Archaeopteryx is the oldest and most primitive known bird (Avialae). It is believed that the growth and energetic physiology of basalmost birds such as Archaeopteryx were inherited in their entirety from non-avialan dinosaurs. This hypothesis predicts that the long bones in these birds formed using rapidly growing, well-vascularized woven tissue typical of non-avialan dinosaurs.
We report that Archaeopteryx long bones are composed of nearly avascular parallel-fibered bone. This is among the slowest growing osseous tissues and is common in ectothermic reptiles. These findings dispute the hypothesis that non-avialan dinosaur growth and physiology were inherited in totality by the first birds. Examining these findings in a phylogenetic context required intensive sampling of outgroup dinosaurs and basalmost birds. Our results demonstrate the presence of a scale-dependent maniraptoran histological continuum that Archaeopteryx and other basalmost birds follow. Growth analysis for Archaeopteryx suggests that these animals showed exponential growth rates like non-avialan dinosaurs, three times slower than living precocial birds, but still within the lowermost range for all endothermic vertebrates.
The unexpected histology of Archaeopteryx and other basalmost birds is actually consistent with retention of the phylogenetically earlier paravian dinosaur condition when size is considered. The first birds were simply feathered dinosaurs with respect to growth and energetic physiology. The evolution of the novel pattern in modern forms occurred later in the group's history.
Citation: Erickson GM, Rauhut OWM, Zhou Z, Turner AH, Inouye BD, Hu D, et al. (2009) Was Dinosaurian Physiology Inherited by Birds? Reconciling Slow Growth in Archaeopteryx. PLoS ONE 4(10): e7390. https://doi.org/10.1371/journal.pone.0007390
Editor: Robert DeSalle, American Museum of Natural History, United States of America
Received: June 6, 2009; Accepted: September 16, 2009; Published: October 9, 2009
Copyright: © 2009 Erickson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research was generously funded by an DFG grant RA 1012/4 to OWMR, NSF DBI 0446224 and EAR 04418649 grants to GME, an NSF ATOL 0228693 grant to MAN, and the Major Basic Research Projects [2006CB806400] of MST of China to ZZ. The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The genesis of birds was a key event in vertebrate history. The descendants of these animals have dominated aerial niches from the Late Mesozoic until today, where they are the most speciose amniote clade (∼10,000 species) , . Skeletal, feather, and behavioral evidence conclusively place birds within the evolutionary radiation of theropod dinosaurs, rightly making birds the only living dinosaurs –. Growth patterns, revealed through long bone osteohistology can be traced phylogenetically through this transition and used as a proxy for inferring physiological changes. Non-avialan dinosaur bones show a characteristic well-vascularized, rapidly formed woven-fibered matrix that is often interrupted by growth lines – (Figure 1). Similar broad-scale histological attributes have been reported in Mesozoic bird lineages close to the base of the avialan tree – (Figure 1). This has contributed to the paradigm that dinosaurian physiology is ancestral for Avialae as a whole. (Although, whether that condition was comparable to living birds is a subject of debate , –.)
(A) The femoral microstructure of the small alvarezsaurrid, Shuvuuia deserti (IGM 100–99) is compared to the tibial histology (B) of the Early Cretaceous avialan, Confuciusornis sanctus (IVPP V11521). Both show well-vascularized bone owing to the presence of numerous primary vascular canals (large black structures), and woven fibered matrix characterized by oblong, randomly oriented osteocyte lacunae (numerous small black structures). Arrows point to growth lines in the form of a line of arrested growth (left) and an annulus (right). Scale bars = 0.5 mm (A) and 0.1 mm (B).
We used dissecting microscopy to directly examine the long bone periosteal and fracture surfaces spanning the entire size range of Archaeopteryx (Eichstätt, Munich, Ottmann & Steil, London, and Solnhofen specimens; Table 1), the oldest known bird . (Note: we follow Chiappe's (2) interpretation that all specimens are referable to a single species, Archaeopteryx lithographica; see Materials and Methods.) In the two largest individuals delamination of cortical bone consistent with growth line interfaces were found, as anticipated (Figure 2). However, all specimens unexpectedly showed exceptionally sparse longitudinal vascularization visible on the periostal surfaces and within the semi-transparent bones (Figure 3). Furthermore, transversely running fracture faces showed a circumferential fabric characteristic of the dense lamellar or parallel-fibered bone-types of living non-dinosaurian reptiles .
Femora, tibiae, fibulae, pubes, and gastralia from the Solnhofen specimen (BMMS 500), are shown. The brown cortical layer of the left femur (inset box) is superior to the light gray underlying bone layer that is exposed where the former flaked-off. This is consistent with a growth line interface. These thin, hypermineralized osseous layers partition the more fibrous zonal tissues and act as planes of weakness where exfoliation can occur.
(A) Tibial diaphysis from the smallest known Archaeopteryx, the Eichstät specimen (JM 2257) showing sparse longitudinal vascularization in the form of parallel striae. Comparable immature texture and patterning is found in the major long bones throughout the Archaeopteryx growth series. For example, it is seen in the tibiae of the moderately larger Munich specimen (BSPG 1999 I 50) shown in (B), and in the largest known individual (The Solnhofen specimen, BMMS 500) shown in (C). Scale bars = 1.5 mm.
These observations conflict with the hypothesis that dinosaurian-type growth physiology is primitive for the first birds. Alternative explanations are: 1) Archaeopteryx grew aberrantly, 2) basalmost avialan growth was substantially decelerated, perhaps in response to the reallocation of resources from growth to powered flight, or 3) that current phylogenetic resolution of histological character states are insufficient to correctly infer the basalmost avialan condition.
