In eukaryotic cell types, virtually all cellular processes are under control of proline-directed kinases and especially MAP kinases. Serine/threonine kinases in general were originally considered as a eukaryote-specific enzyme family. However, recent studies have revealed that orthologues of eukaryotic serine/threonine kinases exist in bacteria. Moreover, various pathogenic species, such as Yersinia and Mycobacterium, require serine/threonine kinases for successful invasion of human host cells. The substrates targeted by bacterial serine/threonine kinases have remained largely unknown. Here we report that the serine/threonine kinase PknB from the important pathogen Staphylococcus aureus is released into the external milieu, which opens up the possibility that PknB does not only phosphorylate bacterial proteins but also proteins of the human host. To identify possible human targets of purified PknB, we studied in vitro phosphorylation of peptide microarrays and detected 68 possible human targets for phosphorylation. These results show that PknB is a proline-directed kinase with MAP kinase-like enzymatic activity. As the potential cellular targets for PknB are involved in apoptosis, immune responses, transport, and metabolism, PknB secretion may help the bacterium to evade intracellular killing and facilitate its growth. In apparent agreement with this notion, phosphorylation of the host-cell response coordinating transcription factor ATF-2 by PknB was confirmed by mass spectrometry. Taken together, our results identify PknB as the first prokaryotic representative of the proline-directed kinase/MAP kinase family of enzymes.
Citation: Miller M, Donat S, Rakette S, Stehle T, Kouwen TRHM, Diks SH, et al. (2010) Staphylococcal PknB as the First Prokaryotic Representative of the Proline-Directed Kinases. PLoS ONE 5(2): e9057. https://doi.org/10.1371/journal.pone.0009057
Editor: Frank R. DeLeo, National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States of America
Received: November 12, 2009; Accepted: January 18, 2010; Published: February 4, 2010
Copyright: © 2010 Miller et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: Funding for this work was received from the EU (CEU projects LSHM-CT-2006-019064 and LSHG-CT-2006-037469), the transnational SysMO initiative through project BACELL SysMO, the European Science Foundation under the EUROCORES Programme EuroSCOPE, grant 04-EScope 01-011 from the Research Council for Earth and Life Sciences of the Netherlands Organization for Scientific Research, the Top Institute Pharma project T4-213, and the Deutsche Forschungsgemeinschaft (TR34). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Despite their clinical relevance, the mechanisms employed by pathogenic bacteria to subvert the host immune system remain only partially characterised. It has become clear, however, that pathogens create a beneficial environment for their survival by secreting proteins that mimic the functions of several host proteins. One of the best known bacterial examples is Yersinia pestis, the plague bacterium, which injects its effector proteins into the host cells by the type III secretion system , . These Yersinia effector (Yop) proteins include the eukaryotic-like serine/threonine kinase YpkA, also known as YopO . This kinase shows a high degree of sequence similarity to mammalian serine/threonine protein kinase domains. YpkA is translocated into a host cell where it disrupts the actin-based cytoskeletal system and promotes both survival and replication of bacteria by an unknown mechanism , , . Nevertheless, the full spectrum of human proteins that are phosphorylated by YpkA has remained elusive so far .
Eukaryotic-like serine/threonine protein kinases (STPKs) are present not only in the Yersinia genus, but they have also been identified in the soil microorganism Myxococcus xanthus ,  and in human pathogens, such as Mycobacterium tuberculosis, which even encodes 11 STPKs. Only two of these (PknG and PknK) are soluble proteins, while the other nine STPKs contain a transmembrane domain . Moreover STPKs have also been identified in Pseudomonas aeruginosa , , Streptococcus pneumoniae , ,  and in Staphylococcus aureus , , . However, the precise biological functions and substrate specificities of these kinases have not yet been defined.
Recently, attention has been focussed on the PknB kinase of S. aureus. This Gram-positive bacterium is part of the human microbiota, but it can turn into a dangerous pathogen, causing a wide range of infections , , . Although S. aureus is mostly considered as an extracellular pathogen, it can invade a variety of mammalian non-professional cells, such as nasal endothelial cells. Moreover S. aureus survives phagocytosis by professional phagocytes , , , such as neutrophils , , mouse or rat macrophages , , , , and human macrophages , . To overcome the stressful conditions imposed by its host, S. aureus has evolved various protective and offensive responses , , , , such as sensing of environmental stimuli and the activation and inactivation of response regulators , . This is generally achieved through cascades of phosphorylation reactions in the host, which focuses a strong interest on the role of kinases, such as the serine/threonine kinase PknB (also known as StpK) in staphylococcal persistence.
The PknB kinase is composed of three extracellular PASTA domains (penicillin binding domains), a central transmembrane domain and an intracellular kinase domain , . Interestingly, it was recently reported that PknB is not only involved in regulation of the central metabolism of S. aureus , but also determines staphylococcal infection of mouse kidneys in an abscess model . The latter observation raises the question whether the kinase activity of PknB is directly or indirectly involved in the pathogenicity of S. aureus. A direct role of PknB in infection is conceivable since serine/threonine kinases play key roles in mammalian cell signalling, and at least two bacterial equivalents, YpkA of Yersinia and PknG of Mycobacterium tuberculosis, have been shown to be directly involved in the subversion of host cells during the respective infectious processes , . However the exact role played by PknB in pathogenesis or staphylococcal persistence has thus far remained unclear.
Here we show that full-size soluble PknB is present in the medium of growing S. aureus cells. We therefore investigated whether PknB of S. aureus can recognize and phosphorylate known substrates of human serine/threonine kinases. For this purpose, we used peptide microarrays with known human phosphorylation sites. The phosphorylation profile and mass spectrometry results show that PknB is a proline-directed kinase, which can indeed phosphorylate specific human targets. The observed target specificity of PknB indicates possible roles for this enzyme in a wide range of host cell signalling processes during S. aureus infection.
Results and Discussion
Identification of Extracellular PknB
It has previously been reported that different bacteria such as M. tuberculosis and Yersinia species can secrete their eukaryotic-like serine/threonine kinases directly into the host. This mechanism allows these bacteria to survive intracellularly , , to disrupt the actin cytoskeleton , or to cause host cell apoptosis . Since these bacterial ser/thr kinases need to be exported in order to impact on host cells, we investigated whether PknB might be detectable in the extracellular milieu of S. aureus. As shown by Western blotting using polyclonal antibodies against PknB, the full-size PknB was detectable both in the cellular and growth medium fractions of cells of S. aureus NCTC 8325 harvested at an OD600 of 2. As expected, PknB was neither detectable in cellular nor growth medium fractions of the ΔpknB mutant. The precise mechanism by which PknB is liberated from the wild-type cells remains to be elucidated. However, there is precedence for the release of membrane proteins, or fragments thereof, into the extracellular milieu of Gram-positive bacteria, such as S. aureus, by as yet unknown mechanisms . One possibility is that these proteins are released by cell lysis, remaining stable in the medium due to an intrinsic resistance to extracytoplasmic proteolysis . The idea that PknB is released by lysis would be consistent with the detection of relatively small amounts of the cytoplasmic marker protein thioredoxin A (TrxA) in the growth medium fractions (Fig. 1). TrxA is a cytoplasmic bacterial protein, which acts as an antioxidant by facilitating the reduction of cysteine disulfides in other cytoplasmic proteins. Since TrxA is normally a cytoplasmic protein, it will only be found in the extracellular milieu when bacterial cells have lysed. Notably, compared to the cellular samples, we detected relatively more extracellular PknB than extracellular TrxA, which might suggest that the release of PknB is the consequence of a specific process rather than cell lysis.
