Thiamine (vitamin B1) is an essential molecule for all life forms because thiamine diphosphate (ThDP) is an indispensable cofactor for oxidative energy metabolism. The less abundant thiamine monophosphate (ThMP), thiamine triphosphate (ThTP) and adenosine thiamine triphosphate (AThTP), present in many organisms, may have still unidentified physiological functions. Diseases linked to thiamine deficiency (polyneuritis, Wernicke-Korsakoff syndrome) remain frequent among alcohol abusers and other risk populations. This is the first comprehensive study on the distribution of thiamine derivatives in human biopsies, body fluids and cell lines.
Methodology and Principal Findings
Thiamine derivatives were determined by HPLC. In human tissues, the total thiamine content is lower than in other animal species. ThDP is the major thiamine compound and tissue levels decrease at high age. In semen, ThDP content correlates with the concentration of spermatozoa but not with their motility. The proportion of ThTP is higher in humans than in rodents, probably because of a lower 25-kDa ThTPase activity. The expression and activity of this enzyme seems to correlate with the degree of cell differentiation. ThTP was present in nearly all brain and muscle samples and in ∼60% of other tissue samples, in particular fetal tissue and cultured cells. A low ([ThTP]+[ThMP])/([Thiamine]+[ThMP]) ratio was found in cardiovascular tissues of patients with cardiac insufficiency. AThTP was detected only sporadically in adult tissues but was found more consistently in fetal tissues and cell lines.
Conclusions and Significance
The high sensitivity of humans to thiamine deficiency is probably linked to low circulating thiamine concentrations and low ThDP tissue contents. ThTP levels are relatively high in many human tissues, as a result of low expression of the 25-kDa ThTPase. Another novel finding is the presence of ThTP and AThTP in poorly differentiated fast-growing cells, suggesting a hitherto unsuspected link between these compounds and cell division or differentiation.
Citation: Gangolf M, Czerniecki J, Radermecker M, Detry O, Nisolle M, Jouan C, et al. (2010) Thiamine Status in Humans and Content of Phosphorylated Thiamine Derivatives in Biopsies and Cultured Cells. PLoS ONE 5(10): e13616. https://doi.org/10.1371/journal.pone.0013616
Editor: German Malaga, Universidad Peruana Cayetano Heredia, Peru
Received: June 21, 2010; Accepted: September 30, 2010; Published: October 25, 2010
Copyright: © 2010 Gangolf et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: LB is Research Director, BL is Research Associate and is MG Research Fellow at the "Fonds de la Recherche Scientifique-FNRS". The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Thiamine is an essential molecule for all life forms. In animal cells, thiamine is phosphorylated to ThDP by a specific enzyme, thiamine pyro(di)phosphokinase (TPK, EC 184.108.40.206) (Fig. 1). As ThDP is a cofactor for transketolase and pyruvate and 2-oxoglutarate dehydrogenase complexes required for the oxidative degradation of sugars and mitochondrial synthesis of ATP, thiamine deficiency results in acute energy failure. In contrast to microorganisms and plants, which are able to synthesize thiamine de novo, animals require an exogenous source. Thiamine deficiency in humans causes beriberi, a peripheral neuropathy and, mostly in alcohol-misusing patients, Wernicke-Korsakoff syndrome , . Based on postmortem analysis, the prevalence of the latter is 1–2% of the general population and 12–14% of the misusing population , making it the third commonest cause of dementia, after Alzheimer's disease and vascular dementias . Marginal thiamine deficiency is probably more common than thought in the elderly , , , , in risk groups such as HIV-positive patients , , , in patients with fast-growing hematologic malignant tumors , after chronic liver failure  or following gastrectomy . Accidental thiamine deficiency was also recently documented by the 2003 outbreak of encephalopathy in infants in Israel, caused by a defective soy-based formula .
1, cytosolic thiamine diphosphokinase (TPK); 2, cytosolic thiamine diphosphatase (also hydrolyzes nucleoside diphosphates); 3, thiamine monophosphatase (hypothetical); 4, mitochondrial membrane-associated ThTP synthase; 5, cytosolic adenylate kinase; 6, cytosolic 25-kDa ThTPase; 7, cytosolic ThDP adenylyl transferase; 8, AThTP hydrolase (hypothetical). The mechanisms of AThDP synthesis and degradation are unknown. Δp, transmembrane H+ gradient. Updated from .
Thiamine deficiency or defects in thiamine metabolism were also reported in other human pathologies. While one study reported normal total blood thiamine levels in patients with Alzheimer's dementia , others showed decreased levels in plasma or erythrocytes , . ThDP levels are decreased in postmortem brains of patients with Alzheimer's disease ,  and frontal lobe degeneration of the non-Alzheimer's type .
Thiamine deficiency induces neuronal loss and cardiac failure. The brain and the heart have an absolute requirement for oxidative metabolism, and it is generally thought that the clinical symptoms observed during thiamine deficiency result from decreased tissular ThDP levels. However, in addition to the well-known cofactor ThDP, other thiamine derivatives might also play physiological roles  (Fig. 1). ThTP exists in animal tissues in variable amounts . ThTP is able to phosphorylate proteins  and to activate large conductance anion channels in excised patches of mouse neuroblastoma cells , but the physiological significance of these results remains to be proven.
The mechanism of ThTP synthesis has long been controversial. It now appears that there are at least two different mechanisms. Kawasaki and coworkers showed that adenylate kinase 1 (AK1) is able to synthesize ThTP according to the reaction ThDP + ADP ThTP + AMP , . This reaction may lead to a significant synthesis of ThTP in skeletal muscle where this enzyme is particularly abundant. We have shown that synthesis of ThTP is a feature of all known AKs and that this reaction is always 106–107 times slower than ATP synthesis according to the reaction 2 ADP ATP + AMP . In brain however, ThTP is synthesized in mitochondria by a chemiosmotic mechanism, according to the reaction ThDP + Pi ThTP coupled to the respiratory chain .
The enzymatic hydrolysis of ThTP has been reported in many tissues. Mammalian tissues contain a membrane-associated ThTPase , ,  and a soluble cytosolic ThTPase . The membrane-associated ThTPase has not been characterized at the molecular level. The soluble ThTPase (EC 220.127.116.11) is a 25-kDa protein belonging to the CYTH superfamily  or triphosphate tunnel metalloenzymes  and its three-dimensional structure is known . This ThTPase is expressed in virtually all mammalian tissues , ,  and appears to be mainly neuronal in brain . Orthologs of the 25-kDa ThTPase are present in all animal species, except possibly in birds .
Recently, we discovered the first thiamine adenine nucleotide, adenosine thiamine triphosphate (AThTP), which accumulates in E. coli during carbon starvation or collapse of the membrane H+ gradient , . AThTP is also present in mammalian tissues. In E. coli it can be synthesized by a ThDP adenylyl transferase, present in the cytosol , according to the reaction ThDP + ADP AThTP + Pi. In carbon-starved E. coli, it rapidly disappears after addition of glucose , suggesting the presence of an AThTP-hydrolyzing enzyme. Furthermore, another adenine thiamine nucleotide, adenosine thiamine diphosphate (AThDP), exists, at least in mouse and quail liver , but we no information concerning its synthesis or degradation.
We only begin to understand the metabolism of thiamine derivatives and in particular ThTP and AThTP. There are only a few studies on the distribution of these compounds in animal tissues ,  and only two (from the same group) in humans showing decreased ThTP levels in the postmortem brains of patients with subacute necrotizing encephalomyelopathy (Leigh's disease) , . However, the compound measured in the latter study may not have been authentic ThTP . Indeed, ThTP measurements were unreliable before the development of HPLC techniques and we were unable to detect ThTP in human postmortem brains , , probably because of hydrolysis during the postmortem delay.
Therefore, it is important to obtain information on the thiamine status in humans, which can only be done reliably using fresh tissue samples, especially in the case of ThTP and AThTP, which are subject to rapid metabolic degradation. This is the first study on the content of thiamine derivatives in various biopsied human tissue samples and some human cultured cells. We also checked the expression of the 25-kDa ThTPase by determination of its enzyme activity, immunoblotting and RT-PCR. The results obtained allow us to draw several interesting conclusions concerning the distribution and the abundance of thiamine compounds in humans.
Biological stability and turnover of thiamine derivatives in biological samples
A general problem associated with the detection of metabolites in tissues is related to their biological stability. ATP, for instance has a very high turnover with a half-life <1 s. Thus, even the short delay between the sacrifice of the animal and the freezing of the target organ is sufficient for most of the ATP to be hydrolyzed , . Reliable estimations of cellular ATP concentrations are therefore difficult to obtain. The turnover of thiamine derivatives is much slower, so that this issue is less important. We previously showed that in cultured mouse neuro-2a cells, the turnover time of ThDP is ∼17 h at 37°C . In a previous study on human brain samples, we did not find a significant effect of the postmortem delay (3–27 h, n = 59) on ThDP levels , suggesting that reliable estimations of ThDP content can be obtained on human postmortem samples. However, it should be kept in mind that a hydrolysis of only few percent of ThDP during the postmortem delay would often go unnoticed but would lead to an important increase in the comparatively minor ThMP, the product of hydrolysis of ThDP. In the case of ThTP, the turnover time is ∼1 h in neuro-2a cells which is slow enough for no significant hydrolysis to occur during a normal sampling procedure. However, a strong underestimation would be obtained for longer postmortem delays. Indeed, in previous studies, we were unable to detect any ThTP in human brain postmortem samples , . Therefore, a reliable estimation of ThTP content can only be obtained from human biopsies. No data are available concerning the biological stability of adenylated thiamine compounds in animal tissues, but in carbon-starved E. coli, AThTP disappears within a few minutes after addition of glucose .
Thiamine compounds in human blood and cerebrospinal fluid (CSF)
Thiamine phosphate esters are hydrolyzed in the intestinal lumen by various phosphatases (mainly the alkaline phosphatase associated with brush-border membranes) and thiamine is taken up through the intestinal mucosal membrane by a specific saturable transporter (Km = 4.4 µM) through an energy-dependent process , . The vitamin is transported into erythrocytes by another saturable, high-affinity transporter (Km = 0.11 µM) and a non-saturable component  that is probably not relevant at physiological plasma concentrations. Indeed, the total (free + protein-bound) thiamine concentration in human plasma is very low (10–20 nM), while it reaches over 100 nM in rodent plasma, which in addition also contains high amounts of ThMP . Inside the erythrocytes, thiamine is pyrophosphorylated to ThDP by TPK according to the reaction Thiamine + ATP ThDP + AMP. Though the equilibrium constant for this reaction is not in favor of ThDP formation , the steady-state ThDP concentration in erythrocytes is much higher than that of free thiamine. It is known that ThDP binds to transketolase with high affinity, but the molar concentration of the enzyme is probably too low to explain the shift of the equilibrium towards ThDP synthesis. Another explanation is that the AMP formed is converted to ADP by AK1, which is abundant in human red blood cells . This would explain that the free ThDP can accumulate to some extent in the cytoplasm and is trapped in the cell (as it cannot cross the membrane). In human erythrocytes, up to 10% of the free ThDP can be converted to ThTP, a reaction catalyzed (albeit very slowly) by AK1 according to the reaction ThDP + ADP ThTP + AMP .
