In eukaryotes, the spindle assembly checkpoint (SAC) ensures that chromosomes undergoing mitosis do not segregate until they are properly attached to the microtubules of the spindle.
We investigated the mechanism underlying this surveillance mechanism in plants, by characterising the orthogolous SAC proteins BUBR1, BUB3 and MAD2 from Arabidopsis. We showed that the cell cycle-regulated BUBR1, BUB3.1 and MAD2 proteins interacted physically with each other. Furthermore, BUBR1 and MAD2 interacted specifically at chromocenters. Following SAC activation by global defects in spindle assembly, these three interacting partners localised to unattached kinetochores. In addition, in cases of ‘wait anaphase’, plant SAC proteins were associated with both kinetochores and kinetochore microtubules. Unexpectedly, BUB3.1 was also found in the phragmoplast midline during the final step of cell division in plants.
We conclude that plant BUBR1, BUB3.1 and MAD2 proteins may have the SAC protein functions conserved from yeast to humans. The association of BUB3.1 with both unattached kinetochore and phragmoplast suggests that in plant, BUB3.1 may have other roles beyond the spindle assembly checkpoint itself. Finally, this study of the SAC dynamics pinpoints uncharacterised roles of this surveillance mechanism in plant cell division.
Citation: Caillaud M-C, Paganelli L, Lecomte P, Deslandes L, Quentin M, Pecrix Y, et al. (2009) Spindle Assembly Checkpoint Protein Dynamics Reveal Conserved and Unsuspected Roles in Plant Cell Division. PLoS ONE 4(8): e6757. doi:10.1371/journal.pone.0006757
Editor: Michael Freitag, Oregon State University, United States of America
Received: May 19, 2009; Accepted: July 27, 2009; Published: August 27, 2009
Copyright: © 2009 Caillaud et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the Agence Nationale de la Recherche ANR-08-GENM-014 SCRIPS grant. M.-C. C. and L.P. were supported by fellowships from the French Ministere de la Recherche et de l'Enseignement Superieur. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
In eukaryotes, the spindle assembly checkpoint (SAC) is a sophisticated surveillance mechanism that ensures the fidelity of chromosome segregation during mitosis , . The SAC monitors the interaction between chromosomes and microtubules (MTs) at specialised chromosomal regions, the kinetochores. In response to unattached kinetochores and to kinetochores lacking tension, the SAC is activated and localised to unattached kinetochores. The SAC transmits a “wait anaphase” signal until all chromosomes achieve bipolar attachment. This signal is transmitted through the inhibition of anaphase-promoting complex/cyclosome (APC/C) activity by sequestration of the CDC20 cofactor. SAC components were first identified through genetic screens in budding yeast and include the MAD (mitotic arrest-deficient) and BUB (budding uninhibited by benzymidazol) proteins , . In metazoans and yeast, the mitotic checkpoint complex (MCC), which contains the three SAC proteins MAD2, MAD3 (equivalent of BUBR1, for BUB1-related, in higher eukaryotes) and BUB3 together with CDC20, is regarded as the SAC effector –. In budding yeast, the SAC is a non-essential device and it only becomes essential in response to ‘damage’ that is perturbations in the kinetochore-MT attachment process , . On the other hand, in metazoans, the SAC is an essential pathway, the integrity of which is required to prevent chromosome mis-segregation and cell death . In plants, SAC protein homologs have been identified in silico –, but function has been investigated only for MAD2 for which localisation to unattached kinetochores has been demonstrated by immunolocalisation , .
In this paper, we investigated how this surveillance mechanism operates in the green kingdom. We demonstrated physical interactions between A. thaliana BUBR1, BUB3.1 and MAD2 and their dynamics at unattached kinetochores. In cases of ‘wait anaphase’, plant BUBR1, BUB3.1 and MAD2 proteins were unexpectedly associated with both kinetochores and kinetochore microtubules. Our findings suggest that plant BUBR1, BUB3.1 and MAD2 have both the SAC protein functions conserved from yeast to humans and pinpoints uncharacterised roles in plant cell division.
Results and Discussion
As a first attempt to study SAC during the plant cell cycle, candidate A. thaliana orthologs of the human essential mitotic checkpoint complex proteins BUBR1, BUB3 and MAD2 were identified by OrthoMCL  clustering of orthologous proteins from six model eukaryotic species. The six complete proteomes compared included those of plants (A. thaliana and Oryza sativa), human (Homo sapiens), insect (Drosophila melanogaster) and nematodes (Caenorhabditis elegans and Meloidogyne incognita). A. thaliana BUBR1 (AT2G33560) is a 46 kD protein containing an N-terminal MAD3-BUB1 conserved domain and two KEN boxes conferring substrate recognition by APC/C  (Fig. S1). These two KEN boxes are conserved from yeast MAD3 to human BUBR1 and are required for the concerted action of MAD3 and MAD2 in the checkpoint inhibition of CDC20-APC/C –. Like the MAD3 proteins of Saccharomyces cerevisiae and Schizosaccharomyces pombe, A. thaliana BUBR1 differs from human BUBR1 by the absence of a C-terminal kinase domain. However, the kinase activity of BUBR1 has been shown to be dispensable for spindle checkpoint function in Xenopus larvei . Two A. thaliana BUB3 proteins (BUB3.1, AT3G19590; BUB3.2, AT1G49910) were identified. Both are 38 kD proteins containing WD40 repeats, which have been shown to be involved in the association of BUB3 with MAD2, MAD3 and CDC20 in yeast . A. thaliana BUB3.1 and BUB3.2 are 88% identical. BUB3.1 is 52% and 22% identical to the human and S. cerevisiae BUB3 proteins , , respectively, over its entire length (Fig. S2). A. thaliana MAD2 (AT3G25980) is a 24 kD protein containing a HORMA domain. It is 44% identical to the human MAD2 protein  and 81% identical to the maize MAD2 protein , over its entire length (Fig. S3).
