Differentiating cerebral malaria (CM) from other causes of serious illness in African children is problematic, owing to the non-specific nature of the clinical presentation and the high prevalence of incidental parasitaemia. CM is associated with endothelial activation. In this study we tested the hypothesis that endothelium-derived biomarkers are associated with the pathophysiology of severe malaria and may help identify children with CM.
Methods and Findings
Plasma samples were tested from children recruited with uncomplicated malaria (UM; n = 32), cerebral malaria with retinopathy (CM-R; n = 38), clinically defined CM without retinopathy (CM-N; n = 29), or non-malaria febrile illness with decreased consciousness (CNS; n = 24). Admission levels of angiopoietin-2 (Ang-2), Ang-1, soluble Tie-2 (sTie-2), von Willebrand factor (VWF), its propeptide (VWFpp), vascular endothelial growth factor (VEGF), soluble ICAM-1 (sICAM-1) and interferon-inducible protein 10 (IP-10) were measured by ELISA. Children with CM-R had significantly higher median levels of Ang-2, Ang-2:Ang-1, sTie-2, VWFpp and sICAM-1 compared to children with CM-N. Children with CM-R had significantly lower median levels of Ang-1 and higher median concentrations of Ang-2:Ang-1, sTie-2, VWF, VWFpp, VEGF and sICAM-1 compared to UM, and significantly lower median levels of Ang-1 and higher median levels of Ang-2, Ang-2:Ang-1, VWF and VWFpp compared to children with fever and altered consciousness due to other causes. Ang-1 was the best discriminator between UM and CM-R and between CNS and CM-R (areas under the ROC curve of 0.96 and 0.93, respectively). A comparison of biomarker levels in CM-R between admission and recovery showed uniform increases in Ang-1 levels, suggesting this biomarker may have utility in monitoring clinical response.
These results suggest that endothelial proteins are informative biomarkers of malarial disease severity. These results require validation in prospective studies to confirm that this group of biomarkers improves the diagnostic accuracy of CM from similar conditions causing fever and altered consciousness.
Citation: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, et al. (2010) Endothelium-Based Biomarkers Are Associated with Cerebral Malaria in Malawian Children: A Retrospective Case-Control Study. PLoS ONE 5(12): e15291. doi:10.1371/journal.pone.0015291
Editor: Steffen Borrmann, KEMRI/Wellcome Trust Research Programme, Kenya
Received: August 5, 2010; Accepted: November 10, 2010; Published: December 29, 2010
Copyright: © 2010 Conroy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was funded in part by a Canadian Institutes of Health Research (CIHR) Team Grant in Malaria (KCK), CIHR MOP-13721 (KCK), Genome Canada through the Ontario Genomics Institute (KCK), CIHR Canada Research Chair (KCK), CIHR studentship (ALC), a Defense Advanced Research Projects Agency grant (KCK) and a kind donation from Mr. D. Kirkwood. The funding agencies had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Enrollment, clinical characterization and sampling of patients were funded by grants from The Wellcome Trust, United Kingdom, and the National Institutes of Health, United States of America (5R01AI034969).
Competing interests: KCK and AC are listed as inventors on a patent owned by the University Health Network related to host biomarkers of malaria (date of filing: August 8, 2010; Provisional Patent Serial Number: 61/371,401). This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.
Differentiating cerebral malaria (CM) from other conditions causing fever and altered consciousness is a clinical challenge, owing to the non-specific clinical presentations of CM (fever, coma, convulsions) and the high prevalence of incidental parasitaemia in malaria-endemic areas , , , . In a study of African children diagnosed with CM, approximately one quarter were shown to have alternative causes for their neurological syndrome at post-mortem examination . These findings indicate that CM is over-diagnosed, a situation that is likely to have serious consequences for children in whom other treatable or life-threatening conditions are not identified , . There is a clear need for a diagnostic test that could distinguish CM from other conditions causing encephalopathy in malaria-endemic areas. In comatose African children, a distinctive retinopathy consisting of haemorrhages, patchy retinal whitening and vessel changes is strongly associated with malaria being the only identifiable cause of death .
