We established a novel method, Gene Expression Profiling via Multigene Concatemers (MgC-GEP), to study multigene expression patterns simultaneously. This method consists of the following steps: (1) cDNA was obtained using specific reverse primers containing an adaptor. (2) During the initial 1–3 cycles of polymerase chain reaction (PCR), the products containing universal adaptors with digestion sites at both termini were amplified using specific forward and reverse primers containing the adaptors. (3) In the subsequent 4–28 cycles, the universal adaptors were used as primers to yield products. (4) The products were digested and ligated to produce concatemers. (5) The concatemers were cloned into the vector and sequenced. Then, the occurrence of each gene tag was determined. To validate MgC-GEP, we analyzed 20 genes in Saccharomyces cerevisiae induced by weak acid using MgC-GEP combined with real-time reverse transcription (RT)-PCR. Compared with the results of real-time RT-PCR and the previous reports of microarray analysis, MgC-GEP can precisely determine the transcript levels of multigenes simultaneously. Importantly, MgC-GEP is a cost effective strategy that can be widely used in most laboratories without specific equipment. MgC-GEP is a potentially powerful tool for multigene expression profiling, particularly for moderate-throughput analysis.
Citation: Jin K, Zheng X, Xia Y (2011) Gene Expression Profiling via Multigene Concatemers. PLoS ONE 6(1): e15711. https://doi.org/10.1371/journal.pone.0015711
Editor: Gisela Nogales-Gadea, University Hospital Vall d'Hebron, Spain
Received: July 28, 2010; Accepted: November 23, 2010; Published: January 18, 2011
Copyright: © 2011 Jin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants from the Project Foundation of Chongqing Municipal Education Committee (KJ08A04) and the Natural Science Foundation of China (30971913). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
With the completion of many genome projects , , , , , , , , research on addressing the roles of multiple genes in orchestrating complex cellular functions has attracted considerable attention. This requires the use of techniques that allow high-throughput analysis of such target genes. The analysis of multigene expression profiling is helpful to elucidate a series of medical and biological research questions including the dissection of basic biological processes , , the exploration of new drug targets , , , and the diagnosis of disease , , , . Thus, the research of multigene expression profiling has gradually become a key point in searching specific genes or proteins.
Several methods including DNA microarray , massive parallel signature sequencing (MPSS) , serial analysis of gene expression (SAGE) , SuperSAGE, RNA-seq and real-time multiplex reverse transcription (RT)-PCR  have been developed and widely applied in high-throughput multigene expression profiling , , , , , , . However, sometimes analyzing throughput of the gene transcription profiles is moderate in number. For example, one signal transduction pathway is often composed of between 10 and 50 genes , . A specific phenotype is often related to many gene families, which share substantial conservation at the protein and nucleotide level. Furthermore, gene expression in various samples often need be determined according to time course or different environmental stimuli. In these cases, using the methods mentioned above is not a sensible choice. For DNA microarray, though it could obtain comprehensive global expression survey, the information about some specific genes is not easily gleaned from these vast amounts of data produced , . Moreover, some homologous genes can cross-hybridize, which makes it difficult to determine specific genes of highly homologous gene family members. For MPSS, the new tool available for conducting multigene expression profiling , the specific equipments necessary restrict its application widely. For SAGE and SuperSAGE, those transcripts without the NlaIII or EcoP15I site may be missed which accounts for a few percent of the total transcripts in a given RNA sample , . For RNA-seq, several informatics challenges should be considered. The efficient methods to store, retrieve and process large amounts of data must be improved to reduce errors in image analysis and base-calling and remove low-quality reads . For real-time multiplex RT-PCR, it is not easy to optimize the identical reaction parameters of multiplex target amplification to obtain accurate quantification results , . Recently, the GenomeLab™ GeXP Genetic Analysis System was developed by Beckman & Coulter (http://www.beckman.com/products/instrument/geneticanalysis/gexp_inst_dcr.asp) combined RT-PCR and capillary electrophoresis (CE). This method is more suitable for characterizing the profiling of a moderate number of genes (10–50 genes) , . However, specific and expensive equipments are required, which also restricted its wide application. Therefore, it is necessary to establish an economical and simple method to analyze the transcript levels of dozens of genes in a particular pathway or related to a specific phenotype.