Arabidopsis BUBR, BUB3.1 and MAD2 genes were expressed in tissues enriched in dividing cells
We investigated the pattern of expression of the A. thaliana BUBR1, BUB3.1, BUB3.2 and MAD2 genes during plant development, using A. thaliana transgenic lines transformed with the corresponding promoter-GUS reporter gene constructs. Similar patterns of GUS expression were observed for the BUBR1, BUB3.1 and MAD2 promoters, both of which directed expression in tissues with a high proportion of dividing cells, early in organ development, in young leaves (Fig. 1A), lateral root primordia (Fig. 1B), lateral root meristems (Fig. 1C) and root meristems (Fig. 1D). Individual cells with strong GUS activity were observed in root meristems. In contrast to the cell cycle regulated pattern observed for both BUBR1, BUB3.1 and MAD2 promoter, no GUS activity in dividing cells was observed for the BUB3.2 promoter in young leaves (Fig. 1A), lateral root primordia (Fig. 1B), lateral root meristems (Fig. 1C) and root meristems (Fig. 1D). These results are consistent with global transcriptome and RT-PCR analysis showing that BUB3.1, BUBR1 and MAD2 presented a distinct expression peak at the G2/M boundary in synchronised A. thaliana cell cultures that was not observed for BUB3.2 , . Because BUB3.2 was not a cell cycle regulated gene, we next focused on BUB3.1 candidate gene.
Promoter:GUS fusions revealed BUBR1, BUB3.1 and MAD2 expression in the developing leaves of 7-day-old seedlings (A), in lateral root primordia (B), lateral root meristems (C) and root meristems (D). BUB3.2 expression was only detected in cotyledons (insert). Bars, 200 µm (A), 50 µm (B), 100 µm (C and D).
BUBR1 and MAD2 interact specifically at chromocenters
In yeast and humans, BUBR1, BUB3 and MAD2 may be found together in large complexes (mitotic checkpoint complex) , , . To carry out possible interactions between the cell cycle-regulated A. thaliana BUBR1, BUB3.1 and MAD2, a yeast two-hybrid split-ubiquitin approach was used. It is based on the fusion of the prey and the bait to the N- and C-terminal halves of ubiquitin (Nub and Cub, respectively), which are then able to form a native-like ubiquitin upon interaction . Ubiquitin-specific proteases recognize the reconstituted ubiquitin and cleave off a reporter protein, URA3, linked to the C terminus of Cub and whose degradation results in uracil auxotrophy and 5-FOA resistance. Coexpression of BUBR1:Cub:URA3 with either Nub:BUB3.1 and Nub:MAD2 conferred resistance to 5-FOA, indicating that BUBR1 interacted with both BUB3.1 and MAD2. BUB3.1 and MAD2 also interacted (Fig. 2A). These interactions were confirmed in a reciprocal bait-prey experiment.
(A) Interactions in the yeast two-hybrid split-ubiquitin system. Dilution series of yeast JD53 cells expressing both bait fusions (BUBR1 or MAD2:Cub:URA3) and prey fusions (Nub:BUB3.1 or MAD2) were grown on yeast medium minus histidine and tryptophan (-HW) but containing 5-FOA, as indicated. Interaction resulted in uracil auxotrophy and 5-FOA resistance. (B) Single-plane images of tobacco epidermal leaf cells infiltrated with A. tumefaciens expressing BUBR1:GFP, BUB3.1:GFP or MAD2:GFP constructs. (C) Principle of in vivo bimolecular fluorescence complementation (BiFC). BiFC is based on the fusion of the prey (a) and bait (b) to the N- and C-terminal halves of the yellow fluorescent protein YFP (YN and YC, respectively), forming a functional YFP upon interaction . (D–G) In planta BiFC assay. Single-plane confocal images of epidermal leaf cells infiltrated with A. tumefaciens co-expressing (D) BUBR1-YC and BUB3.1-YN or BUB3.1-YC and MAD2-YN, (E) BUB3.1-YC and MAD2-YN (green channel) and BUBR1-GFP (red channel), (F) BUBR1-YC and MAD2-YN, (G) BUBR1-YC and MAD2-YN (green channel) and GFP-HTR12 (red channel) fusion constructs. The merged images show (E) nuclear BUBR1, BUB3.1 and MAD2 colocalisation in yellow, (F–G) that BUBR1 and MAD2 interaction colocalised with (F) bright chromocenter spots stained with DAPI (blue channel) and with (G) the centromeric marker GFP-HTR12. n, nucleus. Bars, 25 µm (B, D and E), 5 µm (F and G).