The S. aureus strain NCTC 8325 (wt) or a ΔpknB derivative were propagated at 37°C in TSB and harvested at OD600 2. Crude extracts (ce) and supernatant (sup) fractions were isolated, corrected for OD and separated by NuPAGE electrophoresis (Invitrogen). Two-fold higher amounts of the supernatant fractions were used for PAGE as compared to the crude extracts. Immunoblotting was conducted using specific antibodies against PknB (upper panel) or TrxA (lower panel). The latter served as an indicator for cell lysis. The position of the specific PknB signal is marked with a black arrow. The band at ∼60 kDA corresponds to an unidentified protein, which cross-reacts with the antibodies against PknB. The molecular weight of marker proteins is indicated on the left.
Irrespective of the mechanism by which PknB is released into the extracellular milieu of S. aureus, its release may impact on human host cell functions. This could be the case upon internalization of S. aureus. Although S. aureus is primarily an extracellular pathogen, there is strong evidence that it can be internalized by a wide range of human host cells. For example, S. aureus invades non-professional phagocytes by a mechanism which requires a specific interaction between the bacterial fibronectin-binding protein and the host cell , , . This leads to host signal transduction, activation of tyrosine kinases, cytoskeletal rearrangement and endosome uptake. Bayles and Qazi reported that internalized S. aureus is able to escape from the host endosome, and this fact opens up the possibility of direct interactions of released PknB with proteins of the human host , 
Eukaryotic Phosphorylation Sites Recognized by PknB
Peptide arrays (PepChips) have previously been used successfully to profile the activity of kinases in eukaryotic cell lysates . We therefore employed this array-based technology to investigate whether the staphylococcal kinase PknB has the ability to recognize and phosphorylate human phosphorylation sites. When the PepChips were incubated with purified and active PknB  and [33P-γ] ATP, radioactivity was efficiently incorporated in a particular subset of the peptides on the chip. In contrast, little radioactivity was incorporated when the arrays were incubated with [33P-γ] ATP in the absence of PknB. We identified 68 potential substrates for PknB, of which the biological functions are summarised in Table 1 (for details, see Table S1). Interestingly, 32 of the potential human substrates of PknB are involved in signal transduction and cell communication. The identified peptides include serine/threonine kinases, cell cycle control proteins, and regulators of protein phosphorylation such as adaptor molecules. Our results suggest that any active PknB released from invasive S. aureus cells may target signal transduction mechanisms for host cell subversion. In addition, 13 potential PknB substrates are involved in gene regulation, including transcription factors, transcription regulatory proteins and RNA binding proteins. Three potential substrates play a role in immune responses and recognition, five in transport processes, ten in cell growth and maintenance (cytoskeletal and structural proteins), two in cell metabolism, two in stress responses, and one in apoptosis.
Phosphorylation of the identified potential human PknB targets would result in significant changes in host cell signal transduction. One of the peptides that is best phosphorylated by PknB (64IVADQTPTPTR74) is derived from the Activating Transcription Factor-2 (ATF-2) (see Table S2). ATF-2 belongs to the bZIP (Basic Leucine Zipper Domain) DNA-binding protein family and is expressed by almost all human cells (GeneNote, Ensemlb ID: ENSG00000115966). In unstimulated cells, ATF-2 is maintained in an inactive form by interactions between its own activation domain and its bZIP domain . In response to certain stimuli , , , the kinases p38 and JNK phosphorylate ATF-2 at amino acids Thr69 and Thr71 , . The phosphorylated ATF-2 can then form homodimers and heterodimers , which bind with high affinity to the consensus sequence 5′-TGACGTCA-3′  in target gene promoters, resulting in their activation. The phosphorylation of ATF-2 thus results in the expression of a broad spectrum of proteins implicated in different processes, such as cell cycle molecules (cyclin D1) , cell adhesion molecules , growth factors , anti-apoptotic factors , and invasion-related molecules .
To confirm that PknB is able to phosphorylate ATF-2, we incubated ATF-2 with PknB in vitro and performed mass spectrometric analyses. As a positive control we incubated ATF-2 with p38. The results show that PknB can indeed phosphorylate ATF-2 (Fig. 2). However, PknB phosphorylated Thr73, whereas p38 phosphorylated Thr69 and Thr71. Interestingly, Thr73 is the known phosphorylation site of the Human Vaccinia-related Kinase 1 (VRK1) . VRK1 is a ser/thr kinase overexpressed in proliferating cells , . Phosphorylation of ATF-2 on Thr 73 by VRK1 kinase leads to the activation of ATF-2 and, consequently, the induction of cellular protection mechanisms.
Recombinant ATF-2 was incubated with PknB (A) or p38 (B) in kinase reaction buffer for 30 minutes at 37°C. As a control, ATF-2 was incubated with PknB in the absence of ATP. After tryptic digestion, the resulting peptides were analyzed via online-mass spectrometry. The panels show the spectra for the ATF-2 peptide VIVADQTPTPTR that was either phosphorylated at the Thr73 by PknB, or Thr 69 and Thr 71 by p38. The b- and y-ions are high-lighted and the observed masses are given. Also the peptide sequence is indicated and amino acids that have been identified my mass spectrometric analysis are indicated in bold letters. The upper sequence corresponds to the b- and the lower sequence to the y-ions.
Another peptide that was very efficiently phosphorylated by PknB belongs to the Bcl-2 interacting protein Bim (see Table S2). In vivo this peptide is recognized by the c-Jun NH2-terminal kinase (JNK) (Table S1) , . Bim is a member of the pro-apoptotic Bcl-2 family of proteins, which play a critical role in apoptosis regulation. A short peptide motif, DKSTQT56P, which is present in BimL and BimEL, but absent from BimS, mediates the binding of Bim to the LC8 cytoplasmic dynein light chain. Importantly, the same motif is also recognized and phosphorylated by PknB (Table S1). Exposure of cells to stress causes the activation of JNK kinase, which phosphorylates Bim at Thr56 in the afore-mentioned DKSTQTP motif. The phosphorylation leads to conformational changes in Bim and subsequent dissociation of Bim from dynein motor complexes . The activated Bim may directly activate pro-apoptotic Bax, or indirectly activate Bax by binding anti-apoptotic Bcl-2 family proteins (e.g., Bcl-2 and Bcl-XL) , . Judged by the observed phosporylation of the DKSTQT56P peptide, the release of PknB from invasive S. aureus cells might have similar effects as its phosphorylation by JNK.
A third intriguing target of PknB identified through phosphorylation profiling is the cytoskeleton-associated protein paxillin. This protein has previously been reported as a target for the phosphatase YopH, which is injected by Yersinia into human host cells, thereby affecting cytoskeleton integrity . Taken together, the identified potential targets of PknB are fully consistent with previously reported effects of S. aureus and other bacteria on host cell apoptosis , , ,  and a wide range of cellular processes , , , , , , .
Interestingly, a comparison of the identified human phosphorylation sites of PknB with staphylococcal proteins revealed about 300 putative PknB target sequences in proteins of S. aureus (data not shown). These include amino acid sequences in proteins that are known to be phosphorylated by PknB, such as triosephosphate isomerase, DnaK, elongation factors, ribosomal proteins and trigger factor .
