SUMO is a protein modifier that is vital for multicellular development. Here we present the first system-wide analysis, combining multiple approaches, to correlate the sumoylated proteome (SUMO-ome) in a multicellular organism with the developmental roles of SUMO. Using mass-spectrometry-based protein identification, we found over 140 largely novel SUMO conjugates in the early Drosophila embryo. Enriched functional groups include proteins involved in Ras signaling, cell cycle, and pattern formation. In support of the functional significance of these findings, sumo germline clone embryos exhibited phenotypes indicative of defects in these same three processes. Our cell culture and immunolocalization studies further substantiate roles for SUMO in Ras signaling and cell cycle regulation. For example, we found that SUMO is required for efficient Ras-mediated MAP kinase activation upstream or at the level of Ras activation. We further found that SUMO is dynamically localized during mitosis to the condensed chromosomes, and later also to the midbody. Polo kinase, a SUMO substrate found in our screen, partially colocalizes with SUMO at both sites. These studies show that SUMO coordinates multiple regulatory processes during oogenesis and early embryogenesis. In addition, our database of sumoylated proteins provides a valuable resource for those studying the roles of SUMO in development.
Citation: Nie M, Xie Y, Loo JA, Courey AJ (2009) Genetic and Proteomic Evidence for Roles of Drosophila SUMO in Cell Cycle Control, Ras Signaling, and Early Pattern Formation. PLoS ONE 4(6): e5905. https://doi.org/10.1371/journal.pone.0005905
Editor: Magnus Holm, Gothenburg University, Sweden
Received: April 7, 2009; Accepted: May 17, 2009; Published: June 16, 2009
Copyright: © 2009 Nie et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The UCLA Mass Spectrometry and Proteomics Technology Center was established with a grant from the W. M. Keck Foundation. This work was supported by an NIH (http://www.nih.gov/) grant (GM63596) to A.J.C. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Post-translational protein modification adds layers of complexity to macromolecular function. One way of modifying proteins is by joining the ubiquitin family proteins to lysine residues, generating branched proteins . One such ubiquitin-like protein, SUMO (small ubiquitin-related modifier), displays remarkable versatility in modulating target protein function. Many proteins are targeted for covalent modification by SUMO, which consequently modulates many cellular processes –.
Genetic analysis has revealed essential roles for SUMO in the survival and development of organisms ranging in complexity from yeast to mammals –. In S. cerevisiae, mutations in genes encoding SUMO pathway enzymes are lethal –, while mutations in the corresponding genes in S. pombe severely impair growth –. Deletion of genes encoding enzymes required for SUMO conjugation in C. elegans leads to embryonic lethality , while reduction of the SUMO conjugating enzyme levels in Drosophila, zebrafish, and mouse results in developmental defects –.
The Drosophila melanogaster genome encodes a single form of SUMO (herein referred to as Drosophila SUMO, but also known as Drosophila Smt3), which shares 52% and 73% sequence identity with human SUMO-1 and SUMO-2, respectively . Drosophila and human SUMO family proteins are at least partially interchangeable, demonstrating a high level of SUMO pathway conservation between evolutionarily distant organisms . To date, only a few Drosophila proteins, such as the transcription factors Dorsal , , Tramtrack , Vestigial , SoxNeuro , and Medea ; the gypsy insulator interacting proteins Mod(mdg4) and CP190 ; as well as the bi-functional tRNA charging enzyme glutamylprolyl-tRNA synthetase (EPRS, ) are known to be sumoylated. SUMO appears to have diverse roles in the Drosophila life cycle, including the regulation of transcription and the modulation of the immune response , .
While SUMO is present throughout development, early Drosophila embryos contain particularly high concentrations of maternally contributed SUMO and the enzymes required for SUMO conjugation , , , suggesting that sumoylation may play particularly critical roles at this stage of fly development. Previous global analyses of SUMO substrates in S. cerevisiae and mammalian cultured cells have produced extensive lists of novel sumoylation targets –. To date, however, there are no published studies that document the spectrum of sumoylated proteins in a specific developmental setting in a multicellular organism.
