Lysozymes are antimicrobial enzymes that perform a critical role in resisting infection in a wide-range of eukaryotes. However, using the nematode Caenorhabditis elegans as a model host we now demonstrate that deletion of the protist type lysozyme LYS-7 renders animals susceptible to killing by the fatal fungal human pathogen Cryptococcus neoformans, but, remarkably, enhances tolerance to the enteric bacteria Salmonella Typhimurium. This trade-off in immunological susceptibility in C. elegans is further mediated by the reciprocal activity of lys-7 and the tyrosine kinase abl-1. Together this implies a greater complexity in C. elegans innate immune function than previously thought.
Citation: Marsh EK, van den Berg MCW, May RC (2011) A Two-Gene Balance Regulates Salmonella Typhimurium Tolerance in the Nematode Caenorhabditis elegans. PLoS ONE 6(3): e16839. https://doi.org/10.1371/journal.pone.0016839
Editor: Matt Kaeberlein, University of Washington, United States of America
Received: November 26, 2010; Accepted: January 3, 2011; Published: March 2, 2011
Copyright: © 2011 Marsh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by a Medical Research Council (MRC) studentship award to EKM and by a Biotechnology and Biological Sciences Research Council (BBSRC) grant BB/F000138/1. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: RCM is an associate editor for both PLoS One and PLoS Pathogens.
The nematode Caenorhabditis elegans is now firmly established as a powerful model system for the study of host-pathogen interactions , , . Although many aspects of innate immunity are shared with higher vertebrates , nematodes lack a cell-mediated immune system and thus rely upon secreted antimicrobial molecules for a systemic immune response to pathogenic challenge .
One such group of antimicrobial enzymes, lysozymes, are evolutionarily ancient proteins that are actively lytic against a range of microbes . Whereas many organisms have only one or two lysozyme genes, C. elegans has a family of at least ten differentially regulated genes that are predicted to show significant functional diversity , . Within the C. elegans lysozyme family, lys-7 has been the most extensively studied. Expression of this molecule is strongly induced upon exposure of the animal to the pathogenic bacteria Serratia marcescens , Microbacterium nematophilum  and Salmonella Typhimurium . Furthermore, lys-7 knockout animals show enhanced susceptibility to M. nematophilum, indicating that lys-7 has a protective function against this pathogen .
Here we examine the function of LYS-7 during C. elegans infection with a number of human pathogens. Surprisingly, we show that LYS-7 acts together with the tyrosine kinase ABL-1 to regulate an immunological balance in which resistance to C. neoformans comes at the cost of susceptibility to S. Typhimurium. Since this phenotype is independent of infectious burden or disease persistence, our data suggest a novel function of LYS-7 in regulating pathogen tolerance in C. elegans.
lys-7 mutant animals are hyper-susceptible to killing by Cryptococcus neoformans
We, and others, have previously shown that the fatal fungal human pathogen Cryptococcus neoformans kills C. elegans , . A preliminary gene expression study of two C. elegans strains, daf-2(e1370) and fem-1(hc17)IV, that are intrinsically resistant to killing by the fungus revealed that lys-7 expression was strongly and constitutively induced in these animals (R. C. May, unpublished data). We therefore hypothesised that LYS-7 may play an important role in mediating resistance of the worm towards C. neoformans. In line with this prediction, a lys-7 knockout strain (ok1384) showed wild type brood size and longevity under non-infectious conditions (Figs S1A and B; Methods S1), but severely reduced survival following exposure to the fungus (Fig 1A).
lys-7 knockout animals show reduced survival relative to N2 upon infection with C. neoformans (A); p<0.0001, [lys-7(ok1384) n = 106; N2 n = 151], but wild type sensitivity to S. aureus (B) and P. aeruginosa (C), p>0.05, [lys-7(ok1384) n = 131; N2 n = 168] and p>0.2, [lys-7(ok1384) n = 104; N2 n = 111] respectively. Surprisingly, however, lys-7 mutant animals exhibit enhanced resistance to S. Typhimurium (D); p<0.0001, [lys-7(ok1384) n = 191; N2 n = 242].
