C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) have been shown to be useful for diagnosis of prosthetic hip and knee infection. Little information is available on CRP and ESR in patients undergoing revision or resection of shoulder arthroplasties or spine implants.
We analyzed preoperative CRP and ESR in 636 subjects who underwent knee (n = 297), hip (n = 221) or shoulder (n = 64) arthroplasty, or spine implant (n = 54) removal. A standardized definition of orthopedic implant-associated infection was applied. Receiver operating curve analysis was used to determine ideal cutoff values for differentiating infected from non-infected cases. ESR was significantly different in subjects with aseptic failure infection of knee (median 11 and 53.5 mm/h, respectively, p = <0.0001) and hip (median 11 and 30 mm/h, respectively, p = <0.0001) arthroplasties and spine implants (median 10 and 48.5 mm/h, respectively, p = 0.0033), but not shoulder arthroplasties (median 10 and 9 mm/h, respectively, p = 0.9883). Optimized ESR cutoffs for knee, hip and shoulder arthroplasties and spine implants were 19, 13, 26, and 45 mm/h, respectively. Using these cutoffs, sensitivity and specificity to detect infection were 89 and 74% for knee, 82 and 60% for hip, and 32 and 93% for shoulder arthroplasties, and 57 and 90% for spine implants. CRP was significantly different in subjects with aseptic failure and infection of knee (median 4 and 51 mg/l, respectively, p<0.0001), hip (median 3 and 18 mg/l, respectively, p<0.0001), and shoulder (median 3 and 10 mg/l, respectively, p = 0.01) arthroplasties, and spine implants (median 3 and 20 mg/l, respectively, p = 0.0011). Optimized CRP cutoffs for knee, hip, and shoulder arthroplasties, and spine implants were 14.5, 10.3, 7, and 4.6 mg/l, respectively. Using these cutoffs, sensitivity and specificity to detect infection were 79 and 88% for knee, 74 and 79% for hip, and 63 and 73% for shoulder arthroplasties, and 79 and 68% for spine implants.
CRP and ESR have poor sensitivity for the diagnosis of shoulder implant infection. A CRP of 4.6 mg/l had a sensitivity of 79 and a specificity of 68% to detect infection of spine implants.
Citation: Piper KE, Fernandez-Sampedro M, Steckelberg KE, Mandrekar JN, Karau MJ, Steckelberg JM, et al. (2010) C-Reactive Protein, Erythrocyte Sedimentation Rate and Orthopedic Implant Infection. PLoS ONE 5(2): e9358. doi:10.1371/journal.pone.0009358
Editor: Stefan Bereswill, Charité-Universitätsmedizin Berlin, Germany
Received: January 25, 2010; Accepted: February 3, 2010; Published: February 22, 2010
Copyright: © 2010 Piper et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: There was no external funding source.
Competing interests: The authors have declared that no competing interests exist.
C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR) are inexpensive, non-invasive tests that are often obtained in subjects with orthopedic implants prior to implant removal to assess for implant-associated infection. CRP and, to a lesser extent, ESR, have been shown to be useful in the diagnosis of prosthetic hip and knee infection, especially if validated cut-off values are applied (Table 1). Little information is available, however, on CRP and ESR in patients undergoing revision or resection of shoulder arthroplasties or spine implants.
ESR and CRP have poor sensitivity to detect prosthetic shoulder infection when cutoffs of 30 mm/h or 10 mg/l, respectively, are applied . This may relate to the frequent implication of the low virulence organism, Propionibacterium acnes, in shoulder arthroplasty infection , , or to failure to use optimized cutoff values for shoulder arthroplasty infection. There is little data available on the performance of CRP and ESR in the diagnosis of spine implant-associated infection, although Hahn et al. reported that normal CRP and ESR do not rule out late infection associated with spinal instrumentation .
