Recent studies have identified several single nucleotide polymorphisms (SNPs) in the population that are associated with variations in the risks of many different diseases including cancers such as breast, prostate and colorectal. For ovarian cancer, the known highly penetrant susceptibility genes (BRCA1 and BRCA2) are probably responsible for only 40% of the excess familial ovarian cancer risks, suggesting that other susceptibility genes of lower penetrance exist.
We have taken a candidate approach to identifying moderate risk susceptibility alleles for ovarian cancer. To date, we have genotyped 340 SNPs from 94 candidate genes or regions, in up to 1,491 invasive epithelial ovarian cancer cases and 3,145 unaffected controls from three different population based studies from the UK, Denmark and USA.
After adjusting for population stratification by genomic control, 18 SNPs (5.3%) were significant at the 5% level, and 5 SNPs (1.5%) were significant at the 1% level. The most significant association was for the SNP rs2107425, located on chromosome 11p15.5, which has previously been identified as a susceptibility allele for breast cancer from a genome wide association study (P-trend = 0.0012). When SNPs/genes were stratified into 7 different pathways or groups of validation SNPs, the breast cancer associated SNPs were the only group of SNPs that were significantly associated with ovarian cancer risk (P-heterogeneity = 0.0003; P-trend = 0.0028; adjusted (for population stratification) P-trend = 0.006). We did not find statistically significant associations when the combined data for all SNPs were analysed using an admixture maximum likelihood (AML) experiment-wise test for association (P-heterogeneity = 0.051; P-trend = 0.068).
Citation: Quaye L, Tyrer J, Ramus SJ, Song H, Wozniak E, DiCioccio RA, et al. (2009) Association between Common Germline Genetic Variation in 94 Candidate Genes or Regions and Risks of Invasive Epithelial Ovarian Cancer. PLoS ONE 4(6): e5983. https://doi.org/10.1371/journal.pone.0005983
Editor: Amanda Ewart Toland, Ohio State University Medical Center, United States of America
Received: February 13, 2009; Accepted: May 8, 2009; Published: June 19, 2009
Copyright: © 2009 Quaye et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: LQ is funded by the MRC. SJR is funded by the Mermaid component of the Eve Appeal, HS is funded by a grant from WellBeing of Women, DFE is a Principal Research Fellow of Cancer Research UK, PDPP is a Senior Clinical Research Fellow. The research was funded by a Cancer Research UK project grant (no. C8804/A7058). Additional support was provided by the Roswell Park Alliance and the National Cancer Institute (CA71766 and Core Grant CA16056). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
One of the strongest risk factors for invasive epithelial ovarian cancer is a family history of the disease; a woman with a single first-degree relative diagnosed with ovarian cancer has a 2–3 fold increased risk . Familial aggregation of cancer could be due to either a sharing of environmental or lifestyle risk factors within families, or through the inheritance of genetic risk factors. The higher rates of cancer in monozoygotic twins, compared with dizygotic twins or other siblings of individuals with cancer, suggests that genetic factors play a greater role .
Germline mutations in the high penetrance genes BRCA1 and BRCA2 are responsible for the vast majority of families containing multiple cases of ovarian cancer (>3 cases) and two or more cases of breast and ovarian cancer –. Other highly penetrant genes may exist, but these are likely to be rare and account for only a small fraction of the excess familial risk. The risk of ovarian cancer in first-degree relatives of ovarian cancer patients compared to the population risk is estimated to be about 2.4 . Approximately 60% of this familial relative risk is not accounted for by the known high risk loci . This remaining risk is likely to be caused by a combination of common low penetrance genes (the common variant: common disease hypothesis) and/or rare variants of moderate penetrance .
Genetic association studies, which compare the frequencies of common genetic variants between cases and unaffected controls, have become the preferred approach to look for low penetrance cancer susceptibility genes. Candidate gene studies have focused on common variants in genes that may play a role in cancer (e.g. cell cycle control; DNA damage repair and response; proliferation and apoptosis) with some success –. However, they have often been limited by small sample sizes, which reduce the power to detect associations at very stringent levels of significance. Consortium based studies have been used to increase the samples size and attempt to validate preliminary results –. More recently, genome-wide association studies, an approach that compares the frequency of hundreds of thousands of SNPs distributed evenly throughout the genome, have been successful in identifying low penetrance susceptibility variants for breast, colorectal and prostate cancers –. There are, as yet, no published genome-wide association studies for ovarian cancer. Therefore, we have continued to take a candidate gene approach to identify ovarian cancer susceptibility markers. We have mainly used an empirical approach, in which a minimal set of ”tagging” single nucleotide polymorphisms (SNPs) that efficiently capture all the common genetic variation in a gene, to find genetic susceptibility markers for ovarian cancer –. The candidate genes in this study were involved in several cancer related pathways; but we have also analysed candidate SNPs from the Ovarian Cancer Association Consortium (OCAC) validation studies and from two recently completed breast cancer genome wide association studies , .