To broaden our understanding of the function of sumoylation in early Drosophila development, we performed a mass spectrometry-based global identification of sumoylation targets in early embryos, and found over 140 direct sumoylation targets. Among the identified SUMO target proteins are players in many processes essential to embryonic development, including proteins involved in Ras signaling, cell cycle control, and embryonic patterning. To determine the functional significance of the identified sumoylated proteins, we carried out genetic, cell culture and immunolocalization studies, obtaining evidence for roles of SUMO in these same three processes. Thus, the proteomic, genetic, and cellular studies presented here all converge to suggest that SUMO coordinates key aspects of early metazoan development.
Isolation, identification, and categorization of early embryonic SUMO conjugates
To determine the early embryonic SUMO-ome (catalog of sumoylated proteins), we adopted a scheme that involved a two-step affinity purification strategy using SUMO tagged at its N-terminus with both (His)6 and FLAG tags, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based protein identification of trypsin-digested proteins (Figure 1A). We initially attempted to express tagged SUMO using modified sumo genomic clones, but were unsuccessful presumably due to the need for unknown distant cis-regulatory modules to direct sumo expression. We therefore turned to the Gal4-UAS system , and drove ubiquitous maternal expression of tagged SUMO at levels slightly lower than that of endogenous SUMO (Figure S1). Tagged SUMO rescues the lethality resulting from sumo mutations (data not shown) demonstrating the functionality of the tagged protein.
A) Scheme for identifying Drosophila embryonic SUMO conjugates. SUMO conjugates were isolated by tandem affinity purification from transgenic fly embryos expressing (His)6-FLAG-SUMO. The initial purification step (Ni-NTA chromatography) was performed under denaturing conditions. To maximize the chance of detecting low abundance proteins in the complex protein mixture, the affinity-purified proteins were separated by SDS-PAGE, and the lane was cut into 20 evenly divided gel slices. Tryptic peptides extracted from each gel slice were analyzed by LC-MS/MS. B) A bacterial sumoylation assay. The QSUMO vector, which encodes the mature form of SUMO (SUMOGG) along with SAE1, SAE2, and Ubc9 expressed from separate T7/lac promoters, was used in combination with a vector expressing a GST-tagged candidate substrate. As a negative control, QΔGG, which expresses a conjugation defective form of SUMO (SUMOΔGG), was used in place of QSUMO. C) Bacterial sumoylation assays were used to validate proteins identified in the proteomic screen as sumoylation substrates. GST-tagged candidate SUMO conjugates were expressed in BL21 cells co-transformed with QSUMO or QΔGG vectors, purified using glutathione beads, and immunoblotted using antibodies against GST, SUMO, or poly-His (to detect 6xHis-tagged SUMO). GST by itself was not sumoylated in this assay. Black arrows point to the bands representing sumoylated proteins, and open arrow points to a non-specific reacting band. D) The eIF4E protein was purified from Drosophila S2 cells stably expressing FLAG-(His)6- tagged eIF4E using Ni-NTA beads under denaturing conditions. The resulting proteins were probed with anti-FLAG antibody in a Western blot. The cells were treated with SUMO or control YFP dsRNA for 3 days prior to cell lysis. In the control sample, the bands representing the sumoylated species (black arrows) have intensities that are 8.1% (top) and 12.9% (bottom) of the intensity of the band representing unmodified eIF4E (∼40 kDa), whereas in the SUMO knockdown sample, they are reduced to 1.8% (top) and 3.5% (bottom). Quantitation was performed using Quantity One 4.3.0 (BioRad).
In other organisms and cultured cells, heat shock is known to enhance global sumoylation , . We observed a similar phenomenon in early embryos (Figure S1). Since the SUMO conjugates were in low abundance even when very large amount of starting materials (five grams of fly embryos) were used, we promoted SUMO conjugation with heat treatment at 37°C. As a control, wild-type Oregon-R (Ore-R) embryos lacking tagged SUMO were collected and processed under identical conditions. While the use of heat shock could raise a concern about the possibility of skewing the protein pool, our pilot study in which proteins were isolated from heat-shocked and non-heat-shocked samples revealed similar SDS-PAGE profiles (Figure S2). Furthermore, analysis by LC-MS/MS revealed a largely overlapping set of proteins from heat-shocked and non-heat-shocked embryos (supporting document S1 and Table S3). The observed differences were largely quantitative rather than qualitative (Table S3)–more peptides were identified from the majority of the proteins in the heat-shocked than in the non-heat-shocked samples, leading to higher confidence protein identification. Moreover, the consistency between our phenotypic analysis (see below) and our proteomic data further increases our confidence in the biological relevance of our SUMO-ome.