Features of severe P. falciparum malaria include the adhesion of mature parasitized erythrocytes to the microvasculature of vital organs and acute endothelial activation (reviewed in ; ). Exocytosis of Weibel-Palade bodies (WPBs) occurs in association with endothelial activation and the products of WPBs have been identified as biomarkers of malarial disease severity , , . WPBs release bioactive products, including von Willebrand factor (VWF), its propeptide (VWFpp), and angiopoietin-2 (Ang-2) into the systemic circulation. Together with vascular endothelial growth factor (VEGF), the angiogenic factors angiopoietin-1 (Ang-1) and Ang-2, are major regulators of the vascular inflammatory response, endothelial activation and endothelial integrity , . Ang-1 is constitutively released from perivascular cells including pericytes and smooth muscle cells and signals through the Tie-2 receptor to maintain vascular quiescence and stability. Ang-2 antagonizes Ang-1 function resulting in endothelial activation and increased vascular permeability. Ang-2 sensitizes the endothelium to sub-threshold levels of tumour necrosis factor, resulting in increased expression of adhesion molecules such as ICAM-1 to which parasitized erythrocytes bind . VEGF induces WPB exocytosis, mediates Tie-2 shedding, and regulates Ang-1 and Ang-2 function , . Tie-2 is the cognate receptor for Ang-1 and Ang-2 and the soluble form of the receptor, sTie-2, can bind the angiopoietins and regulate their function. WPBs are also an important source of VWF, particularly ultralarge multimers (ULVWF) that are considered biologically hyperactive with respect to their enhanced binding avidity for collagen and platelets . Severe malaria has been associated with increased levels of VWF and ULVWF multimers and decreased levels of the regulatory VWF-specific cleaving protease ADAMTS13 (A disintegrin and metalloprotease with thrombospondin type-1 repeats) . ICAM-1 is a receptor for the cytoadherence of mature parasitized erythrocytes in the cerebral microvasculature and its soluble form (sICAM-1) has been used as a marker of endothelial activation and severe malaria , , . In addition to the molecular markers and regulators of endothelial quiescence and activation, IP-10, an interferon-gamma inducible chemokine involved in recruitment of activated Th1 cells, has been reported as a biomarker of fatal CM in studies from India and Ghana , .
Reliable diagnostic and prognostic biomarkers for CM and other forms of severe malaria may improve clinical management, resource allocation and outcome of serious childhood illness. The aim of this study was to evaluate the ability of endothelial biomarkers to discriminate between different clinical disease states in malaria and between cerebral malaria and other conditions associated with fever and altered consciousness in Malawian children. We show that endothelium-based proteins are informative biomarkers of disease severity and clinical response and that a panel of biomarkers can discriminate between retinopathy positive CM and uncomplicated malaria or other CNS infections with a high degree of accuracy. Further, we demonstrate that a distinctive set of endothelium-based proteins is associated with retinopathy in a group of children with coma and parasitaemia.
Ethical approval for this study was granted from The College of Medicine Research Committee in Blantyre, Malawi (COMREC) and all parents or guardians gave written informed consent for children to enter the study.
This study was nested within prospective studies examining the pathogenesis and management of CM and central nervous system infections , , . Convenience samples were selected for children between 1 month and 14 years of age presenting with fever to the Queen Elizabeth Central Hospital (QECH) in Blantyre, Malawi between 1997 and 2009, who met the eligibility criteria for the clinical syndromes and had sufficient plasma samples available. Admission lithium heparin plasma samples were obtained from children after their parents or guardians had given their informed consent. Clinical and demographic data were collected from cases and controls at the time of blood collection, and all subsequent analyses were carried out blind to these details. Efforts were made to minimize bias by selecting subjects from a single population (children presenting to QECH), and by using two types of controls, uncomplicated malaria illness (outpatient) and non-malarial febrile illness with decreased consciousness (inpatient). All participants received standard treatment, including antimalarial and/or antibacterial therapy as indicated, according to Malawian National guidelines.
After admission of a child with altered consciousness, the patient's pupils were dilated by application of drops (tropicamide and phenylephrine) and the fundi were examined by direct and indirect ophthalmoscopy. The findings of an ophthalmologist or experienced clinician were recorded on standardized forms. Retinopathy was defined by the presence of any one or more of the following retinal findings: white-centered haemorrhages, retinal whitening, or vessel changes, with or without papilloedema, as previously described , . Papilloedema alone did not constitute retinopathy.
Definitions of clinical syndromes
Cerebral Malaria (CM).
Children meeting the case definition for CM ,  including P. falciparum asexual parasitaemia, a Blantyre coma score ≤2 with no improvement following correction of hypoglycemia, and no evidence of an alternative cause for coma including meningitis on examination of cerebrospinal fluid, were eligible for enrolment. Children's fundi were examined and they were classified as retinopathy positive (CM-R) or retinopathy negative (CM-N). CM-R children were considered to be confirmed CM and were used for all analyses comparing clinical groups. Paired admission and 28 day convalescence plasma samples (representing clinical recovery) were collected for each surviving child available for follow-up.
Uncomplicated Malaria (UM).
Children were included in the UM group if they presented to the outpatient clinic at Queen Elizabeth Central Hospital, Blantyre, Malawi with febrile illness and a blood film positive for asexual P. falciparum without another explanation for fever, and with no malarial complications.
Children with non-malarial fever and altered consciousness were included in the study as a further comparator group. Samples were taken from a study looking at suspected central nervous system (CNS) infections (unpublished data). Children with fever or history of fever, a negative malarial smear and at least one of the following: reduced level of consciousness, Blantyre coma score (BCS) ≤4, neck stiffness, photophobia, Kernig's sign, tense fontanelle, focal neurological signs, convulsions, or unexplained irritability in infants, were eligible for enrolment.