To test these hypotheses we used a comparative phylogenetic approach. We were given the opportunity to study the long bone histology of a juvenile specimen of Archaeopteryx (The Munich Specimen, BSPG 1999 I 50; Figure 4) from which we describe the taxon's peculiar osseous microstructure. We also sampled the successive crownward lineages, Jeholornis (another very primitive long-tailed avialan; ), and Sapeornis (the primitive short-tailed avialan that retains three fingers and teeth; ), to provide broader representation of avialan histology in truly basal lineages (Table 1). (Initial studies utilized the histology of Rahonavis, that was once thought to occupy a similar position ,  but is now considered a non-avialan dinosaur , ; and the birds Confuciusornis, Enantiornithes, and Patagopteryx , , ,  that are now placed well crownward from the basal avialan node .)
Cortical samples were extracted from the fracture faces of broken elements. (A) Main slab with arrows showing where femoral samples were extracted. (B) Counterslab showing where the fibula was sampled. Scale bar = 5 cm.
Interpreting our basal avialan findings in a phylogenetic context spanning the avialan transition also necessitated broader histological sampling of the proximate outgroup maniraptoran clades, Oviraptorosauria and Deinonychosauria (, ; Figure S1). Initial examinations across the avialan transition had either no samples ascribed to these clades, or just one representative – Troodon ,  or Unenlagia . The large size of these deinonychosaurs (∼50–100 kg; ) is a potential problem for predicting the basal condition for Avialae as scaling has a strong influence on histological pattern , –. Dinosaurs are no exception , ,  and it has recently been shown that basal non-avialan deinonychosaurs were actually miniaturized (∼1 kg) and this size is primitive for Paraves (Deinonychosauria + Avialae) . These data demonstrate the need for broader sampling (both phylogenetically and inclusive of scale) among outgroup clades.
Finally, because tissue formation rates can reveal developmental rates ,  we used the growth series for Archaeopteryx to independently test the hypothesis that non-avialan dinosaurian growth rates were ancestral for birds. The results were compared to expectations for same-sized non-avialan dinosaurs  and extant precocial birds .
Our analysis reveals that femoral histological patterning is correlated with size, and forms a scale-dependent continuum within Maniraptora (Figure 5). Moving from larger to smaller forms among the non-avialan dinosaurs, vascular complexity simplifies (plexiform to circumferential to longitudinal/reticular to longitudinal) and randomness in bone fiber orientation diminishes (woven to parallel-fibered). Deep cortical growth lines are variably present, but up to five exist in larger forms and no more than two in the smallest taxa. Femoral porosity decreases (11.32 to 1.26%) (Figure 6). Most notable from our analysis is that the smallest deinonychosaur taxon we sampled, Mahakala, (76 mm femoral length, ) lacks fibro-lamellar bone, but rather has reptilian-like parallel-fibered bone and shows sparse longitudinal vascularization (Figure 5).
The specimens are viewed with polarized microscopy. Each is oriented with the periosteal surface towards the top of the figure. Five histological character suites (I.–V.) are present. The attributes composing each suite appear below the figure and are denoted by (black and/or gray horizontal bars). For example: Maniraptoran Type I shows longitudinal vascularizaton, parallel-fibered matrix, and low porosity. Lines stemming from the bottom the histological images trace to the representative histological suites for each taxon. The character suites show intracladal scale dependence with respect to femoral length. The basal avialans conform to expectations of same-sized non-avialan maniraptoran outgroups. Phylogenetic hypothesis based on ref. 22.
Intracortical transverse plane porosity from the histological sections was quantified (See Materials and Methods). These data are plotted with respect to femoral length. Data for non-avialan taxa are denoted by black diamonds. The regression equation describes only their distribution. The blue lines bound the 95% confidence interval. The red diamonds represent the basalmost avialans studied here. Note: their data are encompassed by the maniraptoran confidence interval and that for sister taxon Deinonychosauria (not shown).
Like Mahakala, histological analysis revealed that the femoral diaphysis of Archaeopteryx (BSPG 1999 I 50) is almost entirely composed of parallel-fibered bone with longitudinally oriented vascular canals (Figure 7). The latter are even more sparsely distributed (porosity 0.68%). Deep intracortical growth lines (lines of arrested growth and/or annuli) are absent in this young specimen . A thin layer of endosteal bone is locally present. Haversian bone tissue was not found. The fibular metaphysis showed comparable primary bone histology, and compacted endosteal bone. The longitudinal vascular canals pervade throughout the semi-translucent cortices of the long bones. Partially formed canals manifest themselves as the conspicuous grooves seen at the periosteal surfaces (Figure 7).
(A) Parallel-fibered bone is found throughout the cortex as shown by the flattened, circumferentially oriented, lenticular osteocyte lacunae (tiny black structures) and matted bone fabric (lower left). (B) Primary longitudinal vascular canals are few (large black circular structures). These are occasionally found incompletely formed at the periosteal surface (arrow) and are responsible for the fibrous surface texture of the elements and long striae seen deep within the bones of all known individuals. Scale bar = 0.75 mm.
Jeholornis shows parallel-fibered bone and longitudinal vascularization like Archaeopteryx and the small deinonychosaur Mahakala (Figure 8). Woven bone is only locally present where the cortex is thickest. Femoral porosity is greater (1.7%) in this slightly larger taxon than Archaeopteryx. A single deep intracortical growth line is present in the largest individual (Figure 8). The even larger avialan Sapeornis shows primarily woven bone matrix and a mix of longitudinal and reticular vascularization similar to small oviraptorosaurs (e.g., Caudipteryx) and similar-sized deinonychosaurs (e.g., IGM 100/1323). Femoral porosity (1.3%) is also greater than that of Archaeopteryx. A single deep cortical growth line bounded by parallel-fibered bone separates growth zones in this sub-adult specimen (Figure 8).