In this study, we developed a simple and reliable method, Gene Expression Profiling via Multigene Concatemers (MgC-GEP), for multigene expression profiling. We characterized the gene transcription of 20 genes in Saccharomyces cerevisiae that was under the weak acid stress using MgC-GEP and validated the results by real time RT-PCR. Our results show that MgC-GEP is a powerful gene expression profiling tool for a moderate number of multigenes to search and identify key players in the particular pathways of interest.
Materials and Methods
Strains and Vectors
S. cerevisiae strain W303-1A was obtained from CICIM-CU in Jiangnan University of China. The pUC19 T-vector (TaKaRa, China) was used to clone the concatemers. Escherichia coli strain JM109 was used for routine bacterial transformations and maintenance of plasmids.
Samples were prepared as previously described . Yeast cells from overnight cultures in yeast peptone dextrose (YPD) medium were diluted in fresh YPD to an OD600 of 0.1 and grown at 30°C until an OD600 of 1–1.1 was reached. Cultures were split and then potassium sorbate (BBI, USA) was added at a final concentration of 8 mM to one half of the culture. After 20 min, both untreated and treated cultures were harvested by centrifugation at room temperature (2 min, 4000×g), and cells were immediately washed in ice-cold water, reharvested at 4°C, snap-frozen in liquid nitrogen and stored at −80°C. Total RNA was prepared using an SV Total RNA Isolation System (Promega, USA) according to the manufacturer's instructions. Residual DNA was digested with RNase-free DNase (DNase I, TaKaRa, China) at 37°C for 30 min. After heat inactivation for 10 min at 65°C in 2 mM EDTA, 1.5 µl total RNA solution was removed for quantification.
Gene Expression Profiling via Multigene Concatemers (MgC-GEP)
According to the principles of GenomeLab™ GeXP Genetic Analysis System, we designed 20 pairs of multiplex gene-specific primer with a tag for the 20 genes in S. cerevisiae strain W303-1A and 1 pair of universal primer by using Beacon Designer 2.0 software (Bio-Rad, USA) (Table S1). Forward gene-specific primers consisted of 16–20 nucleotides corresponding to the target gene coupled to an 18-nucleotide universal forward tag sequence with a BamHI site. Reverse gene-specific primers consisted of 16–20 nucleotides complementary to the target gene coupled to a 19-nucleotide universal reverse tag sequence with HindIII site. The pair of universal primers is the 18-nucleotide universal forward tag sequence and the 19-nucleotide universal reverse tag sequence. The average Tm of all 20 gene-specific primers is 67.3±1.8°C with difference from each other within 5°C. And PCR products are 85±7 bp in length. The amplification efficiencies of specific primers were examined using real-time RT-PCR (Table S3).
For each sample, reverse transcription (RT) was followed by PCR (Fig. 1). RT reactions mixtures (25 µl) contained 500 ng RNA, 0.16 µM reverse gene-specific primer mix, 200 U reverse transcriptase (Promega, USA), 25 U RNase inhibitor (TaKaRa, China), 5 µl M-MLV 5×reaction Buffer, and 0.5 mM each dNTP. RT reactions were incubated at 70°C for 5 min, 42°C for 60 min. Subsequent PCR was done with each reaction containing 2 µl RT reaction, 0.02 µM forward primer set mix, 2.5 U Taq DNA polymerase (Bioflux, Japan), 0.2 mM each dNTP, and 10×PCR buffer (Bioflux, Japan) containing 5 mM MgCl2, 10 mM Tris-HCl, 50 mM KCl, 1 µM universal forward primer, and 1 µM universal reverse primer. Amplification conditions consisted of initial denaturation at 95°C for 5 min, followed by 28 cycles of 94°C for 30 s and 53°C for 30 s, 72°C for 35 s ending in a single extension cycle of 72°C for 5 min. The PCR product was extracted with PC8 (phenol∶chloroform 1∶1, pH 8.0) and precipitated with isopropanol, and dissolved in Lo TE buffer (3 mM Tris-HCl, pH 7.5; 0.2 mM EDTA, pH 7.5) after washed by 70% ethanol. The purified and concentrated product was digested by HindIII and BamHI for 12 h and treated by chloroform extraction and isopropanol precipitation, and dissolved in Tris-EDTA (TE) buffer. The digested product was separated by 3% agarose gel electrophoresis. The gel containing of digested target bands was cut and centrifuged by spin column. The product was precipitated by ethanol and dissolved in Lo TE buffer. The digested products were ligated to produce concatemers by 5 U T4 DNA ligase (TaKaRa, China) and 15% PEG6000 at 16°C for 4 h. The concatemers were separated by 1.5% agarose gel electrophoresis. The region 500 bp–1200 bp was isolated and cloned in the pUC19T-vector digested by HindIII and BamHI. Finally, the recombinant plasmids were transformed into E. coli competent cells.