Quantification of Biomarkers
Plasma concentrations of biomarkers Ang-1, Ang-2, sTie-2, VEGF-A, IP-10 and sICAM-1 (DuoSets, R&D Systems, Minneapolis, MN), von Willebrand factor (VWF [capture REF P0226, detection REF A0082]: DAKO, Denmark A/S) and von Willebrand factor propeptide (VWFpp [capture CLB-Pro35, detection CLB-Pro 14.3 HRP conjugated]: Sanquin, Netherlands) were measured by ELISA as follows. According to the manufacturer's instructions, capture antibodies were diluted in PBS (Gibco) overnight at 4°C and were washed with PBS 0.05% Tween 20 (Sigma) five times and blocked for a minimum of 2 hours in PBS 1% BSA (reagent diluent). The samples were then diluted in reagent diluent and standard curves were generated using recombinant proteins (R&D Systems). Normal plasma from a pool of 40 healthy adult Caucasian donors served as a standard for VWFpp and VWF. The plasma pool contained 5.5 nM of VWFpp and 49 nM of VWF. Samples were plated in duplicate and incubated overnight at 4°C, washed five times and detection antibodies were added according to manufacturer recommended dilutions for 2 hours at room temperature (RT). For Ang-1 and Ang-2, the detection antibodies were resuspended one hour prior to use with 2% heat inactivated goat or mouse serum respectively. Following wash steps (7×), VWF and VWFpp were developed using TMB (eBioscience) and the reaction was stopped using 2N H2SO4. The plate was read at 450 nM (Dynex Technologies Opsys MR plate reader) and concentrations were extrapolated from the standard curve (4-PL) using revelation Quicklink software (v4.04). The ELISA assays from R&D systems were washed (7×) and Extravidin-Alkaline phosphatase (AP) (Sigma) was added 1∶1000 to each well for 1 hour at RT. The plates were then washed a final time (7× in PBS 0.05% Tween 20 and 2× in deionized water) before adding the substrate p-nitrophenyl phosphate (pNPP) (Sigma). The plates were read at 405 nM and concentrations were extrapolated as above.
Data were analyzed in GraphPad Prism v5.0 and SPSS v16.0. All analyses were non-parametric with Spearman's correlation for two-way correlations between biomarkers, Mann-Whitney U tests to compare biomarkers between groups with Holms correction for multiple testing, and receiver operating characteristic (ROC) curves to assess the diagnostic accuracy of the tests. Optimal test thresholds were derived mathematically from the ROC curves using the point on the ROC curve with the lowest value for the formula: (1- sensitivity)2 + (1-specificity)2. Wilcoxon matched pairs test was used to compare biomarker levels measured at admission and convalescence.
A total of 123 febrile children with either UM (n = 32), suspected CNS infections (n = 24), or CM (n = 67) were included in the study. Of the children that met study criteria for CM, 38 were retinopathy positive and were classified as retinopathy-validated CM (CM-R); whereas the other 29 had normal ocular fundi (CM-N) . Children with CM-R were significantly younger than children with clinically defined CM without retinopathy. Demographic and clinical data for these children are shown in Table 1.
Endothelial biomarkers differentiate retinopathy positive CM cases from those without retinopathy
Since retinopathy has been established as a discriminant tool in the diagnosis of CM, we examined biomarker levels in children with CM and malaria retinopathy (CM-R) and compared them to children with clinical CM without retinopathy (CM-N). Individually, Ang-2, Ang-2:Ang-1, sTie-2, VWFpp and sICAM-1 were significantly associated with retinopathy (Figure 1, Table 2). For each analyte tested, a receiver operating characteristic (ROC) curve was generated to assess the diagnostic accuracy of the biomarker to discriminate between CM-N and CM-R. The area under the ROC (AUROC) curve was computed and the sensitivity, specificity and positive and negative likelihood ratios were calculated at the optimal biomarker cut-off. sTie-2 and Ang-2 were the best individual predictors of retinopathy with AUROCs of 0.83 (95% CI: 0.73–0.93) and 0.77 (95% CI: 0.65–0.89) respectively.
Graphs showing the median and scatter of plasma biomarkers (A) Ang-2 (ng/mL), (B) Ang-1(ng/mL), (C) Ang-2:Ang-1, (D) VWF propeptide (VWFpp, mmol/L), (E) VWF (mmol/L), (F) sTie-2 (ng/mL), (G) sICAM-1 (ng/mL), (H) VEGF (ng/mL) and (I) IP-10 (ng/mL) levels in retinopathy negative cerebral malaria (CM-N) or retinopathy positive cerebral malaria (CM-R) as measured by ELISA (Mann-Whitney). ns (not significant, p>0.05), *p<0.05 after Holms correction for multiple comparisons (9 pair-wise comparisons). The limit of detection is represented by a dotted line (VEGF).
Endothelial biomarkers differ between CM and UM
We compared admission levels of plasma biomarkers in children with CM-R (n = 38) to children with UM (n = 32). The median concentration of Ang-1 was significantly lower, and median levels of Ang-2:Ang-1, sTie-2, VWFpp, VWF, sICAM, VEGF were significantly higher in patients with CM-R than in patients with UM (Figure 2, Table 3).