In the first step of the two-step affinity purification, the (His)6 tag was bound to nickel-coupled agarose under strongly denaturing conditions (containing 1% CHAPS, 8 M urea) in order to solubilize proteins from all cellular compartments, suppress SUMO isopeptidases, and ensure that the purified proteins are directly conjugated to SUMO. To achieve a higher degree of purification, second affinity chromatography step employing anti-FLAG antibodies was carried out. Sypro Ruby staining of a gel with proteins purified from embryos containing dual-tagged SUMO and from negative control embryos, demonstrated very few proteins in the control sample (Figure 1A). The SDS-PAGE gel lanes containing the control and experimental samples were each cut into 20 equal-size slices (Figure 1A), followed by in-gel tryptic digestion and subsequent analysis by LC-MS/MS.
LC-MS/MS data were analyzed using Mascot (Matrix Science) to search the database of known Drosophila protein sequences. For proteins represented by four or fewer peptides, we manually inspected the mass spectra to confirm the protein identifications. SUMO tryptic peptides were detected in every gel slice from the sample prepared from embryos expressing tagged-SUMO, and in none of the gel slices from the sample prepared from control Ore-R embryos. A total of 144 proteins (corresponding to 142 genes) were uniquely found in the sample from embryos expressing tagged-SUMO (Table S1). In addition to a large number of novel sumoylation substrates identified, this list includes nearly all previously validated Drosophila SUMO conjugates that are present at this developmental stage (e.g., Dorsal, Mod(mdg4), CaMKII, EPRS), as well as proteins that are orthologous to well-characterized SUMO substrates found in other organisms (e.g., PCNA, CtBP, Topoisomerase I and II), thus adding to our confidence in the authenticity of our SUMO-omic database.
In a separate study, tagged SUMO conjugates were isolated under native conditions in a single-step anti-FLAG immunopurification, and over 300 gene products were identified (Table S2). This set includes a large fraction of the proteins (∼60%) identified in the two-step purification procedure (marked by “*” in Table S1). The native purification also appears to have isolated a number of proteins that interact with the SUMO conjugates identified in the two-step purification carried out under denaturing conditions, as well as proteins that were later demonstrated to be authentic SUMO conjugation targets by independent validation methods (see below).
We used a SUMO conjugation site prediction algorithm, SUMOsp , to analyze the proteins identified in the one- and two-step purifications (Table 1). While the proteins in the entire Drosophila proteome contain an average of 0.76 consensus SUMO conjugation sites per protein, the proteins from the two-step purification average 1.46 consensus sites per protein, and the proteins from the one-step purification average 1.06 consensus sites per protein. These differences are highly statistically significant (Table 1). The lower number of sites in the proteins from the one-step purification relative to the two-step purification further supports the idea that the one-step purification yielded a mixture of direct SUMO conjugation targets and their interacting partners.
We used the Generic Gene Ontology (GO) Term finder tool (http://go.princeton.edu/cgi-bin/GOTermMapper) to search for overrepresented GO categories in the SUMO substrate list when compared to the entire fly proteome. Using the hypergeometric distribution analysis, we calculated the probability that the proportion of SUMO substrates mapped to a given category could occur by random chance, given the fraction of all fly proteins that map to that category (Table S4, S5). This analysis helped us to distinguish several enriched functional groups of SUMO conjugates (Table 2). We also analyzed the enrichment of biological process GO terms in our SUMO proteome when compared to the early Drosophila embryonic proteome (2 hr AEL embryo , ) (Table S6). Many of the same categories that are enriched in comparison to the entire Drosophila proteome are also significantly enriched in comparison to the early embryonic proteome.
Inspection of the list of sumoylated proteins also suggests that protein complexes, such as the oskar mRNP, multi-aminoacyl-tRNA synthetase complex, a PCNA-containing protein complex, and the ribosome, often contain multiple sumoylated proteins (Table 3).