PknB Is a Proline-Directed Serine/Threonine Kinase
To determine which amino acids are preferably phosphorylated by PknB, we generated a sequence logo based on the 15% best-phosphorylated peptides (Fig. 3). The most frequently found amino acids in PknB-phosphorylated peptides are serine and threonine. This observation is in agreement with the serine/threonine kinase signature in the primary sequence of PknB. Apparently, the signature has portability across the eukaryotic/prokaryotic divides. Nevertheless tyrosine phosphorylation can also be unambiguously identified making this study the first demonstration of enzymatic tyrosine kinase activity in a single isolated prokaryotic enzyme. What is also clearly evident in the sequence logo is the presence of a proline residue next to phosphorylated serine, threonine or tyrosine residues. Thus, it seems that proline is part of the PknB recognition and target sequence. This links PknB to the evolutionary well-conserved family of proline-directed kinases, which includes cyclin-dependent protein kinases (CDKs), mitogen-activated protein kinases (MAP kinases) and glycogen synthase kinase-3 (GSK-3; Fig. 3). These kinases play a crucial role in cell cycle, transcription, signal transduction and are involved in many diseases like cancer or Alzheimer's disease , , . Like the eukaryotic MAP kinases, PknB has the ability to phosphorylate ATF-2 at least in vitro, which implies that PknB has a MAP kinase-like enzymatic activity. This view is supported by the observation that PknB is also involved in the regulation of important cellular functions in S. aureus, including central metabolic pathways  and cell wall metabolism . Taken together, our results imply that PknB is the first prokaryotic representative of the proline-directed kinases, a cardinal family of regulators of eukaryotic cellular physiology. A major challenge for future studies will be to identify human proteins that are phosphorylated by PknB in vivo, for example upon internalization of S. aureus by macrophages.
Materials and Methods
Detection of Extracellular PknB by Immunoblotting
S. aureus NCTC 8325 or a pknB mutant of this strain  were cultivated in 10 ml of TSB (37°C, 250 rpm) and growth was monitored by OD600 readings. Samples of 3 ml were harvested at OD600 2 and cells were separated from the growth medium by centrifugation (8000 rpm, 5 min, 4°C). Bacterial cells were washed in PBS, resuspended in sample buffer (NuPage, Invitrogen) and disrupted using a Precellys 24 bead beater (three times 30 s, 6800 rpm; Bertin Technologies). Proteins from the growth medium fraction were collected by TCA-acetone precipitation . Protein samples were mixed with gel-loading buffer with reducing agent and incubated for 5 min at 95°C. To receive a clear signal for the supernatant fractions twice as much as from the crude extract was applied to gel electrophoresis. The proteins were separated on a 10% Bis-Tris gel (Invitrogen) at 200 V for 35 min in NuPAGE® MES SDS Running Buffer (Invitrogen). The separated proteins were transferred to a nitrocellulose membrane (Protran®, Schleicher and Schuell) by semi-dry blotting at 200 mA for 75 min. Membranes were blocked for one hour in Blocking Buffer (Odyssey, Li-Cor biosciences). Rabbit primary antibodies against the kinase domain of PknB were added (1∶5000 in blocking buffer) and membranes were incubated for 1 hour. Next, membranes were washed 3 times for 5 minutes in PBS-T (Phosphate Buffered Saline Tween-20) before adding a fluorescent secondary antibody at a 1∶20000 dilution in blocking buffer (IRDye 800 CW goat anti-rabbit antibody from LiCor biosciences). Membranes were incubated for 1 hour in the dark, washed three times for 5 min in PBS-T and once in PBS. After transferring the membranes into fresh PBS, they were scanned using the Odyssey Infrared Imaging System (LiCor Biosciences). As a cell lysis control, antibodies against cytoplasmic protein TrxA were used in the same concentration as PknB antibodies.
Cloning, Expression and Purification of S. aureus TrxA
All procedures for DNA purification, restriction, ligation, agarose gel electrophoresis, and transformation of competent E. coli DH5α cells were carried out as previously described . Genomic DNA of S. aureus RN4220  was isolated using the Genelute Bacterial Genomic DNA kit (Sigma). The trxA gene on this genomic DNA was PCR-amplified using the primers GGGGGCATATGGCAATCGTAAAAGTAA and GGGGGCTCGAGTAAATGTTTATCTAAAACTTC. The PCR product was cloned into HincII (HindII) restricted pUC18 . After verification of the sequence, the trxA gene was excised from this plasmid with NdeI and XhoI, and ligated into the same restriction sites of pET26b(+) (Novagen, Inc.), downstream of the T7 promoter and upstream of an in-frame His(6)tag sequence. The resulting pET26-SatrxA plasmid was checked by sequencing and used to transform E. coli BL21(DE3) (Invitrogen) for high-level trxA expression and purification. 10 ml of an overnight culture of this strain was used to inoculate 1 liter fresh LB medium and grown until an OD600 of 0.7 was reached. Then, isopropylthiogalactoside (IPTG) was added to a final concentration of 1 mM to induce TrxA production. After 3 hrs of induction, cells were harvested by centrifugation and resuspended in binding buffer (20 mM NaPi, 300 mM NaCl, 10% (v/v) glycerol, 5 mM imidazole, 3 mM DTT, pH 7.4). Next, cells were disrupted by two passages through a French Press (2500 PSI). Cellular debris was removed by centrifugation (30 min at 30000 g, 4°C), and the clarified supernatant fraction was applied to a nickel-charged IMAC column (5 ml HisTrap HP, GE Healthcare). Unbound sample was washed from the column with binding buffer using an ÄKTA explorer (GE Healthcare). Next, the His-tagged TrxA protein was eluted from the column using binding buffer with a gradient of increasing imidazole concentrations (up to 500 mM imidazole). The eluted fractions were checked for the presence of pure TrxA protein using SDS-PAGE and subsequent silver-staining. Further purification was achieved by concentrating the proteins with Vivaspin columns (Vivascience) and loading them on a Superdex 75 gel filtration column (Amersham) pre-equilibrated with 20 mM NaPi, 150 mM NaCl, 10% glycerol and 3.5 mM DTT, pH 7.4. Fractions containing the purified TrxA proteins were pooled and dialyzed 3 times against 20 mM Tris-HCl, pH 7.6, with 150 mM NaCl. Specific polyclonal antibodies against the purified TrxA protein were raised in rabbits (Eurogentec).
Kinome Array Analysis
PepChip™ Kinomics slides containing 976 fully annotated, disease-related kinase phosphorylation sites in triplicate (Pepscan, Lelystad, The Netherlands, http://www.pepscan.com/) were incubated with 50 µl of the ser/thr kinase PknB incubation mix (0.8 µg/ml PknB kinase domain, 60 mM HEPES, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 50% glycerol, 50 µM ATP supplemented with 1Mbq [γ-33P] ATP, 0.03% Brij-35, 50 µg/ml bovine serum albumin, 3 mM Na3VO4, 50 µg/ml PEG 8000) for 90 min in a humidified incubator. As a negative control we used the “empty” incubation mix without PknB. After incubation the peptide arrays were washed twice in 2 M NaCl (1% Tween-20) and PBS-T. Next, the arrays were rinsed twice in demineralised water and air-dried. The dried slides were transferred to a phosphor imager plate (Fuji Storm 860, Stanford, GE, USA) and exposed for 72 hours. The density of the spots was measured and analyzed with array software.