Many different methods for the determination of thiamine derivatives in human whole blood, erythrocytes, serum or plasma have been described and it is not the aim of the present work to review all these data, which has been done previously , . Here, we essentially wanted to investigate the possible presence of ThTP and AThTP in these fluids.
In agreement with previous results , , , , whole human blood contained thiamine, ThMP, and ThDP (Table 1). ThTP accounted for nearly 10% of total thiamine, which is more than in most human cells, while ThMP and free thiamine were less abundant. We detected no significant amounts of AThDP or AThTP in human blood. Plasma contained low amounts of thiamine and ThMP, but neither ThDP nor ThTP. Similar concentrations of free thiamine (10–20 nmol/L) in human plasma or serum have been reported by us  and other workers , , . In human blood, total thiamine concentrations are nearly an order of magnitude lower than in blood of rodents and other animals , .
In human CSF, we only found thiamine and ThMP, in agreement with Rindi and coworkers . ThMP is probably transported through the choroid plexus by the reduced folate transporter (SLC19A1), that is very abundant in the apical (CSF) side of the choroid plexus  and able to transport ThMP (but not thiamine) in addition to reduced folate .
Thiamine compounds in various tissues including the cardiovascular system
We determined thiamine compounds in biopsies from various human tissues, including colon, lung, thymus, skin, skeletal muscle and various elements of the cardiovascular system from 33 patients (Table 2). In some cases, several kinds of anatomically different samples from similar tissues (veins, arteries or valves) were pooled for statistical reasons, as there were no obvious differences between them. The 33 patients were aged between 37 and 82 years. At first, it appeared that the content of thiamine compounds was highly variable from one patient to another, as evidenced by the high coefficient of variation (Cv), often >0.7. ThMP and ThDP were found in all patients. Free thiamine and ThTP were found in only half of the samples. Skeletal muscle always contained measurable amounts of ThTP, except for one patient aged 82 with also very low ThDP levels. The presence of ThTP (mostly cytosolic) in skeletal muscle is probably related to the high content of AK1. As mentioned above, erythrocytes contain relatively high amounts of ThTP for probably the same reason.
In most tissues investigated, the cofactor ThDP was the most abundant thiamine compound, with the highest content in heart auricles and skin, followed by kidney, lung, colon, adipose tissue, skeletal muscle and vascular samples. We tested whether there was a correlation between the age of the patients and the content of thiamine derivatives. Indeed, using linear regression analysis, we found a significant negative correlation between age and ThDP levels. The ThDP content decreased with the age of the patients in skeletal muscle [F(1,9) = 6.701, p = 0.0293, R2 = 0.43] and lung [F(1,5) = 14,730, p = 0,0121, R2 = 0.75], for which we had the most complete series of samples. No significant correlation was found for ThTP [F(1,8) = 0.6348, p = 0.45, R2 = 0.074] or ThMP [F(1,9) = 1.547, p = 0.25, R2 = 0.15] in skeletal muscle, but a significant negative correlation between ThMP and age [F(1,5) = 11.853, p = 0.0184, R2 = 0.70] was observed in lung. A significant negative correlation was also found between total thiamine content and age in skeletal muscle [F(1,9) = 9.408, p = 0.0134, R2 = 0.51] and lung [F(1,5) = 17.6073, p = 0.0085, R2 = 0.78]. In a previous study limited to human postmortem brains, we already observed a tendency for decreased ThDP and total thiamine levels with age, and in particular in the highest age group (≥77 years) , though TPK activities were normal.
In veins and arteries, ThDP levels were relatively low, while ThTP levels were proportionally high, accounting in two patients for about 15%–16% of total thiamine. This seems to be a characteristic of human tissues, as in aorta and vena cava from rats, ThTP represented only respectively 0.6% (0.27 pmol/mg of protein, n = 3) and 0.3% (0.23 pmol/mg of protein, n = 3) of total thiamine (not shown).
It is interesting to note that, in some patients, ThTP was below detection limit in all tissues, except skeletal muscle. In these patients, free thiamine was present, sometimes in high amounts. Therefore, we separated the patients in two categories: those with a “positive” thiamine phosphorylation shift (toward ThTP) and those with a “negative” thiamine phosphorylation shift (toward thiamine). We calculated the ([ThTP] + [ThMP])/([Thiamine] + [ThMP]) ratio for each patient by taking the mean of all tissues from the same patient. We have previously shown that there are at least two ThDP pools, a larger low-turnover cofactor pool and a smaller high-turnover pool, precursor of ThTP and ThMP , . Therefore, as we do not know the size of the free high-turnover ThDP pool, this derivative was omitted from the equation. We called this ratio the “thiamine phosphorylation ratio” (TPR). 20 patients (61%) had a TPR ≥1, while 11 (39%) had a TPR <1. We did not find a significant correlation between TPR and total thiamine content in our samples [F(1,28) = 0.0059, p = 0.94, R2 <0.0001]. Also, the TPR did not correlate with the age of the patients [F(1,28) = 0,09719, p = 0,7575, R2 = 0,003459].
From the retrospective analysis of the clinical charts, we did not find arguments for a relation between underlying neoplastic disease and a low TPR. However, among the patients with low TPR all but one had a significant degree of heart failure based on clinical assessment (New York Heart Association Functional Classification) and echocardiographic data. The heart failure syndrome is characterized by a diffuse neuro-hormonal activation and metabolic shifts , . Therefore, this possible and plausible correlation warrants further study and is currently under prospective investigation in our institution.
As for AThTP, it was detected in only 15 samples from 10 patients (out of 33). AThTP was present in 35% of the samples containing AThTP, but in only 3% of the samples devoid of ThTP. This difference is highly significant (p = 0.0009), suggesting that the appearance of AThTP is linked to the presence of ThTP. This is in contrast to what is observed in bacteria where ThTP and AThTP do not generally accumulate together .
Thiamine derivatives in normal and ischemic human liver
We thought that ischemia might affect the distribution of thiamine derivatives in liver tissue. Indeed, it is known that thiamine protects against hypoxia-induced cell death in cultured neonatal rat cardiomyocytes  and accelerates the healing of diabetic ischemic limbs in mice . This may be one of the reasons why thiamine supplementation improves renal transplantation outcome in humans . However, we did not observe any effect of ischemia on the levels of thiamine compounds in liver (Table 3). Neither AThDP nor AThTP were found in human liver, in contrast to mouse  or rat  liver. Human liver contained significant amounts of ThTP (>1% of total thiamine), and ∼73% of ThTP was found in the supernatant after centrifugation of the liver homogenate at 100 000× g. This may be a difference with brain or cardiac tissue, where a large part of ThTP is mitochondrial  and might therefore be more sensitive to ischemia.
Thiamine compounds and 25-kDa ThTPase in gynecological and embryonic tissues
As the fetus continuously grows, it acts as a thiamine sink, explaining a 10 to 1 ratio of the thiamine concentrations in the maternal versus venous cord plasma . ThDP was the major compound observed in human adult gynecological specimen and in embryonic tissues (Table 4). A high amount of ThMP was also found in several samples such as fibroma, ligament, serosa and endometrium. It could be argued that this high proportion of ThMP is the result of a hydrolysis of ThDP after the sample was taken. However, we took care, first, to freeze the samples as quickly as possible and the delay was never longer than a few minutes (generally less than 2 minutes). Secondly, we previously showed that, at least in the human brain, a postmortem delay of several hours does not significantly change the relative thiamine, ThMP or ThDP levels , , suggesting that ThDP hydrolysis is slow. Indeed, though some nucleoside diphosphatases are able to hydrolyze ThDP , , no specific thiamine diphosphatase has yet been characterized. This does however not exclude that in some tissues unspecific phosphatases might be responsible for a rapid hydrolysis of ThDP. This is in contrast to ThTP, which rapidly disappears during the postmortem delay, probably because of the action of the specific 25-kDa ThTPase or mitochondrial phosphohydrolases .
ThTP was consistently found in fetal tissue and in particular placenta, but only sporadically in adult tissues. This also holds, though to a lesser extent, for AThTP. Indeed, among the samples containing ThTP, 53% also contained AThTP, while AThTP was only found in 10% of the samples devoid of ThTP (p = 0.0006). Therefore, as for the samples of Table 2, these data suggest that the presence of AThTP is linked to the presence of ThTP. AThDP was only rarely detected.
We compared the activities of the 25-kDa ThTPase in these tissues (Table 5). The enzyme activity was low in fetus and trophoblast, but it was substantially higher in endometrium and uterus. A high activity was also previously observed in mouse uterus  and a high expression of the ThTPase mRNA in human uterus , in agreement with our present data. While significant amounts of ThTP are observed in fetal tissues, this compound is below detection limit in adult gynecological tissues (Table 4). These results suggest that 25-kDa ThTPase is important for the regulation of cellular ThTP levels in vivo, which has never been demonstrated before.
Thiamine compounds in human semen
Spermatogenesis requires thiamine, as evidenced in transgenic mice lacking the high-affinity thiamine carrier ThTR1 . Null males have hypoplastic testes secondary to apoptosis of pachytene stage spermatocytes, but to our knowledge, no determination of thiamine compounds in human semen has ever been published. Our results show that whole semen contained thiamine, ThMP and ThDP in variable amounts (Table 6). No adenylated thiamine derivatives were found. We observed a positive correlation [F(1,10) = 21.36, p<0.0009, R2 = 0.68] between ThDP content and number of spermatozoa per mL of semen, which varied from 1.8.106 to 135.106 (azoospermic semen samples were not included in this correlation). No significant correlations were found for thiamine or ThMP. These results suggest that ThDP is mainly localized within spermatozoa. This conclusion is also supported by the observation that ThDP concentration was low in seminal fluid prepared from whole semen. Several studies suggested a relationship between sperm motility and oxidative phosphorylation , while other studies suggested that, at least in human sperm cells, glycolysis is a primary energy source for motility . We did not find a significant correlation [F(1,10) = 3.890, p = 0.0768, R2 = 0.28]) between ThDP content of spermatozoa and the percentage of motility (% of spermatozoa with a progressive motility ≥25 µm/sec at 37°C or ≥20 µm/sec at 20°C), though a tendency seemed to exist. ThDP is a cofactor for transketolase in the pentose phosphate shunt and it was shown that experimental inhibition of the pentose phosphate pathway impaired progressive motility of human spermatozoa . It is therefore plausible that the tendency of highly motile spermatozoa to have a high ThDP content is indicative of the functioning of the pentose phosphate pathway rather than of mitochondrial metabolism. Indeed, the pentose phosphate pathway is the main source for NADPH, required for the reduction of glutathione by glutathione reductase. Reduced glutathione is essential in antioxidant defense for spermatozoa, which have limited antioxidant capacity because of their small cytoplasmic volume. ThDP, a cofactor for transketolase, is essential for maintaining the flux through the oxidative part of the pathway, when the requirement or NADPH is high.
In some samples, a peak eluting close to ThTP was observed. It did however not disappear upon treatment with ThTPase, suggesting that it was not ThTP. Testis and prostate are among the tissues that display the highest expression of 25-kDa ThTPase mRNA , . In rat testis, 25-kDa ThTPase mRNA is highly expressed in cells close to the basal lamina of the seminiferous tubules. No signal was observed in mature spermatozoa, probably because the cytoplasmic volume is extremely reduced.