Validation of SUMO conjugates found through global MS analysis
Using a bacterial sumoylation assay we validated a number of the proteins in the enriched groups as SUMO conjugation targets (Figure 1B). For this assay, we generated E. coli expressing the four Drosophila polypeptides essential for SUMO conjugation (SAE1, SAE2, Ubc9, and SUMO) as well as a GST-tagged candidate SUMO substrate. The detection of SUMO conjugates in this system is facilitated by the lack of an absolute requirement for E3-type ligases in sumoylation ,  as well as by the absence of SUMO deconjugating enzymes in bacteria. While bacterial sumoylation systems may not completely recapitulate the specificity of sumoylation in vivo, they have been repeatedly validated as a useful approach for confirming sumoylation targets –.
Our bacterial sumoylation system includes several improvements over the existing bacterial systems. We use a single vector (QSUMO) to encode all four polypeptides required for sumoylation, thereby reducing variation in expression levels. In addition, we incorporated a loxP site into a GST fusion protein expression vector to enable high-throughput cloning of cDNAs encoding potential sumoylation targets. To provide a control for specificity, we generated a control vector, QΔGG, which encodes a conjugation defective form of SUMO lacking the C-terminal di-glycine motif required for SUMO conjugation , .
Using this system we confirmed sumoylation of PCNA, the processivity factor for DNA polymerase δ, and RFC2 (Figure 1C), a subunit of the factor that loads PCNA onto DNA. We also verified sumoylation of 14-3-3 ζ (Figure 1C), which belongs to a family of small proteins that interact with a multitude of functionally diverse signaling proteins by binding to phosphorylated serine or threonine residues . Drosophila 14-3-3 ζ has been shown to function both in the Ras/MAPK pathway and in regulation of the nuclear cleavage cycles in the syncytial embryo . Using the same assay system, we also confirmed that EPRS, CtBP, eIF4E (Figure 1C), Squid (Figure S4B), and several other proteins found in our MS identification (proteins underlined in Table 2) are sumoylation substrates. We also showed that eIF4E is sumoylated in S2 cell culture, further confirming it as a genuine SUMO substrate (Figure 1D).
This bacterial sumoylation system does not non-specifically sumoylate any substrate as shown by the negative controls. In addition to GST, we also did not detect sumoylation of GFP or HP1 (Figure S4A). This latter protein was identified in the single-step native purification, but not through the two-step protocol that involved initial denaturation of the extract. This suggests that HP1 interacts with sumoylated proteins, but is not itself a sumoylation substrate. In addition, using this assay, we have been able to map specific sumoylation sites in a number of proteins including EPRS, Grauzone, Meics (data not shown), and Ras1 (see below).
Maternally contributed SUMO is required for embryonic development
Among the enriched functional groups from the proteomic screen (Table 2) are groups of proteins with functions in embryonic pattern formation, including transcription factors, such as Dorsal, Bicoid, and Hunchback, that guide the dorsoventral or anteroposterior patterning of the embryo, as well as proteins involved in the localization and translational regulation of important maternal transcripts. We also found significant enrichment of proteins involved in cell cycle regulation and the Ras signaling pathway. To determine if these enriched functional groups reflect the roles of SUMO in Drosophila embryonic development, we carried out genetic and phenotypic analysis of flies carrying hypomorphic sumo alleles.
Two independent P-insertions sumo alleles, termed sumo04493 and sumok01211, were subjected to phenotypic analysis. The P-insertion in both alleles is 20 bp upstream of the transcription start site, creating recessive lethal mutations with a lethal period before or during the early second larval instar. Evaluation of mRNA levels by RT-PCR in homozygous mutant larvae shows that the sumo04493 mutation leads to an approximately 5-fold decrease in the level of sumo transcripts (Figure S3). Antibody staining of mutant follicle cell clones (see below) also demonstrates a significant reduction in SUMO levels in mutant tissue (Figure 2A). sumo04493, sumok01211, and a sumo EMS allele (generated by Shanti Chandrashekaran, New Delhi, and obtained through Dr. Lawrence Marsh), which contains a serine-glycine sequence in place of the normal C-terminal di-glycine motif required for efficient conjugation of SUMO to its targets ,  fail to complement one another.