(A) In Jeholornis (IVPP 13353), parallel-fibered bone matrix similar to that of Archaeopteryx makes up the cortex. However porosity is greater as in Mahakala. A growth line that locally varies between a line of arrested growth and an annulus is shown (arrow). (B) In the larger Sapeornis (LPM B00166), the matrix is primarily woven-fibered and shows a mix of longitudinal and reticular vascularization. Avascular parallel-fibered bone brackets a line of arrested growth (arrow) in this sub-adult specimen. Scale bar = 0.15 mm
The histological patterning (Figure 5) and porosity (Figure 6) of the basalmost birds Archaeopteryx, Jeholornis, and Sapeornis conforms to expectations based on the maniraptoran and deinonychosaur size continua. This supports the presence of a more complex size-dependent non-avialan histology but one that is nonetheless primitive for Avialae. Our results demonstrate that by the paravian diversification, when Archaeopteryx-sized dinosaurs evolved, parallel-fiber bone and reduced porosity was expressed.
Our growth analysis (see Materials and Methods) suggests that the known Archaeopteryx specimens span approximately 428 days in development (Figure 9). Maximal growth rates were in the range of 1.87 to 2.2 g/day across that span. These rates compare favorably with the expectations of 1.83 to 1.87 g/day for a same-sized non-avialan dinosaur (Figure 9) . Asymptotic body mass was between approximately 895 and 928 g and somatic maturity appears to have been reached during the second to third years of life (Figure 9). The same timing is predicted for Jeholornis and Sapeornis based upon growth zone counts. These values are also consistent with expectations for outgroup non-avialan dinosaurs of comparable size .
(Left) The size and estimated age for all ten specimens are depicted. The growth curves are based upon age and size estimates (diamonds) for the eight specimens where femoral length is known. The dashed line represents the best fit for the unconstrained statistical analysis with hatchling and adult size undefined. The solid line represents the best fit when hatchling and adult size are constrained. (Right) The maximal growth rates from these analyses (1.87–2.2 g/day; hollow diamond) fit expectations (1.83–1.87 g/day; ) for same-sized non-avialan dinosaurs (solid line) – animals that grew like slow growing endotherms, here compared to marsupials (M). The Archaeopteryx estimates are three times lower than typical rates for extant precocial land birds (5.7 g/day; P], 15 times lower than alticial land birds (28.6 g/day; A), and four times higher than typical rates for extant reptiles (0.46 g/day; R) . Specimens designations: Ei = Eichstäat, Mu = Munich, 8th = 8th Exemplar, Te = Teyler, Th = Thermopolis, Be = Berlin, Ma = Maxberg, O&S = Exemplar der Familien Ottmann & Steil, Lo = London, So = Solnhofen.
Our discovery that the long bones of Archaeopteryx are composed of slow growing, reptilian grade, parallel-fibered bone is surprising. Well-vascularized woven bone was expected. The finding of the same peculiar matrix and vascular pattern in the slightly more derived bird Jeholornis indicates that the Archaeopteryx histology was not aberrant, but is typical of the basal avialan condition. The moderate increase in porosity in the birds Jeholornis and Sapeornis is concurrent with increased size, as is the predominance of woven matrix in the latter taxon.
Collectively the histological suites seen in the basalmost birds match expectations for same-sized taxa in the maniraptoran histological continuum. They provide compelling evidence that the non-avialan dinosaur growth pattern was inherited in totality and is typical of basal birds nearest to the Archaeopteryx node. This adds a physiological attribute to the long list of anatomical and behavioral attributes showing that birds are dinosaurs and that many early birds were typically very dinosaur-like –. Furthermore, it underscores that it is imperative to accommodate scaling when making phylogenetically grounded inferences about evolutionary changes in growth physiology through osseous histology .
The absence of available small outgroup taxa prior to this analysis likely hindered the detection of the precise relationship between body size and histology among paravians. Optimizing histological type within a broad sampling of maniraptoran theropods encompassing a diversity of sizes reveals multiple occurrences of the parallel-fiber suite within Paraves (Figure S2). Parallel-fiber bone growth, therefore, is not unique to Avialae. This is independent evidence that early avialan growth was neither novel, nor aberrant, but rather one end on the primitive histological continuum detailed above.
The ramifications of these findings for early avialan life history and physiology are considerable. For instance it has been recently speculated based on tissue characteristics (e.g. growth lines , , vascularization and fiber patterning , , ), broad size distribution (in Archaeopteryx; , ), and apparent age cohorts (in Confuciusornis; ) that growth in the first birds was slower than most same-sized living birds (Neornithes) which mature in just days to weeks . However the estimates for the fossil birds range broadly from as little as two months to unspecified numbers of years.
We find more constrained estimates, whereby somatic maturity in the three basalmost lineages of birds occurred during the second and third years of life. Evidence for this includes: 1) the finding of exceptionally slow growing bone tissues suggesting prolonged development relative to living birds, 2) estimates that Archaeopteryx somatically matured in no less than 970 days (Figure 9) (there were 375 days in a Late Jurassic year; ), 3) growth lines that not only bridge the paravian record, but attest to developmental stoppages and multi-year development among the basalmost birds, and 4) histological character suites that meet expectations for same-sized non-avialan theropods known to mature in no less than two years .
Aside from anatomical differences such as long bony tails, clawed hands, and teeth, the slow development of the first birds would have made their biology appear unfamiliar to ornithologists. In most comparable-sized volant birds, juveniles and sub-adult sized animals are present for only a small fraction of the year as growth rates are remarkably rapid , . In basalmost birds, actively growing juveniles would change imperceptibly week to week, and sub-adults would be present year-round. This slow development might also have prolonged their time to fledging (However what the volant capability was of early birds like Archaeopteryx remains conjectural , , –.) Among same-sized living precocial birds individuals are typically earthbound for 3–6 weeks before becoming volant. Assuming that the age of the smallest Archaeopteryx specimens approximates when these animals first took flight across the Solnhofen lagoons, we can deduce that this milestone would have occurred no later than about 18 weeks (Figure 9).
