Chronic psychological stress is associated with detrimental effects on physical health, and may operate in part through accelerated cell aging, as indexed by shorter telomeres at the ends of chromosomes. However, not all people under stress have distinctly short telomeres, and we examined whether exercise can serve a stress-buffering function. We predicted that chronic stress would be related to short telomere length (TL) in sedentary individuals, whereas in those who exercise, stress would not have measurable effects on telomere shortening.
Methodology and Principal Findings
63 healthy post-menopausal women underwent a fasting morning blood draw for whole blood TL analysis by a quantitative polymerase chain reaction method. Participants completed the Perceived Stress Scale (Cohen et al., 1983), and for three successive days reported daily minutes of vigorous activity. Participants were categorized into two groups-sedentary and active (those getting Centers for Disease Control-recommended daily amount of activity). The likelihood of having short versus long telomeres was calculated as a function of stress and exercise group, covarying age, BMI and education. Logistic regression analyses revealed a significant moderating effect of exercise. As predicted, among non-exercisers a one unit increase in the Perceived Stress Scale was related to a 15-fold increase in the odds of having short telomeres (p<.05), whereas in exercisers, perceived stress appears to be unrelated to TL (B = −.59, SE = .78, p = .45).
Citation: Puterman E, Lin J, Blackburn E, O'Donovan A, Adler N, Epel E (2010) The Power of Exercise: Buffering the Effect of Chronic Stress on Telomere Length. PLoS ONE 5(5): e10837. https://doi.org/10.1371/journal.pone.0010837
Editor: Jose Vina, University of Valencia, Spain
Received: January 28, 2010; Accepted: April 30, 2010; Published: May 26, 2010
Copyright: © 2010 Puterman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The research study was supported by the Division of Behavioral and Social Research at the National Institute of Aging/National Institutes of Health R56 grant (Epel) and Bernard and Barbro Foundation (Blackburn). The Gladstone flow core and the Core Immunology Lab were supported by the UCSF-GIVI Center for AIDS Research P30AI027763. The CTSI CCRC and the Core Immunology Lab were supported by NIH/NCRR UCSF-CTSI grant number UL1 RR024131. The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: EB, EE, and JL are co-founders of a diagnostics company related to telomere biology, and own stock in the company.
Chronic psychological stress can have a detrimental impact on human physical health, . Unemployment, financial strain, caregiving for a chronically ill loved one, and other stressful life experiences have been related to increased risks for cardiovascular disease, insulin resistance, and other markers of disease. In the past half-decade, accumulating evidence suggests that one of the pathways through which chronic stress may impact health is through accelerated cell aging, as indexed by the length of the telomeric DNA at the end of chromosomes–. Telomeres are DNA-protein complexes that cap chromosomal ends, promoting chromosomal stability. At each cellular division, telomeric DNA terminal regions are not fully replicated, which, if not counteracted by elongation by telomerase, can lead to telomere shortening. If telomeres shorten to a critical length, cells cease to multiply, often by entering a state of senescence characterized by, for example, increased cellular secretion of proinflammatory cytokines and loss of antigen recognition in the case of immune system cells. As a result, telomere length (TL) has emerged as a widely recognized biomarker of immune cell biological ‘age’. Short TL in white blood cells has been linked to a range of health problems, including coronary heart disease, and diabetes mellitus, and to early mortality–.
In the first study to link psychological stress with short TL, it was demonstrated that among pre-menopausal women caregiving for a chronically ill child, duration of caregiving was associated with short TL. Moreover, the results were not simply a result of caregiving, because in addition, greater perceived stress was associated with shorter TL consistently across the entire sample, which included control mothers of ‘healthy’ children. Yet, not all people under chronic stress develop health problems, and likely, do not have distinctly short telomeres, although no studies have yet identified potential moderators of this relationship.
Several studies have examined health behaviors as potential mediators through which stress affects health, but few have examined their potential to ameliorate the effects of stress on physiology. Here we propose that a specific health behavior, physical activity, can moderate the impact of stress on cell aging. Physical activity is related to longer telomeres, both in a national sample of twins, and in endurance trained athletes compared to sedentary controls. Therefore, we hypothesized that stressed yet physically active adults will be more protected from the impact of stress on their TL compared to those stressed and not active. Specifically, we predicted that chronic stress will be related to short telomeres only in those inactive (defined as not engaging in recommended amounts of physical activity across a three day period) while physically active adults will show no association between stress and TL. We examined these associations and the protective capacity of exercise in 63 post-menopausal healthy, non-smoking women.
Table 1 presents means and standard deviations for perceived stress, physical activity, and TL and covariates. Participants with higher perceived stress were significantly less likely to exercise (r (61) = −.27, p = .03), had shorter TL (r (57) = −.29, p = .03), higher BMI (r (61) = .26, p = .04) and fewer years of education (r (61) = −.27, = .03). Across the sample as a whole, minutes of physical activity and TL were unrelated to each other (r (58) = .04, p = .74), and neither was related to any of the potential covariates described above.