A) Follicle cell clones in the sumo04493 GLC egg chambers. Wild-type (Ore-R; top) and sumo04493 GLC egg chambers (bottom) were stained with SUMO antibodies (green) and DAPI (blue). The field in the lower panels contains a sumo04493/04493 clone resulting from FLP/FRT recombination (yellow arrows), heterozygous sumo04493/+ cells with a reduced level of SUMO (white arrows), and a sumo+/+ twin spot containing a level of SUMO comparable to that observed in Ore-R follicle cells (red arrows). B) A variety of anteroposterior (3 panels on the left) and dorsoventral (panel on the right) patterning defects were observed in sumo04493 mutant GLC embryos. Among the embryos that formed cuticles, 59% (n = 171) exhibited abnormal cuticular morphology. Top panel shows a wild type cuticle. C) Examples of ventralized eggshells of sumo04493 GLC embryos are shown in the three panels on the right. A wild type Ore-R embryo is shown in the leftmost panel.
To determine the function of maternally contributed SUMO, we created females containing homozygous sumo mutant germline clones (GLCs) using the hsFLP/FRT dominant female sterile method . The two P-insertion alleles of sumo exhibited overlapping spectra of defects, with the average severity of the defects being greater in the sumok01211 GLC embryos (Table 4). Since the sumok01211 GLC embryo-producing females laid relatively few mature eggs, most of the subsequent phenotypic analysis was carried out using the sumo04493 GLC embryos.
Less than 30% of the sumo04493 GLC embryos hatched, and all hatched larvae died during the first larval instar. Greater than 90% of the unhatched embryos died prior to cuticle formation. The embryos that deposited cuticle exhibited a wide range of both anteroposterior and dorsoventral patterning defects (Figure 2B). These defects are not rescued by zygotic SUMO expression since embryos produced by GLC females mated with wild-type males had similar high rates of lethality and a comparable spectrum of defects as those embryos produced by GLC females mated with heterozygous males. Both the patterning defects in the sumo mutant GLC embryos and the large number of SUMO targets from our proteomic analysis with roles in embryonic pattern formation support the conclusion that SUMO modulates the activities of key pattern formation gene products to help direct embryonic development.
SUMO plays roles in eggshell patterning and potentiates Ras/MAPK signaling
Approximately ten percent (39 out of 400) of the sumo04493 GLC eggs exhibited partially to fully fused dorsal appendages, indicative of weak to moderate eggshell ventralization (Figure 2C). Drosophila eggshell patterning is regulated by the epidermal growth factor receptor (EGFR) signaling pathway. EGFR, a transmembrane receptor tyrosine kinase (RTK), is found in the follicle cells where it receives a spatially localized signal from the developing oocyte. This signal activates the Ras signaling cascade, which patterns the follicle cell epithelium, and is therefore essential for proper patterning of the eggshell –. Both sumo04493 and sumok01211 have been shown to enhance the weakly ventralized eggshell phenotypes of a hypomorphic Ras1 mutant . Our observation that the sumo mutation leads to eggshell ventralization even in a wild-type Ras1 background further supports a role for SUMO in EGFR/Ras signaling.
Previous epistasis studies showed that reduced SUMO levels suppressed the eggshell dorsalization resulting from a constitutively active form of EGFR, indicating that SUMO acts downstream of EGFR in the follicle cells . Since the process of generating GLC also leads to production of somatic clones in the follicle cells (Figure 2A), our findings are consistent with a role for SUMO downstream of EGFR in eggshell patterning. Intriguingly, our proteomic analysis also found multiple proteins involved in Ras signaling downstream of EGFR activation as potential SUMO targets (Table 2). Further evidence that SUMO functions downstream of EGFR is provided by experiments described below in which we examine the effect of SUMO knockdown on Ras signaling in S2 cells.