To better characterise the physical interactions between BUBR1, BUB3.1 and MAD2, we performed in planta localisation of these interactions. Following transient expression of the appropriate gene construct in Nicotiana benthamiana leaf epidermis, BUBR1 fused to GFP (BUBR1:GFP) was specifically targeted to the nucleus (n = 30; Fig. 2B), whereas BUB3.1:GFP and MAD2:GFP were detected in both the nucleus and the cytoplasm (n = 30; Fig. 2B). Using bimolecular fluorescence complementation (BiFC; , we demonstrated a close interaction between BUBR1, BUB3.1 and MAD2. Coexpression of the constructs encoding BUBR1:YC (BUBR1 fused to the C-terminal half of YFP) and BUB3.1:YN (BUB3 fused to the N-terminal half of YFP) resulted in the reconstituted YFP complexes only in the nuclei (n = 20; Fig. 2C–D). In addition, BUB3.1 interacted with MAD2 in the nuclei and cytoplasm of epidermal cells (n = 20; Fig. 2D). No YFP fluorescence was detected in negative control experiments in which BUBR1:YN, BUB3.1:YN, BUB3.1:YC or MAD2:YC was produced together with the corresponding vector control (n = 30). Coexpression of the constructs encoding BUB3.1:YC, MAD2:YN, and BUBR1:GFP showed that BUB3.1 and MAD2 interact, and that they co-localise with BUBR1 in the nucleus (n = 20; Fig. 2E). Interactions between BUBR1:YC and MAD2:YN were observed exclusively in the nucleus, as bright subnuclear foci (n = 40; Fig. 2F). Within the nuclei, fluorescence signals were localised with the core of bright DAPI-stained condensed chromocenters (Fig. 2F). Using the centromeric Histone H3 variant from A. thaliana GFP:HTR12 (CENH3, AT1G01370) as in vivo marker for centromeres –, we confirmed that BUBR1 and MAD2 interact at interphase centromeres (n = 10; Fig. 2G) corresponding to the position on the chromosome at which kinetochore proteins associate.
BUBR1, BUB3.1 and MAD2 localised to the kinetochores following SAC activation
In metazoan cells, the BUBR1, BUB3 and MAD2 proteins are specifically localised to the kinetochores following the activation of the SAC by global defects in spindle assembly in cells treated with microtubule poisons , –. The maize and wheat MAD2 proteins are the only plant SAC proteins for which localisation to unattached kinetochores has been demonstrated , . By combined direct immunofluorescence of maize MAD2 and CENPC, the identity of the MAD2-positive regions as kinetochores has been demonstrated .
To gain insight into the spindle checkpoint activation in plant, we profiled the spatial distribution of A. thaliana SAC proteins in tobacco cell cultures stably expressing the BUBR1:GFP, BUB3.1:GFP and MAD2:GFP constructs. At a prometaphase-like stage, following treatment with the microtubule-destabilizing herbicide propyzamid, which prevents the formation of microtubule-kinetochore attachments, the MAD2 fusion protein was found to cluster strongly in bright spots on condensing chromosomes corresponding to unattached kinetochores (n = 20; Fig. 3). Similar localisation was observed for the BUB3.1 and BUBR1 fusion proteins (n = 20; Fig. 3). Thus, the plant BUBR1, BUB3.1 and MAD2 partners identified in this study are all in place at the unattached kinetochores and may therefore fulfil the evolutionarily conserved functions of SAC proteins, delaying anaphase until all the chromosomes are attached to both poles of the spindle.
Single optical section of prometaphase-like arrested cells expressing BUBR1:GFP, BUB3.1:GFP and MAD2:GFP fusion constructs, 1 h after propyzamid treatment. In merged images, the yellow colour corresponds to BUBR1:GFP, BUB3.1:GFP or MAD2:GFP (green channel) colocalisation with SYTO 82 (red channel). BUBR1:GFP, BUB3.1:GFP and MAD2:GFP localise in vivo to the kinetochores of chromosomes stained with SYTO 82 orange-fluorescent nucleic acid stain. Bars, 5 µm.
We further analysed plant SAC protein distribution in living cells in cases of delayed anaphase onset. As anaphase initiation requires the ubiquitylation and degradation by the 26S proteasome of key mitotic regulators , such as the separase inhibitor securin and the Cdk1 subunit cyclin B, we studied cells that had been treated with the proteasome inhibitor MG132. The MG132 tripeptide has been shown to be a very efficient proteasome inhibitor in mammalian and plant cell cultures. It preserves metaphase spindles and kinetochore-microtubule (kMT) attachments but inhibits the onset of anaphase . As previously reported in plants , , two hours after the addition of this molecule to a concentration of 100 µM, tobacco cells arrested in metaphase were found to have highly condensed chromosomes (n = 30; Fig. 4A). At this time point, A. thaliana BUBR1, BUB3.1 and MAD2 were localised to the sister kinetochores of condensed chromosomes in metaphase arrested cells (n = 20; Fig. 4A). In cells in which chromosomes were aligned at the spindle equator, BUBR1, BUB3.1 and MAD2 were found to be present in all the kinetochores (n = 15; Fig. 4B). Progressively, much of the BUBR1, BUB3.1, MAD2:GFP-derived fluorescence took on a fibrillar appearance, probably as a result of association with the acentrosomal metaphase spindle apparatus (n = 30; Fig. 4B). Three hours after MG132 treatment, the initially diffuse spindle BUBR1, BUB3.1 and MAD2:GFP staining accumulated progressively onto MT-like structures within the spindle (n = 25; Fig. 4C). At this time point, bright spots corresponding to kinetochores were also detected for BUBR1, BUB3.1 and MAD2 (n = 25; Fig 4C). To determine if the MT-like SAC fluorescence was in fact MT dependent, we treated BUBR1:GFP cells with the MT-stabilizing agents Paclitaxel. Three hours after MG132 treatment, the adjunction of Paclitaxel dramatically intensified the fibrillar nature of BUBR1:GFP (n>20; Fig. S4). In addition, immunostaining of β-tubulin confirmed that BUBR1 colocalized with spindle MTs when proteolysis is blocked by MG132 (n>10; Fig. S4). Previous reports have provided evidence for the motor-assisted transport of human MAD2 complexes from kinetochores to the spindle poles along MTs . This mechanism may play an important role in removing checkpoint proteins from the kinetochores and turning off the checkpoint. Based on our observations, plant SAC proteins have an intriguing intracellular distribution, apparently accumulating onto both kinetochores and the spindle MTs in cell arrested in metaphase.
