In the early stages of development of the cellular slime mold Dictyostelium discoideum, chemotaxis toward cAMP plays a pivotal role in organizing discrete cells into a multicellular structure. In this process, a series of signaling molecules, such as G-protein-coupled cell surface receptors for cAMP, phosphatidylinositol metabolites, and cyclic nucleotides, function as the signal transducers for controlling dynamics of cytoskeleton. Differentiation-inducing factor-1 and -2 (DIF-1 and DIF-2) were originally identified as the factors (chlorinated alkylphenones) that induce Dictyostelium stalk cell differentiation, but it remained unknown whether the DIFs had any other physiologic functions.
To further elucidate the functions of DIFs, in the present study we investigated their effects on chemotaxis under various conditions. Quite interestingly, in shallow cAMP gradients, DIF-1 suppressed chemotaxis whereas DIF-2 promoted it greatly. Analyses with various mutants revealed that DIF-1 may inhibit chemotaxis, at least in part, via GbpB (a phosphodiesterase) and a decrease in the intracellular cGMP concentration ([cGMP]i). DIF-2, by contrast, may enhance chemotaxis, at least in part, via RegA (another phosphodiesterase) and an increase in [cGMP]i. Using null mutants for DimA and DimB, the transcription factors that are required for DIF-dependent prestalk differentiation, we also showed that the mechanisms for the modulation of chemotaxis by DIFs differ from those for the induction of cell differentiation by DIFs, at least in part.
Our findings indicate that DIF-1 and DIF-2 function as negative and positive modulators for Dictyostelium chemotaxis, respectively. To our knowledge, this is the first report in any organism of physiologic modulators (small molecules) for chemotaxis having differentiation-inducing activity.
Citation: Kuwayama H, Kubohara Y (2009) Differentiation-Inducing Factor-1 and -2 Function also as Modulators for Dictyostelium Chemotaxis. PLoS ONE 4(8): e6658. https://doi.org/10.1371/journal.pone.0006658
Editor: Paulo Lee Ho, Instituto Butantan, Brazil
Received: May 18, 2009; Accepted: July 16, 2009; Published: August 17, 2009
Copyright: © 2009 Kuwayama, Kubohara. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported in part by a special fund for tenure-track faculty members of the Institute of Biological Sciences at the University of Tsukuba (H.K.) and a Grant-in-aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (Y.K.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Chemotaxis—a fundamental cellular function for sensing the direction of extracellular stimuli and migrating toward or away from the source—is involved in various biological and physiologic events, such as lymphocyte homing, angiogenesis, embryogenesis, wound healing, and some inflammatory disorders –. Dictyostelium discoideum is an excellent model organism for the analysis of both chemotaxis and cell differentiation. These vegetative amoebae grow by eating bacteria, and upon starvation, start morphogenesis. During morphogenesis, the cells gather to form a slug-shaped multicellular aggregate that differentiates into two distinct cell types (prespore and prestalk cells). Eventually, the cells form a fruiting body consisting of spores and a multicellular stalk. Extracellular cAMP is not only an essential substance for cell differentiation but also a chemoattractant when the cells gather to form a multicellular aggregate , . Recently, Dictyostelium chemotaxis toward cAMP was shown to be regulated by several key signaling pathways involving phosphoinositide-3-kinase (PI3K), phospholipase A2 (PLA2), phospholipase C (PLC), and cGMP –, but the precise mechanisms controlling chemotaxis are unclear.
Differentiation-inducing factor-1, -2, and -3 (DIFs 1–3) were originally identified as the differentiation-inducing factors of stalk cells in D. discoideum , . DIF-1 is the most active species in inducing stalk cell differentiation, whereas DIF-3, the initial product in DIF-1 breakdown, has only 3.5% of the activity of DIF-1 , . In contrast, DIF-2 is neither a precursor nor a metabolite of DIF-1 in vivo and possesses as much as 40% of the specific activity of DIF-1 , , . Thus, DIF-2 is a curious compound, whose physiologic and specific roles, if any, are unknown.
It has been suggested that DIF-1 and DIF-2 may have roles other than inducing stalk cell differentiation during the early stage of development . To further elucidate the physiologic functions of DIFs in early development, in the present study we investigated the effects of DIF-1 and DIF-2 on chemotactic cell movement toward various concentrations of cAMP. We show here that in shallow cAMP gradients, DIF-1 and DIF-2 function as negative and positive modulators for chemotaxis, respectively.
Results and Discussion
Effects of DIFs on chemotaxis in Ax2 and HM1030 cells
We first studied Ax2 (wild type) cells starved for 4–8 h (Fig. 1B). When 10–100 nM droplets of cAMP were put on agar, 100 nM of DIFs did not significantly affect chemotactic movement. To our surprise, however, in Ax2 cells starved for 6–8 h, DIF-1 inhibited chemotaxis toward 0.1–1 nM cAMP, and DIF-2 promoted it greatly; thus, despite their structural similarity (Fig. 1A), the DIFs showed opposite effects on chemotaxis. These results suggest that DIF-1 and DIF-2 function as modulators of chemotactic cell movement toward cAMP in Ax2 cells that have been starved for more than 6 h.
