Ruminococcus flavefaciens is a predominant cellulolytic rumen bacterium, which forms a multi-enzyme cellulosome complex that could play an integral role in the ability of this bacterium to degrade plant cell wall polysaccharides. Identifying the major enzyme types involved in plant cell wall degradation is essential for gaining a better understanding of the cellulolytic capabilities of this organism as well as highlighting potential enzymes for application in improvement of livestock nutrition and for conversion of cellulosic biomass to liquid fuels.
The R. flavefaciens FD-1 genome was sequenced to 29x-coverage, based on pulsed-field gel electrophoresis estimates (4.4 Mb), and assembled into 119 contigs providing 4,576,399 bp of unique sequence. As much as 87.1% of the genome encodes ORFs, tRNA, rRNAs, or repeats. The GC content was calculated at 45%. A total of 4,339 ORFs was detected with an average gene length of 918 bp. The cellulosome model for R. flavefaciens was further refined by sequence analysis, with at least 225 dockerin-containing ORFs, including previously characterized cohesin-containing scaffoldin molecules. These dockerin-containing ORFs encode a variety of catalytic modules including glycoside hydrolases (GHs), polysaccharide lyases, and carbohydrate esterases. Additionally, 56 ORFs encode proteins that contain carbohydrate-binding modules (CBMs). Functional microarray analysis of the genome revealed that 56 of the cellulosome-associated ORFs were up-regulated, 14 were down-regulated, 135 were unaffected, when R. flavefaciens FD-1 was grown on cellulose versus cellobiose. Three multi-modular xylanases (ORF01222, ORF03896, and ORF01315) exhibited the highest levels of up-regulation.
The genomic evidence indicates that R. flavefaciens FD-1 has the largest known number of fiber-degrading enzymes likely to be arranged in a cellulosome architecture. Functional analysis of the genome has revealed that the growth substrate drives expression of enzymes predicted to be involved in carbohydrate metabolism as well as expression and assembly of key cellulosomal enzyme components.
Citation: Berg Miller ME, Antonopoulos DA, Rincon MT, Band M, Bari A, Akraiko T, et al. (2009) Diversity and Strain Specificity of Plant Cell Wall Degrading Enzymes Revealed by the Draft Genome of Ruminococcus flavefaciens FD-1. PLoS ONE 4(8): e6650. https://doi.org/10.1371/journal.pone.0006650
Editor: Niyaz Ahmed, University of Hyderabad, India
Received: May 4, 2009; Accepted: July 7, 2009; Published: August 14, 2009
Copyright: © 2009 Berg Miller et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by a USDA grant for Functional Genomics of Ruminococcus flavefaciens FD-1 (Grant No. 2002-35206-11634) and by grants from the Israel Science Foundation (Grant Nos 422/05, 159/07 and 291/08) and the United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel. We also thank the The North American Consortium for Genomics of Fibrolytic Ruminal Bacteria which was supported by the Initiative for Future Agriculture and Food Systems, Grant no. 2000-52100-9618 and Grant No 2001-52100-11330, from the USDA Cooperative State Research, Education, and Extension Service's National Research Initiative Competitive Grants Program for support for DA. HJF would like to acknowledge support from the Scottish Government Rural Environment Research and Analysis Directorate. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Ruminococci are cellulolytic Gram-positive cocci in the order ‘Clostridiales’, which inhabit the rumen community. They are responsible for degrading cellulosic plant cell wall material, and also for solubilizing components that can be utilized by other rumen bacteria . Members of the Ruminococcus genus were first described by A. K. Sijpesteijn in the early part of the twentieth century which were followed by equivalent descriptions by R. E. Hungate , . The R. flavefaciens FD-1 strain was first isolated by Marvin P. Bryant from a bolus containing ruminal microorganisms used to improve rumen function in calves . Although the R. flavefaciens type strain is C94, its cellulolytic activity is much lower than that of FD-1, particularly on more crystalline forms of cellulose . R. flavefaciens strains are known to vary widely in their activities against intact plant cell wall material, and against different forms of cellulose, but many strains share with FD-1 the ability to attack highly crystalline forms of cellulose . Most R. flavefaciens strains exhibit a preference for more complex sugars, as evidenced by the uptake of cellobiose but the absence of an uptake system for glucose . R. flavefaciens-related bacteria are also thought to play a role in plant cell wall polysaccharide digestion in the large intestine in herbivorous mammals and in man .
The diversity and organization of cellulases and other proteins involved in plant cell wall breakdown by rumen cellulolytic bacteria is fundamental to understanding how ruminants extract energy from their diet. The cellulolytic enzyme system from R. flavefaciens FD-1 has been shown to include a variety of exo-β-1,4-glucanases, endo-β-1,4-glucanases and cellodextrinases , , , . Difficulties were encountered in initial fractionation of these enzymes as they appeared to exist in high-molecular-weight protein complexes resembling cellulosomes , , and enzymatic activity was lost rapidly when the complexes were disrupted . Individual β-glucanase genes (celA, celB, celC, and celD) were cloned from R. flavefaciens FD-1 with a view to studying their regulation , , , , . Meanwhile, parallel studies in the related R. flavefaciens strain 17 also led to the sequence analysis of a number of xylanases and cellulases. This revealed the presence of multiple catalytic modules in xylanases , ,  and the presence of non-catalytic dockerins ,  and of substrate-binding modules  in both cellulases and xylanases. The hypothesis that these dockerin-containing enzymes are organized into cellulosomes was supported by the discovery of the sca cluster of genes in R. flavefaciens 17 that encodes the cohesin-containing scaffolding or anchoring proteins ScaA, B, C and E , , , . Evidence was obtained in R. flavefaciens 17 that many enzymes are assembled into the cellulosome complex via cohesin-dockerin interactions involving the ScaA “scaffoldin” protein, while other, currently unknown, proteins appear to be accommodated via the ScaC adaptor protein , . ScaA in turn binds via its C-terminal dockerin to ScaB, which is held into the cell surface via another cohesin-dockerin interaction with the cell-wall anchored protein ScaE , . The homologous sca cluster has now been identified in R. flavefaciens FD-1 and shows close alignment in gene order with that in R. flavefaciens 17, although interesting interstrain differences exist in the modular structures of ScaA and ScaB . Experimental verification of specific cohesin-dockerin interactions indicates that a broadly similar cellulosome organization exists in R. flavefaciens FD-1 and 17 . Genes encoding several molecular chaperones (groES, groEL, and dnaK) have also been described from R. flavefaciens FD-1 that could be involved in the assembly of cellulosome-like structures .
Genome sequencing of R. flavefaciens FD-1 offers the prospect of obtaining far more extensive information on the range and diversity of enzymatic and structural components of the cellulosome, on its organization, range of cohesin-dockerin interactions, and on the regulation and assembly of cellulosomal subunits. At the same time, significant information is obtained on non-cellulosomal proteins. Here, the genome of R. flavefaciens FD-1 was sequenced to approximately 29×-coverage, and the resulting collection of contiguous sequences screened for open reading frames (ORFs) that may encode proteins involved in fiber-degradation. The large number of protein-encoding sequences containing dockerin modules detected indicates that R. flavefaciens FD-1 has the largest collection of cellulosome-associated proteins of any known fiber-degrading bacterium thus far described. Comparison with known enzymes from R. flavefaciens 17 indicates many subtle differences between the two strains in modular organization among enzymes involved in lignocellulose degradation. Additionally, gene expression profiling using microarray technology has allowed us to obtain functional information about the majority of the genome by comparing gene expression when R. flavefaciens FD-1 is grown on cellulose or cellobiose. These experiments have revealed that the substrate drives expression of the different enzymes involved in the degradation of cellulosic material, and suggests that the cellulosome plays a central role in this process.
Results and Discussion
Assessing functional coverage of the R. flavefaciens FD-1 draft genome
In combination with suppressive subtractive hybridization (SSH) sequences obtained from our previous comparative studies of R. flavefaciens FD-1 and JM1 , 430,226 sequence reads from GS FLX pyrosequencing and 28,681 ESTs from Sanger sequencing were assembled using the PHRED/PHRAP system , , , producing 119 primary contiguous sequences (Table 1). These contigs range in size from 205 bp (i.e. single unique reads) to 31,187 bp. A total of 4,339 ORFs were identified in R. flavefaciens FD-1. Of these, 2,289 (52.8%) could be assigned to biological role categories, 385 (8.9%) were conserved hypothetical proteins or conserved modular proteins, 422 (9.7%) were of unknown function, 79 (1.8%) were unclassified with no assigned role category, and 1,241 (28.6%) encoded hypothetical proteins. There appears to be one ribosomal operon harboring single copies of genes encoding the 16S and 23S rRNA molecules.
The total amount of unique sequence is 4,573,803 bp with an average GC content of 45%. This compares with an approximate estimate of 4.4 Mb genome based on pulsed-field gel estimates for the genome of the closely related strain, R. flavefaciens 17. According to the Poisson distribution, 29× coverage worth of genome sequencing should produce approximately 99.999% of the genome. An inventory of functional sequences was conducted based on TIGR's Annotation Engine output. It was decided to focus first on sequences related to amino acid biosynthesis for which we expected relatively conserved biosynthetic pathways. Previously, a gapped genomic approach was used to provide a functional analysis of amino acid metabolism of Thiobacillus ferrooxidans and to estimate the extent of genome coverage . Rumen bacteria, such as R. flavefaciens, have long been documented to require free ammonia in the medium, therefore the organism must be able to synthesize all of the necessary amino acids de novo , . MetaCyc was utilized to help visualize the metabolic steps for each of the amino acid families .
Of the 90 expected ORFs necessary for biosynthesis of the major families of amino acids in R. flavefaciens FD-1, 83 were detected suggesting that the overall genome size predicted by PFGE may be an underestimate. Nineteen ORFs encode enzymes involved in the biosynthesis of aromatic amino acids, and 19 ORFs encode enzymes involved in biosynthesis of the aspartate family of amino acids. In addition 23 ORFs were involved with the biosynthesis of the glutamate family, 19 ORFs with the pyruvate family, eight ORFs with the serine family and ten ORFs with the histidine family of amino acids (Table S1).
An inventory of full and partial ORFs revealed several sequences that matched those obtained previously by cloning and sequencing of individual genes. These include genes implicated in cellulose degradation (celA, celB and celD), as well as ammonia assimilation, and the heat shock (general stressor) response. This genome assembly has corrected for sequencing errors in the celB sequence currently in GenBank (gi|736356). Genome sequencing showed that the celB gene is actually 3471 bp, and has homology with the R. flavefaciens 17 family 44 cellulase. New features of the celB protein are a CBM, T-rich linker region and a dockerin domain. No sequence was detected for celE (gi|152634). Sequences matching the previously sequenced glutamate dehydrogenase (gdhA; gi|27461937) and glutamine synthetase type III (glnA; gi|2895903) were also represented in the draft sequence data: ORF01204 and ORF03347, respectively. Several ORFs that were identified match the cloned heat shock genes: ORF01108 and ORF02365 (for dnaK; gi|37779192) and ORF03100 and ORF03101 (for groESL; gi|37779196).