Single optical section of cells expressing BUBR1:GFP, BUB3.1:GFP and MAD2:GFP fusion constructs (green channel) after treatment with 100 µM MG132. Chromosomes in living cells were stained with SYTO 82 (red channel). In merged images, the yellow colour corresponds to the colocalisation of BUBR1:GFP, BUB3.1:GFP or MAD2:GFP with SYTO 82. (A) Two hours after MG132 treatment, BUBR1, BUB3.1 and MAD2 were localised into two bright spots per condensed chromosome, corresponding to kinetochores. (B) When chromosomes were arrested in metaphase, BUBR1, BUB3.1 and MAD2 localised to all the kinetochores of chromosomes arrested in metaphase. A diffuse signal in the metaphase spindle apparatus was also observed for BUBR1, BUB3.1 and MAD2. (C) Three hours after MG132 treatment, BUBR1, BUB3.1 and MAD2 localised to bright spots corresponding to the kinetochores of chromosomes and staining accumulated onto MT-like structures within the spindle in metaphase arrested cells. Bars, 2 µm (A), 5 µm (B and C).
SAC inactivation in normal cell division
We then investigated the distribution of A. thaliana SAC proteins in vivo in normal mitosis conditions, when SAC is inactivated. This was made possible since the expression of the chimeric proteins did not prevent cell cycle progression. In the absence of SAC activation, BUBR1 was found exclusively in nuclei stained with SYTO 82 during interphase (n = 30; Fig. 5A). BUB3.1 and MAD2 proteins were localised to the nucleus and gave a weak cytoplasmic signal during interphase (n = 30; Fig. 5A). In early prophase, BUBR1, BUB3.1 and MAD2 became localised in the cytoplasm following nuclear envelope breakdown and remained there until the end of metaphase (n = 10; Fig. 5A). By telophase, when a new nuclear envelope forms around each set of separated sister chromosomes, A. thaliana BUBR1 and MAD2 were again concentrated in the nucleus (n = 15; Fig. 5A).
(A) Single optical section of cells expressing BUBR1:GFP, BUB3.1:GFP and MAD2:GFP fusion constructs (green channel). Chromosomes in living cells were stained with SYTO 82 (red channel). In merged images, the yellow colour corresponds to the colocalisation of BUBR1:GFP, BUB3.1:GFP or MAD2:GFP with SYTO 82. By telophase, BUB3.1:GFP was detected in daughter nuclei (n) and in the midline at the cell periphery (arrow), forming a ring around the edge of the newly formed cell plate. (B) Selected frames from a fluorescence time-lapse analysis of the distribution of BUB3.1:GFP during cytokinesis. Single optical section of a cell expressing the BUB3.1:GFP fusion construct (green channel). After chromosome separation, BUB3.1 is localised along the midline of the anaphase spindle (arrowhead). During telophase, BUB3.1 is gradually transferred into the daughter nuclei. During phragmoplast extension from the centre to the periphery of the cell, BUB3.1 localises with the margin of the expanded phragmoplast. At the end of telophase, BUB3.1 is present at the cell periphery, forming a ring around the edge of the newly formed cell plate. This specific localisation at the phragmoplast midline disappeared when the newly formed cell plate completely separated the two daughter cells. At the end of cytokinesis, BUB3.1 was again concentrated in the nucleus. Time is in min:s. Bars, 10 µm.
Overall, our data show that checkpoint proteins are only recruited at kinetochore in case of damage in spindle assembly. During normal mitosis, BUBR1, BUB3.1 and MAD2 staining at the kinetochore was not detected, inconsistent with reports for metazoan cells , , . We propose that organism-specific differences in the behaviour of SAC are likely to reflect evolutionary divergence in the mechanics of spindle assembly rather than extensive differences in the pathways of checkpoint signalling. Animal cells undergo an open mitosis in which prometaphase chromosomes are initially free of spindle microtubules after nuclear envelope breakdown. High levels of MAD and BUB proteins are present on these unattached kinetochores , , . Plant cells undergo mitosis in which acentrosomal pro-spindle assembly is initiated before nuclear envelope breakdown . Our data suggest that in plant, kinetochores do not recruit high level of these SAC proteins during normal mitosis which is consistent with the idea that plant chromosomes are continuously linked to MTs.