Thiamine derivatives in the brain
We determined thiamine derivatives in pig, rat, quail and human brain samples (Table 7). ThDP and thus total thiamine levels were much lower in human brain than in rat, quail or pig brain. The Cv for ThDP was 5/21 = 0.24, which was the same as the Cv for postmortem brains previously obtained  and lower than for many other tissues in this study, such as skeletal muscle (0.71, Table 2), heart auricle (0.67, Table 2) or liver (0.64, Table 3). In a previous study, we checked thiamine derivatives in the brain of the baboon Papio papio  and the mean ThDP content was ∼5 nmol/g of fresh weight. Considering that proteins account for about 13% of fresh weight, this corresponds to 38 pmol/mg of protein, a value twice higher than for human brain, but still much lower than for rodent brain.
A previous study on postmortem human brain also did not reveal important regional differences in the distribution of thiamine derivatives, though ThDP levels were somewhat higher than average in mammillary bodies and lower in hippocampus .
In human brain, ThTP levels were relatively high, accounting for ∼1% of total thiamine. Even higher ThTP levels were found in quail and pig brain in agreement with previous results . This is mainly due to the presence of very low catalytic activity of the 25-kDa ThTPase in pigs , while birds probably have no 25-kDa ThTPase . These results clearly suggest an inverse correlation between brain ThTP content and soluble 25-kDa ThTPase activity.
In rat brain, we compared brainstem, right and left cortical hemispheres as well as cerebellum. There were no important differences between the regions, except that ThTP levels were significantly higher in the brainstem than in the cerebellum (p<0.05). AThTP levels were low and highly variable. While AThTP was found in rat brain, it was always absent in pig, quail and human brain.
ThTP and AThTP in cultured human and rodent cell lines
Thiamine derivatives were determined in cultured cells from human and rodent origin. We tested human neuroblastoma cells (SK-N-BE), human glioblastoma cells (LN-18), mouse myoblasts (C2C12), mouse fibroblasts (3T3), mouse neuroblastoma (neuro-2a) and rat phaeochromocytoma (PC-12) cells (Table 8).
Significant amounts of ThTP were detected in all cell lines except 3T3. Surprisingly, AThTP was more abundant than ThTP in all cell lines except the mouse neuroblastoma neuro-2a cells. The highest amount of AThTP was found in human glioblastoma LN-18 cells. It should be reminded that AThTP was also consistently detected in tissues that proliferate quickly (placenta, trophoblast and fetus at an early state of development, Table 4). Therefore the presence of AThTP, ThTP and a low 25-kDa ThTPase activity may be features of undifferentiated fast-growing cells.
Expression of 25-kDa ThTPase in brain and cell lines from different species
As various phosphatases are able to hydrolyze ThTP, we made several tests to make sure that ThTP hydrolysis observed in extracts from cultured cells is catalyzed by 25-kDa ThTPase. We checked that the pH optimum is around 8.5, characteristic of 25-kDa ThTPase, and that the enzyme activity was observed in the presence of Mg2+ and not Ca2+ . The substrate concentration used was 10 µM as, at such low concentrations, ThTP hydrolysis by alkaline phosphatase for instance is negligible.
25-kDa ThTPase activity is significant in human brain, but ∼20 times lower than in mouse brain (Fig. 2). This might explain the relatively high proportion of ThTP compared to total thiamine in human brain, compared with other species such as rodents. Furthermore, 25-kDa ThTPase mRNA expression was uniformly low in human brain with a somewhat higher expression in the parietal and occipital lobes and a lower expression in the cerebellum . This is also true in mouse brain: both mRNA expression and enzyme activities were somewhat lower in the cerebral compared to the cerebellar cortex .
(A) ThTPase specific activities measured at pH 8.5, at 37°C and at a Mg2+ concentration of 10 mM (means ± SD, n = 3) in mouse and human brain and cell lines. (B) pH profile of ThTPase activity from various sources (means ± SD, n = 2). The buffers (50 mM final concentration) used were: Pipes (pH 6.5), Mops (pH 7.1), Hepes (pH 7.5), Taps (pH 8.0), Bis-Tris-Propane (pH 8.5) and Ches (pH 9.1 and 9.5).
While a significant ThTPase activity could be measured in mouse C2C12 and 3T3 cells, no activity was detected in human SK-N-BE and LN-18 cells. We were unable to measure any significant ThTPase activity in human cell lines. Though the Km and Vmax for purified recombinant 25-kDa ThTPase are respectively 175±21 µM and 5.9±0.2 µmol s−1 mg−1 for the human  and a little less than 100 µM and ∼10–12 µmol s−1 mg−1 for the mouse enzyme , these differences do not explain a specific activity in human brain less than 5% of that of mouse brain. Indeed, at the 10 µM substrate concentration used in this study, both enzymes have approximately the same specific activity. Therefore, our data suggest a lower expression of the enzyme in human, compared to mouse tissues. The expression was even lower in cultured cells. This result is surprising as, by RT-PCR, mRNA could be detected in all cell lines (Fig. 3).
(SL, standard ladder; S, SN-K-BE; C, C2C12; L, LN-18).
In order to test whether this is due to the absence of the protein or the presence of a catalytically inactive form of the enzyme, we performed Western blots on human tissues and on SK-N-BE and LN-18 cells using commercial mouse monoclonal and polyclonal antibodies directed against recombinant hThTPase. The monoclonal antibody proved to be useless (see File S1). The polyclonal antibody recognized a band of the correct molecular mass in endometrium and uterus (Fig. 4), but no or possibly only very faint bands of the correct molecular mass were detected in cultured human cells and trophoblast. Purified recombinant 25-kDa human ThTPase  was used as a positive control. These results show that, in agreement with enzyme activity determinations, the 25-kDa soluble ThTPase protein is mainly expressed in adult tissues. The presence of mRNA was shown by RT-PCR analysis and therefore we propose that the expression of the protein may be controlled at the level of translation or by posttranslational modification. A similar conclusion has been reached previously for mouse tissues and it was proposed that the highly conserved 200-nucleotide 3′-untranslated mRNA region might be involved in the control of translation .
The antibody was raised against human recombinant 25-kDa hThTPase. The samples were homogenized and centrifuged (20 000× g, 30 min). In the case of the biological samples, 40 µg of cytosolic protein were loaded on the gel; for hThTPase, the amount was 0.5 µg. The arrow indicates the location of 25-kDa hThTPase. (Ex, ectocervix; En, endometrium; hT, human recombinant ThTPase; L, human LN-18 glioblastoma cells; S, human SK-N-BE neuroblastoma cells; U, uterus; Tr, trophoblast).
This is the first comprehensive study on the distribution of all known thiamine derivatives in biopsied human tissues and body fluids. Though at least one study reported the distribution of thiamine derivatives in human brain , it was a postmortem study and it is known that some derivatives, in particular ThTP, are hydrolyzed during the postmortem delay. No data were available concerning the existence of ThTP and AThTP in human tissues or cultured cells. From the present study we can draw several general conclusions.
Human tissue ThDP levels are relatively low
Tissue levels of thiamine derivatives have a high inter-patient variability in peripheral tissues. This is not observed in laboratory animals and probably results from external factors, such as nutrition, disease, age, alcohol consumption or drug treatment. Furthermore, it must be emphasized that the biopsies were from patients suffering from various clinical conditions, which might be an additional factor impacting on variability. However, human brain ThDP content, while low, was less variable than in peripheral tissues, suggesting a tight regulation of thiamine homeostasis in brain. This is further emphasized by previous findings that in the thiamine-deficient rat, the rate of disappearance of thiamine is much slower in the brain than in the liver , .
Total thiamine levels (essentially ThDP, which accounts for most of total thiamine) are much lower in humans than in rodents. Furthermore, human brain total thiamine levels are also much lower than in other animals (Table 7), which could explain the particular sensitivity of the human nervous system to thiamine deficiency. As thiamine levels are already low in human whole blood and plasma, it is possible that intestinal transport of the vitamin is not very efficient in humans. In rodents, the liver contains the highest amount of total thiamine per mg of protein, essentially in the form of ThDP. This, as well as its size, makes it a thiamine store, as recently suggested . This is also underscored by the observation that end-stage liver failure results in thiamine deficiency by depletion of thiamine stores . However, in human liver, total thiamine levels are at least an order of magnitude lower than in rodent liver. Therefore, there is only a very limited storage capacity for thiamine in the human body. This might explain that marginal thiamine deficiency is relatively common in humans. It should be reminded for instance that in the Japanese Navy in the years 1878–1882, each year a third of all enlisted men became sick with beriberi . In rats, overt symptoms of thiamine deficiency occur only after brain thiamine levels drop below 20% of controls, indicating a substantial brain reserve of the vitamin . This threshold value is slightly above the human controls. In one of the rare studies of experimental thiamine depletion in humans, the subjects developed the first clinical symptoms after only two weeks on a diet containing 0.15 mg of thiamine per day . In rodents, complete absence of thiamine in the diet leads to deficiency symptoms only after 4.5 to 9 weeks , ,  and generally, the antimetabolite pyrithiamine is administered to accelerate the development of deficiency . These differences between rodents and humans could also, at least in part, be explained by differences in intestinal flora and the presence in the rodent caecum of bacteria able to synthesize thiamine de novo. The fact that thiamine levels might be close to deficiency threshold in humans is further emphasized by the observation that thiamine supplementation can lead to increased well-being , . Our results show that, at least in muscle and lung, there is a negative correlation between age and ThDP and total thiamine content. We have previously observed that, in postmortem human brain, ThDP (and total thiamine) levels tended to be highest in very young individuals and decreased in the highest age group (>77 years) . Such a decrease would be in agreement with a decreased thiamine status in elderly people , , , probably as a result of decreased intestinal absorption . Finally, there appear to be many tissue or cell-specific differences in the metabolism of thiamine and thiamine phosphate derivatives, which could explain differences in sensitivity to thiamine deficiency, as already suggested previously for cultured human cells .
ThTP is relatively abundant in human tissues
ThTP is relatively abundant in some tissues, sometimes reaching over 10% of total thiamine (Table 2). This high ThTP content is probably linked to a relatively low specific activity of 25-kDa ThTPase (see below). ThTP is always observed in brain and skeletal muscle. It has been proposed that, in skeletal muscle, ThTP is synthesized by AK1 , , while in brain it is synthesized in mitochondria by a chemiosmotic mechanism . In the latter case, ThTP can be released from the mitochondria and may be subsequently hydrolyzed by the cytosolic ThTPase.