(A) Chemical structure of DIF-1: 1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one and DIF-2: 1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)pentan-1-one. (B) Ax2 and HM1030 cells were starved for 4–8 h (as indicated in parentheses) in shake-culture, and cell droplets were spotted on PB agar containing 3 mM caffeine (Control) plus 100 nM DIF-1 or DIF-2. Cells were assayed for chemotaxis toward the indicated doses of cAMP (10 cell droplets were examined for each cAMP concentration). Data are the mean and s.d. (bars) of three independent experiments (n = 3). *P<0.05, as compared with Control.
We next examined the net effects of exogenous DIF-1 and DIF-2 on chemotaxis in dmtA- cells starved for 6 h; HM1030 (dmtA-) is a mutant strain that lacks the des-methyl-DIF-1 methyltransferase and thus cannot produce appreciable amounts of DIF-1 and DIF-2 , , although the mutant cells can gather to form multicellular aggregates and eventually fruiting bodies . In the presence or absence of exogenous DIFs (100 nM each), dmtA- and Ax2 cells showed similar chemotactic cell movement toward low concentrations of cAMP (Fig. 1B). Note, however, that 10–100 nM DIF-3, DMPH, and 2-MIDIF-1 did not affect chemotaxis toward 0.1–100 nM cAMP in either Ax2 or dmtA- cells (Fig. 2), indicating that the chemotaxis-modulating effects of DIF-1 and DIF-2 are highly specific to their chemical structures.
(A) DIF-3: 1-(3-chloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one. 2-MIDIF-1: 2-methoxy isomer of DIF-1. DMPH: 1-(2,6-dihydroxy-4-methoxyphenyl)hexan-1-one. (B) Ax2 and HM1030 cells starved for 6 h were spotted on PB agar containing 3 mM caffeine (Control) plus the indicated concentrations of DIF-3, DMPH, or 2-MIDIF-1 (2-MID) and assayed for chemotaxis toward the indicated doses of cAMP. Data are the mean and s.d. (bars) of three independent experiments (n = 3).
We next examined the effects of physiologic concentrations of DIFs (0.1–100 nM) on chemotaxis in dmtA- cells (Fig. 3A). DIF-1 at 3–100 nM inhibited chemotaxis toward 10 nM cAMP in a dose-dependent manner, whereas DIF-2 at 3–100 nM promoted chemotaxis toward 0.1 and 1 nM cAMP in a dose-dependent manner. We next assessed whether DIF-1 and DIF-2 competed with each other (Fig. 3B). As expected, DIF-1 at 10 nM inhibited chemotaxis toward 10 nM cAMP, and DIF-2 at 3–100 nM restored the DIF-1-inhibited chemotaxis in a dose-dependent manner. In clear contrast, DIF-2 at 10 nM promoted chemotaxis toward 0.1 and 1 nM cAMP, and DIF-1 at 3–100 nM dose-dependently suppressed the DIF-2-promoted chemotaxis.
(A) Effects of DIF concentrations on chemotaxis. HM1030 cells starved for 6 h were spotted on PB agar containing 3 mM caffeine plus various concentrations of DIF-1 (top) or DIF-2 (middle and bottom) and assayed for chemotaxis toward the doses of cAMP indicated in square brackets. Data are the mean and s.d. (bars) of three independent experiments (n = 3). (B) Combined effects of DIF-1 and DIF-2 on chemotaxis. HM1030 cells starved for 6 h were spotted on PB agar containing 3 mM caffeine plus 10 nM DIF-1 in combination with the various concentrations of DIF-2 (top) or plus 10 nM DIF-2 in combination with the various concentrations of DIF-1 (middle and bottom) and assayed for chemotaxis toward the doses of cAMP indicated in square brackets. Data are the mean and s.d. (bars) of three independent experiments (n = 3). *P<0.05, as compared with **Control.
Effects of DIFs on chemotaxis in dimA- and dimB-null cells
To investigate whether the DIFs modulate chemotaxis via cell differentiation, we examined the effects on chemotaxis in the null mutants for DimA and DimB, the transcription factors that are required for DIF-dependent prestalk differentiation –. Quite interestingly, chemotactic cell movement was significantly suppressed by DIF-1 and was well enhanced by DIF-2 in a dose-dependent manner in the dimA- mutant (Fig. 4). By contrast, DIF-1 did not affect chemotaxis in either the dimB- or dimA-/B- mutants, whereas DIF-2 promoted chemotaxis in all the mutants (Fig. 4). These results suggest that DimA is not essential for the actions of DIF-1 and DIF-2, whereas DimB is required for the action of DIF-1 but not of DIF-2. In other words, DIF-1 should suppress chemotaxis via DimB or DimB-inducible gene products, whereas DIF-2 promotes chemotaxis via a DimA/DimB-independent pathway. Thus, the mechanisms for the modulation of chemotaxis by DIFs differ from those for the induction of cell differentiation by DIFs, at least in part.