Cellulases and associated glycoside hydrolases
Based on comparison with the Carbohydrate Active Enzymes (CAZy) database (http://www.cazy.org) , sequences from the R. flavefaciens FD-1 genome were classified according to families and modules. Glycoside hydrolases, including those found in cellulases and hemicellulases (the latter referred to as xylanases and mannanases), have been organized into 114 families in the CAZy database. ORFs containing at least one predicted GH module can be seen in Table S2. The distribution of the 25 glycoside hydrolase families identified in R. flavefaciens FD-1 is dominated by families 5 and 9 (14 and 12 identified catalytic modules, respectively; Figure 1). These GH modules are characteristic of processive, endo-acting beta-1,4-glucanases. The repertoire of detected GH modules is summarized in Figure 1A and, in addition to families 5 and 9, includes representatives of families 2, 3, 10, 11, 13, 16, 18, 24, 25, 26, 31, 36, 42, 43, 44, 48, 53, 74, 77, 94, 95, 97, and 105. The presence of a GH family 48 module in ORF03925 is indicative of the presence of a processive exo-acting beta-1,4-glucanase. This ORF is also phylogenetically related to Cel48A from R. albus , which provides further evidence that this enzyme is a processive exo-acting enzyme (Figure S1; Table S3). A dockerin has also been detected in the same ORF supporting its integration into the R. flavefaciens FD-1 cellulosome.
Genes associated with the breakdown and utilization of xylans
One of the GH family 3 modules found in ORF02396 is homologous with the GH3 module from a β-xylosidase gene, which is included in a xylan utilization operon previously identified in R. flavefaciens 17 . This GH3 enzyme is presumed to function as a β-xylosidase and/or α-arabinofuranosidase, since these activities were associated with the cloned region . Homology extends downstream to include the gene for xylose isomerase (xsi), and three genes encoding components of an ABC transporter system (ugpA, B and E) (Figure 2). The gene encoding xylulokinase is located elsewhere in the FD-1 genome (ORF02846) whereas in most bacteria it is adjacent to the isomerase gene. ORF02390 encoding a dockerin-containing protein is found immediately downstream of the transporter genes in FD-1, while the gene for another dockerin-containing protein, XynD , is encoded by the region upstream of the GH3 xylosidase in R. flavefaciens 17 (Figure 2).
ORFs that include GH10 or GH11 xylanase modules commonly showed multiple catalytic modules. In one case, GH modules representing family 10 and 43 are detected in the same ORF (ORF03865; Table S2). One larger ORF (ORF03896; 4.5 kb) appears to encode a tetrafunctional endo-1,4-β-xylanase/acetyl xylan esterase, with a predicted molecular weight of 167,983 Da. The ORF contains several modules separated by glutamine-asparagine-rich linkers – two glycoside hydrolase 11 modules, a GH family 10 module, a CBM family 22 module, and a carbohydrate deacetylase at the C-terminal end. Additionally, a dockerin module is present indicating that it is cellulosome associated. This ORF was previously identified in the suppressive subtractive hybridization comparisons with R. flavefaciens JM1; . Southern blots had indicated that both the GH 10 and 11 modules appeared in at least two separate EcoRI restriction fragments, and support the modular arrangement described in Table S2. A comparison of the modular organization inferred for xylanolytic enzymes from R. flavefaciens strains FD-1 and 17 is shown in Figure 3, which shows that while similar features are present, no two modular arrangements are identical between the two strains. The non-cellulosomal (ie. non dockerin-containing) enzyme XynA from R. flavefaciens 17 was previously reported to include a large NQ-rich linker, interconnecting GH11 and GH10 modules . Although T-rich linkers are predominant in glycoside hydrolases from FD-1, three gene products were detected that carry NQ-rich linkers, or in one case a mixture of T-rich and NQ-rich linkers (Figure 4). The average amino acid composition of the five linkers within FD-1-ORF03896 (33% N, 35% Q, 10% W) was quite similar to that of the single large linker in R. flavefaciens 17 XynA (45% N, 26% Q, 16% W) . The presence of the aromatic residue tryptophan in such linker regions is particularly unusual.
Catalytic modules are indicated by glycoside hydrolase enzyme family (GH10, GH11, CE3 etc). Families of carbohydrate binding modules (CBM22 etc) and dockerin modules (Doc) are also indicated. All complete ORFs carry a predicted signal peptide at the N terminus (not shown). Incomplete ORFs are indicated by an asterisk.
Permutations of glycoside hydrolases and carbohydrate-binding modules that occur in R. flavefaciens FD-1 are displayed in Table S4. The presence of CBMs in tandem with catalytic modules provides prolonged association with the substrate and can be found at either the N- or C-terminus of fiber-degrading enzymes. They are usually separated from the catalytic module by linker segments that are rich in proline, threonine and serine residues . Over half of the identified CBMs in R. flavefaciens FD-1 are family 22 and 35 (Figure 1). Members of CBM families 3, 4, 6, 13, 32, and 48 were also identified. Additionally, there were 5 putative CBM modules that are presently unclassified in CAZy. The five CBM family 3 modules in the R. flavefaciens FD-1 genome were all found in tandem with a GH9 module. All five CBM3 modules fell within the CBM3c subfamily when compared to CBM3 modules from other organisms (Figure S2; Table S5). When paired with a particular subfamily of GH9, the CBM3c subfamily is thought to contribute in some cases to the property of processivity, allowing the enzyme to exhibit both endo- and exoglucanase activities , , . The fact that none of the CBM3s map into subfamilies 3a or 3b indicates that none of them fulfill a defined binding capacity for crystalline cellulose. In ten ORFs, multiple CBMs are detected (ORF01222, ORF01406, ORF1541, ORF02983, ORF3116, ORF03219, ORF3447, ORF03865, ORF4012, and ORF04293). Of the 52 GHs found in tandem with CBMs, eight are of the GH43 family and all eight are encoded in tandem with dockerins. The majority of these encode arabinofuranosidases and arabinases. A close homologue was also found in ORF01571-ORF01570 for the new CBM family of cellulose-binding module that was identified adjacent to the GH44 catalytic module of R. flavefaciens 17 EndB (Cel44A) enzyme . Another suspected new CBM is present in the EndA cellulase of R. flavefaciens 17  and again a close homologue was detected in R. flavefaciens FD1 (ORF01388). Homologues (ORF03116) were also detected for the two new CBMs recently detected in the cell wall-attached, non-catalytic, dockerin-containing protein CttA that is encoded by the sca gene cluster .
Phylogenetic relationships of GH5 and GH9 catalytic modules
The hypothetical translations representing the most prevalent glycoside hydrolases (GHs) detected (families 5 and 9) were aligned with other GH representatives from a variety of other fiber-degrading organisms using ClustalX . The neighbor-joining tree produced from the GH family 5 alignment demonstrates an interesting phenomenon with relation to repeated modules within the same ORF (Figure S3; Table S6). Most known GH5 enzymes show cellulase activities, although numerous members of this family display xylanase and mannanase activities. In the GH family 5 phylogeny the two modules from ORF01388 appear less related to each other relative to the other representatives. The N-terminal module (ORF01388a) appears more closely related to the GH family 5 module detected in ORF00389 and ORF02868 and map together with known endoglucanases from R. albus and R. flavefaciens strain 17, whereas the C-terminal module from ORF01388 (ORF01388b) appears more closely related to the module detected in ORF00227, both of which are predicted mannanases. ORF03338 and ORF04165 map on a branch together with known xylanases.
As indicated in the previous section, five of the twelve GH family 9 modules, contained in ORF01045, ORF01053, ORF01132, ORF02970, and ORF02981, appear in tandem with CBM subfamily 3c modules. In these five processive endoglucanases, the family 3c CBMs appear adjacent to the GH family 9 module, towards the C-terminal end of the polypeptide (Table S2). The five GH9-CBM3c enzymes present one of the major thematic architectural schemes, which characterize this family of cellulases. The five GH9 catalytic modules map on one of the major branches of the phylogenetic tree (Figure S4; Table S7), together with two other GH9 modules (ORF01327 and ORF01899), each of which bears a module currently annotated as an unknown module in place of the CBM3c. It will be interesting in the future to determine whether this type of unknown module functions as a CBM and modulates the activity characteristics of the GH9 catalytic module. The remaining five family GH9 enzymes of R. flavefaciens FD-1 map on the phylogenetic tree on the second major branch together with GH9 enzymes of other bacterial species that include a family 4 CBM (Figure S4; Table S7). Indeed, all five of the latter enzymes bear an N-terminal CBM4, in accord with a second major thematic architectural scheme of the GH9 enzymes.
In contrast to the situation with polypeptides that carry GH10 and GH11 xylanase modules (Figure 3), there were rather few instances where GH5 or GH9 modules were combined with other catalytic modules in the same polypeptide. Thus for the six completed ORFs that include a GH5 module, and the four completed ORFs that include a GH9 module, these were the only identified catalytic module present, as opposed to some examples of multiple catalytic modules that occur in GH9 and GH5 enzymes of the Clostridium thermocellum cellulosome. Among incomplete ORFs, however, one (ORF01388) showed evidence of two GH5 modules of divergent specificities.
Presence of cellulosome components in R. flavefaciens FD-1 – scaffoldins and complementary cohesin and dockerin modules
Scaffoldin sequences have been previously described and characterized for R. flavefaciens 17 , , , . Using this sequence information, FastA searches of the R. flavefaciens FD-1 genome sequence were initially conducted in order to determine what components are maintained between R. flavefaciens strains, particularly components crucial to cellulosome formation. This led to the subsequent sequence and functional analyses between the scaffoldins of strains 17 and FD-1 described recently . These studies showed a general similarity in cellulosome organization between the strains, including homologs of ScaA, ScaB, ScaC, and ScaE (see Table S8). However, the studies also revealed that ScaB from the FD-1 strain is comprised of two divergent cohesin types, unlike ScaB from strain 17, which is comprised of a single cohesin type. This description of scaffoldins in R. flavefaciens complements the previous identification of dockerin-like modules in both R. flavefaciens and R. albus , , , . The presence of dockerin-containing proteins in R. flavefaciens FD-1 was expected, given the presence of cohesin-carrying scaffoldins. According to our analyses, the genome appears to encode for 225 dockerin-containing proteins (including those found in the aforementioned scaffoldins). The dockerins are found within almost all of the glycoside hydrolase-containing ORFs (Figure 1A and Table S2). Signal peptides were detected in all completed ORFs that include a dockerin, thus indicating secretion of these proteins (Table S8).