To determine if SUMO plays a role in Ras signaling, we knocked down SUMO by RNAi in cultured S2R+ cells, which express Drosophila EGFR , and examined the level of MAPK activation in these cells upon activation of EGFR by the secreted Spitz ligand, sSpi . Anti-SUMO immunoblotting showed that the treatment with SUMO dsRNA progressively decreased the levels of both free SUMO and SUMO conjugates after 3 to 5 days of treatment (Figure 3A). The level of Ras pathway activation was assessed by immunoblotting for pMAPK, and all samples were normalized by comparison to total MAPK using an antibody that recognizes all forms of MAPK. The levels of pMAPK decreased with increasing duration of SUMO RNAi up to five days, and parallel treatment of cells with control YFP dsRNA had no effect on MAPK activation (Figure 3B). SUMO RNAi similarly impaired insulin-induced MAPK phosphorylation (Figure 3C). Insulin or sSpi-induced MEK activation was also reduced by SUMO knockdown (Figure 3D, normalized using the total MEK levels). To further dissect the role of SUMO in Ras signaling, we examined the requirement for SUMO in pathway activation by RasV12, a constitutively active form of Ras1. SUMO knockdown did not affect RasV12-induced MAPK activation (Figure 3E). This suggests that SUMO knock down does not impair the processes downstream of Ras1 activation, so SUMO pathway affects a step upstream of Ras1 or at the level of Ras1 to activate the pathway.
In panels A to E, lysates from equal number of cells were loaded onto SDS-PAGE. A) Anti-SUMO immunoblot of total protein from cells treated with SUMO or control YFP dsRNA. B, C) EGFR expressing S2R+ cells were treated with YFP or SUMO dsRNA for 3 to 5 days. At the end of the RNAi treatment, 2×106 cells were incubated in serum-free medium for one hour, then exposed to secreted Spitz (sSpi) ligand or insulin for 7 min and immediately lysed for Western analysis. The blots were probed with antibodies against double-phosphorylated MAPK (pMAPK) or total MAPK in B and in left half of C. In the right half of panel C, S2 cells treated with dsRNA for 5 days were stimulated with insulin, and the same samples were probed with antibodies against α-Tubulin, pMAPK, or SUMO. D) An immunoblot of cells treated with YFP or SUMO dsRNA, and exposed to sSpi or insulin, was probed with antibodies against phosphorylated MEK (pMEK) or total MEK. E) An immunoblot of RasV12-expressing S2 cells treated with YFP or SUMO dsRNA was probed with antibodies against pMAPK or total MAPK. RasV12 expression was induced with copper during the last 18 hr of dsRNA treatment. F) Bacterial sumoylation of GST-Ras1 and Ras1K104R using the approach described in the legend to Figure 1. G) Bacterial sumoylation of GST-Ras11–164, Ras17KR, or Ras165–189. In the lower panel (GST-Ras165–189), the black arrowheads point to the bands representing sumoylated proteins. Based on size, these are likely the mono-, di-, and tri-sumoylated species. The di- and tri-sumoylated species are only visible in the anti-SUMO immunoblot as the anti-GST antibody is not sensitive enough to detect them. The open arrowhead marks a non-specific cross-reacting band detected by the anti-GST antibody.
Ras1 was identified in the single-step native FLAG IP of sumoylated proteins (Table 2, Table S2), but not in the two-step denaturing purification protocol. However, the bacterial sumoylation assay suggests that it is nonetheless a direct sumoylation target. Sumoylated GST-Ras1 species were detected when the functional sumoylation pathway (QSUMO) was co-expressed with GST-Ras1 in E. coli (Figure 3F), and absent when conjugation defective SUMOΔGG (QΔGG) was employed instead (data not shown). There is no consensus SUMO acceptor site found in the Ras1 peptide sequence. The highest probability non-consensus site, predicted by both SUMOsp2.0  and SUMOplot™, is lysine 104. Mutation of lysine 104 to arginine, did not dramatically compromise Ras1 sumoylation (Figure 3F), suggesting that Ras1 contains multiple non-consensus sumoylation sites. Functional sumoylation on non-consensus sites, however, has been widely observed. For example, the yeast core histones contain multiple mass-spectrometry validated non-consensus sites that can not be identified by any of the existing SUMO prediction programs , . Furthermore, 26% of experimentally validated SUMO conjugation sites are non-consensus . We found that deletion of the C-terminal 25 amino acids of Ras1 or mutations of all lysines in this region abolished Ras sumoylation (Figure 3G), whereas the C-terminal Ras1165–189 peptide was sumoylated to yield a pattern of bands in SDS-PAGE similar to that observed for wild type Ras1 (Figure 3G), indicating that the hypervariable C-terminal region of Ras1 contains the sites of SUMO modification.