(A) Starved (for 6 h) dimA-, dimB-, and dimA-/B- cells were spotted on PB agar containing 3 mM caffeine (Control) plus 100 nM DIF-1 or DIF-2 and assayed for chemotaxis toward the indicated doses of cAMP. Data are the mean and s.d. (bars) of three independent experiments (n = 3). (B) Starved dimA-, dimB-, and dimA-/B- cells were spotted on PB agar containing 3 mM caffeine plus the indicated concentrations of DIF-1 or DIF-2 and assayed for chemotaxis toward the doses of cAMP indicated above in square brackets. Data are the mean and s.d. (bars) of three independent experiments (n = 3). *P<0.05, as compared with Control. (C) Expression levels of regA and gbpB. Cells were starved for 6 h, and RNAs collected from the cells were used for semi-quantitative RT-PCR to detect regA, gbpB, and rnlA (internal control).
Effects of DIFs on chemotaxis in regA- and gbpB-null cells
We then examined the effects of the DIFs on chemotaxis in a variety of mutants lacking the genes required for normal chemotaxis (Fig. 5). Note that chemotaxis in shallow cAMP gradients was greatly impaired in pi3k1-/2-, pten-, plaA-, and gca-/sgc- cells, in which the chemotaxis-modulating effects of DIFs were not observed (Fig. 5). This finding suggests that the activities of the PI3-kinases, PTEN, PLA2, and the guanylylcyclases should be required for normal chemotaxis in shallow gradients. However, because pi-kinases/pten sextuple null (pi3ks-/pten-) cells exhibited a normal chemotactic response to cAMP in the presence or absence of DIFs (Fig. 5), the PI3-kinases and PTEN are likely not essential for the modulation of chemotaxis by DIFs.
Various mutants starved for 6 h were spotted on PB agar containing 3 mM caffeine (Control) plus 100 nM DIF-1 or DIF-2 and assayed for chemotaxis toward the indicated doses of cAMP. Data are the mean and s.d. (bars) of three independent experiments (n = 3). *P<0.05, as compared with Control. pi3k1 and pi3k2: genes of phosphoinositide-3 kinase. pten: phasphotase and tensin homolog gene. plaA: phospholipase A2 gene. gca and sgc: genes of guanylyl cyclase A and soluble guanylyl cyclase. gbpA, gbpB, and gbpD: genes of cGMP-binding protein A, B, and D, respectively. Note that chemotaxis toward low concentrations of cAMP was impaired in pi3k1-/2-, pten-, plaA-, and gca-/sgc-, in which the chemotaxis-modulating effects of DIFs were not observed, and that DIFs showed essentially the same effects in gbpA-/B- as seen in gbpB- cells (Fig. 6A).
Our results in mutants lacking the cyclic nucleotide phophodiesterase (PDE) genes regA and gbpB ,  were particularly striking. To our surprise, DIFs at 100 nM showed the same effects of inhibiting chemotaxis in regA- cells and of enhancing chemotaxis in gbpB- cells (Fig. 6A). In clear contrast, however, in regA- cells, DIF-1 at 10 nM inhibited chemotaxis but DIF-2 at 10 nM did not affect chemotaxis (Fig. 6B), whereas in gbpB- cells, DIF-2 at 10 nM enhanced chemotaxis but DIF-1 at 10 nM did not affect chemotaxis (Fig. 6B). These results strongly suggest that the pathways by which DIF-1 and DIF-2 modulate chemotaxis involve GbpB (PDE for cGMP)  and RegA (PDE for cAMP?) , respectively, and that DIF-1 and DIF-2 at high concentrations (e.g., 100 nM) may have the potential to cross-affect the other pathway (Fig. 7B). More precisely, DIF-1 may inhibit chemotaxis, at least in part, via GbpB activation and a subsequent decrease in the intracellular cGMP concentration ([cGMP]i), whereas DIF-2 may enhance chemotaxis, at least in part, via a RegA-dependent pathway.
(A, B) Cells starved for 6 h were spotted on PB agar containing 3 mM caffeine (Control) plus 100 nM (A) or 10 nM (B) of DIF-1 or DIF-2 and assayed for chemotaxis toward the indicated doses of cAMP (top). Starved cells in shake-culture were stimulated with 0.3 nM cAMP (final concentration) in the presence of 3 mM caffeine (Control) plus 100 nM (A) or 10 nM (B) of DIF-1 or DIF-2, and aliquots of the cells were collected for assay of cGMP contents (bottom). Data are the mean and s.d. (bars) of three independent experiments (n = 3). *P<0.05, as compared with Control.