Next, we examined whether physical activity (1 = meets or exceeds recommended amount, active vs. 0 = does not meet recommendation, sedentary) moderated the stress-TL relationship. In a logistic multiple regression analysis, we compared a dichotomous telomere outcome, the two extreme TL groups: those in the highest (n = 20) and lowest (n = 21) tertiles of TL. We predicted the likelihood of having short telomeres as a function of the direct effects of stress and exercise group, and the interaction effect of the two groups as well. Covarying education, and BMI, the likelihood of having short telomeres was greater with higher levels of perceived stress (p<.05), however this relationship was significantly moderated by exercise category (interaction b = −3.32, SE = 1.40, p = .02). Given that age,  and antioxidant use have been associated with TL in past studies, we repeated all analyses including these two variables as covariates. Results were similar even after including age and antioxidant vitamin intake (interaction b = −3.34, SE = 1.38, p = .02). For those who exercised less than the recommended amount, a one unit increase in the perceived stress scale mean item response was related to a 15-fold increase in the odds of having short telomeres (B0 = −4.27, Bstress = 2.74, SEstress = 1.09, OR = 15.48, p = .01), controlling for mean-centered education and BMI. On the other hand, perceived stress appeared to have little effect on telomere category (short versus long) in those participants who exercised the recommended amount over a three day period (B0 = .71, Bstress = −.59, SEstress = .78, OR = .56, p = .45), controlling for mean-centered education and BMI. Figure 1 illustrates the fitted probability (from 0 to 1) of being categorized as having short telomeres as a function of perceived stress for inactive versus active participants. Probability scores were fitted from each model from the logistic regression equation, at mean BMI and education level. Further, for illustrative purposes, we present a scatter-plot graph (Figure 2) that predicts the entire sample of TL as a function of perceived stress for active and inactive participants. As seen in Figure 2, higher levels of perceived stress significantly predict decreased TL in inactive participants only.
Note. Physical activity categories are based on whether the participant met CDC recommended levels of exercise per week. Perceived stress ratings are based on the Perceived Stress Scale. The interaction effect was significant (p<.05), indicating that the relationship between perceived stress and telomere length was significant in inactive participants only. The Y axis probability presents the probability of categorization into short telomere length (bottom tertile) as a function of perceived stress in inactive (top of figure) versus active (bottom of figure) participants. Probability scores were calculated from the fitted regression equations, assuming mean BMI and education level.
Note. Physical activity categories are based on whether the participant met CDC recommended levels of exercise per week. Perceived stress ratings are based on the Perceived Stress Scale. The relationship between perceived stress and telomere length was significant in sedentary participants only.
As a secondary analysis, to more sensitively test the level of exercise that matters most, we employed the Johnson-Neyman statistical approach to examine the significant confidence intervals at which reported minutes of physical activity protected participants from the stress effects on TL. At values of exercise below 42 minutes across the three days, perceived stress was significantly related to the odds of having short TL. On the other hand, at values higher than 42 minutes, perceived stress was unrelated to TL (see Table 2). Thus, at least 14 minutes a day of vigorous exercise appears to be a critical amount for protection from the effects of stress.
Lastly, recent evidence suggests that female estrogen-deficient mice express reduced telomerase activity, and that hormone replacement (HR) restores the levels to those of control mice. In light of these findings, we reran the above analyses removing the two participants in our sample who were undergoing HR therapy. Results were similar with and without these participants and thus results presented contain all participants.
Consistent with previous studies, , , our data indicate that higher perceived psychological stress is associated with shorter TL. Importantly, physical activity was able to protect those experiencing stress by buffering its relationship with TL, as hypothesized. Our findings support the physical benefits of activity, and, interestingly, map well onto Centers for Disease Control (CDC) recommendations for amount of physical activity required to sustain a healthy body (75 minutes of vigorous exercise per week). Furthermore, physical activity buffered the impact of stress on TL even after covarying for factors clearly associated with stress, physical activity or telomere—education, age, , body mass index, and antioxidant vitamin use, . For example, lower socioeconomic status individuals are more likely to experience stress and engage in less leisure time physical activity. Physically unfit and stressed individuals are often more at risk for obesity and other health problems, . In the present study, we were able to rule out these potential factors as alternative explanations for both the stress-telomere association and the buffering potential of physical activity.
The mechanisms by which stress impacts the body at the cellular level are unknown, but one likely pathway is telomerase activity. Telomerase is a cellular ribonucleoprotein reverse transcriptase enzyme that adds telomeric DNA to shortened telomeres, and protects them. Chronically stressed individuals have lower telomerase activity and often demonstrate altered glucocorticoid profiles. Higher excretion of urinary stress hormones is cross sectionally related to lower telomerase and shorter telomeres, . Further, a recent experimental study demonstrated that exposing human lymphocytes, in vitro, to high levels of cortisol during primary stimulation and upon subsequent re-stimulation can dampen telomerase activity. Autonomic nervous system profiles associated with chronic stress (i.e. high blood pressure, lower heart rate variability) are also associated with lower telomerase activity.
Physical activity training augments telomerase activity in myocytes and circulating mononuclear cells in rats, , and in human leukocytes , thus providing a plausible and potentially testable pathway to explain our findings. Exercise might also buffer telomere shortening through affecting the balance between oxidative stress and antioxidants. Chronic stress has been linked to increased oxidative stress, , and is known to shorten telomeres and inhibit telomerase activity in cells in vitro , . Aerobic training interventions can enhance endogenous antioxidant activity in human and animal models, , although not all studies on this have shown consistent findings. While vigorous physical activity is paradoxically linked to increased oxidative damage, exercisers nevertheless have lengthened telomeres. Recent models of exercise propose that physical activity promotes a ‘eustress’ state, whereby exercise, at moderate levels, promotes manageable and beneficial effects. On the other hand, extreme exercise promotes catabolic processes and ultimate cell damage rather than strengthening .