We randomly selected 300 clones from each sample and inserts were sequenced using ABI PRISM 3730 automatic DNA sequence systems (SANGON Sequencing Service, Shanghai China). The sequencing primers were M13-47′ (5′-CGCCAGGGTTTTCCCAGTCACGAC-3′) and RV-M (5′-GAGCGGATAACAATTTCACACAGG-3′). The sequence and occurrence of each gene were determined and the Act1 gene encoding Act1p, which was a ubiquitous protein involved in the filament formation, was as the internal control. The sequences were analyzed by using the local BLAST program. All samples were analyzed in triplicate.
Real-time RT-PCR analysis
For real-time RT-PCR, the cDNA was synthesized from 1 µg of DNaseI-treated total RNA with the anchored oligo-dT primer following the manufacturer's protocol (Promega, USA). Twenty pairs of specific primers for the 20 genes in S. cerevisiae strain W303-1A were designed using Beacon Designer 2.0 software (Bio-Rad, USA) (Table S2). Real-time RT-PCR was performed using the SYBR-Green PCR Master Mix kit (Bio-Rad, USA) in the Light Cycler (Bio-Rad, USA). A serial dilution of the cDNA was used as standard curve to optimize the amplification efficiency with each primer pair. Three technical replicates were run for each of the standards. The cycling conditions were 95°C for 15 sec followed by 40 cycles of 95°C for 15 sec, 53.5°C for 15 sec and 72°C for 15 sec. Amplification specificity was confirmed by generating a melting curve of the PCR products. The amplified fragments were also verified by gel electrophoresis. Act1, which encoded Act1p, a ubiquitous protein involved in the filament formation, was amplified as the internal control. Relative target gene expression was determined with the comparative cycle threshold (CT) method. The ΔCT value was calculated by subtracting the target CT for each sample from its Act1 CT value. Every RT-PCR experiment was repeated with three biological samples and each sample was run in triplicate. The relative expression level of mRNA was analyzed between the weak acid-induced samples and non-induced samples with DPS statistical analysis software.
Scheme of MgC-GEP
A schematic diagram of the technique is shown as Fig. 1. First, total RNA extracted from samples was reverse transcribed using 20 reverse primers each containing 19–22 nucleotides complementary to the target gene coupled with a 19-nucleotide universal reverse sequence to obtain the cDNA. The cDNA was PCR-amplified during in the initial 1–3 cycles using the specific primers to synthesize the dozens of gene-amplification products, each of which contained universal tags with specific restriction digestion sites for enzymes a and b at both termini. In this study, the universal tags included the specific restriction digestion sites of BamHI and HindIII. Subsequent PCR amplifications (cycles 4–28) used universal forward and reverse primers to yield amplification products corresponding to each of the 20 specific genes. These two steps effectively condensed mRNA and reduced nonspecific amplifications. The tags corresponding to each of the 20 specific genes were digested with restriction enzyme a and b. The digested products were ligated to produce concatemers with T4 DNA ligase. The concatemers were cloned into the vector digested with restriction enzyme a and b for sequencing.
Validation of MgC-GEP
To identify potentially protective genes induced by sorbate, the whole-genome transcriptional profile has been studied by whole-genome microarrays . According to the results of the study, we chose to characterize the gene transcription of 20 genes including 10 up-regulated genes (YPL122C, YNR030W, YDR343C, YGR088W, YPR149W, YCL040W, YBR054W, YNR001C, YDR533C, YDL222C), 9 down-regulated genes (YML123C, YEL046C, YLR180W, YLR355C, YLR419W, YLR300W, YNL300W, YLR372W, YAL059W) and Act1 as the internal control in S. cerevisiae that were under the weak acid stress to evaluate the reliability of MgC-GEP conveniently. The target genes were amplified via multiplex RT-PCR (lanes 1 in Fig. 2A) and then the RT-PCR products digested by BamHI/HindIII (lanes 2 in Fig. 2A). After that, the digested 60–70-bp tags were ligated to produce concatemers and 500- to 1200-bp concatemers were collected (Fig. 2B) and cloned into the pUC19 T-vector (TaKaRa, China) digested by BamHI/HindIII. Insert size of 300 clones selected randomly was estimated by PCR analysis (Fig. 2C). The size of the inserts varied from 500 to 1200 bp in length, with an average length of 800 bp. This result demonstrated that there were at least 10 tags in one clone. Inserts were sequenced using ABI PRISM 3730 automatic DNA sequence systems (Sangon, China). The sequence and occurrence of each gene were determined manually and gene expression analysis was performed using the BLAST program.