A series of signaling molecules, such as G-protein-coupled cell surface receptors for cAMP, phosphatidylinositol metabolites, and cyclic nucleotides, function as the signal transducers to control the dynamics of the cytoskeleton. DIFs modulate chemotaxis by affecting [cGMP]i. cAR1, G-protein-coupled cAMP receptor; PLA2, phospholipase A2; PLC, phospholipase C; PIP2, phosphatidylinositol (4,5)-bisphosphate; PIP3, phosphatidylinositol (3,4,5)-trisphosphate; PTEN, phosphatase and tensin homolog; DAG, diacylglycerol; IP3, inositol (1,4,5)-triphosphate; GCase, guanylyl cyclases. (B) Proposed model for how DIFs modulate Dictyostelium chemotaxis. DIF-1 inhibits chemotaxis, at least in part, via activation of GbpB and a subsequent decrease in [cGMP]i, whereas DIF-2 enhances chemotaxis, at least in part, via a RegA-dependent pathway and a subsequent increase in [cGMP]i. At high concentrations (e.g., 100 nM), DIF-1 and DIF-2 may cross-affect the other pathway to some extent; therefore, DIFs showed the same effects of inhibiting chemotaxis in regA- cells and of enhancing chemotaxis in gbpB- cells (Fig. 6A).
Effects of DIFs on [cGMP]i
To illustrate our hypothesis for the actions of DIFs (Fig. 7), we further examined the effects of DIFs on [cGMP]i in Ax2, regA-, and gbpB- cells (Fig. 6A, B). Stimulation with cAMP induced a transient increase in [cGMP]i within 20 s that was significantly inhibited by 10–100 nM DIF-1 and was enhanced by 10–100 nM DIF-2 in Ax2 cells. In regA- cells, as expected, the cAMP-induced increase in [cGMP]i was inhibited by 10–100 nM DIF-1 or 100 nM DIF-2 and was not affected by 10 nM DIF-2. In gbpB- cells, in contrast, the cAMP-induced increase in [cGMP]i was enhanced by 10–100 nM DIF-2 or 100 nM DIF-1 but was not affected by 10 nM DIF-1. These results strongly support our hypothesis for the actions of DIFs in chemotaxis (Fig. 7).
Expression of regA and gbpB in dimA- and dimB-null cells
To confirm that RegA and GbpB are involved in DIF-modulated chemotaxis, we investigated the relation between responsiveness to DIFs and expression of the PDEs in dimA- and dimB- cells (Fig. 4C). Indeed, regA was expressed in all mutants in which chemotaxis was enhanced by DIF-2. Furthermore, gbpB mRNA was expressed in dimA- cells in which chemotaxis was inhibited by DIF-1, whereas gbpB mRNA was not expressed in dimB- cells in which chemotaxis was not affected by DIF-1. These results support our model in which the effects of DIF-1 and DIF-2 are GbpB- and RegA-dependent, respectively.
Various indirect evidence suggests that DIFs may have novel functions in controlling cellular movement (e.g., chemotaxis) during early development. First, as measured by a bioassay based on stalk cell induction, a major rise in DIF levels occurs at the end of aggregation, and low levels of DIF activity are detected during the early aggregation stage , . In addition, DmtA is detectable at 3–6 h of development . Second, cAMP relay is inhibited by DIF-1 in aggregation-competent cells . Third, as measured by monitoring light-scattering cellular responses and cyclic nucleotide production in in vitro cell suspensions, DIFs may affect cell shape (or cohesion) and the levels of cAMP and cGMP in early stages of development .
Here, we have elucidated the novel functions of DIFs. We found that DIF-1 and DIF-2 function as negative and positive modulators of D. discoideum chemotaxis, respectively, in shallow cAMP gradients. Because we performed our experiments in the presence of caffeine, an inhibitor of endogenous cAMP production (cAMP relay), we were able to elucidate the net and intrinsic effects of DIFs on cellular cGMP levels and chemotaxis toward very low levels of exogenous cAMP; thus, the mechanisms underlying the actions of DIFs in chemotaxis in vivo would be more complicated than described in Fig. 7B.
In our model, we assume that GbpB activation by DIF-1 suppresses cAMP-stimulated cGMP production and that DIF-2 somehow promotes cAMP-stimulated cGMP production via a RegA-dependent pathway. Bosgraaf et al.  showed that GbpB is a PDE for cGMP, which supports our model with respect to the GbpB-dependent action of DIF-1. However, because Shaulsky et al.  have shown with recombinant RegA that RegA is a PDE that is specific to cAMP but not to cGMP, RegA might affect cellular cGMP levels indirectly. Still, it is possible that RegA directly degrades cGMP in vivo in the presence of some co-factor or co-factors; if so, DIF-2 would promote chemotaxis via RegA inhibition and a subsequent increase in [cGMP]i. At any rate, DIF-2 promotes cAMP-stimulated cGMP production and chemotaxis when RegA is present (Fig. 4C, 6), and our model agrees well with a general notion that intracellular cGMP regulates myosin filament formation and thus chemotaxis –. To our knowledge, this is the first report in any organism of physiologic modulators for chemotaxis having differentiation-inducing activity. Furthermore, our findings suggest that cell differentiation inducers (small molecules) may function as key modulators for chemotaxis and thus for morphogenesis in other organisms as well.