BUB3.1 localised to phragmoplast midline during plant cytokinesis
BUB3.1 displayed an unexpected distribution during cytokinesis in late anaphase to telophase in plant cells. It first appeared in the centre of the forming cell plate, and was subsequently redistributed to the growing margins of the cell plate as the cell plate grew outwards. Time-lapse analysis showed A. thaliana BUB3.1 was strongly localised to the anaphase spindle midline after chromosome separation in late anaphase (n = 15; Fig. 5B). During the final stages of cell division, a cytokinetic apparatus unique to plants, the phragmoplast, was generated. The phragmoplast directs Golgi-derived vesicles to the midline where they fuse to form a cell plate, permitting the separation of the daughter cells. During telophase, a strong BUB3.1:GFP signal was detected in the early phragmoplast midline and in the newly formed daughter nuclei (n = 17; Fig. 5B). At the end of telophase, when the phragmoplast was fully expanded, BUB3.1 was observed at the cell periphery, forming a ring around the edge of the newly formed cell plate (n = 20; Fig. 5A–B). This signal disappeared when the fully expanded cell plate completely separated the two daughter cells (n = 18; Fig. 5B). This BUB3.1 subcellular localisation appeared intriguing since metazoan and yeast BUB3 has not been described to be involved in cytokinesis. In animal cells, after sister chromosomes have separated, the remaining non-kinetochore MTs form a structure called the spindle midzone. The spindle midzone was compressed by the ingressing cleavage furrow. This spindle remnant also persists during cytokinesis in plant cells, where it becomes the early phragmoplast. The difference is that, instead of being the focus of constriction, as in animals, the central spindle/early phragmoplast opens out as a ring that directs Golgi-derived vesicles to the midline where they fuse to form a cell plate.
We found that, during outward cytokinesis, BUB3.1 was specifically localised to the phragmoplast midline, at which the cell plate was held by phragmoplast MTs. The BUBR1 and MAD2 proteins did not follow this pattern. Thus, BUB3.1, in addition to its known role in the spindle assembly checkpoint itself, may have a plant-specific role in late mitosis coordinating phragmoplast expansion. The phragmoplast midline defines the interface between phragmoplast MT plus-ends and the newly formed cell plate. Recent studies indicated that the phragmoplast midline could contain linker molecules that help to stabilize MT plus-ends and connect them to cell plate membranes. This results in optimally organized phragmoplast MTs that deliver the Golgi-derived vesicles to the growing cell plate .
We hypothesize, that BUB3.1 could be part of a MT plus-end capture complex associated with other “phragmoplast midline proteins” and then may regulate phragmoplast expansion, essential for cytokinesis. An analysis of the cell cycle regulators present in synchronised A. thaliana cell cultures showed that BUB3.1 expression was coregulated with the expression of other cytokinesis-related genes . In addition, the AURORA-like kinase 1 , the microtubule-associated protein MAP65-3 , the molecular motor kinesin PAKRP2  and the CDC27/HOBBIT APC/C subunit  have phragmoplast midline distributions similar to that of BUB3.1 during cytokinesis. However, none of these proteins has been reported to be localised to both unattached kinetochores and the phragmoplast midline. The association of BUB3.1 with both these structures suggests that plants may coordinate spindle assembly and cytokinesis through shared machinery. This study provides clues to the possible functional links between the spindle and phragmoplast assembly checkpoints, ensuring failsafe mitosis.
Materials and Methods
Sequence identification and gene cloning
A. thaliana proteins orthologous to human BUB3, BUBR1 and MAD2 were identified by the OrthoMCL  clustering of six proteomes based on standard parameters. The six proteomes compared were those of A. thaliana (TAIR, http://www.arabidopsis.org), Homo sapiens (http://www.ncbi.nlm.nih.gov/projects/CCDS/CcdsBrowse.cgi), Oryza sativa (http://rice.plantbiology.msu.edu/), Drosophila melanogaster (http://flybase.org/), Caenorhabditis elegans (http://wormbase.org/) and Meloidogyne incognita (http://meloidogyne.toulouse.inra.fr/). Interpro scans (http://www.ebi.ac.uk/interpro) were used to study domain organisation. The A. thaliana BUBR1, BUB3.1, MAD2 and HTR12/CENP-A coding sequences were amplified by PCR, using specific primers (Fig. S5). They were inserted into the pDON207 donor vector and then into the pK7FWG2, or pK7WGF2 for HTR12, plant expression vector and BiFC vectors (pAM-35SS-GWY-YFPc and pAM-35SS-GWY-YFPn), using Gateway Technology (Invitrogen).
Promoter analysis and histochemical localisation of GUS activity
For the promoter:GUS fusion, fragments of the 1365 bp, 1001 bp, 999 bp and 1000 bp immediately upstream from the start codon, for BUB3.1, BUB3.2, MAD2 and BUBR1, respectively, were amplified by PCR (Fig. S5), inserted into the pDON207 donor vector and then into the pKGWFS7 plant vector, using Gateway Technology (Invitrogen). Wild-type (WS ecotype) A. thaliana plants were stably transformed and GUS activity was assayed histochemically, as previously described , on 10 independent transformed plants for each construct. Samples were observed with a Zeiss Axioplan 2 microscope and images analysed with AxioVision 4.7 (Zeiss).