Presence of non-carbohydrate active enzyme dockerin-containing ORFs
Analysis of the cellulosome associated ORFs revealed an astonishing number of non-carbohydrate acting enzymes linked to dockerins that made up 21% of the cellulosome associated ORFs. These ORFs include such modules as leucine rich repeats (LRR), transglutaminases, and serine protease inhibitors (SERPIN). Although these modules may not have a direct role in plant cell wall degradation, they could play a role in cell adhesion and protein-protein interactions. The LRR modules in particular have been shown to form protein-protein interactions , and thus they could act as a new type of cohesin.
Comparing abundance of carbohydrate active enzymes among cellulolytic bacteria and the rumen metagenome
A recent study by Brulc et al.  sequenced the metagenome of the rumen of three steers, and looked specifically for carbohydrate active enzyme (CAZy) families in both the planktonic and fiber-adherent fractions of the rumen contents. The results of this study showed a large variety and abundance of GH families, most of which can also be found within the genomes of R. flavefaciens FD-1 and C. thermocellum (Table 2a). The most abundant GH families in both R. flavefaciens FD-1 and C. thermocellum are the GH families 5 and 9, whereas in the rumen metagenome the GH families 2 and 3 had the highest number of copies detected. The most likely reason for this is due to the fact that both R. flavefaciens and C. thermocellum specialize in crystalline cellulose degradation and thus two of the cellulase families are seen in the highest abundance, whereas in the rumen environment the population of cellulolytic bacteria is low compared to the overall microbial population and thus we see comparatively few cellulases detected. Alternatively, there may be difficulties in releasing of DNA from ruminococci as they are Gram positive and are in tight association with insoluble substrate. In the C. thermocellum and R. flavefaciens FD-1 genomes there are also many types of CBMs, though few were detected in the rumen metagenome (Table 3). The most abundant CBMs in the R. flavefaciens FD-1 genome were from family 22 (19 copies), and in the C. thermocellum genome the most abundant CBMs were from family 3 (23 copies). The total number of carbohydrate esterases (CE) detected in the rumen were comparable to the numbers seen in the R. flavefaciens and C. thermocellum genomes (Table 3). A single polysaccharide lyase (PL) was detected in the rumen samples, but the number of PLs compared to other carbohydrate active enzyme types was also rather low in both genomes (Table 3). The feature unique to R. flavefaciens FD-1, however, is the large copy number of dockerin sequences (225) compared to C. thermocellum (76 copies). Surprisingly, a mere 3 copies of dockerin modules were detected in the rumen metagenome (Table 3), which is most likely due to the rarity of cellulosome-based systems for plant cell wall degradation within the rumen community and the limits of the short pyrosequencing read lengths, as described by Brulc et al . None of the dockerin modules from the rumen metagenome were consistent with those of R. flavefaciens FD-1.
Microarray gene expression profiling upon growth of R. flavefaciens FD-1 on cellulose or cellobiose
A clone-based cDNA microarray was created by amplifying clone inserts from the most recent library used in the sequencing of the R. flavefaciens FD-1 genome to compare gene expression when R. flavefaciens FD-1 was grown on cellulose or cellobiose as a carbon and energy substrate. Clone sequences encoding ORFs believed to be associated with the cellulosome or involved in degradation of polysaccharides, were identified by BLAST searches of a local database and by the genome annotation of R. flavefaciens FD-1, which was provided by TIGR's Manatee annotation engine. Normalized signal ratios for each spot corresponding to ORFs involved in polysaccharide degradation were calculated representing gene expression for cells grown on cellulose compared to those grown on cellobiose. Clones with an FDR-adjusted p-value less than 0.5 were considered significant. A transcript was considered to be up-regulated if the average of the signal ratio for the ORF was 2-fold or greater, and considered down-regulated if the average of the signal ratio was 0.5-fold or less. The expression of any gene transcript falling below 2-fold and above 0.5-fold was considered to be unaffected by the substrate .
Cellulosome-associated ORFs included any ORF that encoded a dockerin module. As reported above, the draft genome of R. flavefaciens FD-1 encodes 225 predicted dockerin modules. These ORFs, the number of clones in each ORF that was included on the microarray, and the corresponding average signal ratios can be seen in Table S9. Of these 225 cellulosome-associated ORFs: 56 were up-regulated, 14 were down-regulated, 135 were unaffected, and 20 were not represented on the microarray. The 20 dockerin-containing ORFs not represented on the microarray due to the inclusion of only 2× coverage of the genome on the microarrays included numerous additional modules and/or domains: 15 ORFs contain domains of unknown function, the remaining five ORFs contain a serpin, a leucine-rich (LRR) domain, a CBM4-GH9, a CBM35-GH26, and a GH18.
The Sca cluster in R. flavefaciens FD-1, which includes the main scaffoldins: ScaA, ScaB and ScaC, was significantly up-regulated en bloc approximately 4.5 fold, which suggests that these genes are co-expressed either as a polycistronic mRNA or sharing the same regulator with similar affinity for these genes. The last two genes of the Sca cluster, cttA and ScaE, do not appear to be co-expressed with ScaA, ScaB, and ScaC, and appear to have different regulators. The last scaffoldin gene of the cluster – the putative cellulosome anchoring scaffoldin, ScaE, had significant relative expression of 2.94. The product of the linked gene cttA, exhibited a relative expression of 0.75 fold and thus appeared to be unaffected by the substrate. Fold changes for ScaA, ScaB, ScaC, and ScaE can be seen in Table S9. Of the other putative scaffoldins, ORF00794, ORF04069, and ORF04333 were unaffected, ORF03129 appears to be down-regulated (0.47 fold) and ORF01453 was significantly up-regulated 4.27 fold.
Results for some other genes were of particular interest. ORF01132 contains a family-9 processive endoglucanase, which has been described as an important cellulosome component of other species of bacteria , , . This processive endoglucanase was up-regulated 4.49 fold. CelA (ORF00507) and CelD (ORF01899) were unaffected (1.10 fold) and up-regulated (4.93 fold) respectively (Table S10), which is consistent with previous results , . CelB (ORF01869) was unaffected (1.13 fold; Table S9), which contradicts previous data that indicated that it was inducible by cellulose , , . A putative exo-acting GH48 of the R. flavefaciens FD-1 genome (ORF03925) was unaffected by the substrate, unlike the observed up-regulation of the C. thermocellum cellulosomal GH48 , , . These apparently different expression patterns are likely due to the different environmental conditions to which these two bacteria are exposed, including oxygen concentrations and plant cell wall substrate type.
The proportion of cellulases compared to enzymes cleaving non-cellulosic plant cell wall polysaccharides and other ORFs within the cellulosome-associated ORFs that encode a dockerin module is shown in Figure 5. Cellulases (GH families 5, 8, 9, and 48) made up 25% of the up-regulated ORFs compared to 10% of all dockerin-encoding ORFs. The enzymes cleaving non-cellulosic plant cell wall polysaccharides made up 23% of all dockerin-encoding ORFs and 34% of the up-regulated cellulosomal ORFs. Enzymes cleaving non-cellulosic plant cell wall polysaccharides also accounted for some of the highest relative expression when grown on cellulose. The three cellulosome-associated ORFs with the highest regulation were the multi-modular xylanases: SIGN-GH11-CBM22-GH10-DOC-CBM22-CE4 (ORF01222), SIGN-GH11-CBM22-GH10-DOC1-GH11-CE4 (ORF03896) and SIGN-GH11-CBM22-DOC-GH11-CE3 (ORF01315) with respective significant relative expression levels of approximately 63, 50, and 25 fold above those of cellobiose-grown cells. The predicted ORF03896 product is one of the ORFs containing NQ-rich, rather than T-rich linker sequences. Such linkers have been reported previously in only one non-cellulosomal xylanase from R. flavefaciens 17 that also included GH11 and GH10 catalytic modules .
Up-regulated genes are those dockerin-containing ORFs that have fold changes of 2-fold or greater when grown on cellulose. For the purposes of this work, the putative cellulases include any ORF containing glycoside hydrolase (GH) families 5, 8, 9, and 48. The enzymes cleaving non-cellulosic plant cell wall polysaccharides (mainly hemicellulases) include ORFs containing GH families 10, 11, 16, 26, 43, 44, 53, 74 105, some subfamilies of GH5, all families of polysaccharide lysases (PL) and carbohydrate esterases (CE). ORFs that did not have any significant hits in the database are grouped as “unknown,” and ORFs that do not fall into any of the previous categories are grouped as “other.” Putative β-glucosidases and β-xylosidases were ORFs containing sequences consistent with GH family 3.
Non-cellulosomal open reading frames, i.e. those ORFs that do not contain a dockerin module, are listed in Table S10. Of the 71 genes included in this list, 4 (6%) were up-regulated, 6 (8%) were down-regulated, 54 (76%) were unaffected, and 7 (10%) were not included on the microarray. The genes that are not on the microarray are composed of five GH family 25 modules (two of which are found in a single ORF), a GH family 3 module, a CBM family 22 module, and a glycosyltransferase family 28 module.
Comparison of relative gene expression using quantitative real-time reverse transcriptase PCR
RNA samples that were extracted from cellulose- and cellobiose-grown cultures of R. flavefaciens FD-1 were used for quantitative real-time reverse transcriptase PCR (qRT-PCR), in order to validate the microarray data. The same RNA samples that were used for the microarray experiments were used for these qRT-PCR experiments. Five genes of particular interest to us were selected based on their putative function and/or dramatic change in relative gene expression between the two conditions. These genes include: a multi-modular xylanase (ORF03896), a GH family 9 processive endoglucanase (ORF01132), a GH family 48 exoglucanase (ORF03925), ScaA (ORF03114), and a highly down-regulated dockerin-containing gene of unknown function (ORF04112). The primer sequences for these genes and the normalization gene, gyrA, are listed in Table S11. The gene, gyrA, was chosen as a reference gene to normalize the qRT-PCR data because it did not have a statistically significant change in expression, based on the results of the microarray experiments, and it has been commonly used as a normalization gene for bacteria in other studies , , , . The 16S gene was also intended for use as a normalization gene, but was found to produce inconsistent results with these samples (data not shown). A relative standard curve method was used to determine the relative expression of these genes (Applied Biosystems User Bulletin 2; ). Serial dilutions of R. flavefaciens FD-1 genomic DNA were used to generate standard curves to determine the relative copy numbers of the cDNA samples by correlating the samples to particular concentration.
