Titania dioxide (TiO2) photocatalyst is primarily induced by ultraviolet light irradiation. Visible-light responsive anion-doped TiO2 photocatalysts contain higher quantum efficiency under sunlight and can be used safely in indoor settings without exposing to biohazardous ultraviolet light. The antibacterial efficiency, however, remains to be further improved.
Using thermal reduction method, here we synthesized silver-nanostructures coated TiO2 thin films that contain a high visible-light responsive antibacterial property. Among our tested titania substrates including TiO2, carbon-doped TiO2 [TiO2 (C)] and nitrogen-doped TiO2 [TiO2 (N)], TiO2 (N) showed the best performance after silver coating. The synergistic antibacterial effect results approximately 5 log reductions of surviving bacteria of Escherichia coli, Streptococcus pyogenes, Staphylococcus aureus and Acinetobacter baumannii. Scanning electron microscope analysis indicated that crystalline silver formed unique wire-like nanostructures on TiO2 (N) substrates, while formed relatively straight and thicker rod-shaped precipitates on the other two titania materials.
Our results suggested that proper forms of silver on various titania materials could further influence the bactericidal property.
Citation: Wong M-S, Sun D-S, Chang H-H (2010) Bactericidal Performance of Visible-Light Responsive Titania Photocatalyst with Silver Nanostructures. PLoS ONE 5(4): e10394. doi:10.1371/journal.pone.0010394
Editor: Stefan Bereswill, Charité-Universitätsmedizin Berlin, Germany
Received: March 19, 2010; Accepted: April 6, 2010; Published: April 29, 2010
Copyright: © 2010 Wong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by National Science Council of Taiwan ROC under grant no. 95-2314-B-320-009-MY3, 95-2120-M-259-003 and 96-2221-E-259-010-MY3, and Ministry of Economic Affairs of Taiwan ROC under grant no. 98-EC-17-A-19-S2-0111. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Since the widespread use of antibiotics and the emergence of more resistant and virulent strains of microorganisms, there is an urgent need to develop alternative sterilization technologies. Traditional ultraviolet (UV) light responsive titania dioxide (TiO2) photocatalyst has been demonstrated to eliminate organic compounds and use as disinfectants . The photon energy generates pairs of electron and hole that yield reactive oxygen species (ROS) such as hydroxyl radicals (· OH) and superoxide anions (O2−) that working as biocides . Since TiO2 is a chemically inert compound, which can exert antimicrobial activity persistently and be controlled by illumination; it seems to be a conceptually feasible alternative. Because it absorbs only 2–3% UV-range of solar light impinging on the Earth's surface when used for an outdoor setting , and UV-light can induce damages of human cells and prohibits the use in our living environments, new generation of visible-light responsive titania photocatalysts were developed . To extend the light-absorption into visible-light range thereby increasing quantum efficiency and safety, doping with transition metal ions and/or anions (negative ions) on TiO2 is a commonly used method, by which it creates intra-band gap states close to the conduction or valence band edges that induces visible-light absorption at the sub-band gap energies , . The ion/anion doped materials may also inhibit the charge recombination, thereby increase the photocatalytic activity , –. Using such approach, several studies have shown to develop titania photocatalyst with antimicrobial activity in the visible-light range –. The drawback of visible-light titania photocatalyst, however, is the low antibacterial efficiency as compared with traditional chemical disinfectants.
Among the doping materials for the development of visible-light titania photocatalyst, silver is one of the interesting element. Silver has been used as bactericidal agent in hygiene and medicinal applications for thousands of years . Currently the antibacterial property of silver is wildly used for managing and cleaning of burn, trauma and diabetic wounds, catheter, and dental silver amalgams , . Both ions (Ag+) and nanoparticles of silver were shown to have antibacterial activity , , . Silver ions could affect the bacterial membrane respiratory electron transport chains and DNA replication components . Studies indicated that silver/TiO2 composites may enhance the photocatalytic destruction of E. coli –; however, the bacterial-killing enhancement of photocatalysis might not apply to all forms of silver coating . These results indicated that silver/TiO2 composted materials may contain the advantages of both materials: silver has a higher antimicrobial activity, and TiO2 can last longer, and able to be controlled by illumination. The conditions of silver-coating, however, might need a defined justification. Thus, here we investigated the antibacterial performance of silver containing visible-light responsive TiO2 (C) and TiO2 (N) substrates, and the influence of silver dosage/deposition status on the bactericidal property using bacterium E. coli. The antibacterial activity against pathogenic bacteria Streptococcus pyogenes, Staphylococcus aureus, and Acinetobacter baumannii was also discussed.