Although it is not known how DIFs control RegA and GbpB activity, because DIFs have been shown to directly inhibit calmodulin-dependent PDE1 (specific to cAMP and cGMP) and suppress cell growth in mammalian cells , , RegA or GbpB may be direct targets of DIFs in D. discoideum. Furthermore, DIFs may modulate mammalian chemotaxis via PDE1 or other PDEs; if so, some DIF derivatives might be utilized as drugs to control mammalian chemotaxis for basic research and therapeutic purposes.
Because joining a multicelluar aggregate and differentiating into spores may be essential to survival and reproduction in the social amoeba D. discoideum, rapid and slow movement toward aggregates modulated by DIFs may affect fitness. In nature, D. discoideum cells would hardly synchronize the start of development (starvation); thus, DIFs produced by senior cells would considerably affect junior cells during development. The physiologic and evolutionary significance of the modulators of chemotaxis and the detailed mechanisms of their actions should be elucidated further.g
Materials and Methods
Dictyostelium discoideum strains and DIF
The dmtA- , regA- , gbpA-, gbpB-, gbpA-/gbpB-, gbpD- , , , gcA-/sgc- , pi3k1-/pi3k2- , pten- , dimA-, dimB-, and dimA-/B- – strains have been described previously. The plaA- strain was generated by transforming Ax2 cells with a gene disruption construct made by inserting the bsr cassette into the EcoRV site of the genomic region of the plaA ORF. DIFs were obtained as previously described , .
Cells were cultured at 21°C in HL5 medium with 100 µg/mL streptomycin sulfate and 100 units/mL benzylpenicillin potassium, as previously described . For culturing the gene null transformants, the HL5 medium was supplemented with 10 µg/mL blasticidin S.
The chemotaxis assay was performed by the small population assay as previously described with a few modifications . Cells were harvested by centrifugation (350×g) for 2 min, washed in phosphate buffer (PB) (10 mM KH2PO4/Na2HPO4, pH 6.5), and starved at a density of 1×107 cells/mL in PB buffer for 1 h. Then cAMP was added for 5 h in a pulsatile fashion every 6 min to a final concentration of 30 nM. Starved cells were resuspended in PB, washed twice in PB, and resuspended in PB to a final concentration of 5×106 cells/mL. Ten<0.2-µL droplets of starved cells were placed on a plate containing 10 mL of non-nutrient hydrophobic agar (10 mM KH2PO4/Na2HPO4, pH 6.5, 0.7% hydrophobic agar containing 3 mM caffeine). Chemotaxis toward cAMP was tested after 30 min by placing a second 0.1-µL droplet, with the indicated amount of cAMP, next to the droplet of cells. The distribution of the cells in the droplet was observed after 30 and 60 min, and they were scored ‘positive’ when at least twice as many cells were pressed against the side of the population closer to the higher cAMP concentration as against the other side of the droplet. The percentage of ‘positive’ droplets was assessed, and the mean values of three independent experiments are presented with standard deviations (s.d.).
cGMP was assayed as described previously by using the starved cells resuspended in PB containing 3 mM caffeine .
Semi-quantitative RT–PCR gene expression analysis
Total RNA was prepared by use of RNeasy mini kits (Qiagen, Hilden, Germany). cDNA was synthesized by Superscript II (Invitrogen, Carlsbad, CA) with a random DNA hexamer. Semi-quantitative RT–PCR was performed by using a KOD plus (TOYOBO, Osaka, Japan). PCRs were carried out with the following program: one cycle of 120 s at 94°C followed by 28 cycles (for rnlA and regA) and 35 cycles (for gbpB) consisting of 20 s at 94°C, 30 s at 55°C, 60 s at 65°C, and by one cycle of 60 s at 65°C. The following primer sets were used: regA, GCAAGAATCGCAGCGGATTTC and TGTATGCTTGCCAATTTTCACG; gbpB, CTTCGGTGGGTACAGTTGTG and AAGCAAACGTCAGTCTCTGC; rnlA, GAGGCGCTGGTGAAATAGTAAG and ACTCTTTAGAAGGTTACCGCCC (mitochondrial large subunit rRNA; internal control).
We thank Dr. R.R. Kay for providing us with a pi3ks-/pten- strain and critical reading of the manuscript. We also thank the National Bio-Resource Project (Nenkin) in Japan for providing us with dmtA-, regA-, gbpA-, gbpB-, gbpA-/gbpB-, gcA-/sgc-, gbpD-, pi3k1-/pi3k2-, pten-, dimA-, dimB-, and dimA-/B- strains.
Conceived and designed the experiments: HK YK. Performed the experiments: HK YK. Analyzed the data: HK YK. Wrote the paper: HK YK.