The qPCR data confirmed the up-regulation of three ORFs, and the down-regulation of one, although the magnitude of the regulatory changes was greater than in the microarray study (Table S12, Figure 6). In the case of the GH48 enzyme encoded by ORF03925, up-regulation was detected by qPCR but not by microarray. The difference between the qPCR and microarray data for ORF03925 could be due to decreased sensitivity of the microarray or could be explained by a low correlation between microarray and qPCR results in genes that exhibit low changes in expression between treatments . The qPCR results, which indicate up-regulation of the GH48 enzyme, are more in accord with the previously reported data for the orthologous C. thermocellum enzyme , , .
Portions of the cellulolytic enzyme system from R. flavefaciens strain FD-1 have been previously characterized as a variety of exo-β-1,4-glucanases, endo-β-1,4-glucanases, and cellodextrinases , , , . Evidence was found for two major endo-β-1,4-glucanase complexes, one including at least 13, and the other at least 5, electrophoretically separable endo-β-1,4-glucanase activities . This is consistent with the large diversity of genes found here that have the potential to encode endoglucanase activity.
Complex multi-modular organization, involving multiple catalytic and substrate-binding modules within the same polypeptide, has been documented previously for plant cell wall degrading enzymes, especially xylanases, from the related strain R. flavefaciens 17 , , , . This genomic analysis establishes that such organization is a common feature in particular of xylanases and esterases from R. flavefaciens FD-1. Interestingly, however, despite many close similarities and common features, it was not always possible to identify precise homologues of these multi-modular enzymes between the two strains. Of the five xylanases and esterases characterized from R. flavefaciens 17, for example, none showed an exact match in modular structure to a homologue in strain FD-1. R. flavefaciens FD-1 ORF02390, for example, shares close homology with R. flavefaciens 17 CesA (CE3B) through its family 3 esterase and an unknown domain, at the N and C terminus respectively, but includes an additional CBM22 module. R. flavefaciens FD-1 ORF03896 and R. flavefaciens 17 XynA are superficially similar in carrying GH11 and GH10 xylanase modules and NQ-rich linkers, but the FD-1 ‘superzyme’ differs in carrying additional CE4 and GH11 modules and a dockerin. This suggests that there is considerable evolutionary plasticity in the modular structures of these enzymes, with domain shuffling occuring readily to produce new variations within a given strain . Close homologues were, however, observed for certain enzymes, such as R. flavefaciens 17 EndB (Cel44A).
Close similarities in gene order between R. flavefaciens FD-1 and 17 were identified for two important chromosomal regions concerned with the utilization of plant cell wall polysaccharides. Conservation of the four key cellulosomal scaffoldin genes within the sca cluster, scaC, scaA, scaB, and scaE was reported recently . An additional gene cttA, found within the cluster whose product is concerned with cell adhesion to cellulose  was also conserved. The microarray results also showed that when grown on cellulose, scaA, scaB, and scaC in R. flavefaciens FD-1 all have similar signal ratios (approximately 4.5 fold above that of cellobiose) implying that they are transcribed together, forming an operon. Compared to R. flavefaciens 17, however, differences were observed at the level of modular organization with the R. flavefaciens FD-1 ScaA protein carrying one fewer cohesin module than ScaA from R. flavefaciens 17, and with the FD-1 ScaB protein exhibiting two types of cohesin . Along with the frequent differences in enzyme modular structures noted above, this suggests that there may be many differences in the detailed organization of the cellulolytic enzyme complexes between the two strains. We were also able to demonstrate a region of synteny between genes concerned with the utilization of xylo-oligosaccharides  that include the β-xylosidase, xylose isomerase and components of an ABC transporter system. In both of the strains, this region was found to be flanked by genes that encode cellulosomal enzymes associated with the degradation of hemicellulose.
The variety of dockerin-containing enzymes in the R. flavefaciens FD-1 genome suggests that there are many configurations that the cellulosome can assume. Expression profiling using microarrays, and verified by qRT-PCR, revealed that the type of substrate utilized by R. flavefaciens FD-1 drives the potential cellulosome composition. This is expected to result in the production of an incredibly heterogeneous collection of cellulosomes during the course of plant cell wall polysaccharide degradation. It is interesting to note that the minority (33%) of the 225 dockerin containing ORFs was made up of the cellulases and enzymes active against non-cellulosic structural polysaccharides (Figure 5). However, when looking exclusively at the up-regulated dockerin-containing ORFs, the cellulases and enzymes active against non-cellulosic structural polysaccharides made up 59% of the ORFs. This indicates that when grown on a cellulose substrate, R. flavefaciens FD-1 preferentially expresses enzymes that are designed for hydrolysis of complex carbohydrates. Curiously, of these ORFs, the most highly up-regulated enzymes during growth on cellulose were the hemicellulases, not the cellulases. The three most highly up-regulated enzymes show remarkably complex structures, each with three catalytic modules and one or more CBMs. Interestingly, previous studies on R. flavefaciens 17 showed by zymogram analysis that high molecular weight xylanase polypeptides (>70 kDa) were expressed during growth on cellulose, or in some cases only on xylan or oat straw, but not on cellobiose . A likely explanation for these findings is that, in nature, R. flavefaciens rarely comes across pure cellulose, because cellulose is typically accompanied by other plant cell wall polysaccharides. Therefore, in order to depolymerize these other non-cellulosic components and gain access to the cellulose, the microbe would need to use enzymes other than the cellulases to remove the non-cellulosic plant cell wall components. In addition, many R. flavefaciens strains are able to utilize products from xylan, as well as cellulose breakdown, for growth .
Materials and Methods
Organisms and culture conditions
R. flavefaciens FD-1 from the Department of Animal Sciences culture collection was used as the source of genomic DNA in library construction and was cultivated in a defined medium as described by Antonopoulos et al . Cells were grown at 37°C in crimped butyl rubber stoppered bottles (Bellco Glass, Inc., Vineland, NJ) saturated with 95% CO2/5% H2 atmosphere. Stock cultures were maintained on solid agar slants at −120°C. Escherichia coli One Shot® MAX Efficiency® DH10B™ (Invitrogen, Carlsbad, CA) was used as the host in library constructions. Transformed E. coli cells were grown in LB medium supplemented with 100 µg/mL of ampicillin (Sigma-Aldrich, St. Louis, MO) for selection and maintenance of plasmids.
Genomic DNA extraction and shotgun library construction
Extraction of genomic DNA from R. flavefaciens FD-1 has been described previously . Chromosomal DNA extracted from R. flavefaciens FD-1 was subjected to high-pressure shearing (N2) via a nebulizer and then treated with Bal31 nuclease (New England Biolabs, Beverly, MA) to remove single-strand overhangs. This pool of sheared DNA fragments was then size fractionated (i.e. fragments between 1.5–3 kb were gel excised), gel purified, and subjected to a series of “polishing” reactions by T4 DNA polymerase and Klenow fragment (Invitrogen, Carlsbad, CA). Shrimp alkaline phosphatase was then used in a dephosphorylation treatment to remove 5′-phosphoryl groups (Roche Applied Science, Penzberg, Germany). Cloning of the sheared, “polished”, and dephosphorylated fragments was performed using the pCR®4Blunt-TOPO® vector (Invitrogen, Carlsbad, CA). Transformation of Escherichia coli One Shot® MAX Efficiency® DH10B™ cells (Invitrogen, Carlsbad, CA) was conducted by electroporation (transformation efficiency of 107 transformants/µg DNA) followed by immediate plating onto ampicillin-supplemented LB agar plates.
Sequencing and assembly of contigs
In total 11,520 transformants were picked robotically using a QPix robot (Genetix, UK) and transferred into starter freeze-down media in 384-well plates. Following overnight incubation the plates were transferred to a −80°C freezer for storage. To sequence the selected clones, they were transferred from the frozen stocks to 96-well plates, grown overnight, and a QIAGEN 9600 robotic system was then used to extract the plasmids. Big Dye terminator chemistry, in conjunction with standard M13-based forward and reverse primers based on the pCR®4Blunt-TOPO® vector, was used for the sequencing reactions on an ABI 3700 capillary system (conducted at the W. M. Keck Center for Comparative and Functional Genomics on the UIUC campus). In addition to Sanger sequencing, extracted genomic DNA was subjected to a pyrosequencing run on a Roche 454 GX-FLX system at the W. M. Keck Center for Comparative and Functional Genomics on the UIUC campus. Vector trimming, sequence editing, and quality control was handled by the Bioinformatics Unit of the W.M. Keck Center, as well as the maintenance of the sequence database on their servers. Base calling and contig assembly were conducted using Phred/Phrap and visualized with Consed , , . Subsequent, manual linking of contigs was performed using Consed . The genome sequence (119 contigs) has been deposited into the DDBJ/EMBL/GenBank databases under the accession number ACOK00000000.
ORF identification and annotation
Following contig assembly and vector trimming, the contigs comprising the assembly were used in BlastX comparisons with the locally stored non-redundant GenBank database (a cut-off E-value of e−05 was used for the initial survey; ). These preliminary sequence identifications were supplemented by focused searches of the assembly using the FastA collection of programs and individual sequences of interest as queries . Identification and annotation of putative genes from the R. flavefaciens FD-1 sequence assembly was also performed by TIGR's Annotation Engine (a service funded by the US Department of Energy; see TIGR's Annotation Engine website for further details at http://www.tigr.org/edutrain/training/annotation_engine.shtml). The Glimmer software package was used initially to identify likely candidates for genes , . Several searches were then performed using the candidate ORFs identified by Glimmer as queries. BLAST-Extend-Repraze (BER) was used to search TIGR's non-redundant amino acid database (nraa) containing all proteins available from GenBank, PIR, SWISS-PROT, and TIGR's Comprehensive Microbial Resource (CMR) database . A second round of searches were performed against hidden Markov models using the hmmpfam program . AutoAnnotate was then used to analyze the BER and HMM searches and to assign a function to each of the sequences.
Organisms and growth conditions for microarrays
Ruminococcus flavefaciens FD-1  was grown anaerobically in defined media containing either 0.1% w/v pebble milled cellulose (filter paper) or 0.4% w/v cellobiose, 0.2% w/v Bacto-Tryptone, 0.1% w/v Bacto-Yeast Extract, 5% v/v mineral solution 1 and 2 , 1% v/v volatile fatty acid (VFA) solution , 0.0001% w/v resazurin, 0.4% w/v NaHCO3 and 0.025% w/v cysteine-sulfide. Cultures were grown at 37°C in butyl rubber-stoppered flasks under a 95% CO2/5% H2 atmosphere. Growth curves were determined for both cellobiose and cellulose cultures by measuring optical density at 600 nm for cellobiose and by monitoring substrate disappearance for cellulose (data not shown). Growth curve data were also compared to growth curves performed by Odenyo et al. (1992; 1994). Media containing cellobiose was grown for approximately 9 h late to log phase . Media containing cellulose was grown for approximately 19 h to late log phase (Odenyo, 1992 PhD thesis, University of Illinois at Urbana-Champaign). Four independent replicate cultures were grown in triplicate for each substrate.