Peptide Array Data Analysis
To analyze the intensity of spots and to correct for background phosphorylation, the ScanAnalyze software and grid tools were used, and the resulting data were exported to an excel sheet. Three replicate data sets were taken for further statistical analysis. To this end, the Spearman correlation coefficient was calculated for each combination of the three sets. The average and standard deviation for each peptide were determined and plotted in an amplitude-based hierarchical fashion. If only background phosphorylation is present, this amplitude-based distribution can be described by a single exponent. Thus, determining the exponent describing amplitude behavior of the 500 least phosphorylated peptides should give an adequate description of array background phosphorylation and, in practice, this was indeed the case. 125 Peptides which exhibited the incorporation of γ-33P in the absence of added kinase were excluded from further analysis. Peptides of which the average phosphorylation minus 1.96 times the standard deviation was higher as the value expected from describing the background distribution were considered to represent true phosphorylation events. Two-sided heteroscedastic t-tests were also performed on each set of values to determine significance (p<0.05).
Sequence logos were created with the weblogo server at http://weblogo.berkeley.edu/logo.cgi using either the preferred substrates of PknB, or known phosphorylation sequences for human kinases as available at http://www.phosphosite.org/homeAction.do
In Vitro Phosphorylation of ATF-2 by Staphylococcal PknB
In order to confirm the phosphorylation of ATF-2 by PknB, the in vitro assay was performed. Purified staphylococcal kinase PknB (26 µg) was incubated with 50 µg of Activating Transcription Factor fusion protein (Cell Signaling) in kinase incubation mix (50 mM HEPES, 1 mM DTT, 0.01 Brij35, 3 mM MnCl2, 3 mM MgCl2, 50 µM ATP) for 30 minutes at 37°C in waterbath. As a positive control, 0.1 µg of p38-alpha MAP kinase (Cell Signaling) was incubated with 50 µg of ATF-2. As negative controls, the following reaction mixtures were used: PknB with ATF-2 but without ATP; PknB with ATP but without ATF-2; and ATF-2 with ATP but without PknB.
Phosphorylation Site Identification and Protein Identification by Mass Spectrometry
Trypsin (Promega) was activated by 15 min incubation at 30°C in activation buffer and then added 1∶200 to the samples, digestion was allowed to proceed over night at 37°C. The resulting peptides were separated by liquid chromatography and measured online by ESI-mass spectrometry using a nanoACQUITY UPLC™ system (Waters, Milford, MA) coupled to an LTQ Orbitrap™ mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Peptides were desalted onto a trap column (Symmetry® C18, Waters). Elution was performed onto an analytical column (BEH130 C18, Waters) by a binary gradient of buffer A (0.1% (v/v) acetic acid) and B (100% (v/v) acetonitrile, 0.1% (v/v) acetic acid) over a period of 50 min with a flow rate of 400 nl/min. The LTQ Orbitrap was operated in data-dependent MS/MS mode using MSA for phospho-relevant masses. Proteins were identified by searching all MS/MS spectra in .dta format against all S. aureus NCTC 8325 proteins and added ATF-2 protein (extracted from the NCBI database) using Sorcerer™-SEQUEST® (ThermoFinnigan, San Jose, CA; version v.27, rev. 11). Sequest was searched with a fragment ion mass tolerance of 1.00 Da and a parent ion tolerance of 10 ppm. Up to two missed tryptic cleavages were allowed. Methionine oxidation (+15.99492 Da), Carbamidomethylation (+57.021465 Da) and phosphorylation of STY (+79.966331 Da) was set as variable modification. Proteins were identified by at least two peptides applying a stringent SEQUEST filter. Sequest identifications required at least deltaCn scores of greater than 0.10 and XCorr scores of greater than 1.9, 2.2, 3.75 and 3.75 for singly, doubly, triply and quadruply charged peptides. Phosphorylated peptides which passed this filter were examined manually and accepted only, when b- or y- ions confirmed the phosphorylation site.
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The authors wish to thank Michel Débabouillé and Tarek Msadek for useful discussions.
Conceived and designed the experiments: MM AD DB MPP JMvD KO. Performed the experiments: MM SD SR TRHMK SHD AD ER KG. Analyzed the data: MM SD SHD AD DB MPP KO. Contributed reagents/materials/analysis tools: SR TS MPP KO. Wrote the paper: MM SD SR TS TRHMK SHD AD ER KG DB MPP JMvD KO.
- 1. Rosqvist R, Forsberg A, Wolf-Watz H (1991) Microinjection of the Yersinia YopE cytotoxin in mammalian cells induces actin microfilament disruption. Biochem Soc Trans 19: 1131–1132.R. RosqvistA. ForsbergH. Wolf-Watz1991Microinjection of the Yersinia YopE cytotoxin in mammalian cells induces actin microfilament disruption.Biochem Soc Trans1911311132
- 2. Rosqvist R, Magnusson KE, Wolf-Watz H (1994) Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells. EMBO J 13: 964–972.R. RosqvistKE MagnussonH. Wolf-Watz1994Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells.EMBO J13964972
- 3. Galyov EE, Hakansson S, Forsberg A, Wolf-Watz H (1993) A secreted protein kinase of Yersinia pseudotuberculosis is an indispensable virulence determinant. Nature 361: 730–732.EE GalyovS. HakanssonA. ForsbergH. Wolf-Watz1993A secreted protein kinase of Yersinia pseudotuberculosis is an indispensable virulence determinant.Nature361730732
- 4. Juris SJ, Rudolph AE, Huddler D, Orth K, Dixon JE (2000) A distinctive role for the Yersinia protein kinase: actin binding, kinase activation, and cytoskeleton disruption. Proc Natl Acad Sci U S A 97: 9431–9436.SJ JurisAE RudolphD. HuddlerK. OrthJE Dixon2000A distinctive role for the Yersinia protein kinase: actin binding, kinase activation, and cytoskeleton disruption.Proc Natl Acad Sci U S A9794319436
- 5. Wiley DJ, Shrestha N, Yang J, Atis N, Dayton K, et al. (2009) The activities of the Yersinia protein kinase A (YpkA) and outer protein J (YopJ) virulence factors converge on an eIF2alpha kinase. J Biol Chem 284: 24744–24753.DJ WileyN. ShresthaJ. YangN. AtisK. Dayton2009The activities of the Yersinia protein kinase A (YpkA) and outer protein J (YopJ) virulence factors converge on an eIF2alpha kinase.J Biol Chem2842474424753
- 6. Navarro L, Koller A, Nordfelth R, Wolf-Watz H, Taylor S, et al. (2007) Identification of a molecular target for the Yersinia protein kinase A. Mol Cell 26: 465–477.L. NavarroA. KollerR. NordfelthH. Wolf-WatzS. Taylor2007Identification of a molecular target for the Yersinia protein kinase A.Mol Cell26465477