The analysis of genetic association studies inevitably involves a large number of statistical tests, and there has been much debate about how to correct for multiple hypothesis testing. This has usually been considered a hypothesis-testing problem in which the aim is to control the overall “experiment-wise” type I error. The null hypothesis is that there is no association between any of the SNPs being tested with disease, and the aim is to test whether this global null hypothesis of no association can be rejected. A variety of methods have been proposed to test the global null hypothesis –. One of these, the admixture maximum likelihood (AML) test, simultaneously estimates the proportion of associated SNPs and their typical effect size. The power of the AML test has been found to be similar to or better than all other tests (rank truncated product, unrestricted maximum likelihood, restricted space maximum likelihood, most significant SNP, Global χ2, Best subset χ2) across a wide range of scenarios for the alternative hypothesis . The simple Bonferroni correction performed best only when the number of associated SNPs was small (typically ≤3 or <5% of SNPs tested, whichever is smaller).
The AML method has been used to evaluate the overall evidence of association between 710 common variants in 117 candidate genes and breast cancer risk . The results of this study showed that a proportion of SNPs in these candidate genes were associated with breast cancer risk, but that the effects of individual SNPs were likely to be small. In the current study, we use the AML method to evaluate data from 12 previous studies – for global evidence of associations between the risk of invasive epithelial ovarian cancer and 340 SNPs from 84 genes and 10 different chromosomal regions – SNPs in these regions are not within known genes or open reading frames. Three population-based studies comprising approximately 1,500 cases of invasive epithelial ovarian cancer and 3,100 unaffected controls were analysed.
Materials and Methods
Ethics committee approval was obtained for the collection and genetic analysis of all samples, and an informed written consent was obtained from all participants. Ethics approvals were granted by; the Danish Ethical Committees of Copenhagen and Frederiksberg (MALOVA), Anglia and Oxford Multi Centre Research Ethics Committee (SEARCH) and the Institutional Review Boards of Stanford University School of Medicine and Roswell Park Cancer Institute (GEOCS).
Three population-based ovarian cancer case-control series were used in this research : MALOVA (446 cases, 1,221 controls) from Denmark, SEARCH (719 cases, 855 controls) from the UK, and GEOCS (325 cases, 429 controls) from the USA. The case collection for MALOVA ran from 1994 to 1999 in Danish counties with a gynaecological hospital department. Cases were women diagnosed with invasive epithelial ovarian cancer aged 30–80 years. Controls were age-matched (within 3 years) and were randomly drawn from Danish born women in the study area by means of the computerized Danish Central Population Register. SEARCH began recruitment in 1998 and covers the regions served by the East Anglian and West Midlands cancer registries in the UK. Eligible women were those diagnosed since 1991 with invasive epithelial ovarian cancer under the age of 70 years. Controls, aged 45–74 years, from the same geographical region as the cases, were from the Norfolk constituent of the European Prospective Investigation of Cancer (EPIC) cohort. The Genetic Epidemiology Ovarian Cancer Study (GEOCS, formerly known as FROC) recruited participants from 6 counties in the San Francisco Bay area USA from 1997 to 2002. Patients, aged between 23–64 years, with invasive epithelial ovarian cancer were identified via rapid case ascertainment through the Greater Bay Area Cancer Registry operated by the Northern California Cancer Centre as part of the SEER Program. Control women were identified through random-digit dial and were frequency-matched to cases on race/ethnicity and five-year age group. Cases in MALOVA were collected prospectively, GEOCS retrospectively and SEARCH both prospectively and retrospectively. For all three studies, DNA samples were extracted from blood by Whatman International. Further details of these studies have been published previously –.
Gene and tag SNP selection
Candidate gene selection was primarily based on biological pathways that are predicted to be involved in ovarian carcinogenesis. The major pathways evaluated were DNA double strand break repair, cell cycle control and DNA mismatch repair (MMR). We also analysed several known or candidate tumour suppressor genes and oncogenes for ovarian cancer and a series of genes that we identified from the analysis of an in vitro functional model of ovarian cancer . Finally, we analysed SNPs that have previously shown weak evidence of association with ovarian cancer risks and SNPs that are known to be associated with breast cancer risk. The pathways, genes and SNPs analysed are listed in Table S1. Further details on some of these genes and SNPs, and their association with ovarian cancer have been published –.
For most genes, a tagging SNP approach was used to select known common variants. Haploview and Tagger were used for the selection of common variants from the reference CEPH genotypes. The approach involved the tagging of common SNPs with a tagging SNP (tSNP) with a minimum r2 of 0.8. If a SNP was poorly correlated with other SNPs, then 2- or 3-marker haplotypes were used, (an approach called “aggressive tagging”), if the haplotypes tagged the SNP(s) with a minimum r2 of 0.8. SNP tagging reduces the number of SNPs that require genotyping in association studies. The MMR gene study was completed before tagging SNP approaches were widely used due to the lack of available information from the International HapMap Project; and so we analysed SNPs of varying frequencies selected from public databases such as the dbSNP database (http://www.ncbi.nlm.nih.gov/SNP) and from the NIEHS Environmental Genome Project (EGP) (http://egp.gs.washington.edu/) .
The genotyping methods used in these studies have been described previously (9–14). All assays were carried out in 384-well plates and included 12 duplicate samples per plate (3%). Genotypes were excluded if duplicate concordance rates for a study were <98%. Plates also included non-template negative test controls. Finally genotypes were excluded from the analysis if call rates were <90% per plate. The average call rates for these SNP were 94% in cases and 96% in controls. A list of the SNPs genotyped in this study is provided in Table S1.