Furthermore, physical activity may act to protect highly stressed individuals through autonomic, neuroendocrine and cognitive pathways. Sympathetic nervous system and cortisol activation in response to stress is often blunted in physically fit humans and other animals–. Heart rate variability is improved in response to fitness training. Cognitive responses to stress, such as rumination, are associated with enhanced cortisol responses to stress. Exercise induction, which leads to decreased rumination across time and enhanced feelings of self-efficacy, may reduce the cognitive processing thought to activate neuroendocrine and autonomic responses to stress.
In the present study we also examined the interval of physical activity above which stress no longer significantly predicts TL. The data indicated that above around 40 minutes of vigorous activity over 3 days, stress is no longer associated with short telomeres. Forty minutes over 3 days correspond well with the CDC-recommended levels of vigorous activity, as well as those recommended by others for healthy living, . An assumption in the present study was that a 3-day accumulation of reported physical activity represented our participants' typical engagement of exercise, and a long-term lifestyle factor. Future studies should be designed to measure participants' baseline fitness as well as daily reported engagement in activity for longer durations. These studies could better delineate the number of weekly minutes of exercise required to protect stressed individuals, and to observe actual benefits of physical activity at the cellular level.
The findings of the present study underscore an inherent problem in promoting physical activity in stressed individuals: while physical activity may confer more benefits to highly stressed individuals, they are simply less likely to actually exercise. It will be helpful to better understand the role of contextual factors (such as personality, motivation, and daily events) that promote physical activity in stressed versus non-stressed individuals. In contrast to previous studies on physically fit adults and telomeres, , we did not demonstrate direct associations between physical activity and TL across our entire study sample. Work by Cherkas and colleagues demonstrating a relationship between physical exercise and longer TL used a larger sample of adults (N>2500) with a wider age range of participants and physical activity categories. Our smaller sample size, restricted age range, and definition of physical activity may explain our divergent finding, although further examination of this relationship with a larger sample size is required.
In summary, the novel findings reported here support the buffering potential of physical activity on the detrimental effects of stress on cellular longevity. While we ruled out several lifestyle factors previously linked to TL (e.g., BMI, vitamin intake), the possibility remains that other health promoting behaviors may also have similar buffering effects, or that other factors associated with both exercise and TL may be at work. Intervention studies are still needed to provide a better test of this relationship. Intervention studies have already shown that exercise can improve major depression, which is another sequela of chronic stress, . It is reasonable to conclude that exercise should be strongly advised and prescribed to people reporting high levels of psychological stress.
Materials and Methods
Participants and procedure
Sixty-three healthy post-menopausal women aged between 54 and 82 years were recruited through flyers and posters in the community, and from service providers serving the elderly in the San Francisco Bay Area. They were part of a prospective study on caregiving and its effects on physical and psychological well being. Women were recruited specifically for having varying levels of stress levels, and so included both dementia caregivers and non-caregivers, each showing a range of stress levels but with higher levels in the caregivers. The sample described here included 50 White (79.7%), three Black, one Hispanic/Latina, six Asian women, and two women of Asian and White background. Seventy percent of participants had at least a Bachelor's degree, and the average household income fell between $60,000 and $69,000. Exclusion criteria included the presence of major medical conditions such as heart disease, cancer, or diabetes, use of medications containing agents known to affect stress hormone levels, and regular smoking. In a fasted state, participants had a catheter insertion, and after resting for an hour, they underwent a blood draw between 8.00 AM and 10.00 AM, from the non-dominant arm. Participants completed self-report questionnaires and exercise logs within one week of the blood draw. The study protocol was approved by the Institutional Review Board of the University of California, San Francisco. Written, informed consent was obtained from all participants.
Materials and measures
The 10-item Perceived Stress Scale was used to assess appraisals of psychological stress experienced during the last month, including the extent to which situations are experienced as unpredictable, uncontrollable and overwhelming. Participants are asked to rate the extent to which they felt or thought a particular way in the previous month on a 5-point Likert scale ranging from 0 (“never”) to 4 (“very often”). Internal consistency was high (a = .93).
Participants reported on a daily basis for three successive days their amount of vigorous exercise, defined as “increased heart rate and/or sweating.” Higher levels of self-reported physical activity are associated with greater fitness, as measured by the metabolic equivalent (MET). Retrospective reporting is plagued by recall bias, and thus daily reporting of behaviors is considered a stronger measure of behavior, . Daily reports of physical activity were extremely skewed (range 0 to 300 minutes, 30 participants reported no exercise), and therefore we split participants based on previously reported required amounts of physical activity for good health. The Center for Disease Control and Prevention, based on previous work, recommends an average of 75 minutes of vigorous activity per week (an average of 33 minutes for a three day period). We thus split participants based on meeting these recommended guidelines (less than 33 minutes = 0, equal or greater than 33 minutes = 1), labeled here, for brevity, as “sedentary” vs. “active”. We also report physical activity as a continuous measure for our means, standards deviations, correlations and t-tests.