As for other thiamine derivatives, inter-patient variability was very high for ThTP. In some patients, ThTP was undetectable while in other patients the same tissue contained a relatively high proportion of ThTP. An inverse relationship was observed for thiamine. Therefore, we defined a new parameter, the TPR, reflecting the degree of phosphorylation of thiamine derivatives. This parameter, at least in cardiovascular tissues, seems to be a characteristic of a given patient, at least at a precise moment of his or her life. This parameter tended to be lower in patients suffering from cardiac insufficiency. It is well known that severe thiamine deficiency is often accompanied by acute congestive heart failure. A recent study showed that thiamine deficiency-induced heart failure in the rat involves oxidative stress induced apoptosis . Furthermore, thiamine deficiency impairs contractile function in cardiomyocytes . Though the heart failure is generally attributed to decreased ThDP-dependent enzyme activities, our results suggest that ThTP may also be involved in this phenomenon. Indeed, during thiamine deficiency, both ThDP and ThTP are reduced and our recent results show that ThTP synthesis is probably mostly mitochondrial in the heart .
ThTP was rarely observed in gynecological specimen, but was nearly always present in fetal tissue-derived samples. This might be the consequence of a lower 25-kDa ThTPase activity in the latter.
ThTP levels are probably regulated by cytosolic 25-kDa ThTPase, an enzyme mainly expressed in differentiated cells
ThTP may accumulate in tissues devoid of this enzyme activity, such as Electrophorus electricus electric organ , pig skeletal muscle  or chicken skeletal muscle . Pig and quail brains also have a higher ThTP content than rodent brain (Table 7), which has a high ThTPase activity. The inverse relationship between ThTPase activity and ThTP content also holds for human tissues (Tables 4 and 5): 25-kDa ThTPase activity is lower and ThTP content is higher in fetal tissue and trophoblast than in uterus, for instance. ThTPase activity appeared to correlate with the protein expression as determined by Western blotting (Fig. 4). Strangely, in human cell lines, ThTPase activity, as well as the ThTPase protein, were undetectable. These results suggest that ThTPase expression is linked to the degree of differentiation of the cells. Highly differentiated and quiescent cells such as neurons  and, in general, adult tissue in rodents  and humans (this study) contain a higher ThTPase activity than non-differentiated fast-growing cells. Though the ThTPase mRNA expression does not correlate with enzyme activities , using a semi-quantitative profiling of ThTPase mRNA on a human multiple tissue expression array, we found a low expression in fetal tissue and the highest expression in uterus, testis and prostate, with intermediate expression in liver, skeletal muscle or brain . An increase in 25-kDa ThTPase activity after birth was also observed in rat brain and liver .
AThTP is a minor compound mainly present poorly differentiated fast-growing cells
We show for the first time the existence of AThTP and, very sporadically, of AThDP in human tissues. Interestingly, AThTP is found mainly in fetal or fetus-derived tissues and also in cultured cells but rarely in well-differentiated tissues. Moreover, its presence is at least statistically linked to the presence of detectable amounts of ThTP. It is indeed rarely seen in the absence of ThTP, both biopsies and in cultured cells. This is in contrast to E. coli cells in which both compounds to not accumulate together. In E. coli, AThTP accumulates in response to carbon starvation or collapse of the transmembrane proton gradient , , but we have no clue as to the regulation of AThTP levels in animal tissues.
In summary, the present data substantiate the view that thiamine status is particularly low in humans compared to other mammalian species (especially rodents). This justifies the proposal that thiamine supplementation should be envisaged in many pathological states, especially in elderly patients. An interesting new finding is that ThTP is found in many human tissues at a concentration higher than in other mammalian species, and this is likely due to a lower expression of the specific 25-kDa ThTPase. The possible physiological roles of ThTP and ThTPase are a subject of active investigation in our laboratory. We show for the first time the occurrence of adenylated thiamine derivatives in human tissues. AThTP was found sporadically in many samples, but very consistently in fetal and fetal-derived tissues and cultured cells. It might thus play a role in rapidly dividing cells.
Materials and Methods
All solutions were prepared using milli-Q water (Millipore S.A./N.V., Brussels, Belgium) and all the solvents used for chromatography were of HPLC grade (Biosolve, Valkenswaard, The Netherlands). Thiamine, ThMP and ThDP were from Sigma-Aldrich NV/SA (Bornem, Belgium). ThTP and AThTP, which are not commercially available, were synthesized as described , . All other chemicals were from Merck (Darmstadt, Germany) or from Sigma-Aldrich and of the highest purity available.
The experimental procedures were in accordance with the Declaration of Helsinki and were approved by the ethical committees of the Hospital of the University of Liège and the Citadelle Hospital (Liège). All tissue donors provided written informed consent.
All animal experiments were made in accordance with the directives of the committee for animal care and use of the University of Liège and in accordance with the European Communities Council Directive of November 24, 1986 (86/609/EEC). The protocols were approved by the Committee on the Ethics of Animal Experiments of the University of Liège (# 823 for rats and # 727 for quails). The animals were killed by decapitation and all efforts were made to minimize suffering.
Mouse neuroblastoma cells (neuro-2a, ATCC n° CCL-131), mouse myoblasts (C2C12, ATCC n° CRL-1772) and mouse fibroblasts (3T3, ATCC n° CRL-1658) were grown at 37°C in a humidified atmosphere of 95% air, 5% CO2, in 10 cm Petri dishes (Greiner Bio-One N.V./S.A., Wemmel, Belgium) in 10 mL of Dulbecco's modified Eagle's medium (Invitrogen Life Technologies, Carlsbad, CA, USA) supplemented with fetal bovine serum (10%). Cells were subcultured to a fresh culture dish when growth reached 70–90% confluence, i.e. every 2–3 days. Human neuroblastoma cells (SK-N-BE, ATCC n° RL-2271), human glioblastoma (LN-18, ATCC n° CRL-2610) and rat phaeochromocytoma cells (PC-12, ATCC n° CRL-1721) were grown at 37°C in a humidified atmosphere of 95% air, 5% CO2 in 10 cm Petri dishes in 8 mL of DMEM supplemented with 10% heat-inactivated horse serum (Invitrogen) and 5% fetal bovine serum. The medium was changed every 2 days of culture and cells were subcultured to a fresh culture dish when growth reached 70–90% of confluence.
Preparation of tissue extracts or cultured cells for the determination of thiamine derivatives
Human biopsies were frozen either in liquid nitrogen or on dry ice as rapidly as possible after removal and stored at −80°C until use. Blood samples were analyzed immediately, without freezing. Male Wistar rats (200 g) and adult quails (14 weeks old) were from the institutional animal facility and killed by decapitation. Pig brains were from the local slaughterhouse and immediately frozen on dry ice.
Frozen tissues (approximately 10–50 mg) were homogenized in 10–50 volumes (0.5–1 mL) of trichloroacetic acid (TCA, 12%) in a glass-glass Potter-Elvehjem homogenizer. In some cases, liver samples were homogenized in a medium containing D-mannitol 225 mM), sucrose (75 mM), Hepes (7.5 mM), EDTA (0.5 mM), BSA fatty acid free (0.1%, w/v), pH adjusted to 7.3 with KOH. The homogenate was centrifuged (100 000× g, 60 min) to obtain the cytosolic and the particulate fractions.
Whole semen was precipitated with 2 volumes of TCA (18%). In some cases, fresh semen was centrifuged (500× g, 10 min) to obtain the seminal fluid that was also precipitated with 2 volumes of TCA (18%). We used computer-assisted semen analysis (AutoSperm, MedCalc Software bvba, Mariakerke, Belgium) for the determination of sperm concentration and motility . An aliquot from a homogeneous, mixed semen sample was placed in counting chamber at room temperature and recorded by a video camera. Sperm morphology was assessed using the Krüger strict criteria .
Venous blood samples (2×5 mL) were taken from healthy volunteers using EDTA blood collection tubes. The blood was treated with 2 volumes of TCA (18%). For the analysis of human plasma, the blood was centrifuged (1100× g, 15 min, 4°C) and the supernatant treated with 0.2 volumes of 72% TCA.
Cultured cells were detached with trypsin, centrifuged (350× g, 3 min) and the pellet suspended in 500 µL of 72% TCA.
All the samples were centrifuged (5000× g, 5 min), and the TCA was removed by diethyl ether extraction (3×1.5 mL). They were stored frozen at −20°C until determination of thiamine derivatives by HPLC.
Determination of thiamine derivatives by HPLC
Thiamine compounds were determined by HPLC as previously described after transformation into fluorescent thiochrome derivatives , . Prior to analysis, an 80 µl aliquot was oxidized with 50 µl of 4.3 mM potassium ferricyanide in 15% NaOH and a 20-µl sample volume was injected into the chromatographic system (System 522, Kontron Instruments, Milan, Italy). The separation was performed at a flow rate of 0.5 ml/min on a PRP-1 column (Ø 4.1×150 mm, Hamilton Co, Reno, NV, USA) in 50 mM NaH2PO4 containing 25 mM tetra-n-butylammonium hydrogen sulfate and 4% tetrahydrofuran and adjusted to pH 9.5 with NaOH. Thiochrome derivatives were quantified using a fluorometric spectrometer (LS-4, Perkin-Elmer, Shelton, CT, USA, or SFM 25, Kontron Instruments). Proteins were determined in the pellets by the method of Peterson  after solubilization in an initial volume of 1 N NaOH.
In some cases, the presence of authentic ThTP was demonstrated by hydrolysis with the specific recombinant mouse 25-kDa ThTPase . The extract (80 µL) was mixed with 15 µL of Bis-Tris-Propane buffer (0.5 M, pH 8.9), 5 µL MgCl2 (0.1 M) and 2 µL of the purified recombinant mThTPase (diluted 100×) at 37°C. After incubation (15 min, 37°C), the mixture was put on ice and analyzed by HPLC. In control experiments, the enzyme was replaced by 2 µL H2O.
Preparation of tissue extracts for the determination of 25-kDa ThTPase activity and Western blots
Human tissues were homogenized in a glass-glass Potter-Elvehjem homogenizer with 5 volumes of dilute Tris buffer (5 mM Tris-HCl, 0.5 mM EDTA, pH 8.2) supplemented with protease inhibitors (complete mini EDTA-free Protease Inhibitor Cocktail Tablets, Roche Applied Science, Vilvoorde, Belgium). Homogenates were centrifuged at 4°C for 30 min at 20 000× g. The supernatant was used for the determination of ThTPase and protein concentrations as well as for Western blots.
RT-PCR detection of 25-kDa ThTPase mRNA in human and mouse cells
Total RNA was extracted from mouse and human cells using RNA InstaPure System (Eurogentec, Belgium) according to the manufacturer's instruction. It was then transcribed in cDNA in 20 µl solution containing 5 µg of total RNA (Promega Benelux b.v., Leiden, The Netherlands).
ThTPase-specific fragments were amplified using specific forward primers (GCT GCA GGA AGT AGC TAG TT and GCA GGA GGT GGC TAG TTT T) and reverse primers (AGC TGT TCA CTT CTA GCA GG and AGA ACT GGC TGC TTC TAG CA) for human and mouse mRNA sequences. The sequence amplified by these primers ranged from nucleotides 342 to 643 and 345 to 645 on human and mouse coding sequence respectively. The PCR reactions using these primers were composed of 1 µl cDNA, 5 µl of each primer (100 µM) and 0.5 µl (2.5 U) Go Taq DNA polymerase in a final volume of 50 µl. PCR cycling parameters consisted of 5 min at 95°C and 35 cycles of 30 s at 94°C, 30 s at 55°C, and 30 s at 72°C, with a final step of 5 min at 72°C (T3000 Thermocycler, Biometra GmbH, Goettingen, Germany). Amplified products were visualized with ethidium bromide staining after electrophoresis on 1% agarose gel. The sequence of PCR products was confirmed by nucleic acid sequencing (GIGA, University of Liège).