(A) Detection of the PCR products digested. Lane 1 was the PCR products without digestion. Lane 2 was the PCR products digested with BamHI and HindIII. The RT-PCR products digested and undigested were separated by 3% agarose gel electrophoresis and stained with ethidium bromide. Marker (M) was the pUC18 DNA/MspI (TIANGEN). (B) The digested products were ligated to produce concatemers with T4 DNA ligase. The concatemers were analyzed by using 1.5% agarose gel and stained with ethidium bromide. Maker (M) was the MIII (DingGuo). (C) Insert size of 15 clones was estimated by PCR analysis. The PCR products were analyzed by using 1% agarose gel and stained with ethidium bromide. Lanes 1 to 15 were the different clones selected randomly. Marker (M) was the MIII (DingGuo).
Using MgC-GEP, we found that the expression patterns of 16 genes in 20 selected genes were consistent with the results of previously reported microarray analysis . Seven genes (YNR030W, YDR343C, YGR088W, YBR054W, YNR001C, YDR533C, YDL222C) were up-regulated with weak acid induction and nine genes (YML123C, YEL046C, YLR180W, YLR355C, YLR419W, YLR300W, YNL300W, YLR372W, YAL059W) that were repressed (Fig. 3). Notably, three genes (YPL122C, YCL040W and YPR149W) were proven to be up-regulated during weak acid induction using microarray analysis . However, in MgC-GEP analysis, YPL122C and YCL040W were down-regulated and the transcription level of YPR149W did not noticeably change during weak acid induction. To validate MgC-GEP, we also detected the transcription of the 20 selected genes using real time RT-PCR. The results showed that the expression patterns of 20 genes analyzed by real time RT-PCR were consistent with the results of MgC-GEP analysis. These results demonstrated that MgC-GEP can accurately determine the transcript levels of at least 20 genes at once.
Yeast cells of S. cerevisiae W303-1A strain from overnight cultures in YPD were diluted in fresh YPD to an OD600 of 0.1 and grown at 30°C until an OD600 of 1–1.1 was reached. Cultures were split and potassium sorbate (BBI) was added at a final concentration of 8 mM to one half of the culture. After 20 min, both untreated and treated cultures were harvested by centrifugation at room temperature (2 min, 4000×g), and cells were immediately washed in ice-cold water, reharvested at 4°C, snap-frozen in liquid nitrogen and saved at −80°C. *Microarray data was cited from previous research .
In this study, we present a novel method for multigene expression profiling, designated as MgC-GEP. To validate MgC-GEP, we identified the transcript levels of 20 genes stimulated with weak acid in S. cerevisiae using the MgC-GEP and real-time RT-PCR, respectively. Additionally, we have successfully detected the transcription of 8 genes of Toll pathway of Locusta migratoria manilensis in eight different tissues at six time points using MgC-GEP (unpublished data). It demonstrated that MgC-GEP could exactly determine the transcript levels of a moderate number of genes at once effectively.
In MgC-GEP, two initial steps were performed to synthesize cDNA using specific primers containing universal tags with specific digestion sites at both termini. The universal tags, containing the specific digestion sites, will facilitate the formation of concatermers. It offers another advantage, namely, the simplification of the subsequent step by making it possible to analyze the fragments by polyacrylamide gel electrophoresis or agarose gel electrophoresis. Importantly, MgC-GEP is based on sequencing technique and can be freely applied in most laboratories without the acquisition of specific equipments.
Multiple primer design is a very important step in MgC-GEP. First of all, the calculated primer melting temperature (Tm) and the size of PCR products should be similar so that all primers anneal at same temperatures during the PCR temperature cycling for better balance between PCR products in a multiplex reaction . Moreover, high quality primers are essential for successful multiplex amplification reactions. During 3–28 cycles in the amplification, the universal primers are more competitive than the chimeric primers due to their higher abundance. At this time, different genes are amplified with the same universal primer pairs. Therefore, the chimeric primers and universal primers could lead to the same efficiency for each amplicon.