- 7. Munoz-Dorado J, Inouye S, Inouye M (1993) Eukaryotic-like protein serine/threonine kinases in Myxococcus xanthus, a developmental bacterium exhibiting social behavior. J Cell Biochem 51: 29–33.J. Munoz-DoradoS. InouyeM. Inouye1993Eukaryotic-like protein serine/threonine kinases in Myxococcus xanthus, a developmental bacterium exhibiting social behavior.J Cell Biochem512933
- 8. Perez J, Castaneda-Garcia A, Jenke-Kodama H, Muller R, Munoz-Dorado J (2008) Eukaryotic-like protein kinases in the prokaryotes and the myxobacterial kinome. Proc Natl Acad Sci U S A 105: 15950–15955.J. PerezA. Castaneda-GarciaH. Jenke-KodamaR. MullerJ. Munoz-Dorado2008Eukaryotic-like protein kinases in the prokaryotes and the myxobacterial kinome.Proc Natl Acad Sci U S A1051595015955
- 9. Av-Gay Y, Everett M (2000) The eukaryotic-like Ser/Thr protein kinases of Mycobacterium tuberculosis. Trends Microbiol 8: 238–244.Y. Av-GayM. Everett2000The eukaryotic-like Ser/Thr protein kinases of Mycobacterium tuberculosis.Trends Microbiol8238244
- 10. Wang J, Li C, Yang H, Mushegian A, Jin S (1998) A novel serine/threonine protein kinase homologue of Pseudomonas aeruginosa is specifically inducible within the host infection site and is required for full virulence in neutropenic mice. J Bacteriol 180: 6764–6768.J. WangC. LiH. YangA. MushegianS. Jin1998A novel serine/threonine protein kinase homologue of Pseudomonas aeruginosa is specifically inducible within the host infection site and is required for full virulence in neutropenic mice.J Bacteriol18067646768
- 11. Motley ST, Lory S (1999) Functional characterization of a serine/threonine protein kinase of Pseudomonas aeruginosa. Infect Immun 67: 5386–5394.ST MotleyS. Lory1999Functional characterization of a serine/threonine protein kinase of Pseudomonas aeruginosa.Infect Immun6753865394
- 12. Pallova P, Hercik K, Saskova L, Novakova L, Branny P (2007) A eukaryotic-type serine/threonine protein kinase StkP of Streptococcus pneumoniae acts as a dimer in vivo. Biochem Biophys Res Commun 355: 526–530.P. PallovaK. HercikL. SaskovaL. NovakovaP. Branny2007A eukaryotic-type serine/threonine protein kinase StkP of Streptococcus pneumoniae acts as a dimer in vivo.Biochem Biophys Res Commun355526530
- 13. Echenique J, Kadioglu A, Romao S, Andrew PW, Trombe MC (2004) Protein serine/threonine kinase StkP positively controls virulence and competence in Streptococcus pneumoniae. Infect Immun 72: 2434–2437.J. EcheniqueA. KadiogluS. RomaoPW AndrewMC Trombe2004Protein serine/threonine kinase StkP positively controls virulence and competence in Streptococcus pneumoniae.Infect Immun7224342437
- 14. Novakova L, Saskova L, Pallova P, Janecek J, Novotna J, et al. (2005) Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates. FEBS J 272: 1243–1254.L. NovakovaL. SaskovaP. PallovaJ. JanecekJ. Novotna2005Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates.FEBS J27212431254
- 15. Lomas-Lopez R, Paracuellos P, Riberty M, Cozzone AJ, Duclos B (2007) Several enzymes of the central metabolism are phosphorylated in Staphylococcus aureus. FEMS Microbiol Lett 272: 35–42.R. Lomas-LopezP. ParacuellosM. RibertyAJ CozzoneB. Duclos2007Several enzymes of the central metabolism are phosphorylated in Staphylococcus aureus.FEMS Microbiol Lett2723542
- 16. Donat S, Streker K, Schirmeister T, Rakette S, Stehle T, et al. (2009) Transcriptome and functional analysis of the eukaryotic-type serine/threonine kinase PknB in Staphylococcus aureus. J Bacteriol 191: 4056–4069.S. DonatK. StrekerT. SchirmeisterS. RaketteT. Stehle2009Transcriptome and functional analysis of the eukaryotic-type serine/threonine kinase PknB in Staphylococcus aureus.J Bacteriol19140564069
- 17. Debarbouille M, Dramsi S, Dussurget O, Nahori MA, Vaganay E, et al. (2009) Characterization of a serine/threonine kinase involved in virulence of Staphylococcus aureus. J Bacteriol 191: 4070–4081.M. DebarbouilleS. DramsiO. DussurgetMA NahoriE. Vaganay2009Characterization of a serine/threonine kinase involved in virulence of Staphylococcus aureus.J Bacteriol19140704081
- 18. Deftereos SP, Michailidou E, Karagiannakis GK, Grigoriadi S, Prassopoulos P (2009) Hematogenous infantile infection presenting as osteomyelitis and septic arthritis: a case report. Cases J 2: 8293.SP DeftereosE. MichailidouGK KaragiannakisS. GrigoriadiP. Prassopoulos2009Hematogenous infantile infection presenting as osteomyelitis and septic arthritis: a case report.Cases J28293
- 19. Geipel U (2009) Pathogenic organisms in hip joint infections. Int J Med Sci 6: 234–240.U. Geipel2009Pathogenic organisms in hip joint infections.Int J Med Sci6234240
- 20. Carrillo-Marquez MA, Hulten KG, Hammerman W, Mason EO, Kaplan SL (2009) USA300 is the predominant genotype causing Staphylococcus aureus septic arthritis in children. Pediatr Infect Dis J 28: 1076–1080.MA Carrillo-MarquezKG HultenW. HammermanEO MasonSL Kaplan2009USA300 is the predominant genotype causing Staphylococcus aureus septic arthritis in children.Pediatr Infect Dis J2810761080
- 21. Melly MA, Thomison JB, Rogers DE (1960) Fate of staphylococci within human leukocytes. J Exp Med 112: 1121–1130.MA MellyJB ThomisonDE Rogers1960Fate of staphylococci within human leukocytes.J Exp Med11211211130
- 22. Kapral FA (1965) The phagocytosis and intracellular fate of staphylococci. Ann N Y Acad Sci 128: 285–300.FA Kapral1965The phagocytosis and intracellular fate of staphylococci.Ann N Y Acad Sci128285300
- 23. Rogers DE, Tompsett R (1952) The survival of staphylococci within human leukocytes. J Exp Med 95: 209–230.DE RogersR. Tompsett1952The survival of staphylococci within human leukocytes.J Exp Med95209230
- 24. Gresham HD, Lowrance JH, Caver TE, Wilson BS, Cheung AL, et al. (2000) Survival of Staphylococcus aureus inside neutrophils contributes to infection. J Immunol 164: 3713–3722.HD GreshamJH LowranceTE CaverBS WilsonAL Cheung2000Survival of Staphylococcus aureus inside neutrophils contributes to infection.J Immunol16437133722
- 25. Voyich JM, Braughton KR, Sturdevant DE, Whitney AR, Said-Salim B, et al. (2005) Insights into mechanisms used by Staphylococcus aureus to avoid destruction by human neutrophils. J Immunol 175: 3907–3919.JM VoyichKR BraughtonDE SturdevantAR WhitneyB. Said-Salim2005Insights into mechanisms used by Staphylococcus aureus to avoid destruction by human neutrophils.J Immunol17539073919
- 26. Baughn RE, Bonventre (1975) Nonspecific resistance to Listeria monocytogenes in mice infected and elicited with Staphylococcus aureus. Med Microbiol Immunol 161: 243–252.RE BaughnBonventre1975Nonspecific resistance to Listeria monocytogenes in mice infected and elicited with Staphylococcus aureus.Med Microbiol Immunol161243252