Graphs showing the median and scatter of plasma biomarkers (A) Ang-2 (ng/mL), (B) Ang-1(ng/mL), (C) Ang-2:Ang-1, (D) VWF propeptide (VWFpp, mmol/L), (E) VWF (mmol/L), (F) sTie-2 (ng/mL), (G) sICAM-1 (ng/mL), (H) VEGF (ng/mL) and (I) IP-10 (ng/mL) levels in UM, non-malarial febrile illness with decreased consciousness (CNS) or retinopathy validated cerebral malaria (CM-R) as measured by ELISA (Mann-Whitney). ns (not significant, p>0.05), *p<0.05 after Holms correction for multiple comparisons (9 pair-wise comparisons). The limit of detection is represented by a dotted line (VEGF).
ROC curves were generated to assess the diagnostic accuracy of the biomarkers to discriminate between UM and CM-R. The AUROC curve was computed and the sensitivity, specificity and positive and negative likelihood ratios were calculated at the optimal biomarker cut-off (Table 4). Ang-1, sTie-2, VWFpp, VWF, ICAM, VEGF were each able to differentiate between UM and CM-R whereas IP-10 was not. Although Ang-2 on its own was no longer significant after correcting for multiple comparisons, the Ang-2:Ang-1 ratio had an AUROC as good as Ang-1 and resulted in an improved positive likelihood ratio (LR(+)) versus Ang-1 alone [Ang-1 LR(+) = 7.1 compared to Ang-2:Ang-1 LR(+) = 18].
Distinct biomarker profiles in CM differ between retinopathy validated CM and other causes of fever and altered mental status
Distinguishing CM-R from other causes of fever and altered level of consciousness is clinically challenging yet critical for instituting timely, specific, and potentially life-saving treatment. We hypothesized that CM-R is associated with a characteristic pattern of endothelial biomarker abnormalities, which may be clinically informative in distinguishing CM-R from other causes of fever and altered consciousness. Comparing children with CM-R (n = 38) to a control group of children admitted with non-malarial febrile illness and altered level of consciousness (CNS) (n = 24), median Ang-1 levels were lower, and median Ang-2, Ang-2:Ang-1, VWFpp and VWF higher in children with CM-R compared to CNS controls (Figure 2, Table 3).
Using ROC curve analysis, three biomarkers, Ang-1, VWFpp, and VWF, discriminated children with CM-R from children with other suspected CNS infections (Table 5). Median Ang-2 was elevated in CM-R compared to the CNS controls, and the Ang-2:Ang-1 ratio had an AUROC equal to that of Ang-1 alone but with a two-fold increase in the positive likelihood ratio (Table 5). sICAM-1, sTie-2, and VEGF, while useful biomarkers for UM vs. CM-R, did not discriminate between CM-R vs. CNS (Table 5).
Correlation between endothelial biomarkers
Complex interactions have been reported between molecular regulators of endothelial function . Therefore we postulated that significant correlations would exist between endothelial biomarkers. After applying two-way rank correlations we found significant correlations between Ang-2 and sTie-2, VWFpp, VWF, sICAM-1, and IP-10 and inverse correlations between Ang-1, Ang-2, VWF, VWFpp, sICAM-1 and IP-10 (Table 6). Most of the endothelial biomarkers were significantly associated with each other, except for VEGF, which was only associated with IP-10.
Endothelial biomarker levels are associated with clinical recovery
We hypothesized that sequential measurements of endothelial biomarker levels could provide objective and quantitative evidence of clinical recovery and disease resolution. To test this hypothesis, we obtained paired measurements of endothelial biomarkers at admission and at day 28, following treatment and recovery, from 38 survivors of CM-R. Levels of Ang-1 displayed a uniform and consistent increase in all participants, whereas levels of Ang-2, Tie-2, VWFpp, VWF, sICAM-1, and IP-10 decreased with convalescence (Figure 3, Table S1). Overall, VEGF showed a significant increase in levels at convalescence (Table S1). Notably, the Ang-2:Ang-1 ratio showed the most dynamic range between levels at admission and follow-up and there was a universal and consistent decrease in Ang-2:Ang-1 levels associated with clinical recovery.
Plasma levels of biomarkers were measured at admission and 28 days post-treatment in a cohort of retinopathy positive children with cerebral malaria. Wilcoxon signed rank test with Holms correction (9 pair-wise comparisons) was used to compare levels of (A) Ang-2 (ng/mL); sum of signed ranks (W), (W, p-value: 746, p<0.0009); (B) Ang-1 (ng/mL), (W, p-value: −741, p<0.0009); (C) Ang-2:Ang-1, (W, p-value: 741, p<0.0009); (D) VWF propeptide (nM), (W, p-value: 768, p<0.0009); (E) VWF (nM), (W, p-value: 740, p<0.0009); (F) sTie-2 (ng/mL) (W, p-value: 754, p<0.0009); (G) sICAM-1 (ng/mL), (W, p-value: 732, p<0.0009); (H) VEGF (ng/mL), (W, p-value: −388, p = 0.001); and (I) IP-10 (ng/mL), (W, p-value: 607, p<0.0009).