ThTPase activity measurements
Supernatant fractions from tissue or cell homogenates were used for the determination of ThTPase activity . The rate of ThTP hydrolysis was measured in 50 mM Tris-HCl buffer, pH 8.5 in the presence of 10 mM MgCl2 and 10 µM ThTP in a final volume of 100 µl. The reaction was stopped by addition of 0.5 mL of 12% TCA. The samples were centrifuged (5000× g, 5 min) and extracted with diethyl ether. The disappearance of ThTP and the production of ThDP were determined by HPLC .
Soluble cytosolic fractions were separated by SDS-PAGE electrophoresis using 0.75 mm thick 12% acrylamide gels. 40 µg of proteins were loaded per well and electrophoresis was carried out at room temperature for 1 h using 20 mA current per gel. 0.5 µg of purified human recombinant 25-kDa ThTPase was used as reference . Proteins were transferred to Hybond-P PVDF Membrane (GE Healthcare Europe GmbH, 201831 Diegem, Belgium) using wet transfer at 4°C for 2 h at 50 V. Membranes were treated for 1 h with TBS-T (50 mM Tris-HCl, pH 7.6, 150 mM NaCl, 0.2% Tween 20, pH 7.4) containing 5% dry milk and then incubated overnight at 4°C with mouse anti-ThTPA poly- and monoclonal (clone 3F6) antibodies (Abnova GmbH, 69126 Heidelberg, Germany) diluted 1∶3000 in TBS-T with 5% dry milk. After incubation, the membranes were washed 3 times for 5 min in TBS-T and incubated at room temperature for 1 h with peroxidase-conjugated AffiniPure F(ab')2 fragment rat anti-mouse secondary antibodies diluted 1∶3000 in TBS-T with 5% dry milk. Then the membranes were washed three times in TBS-T and the protein bands were revealed by incubation for 60 s at room temperature using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Fisher Scientific, Tournai, Belgium).
ANOVA and linear regression analysis were made using Prism 4 or InStat 3 for Macintosh (GraphPad Software). In some cases post hoc comparisons were made using Tukey's test. Coefficients of variation (Cv) were calculated as the ratios of the standard deviation (SD) to the mean, Cv = SD/Mean. Contingency tables were analyzed using Fisher's exact test.
Western blots of human tissue and cultured cell lines using a commercial mouse monoclonal antibody.
(0.28 MB DOC)
The authors wish to thank M. Merciny (Department of Cardiovascular Surgery), C. Blockx, B. Balteau and P. Ruggeri (Department of Gynecology-Obstetrics) for help with sample collection; C. Gerday (Department of Gynecology-Obstetrics) for help with the ethical committee and I. Margineanu for reading the manuscript. Quails were from Prof. J. Balthazart (Behavioral Neuroendocrinology, GIGA-Neurosciences, University of Liege). Nucleic acid sequencing was performed by V. Dhennin, Genotranscriptomics Platform, GIGA, University of Liège, http://www.giga.ulg.ac.be/.
Conceived and designed the experiments: BL PW TG LB. Performed the experiments: MG JC LB. Analyzed the data: MG JC MR CJ BL PW TG LB. Contributed reagents/materials/analysis tools: MR OD MN CJ DM FC BL LB. Wrote the paper: PW LB.
- 1. McCandless DW (2009) Thiamine deficiency and associated clinical disorders;. In: Lydic R, Baghdoyan HA, editors. Springer. DW McCandless2009Thiamine deficiency and associated clinical disorders;R. LydicHA BaghdoyanSpringer
- 2. Kopelman MD, Thomson AD, Guerrini I, Marshall EJ (2009) The Korsakoff syndrome: clinical aspects, psychology and treatment. Alcohol Alcohol 44: 148–154.MD KopelmanAD ThomsonI. GuerriniEJ Marshall2009The Korsakoff syndrome: clinical aspects, psychology and treatment.Alcohol Alcohol44148154
- 3. Harper CG, Giles M, Finlay-Jones R (1986) Clinical signs in the Wernicke-Korsakoff complex: a retrospective analysis of 131 cases diagnosed at necropsy. J Neurol Neurosurg Psychiatry 49: 341–345.CG HarperM. GilesR. Finlay-Jones1986Clinical signs in the Wernicke-Korsakoff complex: a retrospective analysis of 131 cases diagnosed at necropsy.J Neurol Neurosurg Psychiatry49341345
- 4. Smidt LJ, Cremin FM, Grivetti LE, Clifford AJ (1991) Influence of thiamin supplementation on the health and general well-being of an elderly Irish population with marginal thiamin deficiency. J Gerontol 46: M16–22.LJ SmidtFM CreminLE GrivettiAJ Clifford1991Influence of thiamin supplementation on the health and general well-being of an elderly Irish population with marginal thiamin deficiency.J Gerontol46M1622
- 5. Chen MF, Chen LT, Gold M, Boyce HW Jr (1996) Plasma and erythrocyte thiamin concentrations in geriatric outpatients. J Am Coll Nutr 15: 231–236.MF ChenLT ChenM. GoldHW Boyce Jr1996Plasma and erythrocyte thiamin concentrations in geriatric outpatients.J Am Coll Nutr15231236
- 6. Wilkinson TJ, Hanger HC, Elmslie J, George PM, Sainsbury R (1997) The response to treatment of subclinical thiamine deficiency in the elderly. Am J Clin Nutr 66: 925–928.TJ WilkinsonHC HangerJ. ElmsliePM GeorgeR. Sainsbury1997The response to treatment of subclinical thiamine deficiency in the elderly.Am J Clin Nutr66925928
- 7. Vognar L, Stoukides J (2009) The role of low plasma thiamin levels in cognitively impaired elderly patients presenting with acute behavioral disturbances. J Am Geriatr Soc 57: 2166–2168.L. VognarJ. Stoukides2009The role of low plasma thiamin levels in cognitively impaired elderly patients presenting with acute behavioral disturbances.J Am Geriatr Soc5721662168
- 8. Butterworth RF, Gaudreau C, Vincelette J, Bourgault AM, Lamothe F, et al. (1991) Thiamine deficiency and Wernicke's encephalopathy in AIDS. Metab Brain Dis 6: 207–212.RF ButterworthC. GaudreauJ. VinceletteAM BourgaultF. Lamothe1991Thiamine deficiency and Wernicke's encephalopathy in AIDS.Metab Brain Dis6207212
- 9. Müri RM, Von Overbeck J, Furrer J, Ballmer PE (1999) Thiamin deficiency in HIV-positive patients: evaluation by erythrocyte transketolase activity and thiamin pyrophosphate effect. Clin Nutr 18: 375–378.RM MüriJ. Von OverbeckJ. FurrerPE Ballmer1999Thiamin deficiency in HIV-positive patients: evaluation by erythrocyte transketolase activity and thiamin pyrophosphate effect.Clin Nutr18375378
- 10. Harper C (2006) Thiamine (vitamin B1) deficiency and associated brain damage is still common throughout the world and prevention is simple and safe! Eur J Neurol 13: 1078–1082.C. Harper2006Thiamine (vitamin B1) deficiency and associated brain damage is still common throughout the world and prevention is simple and safe!Eur J Neurol1310781082
- 11. van Zaanen HC, van der Lelie J (1992) Thiamine deficiency in hematologic malignant tumors. Cancer 69: 1710–1713.HC van ZaanenJ. van der Lelie1992Thiamine deficiency in hematologic malignant tumors.Cancer6917101713
- 12. Butterworth RF (2009) Thiamine deficiency-related brain dysfunction in chronic liver failure. Metab Brain Dis 24: 189–196.RF Butterworth2009Thiamine deficiency-related brain dysfunction in chronic liver failure.Metab Brain Dis24189196
- 13. Shimomura T, Mori E, Hirono N, Imamura T, Yamashita H (1998) Development of Wernicke-Korsakoff syndrome after long intervals following gastrectomy. Arch Neurol 55: 1242–1245.T. ShimomuraE. MoriN. HironoT. ImamuraH. Yamashita1998Development of Wernicke-Korsakoff syndrome after long intervals following gastrectomy.Arch Neurol5512421245
- 14. Fattal-Valevski A, Kesler A, Sela BA, Nitzan-Kaluski D, Rotstein M, et al. (2005) Outbreak of life-threatening thiamine deficiency in infants in Israel caused by a defective soy-based formula. Pediatrics 115: e233–e238.A. Fattal-ValevskiA. KeslerBA SelaD. Nitzan-KaluskiM. Rotstein2005Outbreak of life-threatening thiamine deficiency in infants in Israel caused by a defective soy-based formula.Pediatrics115e233e238
- 15. Scileppi KP, Blass JP, Baker HG (1984) Circulating vitamins in Alzheimer's dementia as compared with other dementias. J Am Geriatr Soc 32: 709–711.KP ScileppiJP BlassHG Baker1984Circulating vitamins in Alzheimer's dementia as compared with other dementias.J Am Geriatr Soc32709711
- 16. Molina JA, Jimenez-Jimenez FJ, Hernanz A, Fernandez-Vivancos E, Medina S, et al. (2002) Cerebrospinal fluid levels of thiamine in patients with Alzheimer's disease. J Neural Transm 109: 1035–1044.JA MolinaFJ Jimenez-JimenezA. HernanzE. Fernandez-VivancosS. Medina2002Cerebrospinal fluid levels of thiamine in patients with Alzheimer's disease.J Neural Transm10910351044
- 17. Gold M, Chen MF, Johnson K (1995) Plasma and red blood cell thiamine deficiency in patients with dementia of the Alzheimer's type. Arch Neurol 52: 1081–1086.M. GoldMF ChenK. Johnson1995Plasma and red blood cell thiamine deficiency in patients with dementia of the Alzheimer's type.Arch Neurol5210811086
- 18. Mastrogiacomo F, Bettendorff L, Grisar T, Kish SJ (1996) Brain thiamine, its phosphate esters, and its metabolizing enzymes in Alzheimer's disease. Ann Neurol 39: 585–591.F. MastrogiacomoL. BettendorffT. GrisarSJ Kish1996Brain thiamine, its phosphate esters, and its metabolizing enzymes in Alzheimer's disease.Ann Neurol39585591
- 19. Heroux M, Raghavendra Rao VL, Lavoie J, Richardson JS, Butterworth RF (1996) Alterations of thiamine phosphorylation and of thiamine-dependent enzymes in Alzheimer's disease. Metab Brain Dis 11: 81–88.M. HerouxVL Raghavendra RaoJ. LavoieJS RichardsonRF Butterworth1996Alterations of thiamine phosphorylation and of thiamine-dependent enzymes in Alzheimer's disease.Metab Brain Dis118188
- 20. Bettendorff L, Mastrogiacomo F, Wins P, Kish SJ, Grisar T, et al. (1997) Low thiamine diphosphate levels in brains of patients with frontal lobe degeneration of the non-Alzheimer's type. J Neurochem 69: 2005–2010.L. BettendorffF. MastrogiacomoP. WinsSJ KishT. Grisar1997Low thiamine diphosphate levels in brains of patients with frontal lobe degeneration of the non-Alzheimer's type.J Neurochem6920052010