Associations between invasive epithelial ovarian cancer and each SNP were assessed using two tests; the one-degree of freedom Cochran–Armitage trend test and the general two-degrees of freedom χ2 test (heterogeneity test). Both tests were stratified by study to account for any differences within the sample sets. The overall evidence for an excess of associations between common variants and ovarian cancer risk was evaluated with the AML method, which is described in detail in Tyrer et al . Briefly, the AML method formulates the alternative hypothesis in terms of the probability (α) that a given SNP is associated with disease and a measured effect size. When a SNP is associated with disease, the calculated χ2 statistic will be distributed, asymptotically, as a non-central χ2 distribution with the usual degrees of freedom and a non-centrality parameter η. The non-centrality parameter is a measure of the size of effect of the SNP, and is closely related to the contribution of the SNP to the genetic variance of the trait. The non-centrality parameter was assumed to be the same for all SNPs to make the model more parsimonious. This was an approximation but improves power if the non-centrality parameter is roughly the same for associated SNPs as fewer parameters have to be optimized. If η is assumed to be the same for each associated SNP, then both α and η can be estimated by maximum likelihood, and a test of the null hypothesis can then be obtained as a likelihood ratio test. In instances such as this, where some SNPs were correlated, pseudo-maximum likelihood estimates can still be produced by the same procedure, as if the SNPs were independent. Therefore the pseudo-maximum-likelihood method was applied to account for LD between SNPs. Simulation can subsequently be used to establish the statistical significance of the test. We applied the AML method using both the trend and heterogeneity tests. All analyses were adjusted for cryptic population stratification using the method described by Devlin et al , . Genotyping data from 280 randomly selected, unlinked SNPs were used to adjust for population stratification using the genomic control method . These genotyping data were from cases and controls derived from a breast cancer genome-wide association study . The inflation test statistic we used was based on the inflation seen in genomic control samples for the breast cancer study and was chosen to be slightly higher than the estimated inflation as a “conservative” correction. We therefore adjusted the p-trend by 10% (1.1) and p-heterogeneity by 5% (1.05) as a means of conservatively allowing for any cryptic population stratification. Statistical significance is at the 5% level unless stated otherwise.
We have genotyped 340 SNPs in up to 1,491 invasive epithelial ovarian cancer cases and 3,145 unaffected controls from three different population based studies from the UK, Denmark and USA. SNPs were either tagging SNPs located in 84 candidate genes from pathways implicated in ovarian cancer development, or candidate SNPs located in 10 different regions on chromosomes 2, 3, 5, 8, 11, 12 and 17 that had been chosen for validation by the Ovarian Cancer Association Consortium (OCAC) or had been identified in a breast cancer genome-wide association study –. Genotype frequencies for these SNPs in cases and controls are given in Table S1.
Using the trend test for association, 22 SNPs (6.5%) were significant at the 5% level, and 5 SNPs (1.5%) were significant at the 1% level. After adjusting for population stratification by genomic control, 18 SNPs (5.3%) were significant at the 5% level, and 5 SNPs (1.5%) were significant at the 1% level (Table 1). The test results for every SNP are given in Table S2. Figure 1 illustrates the results of the univariate trend test shown as a quantile-quantile (Q-Q) plot, in which the ordered test statistics are plotted against the expected statistics given the rank. The Q–Q plot follows the line of equivalence for the first 240 SNPs and then starts to deviate, as would be expected if a modest proportion of SNPs were associated with disease.
The ordered test statistics are plotted against the expected statistics given the rank.
Of the 22 SNPs that were significant at the 5% level, three were SNPs that had been selected because of their association with breast cancer in genome wide association studies (of 16 in that group), eight were from the cell-cycle control pathway (of 101), two were from the DNA mismatch repair pathway (of 43), one was from the DNA double strand break repair pathway (of 28), two were from the MMCT-18 (functional candidate gene) group (of 63) and five were from the OCAC group of SNPs (of 55). However, no single SNP reached a level of significance to provide definitive evidence of association - the most significant association was for a breast cancer associated SNP, rs2107425, located on chromosome 11p15.5 (unadjusted P-trend = 0.0012). The SNP was still significantly associated with ovarian cancer risk after adjustment for population stratification (P-trend = 0.0019).
Table 2 shows the results of the AML experiment-wise tests summarised for the complete set of SNPs categorised according to functional group, biological pathway or genotyping group. The test for overall association was significant for the breast cancer associated group of SNPs identified by genome wide association studies (P-het. = 0.0003, P-trend = 0.0028; adjusted P-trend = 0.0059). No other group of SNPs was significant. When all the data were combined, the AML experiment-wise test for association was not significant for both the heterogeneity test (P = 0.051) and the trend test (P = 0.068). This suggests that, although not statistically significant, there is a trend towards a proportion of the SNPs evaluated being associated with disease and that the effect sizes were too small to detect for individual SNPs.