lys-7 mutant animals are resistant to killing by Salmonella Typhimurium
In order to establish whether the loss of LYS-7 influenced immunity to other pathogens, we exposed lys-7 knockout animals to three organisms previously shown to be pathogenic towards C. elegans; the Gram-positive bacterium Staphylococcus aureus  and two Gram-negative bacteria Pseudomonas aeruginosa , ,  and Salmonella enterica serovar Typhimurium , . The susceptibility of lys-7 knockout animals to S. aureus and P. aeruginosa was indistinguishable from that of the wild type strain (Figs 1B and C), suggesting that the loss of LYS-7 does not damage the worm immune response to all pathogens. In marked contrast, however, lys-7 knockout animals are strongly resistant to killing by S. Typhimurium (Fig 1D). Identical effects were seen with a second, independent, allele: lys-7(ok1385) (Figs S1C and D), and an additional S. Typhimurium strain 14028 s (Fig S1E), indicating that this phenomenon, which we refer to as “balanced immunity”, is not allele or strain specific.
Resistance to S. Typhimurium is mediated by genetic compensation
We considered that the loss of lys-7 may trigger compensatory up-regulation of other genes that result in the observed resistance to S. Typhimurium. Expression analysis identified only four genes that are significantly up-regulated in both lys-7 knockout strains (ok1384 and ok1385) in comparison to wild type animals: abl-1 (2.65±0.94 fold up-regulation), fat-5 (1.82±0.15 fold up-regulation), clec-60 (1.59±0.08 fold up-regulation), and rga-6 (1.92±0.089 fold up-regulation).
We obtained knockout strains for each of these genes and tested their susceptibility to killing by C. neoformans and S. Typhimurium (Figs 2A and B). Remarkably, animals lacking either abl-1 or rga-6 were hypersensitive to C. neoformans but resistant to S. Typhimurium infection; a survival pattern which phenocopies that of the lys-7 knockout animals. fat-5 knockout animals show slight resistance to S. Typhimurium but a normal susceptibility to C. neoformans, whilst clec-60 mutant animals are indistinguishable from wild type animals in their survival following exposure to either pathogen.
Knockout C. elegans strains for the four genes which were identified by a microarray to be up-regulated in the lys-7 mutant background were assayed for survival upon infection with both C. neoformans and S. Typhimurium. The survival of abl-1(ok171) and rga-6(ok1316) animals was significantly lower than wild type animals upon C. neoformans infection (A), p<0.0001 and p<0.01 respectively. The remaining animals were unchanged from wild type survival, p>0.2, [N2 n = 186; abl-1(ok171) n = 130; rga-6(ok1316) n = 192; fat-5(ok460) n = 100; clec-60(tm2319) n = 110]. abl-1(ok171), rga-6(ok1316) and fat-5(tm420), but not clec-60(tm2319), animals exhibited enhanced resistance to S. Typhimurium (B), p<0.0001 in each case, [N2 n = 170; abl-1(ok171) n = 222; rga-6(ok1316) n = 100; fat-5(ok460) n = 162; clec-60(tm2319) n = 125]. (C) abl-1 mutant animals up-regulate lys-7 expression (average induction of 6.8 fold; p<0.05), whereas lys-7 expression is unchanged from wild type in the remaining mutant backgrounds. Data represent the mean expression of three independent experiments, ± S.E.M.
We next used qRT-PCR to assess whether lys-7 expression was altered in these knockout animals under normal culture conditions. lys-7 expression remained unchanged in fat-5, clec-60 and rga-6 knockout animals, but, interestingly, was significantly up-regulated in abl-1 mutants (Fig 2C). Thus lys-7 and abl-1 show reciprocal regulation of expression and identical loss-of-function phenotypes upon infectious challenge.
Importantly, the abl-1 phenotype is not allele specific (Figs S2A and B) nor do abl-1 animals show reduced lifespan, brood size or resistance to S. aureus infection (Figs S2C, D and E), indicating a specific role for ABL-1 in mediating immunological tolerance to S. Typhimurium.