- 21. Bettendorff L, Wins P (2009) Thiamin diphosphate in biological chemistry: New aspects of thiamin metabolism, especially triphosphate derivatives acting other than as cofactors. FEBS J 276: 2917–2925.L. BettendorffP. Wins2009Thiamin diphosphate in biological chemistry: New aspects of thiamin metabolism, especially triphosphate derivatives acting other than as cofactors.FEBS J27629172925
- 22. Makarchikov AF, Lakaye B, Gulyai IE, Czerniecki J, Coumans B, et al. (2003) Thiamine triphosphate and thiamine triphosphatase activities: from bacteria to mammals. Cell Mol Life Sci 60: 1477–1488.AF MakarchikovB. LakayeIE GulyaiJ. CzernieckiB. Coumans2003Thiamine triphosphate and thiamine triphosphatase activities: from bacteria to mammals.Cell Mol Life Sci6014771488
- 23. Nghiêm HO, Bettendorff L, Changeux JP (2000) Specific phosphorylation of Torpedo 43K rapsyn by endogenous kinase(s) with thiamine triphosphate as the phosphate donor. FASEB J 14: 543–554.HO NghiêmL. BettendorffJP Changeux2000Specific phosphorylation of Torpedo 43K rapsyn by endogenous kinase(s) with thiamine triphosphate as the phosphate donor.FASEB J14543554
- 24. Bettendorff L, Kolb HA, Schoffeniels E (1993) Thiamine triphosphate activates an anion channel of large unit conductance in neuroblastoma cells. J Membr Biol 136: 281–288.L. BettendorffHA KolbE. Schoffeniels1993Thiamine triphosphate activates an anion channel of large unit conductance in neuroblastoma cells.J Membr Biol136281288
- 25. Shikata H, Koyama S, Egi Y, Yamada K, Kawasaki T (1989) Cytosolic adenylate kinase catalyzes the synthesis of thiamin triphosphate from thiamin diphosphate. Biochem Int 18: 933–941.H. ShikataS. KoyamaY. EgiK. YamadaT. Kawasaki1989Cytosolic adenylate kinase catalyzes the synthesis of thiamin triphosphate from thiamin diphosphate.Biochem Int18933941
- 26. Miyoshi K, Egi Y, Shioda T, Kawasaki T (1990) Evidence for in vivo synthesis of thiamin triphosphate by cytosolic adenylate kinase in chicken skeletal muscle. J Biochem (Tokyo) 108: 267–270.K. MiyoshiY. EgiT. ShiodaT. Kawasaki1990Evidence for in vivo synthesis of thiamin triphosphate by cytosolic adenylate kinase in chicken skeletal muscle.J Biochem (Tokyo)108267270
- 27. Gigliobianco T, Lakaye B, Makarchikov AF, Wins P, Bettendorff L (2008) Adenylate kinase-independent thiamine triphosphate accumulation under severe energy stress in Escherichia coli. BMC Microbiol 8: 16.T. GigliobiancoB. LakayeAF MakarchikovP. WinsL. Bettendorff2008Adenylate kinase-independent thiamine triphosphate accumulation under severe energy stress in Escherichia coli.BMC Microbiol816
- 28. Gangolf M, Wins P, Thiry M, El Moualij B, Bettendorff L (2010) Thiamine triphosphate synthesis in rat brain occurs in mitochondria and is coupled to the respiratory chain. J Biol Chem 285: 583–594.M. GangolfP. WinsM. ThiryB. El MoualijL. Bettendorff2010Thiamine triphosphate synthesis in rat brain occurs in mitochondria and is coupled to the respiratory chain.J Biol Chem285583594
- 29. Barchi RL, Braun PE (1972) A membrane-associated thiamine triphosphatase from rat brain. Properties of the enzyme. J Biol Chem 247: 7668–7673.RL BarchiPE Braun1972A membrane-associated thiamine triphosphatase from rat brain. Properties of the enzyme.J Biol Chem24776687673
- 30. Bettendorff L, Michel-Cahay C, Grandfils C, De Rycker C, Schoffeniels E (1987) Thiamine triphosphate and membrane-associated thiamine phosphatases in the electric organ of Electrophorus electricus. J Neurochem 49: 495–502.L. BettendorffC. Michel-CahayC. GrandfilsC. De RyckerE. Schoffeniels1987Thiamine triphosphate and membrane-associated thiamine phosphatases in the electric organ of Electrophorus electricus.J Neurochem49495502
- 31. Bettendorff L, Wins P, Schoffeniels E (1988) Thiamine triphosphatase from Electrophorus electric organ is anion-dependent and irreversibly inhibited by 4,4′-diisothiocyanostilbene-2,2′disulfonic acid. Biochem Biophys Res Commun 154: 942–947.L. BettendorffP. WinsE. Schoffeniels1988Thiamine triphosphatase from Electrophorus electric organ is anion-dependent and irreversibly inhibited by 4,4′-diisothiocyanostilbene-2,2′disulfonic acid.Biochem Biophys Res Commun154942947
- 32. Makarchikov AF, Chernikevich IP (1992) Purification and characterization of thiamine triphosphatase from bovine brain. Biochim Biophys Acta 1117: 326–332.AF MakarchikovIP Chernikevich1992Purification and characterization of thiamine triphosphatase from bovine brain.Biochim Biophys Acta1117326332
- 33. Iyer LM, Aravind L (2002) The catalytic domains of thiamine triphosphatase and CyaB-like adenylyl cyclase define a novel superfamily of domains that bind organic phosphates. BMC Genomics 3: 33.LM IyerL. Aravind2002The catalytic domains of thiamine triphosphatase and CyaB-like adenylyl cyclase define a novel superfamily of domains that bind organic phosphates.BMC Genomics333
- 34. Gong C, Smith P, Shuman S (2006) Structure-function analysis of Plasmodium RNA triphosphatase and description of a triphosphate tunnel metalloenzyme superfamily that includes Cet1-like RNA triphosphatases and CYTH proteins. RNA 12: 1468–1474.C. GongP. SmithS. Shuman2006Structure-function analysis of Plasmodium RNA triphosphatase and description of a triphosphate tunnel metalloenzyme superfamily that includes Cet1-like RNA triphosphatases and CYTH proteins.RNA1214681474
- 35. Song J, Bettendorff L, Tonelli M, Markley JL (2008) Structural basis for the catalytic mechanism of mammalian 25-kDa thiamine triphosphatase. J Biol Chem 283: 10939–10948.J. SongL. BettendorffM. TonelliJL Markley2008Structural basis for the catalytic mechanism of mammalian 25-kDa thiamine triphosphatase.J Biol Chem2831093910948
- 36. Lakaye B, Makarchikov AF, Antunes AF, Zorzi W, Coumans B, et al. (2002) Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues. J Biol Chem 277: 13771–13777.B. LakayeAF MakarchikovAF AntunesW. ZorziB. Coumans2002Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues.J Biol Chem2771377113777
- 37. Lakaye B, Verlaet M, Dubail J, Czerniecki J, Bontems S, et al. (2004) Expression of 25 kDa thiamine triphosphatase in rodent tissues using quantitative PCR and characterization of its mRNA. Int J Biochem Cell Biol 36: 2032–2041.B. LakayeM. VerlaetJ. DubailJ. CzernieckiS. Bontems2004Expression of 25 kDa thiamine triphosphatase in rodent tissues using quantitative PCR and characterization of its mRNA.Int J Biochem Cell Biol3620322041
- 38. Czerniecki J, Chanas G, Verlaet M, Bettendorff L, Makarchikov AF, et al. (2004) Neuronal localization of the 25-kDa specific thiamine triphosphatase in rodent brain. Neuroscience 125: 833–840.J. CzernieckiG. ChanasM. VerlaetL. BettendorffAF Makarchikov2004Neuronal localization of the 25-kDa specific thiamine triphosphatase in rodent brain.Neuroscience125833840
- 39. Bettendorff L, Wirtzfeld B, Makarchikov AF, Mazzucchelli G, Frédérich M, et al. (2007) Discovery of a natural thiamine adenine nucleotide. Nat Chem Biol 3: 211–212.L. BettendorffB. WirtzfeldAF MakarchikovG. MazzucchelliM. Frédérich2007Discovery of a natural thiamine adenine nucleotide.Nat Chem Biol3211212
- 40. Gigliobianco T, Lakaye B, Wins P, El Moualij B, Zorzi W, et al. (2010) Adenosine thiamine triphosphate accumulates in Escherichia coli cells in response to specific conditions of metabolic stress. BMC Microbiol 10: 148.T. GigliobiancoB. LakayeP. WinsB. El MoualijW. Zorzi2010Adenosine thiamine triphosphate accumulates in Escherichia coli cells in response to specific conditions of metabolic stress.BMC Microbiol10148
- 41. Makarchikov AF, Brans A, Bettendorff L (2007) Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphate BMC Biochem 8: 17.AF MakarchikovA. BransL. Bettendorff2007Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphate BMC Biochem817
- 42. Frédérich M, Delvaux D, Gigliobianco T, Gangolf M, Dive G, et al. (2009) Thiaminylated adenine nucleotides. Chemical synthesis, structural characterization and natural occurrence FEBS J 276: 3256–3268.M. FrédérichD. DelvauxT. GigliobiancoM. GangolfG. Dive2009Thiaminylated adenine nucleotides.Chemical synthesis, structural characterization and natural occurrence FEBS J27632563268
- 43. Cooper JR, Itokawa Y, Pincus JH (1969) Thiamine triphosphate deficiency in subacute necrotizing encephalomyelopathy. Science 164: 74–75.JR CooperY. ItokawaJH Pincus1969Thiamine triphosphate deficiency in subacute necrotizing encephalomyelopathy.Science1647475
- 44. Pincus JH, Solitare GB, Cooper JR (1976) Thiamine triphosphate levels and histopathology. Correlation in Leigh disease. Arch Neurol 33: 759–763.JH PincusGB SolitareJR Cooper1976Thiamine triphosphate levels and histopathology. Correlation in Leigh disease.Arch Neurol33759763
- 45. Schrijver J, Dias T, Hommes FA (1978) Studies on ATP: thiamine diphosphate phosphotransferase activity in rat brain. Neurochem Res 3: 699–709.J. SchrijverT. DiasFA Hommes1978Studies on ATP: thiamine diphosphate phosphotransferase activity in rat brain.Neurochem Res3699709
- 46. Bettendorff L, Mastrogiacomo F, Kish SJ, Grisar T (1996) Thiamine, thiamine phosphates, and their metabolizing enzymes in human brain. J Neurochem 66: 250–258.L. BettendorffF. MastrogiacomoSJ KishT. Grisar1996Thiamine, thiamine phosphates, and their metabolizing enzymes in human brain.J Neurochem66250258
- 47. Siesjö BK (1978) Brain Energy Metabolism. New York: J. Wiley and Sons. BK Siesjö1978Brain Energy Metabolism.New YorkJ. Wiley and Sons
- 48. Bettendorff L, Schoffeniels E, Naquet R, Silva-Barrat C, Riche D, et al. (1989) Phosphorylated thiamine derivatives and cortical activity in the baboon Papio papio: effect of intermittent light stimulation. J Neurochem 53: 80–87.L. BettendorffE. SchoffenielsR. NaquetC. Silva-BarratD. Riche1989Phosphorylated thiamine derivatives and cortical activity in the baboon Papio papio: effect of intermittent light stimulation.J Neurochem538087