The diagnosis of cerebral malaria in children is clinically challenging since the syndrome may be confused with other causes of fever and altered consciousness. Diagnostic tools that could accurately identify children with “true” CM would enable improved triage and management of these life-threatening infections. Currently retinopathy is the best tool to predict which febrile, comatose children have true CM , –, but it has operational constraints. Alternative methods of discriminating between these groups have not been described. Identifying retinopathy in a comatose child greatly increases the confidence with which the clinical syndrome can be attributed to malaria. Direct ophthalmoscopy through dilated pupils can be used to observe retinal changes, but a more accurate picture is provided by indirect ophthalmoscopy, a procedure that is usually not available where resources are limited. In this study, we tested the hypothesis that plasma biomarkers may function as surrogate markers for malarial retinopathy. We show that several biomarkers (Ang-2, sTie-2, VWFpp and sICAM-1) were significantly associated with retinopathy in children with clinical defined CM (Fig. 1, Table 2). We report that a panel of endothelial and angiogenic biomarkers is able to discriminate, with a high degree of accuracy, children with retinopathy-confirmed CM (CM-R) from those with uncomplicated infection (UM) or with non-malarial febrile illness and altered consciousness (CNS controls) (Fig. 2. Table 3&4). This is the first study to demonstrate the specificity of Ang-1 for CM in children, and we confirm and extend previous observations of elevated levels of VWFpp and VWF in children with severe malaria compared to non-malarial febrile illness . Further, we show a marked and uniform decrease in Ang-2:Ang-1 at follow-up (Fig. 3), suggesting that the ratio between these two proteins may offer an approach to monitor clinical response. This study represents a significant extension to previous studies of endothelial biomarkers in malaria as it includes a retinopathy-confirmed group of children with cerebral malaria and also includes children with decreased consciousness due to other febrile illnesses. Finally, we are able to go beyond description to examine different putative causal biomarkers of endothelial activation and postulate on their role in disease pathogenesis and clinical recovery.
The endothelium is a dynamic organ system representing the interface between the vascular space and vital organs. The regulation of endothelial integrity is of critical importance, particularly in the face of infection-related injury. Endothelial adhesion of parasitized red cells and endothelial activation are prominent features in the pathology of fatal malaria. Parasitized erythrocytes bind to the endothelium directly through endothelial receptors and may indirectly bind through VWF and platelet complexes . There is evidence to suggest that blood-brain-barrier dysfunction and breakdown occurs in paediatric CM –. However, the pathophysiology of CM is poorly understood. A detailed understanding of endothelial activation and regulation during infection may provide new insights into the molecular basis of severe and fatal malaria.
Angiopoietins are critical regulators of endothelial activation and integrity. Elevated Ang-2 levels have previously been associated with severe malaria in paediatric and adult populations and strategies to block Ang-2 have been suggested as novel interventions for severe malaria , , . In this study, Ang-2 was elevated in CM-R compared to UM and CNS controls but became of borderline significance after correcting for multiple comparisons. However, inclusion of Ang-2 as a component of the Ang2:Ang-1 ratio markedly improved the specificity and positive likelihood ratio compared to Ang-1 or Ang-2 alone. Levels of Ang-2 observed in this study were lower than those reported in Ugandan children with CM . This difference is likely attributable to the present study design that excluded fatal cases, in which Ang-2 levels are highest. Ang-2 has been associated with increased disease severity ,  and increased risk of death  in Asian adults with severe malaria. Together, these data suggest that changes in Ang-2 are reflective of disease severity and mortality and may be a good surrogate endpoint for trials investigating mortality or evaluating adjunctive therapies. In the context of endothelial biology, the balance between Ang-1 and Ang-2 regulates the functional responsiveness of the endothelium. Ang-1 is synthesized by peri-endothelial cells to promote vascular quiescence under normal physiologic conditions; however, the release of Ang-2 from WP bodies can inhibit Ang-1 signalling in a dose-dependent manner, resulting in local destabilization of the endothelium . In this study, there were markedly lower Ang-1 levels at presentation in children with CM-R compared to those with UM or the CNS controls. The observed decreases in Ang-1 levels combined with increases in Ang-2 may contribute to the endothelial dysfunction observed in CM.
The functions of Ang-1 and Ang-2 are also modulated by interactions with VEGF. Under normal physiological conditions of high Ang-1 and low Ang-2, VEGF can stabilize the endothelium in an anti-apoptotic state and be neuroprotective , . However, when Ang-1 levels are low, VEGF can act on the endothelium unopposed, resulting in the upregulation of Ang-2 mRNA, exocytosis of WP bodies, increased permeability of endothelial cells and upregulation of tissue factor and ICAM-1–. This may be particularly important in the context of paediatric CM, where coagulopathy and increased tissue factor expression may occur and Ang-1 levels are low , . In this study, VEGF was elevated in children with retinopathy confirmed CM compared to children with uncomplicated malaria. These findings are in contrast to reports examining VEGF in severe malaria in adults from Southeast Asia, which have reported a decrease in VEGF in fatal CM cases  and decreased VEGF associated with increased disease severity . These studies postulate that VEGF may be neuroprotective and associated with wound healing , or may reflect the accumulation of VEGF within the parasitophorous vacuole , . Other reports from children have shown no difference in CSF levels of VEGF between deaths due to malaria vs. those attributed to other causes . However, a study of Kenyan children with CM showed plasma VEGF levels were positively correlated with TNF and inversely correlated with a neuroprotective agent, erythropoietin, and high levels of plasma VEGF were associated with an increased risk of seizures, raised intracranial pressure, and papilloedema . These differences may be due to geographic location (Africa vs. Asia) or patient population (pediatric vs. adult).