Accurately estimating the adult size of basal birds is critical for studies of taxonomy, development, comparative physiology, ecology, heterochrony, flight biomechanics, and collectively the evolution of avialan success. This measure is known for Jeholornis and Sapeornis from specimens showing extensive neurocentral and braincase fusion. However for Archaeopteryx, our best representative of the initial avialan condition, adult size has remained ambiguous . These animals have been variably described in the professional and lay public literature as robin, grackle, pigeon, magpie, small gull, crow, and chicken-sized. Quantified estimates of body mass have varied between 200 g and 600 g , . Nevertheless, allometric scaling and skeletal fusion analyses have inferred that none of the known specimens are somatically mature and allude to a quantitatively larger endpoint . Our histological and textural examinations showing that immature bone is still prevalent in the largest specimens strongly supports this (Figure 3), as do inferences from the growth curve that none are asymptotic individuals (Figure 9). The deinonychosaur histological continuum provides a new means to estimate the taxon's upper bound. It is unlikely that Archaeopteryx had a femoral length exceeding 75 mm as a shift to a more porous histology and perhaps woven matrix would have been evident (Figure 5). Femoral lengths of 70–75 mm translate to adult mass estimates of 822–1009 g, which can be analogized to the common raven (Corvus corax) for size .
Physiologically modern birds are endothermic and characteristically have among the highest relative growth rates and basal metabolic rates among extant animals . Maximal growth rates strongly correlate with basal metabolic rate , . Erickson and colleagues , ,  have shown that whole body maximal growth rates for non-avialan dinosaurs are within the range expected for endotherms. These rates however are not typical of living birds. Rather they are nearer the lower bound for endotherms, and are more in line with animals such as marsupials , . (Notably, kiwi (Apteryx), the living birds that show relative growth rates  most closely approaching the non-avialan dinosaurian condition, also have exceptionally low metabolic rates .) Since basal avialan body size, bone histology, and growth rates (as evidenced by Archaeopteryx and assumed for Jeholornis and Sapeornis through continuum matching) were retained from dinosaurian ancestry we can infer that these animals had a similar metabolism. This suggests that the initial conquest of the air was achieved using lower metabolic rates than are characteristic of today's avian flyers. It appears that the closest non-avialan relatives of birds were physiologically preadapted for powered flight and only anatomical adaptations were involved when birds first ventured into the air.
The stereotypical ideal of Archaeopteryx as a physiologically modern bird with a long tail and teeth has come under scrutiny in the last decade –. The present findings, based on the first direct histological evidence for Archaeopteryx and other basalmost birds, shows incontrovertibly that these animals were very primitive, similar to their non-avialan dinosaur precursors – Archaeopteryx was simply a feathered and presumably volant dinosaur. Theories regarding the subsequent steps that lead to the modern avian condition need to be reevaluated in a scale dependent manner to help understand what is turning out to be a complex evolutionary story.
Materials and Methods
The taxonomic history of Archaeopteryx is complex, with almost every specimen having been assigned to a different species or even genus at some time (see  and , for recent reviews). However, Houck et al.  and Senter and Robins  argued that differences in proportions in nearly all specimens are explained by allometric developmental scaling. Other differences might be explained as sexual, or individual variation . Thus, pending a more detailed revision of the taxonomy of Archaeopteryx, we agree with Chiappe  that all specimens are referable to a single species, Archaeopteryx lithographica.
The Munich specimen of Archaeopteryx (BSPG 1999 I 50) is preserved as slab and counterslab (Figure 4). From the fracture faces of the long bones we extracted minute cortical chips spanning the periosteal to endosteal surfaces (Figure 4; Figure S3). These included diaphyseal samples from the femur (the standard for comparative growth analyses in dinosaurs ,  and a metaphyseal/diaphyseal sample of the fibula (an element that commonly preserves the majority of the growth record in theropods , ). Transverse plane histological slides were made and viewed with polarizing and reflectance microscopy and the microstructure described . The periosteal fabric (seen in actual specimens and in research casts and photos, Table 1) and vascular patterning visible throughout the semi-translucent bones and on long bone fracture faces show that the juvenile histology of the Munich specimen is found throughout the growth series (Figure 3). This is not unexpected because all known Archaeopteryx are young individuals spanning a developmentally very limited range .
Diaphyseal histological sections and measurements of femora were then made for: the basal avialans Jeholornis prima and Sapeornis chaochengensis; the oviraptorids Citipati osmolskae, Conchoraptor gracilis, and Caudipteryx zoui; and the deinonychosaurs: Mahakala omnogovae, Utahraptor ostrommaysi, Deinonychus antirrhopus, Velociraptor mongoliensis, Troodon formosus, Byronosaurus jaffei, and two undescribed Mongolian troodontids (Table 1). The histology for deep cortical primary bone formed during early development was described. Intracortical transverse plane porosity was quantified using NIH Image J (Version 1.41o; National Institutes of Health, Bethesda, Maryland). Character mapping and phylogenetic optimization of these data was conducted using TNT version 1.0  on a phylogeny adapted from Turner et al.  for Coelurosauria. From this we: 1) inferred the effects of scaling on histological patterning with femoral length as a size standard, 2) predicted the primitive character state for Paraves, and 3) inferred whether evolutionary changes occurred across the transition.