Reciprocal expression of lys-7 and abl-1 is required for tolerance to S. Typhimurium
To investigate this compensatory mechanism further, we generated a series of double mutants for lys-7 and the three candidate genes (abl-1, fat-5 and rga-6) identified by resistance analysis, and tested their susceptibility to infection with C. neoformans and S. Typhimurium. Whilst lys-7(ok1384);abl-1(ok171) double mutants remained sensitive to killing by C. neoformans, the resistance to S. Typhimurium exhibited by both single mutants was completely abolished (Figs 3A and B). In contrast however, the other double mutants (rga-6(ok1316);lys-7(ok1384) and fat-5(ok460);lys-7(ok1384)) remained sensitive to killing by C. neoformans and resistant to killing by S. Typhimurium (Figs 3C and D), suggesting that the loss of lys-7 is dominant in these animals, although we note that the degree of resistance has been slightly reduced in the case of rga-6. Taken together, these data indicate that abl-1 and lys-7 act together to regulate S. Typhimurium resistance in C. elegans.
lys-7(ok1384)abl-1(ok171) double mutants remain hypersensitive to C. neoformans (A), similar to their corresponding single mutants; p<0.0001, [abl-1(ok171)lys-7(ok1384) n = 133; N2 n = 150], but the resistance exhibited by the single mutants to S. Typhimurium infection (B) has been completely suppressed; p>0.2, [abl-1(ok171)lys-7(ok1384) n = 110; N2 n = 94]. Double mutants between lys-7 and fat-5 or lys-7 and rga-6 were hypersensitive to C. neoformans infection (C), p<0.0001 in both cases, [N2 n = 207; lys-7(ok1384)fat-5(tm420) n = 100; lys-7(ok1384)rga-6(ok1316) n = 100]. Similarly, the resistance to S. Typhimurium exhibited by the lys-7 knockout strain was either unaltered (fat-5) or only slightly reduced (rga-6) by these secondary mutations (D), [N2 n = 170; lys-7(ok1384)fat-5(tm420) n = 100; lys-7(ok1384)rga-6(ok1316) n = 100].
S. Typhimurium burden in the mutant animals is unchanged from wild type
We questioned whether the resistance of lys-7(ok1384) and abl-1(ok171) mutant animals to S. Typhimurium was due to a lower bacterial load within infected animals. To test this, we exposed wild type, lys-7 and abl-1 animals to S. Typhimurium L1019, a GFP-expressing derivative of SL1344 (kindly provided by Jessica Blair and Laura Piddock, University of Birmingham) and quantified infectious burden over time both through viable counts (Fig 4A) and microscopy (Fig S3). We found no consistent difference between any of the strains.
(A) Bacterial load was assessed through viable counts. There was no difference between any of the strains, p>0.2, although there is a slight disparity between abl-1(ok171) and lys-7(ok1384) on day 4 of the infection, p<0.05, [abl-1(ok171) n = 60, lys-7(ok1384) n = 60; N2 n = 60]. (B) lys-7(ok1384) and abl-1(ok171) single mutants are tolerant to S. Typhimurium infection following a six-hour exposure, p<0.01 and p<0.0001 respectively, [lys-7(ok1384) n = 90; abl-1(ok171) n = 86; N2 n = 96].
We also considered the possibility that lys-7 and abl-1 knockout animals may be better able to limit S. Typhimurium proliferation within the gut. To test this, we restricted the animals' exposure to the pathogen to just six hours and then shifted them to the normal feeding bacteria E. coli OP50 (a regime previously shown to lead to persistent colonisation ). Under these conditions both lys-7 and abl-1 animals retain their strong tolerance phenotypes (Fig 4B), but we observed no difference in S. Typhimurium colonisation between the mutants and wild type animals microscopically (Fig S4). Thus the enhanced survival of lys-7 and abl-1 knockout animals is due to an increased tolerance of S. Typhimurium within the gut, rather than resistance to infection or limitation of bacterial growth.