Other studies in pediatric populations also suggest that VEGF is associated with disease severity. A study looking at VEGF levels in children with sepsis and meningococcemia reported the highest VEGF levels in patients with septic shock  and elevated VEGF was a useful prognostic indicator in Kawasaki disease, an acute febrile vasculitis in children . In our study, despite being elevated in CM-R, VEGF was not a particularly informative marker, as it was variable between admission and follow up in children with CM, and was non-specifically elevated in the CNS controls. However, VEGF may be playing an under-appreciated role in endothelial regulation based on the presence or absence of Ang-1 or Ang-2 in the local milieu. Consequently, it may be premature to rule out VEGF as a mediator of severe malaria, given its diverse functions. It is worth noting that VEGF is contained primarily within platelets, and plasma levels can be affected by differences in sample processing (anti-coagulant used, centrifugation time and speed, etc.). Together, our VEGF data supports the idea that VEGF may be associated with disease severity and neurologic complications in CM; however, due to its local action, future studies are warranted to further elucidate the role of VEGF in disease severity.
ICAM-1 is upregulated in the cerebral endothelium during malaria infection and is associated with parasite sequestration within the cerebral vasculature, a pathological hallmark of CM . sICAM-1 is released by activated endothelium during malaria and has been reported as a biomarker of disease severity , . Similarly, in our study, sICAM-1 was able to discriminate between UM and CM, but it was also elevated in the CNS control group. In this population, IP-10 was non-significantly elevated in retinopathy-confirmed CM and displayed poor performance as a biomarker. Previous reports have identified IP-10 as a good prognostic biomarker for malaria mortality ; however, this could not be assessed in the current study where fatal cases were excluded. Recent reports have described increased circulating concentrations of VWF and activation of the coagulation system in severe malaria, with possible implications for pathogenesis , , . It has been postulated that an increase in ultra large VWF strings and a decrease in its cleavage protein ADAMTS13 may result in platelet accumulation and contribute to sequestration of parasitized erythrocytes . In the current study, both VWF and VWFpp were elevated in CM-R compared to UM or CNS controls, suggesting that these proteins are also good candidate biomarkers for CM.
The endothelial proteins measured in this study returned to a normal range in association with clinical recovery, suggesting that the alterations in biomarker levels at presentation were mediated by the infection status of the child rather than a natural host-mediated susceptibility as a result of genetic or epigenetic changes in the biomarkers examined. In previous studies, VWFpp was shown to return to baseline three days after the initiation of anti-malarial therapy , and Ang-2 and the RH-PAT index, a measure of peripheral endothelial dysfunction, returned to normal four days post treatment .
In summary, our study is the first to examine a panel of endothelial-based biomarkers in a well characterized patient population. Recent investigations have moved towards the inclusion of non-malaria febrile illness (NMFI) as a control group to investigate the specificity of markers for malaria infection. In this study, we included a NMFI group with decreased consciousness, which approximates the clinically ideal control group, i.e. children with incidental parasitemia and coma (bacterial meningitis, viral encephalopathy, etc.). CM is a clinical definition based on the presence of malaria parasitemia and coma with no other identifiable cause. However, in malaria endemic areas, there are often resource constraints that make exclusion of alternative causes of coma problematic.
The results of this study are encouraging, and suggest that a limited panel of endothelial biomarkers may be useful in differentiating between retinopathy positive CM and non-malarial febrile illness with decreased consciousness. However, these results will need to be confirmed in prospective studies and assessed alongside other infectious causes of coma in paediatric populations. Further validation of biomarkers needs to be performed to determine whether these markers: i) are informative in stratifying subjects in clinical trials or pathogenesis studies; ii) may have clinical utility in the diagnosis of true CM, assessment of disease severity and response to therapy (allocation of resources), or to determine prognosis; and iii) represent novel therapeutic targets for adjunctive therapy.
Levels of plasma biomarkers at admission and one month follow up visit from retinopathy positive children with CM.
We would like to thank all the parents, guardians and patients for participating as well as all the clinicians, nurses, and study team for their efforts.
Conceived and designed the experiments: ALC TET MEM KCK. Performed the experiments: ALC. Analyzed the data: ALC MH. Contributed reagents/materials/analysis tools: SG KBS MM TET MEM KCK. Wrote the paper: ALC KCK. Contributed to revisions of the text: ALC HP MH SG MM KBS TET MEM KCK. Organized collection of clinical samples and clinical data: HP SG KBS MM TET MEM.
- 1. Taylor TE, Fu WJ, Carr RA, Whitten RO, Mueller JS, et al. (2004) Differentiating the pathologies of cerebral malaria by postmortem parasite counts. Nat Med 10: 143–145.