- 27. Hebert A, Sayasith K, Senechal S, Dubreuil P, Lagace J (2000) Demonstration of intracellular Staphylococcus aureus in bovine mastitis alveolar cells and macrophages isolated from naturally infected cow milk. FEMS Microbiol Lett 193: 57–62.A. HebertK. SayasithS. SenechalP. DubreuilJ. Lagace2000Demonstration of intracellular Staphylococcus aureus in bovine mastitis alveolar cells and macrophages isolated from naturally infected cow milk.FEMS Microbiol Lett1935762
- 28. Jakab GJ, Green GM (1976) Defect in intracellular killing of Staphylococcus aureus within alveolar macrophages in Sendai virus-infected murine lungs. J Clin Invest 57: 1533–1539.GJ JakabGM Green1976Defect in intracellular killing of Staphylococcus aureus within alveolar macrophages in Sendai virus-infected murine lungs.J Clin Invest5715331539
- 29. Michailova L, Stoitsova S, Markova N, Kussovski V, Jordanova M, et al. (2000) Interaction of alveolar macrophages with Staphylococcus aureus and induction of microbial L-forms during infection in rats. Int J Med Microbiol 290: 259–267.L. MichailovaS. StoitsovaN. MarkovaV. KussovskiM. Jordanova2000Interaction of alveolar macrophages with Staphylococcus aureus and induction of microbial L-forms during infection in rats.Int J Med Microbiol290259267
- 30. Elliott GR, Peterson PK, Verbrugh HA, Freiberg MR, Hoidal JR, et al. (1982) Influence of subinhibitory concentrations of penicillin, cephalothin, and clindamycin on Staphylococcus aureus growth in human phagocytic cells. Antimicrob Agents Chemother 22: 781–784.GR ElliottPK PetersonHA VerbrughMR FreibergJR Hoidal1982Influence of subinhibitory concentrations of penicillin, cephalothin, and clindamycin on Staphylococcus aureus growth in human phagocytic cells.Antimicrob Agents Chemother22781784
- 31. Kubica M, Guzik K, Koziel J, Zarebski M, Richter W, et al. (2008) A potential new pathway for Staphylococcus aureus dissemination: the silent survival of S. aureus phagocytosed by human monocyte-derived macrophages. PLoS ONE 3: e1409.M. KubicaK. GuzikJ. KozielM. ZarebskiW. Richter2008A potential new pathway for Staphylococcus aureus dissemination: the silent survival of S. aureus phagocytosed by human monocyte-derived macrophages.PLoS ONE3e1409
- 32. Foster TJ, Hook M (1998) Surface protein adhesins of Staphylococcus aureus. Trends Microbiol 6: 484–488.TJ FosterM. Hook1998Surface protein adhesins of Staphylococcus aureus.Trends Microbiol6484488
- 33. Lowy FD (1998) Staphylococcus aureus infections. N Engl J Med 339: 520–532.FD Lowy1998Staphylococcus aureus infections.N Engl J Med339520532
- 34. Foster TJ (2005) Immune evasion by staphylococci. Nat Rev Microbiol 3: 948–958.TJ Foster2005Immune evasion by staphylococci.Nat Rev Microbiol3948958
- 35. Sibbald MJ, Ziebandt AK, Engelmann S, Hecker M, de Jong A, et al. (2006) Mapping the pathways to staphylococcal pathogenesis by comparative secretomics. Microbiol Mol Biol Rev 70: 755–788.MJ SibbaldAK ZiebandtS. EngelmannM. HeckerA. de Jong2006Mapping the pathways to staphylococcal pathogenesis by comparative secretomics.Microbiol Mol Biol Rev70755788
- 36. Koziel J, Maciag-Gudowska A, Mikolajczyk T, Bzowska M, Sturdevant DE, et al. (2009) Phagocytosis of Staphylococcus aureus by macrophages exerts cytoprotective effects manifested by the upregulation of antiapoptotic factors. PLoS ONE 4: e5210.J. KozielA. Maciag-GudowskaT. MikolajczykM. BzowskaDE Sturdevant2009Phagocytosis of Staphylococcus aureus by macrophages exerts cytoprotective effects manifested by the upregulation of antiapoptotic factors.PLoS ONE4e5210
- 37. Arbibe L, Mira JP, Teusch N, Kline L, Guha M, et al. (2000) Toll-like receptor 2-mediated NF-kappa B activation requires a Rac1-dependent pathway. Nat Immunol 1: 533–540.L. ArbibeJP MiraN. TeuschL. KlineM. Guha2000Toll-like receptor 2-mediated NF-kappa B activation requires a Rac1-dependent pathway.Nat Immunol1533540
- 38. Treusch AH, Vergin KL, Finlay LA, Donatz MG, Burton RM, et al. (2009) Seasonality and vertical structure of microbial communities in an ocean gyre. ISME J. AH TreuschKL VerginLA FinlayMG DonatzRM Burton2009Seasonality and vertical structure of microbial communities in an ocean gyre.ISME J
- 39. Walburger A, Koul A, Ferrari G, Nguyen L, Prescianotto-Baschong C, et al. (2004) Protein kinase G from pathogenic mycobacteria promotes survival within macrophages. Science 304: 1800–1804.A. WalburgerA. KoulG. FerrariL. NguyenC. Prescianotto-Baschong2004Protein kinase G from pathogenic mycobacteria promotes survival within macrophages.Science30418001804
- 40. Park H, Teja K, O'Shea JJ, Siegel RM (2007) The Yersinia effector protein YpkA induces apoptosis independently of actin depolymerization. J Immunol 178: 6426–6434.H. ParkK. TejaJJ O'SheaRM Siegel2007The Yersinia effector protein YpkA induces apoptosis independently of actin depolymerization.J Immunol17864266434
- 41. Wiley DJ, Nordfeldth R, Rosenzweig J, DaFonseca CJ, Gustin R, et al. (2006) The Ser/Thr kinase activity of the Yersinia protein kinase A (YpkA) is necessary for full virulence in the mouse, mollifying phagocytes, and disrupting the eukaryotic cytoskeleton. Microb Pathog 40: 234–243.DJ WileyR. NordfeldthJ. RosenzweigCJ DaFonsecaR. Gustin2006The Ser/Thr kinase activity of the Yersinia protein kinase A (YpkA) is necessary for full virulence in the mouse, mollifying phagocytes, and disrupting the eukaryotic cytoskeleton.Microb Pathog40234243
- 42. Tjalsma H, Bolhuis A, Jongbloed JD, Bron S, van Dijl JM (2000) Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome. Microbiol Mol Biol Rev 64: 515–547.H. TjalsmaA. BolhuisJD JongbloedS. BronJM van Dijl2000Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome.Microbiol Mol Biol Rev64515547
- 43. Westers L, Westers H, Zanen G, Antelmann H, Hecker M, et al. (2008) Genetic or chemical protease inhibition causes significant changes in the Bacillus subtilis exoproteome. Proteomics 8: 2704–2713.L. WestersH. WestersG. ZanenH. AntelmannM. Hecker2008Genetic or chemical protease inhibition causes significant changes in the Bacillus subtilis exoproteome.Proteomics827042713
- 44. Dziewanowska K, Patti JM, Deobald CF, Bayles KW, Trumble WR, et al. (1999) Fibronectin binding protein and host cell tyrosine kinase are required for internalization of Staphylococcus aureus by epithelial cells. Infect Immun 67: 4673–4678.K. DziewanowskaJM PattiCF DeobaldKW BaylesWR Trumble1999Fibronectin binding protein and host cell tyrosine kinase are required for internalization of Staphylococcus aureus by epithelial cells.Infect Immun6746734678
- 45. Lammers A, Nuijten PJ, Smith HE (1999) The fibronectin binding proteins of Staphylococcus aureus are required for adhesion to and invasion of bovine mammary gland cells. FEMS Microbiol Lett 180: 103–109.A. LammersPJ NuijtenHE Smith1999The fibronectin binding proteins of Staphylococcus aureus are required for adhesion to and invasion of bovine mammary gland cells.FEMS Microbiol Lett180103109