Telomere length (TL).
Samples were collected in 10-ml heparin tubes (Becton–Dickinson, Franklin Lakes, NJ). Leukocytes were isolated and frozen at −80°C. DNA was extracted from leukocytes by the University of California San Francisco DNA bank. Genomic DNA isolation was performed using a standardized and quality-controlled PureGene DNA isolation system (Gentra Systems, Minneapolis). The quantity and quality of the genomic DNA isolate was determined by 260/280 UV spectrophotometery. At regular intervals, the integrity of isolated DNA was evaluated by agarose gel electrophoresis performed on randomly selected isolates.
DNA was analyzed for TL using quantitative polymerase chain reaction (qPCR) as previously described with the following modifications. The primers for the telomere PCR were tel1b [5′-CGGTTT(GTTTGG)5GTT-3′], used at a final concentration of 100 nM, and tel2b [5′-GGCTTG(CCTTAC)5CCT-3′], used at a final concentration of 900 nM. The primers for the single-copy gene (human beta-globin) PCR were hbg1 [5′ GCTTCTGACACAACTGTGTTCACTAGC-3′], used at a final concentration of 300 nM, and hbg2 [5′-CACCAACTTCATCCACGTTCACC-3′], used at a final concentration of 700 nM. Serial dilutions of genomic DNA from the Hela cancer cell line were used as the reference DNA to create the standard curve. The quantities of telomeric product (T) and single copy gene (S) were determined relative to the reference DNA by the standard curve method. All PCRs were carried out on a Roche Lightcycler 480 real-time PCR machine with 384-tube capacity (Roche Diagnostics Corporation, Indianapolis, IN). The telomere thermal cycling profile consists of: cycling for T (telomeric) PCR: denature at 96°C for 1 second, anneal/extend at 54°C for 60 seconds, with fluorescence data collection, 30 cycles; cycling for S (single copy gene) PCR: denature at 95°C for 15 seconds, anneal at 58°C for 1 second, extend at 72°C for 20 seconds, 8 cycles; followed by denature at 96°C for 1 second, anneal at 58°C for 1 second, extend at 72°C for 20 seconds, hold at 83°C for 5 seconds with data collection, 35 cycles. Details of the TL measurement method are described elsewhere.
Covariates examined were relevant factors known to be associated with TL in previous studies. In the present study we examined age in years, education (1 = less than 12 years, 2 = high school graduate, 3 = some college or technical school, 4 = AA degree, 5 = bachelor's degree, 6 = advanced degree), anti-oxidant vitamin intake (binary coded; 48 reported taking anti-oxidant vitamins and 15 reported no intake) and body mass index (BMI). BMI was calculated as weight in kilograms (measured on a balance beam scale in hospital gown) divided by height in meters squared. To maintain power, we retained only the covariates that were related to any of our predictors or outcome.
We thank the study participants for their support and generous contribution of time. We thank Dr. Richard Cawthon for his technical advice on the telomere length measurement method.
Conceived and designed the experiments: EB NA EE. Performed the experiments: JL AO EE. Analyzed the data: EP. Contributed reagents/materials/analysis tools: JL EB EE. Wrote the paper: EP JL EB AO NA EE.
- 1. McEwen BS (2004) Protection and damage from acute and chronic stress. Ann NY Acad Sci 1032: 1–7.BS McEwen2004Protection and damage from acute and chronic stress.Ann NY Acad Sci103217
- 2. Kiecolt-Glaser JK, Glaser R (1999) Chronic stress and mortality among older adults. JAMA 282(23): 2259.JK Kiecolt-GlaserR. Glaser1999Chronic stress and mortality among older adults.JAMA282(23)2259
- 3. Janicki-Deverts D, Cohen S, Matthews KA, Cullen MR (2008) History of unemployment predicts future elevations in C-reactive protein among male participants in the Coronary Artery Risk Development in Young Adults (CARDIA) Study. Annals of Behavioral Medicine 36(2): 176–185.D. Janicki-DevertsS. CohenKA MatthewsMR Cullen2008History of unemployment predicts future elevations in C-reactive protein among male participants in the Coronary Artery Risk Development in Young Adults (CARDIA) Study.Annals of Behavioral Medicine36(2)176185
- 4. Steptoe A, Brydon L, Kunz-Ebrecht S (2005) Changes in Financial Strain Over Three Years, Ambulatory Blood Pressure, and Cortisol Responses to Awakening. Psychosom Med 67(2): 281–287.A. SteptoeL. BrydonS. Kunz-Ebrecht2005Changes in Financial Strain Over Three Years, Ambulatory Blood Pressure, and Cortisol Responses to Awakening.Psychosom Med67(2)281287
- 5. Kiecolt-Glaser JK, Glaser R, Shuttleworth EC, Dyer CS, Ogrocki P, et al. (1987) Chronic stress and immunity in family caregivers of Alzheimer's disease victims. Psychosom Med 49(5): 523.JK Kiecolt-GlaserR. GlaserEC ShuttleworthCS DyerP. Ogrocki1987Chronic stress and immunity in family caregivers of Alzheimer's disease victims.Psychosom Med49(5)523