Bactericidal activities of TiO2 substrates coated with or without silver
To investigate the bactericidal property of TiO2 substrate with or without silver, the survival rate of E. coli (1×107 CFU) after visible-light-induced photocatalysis on four substrates including Si, TiO2, Ag/Si, and Ag/TiO2 were examined. We found that silver-coating alone is sufficient to exhibit a strong antibacterial activity without illumination (Fig. 1 “Dark” condition in both Ag/Si, and Ag/TiO2 groups). In addition, visible light illumination on Ag/TiO2 substrates further enhanced the bactericidal property (Fig. 1 “Light” vs. “Dark” condition in Ag/TiO2 groups). Notably, TiO2 is primarily working as an UV-light responsive photocatalyst. These results suggested that silver-depositions in this system changed the light responsiveness and the antibacterial property of TiO2 surfaces.
Antibacterial activities against E. coli of Ag/TiO2-coated silicon substratum with (Light) or without (Dark) visible light illumination were shown. ** P<0.01 as compared with both “Dark” and “Light” groups on Si and TiO2 substrates, and “Dark” groups on Ag/TiO2 substrates.
Different silver-depositions on carbon- and nitrogen-doped TiO2 substrates
Previously we found that dopants of carbon and nitrogen could enhance the visible-light induced antimicrobial property of TiO2 , , , here we would like to further investigate the influence of silver-depositions on carbon- and nitrogen-doped TiO2 substrates [TiO2 (C) and TiO2 (N)]. In addition, we also investigated whether we could control the silver deposition through changing the AgNO3 concentration during thermal reduction. As a result, TiO2, TiO2 (C) and TiO2 (N) substrates were coated with silver by thermal reduction method using different AgNO3 concentration. During the preparation of sliver-containing photocatalytic surfaces (300°C, 3 hours), AgNO3 decomposed completely and transform totally into pure silver. Thus, the average amount of silver depositions on the three different samples [TiO2, TiO2 (C) and TiO2 (N)] is the equal in groups with same AgNO3 concentration (data not shown). E. coli (1×107 CFU) were then subjected to visible-light induced catalysis on these substrates. We found that dopants of nitrogen [Ag/TiO2 (N) groups] further enhanced the bactericidal performance of Ag/TiO2 (Fig. 2, ** P<0.01 as compared with respective Ag/TiO2 groups). Furthermore, 0.1 M AgNO3 is the best condition used herein, since either higher (1 M) or lower (0.0125 M) concentration of AgNO3 resulted a less performance [Fig. 2, 0.1 M vs. 1 M and 0.0125 M in Ag/TiO2 (N) groups].
TiO2, TiO2 (C) and TiO2 (N) substrates were coated with silver depositions by thermal reduction method using solutions containing 1 M, 0.1 M and 0.0125 M of AgNO3, respectively. Surviving E. coli (CFU) on respective substrates after photocatalysis (1.2×103 lux, 10 min) was shown. ** P<0.01 as compared with respective Ag/TiO2 and Ag/TiO2 (C) groups.
To obtain a kinetic data, we further analyzed the E. coli on Ag/TiO2 (N) substrates (0.1 M AgNO3 groups in Fig. 2) that illuminated by visible light at various time points (Fig. 3). It showed that Ag/TiO2 (N) substrates could efficiently reduce the E. coli population in dark, and the exposure to various degrees of illumination could further enhance the antibacterial performance over time (Fig. 3).
Antibacterial activity of Ag/TiO2 (N) substrates was evaluated using E. coli. Visible-light illumination (Light) was carried out at a light density of 1.2×103 lux (30 mW/cm2) for different time points. The silver coating is derived from thermal reduction of 0.1 M AgNO3. After photocatalytic treatments, the surviving bacteria (CFU) were then compared with groups without light illumination (Dark). ** P<0.01, as compared with both 0 min groups and respective groups without illumination (Dark).