- 46. Ahmed S, Meghji S, Williams RJ, Henderson B, Brock JH, et al. (2001) Staphylococcus aureus fibronectin binding proteins are essential for internalization by osteoblasts but do not account for differences in intracellular levels of bacteria. Infect Immun 69: 2872–2877.S. AhmedS. MeghjiRJ WilliamsB. HendersonJH Brock2001Staphylococcus aureus fibronectin binding proteins are essential for internalization by osteoblasts but do not account for differences in intracellular levels of bacteria.Infect Immun6928722877
- 47. Bayles KW, Wesson CA, Liou LE, Fox LK, Bohach GA, et al. (1998) Intracellular Staphylococcus aureus escapes the endosome and induces apoptosis in epithelial cells. Infect Immun 66: 336–342.KW BaylesCA WessonLE LiouLK FoxGA Bohach1998Intracellular Staphylococcus aureus escapes the endosome and induces apoptosis in epithelial cells.Infect Immun66336342
- 48. Qazi SN, Counil E, Morrissey J, Rees CE, Cockayne A, et al. (2001) agr expression precedes escape of internalized Staphylococcus aureus from the host endosome. Infect Immun 69: 7074–7082.SN QaziE. CounilJ. MorrisseyCE ReesA. Cockayne2001agr expression precedes escape of internalized Staphylococcus aureus from the host endosome.Infect Immun6970747082
- 49. Diks SH, Kok K, O'Toole T, Hommes DW, van Dijken P, et al. (2004) Kinome profiling for studying lipopolysaccharide signal transduction in human peripheral blood mononuclear cells. J Biol Chem 279: 49206–49213.SH DiksK. KokT. O'TooleDW HommesP. van Dijken2004Kinome profiling for studying lipopolysaccharide signal transduction in human peripheral blood mononuclear cells.J Biol Chem2794920649213
- 50. Li XY, Green MR (1996) Intramolecular inhibition of activating transcription factor-2 function by its DNA-binding domain. Genes Dev 10: 517–527.XY LiMR Green1996Intramolecular inhibition of activating transcription factor-2 function by its DNA-binding domain.Genes Dev10517527
- 51. Raingeaud J, Gupta S, Rogers JS, Dickens M, Han J, et al. (1995) Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine. J Biol Chem 270: 7420–7426.J. RaingeaudS. GuptaJS RogersM. DickensJ. Han1995Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine.J Biol Chem27074207426
- 52. Gupta S, Campbell D, Derijard B, Davis RJ (1995) Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science 267: 389–393.S. GuptaD. CampbellB. DerijardRJ Davis1995Transcription factor ATF2 regulation by the JNK signal transduction pathway.Science267389393
- 53. Livingstone C, Patel G, Jones N (1995) ATF-2 contains a phosphorylation-dependent transcriptional activation domain. EMBO J 14: 1785–1797.C. LivingstoneG. PatelN. Jones1995ATF-2 contains a phosphorylation-dependent transcriptional activation domain.EMBO J1417851797
- 54. Vlahopoulos SA, Logotheti S, Mikas D, Giarika A, Gorgoulis V, et al. (2008) The role of ATF-2 in oncogenesis. Bioessays 30: 314–327.SA VlahopoulosS. LogothetiD. MikasA. GiarikaV. Gorgoulis2008The role of ATF-2 in oncogenesis.Bioessays30314327
- 55. Lin YS, Green MR (1988) Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters. Proc Natl Acad Sci U S A 85: 3396–3400.YS LinMR Green1988Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters.Proc Natl Acad Sci U S A8533963400
- 56. Beier F, Taylor AC, LuValle P (2000) Activating transcription factor 2 is necessary for maximal activity and serum induction of the cyclin A promoter in chondrocytes. J Biol Chem 275: 12948–12953.F. BeierAC TaylorP. LuValle2000Activating transcription factor 2 is necessary for maximal activity and serum induction of the cyclin A promoter in chondrocytes.J Biol Chem2751294812953
- 57. Laferriere J, Houle F, Taher MM, Valerie K, Huot J (2001) Transendothelial migration of colon carcinoma cells requires expression of E-selectin by endothelial cells and activation of stress-activated protein kinase-2 (SAPK2/p38) in the tumor cells. J Biol Chem 276: 33762–33772.J. LaferriereF. HouleMM TaherK. ValerieJ. Huot2001Transendothelial migration of colon carcinoma cells requires expression of E-selectin by endothelial cells and activation of stress-activated protein kinase-2 (SAPK2/p38) in the tumor cells.J Biol Chem2763376233772
- 58. Maekawa T, Bernier F, Sato M, Nomura S, Singh M, et al. (1999) Mouse ATF-2 null mutants display features of a severe type of meconium aspiration syndrome. J Biol Chem 274: 17813–17819.T. MaekawaF. BernierM. SatoS. NomuraM. Singh1999Mouse ATF-2 null mutants display features of a severe type of meconium aspiration syndrome.J Biol Chem2741781317819
- 59. Ma Q, Li X, Vale-Cruz D, Brown ML, Beier F, et al. (2007) Activating transcription factor 2 controls Bcl-2 promoter activity in growth plate chondrocytes. J Cell Biochem 101: 477–487.Q. MaX. LiD. Vale-CruzML BrownF. Beier2007Activating transcription factor 2 controls Bcl-2 promoter activity in growth plate chondrocytes.J Cell Biochem101477487
- 60. Song H, Ki SH, Kim SG, Moon A (2006) Activating transcription factor 2 mediates matrix metalloproteinase-2 transcriptional activation induced by p38 in breast epithelial cells. Cancer Res 66: 10487–10496.H. SongSH KiSG KimA. Moon2006Activating transcription factor 2 mediates matrix metalloproteinase-2 transcriptional activation induced by p38 in breast epithelial cells.Cancer Res661048710496
- 61. Sevilla A, Santos CR, Vega FM, Lazo PA (2004) Human vaccinia-related kinase 1 (VRK1) activates the ATF2 transcriptional activity by novel phosphorylation on Thr-73 and Ser-62 and cooperates with JNK. J Biol Chem 279: 27458–27465.A. SevillaCR SantosFM VegaPA Lazo2004Human vaccinia-related kinase 1 (VRK1) activates the ATF2 transcriptional activity by novel phosphorylation on Thr-73 and Ser-62 and cooperates with JNK.J Biol Chem2792745827465
- 62. Nezu J, Oku A, Jones MH, Shimane M (1997) Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to vaccinia virus B1R kinase. Genomics 45: 327–331.J. NezuA. OkuMH JonesM. Shimane1997Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to vaccinia virus B1R kinase.Genomics45327331
- 63. Vega FM, Gonzalo P, Gaspar ML, Lazo PA (2003) Expression of the VRK (vaccinia-related kinase) gene family of p53 regulators in murine hematopoietic development. FEBS Lett 544: 176–180.FM VegaP. GonzaloML GasparPA Lazo2003Expression of the VRK (vaccinia-related kinase) gene family of p53 regulators in murine hematopoietic development.FEBS Lett544176180
- 64. Putcha GV, Le S, Frank S, Besirli CG, Clark K, et al. (2003) JNK-mediated BIM phosphorylation potentiates BAX-dependent apoptosis. Neuron 38: 899–914.GV PutchaS. LeS. FrankCG BesirliK. Clark2003JNK-mediated BIM phosphorylation potentiates BAX-dependent apoptosis.Neuron38899914