Cells were pelleted for RNA extraction by first adding 75 ml of ice-cold RNase free DEPC-treated water per 50 ml of cell culture, placing on ice for 5 min, then centrifuging at 4°C for 5 min at 2,800 x g. Supernatant fluids were removed, and cells were resuspended in 2.5 ml ice-cold RNase free DEPC-treated water. One ml aliquots of the cell suspension were transferred to 2.0 ml screw cap tubes and centrifuged at room temperature for 15 s at 13,000 x g. Supernatant fluids were removed, and cell pellets were stored at −20°C until needed for RNA extraction. RNA was extracted from the cell pellets using the RNeasy Kit – Yeast III Protocol (Qiagen, Valencia, CA) according to the manufacturer's instructions. A mini-bead beater set to homogenize was used as part of the lysis process. Cells were homogenized in the mini-bead beater 3 times for 2 min each and cooled on ice for 2 min between each homogenization. A DNase digestion was carried out using the On-column DNase Digestion with RNase-free DNase Set (Qiagen, Valencia, CA) according to the manufacturer's instructions. RNA quality was assessed by 1% agarose gel electrophoresis after treatment with an equivalent volume of 10 M urea and heating at 70°C for 5 min to eliminate any secondary structure. RNA concentrations were estimated by absorbance at 260 nm using a Beckman DU-7000 spectrophotometer.
Microarray design and construction
6,144 PCR-amplified clone inserts from the RF03 library were spotted in duplicate onto slides at the W.M Keck Center for Comparative and Functional Genomics, using a Gene Machines OmniGrid 100 Microarrayer (Genomic Solutions, Ann Arbor, MI). Controls consisted of R. flavefaciens FD-1 genomic DNA, R. flavefaciens FD-1 16S V3 rDNA tag, E. coli genomic DNA, E. coli 16S V3 rDNA tag, and a no template control consisting of the buffer only. The RF03 library is the most recent clone library to be included into the R. flavefaciens FD-1 draft genome, and therefore the microarrays contain the R. flavefaciens FD-1 genome at approximately 2× coverage (Antonopoulos 2004 PhD dissertation, University of Illinois at Urbana-Champaign).
Aminoallyl-labeling of RNA
RNA was labeled by reverse transcription as follows: 5 µg of RNA was mixed with 2 µl of random hexamer primers (3 mg/ml) (Invitrogen, Carlsbad, CA) in a final volume of 18.5 µl, and incubated at 70°C for 10 min then placed on ice. The labeling reaction (0.5 µl of RNase inhibitor (Invitrogen, Carlsbad, CA), 6 µl of 5× First Strand Buffer (Invitrogen, Carlsbad, CA), 3 µl of 0.1 M DTT (Invitrogen, Carlsbad, CA), 0.6 µl 50× aminoallyl-dNTP mix [25 mM dATP, 25 mM dCTP, 25 mM dGTP, 5 mM dTTP, 7 mM aa-dUTP], and 2 µl SuperScript III RT (200 U/∝L) [Invitrogen, Carlsbad, CA]) was added to the mixture and the reaction was incubated at 46°C overnight. RNA was hydrolyzed by addition of 10 µl each of 1 M NaOH and 0.5 M EDTA, followed by incubation at 65°C for 15 min. The reaction was neutralized by addition of 10 µl of 1 M HCl. Ten µl of 3 M Na•Acetate, pH 5.2 was added to facilitate binding of cDNA to the Qiagen column. Unincorporated aa-dUTP and free amines were removed using a Qiagen QIAquick PCR Purification Kit protocol, according to manufacturer's instructions, and substituting phosphate wash buffer [5 mM KPO4 pH 8.5, 80% EtOH] for Buffer PE and phosphate elution buffer [4 mM KPO4, pH 8.5] for Buffer EB. The elution step was carried out twice with 30 µl of phosphate elution buffer.
Purified cDNA samples were dried in an Eppendorf Vacufuge for approximately 1 h. Alexa-fluor 555 and 647 (Invitrogen, Carlsbad, CA), which correspond to Cy3 and Cy5 respectively, were resuspended in 9 µl of 0.1 M Na2CO3 then transferred to the cDNA sample and mixed. The reactions were incubated in the dark for 1 h at room temperature. Uncoupled dyes were removed using the Qiagen PCR Purification Kit according to the manufacturer's instructions. The elution step was carried out twice with 40 µl of Buffer EB. The labeling reactions were analyzed by measuring absorbance at 260 nm and either 550 nm (Alexa-555) or 650 nm (Alexa-647). The amount in pmol of cDNA, incorporated Alexa-555 and Alexa-647 dyes were calculated, and a cDNA/dye ratio was determined for each sample; greater than 30 pmol of dye incorporation and a ratio less than 50 nucleotides/dye molecule is optimal. Samples were dried to completion in an Eppendorf Vacufuge.
Microarray hybridization, washing and scanning
The microarrays were prepared for hybridization by first binding the DNA to the slides by rehydrating the microarray over steam, drying on a heat block (∼70°C), then placing in a UV stratalinker at 300 mJ. Microarrays were washed vigourously in 0.2% SDS (w/v), then twice in ddH2O for two minutes each. The microarrays were added to preheated (42°C) pre-hybridization buffer [20% Formamide (v/v), 5× Denhardt's, 6× SSC, 0.1% SDS (w/v), 25 µg/ml tRNA] and incubated at 42°C for 45 min, shaking occasionally. Microarrays were washed in ddH2O five times, once in isopropanol, then dried immediately by centrifugation.
Hybridization was performed with four biological replicates, which included a dye swap. Labeled cDNA was resuspended in 80 µl of preheated (68°C for 15 minutes) SlideHyb #1 (Ambion). Samples were heated at 95°C for 5 min, then all 80 µl was applied to the microarray slide. 10 µl of ddH2O was added to the hydration chambers of the waterproof Corning hybridization chamber (Corning Life Science) to ensure a humid environment. Microarrays were allowed to hybridize in a 42°C water bath in the dark for approximately 3 d.
Coverslips were removed after hybridization and the microarrays were washed in 1× SSC, 0.2% w/v SDS at 42°C, followed by 0.1× SSC, 0.2% w/v SDS at room temperature, and twice in 0.1× SSC at room temperature agitating for 5 min at each step. The microarrays were dried immediately by centrifugation and scanned using an Axon GenePix 4000B scanner (Molecular Devices).
Slide images were analyzed using the spot finding feature of GenePix Pro 6.0 (Molecular Devices). Microarrays were manually edited and aberrant spots were flagged for exclusion later on in the analysis. The resulting files were loaded into GeneSpring GX 7.3 (Agilent Technologies). The microarrays were normalized using Lowess normalization and the t-test p-values were FDR adjusted such that spots with an FDR p-value of less than 0.05 were considered significant. Each spot, which corresponds to a sequence from the genome assembly, was mapped back to the most current R. flavefaciens FD-1 genome assembly. Fold changes of dockerin-containing ORFs and glycoside hydrolase-containing ORFs were analyzed using Microsoft Excel to calculate the average of the signal ratios. Fold changes greater than or equal to 2-fold were considered up-regulated and fold changes less than or equal to 0.5-fold were considered down-regulated . Microarray data were submitted to the Gene Expression Omnibus (GEO) in accordance with MIAME standards under GEO accession number GSE15916.
Quantitative real time RT-PCR
qRT-PCR was performed to confirm the gene expression results of the microarrays. Aliquots of 0.5 µg of RNA were converted to cDNA via the SuperScript III First Strand Synthesis SuperMix for qRT-PCR (Invitrogen, Carlsbad, CA) according to the manufacturer's instuctions. Each qPCR reaction consisted of 1× SYBR Green Master Mix (Applied Biosystems, Foster City, CA), 50 nM of forward primer, and 50 nM of reverse primer to which 1 µl of undiluted cDNA was added. All reactions were done in triplicate. Primers were designed for ORFs 1132, 3114, 3896, 3925, and 4112, as well as for gyrA (ORF02752), using Primer3 (http://workbench.sdsc.edu/) and synthesized by Sigma-Genosys (Table S11). The reactions were run on an ABI 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA). The cycling conditions consisted of a hold at 50°C for 2 min, a hold at 95°C for 10 min, 40 cycles of 95°C for 15 s and 60°C for 1 min, and then a dissociation profile of 95°C for 15 s, 60°C for 15 s, and 95°C for 15 s. The relative standard curve method was used to determine the relative amount of gene expression in R. flavefaciens FD-1 when grown on cellulose or cellobiose. R. flavefaciens FD-1 genomic DNA was serially diluted in TE Buffer, pH 8.0 from 10−1 to 10−6 to be used as standards for the standard curves from which the quantities of cDNA in the samples were determined. The gyrA gene (ORF02752) was used to normalize the Ct values from each sample prior to comparison , .
Unrooted dendrogram of the putative glycoside hydrolase family 48 modules (pfam02011) detected in R. flavefaciens FD-1 compared with those of other organisms.
(0.70 MB TIF)
Unrooted dendrogram of putative family 3 carbohydrate-binding modules detected in R. flavefaciens FD-1 compared with those from other organisms. “RfFD-1” refers to R. flavefaciens FD-1, and is followed by ORF designation number assigned by TIGR's Annotation Engine. “Clotm” refers to C. thermocellum, “Rumal” refers to R. albus, and these are followed by the enzyme name.
(0.80 MB TIF)
Unrooted dendrogram of glycoside hydrolase family 5 modules detected in R. flavefaciens FD-1 compared with those from other organisms. “Rf” refers to R. flavefaciens, and the ORF number refers to TIGR's Annotation Engine designation. The scale bar indicates the percentage (0.1) of amino acid substitutions.
(0.82 MB TIF)
Unrooted dendrogram of glycoside hydrolase family 9 modules detected in R. flavefaciens FD-1 compared with those from other organisms. “Rf” refers to R. flavefaciens, and the ORF number refers to TIGR's Annotation Engine designation. The scale bar indicates the percentage (0.1) of amino acid substitutions.
(0.77 MB TIF)
(0.04 MB XLS)
(0.04 MB XLS)
(0.09 MB DOC)
(0.02 MB XLS)
(0.11 MB DOC)
(0.14 MB DOC)
(0.10 MB DOC)
(0.03 MB XLS)
(0.05 MB XLS)
(0.02 MB XLS)
(0.02 MB XLS)
(0.02 MB XLS)
The authors wishes to acknowledge the staff of the W.M. Keck Center for Comparative and Functional Genomics including Ryan Kim, Lei Liu, Sharon Bachman, Jennifer Edwards, Laura Guest, John Moore, Peter Schweitzer, and Chris Wright. The authors also thank Peter Reilly and Chris Warner at Iowa State University for alerting us to the sequence errors in the published celB sequence.