Yeast two-hybrid split-ubiquitin assay
The split-ubiquitin assay was carried out in S. cerevisiae strain JD53, as previously described . The BUBR1, BUB3.1 and MAD2 coding sequences were inserted into the GW:Cub:URA3 bait vector (pMKZ) and the NuI:GW prey vector, using the Gateway system. Standard procedures were used for yeast growth and transformation. Transformants were selected on 5-fluoroorotic acid (5-FOA) plates containing minimal medium with yeast nitrogen base without amino acids (Difco) and glucose, supplemented with lysine, leucine, uracil (M-HW), and 1 mg/ml 5-FOA.
N. benthamiana transformation and cell cultures
N. benthamiana plants were grown under continuous light for 1 month at 26°C. Infiltration of Agrobacterium tumefaciens into tobacco leaves was as described  and plants were analysed two days after infiltration. For tobacco cell culture establishment, N. benthamiana leaves were cocultured two days with A. tumefaciens in the dark at 26°C, rinsed in a liquid MS medium containing 3% sucrose and 150 mg/l cefotaxime (Sigma). The tissue was blotted dry and placed on regeneration medium (MS medium, 3% sucrose, 1.0 mg/l indole acetic acid, and 0.1 mg/l benzyladenine, Sigma, 0.8% agar), and supplemented with 150 mg/l cefotaxime and 50 mg/l kanamycin. Explants were incubated in a controlled growth chamber at 26°C. All explants were subcultured onto fresh regeneration/selection medium every 10 days. Two explants were used to generate suspension cultures: stably transformed explants were placed on MS medium supplemented with 0.5 mg/l 2,4D (2,4-dichlorophenoxyacetic acid) and 40 mg/l kanamycin for the induction of callus, which was transferred into liquid MS medium supplemented with 1 mg/l 2,4D and 50 mg/l kanamycin. The cultures were incubated at 26°C in the dark with continuous shaking.
Drug treatments and microscopy
Optical sections of tobacco leaf epidermal cells or tobacco cell cultures were observed with a ×63 water immersion apochromatic objective (numerical aperture 1.2, Zeiss) fitted to an inverted confocal microscope (Axiovert 200 M, LSM510 META; Zeiss) at 25°C. GFP and SYTO 82 (Molecular Probes) fluorescence were monitored in Channel mode with a BP 505–530, 488 beam splitters and LP 530 filters for GFP and 545 nm beam splitters for SYTO 82 (488 nm excitation line). For DAPI staining, cells were first fixed in 1×PBS+2% paraformaldehyde in PBS (1 x) supplemented with 0.05% Triton X-100. DNA was stained in vivo with the orange fluorescent dye SYTO 82 (2 µM final concentration). Propyzamid (Sigma), Paclitaxel (Sigma) and carbobenzoxyl-leucinyl-leucinyl-leucinal (MG132; kindly provided by M. C. Criqui, IBMP, Strasbourg, France) were used at final concentrations of 50 µM, 50 µM and 100 µM, respectively. These preparations were stored for no more than one month at −20°C. The samples treated with MG132 were collected at different time point to be observed during metaphase arrest by in vivo confocal microscopy. For Propyzamid and Paclitaxel treatments, samples were collected 10 min after drug adjunction and used immediately for observation. Digital images were analysed using LSM Image Browser (Zeiss), imported to Photoshop CS2 (Adobe) and contrast/brightness was uniformly changed. For immunolocalization of β-tubulin, samples were collected 3 hours after MG132 treatment. Cells were first fixed in 1×PBS+2% paraformaldehyde supplemented with 0.05% Triton X-100. Immunolabeling was performed according to Ritzenthaler et al.. Cells were incubated overnight with the monoclonal anti-β-tubulin clone TUB 2.1 (Sigma-Aldrich). Two hours incubation at room temperature was performed with Alexa 596 goat antimouse IgG (Molecular Probes, Eugene, OR, USA). DNA was stained with 1 µg.ml−1 4′,6-diamidino-2-phenylindole (DAPI, Sigma) in PBS 1 x buffer. GFP and Alexa 596 (Molecular Probes) fluorescences were monitored in Channel mode with a BP 505–530, HFT 488 beam splitters for GFP and LP 530 filters NFT, 545 nm beam splitters for Alexa Red (488 nm excitation line).
Sequence comparison of BUBR1/MAD3-related proteins. (A) Domain organisation of Arabidopsis thaliana AtBUBR1, Homo sapiens HsBUBR1 and Saccharomyces cerevisiae ScMAD3. Proteins are drawn to scale. (B) Alignment of AtBUBR1 protein from A. thaliana (AtBUBR1, At2g33560) with MAD3 protein from Schizosaccharomyces pombe (SpMAD3, O59767) and the NH2-terminal domains of BUBR1/MAD3 proteins from human (HsBUBR1, O60566), Xenopus larvei (XBUB1B, Q8JGT8) and S. cerevisiae (ScMAD3, P47074). The MAD3-BUB1 domain (PF08311), KEN boxes and BUB3-binding domain of hBUB1B are indicated.
(0.04 MB PDF)
Sequence comparison of BUB3-related proteins. (A) Domain organisation of Arabidopsis thaliana AtBUB3.1 and Homo sapiens HsBUB3. (B) Alignment of the BUB3-related proteins from A. thaliana (AtBUB3.1, At3g19590; AtBUB3.2, At1g49910), Drosophila melanogaster (DmBUB3, NP477381), Homo sapiens (HsBUB3, O43684), Mus musculus (mBUB3, Q9WVA3), Xenopus larvei (XBUB3, Q98UH2) and Saccharomyces cerevisiae (ScBUB3, P26449). The WD-40 repeats are underlined and the BUB3 WD signature sequence indicated by asterisks.