- 65. Lei K, Davis RJ (2003) JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis. Proc Natl Acad Sci U S A 100: 2432–2437.K. LeiRJ Davis2003JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis.Proc Natl Acad Sci U S A10024322437
- 66. Puthalakath H, Strasser A (2002) Keeping killers on a tight leash: transcriptional and post-translational control of the pro-apoptotic activity of BH3-only proteins. Cell Death Differ 9: 505–512.H. PuthalakathA. Strasser2002Keeping killers on a tight leash: transcriptional and post-translational control of the pro-apoptotic activity of BH3-only proteins.Cell Death Differ9505512
- 67. Cheng EH, Wei MC, Weiler S, Flavell RA, Mak TW, et al. (2001) BCL-2, BCL-X(L) sequester BH3 domain-only molecules preventing BAX- and BAK-mediated mitochondrial apoptosis. Mol Cell 8: 705–711.EH ChengMC WeiS. WeilerRA FlavellTW Mak2001BCL-2, BCL-X(L) sequester BH3 domain-only molecules preventing BAX- and BAK-mediated mitochondrial apoptosis.Mol Cell8705711
- 68. Zong WX, Lindsten T, Ross AJ, MacGregor GR, Thompson CB (2001) BH3-only proteins that bind pro-survival Bcl-2 family members fail to induce apoptosis in the absence of Bax and Bak. Genes Dev 15: 1481–1486.WX ZongT. LindstenAJ RossGR MacGregorCB Thompson2001BH3-only proteins that bind pro-survival Bcl-2 family members fail to induce apoptosis in the absence of Bax and Bak.Genes Dev1514811486
- 69. Black DS, Bliska JB (1997) Identification of p130Cas as a substrate of Yersinia YopH (Yop51), a bacterial protein tyrosine phosphatase that translocates into mammalian cells and targets focal adhesions. EMBO J 16: 2730–2744.DS BlackJB Bliska1997Identification of p130Cas as a substrate of Yersinia YopH (Yop51), a bacterial protein tyrosine phosphatase that translocates into mammalian cells and targets focal adhesions.EMBO J1627302744
- 70. Haslinger-Loffler B, Kahl BC, Grundmeier M, Strangfeld K, Wagner B, et al. (2005) Multiple virulence factors are required for Staphylococcus aureus-induced apoptosis in endothelial cells. Cell Microbiol 7: 1087–1097.B. Haslinger-LofflerBC KahlM. GrundmeierK. StrangfeldB. Wagner2005Multiple virulence factors are required for Staphylococcus aureus-induced apoptosis in endothelial cells.Cell Microbiol710871097
- 71. Weglarczyk K, Baran J, Zembala M, Pryjma J (2004) Caspase-8 activation precedes alterations of mitochondrial membrane potential during monocyte apoptosis induced by phagocytosis and killing of Staphylococcus aureus. Infect Immun 72: 2590–2597.K. WeglarczykJ. BaranM. ZembalaJ. Pryjma2004Caspase-8 activation precedes alterations of mitochondrial membrane potential during monocyte apoptosis induced by phagocytosis and killing of Staphylococcus aureus.Infect Immun7225902597
- 72. Nilsdotter-Augustinsson A, Wilsson A, Larsson J, Stendahl O, Ohman L, et al. (2004) Staphylococcus aureus, but not Staphylococcus epidermidis, modulates the oxidative response and induces apoptosis in human neutrophils. APMIS 112: 109–118.A. Nilsdotter-AugustinssonA. WilssonJ. LarssonO. StendahlL. Ohman2004Staphylococcus aureus, but not Staphylococcus epidermidis, modulates the oxidative response and induces apoptosis in human neutrophils.APMIS112109118
- 73. Schnaith A, Kashkar H, Leggio SA, Addicks K, Kronke M, et al. (2007) Staphylococcus aureus subvert autophagy for induction of caspase-independent host cell death. J Biol Chem 282: 2695–2706.A. SchnaithH. KashkarSA LeggioK. AddicksM. Kronke2007Staphylococcus aureus subvert autophagy for induction of caspase-independent host cell death.J Biol Chem28226952706
- 74. Barz C, Abahji TN, Trulzsch K, Heesemann J (2000) The Yersinia Ser/Thr protein kinase YpkA/YopO directly interacts with the small GTPases RhoA and Rac-1. FEBS Lett 482: 139–143.C. BarzTN AbahjiK. TrulzschJ. Heesemann2000The Yersinia Ser/Thr protein kinase YpkA/YopO directly interacts with the small GTPases RhoA and Rac-1.FEBS Lett482139143
- 75. Mills SD, Boland A, Sory MP, van der Smissen P, Kerbourch C, et al. (1997) Yersinia enterocolitica induces apoptosis in macrophages by a process requiring functional type III secretion and translocation mechanisms and involving YopP, presumably acting as an effector protein. Proc Natl Acad Sci U S A 94: 12638–12643.SD MillsA. BolandMP SoryP. van der SmissenC. Kerbourch1997Yersinia enterocolitica induces apoptosis in macrophages by a process requiring functional type III secretion and translocation mechanisms and involving YopP, presumably acting as an effector protein.Proc Natl Acad Sci U S A941263812643
- 76. Nigg EA (2001) Mitotic kinases as regulators of cell division and its checkpoints. Nat Rev Mol Cell Biol 2: 21–32.EA Nigg2001Mitotic kinases as regulators of cell division and its checkpoints.Nat Rev Mol Cell Biol22132
- 77. Pearson G, Robinson F, Beers Gibson T, Xu BE, Karandikar M, et al. (2001) Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr Rev 22: 153–183.G. PearsonF. RobinsonT. Beers GibsonBE XuM. Karandikar2001Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions.Endocr Rev22153183
- 78. Lu KP, Liou YC, Vincent I (2003) Proline-directed phosphorylation and isomerization in mitotic regulation and in Alzheimer's Disease. Bioessays 25: 174–181.KP LuYC LiouI. Vincent2003Proline-directed phosphorylation and isomerization in mitotic regulation and in Alzheimer's Disease.Bioessays25174181
- 79. Beltramini AM, Mukhopadhyay CD, Pancholi V (2009) Modulation of cell wall structure and antimicrobial susceptibility by a Staphylococcus aureus eukaryote-like serine/threonine kinase and phosphatase. Infect Immun 77: 1406–1416.AM BeltraminiCD MukhopadhyayV. Pancholi2009Modulation of cell wall structure and antimicrobial susceptibility by a Staphylococcus aureus eukaryote-like serine/threonine kinase and phosphatase.Infect Immun7714061416
- 80. Jiang L, He L, Fountoulakis M (2004) Comparison of protein precipitation methods for sample preparation prior to proteomic analysis. J Chromatogr A 1023: 317–320.L. JiangL. HeM. Fountoulakis2004Comparison of protein precipitation methods for sample preparation prior to proteomic analysis.J Chromatogr A1023317320
- 81. Sambrook J, Fritsch EF, T M (1989) Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory Press. J. SambrookEF FritschM. T1989Molecular cloning: a laboratory manual.New YorkCold Spring Harbor Laboratory Press
- 82. Kreiswirth BN, Lofdahl S, Betley MJ, O'Reilly M, Schlievert PM, et al. (1983) The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature 305: 709–712.BN KreiswirthS. LofdahlMJ BetleyM. O'ReillyPM Schlievert1983The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage.Nature305709712
- 83. Norrander J, Kempe T, Messing J (1983) Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26: 101–106.J. NorranderT. KempeJ. Messing1983Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.Gene26101106