Conceived and designed the experiments: MEBM DA BAW. Performed the experiments: MEBM DA. Analyzed the data: MEBM DA MTR JT BH PMC IB SJ RL HJF EAB. Contributed reagents/materials/analysis tools: MB AB TA AGH JT. Wrote the paper: MEBM DA BAW.
- 1. Wolin MJ, Miller LT (1983) Interactions of microbial populations in cellulose fermentation. Federation Proceedings 42: 109–113.MJ WolinLT Miller1983Interactions of microbial populations in cellulose fermentation.Federation Proceedings42109113
- 2. Hungate RE (1947) Studies on cellulose fermentation III. The culture and isolation of cellulose-decomposing bacteria from the rumen of cattle. J Bacteriol 53: 631–645.RE Hungate1947Studies on cellulose fermentation III. The culture and isolation of cellulose-decomposing bacteria from the rumen of cattle.J Bacteriol53631645
- 3. Sijpesteijn AK (1951) On Ruminococcus flavefaciens, a cellulose decomposing bacterium from the rumen of sheep and cattle. J Gen Microbiol 5: 869–879.AK Sijpesteijn1951On Ruminococcus flavefaciens, a cellulose decomposing bacterium from the rumen of sheep and cattle.J Gen Microbiol5869879
- 4. Bryant MP, Small N, Bouma C, Robinson IM (1958) Characteristics of ruminal anaerobic celluloytic cocci and Cillobacterium cellulosolvens n. sp. J Bacteriol 76: 529–537.MP BryantN. SmallC. BoumaIM Robinson1958Characteristics of ruminal anaerobic celluloytic cocci and Cillobacterium cellulosolvens n. sp.J Bacteriol76529537
- 5. Bryant MP (1986) Ruminococcus. In: Sneath PH, Mair HS, Sharpe ME, Holt JG, editors. Bergey's Manual of Systematic Bacteriology. Baltimore: Williams and Wilkins Co. pp. 1093–1097.MP Bryant1986Ruminococcus.PH SneathHS MairME SharpeJG HoltBergey's Manual of Systematic BacteriologyBaltimoreWilliams and Wilkins Co10931097
- 6. Dehority BA, Scott HW (1967) Extent of cellulose and hemicellulose digestion in various forages by pure cultures of rumen bacteria. J Dairy Science 50: 1136–1141.BA DehorityHW Scott1967Extent of cellulose and hemicellulose digestion in various forages by pure cultures of rumen bacteria.J Dairy Science5011361141
- 7. Helaszek CT, White BA (1991) Cellobiose uptake and metabolism by Ruminococcus flavefaciens. Appl Environ Microbiol 57: 64–68.CT HelaszekBA White1991Cellobiose uptake and metabolism by Ruminococcus flavefaciens.Appl Environ Microbiol576468
- 8. Robert C, Bernalier-Donadille A (2003) The cellulolytic microflora of the human colon: evidence of microcrystalline cellulose-degrading bacteria in methane-excreting subjects. FEMS Microbiol Ecol 46: 81–89.C. RobertA. Bernalier-Donadille2003The cellulolytic microflora of the human colon: evidence of microcrystalline cellulose-degrading bacteria in methane-excreting subjects.FEMS Microbiol Ecol468189
- 9. Pettipher GL, Latham MJ (1979) Characteristics of enzymes produced by Ruminococcus flavefaciens that degrade plant cell walls. J Gen Microbiol 110: 21–27.GL PettipherMJ Latham1979Characteristics of enzymes produced by Ruminococcus flavefaciens that degrade plant cell walls.J Gen Microbiol1102127
- 10. Gardner RM, Doerner KC, White BA (1987) Purification and characterization of an exo-beta-1,4-glucanase from Ruminococcus flavefaciens FD-1. J Bacteriol 169: 4581–4588.RM GardnerKC DoernerBA White1987Purification and characterization of an exo-beta-1,4-glucanase from Ruminococcus flavefaciens FD-1.J Bacteriol16945814588
- 11. Rasmussen MA, Hespell RB, White BA, Bothast RJ (1988) Inhibitory effects of methylcellulose on cellulose degradation by Ruminococcus flavefaciens. Appl Environ Microbiol 54: 890–897.MA RasmussenRB HespellBA WhiteRJ Bothast1988Inhibitory effects of methylcellulose on cellulose degradation by Ruminococcus flavefaciens.Appl Environ Microbiol54890897
- 12. Doerner KC, White BA (1990) Assessment of the endo-1,4-beta-glucanase components of Ruminococcus flavefaciens FD-1. Appl Environ Microbiol 56: 1844–1850.KC DoernerBA White1990Assessment of the endo-1,4-beta-glucanase components of Ruminococcus flavefaciens FD-1.Appl Environ Microbiol5618441850
- 13. Lamed R, Naimark J, Morgenstern E, Bayer EA (1987) Specialized cell surface structures in cellulolytic bacteria. J Bacteriol 169: 3792–3800.R. LamedJ. NaimarkE. MorgensternEA Bayer1987Specialized cell surface structures in cellulolytic bacteria.J Bacteriol16937923800
- 14. Wang WY, Thomson JA (1990) Nucleotide sequence of the celA gene encoding a cellodextrinase of Ruminococcus flavefaciens FD-1. Mol Gen Genet 222: 265–269.WY WangJA Thomson1990Nucleotide sequence of the celA gene encoding a cellodextrinase of Ruminococcus flavefaciens FD-1.Mol Gen Genet222265269
- 15. White BA, Clarke JH, Doerner KC, Gupta VK, Helaszek CT, et al. (1990) Improving cellulase activity in Ruminococcus through genetic manipulation. In: Akin DE, Ljungdahl LG, Wilson JR, Harris PJ, editors. Microbial and Plant Oppurtunitites to Improve Lignocellulose Utilization by Ruminants. New York City, NY: Elsevier Scientific Publishing. BA WhiteJH ClarkeKC DoernerVK GuptaCT Helaszek1990Improving cellulase activity in Ruminococcus through genetic manipulation.DE AkinLG LjungdahlJR WilsonPJ HarrisMicrobial and Plant Oppurtunitites to Improve Lignocellulose Utilization by RuminantsNew York City, NYElsevier Scientific Publishing
- 16. Wang W, Reid SJ, Thomson JA (1993) Transcriptional regulation of an endoglucanase and a cellodextrinase gene in Ruminococcus flavefaciens FD-1. J Gen Microbiol 139: 1219–1226.W. WangSJ ReidJA Thomson1993Transcriptional regulation of an endoglucanase and a cellodextrinase gene in Ruminococcus flavefaciens FD-1.J Gen Microbiol13912191226
- 17. Vercoe PE, Finks JL, White BA (1995) DNA sequence and transcriptional characterization of a beta-glucanase gene (celB) from Ruminococcus flavefaciens FD-1. Can J Microbiol 41: 869–876.PE VercoeJL FinksBA White1995DNA sequence and transcriptional characterization of a beta-glucanase gene (celB) from Ruminococcus flavefaciens FD-1.Can J Microbiol41869876
- 18. Vercoe PE, Spight DH, White BA (1995) Nucleotide sequence and transcriptional analysis of the celD beta-glucanase gene from Ruminococcus flavefaciens FD-1. Can J Microbiol 41: 27–34.PE VercoeDH SpightBA White1995Nucleotide sequence and transcriptional analysis of the celD beta-glucanase gene from Ruminococcus flavefaciens FD-1.Can J Microbiol412734
- 19. Aurilia V, Martin JC, McCrae SI, Scott KP, Rincon MT, et al. (2000) Three multidomain esterases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that carry divergent dockerin sequences. Microbiology 146 (Pt 6): 1391–1397.V. AuriliaJC MartinSI McCraeKP ScottMT Rincon2000Three multidomain esterases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that carry divergent dockerin sequences.Microbiology146 (Pt 6)13911397
- 20. Flint HJ, Martin J, McPherson CA, Daniel AS, Zhang JX (1993) A bifunctional enzyme, with separate xylanase and beta(1,3-1,4)-glucanase domains, encoded by the xynD gene of Ruminococcus flavefaciens. J Bacteriol 175: 2943–2951.HJ FlintJ. MartinCA McPhersonAS DanielJX Zhang1993A bifunctional enzyme, with separate xylanase and beta(1,3-1,4)-glucanase domains, encoded by the xynD gene of Ruminococcus flavefaciens.J Bacteriol17529432951
- 21. Zhang JX, Flint HJ (1992) A bifunctional xylanase encoded by the xynA gene of the rumen cellulolytic bacterium Ruminococcus flavefaciens 17 comprises two dissimilar domains linked by an asparagine/glutamine-rich sequence. Mol Microbiol 6: 1013–1023.JX ZhangHJ Flint1992A bifunctional xylanase encoded by the xynA gene of the rumen cellulolytic bacterium Ruminococcus flavefaciens 17 comprises two dissimilar domains linked by an asparagine/glutamine-rich sequence.Mol Microbiol610131023
- 22. Kirby J, Martin JC, Daniel AS, Flint HJ (1997) Dockerin-like sequences in cellulases and xylanases from the rumen cellulolytic bacterium Ruminococcus flavefaciens. FEMS Microbiol Lett 149: 213–219.J. KirbyJC MartinAS DanielHJ Flint1997Dockerin-like sequences in cellulases and xylanases from the rumen cellulolytic bacterium Ruminococcus flavefaciens.FEMS Microbiol Lett149213219
- 23. Rincon MT, McCrae SI, Kirby J, Scott KP, Flint HJ (2001) EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, binds to cellulose via a novel cellulose-binding module and to another R. flavefaciens protein via a dockerin domain. Appl Environ Microbiol 67: 4426–4431.MT RinconSI McCraeJ. KirbyKP ScottHJ Flint2001EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, binds to cellulose via a novel cellulose-binding module and to another R. flavefaciens protein via a dockerin domain.Appl Environ Microbiol6744264431
- 24. Ding SY, Rincon MT, Lamed R, Martin JC, McCrae SI, et al. (2001) Cellulosomal scaffoldin-like proteins from Ruminococcus flavefaciens. J Bacteriol 183: 1945–1953.SY DingMT RinconR. LamedJC MartinSI McCrae2001Cellulosomal scaffoldin-like proteins from Ruminococcus flavefaciens.J Bacteriol18319451953
- 25. Rincon MT, Cepeljnik T, Martin JC, Lamed R, Barak Y, et al. (2005) Unconventional mode of attachment of the Ruminococcus flavefaciens cellulosome to the cell surface. J Bacteriol 187: 7569–7578.MT RinconT. CepeljnikJC MartinR. LamedY. Barak2005Unconventional mode of attachment of the Ruminococcus flavefaciens cellulosome to the cell surface.J Bacteriol18775697578
- 26. Rincon MT, Ding SY, McCrae SI, Martin JC, Aurilia V, et al. (2003) Novel organization and divergent dockerin specificities in the cellulosome system of Ruminococcus flavefaciens. J Bacteriol 185: 703–713.MT RinconSY DingSI McCraeJC MartinV. Aurilia2003Novel organization and divergent dockerin specificities in the cellulosome system of Ruminococcus flavefaciens.J Bacteriol185703713