- 6. Mooy JM, De Vries H, Grootenhuis PA, Bouter LM, Heine RJ (2000) Major stressful life events in relation to prevalence of undetected type 2 diabetes. Diabetes Care 23(2): 197–201.JM MooyH. De VriesPA GrootenhuisLM BouterRJ Heine2000Major stressful life events in relation to prevalence of undetected type 2 diabetes.Diabetes Care23(2)197201
- 7. Epel ES, Blackburn EH, Lin J, Dhabhar FS, Adler NE, et al. (2004) Accelerated telomere shortening in response to life stress. Proceedings of the National Academy of Sciences 101(49): 17312–17315.ES EpelEH BlackburnJ. LinFS DhabharNE Adler2004Accelerated telomere shortening in response to life stress.Proceedings of the National Academy of Sciences101(49)1731217315
- 8. Epel ES, Lin J, Wilhelm FH, Wolkowitz OM, Cawthon R, et al. (2006) Cell aging in relation to stress arousal and cardiovascular disease risk factors. Psychoneuroendocrinology 31(3): 277–287.ES EpelJ. LinFH WilhelmOM WolkowitzR. Cawthon2006Cell aging in relation to stress arousal and cardiovascular disease risk factors.Psychoneuroendocrinology31(3)277287
- 9. Parks CG, Miller DB, McCanlies EC, Cawthon RM, Andrew ME, et al. (2009) Telomere length, current perceived stress, and urinary stress hormones in women. Cancer Epidemiology Biomarkers & Prevention 18(2): 551.CG ParksDB MillerEC McCanliesRM CawthonME Andrew2009Telomere length, current perceived stress, and urinary stress hormones in women.Cancer Epidemiology Biomarkers & Prevention18(2)551
- 10. Damjanovic AK, Yang Y, Glaser R, Kiecolt-Glaser JK, Nguyen H, et al. (2007) Accelerated Telomere Erosion Is Associated with a Declining Immune Function of Caregivers of Alzheimer's Disease Patients. J Immunol 179(6): 4249–4254.AK DamjanovicY. YangR. GlaserJK Kiecolt-GlaserH. Nguyen2007Accelerated Telomere Erosion Is Associated with a Declining Immune Function of Caregivers of Alzheimer's Disease Patients.J Immunol179(6)42494254
- 11. Kotrschal A, Ilmonen P, Penn DJ (2007) Stress impacts telomere dynamics. Biology Letters 3(2): 128.A. KotrschalP. IlmonenDJ Penn2007Stress impacts telomere dynamics.Biology Letters3(2)128
- 12. Effros RB, Dagarag M, Spaulding C, Man J (2005) The role of CD8+ T-cell replicative senescence in human aging. Immunological Reviews 205(1): 147–157.RB EffrosM. DagaragC. SpauldingJ. Man2005The role of CD8+ T-cell replicative senescence in human aging.Immunological Reviews205(1)147157
- 13. Blackburn EH (1991) Structure and function of telomeres. Nature 350: 569–573.EH Blackburn1991Structure and function of telomeres.Nature350569573
- 14. Cawthon RM, Smith KR, O'Brien E, Sivatchenko A, Kerber RA (2003) Association between telomere length in blood and mortality in people aged 60 years or older. The Lancet 361(9355): 393–395.RM CawthonKR SmithE. O'BrienA. SivatchenkoRA Kerber2003Association between telomere length in blood and mortality in people aged 60 years or older.The Lancet361(9355)393395
- 15. Demissie S, Levy D, Benjamin EJ, Cupples LA, Gardner JP, et al. (2006) Insulin resistance, oxidative stress, hypertension, and leukocyte telomere length in men from the Framingham Heart Study. Aging Cell 5(4): 325.S. DemissieD. LevyEJ BenjaminLA CupplesJP Gardner2006Insulin resistance, oxidative stress, hypertension, and leukocyte telomere length in men from the Framingham Heart Study.Aging Cell5(4)325
- 16. Fitzpatrick AL, Kronmal RA, Gardner JP, Psaty BM, Jenny NS, et al. (2007) Leukocyte telomere length and cardiovascular disease in the cardiovascular health study. American Journal of Epidemiology 165(1): 14.AL FitzpatrickRA KronmalJP GardnerBM PsatyNS Jenny2007Leukocyte telomere length and cardiovascular disease in the cardiovascular health study.American Journal of Epidemiology165(1)14
- 17. Valdes AM, Andrew T, Gardner JP, Kimura M, Oelsner E, et al. (2005) Obesity, cigarette smoking, and telomere length in women. The Lancet 366(9486): 662–664.AM ValdesT. AndrewJP GardnerM. KimuraE. Oelsner2005Obesity, cigarette smoking, and telomere length in women.The Lancet366(9486)662664
- 18. Cherkas LF, Hunkin JL, Kato BS, Richards JB, Gardner JP, et al. (2008) The association between physical activity in leisure time and leukocyte telomere length. Archives of Internal Medicine 168(2): 154.LF CherkasJL HunkinBS KatoJB RichardsJP Gardner2008The association between physical activity in leisure time and leukocyte telomere length.Archives of Internal Medicine168(2)154
- 19. Werner C, Furster T, Widmann T, Poss J, Roggia C, et al. (2009) Physical Exercise Prevents Cellular Senescence in Circulating Leukocytes and in the Vessel Wall. Circulation 120: 2438–2447.C. WernerT. FursterT. WidmannJ. PossC. Roggia2009Physical Exercise Prevents Cellular Senescence in Circulating Leukocytes and in the Vessel Wall.Circulation12024382447