We analyzed preoperative CRP and ESR in subjects prior to implant removal at our institution, using a standardized definition of orthopedic implant-associated infection, to determine the sensitivity and specificity of CRP and ESR, using receiver operating curve analysis-optimized cutoffs, for the diagnosis of hip, knee, and shoulder arthroplasty and spine implant-associated infection.
Patients who underwent prosthetic knee, hip, or shoulder arthroplasty or spine implant removal between July 2001 and June 2008 at Mayo Clinic Rochester, Minnesota, were studied. CRP (mg/l) and ESR (mm/h) within one month before the index surgery were analyzed. (If more than one measurement was available, the measurement closest to surgery was studied.) Some patients reported herein have been previously described , , . Patients without both CRP and ESR performed within one month before the index surgery were excluded. Patients with underlying inflammatory arthritides were excluded. This study was reviewed and approved by the Mayo Clinic Institutional Review Board. Waivers of informed consent and HIPAA authorization were approved. For subjects residing in Minnesota, only those who had authorized use of their medical record in research (Minnesota Statute 144.335) were included.
Patients were classified as having orthopedic implant-associated infection if at least one of the following was present: (1) Visible purulence surrounding the implant; (2) acute inflammation on histopathologic examination of permanent tissue sections (as determined by the clinical pathologist); (3) a sinus tract communicating with the implant; or (4) positive periprosthetic tissue culture and positive sonicate fluid culture for the same microorganism . Aseptic failure was defined as implant failure not meeting these criteria.
Venous blood was collected in a 3.5 ml serum separator tube for CRP and in a 2.4 ml sodium citrate (0.6 ml) tube for ESR. Intraoperative tissue samples with the most obvious inflammatory changes were collected for histopathologic evaluation and conventional microbiologic culture. Removed implants were placed in an autoclaved (132°C, 27 psi for 15 minutes) 1 liter polypropylene wide mouth container (Nalgene, Lima, Ohio). Specimens were cultured within six hours.
Peri-Implant Tissue Culture
Peri-implant tissue cultures were performed in the Mayo Clinic Clinical Microbiology Laboratory. Tissue specimens were homogenized in 3 ml brain heart infusion broth for 1 minute. The homogenate was inoculated onto aerobic blood, chocolate, and anaerobic blood agar and into thioglycollate broth (BD Diagnostic Systems, Sparks, MD). Aerobic and anaerobic agar plates were incubated at 35–37°C in 5–7% CO2 aerobically and anaerobically for 2–4 and 7 days, respectively. Thioglycollate broth was incubated anaerobically at 35–37°C for 7 days; turbid broth was subcultured. Microorganisms were identified using routine microbiologic techniques. Peri-implant tissue culture positivity was defined as isolation of the same organism from at least two tissue specimens from the index surgery.
Sonicate Fluid Culture
Sterile Ringer's solution (400 ml) was added to each implant-containing container. The container was vortexed for 30 seconds (Vortex Genie, Scientific Industries Inc., Bohemia, NY), followed by bath sonication (frequency 40±2 kHz, power density 0.22±0.04 watts/cm2) in an Aquasonic Model 750T ultrasound bath (VWR Scientific, Weschester, PA) for 5 minutes, and then vortexed again for 30 seconds.
For subjects studied before December 14, 2005, 0.5 ml of sonicate fluid was directly plated onto aerobic and anaerobic sheep blood agar plates which were incubated at 35–37°C in 5–7% CO2 aerobically and anaerobically for 5 and 7 days, respectively. For subjects studied after December 14, 2005, a concentration step and an extended period of anaerobic incubation were added. Sonicate fluid was placed into conical centrifuge tubes which were centrifuged at 3,150×g for 5 minutes. The supernatant was aspirated (for a one hundred fold concentration) and 0.1 ml of the sediment was plated onto aerobic and anaerobic sheep blood agar plates which were incubated at 35–37°C in 5–7% CO2 aerobically and anaerobically for 2–4 and 14 days, respectively. Microorganisms were enumerated and identified using routine microbiologic techniques. Sonicate fluid cultures were considered positive based on the following criteria. A cutoff value of at least 5 colony forming units (cfu) per plate was applied to the subjects studied before December 14, 2005. A cutoff value of at least 20 cfu per plate was applied to the subjects enrolled after December 14, 2005. The higher cutoff for subjects after December 14, 2005 was used due to the addition of a concentration step to the sonicate fluid culture procedure yielding higher numbers of microorganisms .