When choosing a method for quantifying gene expression, there are several basic considerations, including specificity, throughput, sensitivity, cost and data analysis. Like SAGE and SuperSAGE, MgC-GEP is based on the sequential analysis of short cDNA sequence tags , . Each tag is derived from a defined position within a transcript. For specificity, the size (>60 bp) of the tags is long enough to be identified as the corresponding gene and the frequency of each tag numbered provides an accurate measurement of its expression level. Closely related gene sequences could be discriminated through precise primer designing and sequencing in MgC-GEP. Specifically, this method could allow the accurate determination of the expression levels of multiple transcripts that could lead to the false positive of an expression differential by hybridization due to the high identity at nucleotide level. For sensitivity, it is possible to detect poorly expressed genes with high sensitivity using MgC-GEP since it is a PCR-based technique. For throughput, the transcript levels of at least 20 genes could be monitored at once using MgC-GEP. It was more practical to monitor the transcript levels of 10–50 genes under the various conditions or at different times than the real-time RT-PCR, DNA microarray and SAGE. For real-time multiplex RT-PCR, if one template is much more abundant than the other templates in the same reaction tube, the reaction components will be depleted before the lower-abundance targets have amplified sufficiently to be detected . For DNA microarray and SAGE, though thousands of genes could be determined, the large cost of multiple samples may be the main constraint . For example, detecting the expression profiling of 10–50 genes in kinetics studies or comparing the effects of a large number of drugs is costly. The GeXP method is a good choice for multigene expression profiling of multiple samples, but the necessary equipments such as a fluorescence scanner (detector) and a capillary electrophoresis machine are required to generate the data , . This is inconvenient for laboratories that do not possess this equipment. MgC-GEP can be applied in most laboratories and done readily without additional cost and equipment. Another advantage is in data analysis, MgC-GEP can use Blast analysis to detect and count tags from sequence files, thus can analyze the experimental data without the necessity of professional software. Although there are distinct advantages to MgC-GEP, there are also two limitations: MgC-GEP does not determine the absolute expression level of transcripts but the relative abundance of selected transcripts, and the sensitivity of MgC-GEP depends on number of clones sequenced, thus detection of rare transcripts would sequence more clones which will increase the cost.
In conclusion, MgC-GEP adopts covnentional RT-PCR, restriction enzyme digestion, concatemer ligation, bacterial transformation and DNA sequencing to assess mRNA profiles without using high-throughput expensive methodologies such as microarray hybridization or deep sequencing. No specific equipment is required in this method. This strategy could be of great benefit for labs with limited funds or limited access to microarray or next-generation sequencing facilities. Thus, MgC-GEP should be a potentially powerful tool for multigene expression profiling in different tissues, during development, or during specific pathologies in both basic and pharmaceutical research.
Multiplex gene-specific primers and universal primers. *Underlined sequences are restriction sites. Italic letters are the universal sequences.
Primer sequence for real-time RT-PCR.
Conceived and designed the experiments: YX. Performed the experiments: KJ XZ. Analyzed the data: KJ XZ. Contributed reagents/materials/analysis tools: YX. Wrote the paper: KJ XZ YX.