Because the Archaeopteryx specimens are young individuals that fortuitously span middle development  it was possible to estimate age differences between individuals and speculate on Archaeopteryx growth rate during the exponential stage of development. This was achieved using neontologically derived tissue formation rates, similar to the means described by Sander and Tückmantel  for aging sauropods. Differences in antero-posterior femoral midshaft diameter relative to the Munich specimen were determined throughout the growth series. (In specimens with uncrushed femora the ratio of femur length to diameter ranges from 13.47–13.67 across ontogeny (Eichstätt-London specimens). The mean ratio of 13.62 was used to deduce the diameter for the Solnhofen specimen (BMMS 500).) Radial tissue formation rates derived from femora in living birds ,  were divided into these measurements and the age differences deduced. Positive correlations between tissue formation rates and body size among homologous elements are evident in birds , . Sustained radial formative rates for the matching histological type to Archaeopteryx avian femora is 2.5 um/day in Archaeopteryx-sized birds (e.g. 880 g Mallard ducks; Anas platyrhynchos). Rates as high as 4.2 um/day occur in very large taxa (e.g. 90 kg Ostrich, Struthio camelus) for femora showing the same vascularizaton pattern, however the matrix is woven-fibered. The former value was used.
Body mass of the large Solnhofen specimen (BMMS 500) was determined using the femoral and tibial regression equations for galliforms (“poor flying birds”) from Alexander . The Developmental Mass Extrapolation scaling principle ,  was used to infer specimen masses and coupled with the age differences to model growth rates throughout development. We first used maximum likelihood to fit a logistic equation with normal error distribution to the growth data for the eight Archaeopteryx specimens for which femoral length is known (mass = (a*exp[b*age])/(c+exp[b*age]) + d, a = 752.6, b = 0.0118, c = 274.2, d = 145.7, r2 = 0.964). This growth curve projects an estimated hatchling mass of 148 g, an adult mass of 898 g, and a maximal daily growth rate of 2.2 g/day. Because the projected hatchling mass from this maximum likelihood estimate is biologically implausible, we used comparisons with other taxa and our histological observations to construct informative prior distributions for hatchling and adult mass and calculated a Bayesian estimate of Archaeopteryx growth. Specifically, a 39.6 g estimate for a full-term egg mass was obtained from the regression analysis of Blueweiss . A similar size of 53 g was predicted using the femoral dimensions for a near term Gobipteryx minuta (IGM 100/1291) scaled up to account for the larger size of Archaeopteryx. The femoral histological continuum [see results] predicts that Archaeopteryx had a femoral length that was less than 75 mm otherwise the histology seen in larger paravians would have been expressed. The largest known Archaeopteryx is 683 g and the slightly larger paravians, which have a modified histology owing to scale dependence, place full adult size at less than 1009 g. Based on these estimates we used informative priors for hatchling mass of approximately 43±10 g (d = Normal[20, sd = 5]) and an adult mass of 895±165 g (a = Normal[875, sd = 82.5]). This prior distribution for adult mass allows for a large range of plausible adult sizes, whereas the estimated hatchling size is constrained to a smaller range. Maximum daily growth rate was unconstrained, as we used uninformative priors for parameters b and c(Gamma [0.01, 0.01]). The Bayesian analysis provided similar results to the unconstrained maximum likelihood fit, but more plausible posterior estimates of hatchling size (41.4 g) and adult size (928 g) and a slightly lower estimate of maximal growth rate at 1.87 g/day (a = 907±71.5, b = 0.0082±0.0008, c = 43.6±25, d = 21.1±5, means±sd, r2 = 0.942). The results of these growth analyses were compared with predictions for same-sized non-avialan dinosaurs and extant precocial birds.
Statistical analyses were conducted using R 2.9.0 (R Development Core Team; R Foundation for Statistical Computing, Vienna, Austria) and WinBUGS 1.4.3 .
Although it is plausible that hatchling Archaeopteryx initially grew using woven bone as has been shown in enantiornithines , , this would have had negligible affect on the maximal growth rate estimates made here since the studied specimens span the entire exponential stage of development (Figure 9). Furthermore, very little development occurred prior to the size obtained by the Eichstätt specimen (JM 2257), just 78 grams. So individual longevity estimates would be diminished by no more than approximately 24–30 days from initial woven bone formation. Conversely, growth stoppages in the largest Archaeopteryx specimens would have contributed to moderately higher longevity estimates for these specimens and slightly lower maximal growth rate estimates. This would bolster the overall conclusion that basal avialans were not growing like living precocial birds (see results).
Preferred topology for Maniraptora used in the present study. Tree topology is derived from the phylogenetic analyses of Turner et al. . Taxa listed in bold are those for which we obtained histological data.
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Optimization of fiber types on femoral length. Optimization of bone fiber-type on maniraptoran phylogeny. Woven bone fiber-type is widespread among maniraptorans. Note that minimally two, but perhaps three, independent acquisitions of parallel fiber bone occurred in the small paravians Mahakala, Archaeopteryx, and Jeholornis.
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Long bone chips extracted from the Munich Archaeopteryx (BSP 1999 I 50).
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We thank Renate Liebreich for her skilled removal of the bone chips and Angela Milner, Paul Barrett, Brooks Britt, Rod Sheetz, David Varricchio, Karl Mehling, Jack Horner, Martina Köbl-Ebert, Ute Grimm, Birgitt Ott, Manfred Schneider, and Martin Röper for access to specimens in their care. Jacques Gauthier and Peter Makovicky kindly provided access to their Archaeopteryx photo archives. Paul Gignac offered useful comments to the manuscript. Mick Ellison and Helmut Tischlinger provided photographic assistance and Ken Womble and Frank Ippolito assisted with the graphics.
Conceived and designed the experiments: GME OR MN. Performed the experiments: GME OR MN. Analyzed the data: GME OR AT BDI DH. Contributed reagents/materials/analysis tools: GME OR ZZ DH. Wrote the paper: GME OR ZZ AT BDI MN. Conducted the histological analyses: GME OR.