- 20. Vaziri H, Dragowska W, Allsopp RC, Thomas TE, Harley CB, et al. (1994) Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age. Proceedings of the National Academy of Sciences of the United States of America 91(21): 9857–9860.H. VaziriW. DragowskaRC AllsoppTE ThomasCB Harley1994Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age.Proceedings of the National Academy of Sciences of the United States of America91(21)98579860
- 21. Frenck RW, Blackburn EH, Shannon KM (1998) The rate of telomere sequence loss in human leukocytes varies with age. Proceedings of the National Academy of Sciences of the United States of America 95(10): 5607–5610.RW FrenckEH BlackburnKM Shannon1998The rate of telomere sequence loss in human leukocytes varies with age.Proceedings of the National Academy of Sciences of the United States of America95(10)56075610
- 22. Farzaneh-Far R, Lin J, Epel ES, Harris WS, Blackburn EH, et al. (2010) Association of Marine Omega-3 Fatty Acid Levels With Telomeric Aging in Patients With Coronary Heart Disease. JAMA 303(3): 250.R. Farzaneh-FarJ. LinES EpelWS HarrisEH Blackburn2010Association of Marine Omega-3 Fatty Acid Levels With Telomeric Aging in Patients With Coronary Heart Disease.JAMA303(3)250
- 23. Preacher KJ, Curran PJ, Bauer DJ (2006) Computational tools for probing interactions in multiple linear regression, multilevel modeling, and latent curve analysis. Journal of Educational and Behavioral Statistics 31(4): 437.KJ PreacherPJ CurranDJ Bauer2006Computational tools for probing interactions in multiple linear regression, multilevel modeling, and latent curve analysis.Journal of Educational and Behavioral Statistics31(4)437
- 24. Bayne S, Jones ME, Li H, Pinto AR, Simpson ER, et al. (2008) Estrogen deficiency leads to telomerase inhibition, telomere shortening and reduced cell proliferation in the adrenal gland of mice. Cell Res 18(11): 1141–1150.S. BayneME JonesH. LiAR PintoER Simpson2008Estrogen deficiency leads to telomerase inhibition, telomere shortening and reduced cell proliferation in the adrenal gland of mice.Cell Res18(11)11411150
- 25. Adler N, Snibbe A (2003) The role of psychosocial processes in explaining the gradient between socioeconomic status and health. Current Directions in Psychological Science12(4): 119–123.N. AdlerA. Snibbe2003The role of psychosocial processes in explaining the gradient between socioeconomic status and health.Current Directions in PsychologicalScience12(4)119123
- 26. Richards JB, Valdes AM, Gardner JP, Paximadas D, Kimura M (2007) Higher serum vitamin D concentrations are associated with longer leukocyte telomere length in women. Am J Clin Nutr 86(5): 1420–1425.JB RichardsAM ValdesJP GardnerD. PaximadasM. Kimura2007Higher serum vitamin D concentrations are associated with longer leukocyte telomere length in women.Am J Clin Nutr86(5)14201425
- 27. Ford ES, Merritt RK, Heath GW, Powell KE, Washburn RA, et al. (1991) Physical Activity Behaviors in Lower and Higher Socioeconomic Status Populations. Am J Epidemiol 133(12): 1246–1256.ES FordRK MerrittGW HeathKE PowellRA Washburn1991Physical Activity Behaviors in Lower and Higher Socioeconomic Status Populations. Am J Epidemiol133(12)12461256
- 28. McEwen BS (1998) Protective and damaging effects of stress mediators. The New England Journal of Medicine 338(3): 171.BS McEwen1998Protective and damaging effects of stress mediators.The New England Journal of Medicine338(3)171
- 29. Pate R, Pratt M, Blair S, Haskell W, Macera CA, et al. (1995) Physical Activity and Public Health–A Recommendation from the Centers for Disease Control and Prevention and the American College of Sports Medicine. JAMA 273: 402–407.R. PateM. PrattS. BlairW. HaskellCA Macera1995Physical Activity and Public Health–A Recommendation from the Centers for Disease Control and Prevention and the American College of Sports Medicine.JAMA273402407
- 30. Greider CW, Blackburn EH (1989) A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature 337(6205): 331–337.CW GreiderEH Blackburn1989A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis.Nature337(6205)331337
- 31. Choi J, Fauce SR, Effros RB (2008) Reduced telomerase activity in human T lymphocytes exposed to cortisol. Brain, Behavior, and Immunity 22(4): 600–605.J. ChoiSR FauceRB Effros2008Reduced telomerase activity in human T lymphocytes exposed to cortisol.Brain, Behavior, and Immunity22(4)600605
- 32. Werner C, Hanhoun M, Widmann T, Kazakov A, Semenov A (2008) Effects of Physical Exercise on Myocardial Telomere-Regulating Proteins, Survival Pathways, and Apoptosis. Journal of the American College of Cardiology 52(6): 470–482.C. WernerM. HanhounT. WidmannA. KazakovA. Semenov2008Effects of Physical Exercise on Myocardial Telomere-Regulating Proteins, Survival Pathways, and Apoptosis.Journal of the American College of Cardiology52(6)470482