There are many studies in the published literature describing a candidate SNP/gene approach to search for common, germline genetic variants associated with epithelial ovarian cancer risk. These studies provide some evidence of association with disease risk for some SNPs –, , . In the current study, we have used data from 12 of these studies from three population based ovarian cancer case-control series. In total, 340 SNPs in 94 genes or regions were analysed in approximately 1,500 cases and 3,100 controls. Based on results of univariate analyses, we found borderline evidence of association for several SNPs, but no susceptibility alleles significant at the P<0.00001 level, which has been suggested as the threshold for candidate gene studies . In the current analysis, the most significant SNPs identified in this dataset were rs2107425 on chr 11p15.5 (P = 0.0012) and rs3817198 in LSP1 (P = 0.0016), both of which have been identified as susceptibility alleles for breast cancer, and rs9322336, which is located in the oestrogen receptor (ESR1) gene (P = 0.0013). All three SNPs remained significant after adjusting for population stratification.
Even though none of the associations we found were highly statistically significant, we cannot rule out that one or more of these SNPs, or alternative SNPs within the candidate genes we analysed were associated with ovarian cancer risk. The combined sample size from the three case-control studies did not have sufficient statistical power to detect associations with highly stringent levels of statistical significance. For individual variants, the statistical power of the study depends on the minor allele frequency, the risks conferred, and the genetic model. For this study, we had 97% power at the 5% significance level to detect a co-dominant allele with a minor allele frequency of 0.3 that confers an odds ratio of 1.2, and 96% power to detect a dominant allele with a minor allele frequency of 0.1 that confers an odds ratio of 1.3. For the top three SNPs, we used Pupasuite PupaSNP (http://pupasuite.bioinfo.cipf.es/)  to look for additional evidence that they may be involved in cancer aetiology, but found nothing striking. rs2107425 is located in a region of chromosome 11p15.5 which has no known genes or open reading frames; but it tags another SNP (rs2251375) with r2 = 1 that is in a region conserved in mice. rs3817198 is in the lymphocyte-specific protein 1 (LSP1) gene, also on chromosome 11p15.5, and also in a region conserved in mice. Loss of heterozygosity in this region has been found in ovarian, breast, lung, stomach and bladder cancers, and has been described as a tumour suppressor region in lung and breast cancers –. rs9322336 is in intron 2 of ESR1; no other common variants appear to tag this SNP. ESR1 is a ligand activated transcription factor, which has been implicated in ovarian and breast cancer , .
Simple multiple testing correcting methods such as the Bonferroni or Sidak are too stringent for adjusting the results for the individual SNPs. Neither of these methods take into account the correlation that exists between SNPs that tag the genetic variation across gene regions. Therefore the results reported have not been adjusted for multiple testing. However, these SNPs may not be significant after adjusting for multiple testing. We have recently shown that methods that take into account the totality of the data have greater power to detect associations . Therefore, we used the AML method to test the hypothesis that subsets of the SNPs we evaluated, rather than individual SNPs, were associated with ovarian cancer risk. We did not find evidence for an overall association between common genetic variation in the 94 candidate genes or regions and ovarian cancer risk. However we found evidence of an association for SNPs that had been identified from breast cancer association studies. This is intriguing given previous studies that have shown strong genetic links between breast and ovarian cancer for the highly the penetrant genes BRCA1 and BRCA2  and recent association studies that suggest some common variants may be low-penetrance susceptibility alleles for multiple phenotypes (i.e. pleiotropism). For example SNPs in a region of chromosome 8q24 appear to be associated with breast, prostate and ovarian cancer risk .
In conclusion, the only significant global association identified was with the breast cancer associated SNPs. However, providing definitive evidence that any of these variants represents a true susceptibility allele is limited by the sample size and consequently the statistical power to identify risk alleles for which the effect size is likely to be small. It must also be considered that many associations are not validated in a second stage study. Genome wide association studies have successfully identified common risk alleles for some common cancers, including breast and prostate cancers , . In both studies, the sample sizes were much larger than those used in the current study. These studies also showed that empirical genome-wide studies represent an efficient approach to identifying low-penetrance susceptibility alleles, but that the most significant alleles only confer small relative risks (<1.3). It will require larger numbers from more extensive multi-centre collaborations to find ovarian cancer susceptibility alleles that confer such modest risks. This has recently been achieved with the development of the ovarian cancer association consortium (OCAC), which comprises more than 20 ovarian cancer case-control studies throughout the world ; but as the experiences of the breast and prostate consortia show, common risk alleles for ovarian cancer are likely to exist and a combination of both candidate gene and genome wide association studies have the potential to identify them. However, it has so far proved difficult to identify highly significant SNPs for ovarian cancer using the candidate gene approach. GWAS studies of other cancers have shown that this is likely to prove a more successful approach to identify low-moderate risk alleles for ovarian cancer in the future.
Genotype frequencies for all SNPs in cases and controls
(0.07 MB XLS)
We thank: Joan MacIntosh, Hannah Munday, Barbara Perkins, Clare Jordan, Kristy Driver, Mitul Shah, the local general practices and nurses and the East Anglian Cancer Registry for recruitment of the Search cases: the EPIC-Norfolk investigators for recruitment of the Search controls; Craig Luccarini and Don Conroy for expert technical assistance. Finally, we would like to express our profound thanks to all the study participants who contributed to this research.