The lysozyme LYS-7 has been well-described in C. elegans as an essential antimicrobial molecule , , , . Here we show that LYS-7 protects animals against C. neoformans-mediated killing, a function that is presumably attributable to the secondary chitinase (anti-fungal) activity exhibited by most lysozymes . Remarkably, however, lys-7 acts as a susceptibility factor for S. Typhimurium killing, as the loss of lys-7 more than doubles the median survival of Salmonella-challenged animals.
In a manner similar to the lys-7 mutant animals, we find that abl-1 mutant worms are hypersusceptible to killing by C. neoformans. Since these animals show up-regulation of lys-7, a gene that we demonstrate to be important for cryptococcal resistance, we therefore conclude that ABL-1 regulates immunity at two levels (by the up-regulation of lys-7 and by a second, lys-7 independent pathway), both of which are required for wild type resistance to C. neoformans.
In vertebrates this phenomenon of immunological trade-off, whereby resistance to one class of pathogens comes at the cost of increased susceptibility to others, has been well documented , , . Recently, analogous balance phenotypes have been described in Drosophila , , , , . However, our finding of a susceptibility trade-off in C. elegans, mediated by the activity of LYS-7 and the tyrosine kinase ABL-1, is the first report of such a phenotype in nematodes and, as such, has significant implications for our understanding of the evolution of innate immunity in animals.
Materials and Methods
C. elegans and pathogen strains used
C. elegans infection assays
20 µl of an overnight bacterial/fungal culture was inoculated onto 6 cm NGM plates, supplemented with the appropriate antibiotic if required, and lawns were allowed to grow at room temperature for 12 h. 15–30 animals at the L4 stage were picked onto each plate and subsequently transferred to newly seeded plates every 1–2 days. Survival was monitored every 24 hr and death was determined as a failure to respond to mechanical stimulus.
C. elegans bacterial CFU analysis
Infection load was assessed through viable counts. The assay was modified from ; L4 animals were infected with S. Typhimurium strain L1019. At each timepoint, ten replicates of six animals each were incubated in 200 µl M9 buffer containing 25 mM levamisole hydrochloride (Sigma) and ampicillin (1 mg/ml) for one hour. The ampicillin was subsequently removed by three washes of 200 µl M9 with 25 mM levamisole hydrochloride. Animals were lysed in this buffer for 10 s using the Precellys 24 Lysis and Homogeniser. Lysates were serially diluted in M9 and plated onto LB plates containing kanamycin (30 µg/ml) to select for L1019. Colonies were counted by eye and scaled to the original concentration per nematode.
S. Typhimurium persistence analysis
L4 animals were exposed to S. Typhimurium SL1344 on plates for six hours before being washed 3 times in M9 solution and shifted onto NGM plates seeded with 20 µl OP50 at 25°C. Animals were transferred to newly seeded plates every 1–2 days and scored for death (failure to respond to mechanical stimulus) every 1–2 days.
RNA isolation, cDNA preparation and qRT-PCR
Total RNA was extracted from each strain on three independent occasions. Full plates of staged L4 animals were homogenised in 400 µl lysis buffer (Qiagen) using the Precellys 24 Lysis and Homogeniser. RNA was isolated from these lysates using the “RNeasy Mini Kit” (Qiagen) according to the manufacturer's instructions. RNA samples were treated with “DNA-free” (Ambion, Inc.) and subsequently quantified using the Nanodrop ND1000 microspectrophotometer (NanoDrop Technologies, Inc.). cDNA was synthesised using SuperScript II (Invitrogen) with random primers (Promega) in a SensoQuest Labcycler, assuming a 1∶1 conversion. The absence of genomic DNA was confirmed by PCR of these cDNA products (34 cycles: 94°C, 25 s; 55°C, 30 s; 72°C, 60 s), the amplimers of which were separated by electrophoresis on a 2.5% agarose gel. Quantitative real-time PCR (qRT-PCR) was performed on each cDNA sample in triplicate using 2x SensiMix (dU) SYBR Green kit (Quantace), but with a reaction volume of 25 µl. In each reaction MgCl2 had a final concentration of 3 mM, primers (sequences can be found in Table S2) were used at 20 mM and a template concentration of 2.5 ng was used. Each plate was run on an ABI Prism 7000 instrument with the following thermal cycling conditions: 37°C, 10 min; 95°C, 10 min; 95°C, 15 s; 55°C, 30 s; 72°C, 30 s; steps 3–5 were repeated for 40 cycles.