- 49. Bettendorff L (1994) The compartmentation of phosphorylated thiamine derivatives in cultured neuroblastoma cells. Biochim Biophys Acta 1222: 7–14.L. Bettendorff1994The compartmentation of phosphorylated thiamine derivatives in cultured neuroblastoma cells.Biochim Biophys Acta1222714
- 50. Laforenza U, Patrini C, Alvisi C, Faelli A, Licandro A, et al. (1997) Thiamine uptake in human intestinal biopsy specimens, including observations from a patient with acute thiamine deficiency. Am J Clin Nutr 66: 320–326.U. LaforenzaC. PatriniC. AlvisiA. FaelliA. Licandro1997Thiamine uptake in human intestinal biopsy specimens, including observations from a patient with acute thiamine deficiency.Am J Clin Nutr66320326
- 51. Reidling JC, Subramanian VS, Dudeja PK, Said HM (2002) Expression and promoter analysis of SLC19A2 in the human intestine. Biochim Biophys Acta 1561: 180–187.JC ReidlingVS SubramanianPK DudejaHM Said2002Expression and promoter analysis of SLC19A2 in the human intestine.Biochim Biophys Acta1561180187
- 52. Casirola D, Patrini C, Ferrari G, Rindi G (1990) Thiamin transport by human erythrocytes and ghosts. J Membr Biol 118: 11–18.D. CasirolaC. PatriniG. FerrariG. Rindi1990Thiamin transport by human erythrocytes and ghosts.J Membr Biol1181118
- 53. Kimura M, Itokawa Y (1985) Determination of thiamine and its phosphate esters in human and rat blood by high-performance liquid chromatography with post-column derivatization. J Chromatogr 332: 181–188.M. KimuraY. Itokawa1985Determination of thiamine and its phosphate esters in human and rat blood by high-performance liquid chromatography with post-column derivatization.J Chromatogr332181188
- 54. Egi Y, Koyama S, Shioda T, Yamada K, Kawasaki T (1992) Identification, purification and reconstitution of thiamin metabolizing enzymes in human red blood cells. Biochim Biophys Acta 1160: 171–178.Y. EgiS. KoyamaT. ShiodaK. YamadaT. Kawasaki1992Identification, purification and reconstitution of thiamin metabolizing enzymes in human red blood cells.Biochim Biophys Acta1160171178
- 55. Kawasaki T (1992) Vitamin B1: Thiamine. In: De Leenheer AP, Lambert WE, Nelis HJ, editors. Modern Chromatographic Analysis of Vitamins. 2nd ed. New York: Marcel Dekker, Inc. pp. 319–354.T. Kawasaki1992Vitamin B1: Thiamine.AP De LeenheerWE LambertHJ NelisModern Chromatographic Analysis of Vitamins. 2nd edNew YorkMarcel Dekker, Inc319354
- 56. Fayol V (1997) High-performance liquid chromatography determination of total thiamin in biological and food products. Methods Enzymol 279: 57–66.V. Fayol1997High-performance liquid chromatography determination of total thiamin in biological and food products.Methods Enzymol2795766
- 57. Floridi A, Pupita M, Palmerini CA, Fini C, Alberti Fidanza A (1984) Thiamine pyrophosphate determination in whole blood and erythrocytes by high performance liquid chromatography. Int J Vitam Nutr Res 54: 165–171.A. FloridiM. PupitaCA PalmeriniC. FiniA. Alberti Fidanza1984Thiamine pyrophosphate determination in whole blood and erythrocytes by high performance liquid chromatography.Int J Vitam Nutr Res54165171
- 58. Brunnekreeft JW, Eidhof H, Gerrits J (1989) Optimized determination of thiochrome derivatives of thiamine and thiamine phosphates in whole blood by reversed-phase liquid chromatography with precolumn derivatization. J Chromatogr 491: 89–96.JW BrunnekreeftH. EidhofJ. Gerrits1989Optimized determination of thiochrome derivatives of thiamine and thiamine phosphates in whole blood by reversed-phase liquid chromatography with precolumn derivatization.J Chromatogr4918996
- 59. Tallaksen CM, Bohmer T, Bell H, Karlsen J (1991) Concomitant determination of thiamin and its phosphate esters in human blood and serum by high-performance liquid chromatography. J Chromatogr 564: 127–136.CM TallaksenT. BohmerH. BellJ. Karlsen1991Concomitant determination of thiamin and its phosphate esters in human blood and serum by high-performance liquid chromatography.J Chromatogr564127136
- 60. Lu J, Frank EL (2008) Rapid HPLC Measurement of Thiamine and its Phosphate Esters in Whole Blood. Clin Chem 54: 901–906.J. LuEL Frank2008Rapid HPLC Measurement of Thiamine and its Phosphate Esters in Whole Blood.Clin Chem54901906
- 61. Bettendorff L, Grandfils C, De Rycker C, Schoffeniels E (1986) Determination of thiamine and its phosphate esters in human blood serum at femtomole levels. J Chromatogr 382: 297–302.L. BettendorffC. GrandfilsC. De RyckerE. Schoffeniels1986Determination of thiamine and its phosphate esters in human blood serum at femtomole levels.J Chromatogr382297302
- 62. Weber W, Kewitz H (1985) Determination of thiamine in human plasma and its pharmacokinetics. Eur J Clin Pharmacol 28: 213–219.W. WeberH. Kewitz1985Determination of thiamine in human plasma and its pharmacokinetics.Eur J Clin Pharmacol28213219
- 63. Kimura M, Fujita T, Itokawa Y (1982) Liquid-chromatographic determination of the total thiamin content of blood. Clin Chem 28: 29–31.M. KimuraT. FujitaY. Itokawa1982Liquid-chromatographic determination of the total thiamin content of blood.Clin Chem282931
- 64. Rindi G, Patrini C, Poloni M (1981) Monophosphate, the only phosphoric ester of thiamin in the cerebro-spinal fluid. Experientia 37: 975–976.G. RindiC. PatriniM. Poloni1981Monophosphate, the only phosphoric ester of thiamin in the cerebro-spinal fluid.Experientia37975976
- 65. Wang Y, Zhao R, Russell RG, Goldman ID (2001) Localization of the murine reduced folate carrier as assessed by immunohistochemical analysis. Biochim Biophys Acta 1513: 49–54.Y. WangR. ZhaoRG RussellID Goldman2001Localization of the murine reduced folate carrier as assessed by immunohistochemical analysis.Biochim Biophys Acta15134954
- 66. Zhao R, Gao F, Goldman ID (2002) Reduced folate carrier transports thiamine monophosphate: an alternative route for thiamine delivery into mammalian cells. Am J Physiol Cell Physiol 282: C1512–C1517.R. ZhaoF. GaoID Goldman2002Reduced folate carrier transports thiamine monophosphate: an alternative route for thiamine delivery into mammalian cells.Am J Physiol Cell Physiol282C1512C1517
- 67. Bettendorff L, Wins P, Lesourd M (1994) Subcellular localization and compartmentation of thiamine derivatives in rat brain. Biochim Biophys Acta 1222: 1–6.L. BettendorffP. WinsM. Lesourd1994Subcellular localization and compartmentation of thiamine derivatives in rat brain.Biochim Biophys Acta122216
- 68. Saccà L (2009) Heart failure as a multiple hormonal deficiency syndrome. Circ Heart Fail 2: 151–156.L. Saccà2009Heart failure as a multiple hormonal deficiency syndrome.Circ Heart Fail2151156
- 69. Chaggar PS, Malkin CJ, Shaw SM, Williams SG, Channer KS (2009) Neuroendocrine effects on the heart and targets for therapeutic manipulation in heart failure. Cardiovasc Ther 27: 187–193.PS ChaggarCJ MalkinSM ShawSG WilliamsKS Channer2009Neuroendocrine effects on the heart and targets for therapeutic manipulation in heart failure.Cardiovasc Ther27187193
- 70. Shin BH, Choi SH, Cho EY, Shin MJ, Hwang KC, et al. (2004) Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes. Mol Cells 18: 133–140.BH ShinSH ChoiEY ChoMJ ShinKC Hwang2004Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes.Mol Cells18133140
- 71. Gadau S, Emanueli C, Van Linthout S, Graiani G, Todaro M, et al. (2006) Benfotiamine accelerates the healing of ischaemic diabetic limbs in mice through protein kinase B/Akt-mediated potentiation of angiogenesis and inhibition of apoptosis. Diabetologia 49: 405–420.S. GadauC. EmanueliS. Van LinthoutG. GraianiM. Todaro2006Benfotiamine accelerates the healing of ischaemic diabetic limbs in mice through protein kinase B/Akt-mediated potentiation of angiogenesis and inhibition of apoptosis.Diabetologia49405420
- 72. Klooster A, Leuvenink HG, Gans RO, Bakker SJ (2007) Tissue thiamine deficiency as potential cause of delayed graft function after kidney transplantation: Thiamine supplementation of kidney donors may improve transplantation outcome. Med Hypotheses 69: 873–878.A. KloosterHG LeuveninkRO GansSJ Bakker2007Tissue thiamine deficiency as potential cause of delayed graft function after kidney transplantation: Thiamine supplementation of kidney donors may improve transplantation outcome.Med Hypotheses69873878
- 73. Zempleni J, Link G, Kübler W (1992) The transport of thiamine, riboflavin and pyridoxal 5′-phosphate by human placenta. Int J Vitam Nutr Res 62: 165–172.J. ZempleniG. LinkW. Kübler1992The transport of thiamine, riboflavin and pyridoxal 5′-phosphate by human placenta.Int J Vitam Nutr Res62165172
- 74. Yamazaki M, Hayaishi O (1968) Allosteric properties of nucleoside diphosphatase and its identity with thiamine pyrophosphatase. J Biol Chem 243: 2934–2942.M. YamazakiO. Hayaishi1968Allosteric properties of nucleoside diphosphatase and its identity with thiamine pyrophosphatase.J Biol Chem24329342942
- 75. Sano S, Matsuda Y, Nakagawa H (1988) Type B nucleoside-diphosphatase of rat brain. Purification and properties of an enzyme with high thiamin pyrophosphatase activity. Eur J Biochem 171: 231–236.S. SanoY. MatsudaH. Nakagawa1988Type B nucleoside-diphosphatase of rat brain. Purification and properties of an enzyme with high thiamin pyrophosphatase activity.Eur J Biochem171231236
- 76. Oishi K, Barchi M, Au AC, Gelb BD, Diaz GA (2004) Male infertility due to germ cell apoptosis in mice lacking the thiamin carrier, Tht1. A new insight into the critical role of thiamin in spermatogenesis. Dev Biol 266: 299–309.K. OishiM. BarchiAC AuBD GelbGA Diaz2004Male infertility due to germ cell apoptosis in mice lacking the thiamin carrier, Tht1. A new insight into the critical role of thiamin in spermatogenesis.Dev Biol266299309