- 1. Gill FB (2007) Ornithology, 3rd ed. New York: WH Freeman. 758 p.FB Gill2007Ornithology, 3rd ed.New YorkWH Freeman758
- 2. Chiappe LM (2007) Glorified Dinosaurs: The Origin and Early Evolution of Birds. Sydney: University of New South Wales Press. 263 p.LM Chiappe2007Glorified Dinosaurs: The Origin and Early Evolution of Birds.SydneyUniversity of New South Wales Press263
- 3. Chiappe LM, Witmer LM, editors. (2002) Mesozoic Birds: Above the Heads of Dinosaurs. Berkeley: University of California Press. 520 p.LM ChiappeLM Witmer2002Mesozoic Birds: Above the Heads of Dinosaurs.BerkeleyUniversity of California Press520
- 4. Gauthier J, Gall LF, editors. (2001) New Perspectives on the Origin and Early Evolution of Birds. New Haven: Peabody Museum of Natural History. 613 p.J. GauthierLF Gall2001New Perspectives on the Origin and Early Evolution of Birds.New HavenPeabody Museum of Natural History613
- 5. Chinsamy A (2005) The Microstructure of Dinosaur Bone: Deciphering Biology with Fine-Scale Techniques. Baltimore: Johns Hopkins Press. 195 p.A. Chinsamy2005The Microstructure of Dinosaur Bone: Deciphering Biology with Fine-Scale Techniques.BaltimoreJohns Hopkins Press195
- 6. Erickson GM (2005) Assessing dinosaur growth patterns: A microscopic revolution. Trends Ecol Evol 20: 677–684.GM Erickson2005Assessing dinosaur growth patterns: A microscopic revolution.Trends Ecol Evol20677684
- 7. Padian K, Horner JR, Ricqlés A de (2004) Growth in small dinosaurs and pterosaurs: The evolution of archosaurian growth strategies. J Vert Paleo 24: 555–571.K. PadianJR HornerA de Ricqlés2004Growth in small dinosaurs and pterosaurs: The evolution of archosaurian growth strategies.J Vert Paleo24555571
- 8. Chinsamy A (2002) Bone microstructure of early birds. In: Chiappe LM, Witmer LM, editors. Mesozoic Birds: Above the Heads of Dinosaurs. Berkeley: University of California Press. pp. 421–431.A. Chinsamy2002Bone microstructure of early birds.LM ChiappeLM WitmerMesozoic Birds: Above the Heads of DinosaursBerkeleyUniversity of California Press421431
- 9. Ricqlés A de, Padian K, Horner JR, Lamm E-Th, Myrhvold N (2003) Osteohistology of Confuciusornis sanctus [Theropoda: Aves] J Vert Paleo 23: 373–386.A de RicqlésK. PadianJR HornerE-Th LammN. Myrhvold2003Osteohistology of Confuciusornis sanctus [Theropoda: Aves]J Vert Paleo23373386
- 10. Zhang F, Hou L, Ouyang L (1998) Osteological microstructure of Confuciusornis: Preliminary Report. Vert PalAsia 36: 126–135.F. ZhangL. HouL. Ouyang1998Osteological microstructure of Confuciusornis: Preliminary Report.Vert PalAsia36126135
- 11. Cambra-Moo O, Delgado Buscalioni Á, Cubo J, Castanet J, Marie-Madeleine Loth M-M, et al. (2006) Histological observations of enantiornithine bone [Saurischia, Aves] from the Lower Cretaceous of Las Hoyas [Spain]. Comptes Rendus Palevol 5: 685–691.O. Cambra-MooÁ. Delgado BuscalioniJ. CuboJ. CastanetM-M Marie-Madeleine Loth2006Histological observations of enantiornithine bone [Saurischia, Aves] from the Lower Cretaceous of Las Hoyas [Spain].Comptes Rendus Palevol5685691
- 12. Bakker RT (1986) The Dinosaur Heresies: New Theories Unlocking the Mystery of the Dinosaurs and Their Extinction. New York: Kennsington Publishing Corporation. 481 p.RT Bakker1986The Dinosaur Heresies: New Theories Unlocking the Mystery of the Dinosaurs and Their Extinction.New YorkKennsington Publishing Corporation481
- 13. Ruben J (1991) Reptilian physiology and the flight capacity of Archaeopteryx. Evolution 45: 1–17.J. Ruben1991Reptilian physiology and the flight capacity of Archaeopteryx.Evolution45117
- 14. Chinsamy A, Hillenius WJ (2004) Physiology of non-avian dinosaurs. In: Weishampel DB, Dodson P, Osmólska H, editors. The Dinosauria. 2nd Edition. Berkeley: University of California Press. pp. 643–659.A. ChinsamyWJ Hillenius2004Physiology of non-avian dinosaurs.DB WeishampelP. DodsonH. Osmólska The Dinosauria. 2nd EditionBerkeleyUniversity of California Press643659
- 15. Padian K, Horner JR (2004) Dinosaur physiology. In: Weishampel DB, Dodson P, Osmólska H, editors. The Dinosauria. 2nd Edition. Berkeley: University of California Press. pp. 660–671.K. PadianJR Horner2004Dinosaur physiology.DB WeishampelP. DodsonH. Osmólska The Dinosauria. 2nd EditionBerkeleyUniversity of California Press660671
- 16. Wellnhofer P (2008) Archaeopteryx: Der Urvogel von Solnhofen. Munich: Verlag Dr Friedrich Pfeil. 256 p.P. Wellnhofer2008Archaeopteryx: Der Urvogel von Solnhofen.MunichVerlag Dr Friedrich Pfeil256
- 17. Enlow DH (1969) The bone of reptiles. In: Gans C, d'A Bellairs A, editors. Biology of the Reptilia: Morphology A. London: Academic Press. pp. 45–77.DH Enlow1969The bone of reptiles.C. GansA. d'A Bellairs Biology of the Reptilia: Morphology ALondonAcademic Press4577
- 18. Zhou Z, Zhang F (2002) A long-tailed, seed-eating bird from the Early Cretaceous of China. Nature 418: 405–409.Z. ZhouF. Zhang2002A long-tailed, seed-eating bird from the Early Cretaceous of China.Nature418405409