All survival replicates were checked for consistency prior to being combined into single survival curves in Microsoft Excel using a macro-based template to calculate Kaplan-Meier survival probabilities every 24 hours. Each curve represents at least three independent experiments. Differences in survival were tested with a non-parametric log-rank analysis and assessed for significance using Chi squared. P-values below 0.05, after correcting for multiplicity, were taken to be significant. The data in the survival curves are presented as the proportion of animals surviving. The bacterial CFU analysis was carried out in Microsoft Excel and subsequently tested for significance using the Students' T-test (2 tailed, equal variance). The qRT-PCR was normalised to gpd-3 expression using the Comparative Ct method in Microsoft Excel , , and tested for statistical significance using the Students' T-test (2 tailed, equal variance, paired).
lys-7 mutant nematodes do not differ from wild type animals in lifespan or brood size and the resistance phenotype is maintained with alternative Salmonella and C. elegans strains. lys-7 knockout animals have a normal lifespan in non-infectious conditions (A); p>0.2, [lys-7(ok1384) n = 129; N2 n = 199], and are unimpaired in their ability to produce young (B), data represent mean ± s.d. A further independent lys-7 knockout strain (ok1385) showed the same balance phenotype, being hypersensitive to C. neoformans (C), p<0.001, [lys-7(ok1385) n = 101; N2 as Figure 1A] and resistant to S. Typhimurium SL1344 (D), p<0.0001, [lys-7(ok1385) n = 169; N2 as Figure 1D]. Moreover, the lys-7(ok1384) mutant worms exhibited the same enhanced resistance to infection with S. Typhimurium 14028 s (E) in comparison to the wild type strain; p<0.01, [lys-7(ok1385) n = 123; N2 n = 120].
abl-1 mutant nematodes do not show a reduction in lifespan or brood size and the resistance phenotype is maintained with alternative Salmonella and C. elegans strains. Two further independent abl-1 knockout strains showed the same balance phenotype, being hypersensitive to C. neoformans (A), p<0.0001 in both cases, [abl-1(n1961) n = 150; abl-1(n1963) n = 152; N2 n = 325] and resistant to S. Typhimurium SL1344 (B), p<0.0001 in both cases, [abl-1(n1961) n = 150; abl-1(n1963) n = 127; N2 n = 278]. abl-1 knockout animals have a normal lifespan under non-infectious conditions (C); p>0.2, [abl-1(ok171) n = 142; N2 n = 159], and are unimpaired in their ability to produce young (D), data represent mean ± s.d. Further, no difference between abl-1(ok171) and wild type survival upon S. aureus infection was detected (E), p>0.2, [abl-1(ok171) n = 120; N2 as Figure 1B]. Moreover, the abl-1 mutant worms exhibited the same enhanced resistance to infection with S. Typhimurium 14028 s (E) in comparison to the wild type strain; p<0.001, [abl-1(ok171) n = 90; N2 n = 112].
Microscopic analysis of a constant S. Typhimurium infection in C. elegans. Bacterial load of each C. elegans strain was assessed microscopically using a GFP-expressing S. Typhimurium, strain L1019, across a five day infection. No difference in infection load was identified between the strains.
Microscopic analysis of a six hour S. Typhimurium infection in C. elegans. Bacterial load of each C. elegans strain was assessed microscopically using a GFP-expressing S. Typhimurium, strain L1019, across a five day infection. No difference in infection load was identified between the strains.
Bacterial and fungal strains and their respective growth requirements.