Electron microscopic analysis
We used scanning electron microscope (SEM) to investigate whether there are morphological differences of Ag-depositions on TiO2, TiO2 (C) and TiO2 (N) substrates (0.1 M AgNO3 groups in Fig. 2). The SEM images indicated that the amount of crystalline silver on TiO2 (N) surfaces was not significantly higher then those on TiO2 and TiO2 (C) groups (Fig. 4, E vs. A, C and F vs. B, D). Thus, the better performance of Ag/TiO2 (N) is not attributed to higher amount of bactericidal silver-coating. Intriguingly, besides micrometer-scale crystals with rectangular parallelepiped shapes, all three groups showed deposition of fibrous silver nanoparticles on their surfaces (Fig. 4A–F). In addition, we found unique wire-like crystalline silver on TiO2 (N) groups (Fig. 4, E, F vs. A–D; arrow heads in F). When compared with fibrous rod-shaped crystalline silver on Ag/TiO2 and Ag/TiO2 (C) groups with frequently observed crossings (Fig. 4B, D, arrows), these wire-like crystalline silver on TiO2 (N) surfaces seem to be adhered relatively closer to TiO2 surfaces without obvious crossing fibers (Fig. 4E, F).
TiO2 (A, B), TiO2 (C) (C, D) and TiO2 (N) (E, F) substrates were coated with silver (thermal reduced from 0.1 M AgNO3). Images with both low (A, C, E) and high (B, D, F) magnifications were shown. Arrows indicate the crossings of the rod-shaped silver nanoparticles (B, D); arrow heads indicate the wire-like silver nanoparticles (F). Scale bar: 10 µm (A, C, E), 5 µm (B, D, F).
Bactericidal activities of Ag/TiO2 on the elimination of pathogens
To further investigate the performance of Ag/TiO2 (N) to eradicate pathogenic bacteria, human pathogens including S. pyogenes, S. aureus and A. baumannii were subjected to visible-light induced catalysis on Ag/TiO2 (N) substrates (0.1 M AgNO3 groups). We found that all tested pathogens were significantly eliminated. The effectiveness showed an approximately 5 log reduction of bacterial population, which was not influenced by whether the target was Gram-positive or Gram-negative bacteria (Fig. 5).
Photocatalysis-mediated antibacterial activity of Ag/TiO2 (N) substrates by visible-light illumination was evaluated using nonpathogenic E. coli, and pathogenic bacteria including S. pyogenes, S. aureus, and A. baumannii. The silver coating on TiO2 (N) substrates was thermal reduced from 0.1 M AgNO3. For each pathogen, the viable bacterial number of initial inputs (untreated groups, 1×107 CFU) was normalized to 100%. ** P<0.01 compared to the respective untreated groups.
Antimicrobial property of silver has been used to treat burns and wounds for thousands of year . Compared to silver, photocatalyst-based disinfectants are still under developmental stage. Although the bactericidal activity is relatively less than silver products, chemically inert TiO2 materials have the advantages to exert its bactericidal activity persistently and to be modulating by light that makes it potentially to be applied on different usages. Besides the advantage of antibacterial property, however, there are still unavoidable side effects of both silver and TiO2 products. Exposure to soluble silver compounds may elicit toxic effects on respiratory and circulation systems, and organ damages on eye, skin, liver and kidney . Chronic effects are involving permanent bluish-grey discoloration of the skin (argyria) and eyes (argyrosis) . Currently there is no report referring to the toxic effect of TiO2 thin films. TiO2 nanoparticles were shown have no toxic effect after ingested by animals . Ultrafine-TiO2 particles, however, induced pulmonary toxicity in rats . Combined treatments would reduce the affective dosage of both agents and may thus reduce the side effects. Synergistic and enhanced effects were shown in the combined treatments of silver with other antibacterial agents like UV-irradiation and antibiotics –. Studies using silver/TiO2 combination were also indicated. Despite the high antibacterial activity of silver alone that consistently appeared in all relevant literatures, the photocatalytic destruction of E. coli was enhanced in some –, but not all conditions . This indicates that a re-justification of silver coating is needed. As a result, here we choose thin films, a non-toxic form of TiO2 to establish the correlation of thermo-reduction/substrate conditions with antibacterial performance. In addition to commonly used bacterium E. coli, we also tested relatively rarely discussed pathogens. Here we demonstrated the first time that silver and visible-responsive photocatalyst substrates TiO2 (N) synergistically eliminated pathogens under visible-light illumination. Previously we found that illuminating TiO2 (N) substrates could only result in less than one log inhibition of bacterial population , ; while in this study, TiO2 (N) substrates caused two log reduction in companion with silver (Fig. 3 Light vs. Dark in 5 and 10 min groups). Since TiO2 (N)-mediated photocatalysis could injure the bacterial spores and made them more vulnerable to host immune system , such injuries might also lead to a higher sensitivity of bacteria in responding to silver treatments. Detail mechanism of this synergistic effect is worthy to be further investigated.
