Bilton DT, et al. (2009) Macrophysiology: a conceptual reunification. Am Nat 174: 595–612.KJ GastonSL ChownP. CalosiJ. BernardoDT Bilton2009Macrophysiology: a conceptual reunification.Am Nat174595612