We thank LJV Piddock, JM Blair and SA Johnston for their valued input throughout the project, and acknowledge NJ Davis, P Velica and C Pearce for their help with the expression studies. The nematode strains were provided by the Caenorhabditis Genetics Center, and the Horvitz and Mitani Laboratories.
Conceived and designed the experiments: EKM RCM. Performed the experiments: EKM MCWVDB. Analyzed the data: EKM RCM. Wrote the paper: EKM RCM.
- 1. Sifri CD, Begun J, Ausubel FM (2005) The worm has turned - microbial virulence modeled in Caenorhabditis elegans. Trends in Microbiology 13: 119–127.CD SifriJ. BegunFM Ausubel2005The worm has turned - microbial virulence modeled in Caenorhabditis elegans.Trends in Microbiology13119127
- 2. Schulenburg H, Kurz CL, Ewbank JJ (2004) Evolution of the innate immune system: the worm perspective. Immunological Reviews 198: 36–58.H. SchulenburgCL KurzJJ Ewbank2004Evolution of the innate immune system: the worm perspective.Immunological Reviews1983658
- 3. Ewbank JJ (2002) Tackling both sides of the host-pathogen equation with Caenorhabditis elegans. Microbes and Infection 4: 247–256.JJ Ewbank2002Tackling both sides of the host-pathogen equation with Caenorhabditis elegans.Microbes and Infection4247256
- 4. Ewbank JJ (2006) Signalling in the immune response. WormBook. JJ Ewbank2006Signalling in the immune response.WormBookIn: Community TCeR, ed.
- 5. Bachali S, Jager M, Hassanin A, Schoentgen F, Jolles P, et al. (2002) Phylogenetic analysis of invertebrate lysozymes and the evolution of lysozyme function. Journal of Molecular Evolution 54: 652–664.S. BachaliM. JagerA. HassaninF. SchoentgenP. Jolles2002Phylogenetic analysis of invertebrate lysozymes and the evolution of lysozyme function.Journal of Molecular Evolution54652664
- 6. Thomas JH (2006) Analysis of homologous gene clusters in Caenorhabditis elegans reveals striking regional cluster domains. Genetics 172: 127–143.JH Thomas2006Analysis of homologous gene clusters in Caenorhabditis elegans reveals striking regional cluster domains.Genetics172127143
- 7. Schulenburg H, Boehnisch C (2008) Diversification and adaptive sequence evolution of Caenorhabditis lysozymes (Nematoda: Rhabditidae). Bmc Evolutionary Biology 8: H. SchulenburgC. Boehnisch2008Diversification and adaptive sequence evolution of Caenorhabditis lysozymes (Nematoda: Rhabditidae).Bmc Evolutionary Biology8
- 8. Mallo GV, Kurz CL, Couillault C, Pujol N, Granjeaud S, et al. (2002) Inducible antibacterial defense system in C-elegans. Current Biology 12: 1209–1214.GV MalloCL KurzC. CouillaultN. PujolS. Granjeaud2002Inducible antibacterial defense system in C-elegans.Current Biology1212091214
- 9. O'Rourke D, Baban D, Demidova M, Mott R, Hodgkin J (2006) Genomic clusters, putative pathogen recognition molecules, and antimicrobial genes are induced by infection of C. elegans with M. nematophilum. Genome Research 16: 1005–1016.D. O'RourkeD. BabanM. DemidovaR. MottJ. Hodgkin2006Genomic clusters, putative pathogen recognition molecules, and antimicrobial genes are induced by infection of C. elegans with M. nematophilum.Genome Research1610051016
- 10. Evans EA, Kawli T, Tan MW (2008) Pseudomonas aeruginosa Suppresses Host Immunity by Activating the DAF-2 Insulin-Like Signaling Pathway in Caenorhabditis elegans. Plos Pathogens 4: EA EvansT. KawliMW Tan2008Pseudomonas aeruginosa Suppresses Host Immunity by Activating the DAF-2 Insulin-Like Signaling Pathway in Caenorhabditis elegans.Plos Pathogens4