- 1. Gregory SG, Sekhon M, Schein J, Zhao S, Osoegawa K, et al. (2002) A physical map of the mouse genome. Nature 418: 743–750.SG GregoryM. SekhonJ. ScheinS. ZhaoK. Osoegawa2002A physical map of the mouse genome.Nature418743750
- 2. Li R, Fan W, Tian G, Zhu H, He L, et al. (2009) The sequence and de novo assembly of the giant panda genome. Nature 463: 311–317.R. LiW. FanG. TianH. ZhuL. He2009The sequence and de novo assembly of the giant panda genome.Nature463311317
- 3. Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, et al. (2001) Initial sequencing and analysis of the human genome. Nature 409: 860–921.ES LanderLM LintonB. BirrenC. NusbaumMC Zody2001Initial sequencing and analysis of the human genome.Nature409860921
- 4. Dean RA, Talbot NJ, Ebbole DJ, Farman ML, Mitchell TK, et al. (2005) The genome sequence of the rice blast fungus Magnaporthe grisea. Nature 434: 980–986.RA DeanNJ TalbotDJ EbboleML FarmanTK Mitchell2005The genome sequence of the rice blast fungus Magnaporthe grisea.Nature434980986
- 5. Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, et al. (2000) The genome sequence of Drosophila melanogaster. Science 287: 2185–2195.MD AdamsSE CelnikerRA HoltCA EvansJD Gocayne2000The genome sequence of Drosophila melanogaster.Science28721852195
- 6. Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, et al. (2002) The genome sequence of the malaria mosquito Anopheles gambiae. Science 298: 129–149.RA HoltGM SubramanianA. HalpernGG SuttonR. Charlab2002The genome sequence of the malaria mosquito Anopheles gambiae.Science298129149
- 7. Sasaki T, Matsumoto T, Yamamoto K, Sakata K, Baba T, et al. (2002) The genome sequence and structure of rice chromosome 1. Nature 420: 312–316.T. SasakiT. MatsumotoK. YamamotoK. SakataT. Baba2002The genome sequence and structure of rice chromosome 1.Nature420312316
- 8. Nene V, Wortman JR, Lawson D, Haas B, Kodira C, et al. (2007) Genome sequence of Aedes aegypti, a major arbovirus vector. Science 316: 1718–1723.V. NeneJR WortmanD. LawsonB. HaasC. Kodira2007Genome sequence of Aedes aegypti, a major arbovirus vector.Science31617181723
- 9. Hughes TR, Marton MJ, Jones AR, Roberts CJ, Stoughton R, et al. (2000) Functional discovery via a compendium of expression profiles. Cell 102: 109–126.TR HughesMJ MartonAR JonesCJ RobertsR. Stoughton2000Functional discovery via a compendium of expression profiles.Cell102109126
- 10. Cebeci O, Budak H (2009) Global expression patterns of three Festuca species exposed to different doses of glyphosate using the affymetrix geneChip wheat genome array. Comp Funct Genomics 2009: 505701.O. CebeciH. Budak2009Global expression patterns of three Festuca species exposed to different doses of glyphosate using the affymetrix geneChip wheat genome array.Comp Funct Genomics2009505701
- 11. Lomenick B, Hao R, Jonai N, Chin RM, Aghajan M, et al. (2009) Target identification using drug affinity responsive target stability (DARTS). Proc Natl Acad Sci USA 106: 21984–21989.B. LomenickR. HaoN. JonaiRM ChinM. Aghajan2009Target identification using drug affinity responsive target stability (DARTS).Proc Natl Acad Sci USA1062198421989
- 12. Ji RR, de Silva H, Jin Y, Bruccoleri RE, Cao J, et al. (2009) Transcriptional profiling of the dose response: a more powerful approach for characterizing drug activities. PLoS Comput Biol 5: e1000512.RR JiH. de SilvaY. JinRE BruccoleriJ. Cao2009Transcriptional profiling of the dose response: a more powerful approach for characterizing drug activities.PLoS Comput Biol5e1000512
- 13. Hu G, Agarwal P (2009) Human disease-drug network based on genomic expression profiles. PLoS One 4: e6536.G. HuP. Agarwal2009Human disease-drug network based on genomic expression profiles.PLoS One4e6536
- 14. Golub TR, Slonim DK, Tamayo P, Huard C, Gaasenbeek M, et al. (1999) Molecular classification of cancer: class discovery and class prediction by gene expression monitoring. Science 286: 531–537.TR GolubDK SlonimP. TamayoC. HuardM. Gaasenbeek1999Molecular classification of cancer: class discovery and class prediction by gene expression monitoring.Science286531537