Conceived and designed the experiments: LQ JT SJR HS RAD VM EH CH JB ELG JS DFE SKK ASW SAG PDP. Performed the experiments: LQ SJR HS EW. Analyzed the data: LQ JT SJR HS EW. Contributed reagents/materials/analysis tools: RAD VM EH CH JB ELG JS DFE SKK ASW SAG PDP. Wrote the paper: LQ JT SAG PDP. Revised draft of the paper: SJR HS EW RAD VM EH CH JB ELG JS DFE SKK ASW.
- 1. Auranen A, Pukkala E, Makinen J, Sankila R, Grenman S, et al. (1996) Cancer incidence in the first-degree relatives of ovarian cancer patients. Br J Cancer 74: 280–284.A. AuranenE. PukkalaJ. MakinenR. SankilaS. Grenman1996Cancer incidence in the first-degree relatives of ovarian cancer patients.Br J Cancer74280284
- 2. Lichtenstein P, Holm NV, Verkasalo PK, Iliadou A, Kaprio J, et al. (2000) Environmental and heritable factors in the causation of cancer–analyses of cohorts of twins from Sweden, Denmark, and Finland. N Engl J Med 343: 78–85.P. LichtensteinNV HolmPK VerkasaloA. IliadouJ. Kaprio2000Environmental and heritable factors in the causation of cancer–analyses of cohorts of twins from Sweden, Denmark, and Finland.N Engl J Med3437885
- 3. Ford D, Easton DF, Stratton M, Narod S, Goldgar D, et al. (1998) Genetic heterogeneity and penetrance analysis of the BRCA1 and BRCA2 genes in breast cancer families. The Breast Cancer Linkage Consortium. Am J Hum Genet 62: 676–689.D. FordDF EastonM. StrattonS. NarodD. Goldgar1998Genetic heterogeneity and penetrance analysis of the BRCA1 and BRCA2 genes in breast cancer families. The Breast Cancer Linkage Consortium.Am J Hum Genet62676689
- 4. Ramus SJ, Harrington PA, Pye C, Dicioccio RA, Cox MJ, et al. (2007) Contribution of BRCA1 and BRCA2 mutations to inherited ovarian cancer. Hum Mutat. SJ RamusPA HarringtonC. PyeRA DicioccioMJ Cox2007Contribution of BRCA1 and BRCA2 mutations to inherited ovarian cancer.Hum Mutat
- 5. Gayther SA, Russell P, Harrington P, Antoniou AC, Easton DF, et al. (1999) The contribution of germline BRCA1 and BRCA2 mutations to familial ovarian cancer: no evidence for other ovarian cancer-susceptibility genes. Am J Hum Genet 65: 1021–1029.SA GaytherP. RussellP. HarringtonAC AntoniouDF Easton1999The contribution of germline BRCA1 and BRCA2 mutations to familial ovarian cancer: no evidence for other ovarian cancer-susceptibility genes.Am J Hum Genet6510211029
- 6. Stratton JF, Pharoah P, Smith SK, Easton D, Ponder BA (1998) A systematic review and meta-analysis of family history and risk of ovarian cancer. Br J Obstet Gynaecol 105: 493–499.JF StrattonP. PharoahSK SmithD. EastonBA Ponder1998A systematic review and meta-analysis of family history and risk of ovarian cancer.Br J Obstet Gynaecol105493499
- 7. Antoniou AC, Easton DF (2006) Risk prediction models for familial breast cancer. Future Oncol 2: 257–274.AC AntoniouDF Easton2006Risk prediction models for familial breast cancer.Future Oncol2257274
- 8. Pharoah PD, Antoniou A, Bobrow M, Zimmern RL, Easton DF, et al. (2002) Polygenic susceptibility to breast cancer and implications for prevention. Nat Genet 31: 33–36.PD PharoahA. AntoniouM. BobrowRL ZimmernDF Easton2002Polygenic susceptibility to breast cancer and implications for prevention.Nat Genet313336
- 9. Dicioccio RA, Song H, Waterfall C, Kimura MT, Nagase H, et al. (2004) STK15 polymorphisms and association with risk of invasive ovarian cancer. Cancer Epidemiol Biomarkers Prev 13: 1589–1594.RA DicioccioH. SongC. WaterfallMT KimuraH. Nagase2004STK15 polymorphisms and association with risk of invasive ovarian cancer.Cancer Epidemiol Biomarkers Prev1315891594
- 10. Auranen A, Song H, Waterfall C, Dicioccio RA, Kuschel B, et al. (2005) Polymorphisms in DNA repair genes and epithelial ovarian cancer risk. Int J Cancer 117: 611–618.A. AuranenH. SongC. WaterfallRA DicioccioB. Kuschel2005Polymorphisms in DNA repair genes and epithelial ovarian cancer risk.Int J Cancer117611618
- 11. Song H, Ramus SJ, Shadforth D, Quaye L, Kjaer SK, et al. (2006) Common variants in RB1 gene and risk of invasive ovarian cancer. Cancer Res 66: 10220–10226.H. SongSJ RamusD. ShadforthL. QuayeSK Kjaer2006Common variants in RB1 gene and risk of invasive ovarian cancer.Cancer Res661022010226
- 12. Song H, Ramus SJ, Quaye L, DiCioccio RA, Tyrer J, et al. (2006) Common variants in mismatch repair genes and risk of invasive ovarian cancer. Carcinogenesis 27: 2235–2242.H. SongSJ RamusL. QuayeRA DiCioccioJ. Tyrer2006Common variants in mismatch repair genes and risk of invasive ovarian cancer.Carcinogenesis2722352242