CRP measurements were performed using the Roche/Hitachi Modular System. Briefly, the anti-CRP antibodies coupled to latex microparticles react with antigen to form antigen-antibody complexes. After agglutination, the complex formation was measured turbidimetrically. The linear range of detection was 3 to 200 mg/l.
Erythrocyte Sedimentation Rate
The ESR was determined using the Westergren method. The rate of sedimentation of erythrocytes is measured in a 1:5 dilution of 3.2% sodium citrate solution to whole blood. Blood is drawn up in a column and allowed to sit undisturbed for one hour. The sedimentation is read as the millimeter distance from the top of the column to the meniscus of the erythrocyte sediment.
Descriptive summaries were reported as median values (due to the non Gaussian distribution of the data). ESR and CRP levels between the aseptic failure and the infection groups at the various anatomic locations (knees, hips, shoulders, spine) were compared using the Wilcoxon rank sum test. All tests were two sided; a p-value of less than 0.05 was considered as statistically significant. Analysis was performed using Statistical Analysis Software (SAS) version 9.0. Receiver operating characteristic curves for ESR and CRP were established for knee, hip, and shoulder arthroplasty and spine implant patients, with the optimal cut-off values chosen as those in the upper left corner of the curve, representing the point at which the greatest sensitivity and specificity are achieved.
There was no external funding source.
Results and Discussion
636 subjects who underwent knee (n = 297), hip (n = 221) or shoulder (n = 64) arthroplasty, or spine implant (n = 54) removal were analyzed. 487 subjects with knee (n = 215), hip (n = 187), or shoulder (n = 45) arthroplasties and spine implants (n = 40) removed met the preoperative definition of aseptic failure. 149 subjects who underwent removal of knee (n = 82), hip (n = 34), or shoulder (n = 19) arthroplasties or spine implants (n = 14) met the preoperative definition of orthopedic implant-associated infection.
CRP was significantly different in subjects with aseptic failure and infection of knee (median 4 and 51 mg/l, respectively, p<0.0001), hip (median 3 and 18 mg/l, respectively, p<0.0001), and shoulder (median 3 and 10 mg/l, respectively, p = 0.01) arthroplasties, and spine implants (median 3 and 20 mg/l, respectively, p = 0.0011). ESR was significantly different in subjects with aseptic failure and infection of knee (median 11 and 53.5 mm/h, respectively, p<0.0001) and hip (median 11 and 30 mm/h, respectively, p<0.0001) arthroplasties, and spine implants (median 10 and 48.5 mm/h, respectively, p = 0.0033), but not shoulder arthroplasties (median 10 and 9 mm/h, respectively, p = 0.9883) (Table 2).
The sensitivities, specificities, positive and negative predictive values, and the p-value from logistic regression of CRP >10 mg/l, ESR >30 mm/h, and CRP >10 mg/l or ESR >30 mm/h to detect infection of knee, hip, and shoulder arthroplasties, and spinal implants are shown in Table 3. The combination of normal ESR (≤30 mm/h) and CRP (≤10 mg/l) predicted the absence of infection in 94, 94 and 91% of subjects undergoing knee or hip arthroplasty or spine implant removal, respectively, but only 77% of those subjects undergoing shoulder arthroplasty removal.
Optimized ESR cutoffs for knee, hip and shoulder arthroplasties and spine implants were 19, 13, 26, and 45 mm/h, respectively. Using these cutoffs, sensitivity and specificity to detect infection were 89 and 74% for knee, 82 and 60% for hip, and 32 and 93% for shoulder arthroplasties, and 57 and 90% for spine implants (Table 4).