Here we found the superior antibacterial activity is associated with the formation of wire-like silver nanoparticles. Size and shape were shown to influence the bactericidal property of silver nanoparticles , . Basically, the smaller size they are, the greater their surface area to volume ratio and the higher microbial contacting efficiency. Evidence indicates the silver nanoparticles interacting with human immunodeficiency virus (HIV)-1 in a size dependent manner ; and silver particles smaller than 10 nm have a preferentially higher bactericidal activity as compared to larger particles up to 100 nm . Experiments using silver nanoparticles with different shapes further indicated that truncated triangular nanoparticles got a best antibacterial performance with an affective dose at 0.1 µg/L, as compared with spherical- and rod-shape particles with affective doses at 1.25 µg/L and 5–10 µg/L, respectively . These results suggested that both size and shape are critical factors to determine the antibacterial property of silver nanoparticles. As a result, it is reasonable that wire-shaped silver nanoparticles/TiO2 (N) substrates exhibit a stronger bactericidal activity. Specific role of wire-like structure contribute to the antibacterial effect of silver remains to be further investigated.
Taken together, here we successfully demonstrated that the antibacterial property of silver could be optimized through controlling the thermo-reducing conditions of silver on titania substrates. The silver and TiO2 (N) composites showed a synergistic antibacterial activity under visible light illumination. These findings suggest that silver/TiO2 (N) composited material has potential application in the development of alternative disinfectants.
Materials and Methods
Preparation of Ag-coated TiO2, TiO2 (C) and TiO2 (N) substrates
The substrates used to coat silver nanostructures were three types of titania films, pure TiO2, carbon doped TiO2 (C) and nitrogen-doped TiO2 (N) deposited on Si or quartz coupons using an ion-assisted electron-beam evaporation system (Branchy Vacuum Technology Co., Ltd., Taoyuan, Taiwan) as described . The base pressure of the deposition chamber was below 2.7×10−4 Pa. The substrates were sputter-etched with argon ions (Ar+) for 5 minutes prior to the deposition to remove any residual pollutants on the surface. The substrate temperature was maintained at 300°C by quartz lamps. The TiO2 films were deposited in oxygen atmosphere (6.7×10−3 Pa) using rutile TiO2 (99.99%) as a source material. The doped titania films were prepared in a similar manner as pure titania except the bombardment of energetic dopant ions on the growing film through the ion gun. The nitrogen flow for TiO2−xNx films was 15 standard cm3 min−1 through the ion gun and the chamber pressure was at 4.4×10−2 Pa. The carbon dioxide gas flow for TiO2−xCx films was 7 standard cm3 min−1, and the chamber pressure was 2.6×10−2 Pa. The ion gun beam current of 10 mA and voltage of −1000 V were maintained by a Commonwealth Scientific ion beam power supply controller. The three films were prepared under the optimized conditions for their categories of anatase crystallinity and dopant concentration –.
In the preparation of silver nanostructures by thermal reduction method, the titania film coated substrates were placed in 1 M, 0.1 M and 0.0125 M aqueous solutions of silver nitrate, AgNO3 (Sigma-Aldrich Corp., USA), and then pulled out. Thus, a layer of silver nitrate solution was absorbed on substrate surface. These substrates with silver nitrate were placed in an aluminum oxide crucible and heat-treated in a furnace in air. The heat-treatment temperature was raised at a rate of 5°C per minute to 200°C, maintained for 20 minutes, and then raised again at the same rate to 300°C. They were finally furnace-cooled after being held at 300°C for 3 hours.
Bacterial strains and culture
E. coli (strain OP50) was maintained and cultured Luria-Bertani broth or Luria-Bertani broth agar (MDBio, Inc. Taipei, Taiwan) as previously described , . Streptococcus pyogenes (strain M29588) and S. aureus (strain SA02) (without antibiotic resistance) and pan-drug resistance A. baumannii (PDRAB; strain M36788), are clinical isolates from the Buddhist Tzu-Chi General Hospital, Hualien, Taiwan. S. pyogenes, S. aureus were grown in tryptic soy broth supplemented with 0.5% yeast extract (TSBY) broth or TSBY broth agar (MDBio, Inc. Taipei, Taiwan) , .