- 13. Song H, Ramus SJ, Kjaer SK, Hogdall E, Dicioccio RA, et al. (2007) Tagging Single Nucleotide Polymorphisms in the BRIP1 Gene and Susceptibility to Breast and Ovarian Cancer. PLoS ONE 2: e268.H. SongSJ RamusSK KjaerE. HogdallRA Dicioccio2007Tagging Single Nucleotide Polymorphisms in the BRIP1 Gene and Susceptibility to Breast and Ovarian Cancer.PLoS ONE2e268
- 14. Gayther SA, Song H, Ramus SJ, Kjaer SK, Whittemore AS, et al. (2007) Tagging single nucleotide polymorphisms in cell cycle control genes and susceptibility to invasive epithelial ovarian cancer. Cancer Res 67: 3027–3035.SA GaytherH. SongSJ RamusSK KjaerAS Whittemore2007Tagging single nucleotide polymorphisms in cell cycle control genes and susceptibility to invasive epithelial ovarian cancer.Cancer Res6730273035
- 15. Pearce CL, Wu AH, Gayther SA, Bale AE, Beck PA, et al. (2008) Progesterone receptor variation and risk of ovarian cancer is limited to the invasive endometrioid subtype: results from the Ovarian Cancer Association Consortium pooled analysis. Br J Cancer 98: 282–288.CL PearceAH WuSA GaytherAE BalePA Beck2008Progesterone receptor variation and risk of ovarian cancer is limited to the invasive endometrioid subtype: results from the Ovarian Cancer Association Consortium pooled analysis.Br J Cancer98282288
- 16. Ramus SJ, Vierkant RA, Johnatty SE, Pike MC, Van Den Berg DJ, et al. (2008) Consortium analysis of 7 candidate SNPs for ovarian cancer. Int J Cancer 123: 380–388.SJ RamusRA VierkantSE JohnattyMC PikeDJ Van Den Berg2008Consortium analysis of 7 candidate SNPs for ovarian cancer.Int J Cancer123380388
- 17. Palmieri RT, Wilson MA, Iversen ES, Clyde MA, Calingaert B, et al. (2008) Polymorphism in the IL18 gene and epithelial ovarian cancer in non-Hispanic white women. Cancer Epidemiol Biomarkers Prev 17: 3567–3572.RT PalmieriMA WilsonES IversenMA ClydeB. Calingaert2008Polymorphism in the IL18 gene and epithelial ovarian cancer in non-Hispanic white women.Cancer Epidemiol Biomarkers Prev1735673572
- 18. Quaye L, Song H, Ramus SJ, Gentry-Maharaj A, Hogdall E, et al. (2009) Tagging single nucleotide polymorphisms in candidate oncogenes and susceptibility to ovarian cancer. Br J Cancer In press. L. QuayeH. SongSJ RamusA. Gentry-MaharajE. Hogdall2009Tagging single nucleotide polymorphisms in candidate oncogenes and susceptibility to ovarian cancer.Br J Cancer In press
- 19. Song H, Ramus SJ, Krüger Kjaer S, DiCioccio RA, Chenevix-Trench G, et al. (Submitted) Association between invasive ovarian cancer susceptibility and 11 candidate SNPs from a breast cancer genome-wide association study. Hum Mol Genet. H. SongSJ RamusS. Krüger KjaerRA DiCioccioG. Chenevix-Trench(Submitted) Association between invasive ovarian cancer susceptibility and 11 candidate SNPs from a breast cancer genome-wide association study.Hum Mol Genet
- 20. Easton DF, Pooley KA, Dunning AM, Pharoah PD, Thompson D, et al. (2007) Genome-wide association study identifies novel breast cancer susceptibility loci. Nature 447: 1087–1093.DF EastonKA PooleyAM DunningPD PharoahD. Thompson2007Genome-wide association study identifies novel breast cancer susceptibility loci.Nature44710871093
- 21. Hunter DJ, Kraft P, Jacobs KB, Cox DG, Yeager M, et al. (2007) A genome-wide association study identifies alleles in FGFR2 associated with risk of sporadic postmenopausal breast cancer. Nat Genet 39: 870–874.DJ HunterP. KraftKB JacobsDG CoxM. Yeager2007A genome-wide association study identifies alleles in FGFR2 associated with risk of sporadic postmenopausal breast cancer.Nat Genet39870874
- 22. Tomlinson IP, Webb E, Carvajal-Carmona L, Broderick P, Howarth K, et al. (2008) A genome-wide association study identifies colorectal cancer susceptibility loci on chromosomes 10p14 and 8q23.3. Nat Genet 40: 623–630.IP TomlinsonE. WebbL. Carvajal-CarmonaP. BroderickK. Howarth2008A genome-wide association study identifies colorectal cancer susceptibility loci on chromosomes 10p14 and 8q23.3.Nat Genet40623630