- 19. Zhou Z, Zhang F (2003) Anatomy of the primitive bird Sapeornis chaoyangensis from the Early Cretaceous of Liaoning, China. Can J Earth Sci 40: 731–747.Z. ZhouF. Zhang2003Anatomy of the primitive bird Sapeornis chaoyangensis from the Early Cretaceous of Liaoning, China.Can J Earth Sci40731747
- 20. Chinsamy A, Elzanowski A (2001) Evolution of growth pattern in birds. Nature 412: 402–403.A. ChinsamyA. Elzanowski2001Evolution of growth pattern in birds.Nature412402403
- 21. Makovicky PJ, Apesteguía S, Agnolín FL (2005) The earliest dromaeosaurid theropod from South America. Nature 437: 1007–1011.PJ MakovickyS. ApesteguíaFL Agnolín2005The earliest dromaeosaurid theropod from South America.Nature43710071011
- 22. Turner AH, Pol D, Clarke JA, Erickson GM, Norell M (2007) A basal dromaeosaurid and size evolution preceding avian flight. Science 317: 1378–1381.AH TurnerD. PolJA ClarkeGM EricksonM. Norell2007A basal dromaeosaurid and size evolution preceding avian flight.Science31713781381
- 23. Padian K, Horner JR, Ricqlés A de (2003) Dinosaurian growth rates and bird origins. Nature 412: 405–408.K. PadianJR HornerA de Ricqlés2003Dinosaurian growth rates and bird origins.Nature412405408
- 24. Clarke JA, Zhou Z, Zhang F (2006) Insight into the evolution of avian flight from a new clade of Early Cretaceous ornithurines from China and the morphology of Yixianornis grabaui. J Anat 208: 287–308.JA ClarkeZ. ZhouF. Zhang2006Insight into the evolution of avian flight from a new clade of Early Cretaceous ornithurines from China and the morphology of Yixianornis grabaui.J Anat208287308
- 25. Foote JS (1916) A contribution to the comparative histology of the femur. Smithson Contr Knowl 35: 1–241.JS Foote1916A contribution to the comparative histology of the femur.Smithson Contr Knowl351241
- 26. Enlow DH, Brown SO (1956) A comparative histological study of fossil and recent bone tissues. Part 1. Tex J Sci 8: 405–443.DH EnlowSO Brown1956A comparative histological study of fossil and recent bone tissues. Part 1.Tex J Sci8405443
- 27. Enlow DH, Brown SO (1957) A comparative histological study of fossil and recent bone tissues. Part 2. Tex J Sci 9: 186–214.DH EnlowSO Brown1957A comparative histological study of fossil and recent bone tissues. Part 2.Tex J Sci9186214
- 28. Enlow DH, Brown SO (1958) A comparative histological study of fossil and recent bone tissues. Part 3. Tex J Sci 10: 187–230.DH EnlowSO Brown1958A comparative histological study of fossil and recent bone tissues. Part 3.Tex J Sci10187230
- 29. Klevezal GA (1996) Recording Structures of Mammals. Rotterdam: AA Balkema. 448 p.GA Klevezal1996Recording Structures of Mammals.RotterdamAA Balkema448
- 30. Sander PM, Tückmantel C (2003) Bone lamina thickness, bone apposition rates, and age estimates in sauropod humeri and femora. Palaeontol Z 77: 161–172.PM SanderC. Tückmantel2003Bone lamina thickness, bone apposition rates, and age estimates in sauropod humeri and femora.Palaeontol Z77161172
- 31. Erickson GM, Makovicky PJ, Inouye BD, Chang-Fu Z, Ke-Qin G (2009) A life table for Psittacosaurus lujiatunensis: Initial insights into ornithischian population biology. Anat Rec 292: 1514–1521.GM EricksonPJ MakovickyBD InouyeZ. Chang-FuG. Ke-Qin2009A life table for Psittacosaurus lujiatunensis: Initial insights into ornithischian population biology.Anat Rec29215141521
- 32. Case TJ (1978) On the evolution and adaptive significance of postnatal growth rates in the terrestrial vertebrates. Quart Rev Biol 53: 243–282.TJ Case1978On the evolution and adaptive significance of postnatal growth rates in the terrestrial vertebrates.Quart Rev Biol53243282
- 33. Houck MA, Gauthier JA, Strauss RE (1990) Allometric scaling in the earliest fossil bird, Archaeopteryx lithographica. Science 247: 195–198.MA HouckJA GauthierRE Strauss1990Allometric scaling in the earliest fossil bird, Archaeopteryx lithographica.Science247195198
- 34. Chiappe LM (1995) The first 85 million years of avian evolution. Nature 378: 349–355.LM Chiappe1995The first 85 million years of avian evolution.Nature378349355
- 35. Chiappe LM, Marugán-Lobón J, Shu'an J, Zhou Z (2008) Life history of a basal bird: morphometrics of the Early Cretaceous Confuciusornis. Biol Lett 4: 719–723.LM ChiappeJ. Marugán-LobónJ. Shu'anZ. Zhou2008Life history of a basal bird: morphometrics of the Early Cretaceous Confuciusornis.Biol Lett4719723
- 36. Ricklefs RE (1973) Patterns of growth in birds II. Growth rate and mode of development. Ibis 115: 177–201.RE Ricklefs1973Patterns of growth in birds II. Growth rate and mode of development.Ibis115177201
- 37. Wells JW (1963) Coral growth and geochronometry. Nature 197: 948–950.JW Wells1963Coral growth and geochronometry.Nature197948950
- 38. Padian K, Chiappe LM (1998) The origin and early evolution of birds. Biol Rev 73: 1–42.K. PadianLM Chiappe1998The origin and early evolution of birds.Biol Rev73142
- 39. Yalden DW (1971) The flying ability of Archaeopteryx. Ibis 113: 349–356.DW Yalden1971The flying ability of Archaeopteryx.Ibis113349356