- 33. Gidron Y, Russ K, Tissarchondou H, Warner J (2006) The relation between psychological factors and DNA-damage: A critical review. Biological Psychology 72(3): 291–304.Y. GidronK. RussH. TissarchondouJ. Warner2006The relation between psychological factors and DNA-damage: A critical review.Biological Psychology72(3)291304
- 34. Finkel T, Holbrook NJ (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408(6809): 239–247.T. FinkelNJ Holbrook2000Oxidants, oxidative stress and the biology of ageing.Nature408(6809)239247
- 35. von Zglinicki T (2002) Oxidative stress shortens telomeres. Trends in Biochemical Sciences 27(7): 339–344.T. von Zglinicki2002Oxidative stress shortens telomeres.Trends in Biochemical Sciences27(7)339344
- 36. Gomez-Cabrera M, Domenech E, Viña J (2008) Moderate exercise is an antioxidant: upregulation of antioxidant genes by training. Free Radic. Biol. Med 44(2): 126–131.M. Gomez-CabreraE. DomenechJ. Viña2008Moderate exercise is an antioxidant: upregulation of antioxidant genes by training. Free Radic. Biol.Med44(2)126131
- 37. Elosua R, Molina L, Fito M, Arquer A, Sanchez-Quesada JL, et al. (2003) Response of oxidative stress biomarkers to a 16-week aerobic physical activity program, and to acute physical activity, in healthy young men and women. Atherosclerosis 167(2): 327–334.R. ElosuaL. MolinaM. FitoA. ArquerJL Sanchez-Quesada2003Response of oxidative stress biomarkers to a 16-week aerobic physical activity program, and to acute physical activity, in healthy young men and women.Atherosclerosis167(2)327334
- 38. Moraska A, Deak T, Spencer RL, Roth D, Fleshner M (2000) Treadmill running produces both positive and negative physiological adaptations in Sprague-Dawley rats. Am J Physiol Regul Integr Comp Physiol 279(4): R1321–1329.A. MoraskaT. DeakRL SpencerD. RothM. Fleshner2000Treadmill running produces both positive and negative physiological adaptations in Sprague-Dawley rats.Am J Physiol Regul Integr Comp Physiol279(4)R13211329
- 39. Alessio HM (1993) Exercise-induced oxidative stress. Medicine & Science in Sports & Exercise 25(2): 218.HM Alessio1993Exercise-induced oxidative stress.Medicine & Science in Sports & Exercise25(2)218
- 40. Li G, He H (2009) Hormesis, allostatic buffering capacity and physiological mechanism of physical activity: A new theoretic framework. Medical Hypotheses 72(5): 527–532.G. LiH. He2009Hormesis, allostatic buffering capacity and physiological mechanism of physical activity: A new theoretic framework.Medical Hypotheses72(5)527532
- 41. Jurca R, Church TS, Morss GM, Jordan AN, Earnest CP (2004) Eight weeks of moderate-intensity exercise training increases heart rate variability in sedentary postmenopausal women. American Heart Journal 147(5): e8–e15.R. JurcaTS ChurchGM MorssAN JordanCP Earnest2004Eight weeks of moderate-intensity exercise training increases heart rate variability in sedentary postmenopausal women.American Heart Journal147(5)e8e15
- 42. Shephard , RJ , Shek , PN (1994) Potential impact of physical activity and sport on the immune system - A brief review. Br J Sports Med 4(28): 247–255.ShephardRJShekPN1994Potential impact of physical activity and sport on the immune system - A brief review.Br J Sports Med4(28)247255
- 43. Traustadóttir T, Bosch PR, Matt KS (2005) The HPA axis response to stress in women: effects of aging and fitness. Psychoneuroendocrinology 2005 May; 30(4): 392–402.T. TraustadóttirPR BoschKS Matt2005The HPA axis response to stress in women: effects of aging and fitness.Psychoneuroendocrinology 2005 May;30(4)392402
- 44. Mueller PJ (2007) Exercise training and sympathetic nervous system activity: evidence for physical activity dependent neural plasticity. Clinical & Experimental Pharmacology & Physiology 34(4): 377.PJ Mueller2007Exercise training and sympathetic nervous system activity: evidence for physical activity dependent neural plasticity.Clinical & Experimental Pharmacology & Physiology34(4)377
- 45. Dishman RK, Berthoud HR, Booth FW, Cotman CW, Edgerton VR, et al. (2006) Neurobiology of exercise. Obesity Research 14(3): 345–356.RK DishmanHR BerthoudFW BoothCW CotmanVR Edgerton2006Neurobiology of exercise.Obesity Research14(3)345356
- 46. Earnest CP, Lavie CJ, Blair SN, Church TS (2008) Heart rate variability characteristics in sedentary postmenopausal women following six months of exercise training: The DREW Study. PLoS ONE 3(6): e2288.CP EarnestCJ LavieSN BlairTS Church2008Heart rate variability characteristics in sedentary postmenopausal women following six months of exercise training: The DREW Study.PLoS ONE3(6)e2288