- 27. Rincon MT, Martin JC, Aurilia V, McCrae SI, Rucklidge GJ, et al. (2004) ScaC, an adaptor protein carrying a novel cohesin that expands the dockerin-binding repertoire of the Ruminococcus flavefaciens 17 cellulosome. J Bacteriol 186: 2576–2585.MT RinconJC MartinV. AuriliaSI McCraeGJ Rucklidge2004ScaC, an adaptor protein carrying a novel cohesin that expands the dockerin-binding repertoire of the Ruminococcus flavefaciens 17 cellulosome.J Bacteriol18625762585
- 28. Jindou S, Borovok I, Rincon MT, Flint HJ, Antonopoulos DA, et al. (2006) Conservation and divergence in cellulosome architecture between two strains of Ruminococcus flavefaciens. J Bacteriol 188: 7971–7976.S. JindouI. BorovokMT RinconHJ FlintDA Antonopoulos2006Conservation and divergence in cellulosome architecture between two strains of Ruminococcus flavefaciens.J Bacteriol18879717976
- 29. Antonopoulos DA, Russell WM, White BA (2003) Phylogenetic reconstruction of Gram-positive organisms based on comparative sequence analysis of molecular chaperones from the ruminal microorganism Ruminococcus flavefaciens FD-1. FEMS Microbiol Lett 227: 1–7.DA AntonopoulosWM RussellBA White2003Phylogenetic reconstruction of Gram-positive organisms based on comparative sequence analysis of molecular chaperones from the ruminal microorganism Ruminococcus flavefaciens FD-1.FEMS Microbiol Lett22717
- 30. Antonopoulos DA, Nelson KE, Morrison M, White BA (2004) Strain-specific genomic regions of Ruminococcus flavefaciens FD-1 as revealed by combinatorial random-phase genome sequencing and suppressive subtractive hybridization. Environ Microbiol 6: 335–346.DA AntonopoulosKE NelsonM. MorrisonBA White2004Strain-specific genomic regions of Ruminococcus flavefaciens FD-1 as revealed by combinatorial random-phase genome sequencing and suppressive subtractive hybridization.Environ Microbiol6335346
- 31. Ewing B, Hillier L, Wendl MC, Green P (1998) Base-calling of automated sequencer traces using Phred. I. Accuracy assessment. Genome Research 8: 175–185.B. EwingL. HillierMC WendlP. Green1998Base-calling of automated sequencer traces using Phred. I. Accuracy assessment.Genome Research8175185
- 32. Ewing B, Green P (1998) Base-calling of automated sequencer traces using Phred. II. Error probabilities. Genome Research 8: 186–194.B. EwingP. Green1998Base-calling of automated sequencer traces using Phred. II. Error probabilities.Genome Research8186194
- 33. Gordon D, Abajian C, Green P (1998) Consed: a graphical tool for sequencing finishing. Genome Research 8: 195–202.D. GordonC. AbajianP. Green1998Consed: a graphical tool for sequencing finishing.Genome Research8195202
- 34. Selkov E, Overbeek R, Kogan Y, Chu L, Vonstein V, et al. (2000) Functional analysis of gapped microbial genomes: amino acid metabolism of Thiobacillus ferrooxidans. Proc Natl Acad Sci U S A 97: 3509–3514.E. SelkovR. OverbeekY. KoganL. ChuV. Vonstein2000Functional analysis of gapped microbial genomes: amino acid metabolism of Thiobacillus ferrooxidans.Proc Natl Acad Sci U S A9735093514
- 35. Bryant MP, Robinson IM (1961) Studies on the nitrogen requirements of some ruminal cellulolytic bacteria. Appl Microbiol 9: 96–103.MP BryantIM Robinson1961Studies on the nitrogen requirements of some ruminal cellulolytic bacteria.Appl Microbiol996103
- 36. Bryant MP, Robinson IM (1963) Apparent incorporation of ammonia and amino acid carbon during growth of selected species of ruminal bacteria. J Dairy Sci 46: 150–154.MP BryantIM Robinson1963Apparent incorporation of ammonia and amino acid carbon during growth of selected species of ruminal bacteria.J Dairy Sci46150154
- 37. Krieger CJ, Zhang P, Mueller LA, Wang A, Paley S, et al. (2004) MetaCyc: a multiorganism database of metabolic pathways and enzymes. Nucleic Acids Res 32 Database issue D438–442.CJ KriegerP. ZhangLA MuellerA. WangS. Paley2004MetaCyc: a multiorganism database of metabolic pathways and enzymes.Nucleic Acids Res 32 Database issueD438442
- 38. Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, et al. (2009) The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic Acids Res 37: D233–238.BL CantarelPM CoutinhoC. RancurelT. BernardV. Lombard2009The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics.Nucleic Acids Res37D233238
- 39. Devillard E, Goodheart DB, Karnati SK, Bayer EA, Lamed R, et al. (2004) Ruminococcus albus 8 mutants defective in cellulose degradation are deficient in two processive endocellulases, Cel48A and Cel9B, both of which possess a novel modular architecture. J Bacteriol 186: 136–145.E. DevillardDB GoodheartSK KarnatiEA BayerR. Lamed2004Ruminococcus albus 8 mutants defective in cellulose degradation are deficient in two processive endocellulases, Cel48A and Cel9B, both of which possess a novel modular architecture.J Bacteriol186136145
- 40. Aurilia E, Martin JC, Scott KP, Mercer DK, Johnston MEA, et al. (2000) Organisation and Variable Incidence of Genes Concerned with the Utilization of Xylans in the Rumen Cellulolytic Bacterium Ruminococcus flavefaciens. Anaerobe 6: 333–340.E. AuriliaJC MartinKP ScottDK MercerMEA Johnston2000Organisation and Variable Incidence of Genes Concerned with the Utilization of Xylans in the Rumen Cellulolytic Bacterium Ruminococcus flavefaciens.Anaerobe6333340
- 41. Flint HJ, McPherson CA, Bisset J (1989) Molecular cloning of genes from Ruminococcus flavefaciens encoding xylanase and beta(1-3,1-4)glucanase activities. Appl Environ Microbiol 55: 1230–1233.HJ FlintCA McPhersonJ. Bisset1989Molecular cloning of genes from Ruminococcus flavefaciens encoding xylanase and beta(1-3,1-4)glucanase activities.Appl Environ Microbiol5512301233
- 42. Gilkes NR, Henrissat B, Kilburn DG, Miller RC Jr, Warren RA (1991) Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. Microbiol Rev 55: 303–315.NR GilkesB. HenrissatDG KilburnRC Miller JrRA Warren1991Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.Microbiol Rev55303315
- 43. Bayer EA, Chanzy H, Lamed R, Shoham Y (1998) Cellulose, cellulases and cellulosomes. Curr Opin Struct Biol 8: 548–557.EA BayerH. ChanzyR. LamedY. Shoham1998Cellulose, cellulases and cellulosomes.Curr Opin Struct Biol8548557
- 44. Reverbel-Leroy C, Pages S, Belaich A, Belaich JP, Tardif C (1997) The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome: purification and characterization of the recombinant form. J Bacteriol 179: 46–52.C. Reverbel-LeroyS. PagesA. BelaichJP BelaichC. Tardif1997The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome: purification and characterization of the recombinant form.J Bacteriol1794652
- 45. Sakon J, Irwin D, Wilson DB, Karplus PA (1997) Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca. Nature Struct Biol 4: 810–818.J. SakonD. IrwinDB WilsonPA Karplus1997Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca.Nature Struct Biol4810818
- 46. Rincon MT, Cepeljnik T, Martin JC, Barak Y, Lamed R, et al. (2007) A novel cell surface-anchored cellulose-binding protein encoded by the sca gene cluster of Ruminococcus flavefaciens. J Bacteriol 189: 4774–4783.MT RinconT. CepeljnikJC MartinY. BarakR. Lamed2007A novel cell surface-anchored cellulose-binding protein encoded by the sca gene cluster of Ruminococcus flavefaciens.J Bacteriol18947744783
- 47. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882.JD ThompsonTJ GibsonF. PlewniakF. JeanmouginDG Higgins1997The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.Nucleic Acids Res2548764882
- 48. Rincon MT, Martin JC, Aurilia V, McCrae SI, Rucklidge GJ, et al. (2004) ScaC, an adaptor protein carrying a novel cohesin that expands the dockerin-binding repertoire of the Ruminococcus flavefaciens 17 cellulosome. J Bacteriol 186: 2576–2585.MT RinconJC MartinV. AuriliaSI McCraeGJ Rucklidge2004ScaC, an adaptor protein carrying a novel cohesin that expands the dockerin-binding repertoire of the Ruminococcus flavefaciens 17 cellulosome.J Bacteriol18625762585
- 49. Aurilia V, Martin JC, McCrae SI, Scott KP, Rincon MT, et al. (2000) Three multidomain esterases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that carry divergent dockerin sequences. Microbiology 146: 1391–1397.V. AuriliaJC MartinSI McCraeKP ScottMT Rincon2000Three multidomain esterases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that carry divergent dockerin sequences.Microbiology14613911397
- 50. Ohara H, Karita S, Kimura T, Sakka K, Ohmiya K (2000) Characterization of the cellulolytic complex (cellulosome) from Ruminococcus albus. Biosci Biotechnol Biochem 64: 254–260.H. OharaS. KaritaT. KimuraK. SakkaK. Ohmiya2000Characterization of the cellulolytic complex (cellulosome) from Ruminococcus albus.Biosci Biotechnol Biochem64254260
- 51. Ohara H, Noguchi J, Karita S, Kimura T, Sakka K, et al. (2000) Sequence of egV and properties of EgV, a Ruminococcus albus endoglucanase containing a dockerin domain. Biosci Biotechnol Biochem 64: 80–88.H. OharaJ. NoguchiS. KaritaT. KimuraK. Sakka2000Sequence of egV and properties of EgV, a Ruminococcus albus endoglucanase containing a dockerin domain.Biosci Biotechnol Biochem648088
- 52. Kobe B, Kajava AV (2001) The leucine-rich repeat as a protein recognition motif. Curr Opin Struct Biol 11: 725–732.B. KobeAV Kajava2001The leucine-rich repeat as a protein recognition motif.Curr Opin Struct Biol11725732