- 40. Chatterjee S, Templin RJ (2003) The flight of Archaeopteryx. Naturwissen 90: 27–32.S. ChatterjeeRJ Templin2003The flight of Archaeopteryx.Naturwissen902732
- 41. Senter P (2006) Scapular orientation in theropods and basal birds, and the origin of flapping flight. Acta Paleontol Pol 51: 305–313.P. Senter2006Scapular orientation in theropods and basal birds, and the origin of flapping flight.Acta Paleontol Pol51305313
- 42. Boarman WI (1999) Common Raven (Corvus corax). Birds of North America 476: 1–32.WI Boarman1999Common Raven (Corvus corax).Birds of North America476132
- 43. Calder WA III (1984) Size, Function, and Life History. Cambridge: Harvard University Press. 431 p.WA Calder III1984Size, Function, and Life History.CambridgeHarvard University Press431
- 44. Erickson GM, Tumanova TA (2001) Growth curve of Psittacosaurus mongoliensis Osborn (Ceratopsia: Psittacosauridae) inferred from long bone histology. Zool J Linn Soc 130: 551–566.GM EricksonTA Tumanova2001Growth curve of Psittacosaurus mongoliensis Osborn (Ceratopsia: Psittacosauridae) inferred from long bone histology.Zool J Linn Soc130551566
- 45. Erickson GM, Curry-Rogers K, Yerby S (2001) Dinosaur growth patterns and rapid avian growth rates. Nature 412: 429–433.GM EricksonK. Curry-RogersS. Yerby2001Dinosaur growth patterns and rapid avian growth rates.Nature412429433
- 46. McLennan JA, Dew L, Miles J, Gillingham N, Waiwai R (2004) Size matters: predation risk and juvenile growth in North Island brown kiwi (Apteryx mantelli). New Zeal J Ecol 28: 241–250.JA McLennanL. DewJ. MilesN. GillinghamR. Waiwai2004Size matters: predation risk and juvenile growth in North Island brown kiwi (Apteryx mantelli).New Zeal J Ecol28241250
- 47. McNab BK (1996) Metabolism and temperature regulation of kiwis (Apterygidae). Auk 113: 687–692.BK McNab1996Metabolism and temperature regulation of kiwis (Apterygidae).Auk113687692
- 48. Senter P, Robins JH (2003) Taxonomic status of the specimens of Archaeopteryx. J Vert Paleo 23: 961–965.P. SenterJH Robins2003Taxonomic status of the specimens of Archaeopteryx.J Vert Paleo23961965
- 49. Erickson GM, Makovicky PJ, Norell MA, Yerby SA, Brochu CA (2004) Gigantism and comparative life history parameters of tyrannosaurid dinosaurs. Nature 430: 772–775.GM EricksonPJ MakovickyMA NorellSA YerbyCA Brochu2004Gigantism and comparative life history parameters of tyrannosaurid dinosaurs.Nature430772775
- 50. Francillon-Vielliot H, Buffrénil de V, Castanet J, Géraudie J, Meunier FJ, et al. (1990) Microstructure and mineralization of vertebrate skeletal tissues. In: Carter JG, editor. Biomineralization: Patterns and Evolutionary Trends. New York: Van Nostrand Reinhold. pp. 471–530.H. Francillon-VielliotV. Buffrénil deJ. CastanetJ. GéraudieFJ Meunier1990Microstructure and mineralization of vertebrate skeletal tissues.JG CarterBiomineralization: Patterns and Evolutionary TrendsNew YorkVan Nostrand Reinhold471530
- 51. Goloboff PA, Farris JS, Nixon K (2003) TNT: tree analysis using new technologies. PA GoloboffJS FarrisK. Nixon2003TNT: tree analysis using new technologies.Program and documentation available from the authors and at http://www.zmuc.dk/public/phylogeny. Program and documentation available from the authors and at http://www.zmuc.dk/public/phylogeny.
- 52. Castanet J, Grandin A, Abourachid A, Ricqlés A de (1996) Expression de la dynamique de croissance dans la structure de l'os périostique chez Anas platyrhynchos. C R Acad Sci Paris, Sciences de la vie 319: 301–308.J. CastanetA. GrandinA. AbourachidA de Ricqlés1996Expression de la dynamique de croissance dans la structure de l'os périostique chez Anas platyrhynchos.C R Acad Sci Paris, Sciences de la vie319301308
- 53. Castanet J, Curry Rogers K, Cubo J, Boisard J-J (2000) Periosteal bone growth rates in extant ratites (ostriche and emu). Implications for assessing growth in dinosaurs. C R Acad Sci Paris, Sciences de la vie 323: 543–550.J. CastanetK. Curry RogersJ. CuboJ-J Boisard2000Periosteal bone growth rates in extant ratites (ostriche and emu). Implications for assessing growth in dinosaurs.C R Acad Sci Paris, Sciences de la vie323543550
- 54. Alexander R McN (1983) Allometry of the leg bones of moas (Dinornithes) and other birds. J Zool Lond 200: 215–231.R McN Alexander1983Allometry of the leg bones of moas (Dinornithes) and other birds.J Zool Lond200215231
- 55. Blueweiss L, Fox H, Kudzma V, Nakashima D, Peters R, Sams S (1978) Relationships between body size and some life history parameters. Oecologica 37: 257–272.L. BlueweissH. FoxV. KudzmaD. NakashimaR. PetersS. Sams1978Relationships between body size and some life history parameters.Oecologica37257272
- 56. Lunn DJ, Thomas A, Best N, Spiegelhalter D (2000) WinBUGS – a Bayesian modelling framework: concepts, structure, and extensibility. Stat and Comput 10: 325–337.DJ LunnA. ThomasN. BestD. Spiegelhalter2000WinBUGS – a Bayesian modelling framework: concepts, structure, and extensibility.Stat and Comput10325337