Optimized CRP cutoffs for knee, hip, and shoulder arthroplasties, and spine implants were 14.5, 10.3, 7, and 4.6 mg/l, respectively. Using these cutoffs, sensitivity and specificity to detect infection were for 79 and 88% knee, 74 and 79% for hip, and 63 and 73% for shoulder arthroplasties, and 79 and 68% for spine implants (Table 4).
Our study is a comprehensive analysis of CRP and ESR in subjects undergoing orthopedic implant removal with and without infection. In patients satisfying the definition for orthopedic implant-associated infection, CRP and ESR values were higher in knee arthroplasty and spine implant patients than in hip arthroplasty patients. Previous investigations have reported higherCRP and ESR values in knee than hip arthroplasty patients with infection .
We used receiver operating curve analysis to optimize CRP and ESR cutoffs. The optimized CRP cutoff value for hip arthroplasty infection was similar to the standard cutoff of CRP >10 mg/l often used in clinical practice. Optimized CRP and ESR cutoff values for knee arthroplasty in our study were >14.5 mg/l and >19 mm/h, similar to the ≥13.5 mg/l and ≥22.5 mm/h values derived by Greidenaus et al. using a similar approach .
Overall, CRP and ESR showed the lowest sensitivity for diagnosis of shoulder arthroplasty infection, even applying cutoffs optimized using receiving operating curve analysis. This may relate to the predominance of P. acnes in shoulder arthroplasty infection , .
Several investigators have examined the natural history of post-operative ESR and CRP after uncomplicated arthroplasty. CRP levels change more rapidly than ESR levels, and return to normal more rapidly following primary total knee or hip arthroplasty , . CRP levels usually peak on the second or third day following total hip or knee arthroplasty , , , , thereafter dropping to preoperative levels by the third week in total hip arthroplasty patients and by the end of the second month in total knee arthroplasty patients , , . CRP levels rise to a higher level postoperatively in total knee than hip arthroplasty patients . A similar rise and fall of CRP is noted postoperatively in total hip or knee arthroplasty patients with underlying rheumatoid arthritis . One study demonstrated that, after uncomplicated arthroplasty, ESR peaks on the fifth postoperative day , dropping close to preoperative levels at the end of the third month in total hip arthroplasty patients, and at the end of the ninth month in total knee arthroplasty patients . Other studies suggest, however, that the ESR, although usually normal by six months postoperatively, may be elevated for as long as one year after uncomplicated total hip arthroplasty , .
For patients undergoing uncomplicated spinal surgery, investigators have described ESR peaking earlier than described above, on the fourth day, and normalizing within a two week period in the majority of patients . Additionally, levels in patients undergoing fusion surgery were higher than in those patients undergoing herniated disc removal . In patients with known vertebral osteomyelitis the ESR may be elevated for a prolonged period of time, even in the face of appropriate non-operative treatment . In the referenced series, many patients went on to a successful clinical outcome in spite of the elevated ESR, illustrating the poor specificity of the test when used alone to predict treatment failure . The current study differs in that the ESR was used in a predictive fashion preoperatively, in conjunction with the CRP, and a consistent definition of infection was applied.
In a series of 202 revision total hip arthroplasties, all subjects with infection had an elevated ESR (>30 mm/h) or CRP (>10 mg/l) . In our study, this was not the case.
There are several limitations of our study. ESR and CRP are nonspecific markers of inflammation and may be elevated by chronic inflammatory conditions (e.g., rheumatoid arthritis), surgical intervention, or systemic illness. Patients with underlying inflammatory arthritides were excluded from our study, but we did not assess for recent surgeries or systemic illnesses not involving the joint. We assessed ESR and CRP within the month prior to surgery, at a time when the study subjects had symptoms related to implant failure. Ideally, the timing of ESR and CRP measurement should be standardized (e.g., 24 hours prior to surgery). A final limitation is the lack of a Gold standard definition for prosthetic shoulder and spine implant infection.