- 11. Mylonakis E, Ausubel FM, Perfect JR, Heitman J, Calderwood SB (2002) Killing of Caenorhabditis elegans by Cryptococcus neoformans as a model of yeast pathogenesis. Proceedings of the National Academy of Sciences of the United States of America 99: 15675–15680.E. MylonakisFM AusubelJR PerfectJ. HeitmanSB Calderwood2002Killing of Caenorhabditis elegans by Cryptococcus neoformans as a model of yeast pathogenesis.Proceedings of the National Academy of Sciences of the United States of America991567515680
- 12. van den Berg MCW, Woerlee JZ, Ma HS, May RC (2006) Sex-dependent resistance to the pathogenic fungus Cryptococcus neoformans. Genetics 173: 677–683.MCW van den BergJZ WoerleeHS MaRC May2006Sex-dependent resistance to the pathogenic fungus Cryptococcus neoformans.Genetics173677683
- 13. Sifri CD, Begun J, Ausubel FM, Calderwood SB (2003) Caenorhabditis elegans as a model host for Staphylococcus aureus pathogenesis. Infection and Immunity 71: 2208–2217.CD SifriJ. BegunFM AusubelSB Calderwood2003Caenorhabditis elegans as a model host for Staphylococcus aureus pathogenesis.Infection and Immunity7122082217
- 14. Mahajan-Miklos S, Tan MW, Rahme LG, Ausubel FM (1999) Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans pathogenesis model. Cell 96: 47–56.S. Mahajan-MiklosMW TanLG RahmeFM Ausubel1999Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans pathogenesis model.Cell964756
- 15. Tan MW, Mahajan-Miklos S, Ausubel FM (1999) Killing of Caenorhabditis elegans by Pseudomonas aeruginosa used to model mammalian bacterial pathogenesis. Proceedings of the National Academy of Sciences of the United States of America 96: 715–720.MW TanS. Mahajan-MiklosFM Ausubel1999Killing of Caenorhabditis elegans by Pseudomonas aeruginosa used to model mammalian bacterial pathogenesis.Proceedings of the National Academy of Sciences of the United States of America96715720
- 16. Darby C, Cosma CL, Thomas JH, Manoil C (1999) Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences of the United States of America 96: 15202–15207.C. DarbyCL CosmaJH ThomasC. Manoil1999Lethal paralysis of Caenorhabditis elegans by Pseudomonas aeruginosa.Proceedings of the National Academy of Sciences of the United States of America961520215207
- 17. Labrousse A, Chauvet S, Couillault C, Kurz CL, Ewbank JJ (2000) Caenorhabditis elegans is a model host for Salmonella typhimurium. Current Biology 10: 1543–1545.A. LabrousseS. ChauvetC. CouillaultCL KurzJJ Ewbank2000Caenorhabditis elegans is a model host for Salmonella typhimurium.Current Biology1015431545
- 18. Aballay A, Yorgey P, Ausubel FM (2000) Salmonella typhimurium proliferates and establishes a persistent infection in the intestine of Caenorhabditis elegans. Current Biology 10: 1539–1542.A. AballayP. YorgeyFM Ausubel2000Salmonella typhimurium proliferates and establishes a persistent infection in the intestine of Caenorhabditis elegans.Current Biology1015391542
- 19. Jolles P, editor. (1996) Lysozymes: Model Enzymes in Biochemistry and Biology. Birkhauser. P. Jolles1996Lysozymes: Model Enzymes in Biochemistry and BiologyBirkhauser
- 20. Kim LK, Choi UY, Cho HS, Lee JS, Lee W-B, et al. (2007) Down-regulation of NF-KB target genes by the AP-1 and STAT complex during the innate immune response in Drosophila. PLoS Biology 5: LK KimUY ChoiHS ChoJS LeeW-B Lee2007Down-regulation of NF-KB target genes by the AP-1 and STAT complex during the innate immune response in Drosophila.PLoS Biology5