- 2. Berkley JA, Mwangi I, Mellington F, Mwarumba S, Marsh K (1999) Cerebral malaria versus bacterial meningitis in children with impaired consciousness. QJM 92: 151–157.
- 3. Okubadejo NU, Danesi MA (2004) Diagnostic issues in cerebral malaria: a study of 112 adolescents and adults in Lagos, Nigeria. Niger Postgrad Med J 11: 10–14.
- 4. Wright PW, Avery WG, Ardill WD, McLarty JW (1993) Initial clinical assessment of the comatose patient: cerebral malaria vs. meningitis. Pediatr Infect Dis J 12: 37–41.
- 5. World Health Organization (2000) Severe and complicated malaria. Trans R Soc Trop Med Hyg 94: Suppl1–90.
- 6. Medana IM, Turner GD (2006) Human cerebral malaria and the blood-brain barrier. Int J Parasitol 36: 555–568.
- 7. Jakobsen PH, Morris-Jones S, Ronn A, Hviid L, Theander TG, et al. (1994) Increased plasma concentrations of sICAM-1, sVCAM-1 and sELAM-1 in patients with Plasmodium falciparum or P. vivax malaria and association with disease severity. Immunology 83: 665–669.
- 8. Hollestelle MJ, Donkor C, Mantey EA, Chakravorty SJ, Craig A, et al. (2006) von Willebrand factor propeptide in malaria: evidence of acute endothelial cell activation. Br J Haematol 133: 562–569.
- 9. Larkin D, de Laat B, Jenkins PV, Bunn J, Craig AG, et al. (2009) Severe Plasmodium falciparum malaria is associated with circulating ultra-large von Willebrand multimers and ADAMTS13 inhibition. PLoS Pathog 5: e1000349.
- 10. Yeo TW, Lampah DA, Gitawati R, Tjitra E, Kenangalem E, et al. (2008) Angiopoietin-2 is associated with decreased endothelial nitric oxide and poor clinical outcome in severe falciparum malaria. Proc Natl Acad Sci U S A 105: 17097–17102.
- 11. Findley CM, Cudmore MJ, Ahmed A, Kontos CD (2007) VEGF induces Tie2 shedding via a phosphoinositide 3-kinase/Akt dependent pathway to modulate Tie2 signaling. Arterioscler Thromb Vasc Biol 27: 2619–2626.
- 12. Fiedler U, Augustin HG (2006) Angiopoietins: a link between angiogenesis and inflammation. Trends in Immunology 27: 552–558.
- 13. Fiedler U, Reiss Y, Scharpfenecker M, Grunow V, Koidl S, et al. (2006) Angiopoietin-2 sensitizes endothelial cells to TNF-alpha and has a crucial role in the induction of inflammation. Nat Med 12: 235–239.
- 14. Lobov IB, Brooks PC, Lang RA (2002) Angiopoietin-2 displays VEGF-dependent modulation of capillary structure and endothelial cell survival in vivo. Proc Natl Acad Sci U S A 99: 11205–11210.
- 15. Sadler JE (1998) Biochemistry and genetics of von Willebrand factor. Annu Rev Biochem 67: 395–424.
- 16. Tchinda VHM, Tadem AD, Tako EA, Tene G, Fogako J, et al. (2007) Severe malaria in Cameroonian children: correlation between plasma levels of three soluble inducible adhesion molecules and TNF-[alpha]. Acta Tropica 102: 20–28.
- 17. Turner GD, Morrison H, Jones M, Davis TM, Looareesuwan S, et al. (1994) An immunohistochemical study of the pathology of fatal malaria. Evidence for widespread endothelial activation and a potential role for intercellular adhesion molecule-1 in cerebral sequestration. Am J Pathol 145: 1057–1069.
- 18. Armah H, Wilson N, Sarfo B, Powell M, Bond V, et al. (2007) Cerebrospinal fluid and serum biomarkers of cerebral malaria mortality in Ghanaian children. Malaria Journal 6: 147.
- 19. Jain V, Armah H, Tongren J, Ned R, Wilson N, et al. (2008) Plasma IP-10, apoptotic and angiogenic factors associated with fatal cerebral malaria in India. Malaria Journal 7: 83.
- 20. Beare NA, Taylor TE, Harding SP, Lewallen S, Molyneux ME (2006) Malarial retinopathy: a newly established diagnostic sign in severe malaria. Am J Trop Med Hyg 75: 790–797.
- 21. Lewallen S, Bronzan RN, Beare NA, Harding SP, Molyneux ME, et al. (2008) Using malarial retinopathy to improve the classification of children with cerebral malaria. Trans R Soc Trop Med Hyg 102: 1089–1094.
- 22. Burton M, Nyong'o O, Burton K, John W, Inkoom E, et al. (2004) Retinopathy in Gambian children admitted to hospital with malaria. Trop Doct 34: 214–218.
- 23. Bridges DJ, Bunn J, van Mourik JA, Grau G, Preston RJS, et al. (2009) Rapid activation of endothelial cells enables P. falciparum adhesion to platelet decorated von Willebrand factor strings. Blood: blood-2009-2007-235150.