- 1. Baggiolini M (1998) Chemokines and leukocyte traffic. Nature 392: 565–568.M. Baggiolini1998Chemokines and leukocyte traffic.Nature392565568
- 2. Campbell JJ, Butcher EC (2000) Chemokines in tissue-specific and microenvironment-specific lymphocyte homing. Curr Opin Immunol 12: 336–341.JJ CampbellEC Butcher2000Chemokines in tissue-specific and microenvironment-specific lymphocyte homing.Curr Opin Immunol12336341
- 3. Wardlaw AJ, Brightling C, Green R, Woltmann G, Pavord I (2000) Eosinophils in asthma and other allergic diseases. Br Med Bull 56: 985–1003.AJ WardlawC. BrightlingR. GreenG. WoltmannI. Pavord2000Eosinophils in asthma and other allergic diseases.Br Med Bull569851003
- 4. Konijn TM, van de Meene JGC, Bonner JT, Barkley DS (1967) The acrasin activity of adenosine-3′,5′-cyclic phosphate. Proc Natl Acad Sci USA 58: 1152–1154.TM KonijnJGC van de MeeneJT BonnerDS Barkley1967The acrasin activity of adenosine-3′,5′-cyclic phosphate.Proc Natl Acad Sci USA5811521154
- 5. Darmon M, Brachet P, Pereira da Silva LH (1975) Chemotactic signals induce cell differentiation in Dictyostelium discoideum. Proc Natl Acad Sci USA 72: 3163–3166.M. DarmonP. BrachetLH Pereira da Silva1975Chemotactic signals induce cell differentiation in Dictyostelium discoideum.Proc Natl Acad Sci USA7231633166
- 6. Funamoto S, Meili R, Lee S, Parry L, Firtel RA (2002) Spatial and temporal regulation of 3-phosphoinositides by PI3-kinase and PTEN mediates chemotaxis. Cell 109: 611–623.S. FunamotoR. MeiliS. LeeL. ParryRA Firtel2002Spatial and temporal regulation of 3-phosphoinositides by PI3-kinase and PTEN mediates chemotaxis.Cell109611623
- 7. Iijima M, Devreotes PN (2002) Tumor suppressor PTEN mediates sensing of chemoattractant gradients. Cell 109: 599–610.M. IijimaPN Devreotes2002Tumor suppressor PTEN mediates sensing of chemoattractant gradients.Cell109599610
- 8. Willard SS, Devereotes PN (2006) Signaling pathways mediating chemotaxis in the social amoeba, Dictyostelium discoideum. Eur J Cell Biol 85: 897–904.SS WillardPN Devereotes2006Signaling pathways mediating chemotaxis in the social amoeba, Dictyostelium discoideum.Eur J Cell Biol85897904
- 9. Chen L, Iijima M, Tang M, Landree MA, Huang YE, et al. (2007) PLA2 and PI3K/PTEN pathways act in parallel to mediate chemotaxis. Dev Cell 12: 603–614.L. ChenM. IijimaM. TangMA LandreeYE Huang2007PLA2 and PI3K/PTEN pathways act in parallel to mediate chemotaxis.Dev Cell12603614
- 10. Korthol A, King JS, Keizer-Gunnink I, Harwood AJ, van Haastert PJM (2007) Phospholipase C regulation of phosphatidylinositol 3,4,5-trisphosphate-mediated chemotaxis. Mol Biol Cell 18: 4772–4779.A. KortholJS KingI. Keizer-GunninkAJ HarwoodPJM van Haastert2007Phospholipase C regulation of phosphatidylinositol 3,4,5-trisphosphate-mediated chemotaxis.Mol Biol Cell1847724779
- 11. Janetopoulos C, Firtel RA (2008) Directional sensing during chemotaxis. FEBS Lett 582: 2075–2085.C. JanetopoulosRA Firtel2008Directional sensing during chemotaxis.FEBS Lett58220752085
- 12. Kölsch V, Charest PG, Firtel RA (2008) The regulation of cell motility and chemotaxis by phospholipid signaling. J Cell Sci 151: 551–559.V. KölschPG CharestRA Firtel2008The regulation of cell motility and chemotaxis by phospholipid signaling.J Cell Sci151551559
- 13. van Haastert PJM, Keizer-Gunnink I, Kotholt A (2007) Essential role of PI3-kinase and phospholipase A2 in Dictyostelium discoideum chemotaxis. J Cell Biol 177: 809–816.PJM van HaastertI. Keizer-GunninkA. Kotholt2007Essential role of PI3-kinase and phospholipase A2 in Dictyostelium discoideum chemotaxis.J Cell Biol177809816
- 14. Vetlman DM, Keizer-Gunnik I, van Haastert PJM (2008) Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum. J Cell Biol 180: 747–753.DM VetlmanI. Keizer-GunnikPJM van Haastert2008Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum.J Cell Biol180747753
- 15. Town DD, Gross JD, Kay RR (1976) Cell differentiation without morphogenesis in Dictyostelium discoideum. Nature 262: 717–719.DD TownJD GrossRR Kay1976Cell differentiation without morphogenesis in Dictyostelium discoideum.Nature262717719