- 77. Auger J, Ronot X, Dadoune JP (1989) Human sperm mitochondrial function related to motility: a flow and image cytometric assessment. J Androl 10: 439–448.J. AugerX. RonotJP Dadoune1989Human sperm mitochondrial function related to motility: a flow and image cytometric assessment.J Androl10439448
- 78. Nascimento JM, Shi LZ, Tam J, Chandsawangbhuwana C, Durrant B, et al. (2008) Comparison of glycolysis and oxidative phosphorylation as energy sources for mammalian sperm motility, using the combination of fluorescence imaging, laser tweezers, and real-time automated tracking and trapping. J Cell Physiol 217: 745–751.JM NascimentoLZ ShiJ. TamC. ChandsawangbhuwanaB. Durrant2008Comparison of glycolysis and oxidative phosphorylation as energy sources for mammalian sperm motility, using the combination of fluorescence imaging, laser tweezers, and real-time automated tracking and trapping.J Cell Physiol217745751
- 79. Williams AC, Ford WC (2004) Functional significance of the pentose phosphate pathway and glutathione reductase in the antioxidant defenses of human sperm. Biol Reprod 71: 1309–1316.AC WilliamsWC Ford2004Functional significance of the pentose phosphate pathway and glutathione reductase in the antioxidant defenses of human sperm.Biol Reprod7113091316
- 80. Egi Y, Koyama S, Shikata H, Yamada K, Kawasaki T (1986) Content of thiamin phosphate esters in mammalian tissues - an extremely high concentration of thiamin triphosphate in pig skeletal muscle. Biochem Int 12: 385–390.Y. EgiS. KoyamaH. ShikataK. YamadaT. Kawasaki1986Content of thiamin phosphate esters in mammalian tissues - an extremely high concentration of thiamin triphosphate in pig skeletal muscle.Biochem Int12385390
- 81. Szyniarowski P, Lakaye B, Czerniecki J, Makarchikov AF, Wins P, et al. (2005) Pig tissues express a catalytically inefficient 25-kDa thiamine triphosphatase: insight in the catalytic mechanisms of this enzyme. Biochim Biophys Acta 1725: 93–102.P. SzyniarowskiB. LakayeJ. CzernieckiAF MakarchikovP. Wins2005Pig tissues express a catalytically inefficient 25-kDa thiamine triphosphatase: insight in the catalytic mechanisms of this enzyme.Biochim Biophys Acta172593102
- 82. Lakaye B, Makarchikov AF, Wins P, Margineanu I, Roland S, et al. (2004) Human recombinant thiamine triphosphatase: purification, secondary structure and catalytic properties. Int J Biochem Cell Biol 36: 1348–1364.B. LakayeAF MakarchikovP. WinsI. MargineanuS. Roland2004Human recombinant thiamine triphosphatase: purification, secondary structure and catalytic properties.Int J Biochem Cell Biol3613481364
- 83. De Caro L, Rindi G, De Giuseppe L (1961) Contents in rat tissue of thiamine and its phosphates during dietary thiamine deficiency. Int Z Vitaminforsch 31: 333–340.L. De CaroG. RindiL. De Giuseppe1961Contents in rat tissue of thiamine and its phosphates during dietary thiamine deficiency.Int Z Vitaminforsch31333340
- 84. Balaghi M, Pearson WN (1966) Tissue and intracellular distribution of radioactive thiamine in normal and thiamine-deficient rats. J Nutr 89: 127–132.M. BalaghiWN Pearson1966Tissue and intracellular distribution of radioactive thiamine in normal and thiamine-deficient rats.J Nutr89127132
- 85. Fukuwatari T, Yoshida E, Takahashi K, Shibata K (2010) Effect of fasting on the urinary excretion of water-soluble vitamins in humans and rats. J Nutr Sci Vitaminol (Tokyo) 56: 19–26.T. FukuwatariE. YoshidaK. TakahashiK. Shibata2010Effect of fasting on the urinary excretion of water-soluble vitamins in humans and rats.J Nutr Sci Vitaminol (Tokyo)561926
- 86. Carpenter KJ (2000) Beriberi, white rice, and vitamin B: a disease, a cause, and a cure. Berkeley, CA: University of California Press. KJ Carpenter2000Beriberi, white rice, and vitamin B: a disease, a cause, and a cure.Berkeley, CAUniversity of California Press
- 87. McCandless DW, Schenker S, Cook M (1968) Encephalopathy of thiamine deficiency: studies of intracerebral mechanisms. J Clin Invest 47: 2268–2280.DW McCandlessS. SchenkerM. Cook1968Encephalopathy of thiamine deficiency: studies of intracerebral mechanisms.J Clin Invest4722682280
- 88. Williams RD, Mason HL, Wilder RM, Smith BF (1940) Observations on induced thiamine (vitamin B1) deficiency in Man. Arch Intern Med 66: 785–799.RD WilliamsHL MasonRM WilderBF Smith1940Observations on induced thiamine (vitamin B1) deficiency in Man.Arch Intern Med66785799
- 89. Dreyfus PM (1961) The quantitative histochemical distribution of thiamine in deficient rat brain. J Neurochem 8: 139–145.PM Dreyfus1961The quantitative histochemical distribution of thiamine in deficient rat brain.J Neurochem8139145
- 90. Pincus JH, Grove I (1970) Distribution of thiamine phosphate esters in normal and thiamine-deficient brain. Exp Neurol 28: 477–483.JH PincusI. Grove1970Distribution of thiamine phosphate esters in normal and thiamine-deficient brain.Exp Neurol28477483
- 91. Hazell AS, Butterworth RF (2009) Update of cell damage mechanisms in thiamine deficiency: focus on oxidative stress, excitotoxicity and inflammation. Alcohol Alcohol 44: 141–147.AS HazellRF Butterworth2009Update of cell damage mechanisms in thiamine deficiency: focus on oxidative stress, excitotoxicity and inflammation.Alcohol Alcohol44141147
- 92. Benton D, Griffiths R, Haller J (1997) Thiamine supplementation mood and cognitive functioning. Psychopharmacology (Berl) 129: 66–71.D. BentonR. GriffithsJ. Haller1997Thiamine supplementation mood and cognitive functioning.Psychopharmacology (Berl)1296671
- 93. Hoorn RK, Flikweert JP, Westerink D (1975) Vitamin B-1, B-2 and B-6 deficiencies in geriatric patients, measured by coenzyme stimulation of enzyme activities. Clin Chim Acta 61: 151–162.RK HoornJP FlikweertD. Westerink1975Vitamin B-1, B-2 and B-6 deficiencies in geriatric patients, measured by coenzyme stimulation of enzyme activities.Clin Chim Acta61151162
- 94. Iber FL, Blass JP, Brin M, Leevy CM (1982) Thiamin in the elderly — relation to alcoholism and to neurological degenerative disease. Am J Clin Nutr 36: 1067–1082.FL IberJP BlassM. BrinCM Leevy1982Thiamin in the elderly — relation to alcoholism and to neurological degenerative disease.Am J Clin Nutr3610671082
- 95. Gastaldi G, Laforenza U, Ferrari G, Casasco A, Rindi G (1992) Age-related thiamin transport by small intestinal microvillous vesicles of rat. Biochim Biophys Acta 1105: 271–277.G. GastaldiU. LaforenzaG. FerrariA. CasascoG. Rindi1992Age-related thiamin transport by small intestinal microvillous vesicles of rat.Biochim Biophys Acta1105271277
- 96. Pekovich SR, Poggi V, Martin PR, Singleton CK (1998) Sensitivity to Thiamine Deficiency in Cultured Human Cells is Dependent on Cell Type and is Enhanced in Cells From Thiamine-Responsive Megaloblastic Anemia Patients. J Nutr Biochem 9: 215–222.SR PekovichV. PoggiPR MartinCK Singleton1998Sensitivity to Thiamine Deficiency in Cultured Human Cells is Dependent on Cell Type and is Enhanced in Cells From Thiamine-Responsive Megaloblastic Anemia Patients.J Nutr Biochem9215222
- 97. Gioda CR, de Oliveira Barreto T, Gomes TN, de Lima D, Campos PP, et al. (2010) Cardiac oxidative stress is involved in heart failure induced by thiamine deprivation in rats. Am J Physiol Heart Circ Physiol 298: H2039–H2045.CR GiodaT. de Oliveira BarretoTN GomesD. de LimaPP Campos2010Cardiac oxidative stress is involved in heart failure induced by thiamine deprivation in rats.Am J Physiol Heart Circ Physiol298H2039H2045
- 98. Gioda CR, Roman-Campos D, Carneiro-Junior MA, da Silva KA, de Souza MO, et al. (2009) Impaired cellular contractile function in thiamine-deficient rat cardiomyocytes. Eur J Heart Fail 11: 1126–1128.CR GiodaD. Roman-CamposMA Carneiro-JuniorKA da SilvaMO de Souza2009Impaired cellular contractile function in thiamine-deficient rat cardiomyocytes.Eur J Heart Fail1111261128
- 99. Matsuda T, Doi T, Tonomura H, Baba A, Iwata H (1989) Postnatal development of thiamine metabolism in rat brain. J Neurochem 52: 842–846.T. MatsudaT. DoiH. TonomuraA. BabaH. Iwata1989Postnatal development of thiamine metabolism in rat brain.J Neurochem52842846
- 100. Bettendorff L, Nghiêm HO, Wins P, Lakaye B (2003) A general method for the chemical synthesis of gamma-32P-labeled or unlabeled nucleoside 5(')-triphosphates and thiamine triphosphate. Anal Biochem 322: 190–197.L. BettendorffHO NghiêmP. WinsB. Lakaye2003A general method for the chemical synthesis of gamma-32P-labeled or unlabeled nucleoside 5(')-triphosphates and thiamine triphosphate.Anal Biochem322190197
- 101. Hinting A, Schoonjans F, Comhaire F (1988) Validation of a single-step procedure for the objective assessment of sperm motility characteristics. Int J Androl 11: 277–287.A. HintingF. SchoonjansF. Comhaire1988Validation of a single-step procedure for the objective assessment of sperm motility characteristics.Int J Androl11277287
- 102. Krüger TF, Acosta AA, Simons KF, Swanson RJ, Matta JF, et al. (1988) Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril 49: 112–117.TF KrügerAA AcostaKF SimonsRJ SwansonJF Matta1988Predictive value of abnormal sperm morphology in in vitro fertilization.Fertil Steril49112117
- 103. Bettendorff L, Peeters M, Jouan C, Wins P, Schoffeniels E (1991) Determination of thiamin and its phosphate esters in cultured neurons and astrocytes using an ion-pair reversed-phase high-performance liquid chromatographic method. Anal Biochem 198: 52–59.L. BettendorffM. PeetersC. JouanP. WinsE. Schoffeniels1991Determination of thiamin and its phosphate esters in cultured neurons and astrocytes using an ion-pair reversed-phase high-performance liquid chromatographic method.Anal Biochem1985259
- 104. Peterson GL (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83: 346–356.GL Peterson1977A simplification of the protein assay method of Lowry et al. which is more generally applicable.Anal Biochem83346356