- 53. Brulc JM, Antonopoulos DA, Berg Miller ME, Wilson MK, Yannarell AC, et al. (2009) Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases. Proc Natl Acad Sci U S A 106: 1948–1953.JM BrulcDA AntonopoulosME Berg MillerMK WilsonAC Yannarell2009Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases.Proc Natl Acad Sci U S A10619481953
- 54. Bron PA, Molenaar D, de Vos WM, Kleerebezem M (2006) DNA micro-array-based identification of bile-responsive genes in Lactobacillus plantarum. J Appl Microbiol 100: 728–738.PA BronD. MolenaarWM de VosM. Kleerebezem2006DNA micro-array-based identification of bile-responsive genes in Lactobacillus plantarum.J Appl Microbiol100728738
- 55. Fierobe HP, Bayer EA, Tardif C, Czjzek M, Mechaly A, et al. (2002) Degradation of cellulose substrates by cellulosome chimeras. Substrate targeting versus proximity of enzyme components. J Biol Chem 277: 49621–49630.HP FierobeEA BayerC. TardifM. CzjzekA. Mechaly2002Degradation of cellulose substrates by cellulosome chimeras. Substrate targeting versus proximity of enzyme components.J Biol Chem2774962149630
- 56. Fierobe HP, Mingardon F, Mechaly A, Belaich A, Rincon MT, et al. (2005) Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined trifunctional scaffoldin. J Biol Chem 280: 16325–16334.HP FierobeF. MingardonA. MechalyA. BelaichMT Rincon2005Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined trifunctional scaffoldin.J Biol Chem2801632516334
- 57. Zverlov VV, Kellermann J, Schwarz WH (2005) Functional subgenomics of Clostridium thermocellum cellulosomal genes: identification of the major catalytic components in the extracellular complex and detection of three new enzymes. Proteomics 5: 3646–3653.VV ZverlovJ. KellermannWH Schwarz2005Functional subgenomics of Clostridium thermocellum cellulosomal genes: identification of the major catalytic components in the extracellular complex and detection of three new enzymes.Proteomics536463653
- 58. Doerner KC, Howard GT, Mackie RI, White BA (1992) b-Glucanase expression by Ruminococcus flavefaciens FD-1. FEMS Microbiology Letters 93: 147–157.KC DoernerGT HowardRI MackieBA White1992b-Glucanase expression by Ruminococcus flavefaciens FD-1.FEMS Microbiology Letters93147157
- 59. Wang W, Reid SJ, Thomson JA (1993) Transcriptional regulation of an endoglucanase and a cellodextrinase gene in Ruminococcus flavefaciens FD-1. J Gen Microbiol 139 Pt 6: 1219–1226.W. WangSJ ReidJA Thomson1993Transcriptional regulation of an endoglucanase and a cellodextrinase gene in Ruminococcus flavefaciens FD-1.J Gen Microbiol139 Pt 612191226
- 60. Dror TW, Morag E, Rolider A, Bayer EA, Lamed R, et al. (2003) Regulation of the cellulosomal CelS (cel48A) gene of Clostridium thermocellum is growth rate dependent. J Bacteriol 185: 3042–3048.TW DrorE. MoragA. RoliderEA BayerR. Lamed2003Regulation of the cellulosomal CelS (cel48A) gene of Clostridium thermocellum is growth rate dependent.J Bacteriol18530423048
- 61. Gold ND, Martin VJ (2007) Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis. J Bacteriol 189: 6787–6795.ND GoldVJ Martin2007Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis.J Bacteriol18967876795
- 62. Stevenson DM, Weimer PJ (2005) Expression of 17 genes in Clostridium thermocellum ATCC 27405 during fermentation of cellulose or cellobiose in continuous culture. Appl Environ Microbiol 71: 4672–4678.DM StevensonPJ Weimer2005Expression of 17 genes in Clostridium thermocellum ATCC 27405 during fermentation of cellulose or cellobiose in continuous culture.Appl Environ Microbiol7146724678
- 63. Kwinn LA, Khosravi A, Aziz RK, Timmer AM, Doran KS, et al. (2007) Genetic characterization and virulence role of the RALP3/LSA locus upstream of the streptolysin s operon in invasive M1T1 Group A Streptococcus. Journal of Bacteriology 189: 1322–1329.LA KwinnA. KhosraviRK AzizAM TimmerKS Doran2007Genetic characterization and virulence role of the RALP3/LSA locus upstream of the streptolysin s operon in invasive M1T1 Group A Streptococcus.Journal of Bacteriology18913221329
- 64. Mongodin E, Finan J, Climo MW, Rosato A, Gill S, et al. (2003) Microarray transcription analysis of clinical Staphylococcus aureus isolates resistant to vancomycin. J Bacteriol 185: 4638–4643.E. MongodinJ. FinanMW ClimoA. RosatoS. Gill2003Microarray transcription analysis of clinical Staphylococcus aureus isolates resistant to vancomycin.J Bacteriol18546384643
- 65. Réglier-Poupet H, Frehel C, Dubail I, Beretti JL, Berche P, et al. (2003) Maturation of lipoproteins by type II signal peptidase is required for phagosomal escape of Listeria monocytogenes. J Biol Chem 278: 49469–49477.H. Réglier-PoupetC. FrehelI. DubailJL BerettiP. Berche2003Maturation of lipoproteins by type II signal peptidase is required for phagosomal escape of Listeria monocytogenes.J Biol Chem2784946949477
- 66. Salim KY, de Azavedo JC, Bast DJ, Cvitkovitch DG (2007) Role for sagA and siaA in quorum sensing and iron regulation in Streptococcus pyogenes. Infection and Immunity 75: 5011–5017.KY SalimJC de AzavedoDJ BastDG Cvitkovitch2007Role for sagA and siaA in quorum sensing and iron regulation in Streptococcus pyogenes.Infection and Immunity7550115017
- 67. Wong ML, Medrano JF (2005) Real-time PCR for mRNA quantitation. Biotechniques 39: 75–85.ML WongJF Medrano2005Real-time PCR for mRNA quantitation.Biotechniques397585
- 68. Morey JS, Ryan JC, Van Dolah FM (2006) Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR. Biol Proced Online 8: 175–193.JS MoreyJC RyanFM Van Dolah2006Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR.Biol Proced Online8175193
- 69. Bayer EA, Shoham Y, Lamed R (2000) Cellulose-decomposing prokaryotes and their enzyme systems. In: Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E, editors. The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community. New York City, New York: Springer-Verlag. EA BayerY. ShohamR. Lamed2000Cellulose-decomposing prokaryotes and their enzyme systems.M. DworkinS. FalkowE. RosenbergK-H SchleiferE. StackebrandtThe Prokaryotes: An Evolving Electronic Resource for the Microbiological CommunityNew York City, New YorkSpringer-Verlag3 ed. 3 ed.
- 70. Flint HJ, Zhang JX, Martin J (1994) Multiplicity and Expression of Xylanases in the Rumen Cellulolytic Bacterium Ruminococcus flavefaciens. Current Microbiology 29: 139–143.HJ FlintJX ZhangJ. Martin1994Multiplicity and Expression of Xylanases in the Rumen Cellulolytic Bacterium Ruminococcus flavefaciens.Current Microbiology29139143
- 71. Antonopoulos DA, Aminov RI, Duncan PA, White BA, Mackie RI (2003) Characterization of the gene encoding glutamate dehydrogenase (gdhA) from the ruminal bacterium Ruminococcus flavefaciens FD-1. Arch Microbiol 179: 184–190.DA AntonopoulosRI AminovPA DuncanBA WhiteRI Mackie2003Characterization of the gene encoding glutamate dehydrogenase (gdhA) from the ruminal bacterium Ruminococcus flavefaciens FD-1.Arch Microbiol179184190
- 72. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402.SF AltschulTL MaddenAA SchäfferJ. ZhangZ. Zhang1997Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.Nucleic Acids Res2533893402
- 73. Pearson WR (1994) Using the FASTA program to search protein and DNA sequence databases. Methods Mol Biol 24: 307–331.WR Pearson1994Using the FASTA program to search protein and DNA sequence databases.Methods Mol Biol24307331
- 74. Salzberg SL, Delcher AL, Kasif S, White O (1998) Microbial gene identification using interpolated Markov models. Nucleic Acids Res 26: 544–548.SL SalzbergAL DelcherS. KasifO. White1998Microbial gene identification using interpolated Markov models.Nucleic Acids Res26544548
- 75. Delcher AL, Harmon D, Kasif S, White O, Salzberg SL (1999) Improved microbial gene identification with GLIMMER. Nucleic Acids Res 27: 4636–4641.AL DelcherD. HarmonS. KasifO. WhiteSL Salzberg1999Improved microbial gene identification with GLIMMER.Nucleic Acids Res2746364641
- 76. Eddy SR (1998) Profile hidden Markov models. Bioinformatics 14: 755–763.SR Eddy1998Profile hidden Markov models.Bioinformatics14755763
- 77. Bryant MP, Burkey LA (1953) Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J Dairy Sci 36: 205–217.MP BryantLA Burkey1953Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen.J Dairy Sci36205217
- 78. Allison MJ, Bryant MP, Doetsch RN (1958) Volatile fatty acid growth factor for cellulolytic cocci of bovine rumen. Science 128: 474–475.MJ AllisonMP BryantRN Doetsch1958Volatile fatty acid growth factor for cellulolytic cocci of bovine rumen.Science128474475
- 79. Odenyo AA, Mackie RI, Stahl DA, White BA (1994) The use of 16S rRNA-targeted oligonucleotide probes to study competition between ruminal fibrolytic bacteria: development of probes for Ruminococcus species and evidence for bacteriocin production. Appl Environ Microbiol 60: 3688–3696.AA OdenyoRI MackieDA StahlBA White1994The use of 16S rRNA-targeted oligonucleotide probes to study competition between ruminal fibrolytic bacteria: development of probes for Ruminococcus species and evidence for bacteriocin production.Appl Environ Microbiol6036883696
- 80. Goerke C, Fluckiger U, Steinhuber A, Zimmerli W, Wolz C (2001) Impact of the regulatory loci agr, sarA and sae of Staphylococcus aureus on the induction of alpha-toxin during device-related infection resolved by direct quantitative transcript analysis. Mol Microbiol 40: 1439–1447.C. GoerkeU. FluckigerA. SteinhuberW. ZimmerliC. Wolz2001Impact of the regulatory loci agr, sarA and sae of Staphylococcus aureus on the induction of alpha-toxin during device-related infection resolved by direct quantitative transcript analysis.Mol Microbiol4014391447