- 16. Morris HR, Taylor GW, Masento MS, Jermyn KA, Kay RR (1987) Chemical structure of the morphogen differentiation inducing factor from Dictyostelium discoideum. Nature 328: 811–814.HR MorrisGW TaylorMS MasentoKA JermynRR Kay1987Chemical structure of the morphogen differentiation inducing factor from Dictyostelium discoideum.Nature328811814
- 17. Kay RR, Flatman P, Thompson CRL (1999) DIF signalling and cell fate. Semin Cell Dev Biol 10: 577–585.RR KayP. FlatmanCRL Thompson1999DIF signalling and cell fate.Semin Cell Dev Biol10577585
- 18. Wurster B, Kay RR (1990) New roles for DIF? Effects on early development in Dictyostelium. Dev Biol 140: 189–195.B. WursterRR Kay1990New roles for DIF? Effects on early development in Dictyostelium.Dev Biol140189195
- 19. Masento MS, Morris HR, Taylor GW, Johnson SJ, Skapski AC, et al. (1988) Differentiation-inducing factor from the slime mould Dictyostelium discoideum and its analogues. Biochem J 256: 23–28.MS MasentoHR MorrisGW TaylorSJ JohnsonAC Skapski1988Differentiation-inducing factor from the slime mould Dictyostelium discoideum and its analogues.Biochem J2562328
- 20. Morris HR, Masento MS, Taylor GW, Jermyn KA, Kay RR (1988) Structure elucidation of two differentiation inducing factors (DIF-2 and DIF-3) from the cellular slime mould Dictyostelium discoideum. Biochem J 249: 903–906.HR MorrisMS MasentoGW TaylorKA JermynRR Kay1988Structure elucidation of two differentiation inducing factors (DIF-2 and DIF-3) from the cellular slime mould Dictyostelium discoideum.Biochem J249903906
- 21. Thompson CRL, Kay RR (2000) The role of DIF-1 signaling in Dictyostelium development. Mol Cell 6: 1509–1514.CRL ThompsonRR Kay2000The role of DIF-1 signaling in Dictyostelium development.Mol Cell615091514
- 22. Saito T, Taylor GW, Yang J, Neuhaus D, Stetsenko D, et al. (2006) Identification of new differentiation inducing factors from Dictyostelium discoideum. Biochim Biophys Acta 1760: 754–761.T. SaitoGW TaylorJ. YangD. NeuhausD. Stetsenko2006Identification of new differentiation inducing factors from Dictyostelium discoideum.Biochim Biophys Acta1760754761
- 23. Thompson CRL, Fu Q, Buhay C, Kay RR, Shaulsky G (2004) A bZIP/bRLZ transcription factor required for DIF signaling in Dictyostelium. Development 131: 513–523.CRL ThompsonQ. FuC. BuhayRR KayG. Shaulsky2004A bZIP/bRLZ transcription factor required for DIF signaling in Dictyostelium.Development131513523
- 24. Huang E, Blagg SL, Keller T, Katoh M, Shaulsky G, et al. (2006) bZIP transcription factor interactions regulate DIF responses in Dictyostelium. Development 133: 449–458.E. HuangSL BlaggT. KellerM. KatohG. Shaulsky2006bZIP transcription factor interactions regulate DIF responses in Dictyostelium.Development133449458
- 25. Zhukovskaya NV, Fukuzawa M, Yamada Y, Araki T, Williams JG (2006) The Dictyostelium bZIP transcription factor DimB regulates prestalk-specific gene expression. Development 133: 439–448.NV ZhukovskayaM. FukuzawaY. YamadaT. ArakiJG Williams2006The Dictyostelium bZIP transcription factor DimB regulates prestalk-specific gene expression.Development133439448
- 26. Shaulsky G, Escalante R, Loomis WF (1996) Developmental signal transduction pathways uncovered by genetic suppressors. Proc Natl Acad Sci USA 93: 15260–15265.G. ShaulskyR. EscalanteWF Loomis1996Developmental signal transduction pathways uncovered by genetic suppressors.Proc Natl Acad Sci USA931526015265
- 27. Bosgraaf L, Russcher H, Snippe H, Bader S, Wind J, et al. (2002) Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in Dictyostelium. Mol Biol Cell 13: 3878–3889.L. BosgraafH. RusscherH. SnippeS. BaderJ. Wind2002Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in Dictyostelium.Mol Biol Cell1338783889
- 28. Shaulsky G, Fuller D, Loomis WF (1998) A cAMP-phosphodiesterase controls PKA-dependent differentiation. Development 125: 691–699.G. ShaulskyD. FullerWF Loomis1998A cAMP-phosphodiesterase controls PKA-dependent differentiation.Development125691699
- 29. Brookman JJ, Town CD, Jermyn KA, Kay RR (1982) Developmental regulation of a stalk cell differentiation inducing factor in Dictyostelium discoideum. Dev Biol 91: 191–196.JJ BrookmanCD TownKA JermynRR Kay1982Developmental regulation of a stalk cell differentiation inducing factor in Dictyostelium discoideum.Dev Biol91191196