- 21. Nicholson LB, Kuchroo VK (1996) Manipulation of the Th1/Th2 balance in autoimmune disease. Current Opinion in Immunology 8: 837–842.LB NicholsonVK Kuchroo1996Manipulation of the Th1/Th2 balance in autoimmune disease.Current Opinion in Immunology8837842
- 22. Koguchi Y, Kawakami K (2002) Cryptococcal infection and the Th1-Th2 cytokine balance. International Reviews of Immunology 21: 423–438.Y. KoguchiK. Kawakami2002Cryptococcal infection and the Th1-Th2 cytokine balance.International Reviews of Immunology21423438
- 23. Ayres JS, Schneider DS (2008) A signaling protease required for melanization in Drosophila affects resistance and tolerance of infections. PLoS Biology 6: e305.JS AyresDS Schneider2008A signaling protease required for melanization in Drosophila affects resistance and tolerance of infections.PLoS Biology6e305
- 24. Schneider DS, Ayres JS, Brandt SM, Costa A, Dionne MS, et al. (2007) Drosophila eiger Mutants Are Sensitive to Extracellular Pathogens. PLoS Biology 3: e41.DS SchneiderJS AyresSM BrandtA. CostaMS Dionne2007Drosophila eiger Mutants Are Sensitive to Extracellular Pathogens.PLoS Biology3e41
- 25. Brandt SM, Dionne MS, Khush RS, Pham LN, Vigdal TJ, et al. (2004) Secreted Bacterial Effectors and Host-Produced Eiger/TNF Drive Death in a Salmonella-Infected Fruit Fly. PLoS Biology 2: e418.SM BrandtMS DionneRS KhushLN PhamTJ Vigdal2004Secreted Bacterial Effectors and Host-Produced Eiger/TNF Drive Death in a Salmonella-Infected Fruit Fly.PLoS Biology2e418
- 26. Gordon MD, Ayres JS, Schneider DS, Nusse R (2008) Pathogenesis of Listeria-Infected Drosphila wntD Mutants Is Associated with Elevated Levels of the Novel Immunity Gene edin. Plos Pathogens 4: e1000111.MD GordonJS AyresDS SchneiderR. Nusse2008Pathogenesis of Listeria-Infected Drosphila wntD Mutants Is Associated with Elevated Levels of the Novel Immunity Gene edin.Plos Pathogens4e1000111
- 27. Lazzaro BP, Sackton TB, Clark AG (2006) Genetic Variation in Drosophila melanogaster Resistance to Infection: A Comparison Across Bacteria. Genetics 174: 1539–1554.BP LazzaroTB SacktonAG Clark2006Genetic Variation in Drosophila melanogaster Resistance to Infection: A Comparison Across Bacteria.Genetics17415391554
- 28. Brenner S (1974) GENETICS OF CAENORHABDITIS-ELEGANS. Genetics 77: 71–94.S. Brenner1974GENETICS OF CAENORHABDITIS-ELEGANS.Genetics777194
- 29. Hope IA, editor. (1999) C. elegans: a practical approach: Oxford University Press. IA Hope1999C. elegans: a practical approach: Oxford University Press.
- 30. Kawli T, Tan MW (2008) Neuroendocrine signals modulate the innate immunity of Caenorhabditis elegans through insulin signaling. Nature Immunology 9: 1415–1424.T. KawliMW Tan2008Neuroendocrine signals modulate the innate immunity of Caenorhabditis elegans through insulin signaling.Nature Immunology914151424
- 31. Bookout AL, Manglesdorf DJ (2003) Quantitative real-time PCR protocol for analysis of nuclear receptor signaling pathways. Nuclear Receptor Signaling. e012 p.AL BookoutDJ Manglesdorf2003Quantitative real-time PCR protocol for analysis of nuclear receptor signaling pathways.Nuclear Receptor Signalinge012
- 32. Amrit FRG, Boehnisch CML, May RC (2010) Phenotypic Covariance of Longevity, Immunity and Stress Resistance in the Caenorhabditis Nematodes. PLoS One 4: e9978.FRG AmritCML BoehnischRC May2010Phenotypic Covariance of Longevity, Immunity and Stress Resistance in the Caenorhabditis Nematodes.PLoS One4e9978