- 23. Tenesa A, Farrington SM, Prendergast JG, Porteous ME, Walker M, et al. (2008) Genome-wide association scan identifies a colorectal cancer susceptibility locus on 11q23 and replicates risk loci at 8q24 and 18q21. Nat Genet 40: 631–637.A. TenesaSM FarringtonJG PrendergastME PorteousM. Walker2008Genome-wide association scan identifies a colorectal cancer susceptibility locus on 11q23 and replicates risk loci at 8q24 and 18q21.Nat Genet40631637
- 24. Gudmundsson J, Sulem P, Rafnar T, Bergthorsson JT, Manolescu A, et al. (2008) Common sequence variants on 2p15 and Xp11.22 confer susceptibility to prostate cancer. Nat Genet 40: 281–283.J. GudmundssonP. SulemT. RafnarJT BergthorssonA. Manolescu2008Common sequence variants on 2p15 and Xp11.22 confer susceptibility to prostate cancer.Nat Genet40281283
- 25. Eeles RA, Kote-Jarai Z, Giles GG, Olama AA, Guy M, et al. (2008) Multiple newly identified loci associated with prostate cancer susceptibility. Nat Genet 40: 316–321.RA EelesZ. Kote-JaraiGG GilesAA OlamaM. Guy2008Multiple newly identified loci associated with prostate cancer susceptibility.Nat Genet40316321
- 26. Simes RJ (1986) An improved Bonferroni procedure for multiple tests of significance. Biometrika 73: 751–754.RJ Simes1986An improved Bonferroni procedure for multiple tests of significance.Biometrika73751754
- 27. Hoh J, Wille A, Ott J (2001) Trimming, weighting, and grouping SNPs in human case-control association studies. Genome Res 11: 2115–2119.J. HohA. WilleJ. Ott2001Trimming, weighting, and grouping SNPs in human case-control association studies.Genome Res1121152119
- 28. Zaykin DV, Zhivotovsky LA, Westfall PH, Weir BS (2002) Truncated product method for combining P-values. Genet Epidemiol 22: 170–185.DV ZaykinLA ZhivotovskyPH WestfallBS Weir2002Truncated product method for combining P-values.Genet Epidemiol22170185
- 29. Dudbridge F, Koeleman BP (2003) Rank truncated product of P-values, with application to genomewide association scans. Genet Epidemiol 25: 360–366.F. DudbridgeBP Koeleman2003Rank truncated product of P-values, with application to genomewide association scans.Genet Epidemiol25360366
- 30. Schaid DJ, McDonnell SK, Hebbring SJ, Cunningham JM, Thibodeau SN (2005) Nonparametric tests of association of multiple genes with human disease. Am J Hum Genet 76: 780–793.DJ SchaidSK McDonnellSJ HebbringJM CunninghamSN Thibodeau2005Nonparametric tests of association of multiple genes with human disease.Am J Hum Genet76780793
- 31. Tyrer J, Pharoah PD, Easton DF (2006) The admixture maximum likelihood test: a novel experiment-wise test of association between disease and multiple SNPs. Genet Epidemiol 30: 636–643.J. TyrerPD PharoahDF Easton2006The admixture maximum likelihood test: a novel experiment-wise test of association between disease and multiple SNPs.Genet Epidemiol30636643
- 32. Pharoah PD, Tyrer J, Dunning AM, Easton DF, Ponder BA (2007) Association between common variation in 120 candidate genes and breast cancer risk. PLoS Genet 3: e42.PD PharoahJ. TyrerAM DunningDF EastonBA Ponder2007Association between common variation in 120 candidate genes and breast cancer risk.PLoS Genet3e42
- 33. Devlin B, Roeder K, Bacanu SA (2001) Unbiased methods for population-based association studies. Genet Epidemiol 21: 273–284.B. DevlinK. RoederSA Bacanu2001Unbiased methods for population-based association studies.Genet Epidemiol21273284
- 34. Baxter SW, Thomas EJ, Campbell IG (2001) GSTM1 null polymorphism and susceptibility to endometriosis and ovarian cancer. Carcinogenesis 22: 63–65.SW BaxterEJ ThomasIG Campbell2001GSTM1 null polymorphism and susceptibility to endometriosis and ovarian cancer.Carcinogenesis226365
- 35. Aktas D, Guney I, Alikasifoglu M, Yuce K, Tuncbilek E, et al. (2002) CYP1A1 gene polymorphism and risk of epithelial ovarian neoplasm. Gynecol Oncol 86: 124–128.D. AktasI. GuneyM. AlikasifogluK. YuceE. Tuncbilek2002CYP1A1 gene polymorphism and risk of epithelial ovarian neoplasm.Gynecol Oncol86124128
- 36. Thomas DC, Haile RW, Duggan D (2005) Recent developments in genomewide association scans: a workshop summary and review. Am J Hum Genet 77: 337–345.DC ThomasRW HaileD. Duggan2005Recent developments in genomewide association scans: a workshop summary and review.Am J Hum Genet77337345
- 37. Conde L, Vaquerizas JM, Dopazo H, Arbiza L, Reumers J, et al. (2006) PupaSuite: finding functional single nucleotide polymorphisms for large-scale genotyping purposes. Nucleic Acids Res 34: W621–625.L. CondeJM VaquerizasH. DopazoL. ArbizaJ. Reumers2006PupaSuite: finding functional single nucleotide polymorphisms for large-scale genotyping purposes.Nucleic Acids Res34W621625