In conclusion, CRP and ESR values are higher in knee arthroplasty and spine implant patients than in hip arthroplasty patients with infection, and show the lowest sensitivity for diagnosis of shoulder arthroplasty infection, even applying cutoffs optimized using receiving operating curve analysis.
Presented in part at the Musculoskeletal Infection Society Annual Meeting and 19th Open Scientific Meeting, Aug 7–8, 2009, San Diego, California and at the 29th Annual Interscience Conference on Antimicrobial Agents and Chemotherapy/Infectious Diseases Society of America 46th Annual Meeting, October 25–28, 2008.
The authors gratefully acknowledge Andrej Trampuz and Paloma Anguita-Alonso for assistance with data collection.
Conceived and designed the experiments: KEP MFS MJK JMS RP. Performed the experiments: KEP MFS KES JNM MJK JMS RP. Analyzed the data: KEP MFS KES JNM MJK JMS EFB DRO ADH RHC JWS JSS PMH MBD MJY BC RP. Contributed reagents/materials/analysis tools: EFB DRO ADH DGL RHC JWS JSS PMH MBD MJY BC. Wrote the paper: KEP MFS KES JNM MJK JMS EFB DRO ADH DGL RHC JWS JSS PMH MBD MJY BC RP.
- 1. Piper KE, Jacobson MJ, Cofield RH, Sperling JW, Sanchez-Sotelo J, et al. (2009) Microbiologic diagnosis of prosthetic shoulder infection by use of implant sonication. J Clin Microbiol 47: 1878–1884.
- 2. Kanafani ZA, Sexton DJ, Pien BC, Varkey J, Basmania C, et al. (2009) Postoperative joint infections due to Propionibacterium species: A case-control study. Clin Infect Dis 49: 1083–1085.
- 3. Hahn F, Zbinden R, Min K (2005) Late implant infections caused by Propionibacterium acnes in scoliosis surgery. Eur Spine J 14: 783–788.
- 4. Trampuz A, Piper KE, Jacobson MJ, Hanssen AD, Unni KK, et al. (2007) Sonication of removed hip and knee prostheses for diagnosis of infection. N Engl J Med 357: 654–663.
- 5. Fernandez Sampedro M, Huddleston PM, Piper KE, Karua MJ, Dekutoski MB, et al. A biofilm approach to detect bacteria on removed spinal implants. Spine. (In Press).
- 6. Del Pozo JL, Patel R (2009) Infection associated with prosthetic joints. New England Journal of Medicine 361: 43–50.
- 7. Bilgen O, Atici T, Durak K, Karaeminogullari , Bilgen MS (2001) C-reactive protein values and erythrocyte sedimentation rates after total hip and total knee arthroplasty. J Int Med Res 29: 7–12.
- 8. Greidanus NV, Masri BA, Garbuz DS, Wilson SD, McAlinden MG, et al. (2007) Use of erythrocyte sedimentation rate and C-reactive protein level to diagnose infection before revision total knee arthroplasty. A prospective evaluation. J Bone Joint Surg Am 89: 1409–1416.
- 9. Moreschini O, Greggi G, Giordano MC, Nocente M, Margheritini F (2001) Postoperative physiopathological analysis of inflammatory parameters in patients undergoing hip or knee arthroplasty. Int J Tissue React 23: 151–154.
- 10. Niskanen RO, Korkala O, Pammo H (1996) Serum C-reactive protein levels after total hip and knee arthroplasty. J Bone Joint Surg Br 78: 431–433.
- 11. White J, Kelly M, Dunsmuir R (1998) C-reactive protein level after total hip and total knee replacement. J Bone Joint Surg Br 80: 909–911.
- 12. Choudhry RR, Rice RP, Triffitt PD, Harper WM, Gregg PJ (1992) Plasma viscosity and C-reactive protein after total hip and knee arthroplasty. J Bone Joint Surg Br 74: 523–524.
- 13. Larsson S, Thelander U, Friberg S (1992) C-reactive protein (CRP) levels after elective orthopedic surgery. Clin Orthop Relat Res 237–242.