Bacterial killing experiments
In this study, bacterial concentrations were either determined by the standard plating method or inferred from optical density readings at 600 nm (OD600). For each bacterium, a factor for converting the OD600 values of the bacterial culture to concentration (CFU/ml) was calculated as the followings. A fresh bacterial culture was diluted by factors of 10−1 to 10−7, and OD600 of these dilutions was measured. Bacterial concentrations of these dilutions were determined using standard plating method. The OD600 values were plotted against the bacterial concentrations' log values, and the conversion factors for particular bacteria were calculated. The conversion factor for S. aureus, for example, was calculated to be 1×108 CFU/ml per OD600 by this method.
In order to determine the bactericidal effects of the silver-coated titania films, 200 µl of bacterial overnight culture was transferred into 5 ml of culture medium and incubated at 37°C until an OD600 of 0.3 to 0.6 (log phase) was reached. The bacterial concentrations were calculated using the conversion factor for the bacteria, and the cultures were diluted to 1×108 CFU/ml with culture medium. One hundred microliters of the bacterial culture (1×107 CFU) was then applied to an area of approximately 2 cm2 of the different substrates using a plastic yellow tip. Before loaded with bacterial solution, the Ag/TiO2 surfaces were gently wished with distilled water for three times. The bacteria substrates were then placed under an incandescent lamp (Classictone incandescent lamp, 60 W, Philips, Taiwan) for photocatalytic reaction, and a light meter (model LX-102, Lutron Electronic Enterprises, Taiwan) was used to record the illumination density. The photocatalytic reaction (bacterial killing) was carried out in a 4°C cold room to eliminate the effect of heat. In the initial experiments, illuminations were carried out for 10 min at a distance of 10 cm, corresponding to the illumination density of 1.2×103 lumen/m2 (lux) (30 mW/cm2). In the kinetic analysis experiments, illuminations were carried out for 1, 5, and 10 min at a distance of 10 cm, and illumination density of 1.2×103 lux (30 mW/cm2). After illumination, the bacterial solutions were recovered from the titania substrates, and an aliquot of fresh culture medium (250 µl) was used to flush the wells through repeatedly pipetting to further collect the residual bacteria on the wells of the culture dish. The two bacterial solutions were pooled to make a total of 350 µl. The bacterial concentration was determined by the standard plating method immediately after the bacterial collection, and percentage of surviving bacteria was calculated.
Scanning electron microscopic imaging
Scanning electron microscopic (SEM) images were obtained using a JEM6500-F field-emission scanning electron microscopy (FESEM) (JEOL Ltd., Tokyo, Japan).
All results were calculated from data of three independent experiments. T-test was used to assess statistical significance of differences in results of the antimicrobial effects. A P value of less than 0.05 (P<0.05) was considered significant. The statistical tests were carried out and output to graphs using the Microsoft Excel (Microsoft Taiwan, Taipei, Taiwan) and SigmaPlot (Systat Software, Point Richmond, CA, USA) software.
The authors wish to thank Mr. Pai-Tsang Huang (Department of Life Science, Tzu-Chi University, Taiwan) and Miss Chung-Hsuan Mu (Department of Materials Science and Engineering, National Dong-Hwa University, Taiwan) for their help on the preparation of photocatalytic materials and the bacterial killing experiments.
Conceived and designed the experiments: MSW DSS HHC. Performed the experiments: MSW HHC. Analyzed the data: MSW DSS HHC. Contributed reagents/materials/analysis tools: MSW. Wrote the paper: HHC.
- 1. Maness PC, Smolinski S, Blake DM, Huang Z, Wolfrum EJ, et al. (1999) Bactericidal activity of photocatalytic TiO(2) reaction: toward an understanding of its killing mechanism. Appl Environ Microbiol 65: 4094–4098.
- 2. Fujishima A, Honda K (1972) Electrochemical photolysis of water at a semiconductor electrode. Nature 238: 37–38.
- 3. Kisch H, Macyk W (2002) Visible-light photocatalysis by modified titania. Chemphyschem 3: 399–400.