- 15. Alizadeh AA, Eisen MB, Davis RE, Ma C, Lossos IS, et al. (2000) Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature 403: 503–511.AA AlizadehMB EisenRE DavisC. MaIS Lossos2000Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling.Nature403503511
- 16. Edelman L, Toia G, Geman D, Zhang W, Price N (2009) Two-transcript gene expression classifiers in the diagnosis and prognosis of human diseases. BMC Genomics 10: 583.L. EdelmanG. ToiaD. GemanW. ZhangN. Price2009Two-transcript gene expression classifiers in the diagnosis and prognosis of human diseases.BMC Genomics10583
- 17. Perou CM, Jeffrey SS, van de Rijn M, Rees CA, Eisen MB, et al. (1999) Distinctive gene expression patterns in human mammary epithelial cells and breast cancers. Proc Natl Acad Sci USA 96: 9212–9217.CM PerouSS JeffreyM. van de RijnCA ReesMB Eisen1999Distinctive gene expression patterns in human mammary epithelial cells and breast cancers.Proc Natl Acad Sci USA9692129217
- 18. Schena M, Shalon D, Davis RW, Brown PO (1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270: 467–470.M. SchenaD. ShalonRW DavisPO Brown1995Quantitative monitoring of gene expression patterns with a complementary DNA microarray.Science270467470
- 19. Brenner S, Johnson M, Bridgham J, Golda G, Lloyd DH, et al. (2000) Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays. Nat Biotechnol 18: 630–634.S. BrennerM. JohnsonJ. BridghamG. GoldaDH Lloyd2000Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays.Nat Biotechnol18630634
- 20. Velculescu VE, Zhang L, Vogelstein B, Kinzler KW (1995) Serial analysis of gene expression. Science 270: 484–487.VE VelculescuL. ZhangB. VogelsteinKW Kinzler1995Serial analysis of gene expression.Science270484487
- 21. Matsumura H, Bin Nasir KH, Yoshida K, Ito A, Kahl G, et al. (2006) SuperSAGE array: the direct use of 26-base-pair transcript tags in oligonucleotide arrays. Nat Methods 3: 469–474.H. MatsumuraKH Bin NasirK. YoshidaA. ItoG. Kahl2006SuperSAGE array: the direct use of 26-base-pair transcript tags in oligonucleotide arrays.Nat Methods3469474
- 22. Wang Z, Gerstein M, Snyder M (2009) RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet 10: 57–63.Z. WangM. GersteinM. Snyder2009RNA-Seq: a revolutionary tool for transcriptomics.Nat Rev Genet105763
- 23. Wittwer CT, Herrmann MG, Gundry CN, Elenitoba-Johnson KS (2001) Real-time multiplex PCR assays. Methods 25: 430–442.CT WittwerMG HerrmannCN GundryKS Elenitoba-Johnson2001Real-time multiplex PCR assays.Methods25430442
- 24. Erdner D, Anderson D (2006) Global transcriptional profiling of the toxic dinoflagellate Alexandrium fundyense using Massively Parallel Signature Sequencing. BMC Genomics 7: 88.D. ErdnerD. Anderson2006Global transcriptional profiling of the toxic dinoflagellate Alexandrium fundyense using Massively Parallel Signature Sequencing.BMC Genomics788
- 25. Daxboeck F, Krause R, Wenisch C (2003) Laboratory diagnosis of Mycoplasma pneumoniae infection. Clin Microbiol Infect 9: 263–273.F. DaxboeckR. KrauseC. Wenisch2003Laboratory diagnosis of Mycoplasma pneumoniae infection.Clin Microbiol Infect9263273
- 26. Zarlenga DS, Higgins J (2001) PCR as a diagnostic and quantitative technique in veterinary parasitology. Vet Parasitol 101: 215–230.DS ZarlengaJ. Higgins2001PCR as a diagnostic and quantitative technique in veterinary parasitology.Vet Parasitol101215230
- 27. Kuo WP, Whipple ME, Sonis ST, Ohno-Machado L, Jenssen TK (2002) Gene expression profiling by DNA microarrays and its application to dental research. Oral Oncol 38: 650–656.WP KuoME WhippleST SonisL. Ohno-MachadoTK Jenssen2002Gene expression profiling by DNA microarrays and its application to dental research.Oral Oncol38650656
- 28. Yamamoto M, Wakatsuki T, Hada A, Ryo A (2001) Use of serial analysis of gene expression (SAGE) technology. J Immunol Methods 250: 45–66.M. YamamotoT. WakatsukiA. HadaA. Ryo2001Use of serial analysis of gene expression (SAGE) technology.J Immunol Methods2504566
- 29. Reinartz J, Bruyns E, Lin JZ, Burcham T, Brenner S, et al. (2002) Massively parallel signature sequencing (MPSS) as a tool for in-depth quantitative gene expression profiling in all organisms. Brief Funct Genomic Proteomic 1: 95–104.J. ReinartzE. BruynsJZ LinT. BurchamS. Brenner2002Massively parallel signature sequencing (MPSS) as a tool for in-depth quantitative gene expression profiling in all organisms.Brief Funct Genomic Proteomic195104