- 38. Karnik P, Paris M, Williams BR, Casey G, Crowe J, et al. (1998) Two distinct tumor suppressor loci within chromosome 11p15 implicated in breast cancer progression and metastasis. Hum Mol Genet 7: 895–903.P. KarnikM. ParisBR WilliamsG. CaseyJ. Crowe1998Two distinct tumor suppressor loci within chromosome 11p15 implicated in breast cancer progression and metastasis.Hum Mol Genet7895903
- 39. Xu XL, Wu LC, Du F, Davis A, Peyton M, et al. (2001) Inactivation of human SRBC, located within the 11p15.5-p15.4 tumor suppressor region, in breast and lung cancers. Cancer Res 61: 7943–7949.XL XuLC WuF. DuA. DavisM. Peyton2001Inactivation of human SRBC, located within the 11p15.5-p15.4 tumor suppressor region, in breast and lung cancers.Cancer Res6179437949
- 40. Gudmundsson J, Barkardottir RB, Eiriksdottir G, Baldursson T, Arason A, et al. (1995) Loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations. Br J Cancer 72: 696–701.J. GudmundssonRB BarkardottirG. EiriksdottirT. BaldurssonA. Arason1995Loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations.Br J Cancer72696701
- 41. Bepler G, Fong KM, Johnson BE, O'Briant KC, Daly LA, et al. (1998) Association of chromosome 11 locus D11S12 with histology, stage, and metastases in lung cancer. Cancer Detect Prev 22: 14–19.G. BeplerKM FongBE JohnsonKC O'BriantLA Daly1998Association of chromosome 11 locus D11S12 with histology, stage, and metastases in lung cancer.Cancer Detect Prev221419
- 42. Viel A, Giannini F, Tumiotto L, Sopracordevole F, Visentin MC, et al. (1992) Chromosomal localisation of two putative 11p oncosuppressor genes involved in human ovarian tumours. Br J Cancer 66: 1030–1036.A. VielF. GianniniL. TumiottoF. SopracordevoleMC Visentin1992Chromosomal localisation of two putative 11p oncosuppressor genes involved in human ovarian tumours.Br J Cancer6610301036
- 43. Shaw ME, Knowles MA (1995) Deletion mapping of chromosome 11 in carcinoma of the bladder. Genes Chromosomes Cancer 13: 1–8.ME ShawMA Knowles1995Deletion mapping of chromosome 11 in carcinoma of the bladder.Genes Chromosomes Cancer1318
- 44. Baffa R, Negrini M, Mandes B, Rugge M, Ranzani GN, et al. (1996) Loss of heterozygosity for chromosome 11 in adenocarcinoma of the stomach. Cancer Res 56: 268–272.R. BaffaM. NegriniB. MandesM. RuggeGN Ranzani1996Loss of heterozygosity for chromosome 11 in adenocarcinoma of the stomach.Cancer Res56268272
- 45. Imura M, Yamashita S, Cai LY, Furuta J, Wakabayashi M, et al. (2006) Methylation and expression analysis of 15 genes and three normally-methylated genes in 13 Ovarian cancer cell lines. Cancer Lett 241: 213–220.M. ImuraS. YamashitaLY CaiJ. FurutaM. Wakabayashi2006Methylation and expression analysis of 15 genes and three normally-methylated genes in 13 Ovarian cancer cell lines.Cancer Lett241213220
- 46. Dunning AM, Healey CS, Baynes C, Maia AT, Scollen S, et al. (2009) Association of ESR1 gene tagging SNPs with breast cancer risk. Hum Mol Genet 18: 1131–1139.AM DunningCS HealeyC. BaynesAT MaiaS. Scollen2009Association of ESR1 gene tagging SNPs with breast cancer risk.Hum Mol Genet1811311139
- 47. Ghoussaini M, Song H, Koessler T, Al Olama AA, Kote-Jarai Z, et al. (2008) Multiple loci with different cancer specificities within the 8q24 gene desert. J Natl Cancer Inst 100: 962–966.M. GhoussainiH. SongT. KoesslerAA Al OlamaZ. Kote-Jarai2008Multiple loci with different cancer specificities within the 8q24 gene desert.J Natl Cancer Inst100962966
- 48. Yeager M, Orr N, Hayes RB, Jacobs KB, Kraft P, et al. (2007) Genome-wide association study of prostate cancer identifies a second risk locus at 8q24. Nat Genet 39: 645–649.M. YeagerN. OrrRB HayesKB JacobsP. Kraft2007Genome-wide association study of prostate cancer identifies a second risk locus at 8q24.Nat Genet39645649
- 49. Berchuck A, Schildkraut JM, Pearce CL, Chenevix-Trench G, Pharoah PD (2008) Role of genetic polymorphisms in ovarian cancer susceptibility: development of an international ovarian cancer association consortium. Adv Exp Med Biol 622: 53–67.A. BerchuckJM SchildkrautCL PearceG. Chenevix-TrenchPD Pharoah2008Role of genetic polymorphisms in ovarian cancer susceptibility: development of an international ovarian cancer association consortium.Adv Exp Med Biol6225367