- 14. Shih LY, Wu JJ, Yang DJ (1987) Erythrocyte sedimentation rate and C-reactive protein values in patients with total hip arthroplasty. Clin Orthop Relat Res 238–246.
- 15. Aalto K, Osterman K, Peltola H, Rasanen J (1984) Changes in erythrocyte sedimentation rate and C-reactive protein after total hip arthroplasty. Clin Orthop Relat Res 118–120.
- 16. Laiho K, Maenpaa H, Kautiainen H, Kauppi M, Kaarela K, et al. (2001) Rise in serum C reactive protein after hip and knee arthroplasties in patients with rheumatoid arthritis. Ann Rheum Dis 60: 275–277.
- 17. Jonsson B, Soderholm R, Stromqvist B (1991) Erythrocyte sedimentation rate after lumbar spine surgery. Spine (Phila Pa 1976) 16: 1049–1050.
- 18. Kowalski TJ, Layton KF, Berbari EF, Steckelberg JM, Huddleston PM, et al. (2007) Follow-up MR imaging in patients with pyogenic spine infections: lack of correlation with clinical features. AJNR Am J Neuroradiol 28: 693–699.
- 19. Spangehl M, Masri B, O'Connell J, Duncan C (1999) Prospective analysis of preoperative and intraoperative investigations for the diagnosis of infection at the sites of 202 revision total hip arthroplasties. Journal of Bone and Joint Surgery (American) 81: 672–683.
- 20. Austin MS, Ghanem E, Joshi A, Lindsay A, Parvizi J (2008) A simple, cost-effective screening protocol to rule out periprosthetic infection. J Arthroplasty 23: 65–68.
- 21. Schinsky MF, Della Valle CJ, Sporer SM, Paprosky WG (2008) Perioperative testing for joint infection in patients undergoing revision total hip arthroplasty. J Bone Joint Surg Am 90: 1869–1875.
- 22. Baré J, MacDonald SJ, Bourne RB (2006) Preoperative evaluations in revision total knee arthroplasty. Clin Orthop Relat Res 446: 40–44.
- 23. Bernard L, Lubbeke A, Stern R, Bru JP, Feron JM, et al. (2004) Value of preoperative investigations in diagnosing prosthetic joint infection: retrospective cohort study and literature review. Scand J Infect Dis 36: 410–416.
- 24. Levitsky KA, Hozack WJ, Balderston RA, Rothman RH, Gluckman SJ, et al. (1991) Evaluation of the painful prosthetic joint. Relative value of bone scan, sedimentation rate, and joint aspiration. J Arthroplasty 6: 237–244.
- 25. Feldman DS, Lonner JH, Desai P, Zuckerman JD (1995) The role of intraoperative frozen sections in revision total joint arthroplasty. J Bone Joint Surg Am 77: 1807–1813.
- 26. Bottner F, Wegner A, Winkelmann W, Becker K, Erren M, et al. (2007) Interleukin-6, procalcitonin and TNF-alpha: markers of peri-prosthetic infection following total joint replacement. J Bone Joint Surg Br 89: 94–99.
- 27. Fink B, Makowiak C, Fuerst M, Berger I, Schafer P, et al. (2008) The value of synovial biopsy, joint aspiration and C-reactive protein in the diagnosis of late peri-prosthetic infection of total knee replacements. J Bone Joint Surg Br 90: 874–878.
- 28. Müller M, Morawietz L, Hasart O, Strube P, Perka C, et al. (2008) Diagnosis of periprosthetic infection following total hip arthroplasty - evaluation of the diagnostic values of pre- and intraoperative parameters and the associated strategy to preoperatively select patients with a high probability of joint infection. J Orthop Surg 3: 31.
- 29. Virolainen P, Lahteenmaki H, Hiltunen A, Sipola E, Meurman O, et al. (2002) The reliability of diagnosis of infection during revision arthroplasties. Scand J Surg 91: 178–181.