(0.02 MB PDF)
Sequence comparison of MAD2-related proteins. (A) Domain organisation of Arabidopsis thaliana AtMAD2 and human HsMAD2. (B) Alignment of the MAD2-related proteins from A. thaliana (AtMAD2, At3g25980), Zea mays (ZmMAD2, Q9XFH3), mouse (mMAD2, Q5HZH8), human (HsMAD2, AAC50781), Xenopus larvei (XMAD2, AAB41527) and Saccharomyces cerevisiae (ScMAD2, P40958). The HORMA domain (PF02301) is underlined. Identical amino acid residues are coloured.
(0.02 MB PDF)
Subcellular localisation of BUBR1 in MG132-treated tobacco cells. (A) Single optical images of cells expressing BUBR1:GFP fusion construct (green channel) treated with 100 µM MG132 (3 h), and then with 50 µM paclitaxel (10 min). Chromosomes in living cells were stained with SYTO 82 (red channel). The adjunction of Paclitaxel dramatically intensified the spindle MT-like structures of BUBR1:GFP. (B) Co-visualisation of MT spindle apparatus and BUBR1, three hours after 100 µM MG132 treatments. In merged image, the yellow colour corresponds to BUBR1:GFP (green channel) colocalisation with β-tubulin immunostaining (red channel). Bars, 5 µm.
(0.28 MB PDF)
Gateway primers used in this study.
(0.01 MB PDF)
We thank Marylin Vantard (iRTSV, Grenoble, France), Pascal Genschik, Laurent Pieuchot, Anne Catherine Schmit (IBMP, Strasbourg, France), and Michel Ponchet (IBSV, France) for helpful discussions, Etienne G.J. Danchin for OrthoMCL clustering, Catherine Mura for growing tobacco plants, Mansour Karimi (VIB Ghent, Belgium) for the plant Gateway vectors, Imre E. Somssich (Max-Planck Institut, Köln, Germany) for the split-ubiquitin system, Marie-Claire Criqui (IBMP, France) for MG132 inhibitor and helpful discussions. This paper is dedicated to the just born Elyas Marfaing.
Conceived and designed the experiments: MCC LP PL LD PA BF. Performed the experiments: MCC LP PL MQ YP. Analyzed the data: MCC LP PL MQ YP MLB PA BF. Contributed reagents/materials/analysis tools: LD NM. Wrote the paper: MCC BF.
- 1. Musacchio A, Hardwick KG (2002) The spindle checkpoint: structural insights into dynamic signalling. Nat Rev Mol Cell Biol 3: 731–741.
- 2. Musacchio A, Salmon ED (2007) The spindle-assembly checkpoint in space and time. Nat Rev Mol Cell Biol 8: 379–393.
- 3. Li R, Murray AW (1991) Feedback control of mitosis in budding yeast. Cell 66: 519–531.
- 4. Hoyt MA, Totis L, Roberts BT (1991) S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell 66: 507–517.
- 5. Tang Z, Bharadwaj R, Li B, Yu H (2001) Mad2-Independent inhibition of APC Cdc20 by the mitotic checkpoint protein BubR1. Dev Cell 1: 227–237.
- 6. Fang G, Yu H, Kirschner MW (1998) The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation. Genes Dev 12: 1871–1883.
- 7. Sudakin V, Chan GK, Yen TJ (2001) Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2. J Cell Biol 154: 925–936.
- 8. Houben A, Schubert I (2003) DNA and proteins of plant centromeres. Curr Opin Plant Biol 6: 554–560.
- 9. Menges M, de Jager SM, Gruissem W, Murray JA (2005) Global analysis of the core cell cycle regulators of Arabidopsis identifies novel genes, reveals multiple and highly specific profiles of expression and provides a coherent model for plant cell cycle control. Plant J 41: 546–566.
- 10. Lermontova I, Fuchs J, Schubert I (2008) The Arabidopsis checkpoint protein Bub3.1 is essential for gametophyte development. Front Biosci 13: 5202–5211.
- 11. Yu HG, Muszynski MG, Kelly Dawe R (1999) The maize homologue of the cell cycle checkpoint protein MAD2 reveals kinetochore substructure and contrasting mitotic and meiotic localization patterns. J Cell Biol 145: 425–435.
- 12. Kimbara J, Endo TR, Nasuda S (2004) Characterization of the genes encoding for MAD2 homologues in wheat. Chromosome Res 12: 703–714.
- 13. Li L, Stoeckert CJ Jr, Roos DS (2003) OrthoMCL: identification of ortholog groups for eukaryotic genomes. Genome Res 13: 2178–2189.
- 14. Pfleger CM, Kirschner MW (2000) The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1. Genes Dev 14: 655–665.
- 15. Chan GK, Jablonski SA, Sudakin V, Hittle JC, Yen TJ (1999) Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC. J Cell Biol 146: 941–954.
- 16. Sczaniecka M, Feoktistova A, May KM, Chen JS, Blyth J, et al. (2008) The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C). J Biol Chem 283: 23039–23047.