- 30. Fan X, Shi L, Fang H, Cheng Y, Perkins R, et al. DNA microarrays are predictive of cancer prognosis: a re-evaluation. Clin Cancer Res 16: 629–636.X. FanL. ShiH. FangY. ChengR. PerkinsDNA microarrays are predictive of cancer prognosis: a re-evaluation.Clin Cancer Res16629636
- 31. Zembutsu H, Ohnishi Y, Tsunoda T, Furukawa Y, Katagiri T, et al. (2002) Genome-wide cDNA microarray screening to correlate gene expression profiles with sensitivity of 85 human cancer xenografts to anticancer drugs. Cancer Res 62: 518–527.H. ZembutsuY. OhnishiT. TsunodaY. FurukawaT. Katagiri2002Genome-wide cDNA microarray screening to correlate gene expression profiles with sensitivity of 85 human cancer xenografts to anticancer drugs.Cancer Res62518527
- 32. van't Veer LJ, Dai H, van de Vijver MJ, He YD, Hart AA, et al. (2002) Gene expression profiling predicts clinical outcome of breast cancer. Nature 415: 484–485.LJ van't VeerH. DaiMJ van de VijverYD HeAA Hart2002Gene expression profiling predicts clinical outcome of breast cancer.Nature415484485
- 33. Smith GW, Rosa GJ (2007) Interpretation of microarray data: trudging out of the abyss towards elucidation of biological significance. J Anim Sci 85: E20–23.GW SmithGJ Rosa2007Interpretation of microarray data: trudging out of the abyss towards elucidation of biological significance.J Anim Sci85E2023
- 34. Liang P (2007) MAQC papers over the cracks. Nat Biotechnol 25: 27–28.P. Liang2007MAQC papers over the cracks.Nat Biotechnol252728
- 35. Wang SM (2006) Applying the SAGE technique to study the effects of electromagnetic field on biological systems. Proteomics 6: 4765–4768.SM Wang2006Applying the SAGE technique to study the effects of electromagnetic field on biological systems.Proteomics647654768
- 36. Gunson R, Gillespie G, W FC (2003) Optimisation of PCR reactions using primer chessboarding. J Clin Virol 26: 369–373.R. GunsonG. GillespieFC W2003Optimisation of PCR reactions using primer chessboarding.J Clin Virol26369373
- 37. Henegariu O, Heerema NA, Dlouhy SR, Vance GH, Vogt PH (1997) Multiplex PCR: critical parameters and step-by-step protocol. Biotechniques 23: 504–511.O. HenegariuNA HeeremaSR DlouhyGH VancePH Vogt1997Multiplex PCR: critical parameters and step-by-step protocol.Biotechniques23504511
- 38. Nagel MA, Gilden D, Shade T, Gao B, Cohrs RJ (2009) Rapid and sensitive detection of 68 unique varicella zoster virus gene transcripts in five multiplex reverse transcription-polymerase chain reactions. J Virol Methods 157: 62–68.MA NagelD. GildenT. ShadeB. GaoRJ Cohrs2009Rapid and sensitive detection of 68 unique varicella zoster virus gene transcripts in five multiplex reverse transcription-polymerase chain reactions.J Virol Methods1576268
- 39. Rai AJ, Kamath RM, Gerald W, Fleisher M (2009) Analytical validation of the GeXP analyzer and design of a workflow for cancer-biomarker discovery using multiplexed gene-expression profiling. Anal Bioanal Chem 393: 1505–1511.AJ RaiRM KamathW. GeraldM. Fleisher2009Analytical validation of the GeXP analyzer and design of a workflow for cancer-biomarker discovery using multiplexed gene-expression profiling.Anal Bioanal Chem39315051511
- 40. Schuller C, Mamnun YM, Mollapour M, Krapf G, Schuster M, et al. (2004) Global phenotypic analysis and transcriptional profiling defines the weak acid stress response regulon in Saccharomyces cerevisiae. Mol Biol Cell 15: 706–720.C. SchullerYM MamnunM. MollapourG. KrapfM. Schuster2004Global phenotypic analysis and transcriptional profiling defines the weak acid stress response regulon in Saccharomyces cerevisiae.Mol Biol Cell15706720
- 41. Schoske R, Vallone PM, Ruitberg CM, Butler JM (2003) Multiplex PCR design strategy used for the simultaneous amplification of 10 Y chromosome short tandem repeat (STR) loci. Anal Bioanal Chem 375: 333–343.R. SchoskePM ValloneCM RuitbergJM Butler2003Multiplex PCR design strategy used for the simultaneous amplification of 10 Y chromosome short tandem repeat (STR) loci.Anal Bioanal Chem375333343