- 4. Asahi R, Morikawa T, Ohwaki T, Aoki K, Taga Y (2001) Visible-light photocatalysis in nitrogen-doped titanium oxides. Science 293: 269–271.
- 5. Hashimoto K, Irie H, Fujishima A (2005) TiO2 Photocatalysis: A Historical Overview and Future Prospects. Japanese Journal of Applied Physics 44: 8269–8285.
- 6. Rana S, Rawat J, Sorensson MM, Misra RD (2006) Antimicrobial function of Nd3+-doped anatase titania-coated nickel ferrite composite nanoparticles: a biomaterial system. Acta Biomater 2: 421–432.
- 7. Sunkara BK, Misra RD (2008) Enhanced antibactericidal function of W4+-doped titania-coated nickel ferrite composite nanoparticles: a biomaterial system. Acta Biomater 4: 273–283.
- 8. Li Q, Xie R, Li YW, Mintz EA, Shang JK (2007) Enhanced visible-light-induced photocatalytic disinfection of E. coli by carbon-sensitized nitrogen-doped titanium oxide. Environ Sci Technol 41: 5050–5056.
- 9. Rana S, Rawat J, Misra RD (2005) Anti-microbial active composite nanoparticles with magnetic core and photocatalytic shell: TiO2-NiFe2O4 biomaterial system. Acta Biomater 1: 691–703.
- 10. Rana S, Srivastava RS, Sorensson MM, Misra RD (2005) Synthesis and characterization of nanoparticles with magnetic core and photocatalytic shell: Anatase TiO2–NiFe2O4 system. Materials Science and Engineering B 119: 144–151.
- 11. Rawat J, Rana S, Sorensson MM, Misra RD (2007) Anti-microbial activity of doped anatase titania coated nickel ferrite composite nanoparticles. Materials Science and Technology 23: 97–102.
- 12. Rawat J, Rana S, Srivastava RS, Misra RD (2007) Antimicrobial activity of composite nanoparticles consisting of titania photocatalytic shell and nickel ferrite magnetic core. Materials Science and Engineering C 27: 540–545.
- 13. Wong MS, Chu WC, Sun DS, Huang HS, Chen JH, et al. (2006) Visible-light-induced bactericidal activity of a nitrogen-doped titanium photocatalyst against human pathogens. Appl Environ Microbiol 72: 6111–6116.
- 14. Yu JC, Ho W, Yu J, Yip H, Wong PK, et al. (2005) Efficient visible-light-induced photocatalytic disinfection on sulfur-doped nanocrystalline titania. Environ Sci Technol 39: 1175–1179.
- 15. Mitoraj D, Janczyk A, Strus M, Kisch H, Stochel G, et al. (2007) Visible light inactivation of bacteria and fungi by modified titanium dioxide. Photochem Photobiol Sci 6: 642–648.
- 16. Rengifo-Herrera JA, Mielczarski E, Mielczarski J, Castillo NC, Kiwi J, et al. (2008) Escherichia coli inactivation by N, S co-doped commercial TiO2 powders under UV and visible light. Applied Catalysis B: Environmental 84: 448–456.
- 17. Kau JH, Sun DS, Huang HH, Wong MS, Lin HC, et al. (2009) Role of visible light-activated photocatalyst on the reduction of anthrax spore-induced mortality in mice. PLoS ONE 4: e4167.
- 18. Silver S, Phung le T, Silver G (2006) Silver as biocides in burn and wound dressings and bacterial resistance to silver compounds. J Ind Microbiol Biotechnol 33: 627–634.
- 19. Dunn K, Edwards-Jones V (2004) The role of Acticoat with nanocrystalline silver in the management of burns. Burns 30: Suppl 1S1–9.
- 20. Silver S (2003) Bacterial silver resistance: molecular biology and uses and misuses of silver compounds. FEMS Microbiol Rev 27: 341–353.
- 21. Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 275: 177–182.
- 22. Hu C, Lan Y, Qu J, Hu X, Wang A (2006) Ag/AgBr/TiO2 visible light photocatalyst for destruction of azodyes and bacteria. J Phys Chem B 110: 4066–4072.
- 23. Yao Y, Ohko Y, Sekiguchi Y, Fujishima A, Kubota Y (2008) Self-sterilization using silicone catheters coated with Ag and TiO2 nanocomposite thin film. J Biomed Mater Res B Appl Biomater 85: 453–460.