- 30. Sobolewski A, Neave N, Weeks G (1983) The induction of stalk cell differentiation in submerged monolayers of Dictyostelium discoideum. Differentiation 25: 93–100.A. SobolewskiN. NeaveG. Weeks1983The induction of stalk cell differentiation in submerged monolayers of Dictyostelium discoideum.Differentiation2593100
- 31. Wang M, van Haastert PJM, Schaap P (1986) Multiple effects of differentiation inducing factor on prespore differentiation and cyclic-AMP signal transduction in Dictyostelium. Differentiation 33: 24–28.M. WangPJM van HaastertP. Schaap1986Multiple effects of differentiation inducing factor on prespore differentiation and cyclic-AMP signal transduction in Dictyostelium.Differentiation332428
- 32. Liu G, Newell PC (1993) Role of cyclic GMP in signal transduction to cytoskeletal myosin. Sympl Soc Exp Biol 47: 283–295.G. LiuPC Newell1993Role of cyclic GMP in signal transduction to cytoskeletal myosin.Sympl Soc Exp Biol47283295
- 33. Bosgraaf L, Russcher H, Smith JL, Wessels D, Soll DR, et al. (2002) A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium. EMBO J 21: 4560–4570.L. BosgraafH. RusscherJL SmithD. WesselsDR Soll2002A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium.EMBO J2145604570
- 34. Goldberg JM, Bosgraaf L, van Haastert PJM, Smith L (2002) Identification of four candidate cGMP targets in Dictyostelium. Proc Natl Acad Sci USA 99: 6749–6754.JM GoldbergL. BosgraafPJM van HaastertL. Smith2002Identification of four candidate cGMP targets in Dictyostelium.Proc Natl Acad Sci USA9967496754
- 35. Postma M, Bosgraaf L, Loovers HM, van Haastert PJM (2004) Chemotaxis: signalling modules join hands at front and tail. EMBO Rep 5: 35–40.M. PostmaL. BosgraafHM LooversPJM van Haastert2004Chemotaxis: signalling modules join hands at front and tail.EMBO Rep53540
- 36. Bosgraaf L, van Haastert PJM (2006) The regulation of myosin II in Dictyostelium. Eur J Cell Biol 85: 969–979.L. BosgraafPJM van Haastert2006The regulation of myosin II in Dictyostelium.Eur J Cell Biol85969979
- 37. Kubohara Y (1999) Effects of differentiation-inducing factors (DIFs) of Dictyostelium discoideum on the human leukemia K562 cells: DIF-3 is the most potent anti-leukemic agent. Eur J Pharmacol 381: 57–62.Y. Kubohara1999Effects of differentiation-inducing factors (DIFs) of Dictyostelium discoideum on the human leukemia K562 cells: DIF-3 is the most potent anti-leukemic agent.Eur J Pharmacol3815762
- 38. Shimizu K, Murata T, Tagawa T, Takahashi K, Ishikawa R, et al. (2004) Calmodulin-dependent cyclic nucleotide phosphodiesterase (PDE1) is a pharmacological target of differentiation-inducing factor-1, an anti-tumor agent isolated from Dictyostelium. Cancer Res 64: 2568–2571.K. ShimizuT. MurataT. TagawaK. TakahashiR. Ishikawa2004Calmodulin-dependent cyclic nucleotide phosphodiesterase (PDE1) is a pharmacological target of differentiation-inducing factor-1, an anti-tumor agent isolated from Dictyostelium.Cancer Res6425682571
- 39. Roelofs J, van Haastert PJM (2002) Deducing the origin of soluble adenylyl cyclase, a gene lost in multiple lineages. Mol Biol Evol 19: 2239–2246.J. RoelofsPJM van Haastert2002Deducing the origin of soluble adenylyl cyclase, a gene lost in multiple lineages.Mol Biol Evol1922392246
- 40. Kubohara Y, Hosaka K (1999) The putative morphogen, DIF-1, of Dictyostelium discoideum activates Akt/PKB in human leukemia K562 cells. Biochem Biophys Res Commun 263: 790–796.Y. KuboharaK. Hosaka1999The putative morphogen, DIF-1, of Dictyostelium discoideum activates Akt/PKB in human leukemia K562 cells.Biochem Biophys Res Commun263790796
- 41. Kuwayama H, Obara S, Morio T, Katoh M, Urushihara H, et al. (2002) PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors. Nucleic Acid Res 30: E2.H. KuwayamaS. ObaraT. MorioM. KatohH. Urushihara2002PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors.Nucleic Acid Res30E2
- 42. Kuwayama H, Ishida S, van Haastert PJM (1993) Non-chemotactic Dictyostelium discoideum mutants with altered cGMP signal transduction. J Cell Biol 123: 1453–1462.H. KuwayamaS. IshidaPJM van Haastert1993Non-chemotactic Dictyostelium discoideum mutants with altered cGMP signal transduction.J Cell Biol12314531462