- 24. Brown H, Hien TT, Day N, Mai NT, Chuong LV, et al. (1999) Evidence of blood-brain barrier dysfunction in human cerebral malaria. Neuropathol Appl Neurobiol 25: 331–340.
- 25. Brown H, Rogerson S, Taylor T, Tembo M, Mwenechanya J, et al. (2001) Blood-brain barrier function in cerebral malaria in Malawian children. Am J Trop Med Hyg 64: 207–213.
- 26. van der Heyde HC, Nolan J, Combes V, Gramaglia I, Grau GE (2006) A unified hypothesis for the genesis of cerebral malaria: sequestration, inflammation and hemostasis leading to microcirculatory dysfunction. Trends Parasitol 22: 503–508.
- 27. Lovegrove FE, Tangpukdee N, Opoka RO, Lafferty EI, Rajwans N, et al. (2009) Serum Angiopoietin-1 and -2 Levels Discriminate Cerebral Malaria from Uncomplicated Malaria and Predict Clinical Outcome in African Children. PLoS ONE 4: e4912.
- 28. Conroy AL, Lafferty EI, Lovegrove FE, Krudsood S, Tangpukdee N, et al. (2009) Whole blood angiopoietin-1 and -2 levels discriminate cerebral and severe (non-cerebral) malaria from uncomplicated malaria. Malar J 8: 295.
- 29. Yuan HT, Khankin EV, Karumanchi SA, Parikh SM (2009) Angiopoietin 2 is a Partial Agonist/Antagonist of Tie 2 Signalling in Endothelium. Mol Cell Biol: MCB.01472–01408.
- 30. Nicoletti JN, Shah SK, McCloskey DP, Goodman JH, Elkady A, et al. (2008) Vascular endothelial growth factor is up-regulated after status epilepticus and protects against seizure-induced neuronal loss in hippocampus. Neuroscience 151: 232–241.
- 31. Carmeliet P, Storkebaum E (2002) Vascular and neuronal effects of VEGF in the nervous system: implications for neurological disorders. Semin Cell Dev Biol 13: 39–53.
- 32. Oh H, Takagi H, Suzuma K, Otani A, Matsumura M, et al. (1999) Hypoxia and vascular endothelial growth factor selectively up-regulate angiopoietin-2 in bovine microvascular endothelial cells. J Biol Chem 274: 15732–15739.
- 33. Matsushita K, Yamakuchi M, Morrell CN, Ozaki M, O'Rourke B, et al. (2005) Vascular endothelial growth factor regulation of Weibel-Palade-body exocytosis. Blood 105: 207–214.
- 34. Satchell SC, Anderson KL, Mathieson PW (2004) Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties. J Am Soc Nephrol 15: 566–574.
- 35. Kim I, Oh JL, Ryu YS, So JN, Sessa WC, et al. (2002) Angiopoietin-1 negatively regulates expression and activity of tissue factor in endothelial cells. Faseb J 16: 126–128.
- 36. Kim I, Moon SO, Park SK, Chae SW, Koh GY (2001) Angiopoietin-1 reduces VEGF-stimulated leukocyte adhesion to endothelial cells by reducing ICAM-1, VCAM-1, and E-selectin expression. Circ Res 89: 477–479.
- 37. Moxon CA, Heyderman RS, Wassmer SC (2009) Dysregulation of coagulation in cerebral malaria. Mol Biochem Parasitol 166: 99–108.
- 38. Francischetti IM, Seydel KB, Monteiro RQ, Whitten RO, Erexson CR, et al. (2007) Plasmodium falciparum-infected erythrocytes induce tissue factor expression in endothelial cells and support the assembly of multimolecular coagulation complexes. J Thromb Haemost 5: 155–165.
- 39. Sachanonta N, Medana IM, Roberts R, Jones M, Day NP, et al. (2008) Host vascular endothelial growth factor is trophic for Plasmodium falciparum-infected red blood cells. Asian Pac J Allergy Immunol 26: 37–45.
- 40. Casals-Pascual C, Idro R, Gicheru N, Gwer S, Kitsao B, et al. (2008) High levels of erythropoietin are associated with protection against neurological sequelae in African children with cerebral malaria. Proc Natl Acad Sci U S A 105: 2634–2639.
- 41. Pickkers P, Sprong T, Eijk L, Hoeven H, Smits P, et al. (2005) Vascular endothelial growth factor is increased during the first 48 hours of human septic shock and correlates with vascular permeability. Shock 24: 508–512.
- 42. Kentaro U, Yuichi N, Teruto H, Kiminori M, Yasuko M, et al. (2009) Platelet vascular endothelial growth factor is a useful predictor for prognosis in Kawasaki syndrome. British Journal of Haematology 148: 285–292.
- 43. Turner GD, Ly VC, Nguyen TH, Tran TH, Nguyen HP, et al. (1998) Systemic endothelial activation occurs in both mild and severe malaria. Correlating dermal microvascular endothelial cell phenotype and soluble cell adhesion molecules with disease severity. Am J Pathol 152: 1477–1487.