- 24. Hu C, Guo J, Qu J, Hu X (2007) Photocatalytic degradation of pathogenic bacteria with AgI/TiO2 under visible light irradiation. Langmuir 23: 4982–4987.
- 25. Matsui Y, Otomo K, Ishida S, Yanagihara K, Kawanobe Y, et al. (2004) Effect of silver-carrying photocatalyst “Hikari-Gintech” on mycobacterial growth in vitro. Microbiol Immunol 48: 489–495.
- 26. Choi JY, Kim KH, Choy KC, Oh KT, Kim KN (2007) Photocatalytic antibacterial effect of TiO(2) film formed on Ti and TiAg exposed to Lactobacillus acidophilus. J Biomed Mater Res B Appl Biomater 80: 353–359.
- 27. Cheng CL, Sun DS, Chu WC, Tseng YH, Ho HC, et al. (2009) The effects of the bacterial interaction with visible-light responsive titania photocatalyst on the bactericidal performance. J Biomed Sci 16: 7.
- 28. Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27: 76–83.
- 29. Panyala NR, Pena-Mendez EM, Havel J (2008) Silver or silver nanoparticles: a hazardous threat to the environment and human health? Journal of Applied Biomedicine 6: 117–129.
- 30. Bernard BK, Osheroff MR, Hofmann A, Mennear JH (1990) Toxicology and carcinogenesis studies of dietary titanium dioxide-coated mica in male and female Fischer 344 rats. J Toxicol Environ Health 29: 417–429.
- 31. Warheit DB, Webb TR, Reed KL, Frerichs S, Sayes CM (2007) Pulmonary toxicity study in rats with three forms of ultrafine-TiO2 particles: differential responses related to surface properties. Toxicology 230: 90–104.
- 32. Butkus MA, Labare MP, Starke JA, Moon K, Talbot M (2004) Use of aqueous silver to enhance inactivation of coliphage MS-2 by UV disinfection. Appl Environ Microbiol 70: 2848–2853.
- 33. Shrivastava S, Bera T, Roy A, Singh G, Ramachandrarao P, et al. (2007) Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 18: 225103–225112.
- 34. Shahverdi AR, Fakhimi A, Shahverdi HR, Minaian S (2007) Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. Nanomedicine 3: 168–171.
- 35. Li P, Li J, Wu C, Wu Q, Li J (2005) Synergistic antibacterial effects of β-lactam antibiotic combined with silver nanoparticles. Nanotechnology 16: 1912–1917.
- 36. Iroha IRE, CO, Orji JO, Imomoh OO (2007) Antibacterial efficacy of colloidal silver alone and in combination with other antibiotics on isolates from wound Infections. Scientific Research and Essay 2: 338–341.
- 37. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, et al. (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16: 2346–2353.
- 38. Pal S, Tak YK, Song JM (2007) Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73: 1712–1720.
- 39. Elechiguerra JL, Burt JL, Morones JR, Camacho-Bragado A, Gao X, et al. (2005) Interaction of silver nanoparticles with HIV-1. J Nanobiotechnology 3: 6.
- 40. Wong MS, Hsu SW, Rao KK, Kumar CP (2008) Influence of crystallinity and carbon content on visible light photocatalysis of carbon doped titania thin films. J Molecular Catalysis A: Chemical 279: 20–26.
- 41. Yang MC, Yang TS, Wong MS (2004) Nitrogen-doped titanium oxide films as visible light photocatalyst by vapor deposition. Thin Solid Films 1–5.
- 42. Yang TS, shiu CB, Wong MS (2004) Structure and hydrophilicity of titanium oxide films prepared by electron beam evaporation. Surface Science 548: 75–82.
- 43. Sun DS, Lo SJ, Lin CH, Yu MS, Huang CY, et al. (2005) Calcium oscillation and phosphatidylinositol 3-kinase positively regulate integrin alpha(IIb)beta3-mediated outside-in signaling. J Biomed Sci 12: 321–333.
- 44. Chang JC, Chang HH, Lin CT, Lo SJ (2005) The integrin alpha6beta1 modulation of PI3K and Cdc42 activities induces dynamic filopodium formation in human platelets. J Biomed Sci 12: 881–898.
- 45. Chang CW, Tsai WH, Chuang WJ, Lin YS, Wu JJ, et al. (2007) The fate of SPE B after internalization and its implication in SPEB-induced apoptosis. J Biomed Sci 14: 419–427.