- 17. Hardwick KG, Johnston RC, Smith DL, Murray AW (2000) MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p. J Cell Biol 148: 871–882.
- 18. Chen RH (2002) BubR1 is essential for kinetochore localization of other spindle checkpoint proteins and its phosphorylation requires Mad1. J Cell Biol 158: 487–496.
- 19. Fraschini R, Beretta A, Sironi L, Musacchio A, Lucchini G, et al. (2001) Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores. Embo J 20: 6648–6659.
- 20. Taylor SS, Ha E, McKeon F (1998) The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase. J Cell Biol 142: 1–11.
- 21. Li Y, Benezra R (1996) Identification of a human mitotic checkpoint gene: hsMAD2. Science 274: 246–248.
- 22. Stagljar I, Korostensky C, Johnsson N, te Heesen S (1998) A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo. Proc Natl Acad Sci USA 95: 5187–5192.
- 23. Hu CD, Chinenov Y, Kerppola TK (2002) Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell 9: 789–798.
- 24. Fang Y, Spector DL (2005) Centromere positioning and dynamics in living Arabidopsis plants. Mol Biol Cell 16: 5710–5718.
- 25. Lermontova I, Schubert V, Fuchs J, Klatte S, Macas J, et al. (2006) Loading of Arabidopsis centromeric histone CENH3 occurs mainly during G2 and requires the presence of the histone fold domain. Plant Cell 18: 2443–2451.
- 26. Talbert PB, Masuelli R, Tyagi AP, Comai L, Henikoff S (2002) Centromeric localization and adaptive evolution of an Arabidopsis histone H3 variant. Plant Cell 14: 1053–1066.
- 27. Basu J, Logarinho E, Herrmann S, Bousbaa H, Li Z, et al. (1998) Localization of the Drosophila checkpoint control protein Bub3 to the kinetochore requires Bub1 but not Zw10 or Rod. Chromosoma 107: 376–385.
- 28. Chen RH, Waters JC, Salmon ED, Murray AW (1996) Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores. Science 274: 242–246.
- 29. Martinez-Exposito MJ, Kaplan KB, Copeland J, Sorger PK (1999) Retention of the BUB3 checkpoint protein on lagging chromosomes. Proc Natl Acad Sci U S A 96: 8493–8498.
- 30. Genschik P, Criqui MC, Parmentier Y, Derevier A, Fleck J (1998) Cell cycle -dependent proteolysis in plants. Identification Of the destruction box pathway and metaphase arrest produced by the proteasome inhibitor mg132. Plant Cell 10: 2063–2076.
- 31. Criqui MC, Parmentier Y, Derevier A, Shen WH, Dong A, et al. (2000) Cell cycle-dependent proteolysis and ectopic overexpression of cyclin B1 in tobacco BY2 cells. Plant J 24: 763–773.
- 32. Howell BJ, Hoffman DB, Fang G, Murray AW, Salmon ED (2000) Visualization of Mad2 dynamics at kinetochores, along spindle fibers, and at spindle poles in living cells. J Cell Biol 150: 1233–1250.
- 33. Howell BJ, Moree B, Farrar EM, Stewart S, Fang G, et al. (2004) Spindle checkpoint protein dynamics at kinetochores in living cells. Curr Biol 14: 953–964.
- 34. Vos JW, Pieuchot L, Evrard JL, Janski N, Bergdoll M, et al. (2008) The plant TPX2 protein regulates prospindle assembly before nuclear envelope breakdown. Plant Cell 20: 2783–2797.
- 35. Austin JR 2nd, Segui-Simarro JM, Staehelin LA (2005) Quantitative analysis of changes in spatial distribution and plus-end geometry of microtubules involved in plant-cell cytokinesis. J Cell Sci 118: 3895–3903.
- 36. Van Damme D, Bouget FY, Van Poucke K, Inze D, Geelen D (2004) Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteins. Plant J 40: 386–398.
- 37. Caillaud MC, Lecomte P, Jammes F, Quentin M, Pagnotta S, et al. (2008) MAP65-3 microtubule-associated protein is essential for nematode-induced giant cell ontogenesis in Arabidopsis. Plant Cell 20: 423–437.
- 38. Lee YR, Giang HM, Liu B (2001) A novel plant kinesin-related protein specifically associates with the phragmoplast organelles. Plant Cell 13: 2427–2439.
- 39. Perez-Perez JM, Serralbo O, Vanstraelen M, Gonzalez C, Criqui MC, et al. (2008) Specialization of CDC27 function in the Arabidopsis thaliana anaphase-promoting complex (APC/C). Plant J 53: 78–89.
- 40. Abad P, Gouzy J, Aury JM, Castagnone-Sereno P, Danchin EG, et al. (2008) Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita. Nat Biotechnol 26: 909–915.
- 41. Deslandes L, Olivier J, Peeters N, Feng DX, Khounlotham M, et al. (2003) Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus. Proc Natl Acad Sci U S A 100: 8024–8029.
- 42. Voinnet O, Rivas S, Mestre P, Baulcombe D (2003) An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 33: 949–956.
- 43. Ritzenthaler C, Nebenfuhr A, Movafeghi A, Stussi-Garaud C, Behnia L, et al. (2002) Reevaluation of the effects of brefeldin A on plant cells using tobacco Bright Yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antisera. Plant Cell 14: 237–261.