- 47. Zoccola PM, Dickerson SS, Zaldivar FP (2008) Rumination and cortisol responses to laboratory stressors. Psychosomatic Medicine 70(6): 661.PM ZoccolaSS DickersonFP Zaldivar2008Rumination and cortisol responses to laboratory stressors.Psychosomatic Medicine70(6)661
- 48. Craft LL (2005) Exercise and clinical depression: Examining two psychological mechanisms. Psychology of Sport & Exercise 6(2): 151–171.LL Craft2005Exercise and clinical depression: Examining two psychological mechanisms.Psychology of Sport & Exercise6(2)151171
- 49. American College of Sports Medicine Position Stand. (1998) The recommended quantity and quality of exercise for developing and maintaining cardiorespiratory and muscular fitness, and flexibility in healthy adults. Med Sci Sports Exerc 30(6): 975–991.American College of Sports Medicine Position Stand.1998The recommended quantity and quality of exercise for developing and maintaining cardiorespiratory and muscular fitness, and flexibility in healthy adults.Med Sci Sports Exerc30(6)975991
- 50. Castillo-Garzón MJ, Ruiz JR, Ortega FB, Gutiérrez Á (2006) Anti-aging therapy through fitness enhancement. Clin Interv Aging 1(3): 213–220.MJ Castillo-GarzónJR RuizFB OrtegaÁ. Gutiérrez2006Anti-aging therapy through fitness enhancement.Clin Interv Aging1(3)213220
- 51. Dunn AL, Trivedi MH, Kampert JB, Clark CG, Chambliss HO (2005) Exercise treatment for depression: Efficacy and dose response. American Journal of Preventive Medicine 28(1): 1–8.AL DunnMH TrivediJB KampertCG ClarkHO Chambliss2005Exercise treatment for depression: Efficacy and dose response.American Journal of Preventive Medicine28(1)18
- 52. McEwen BS (2003) Mood disorders and allostatic load. Biol. Psychiatry 54(3): 200–207.BS McEwen2003Mood disorders and allostatic load. Biol.Psychiatry54(3)200207
- 53. Cohen S, Kamarck T, Mermelstein R (1983) A global measure of perceived stress. Journal of health and social behavior 24(4): 385–396.S. CohenT. KamarckR. Mermelstein1983A global measure of perceived stress.Journal of health and social behavior24(4)385396
- 54. Church TS, Cheng YJ, Earnest CP, Barlow CE, Gibbons LW, et al. (2004) Exercise Capacity and Body Composition as Predictors of Mortality Among Men With Diabetes. Diabetes Care 27(1): 83–88.TS ChurchYJ ChengCP EarnestCE BarlowLW Gibbons2004Exercise Capacity and Body Composition as Predictors of Mortality Among Men With Diabetes.Diabetes Care27(1)8388
- 55. Fransson E, Knutsson A, Westerholm P, Alfredsson L (2008) Indications of recall bias found in a retrospective study of physical activity and myocardial infarction. Journal of Clinical Epidemiology 61(8): 840–847.E. FranssonA. KnutssonP. WesterholmL. Alfredsson2008Indications of recall bias found in a retrospective study of physical activity and myocardial infarction.Journal of Clinical Epidemiology61(8)840847
- 56. Verbrugge LM (1980) Health diaries. Medical Care 18(1): 73–95.LM Verbrugge1980Health diaries.Medical Care18(1)7395
- 57. Gillmore MR, Gaylord J, Hartway J, Hoppe MJ, Morrison DM (2001) Daily Data Collection of Sexual and Other Health-Related Behaviors. The Journal of Sex Research 38(1): 35–42.MR GillmoreJ. GaylordJ. HartwayMJ HoppeDM Morrison2001Daily Data Collection of Sexual and Other Health-Related Behaviors.The Journal of Sex Research38(1)3542
- 58. Physical Activity for Everyone: Guidelines: Adults | DNPAO | CDC [Internet] (2009) [Internet] Physical Activity for Everyone: Guidelines: Adults | DNPAO | CDC2009Available from: http://www.cdc.gov/physicalactivity/everyone/guidelines/adults.html. Available from: http://www.cdc.gov/physicalactivity/everyone/guidelines/adults.html.
- 59. Nelson ME, Rejeski WJ, Blair SN, Duncan PW, Judge JO (2007) Physical activity and public health in older adults. Recommendation from the American College of Sports Medicine and the American Heart Association. Circulation 116(9): 1094–1105.ME NelsonWJ RejeskiSN BlairPW DuncanJO Judge2007Physical activity and public health in older adults. Recommendation from the American College of Sports Medicine and the American Heart Association.Circulation116(9)10941105
- 60. Cawthon RM (2002) Telomere measurement by quantitative PCR. Nucleic acids research 2002; 30(10): e47.RM Cawthon2002Telomere measurement by quantitative PCR.Nucleic acids research 2002;30(10)e47
- 61. Lin J, Epel E, Cheon J, Kroenke C, Sinclair E, et al. (2010) Analysis and comparisons of telomerase activity and telomere length in human T and B cells: Insights for epidemiology of telomere maintenance. Journal of Immunological Methods 352(1–2): 71–80.J. LinE. EpelJ. CheonC. KroenkeE. Sinclair2010Analysis and comparisons of telomerase activity and telomere length in human T and B cells: Insights for epidemiology of telomere maintenance.Journal of Immunological Methods352(1–2)7180