SUMO is required for the syncytial mitotic cycles
The SUMO pathway has been shown to be required for cell cycle progression in other organisms . Consistent with this, our proteomic analysis found proteins involved in cell cycle regulation to be significantly over-represented among SUMO conjugates in the early Drosophila embryo (Table 2, Table S6), and moreover our findings significantly expands the list of know sumoylated cell cycle regulators. To determine if the lethality caused by a reduced maternal supply of SUMO is due to cycling defects, 0- to 3-hour wild-type and sumo04493 GLC embryos were stained with DAPI to visualize DNA. During the initial stage of Drosophila embryogenesis, 13 nuclear cleavage cycles occur rapidly and synchronously in a syncytium (Figure 4A). We observed that over 50% of the sumo04493 GLC embryos exhibited a broad spectrum of nuclear cycle defects, including irregular size and distribution of nuclei, asynchronous nuclear division, abnormal interphase chromosome structure, overly condensed chromosomes, loss of sister chromatid cohesion during metaphase, polyploidy, chromosome clustering, fragmentation, and chromosome bridges (Figure 4B–E). Multiple nuclear division defects were often observed in a single embryo (Figure 4B). We also observed similar, although somewhat less penetrant, nuclear cleavage cycle defects in embryos resulting from GLC of a ubc9 (the SUMO conjugating enzyme) hypomorphic allele , semi118 (Figure 4F). The diverse cycling defects observed in the sumo and ubc9 GLC embryos indicate broad involvement of SUMO in multiple stages of the nuclear cycle, and are consistent with our proteomic analysis showing a significant enrichment in SUMO targets with cell cycle functions.
A) A wild type syncytial blastoderm embryo in metaphase. B–E) Representative nuclear cycle defects in DAPI stained sumo04493 GLC embryos. DAPI staining revealed multiple cell cycle defects in sumo04493 GLC embryos. Panel B shows a sumo04493 mutant embryo, while B′ and B″ are magnified views of two regions of the embryo in B. The arrow in B′ points to an abnormally large cluster of chromosomes, indicating polyploidy, and the arrow in B″ points to a prominent chromosome bridge. C) Abnormal chromosomal organization. The arrow in D highlights a possible cohesion defect. The left arrow in E points to a cluster of hypercondensed chromosomes, and the right arrow points to chromosome fragments. F) Frequency of cell cycle defects in sumo and ubc9 mutant GLC embryos.
A–B) Syncytial blastoderm Ore-R embryos. C) Uncoupled chromosome and centrosome replication observed in sumo04493 GLC embryos. D–J) In sumo04493 GLC embryos, multiple defects in metaphase spindle morphology are observed, including examples of anastral (arrow in F), monoastral (H), multipolar (E, G, arrows), as well as unfocused mitotic spindles (D′ and arrows in I, J). The embryos were stained for DAPI (blue), centrosomin (CNN, green), and α-Tubulin (red).
The mitotic cycle defects in sumo mutant GLC embryos were further characterized through visualization of centrosomes and microtubules. Correct spatial organization and synchronous nuclear division of the early embryos requires a high degree of coordination between centrosome duplication, microtubule dynamics, and changes in nuclear structure. Abnormalities, such as asynchronous division, irregular nuclear spacing, and polyploidy, observed in sumo04493 GLC embryos, suggest an uncoupling of these events. In wild type syncytial blastoderm embryos, each set of chromosomes is associated with a pair of centrosomes (Figure 5A, B). sumo04493 embryos often contain a reduced number of nuclei in relation to the centrosome pairs (Figure 5C), a common mitotic defect in Drosophila –.
Defects in mitotic spindle organization and attachment to centrosomes and chromosomes were also observed in sumo GLC embryos (Figure 5D–J). Monoastral, anastral, and multipolar spindles, as well as unfocused broad-based spindles were documented. These results suggest that sumoylation is important in coordinating multiple events of mitosis, such as centrosome replication, centrosome-spindle association, and spindle-chromosome attachment. The broad spectrum of mitotic defects seen in the sumo GLC embryos is consistent with the defective mitotic spindle assembly induced by SUMO RNAi in Drosophila S2 cells previously observed , as well as with our fly SUMO-ome, which also suggests a broad role for SUMO in mitosis.
SUMO is required for cell cycle progression in S2 cells and larval tissue
The syncytial nuclear cleavage cycles are non-canonical mitotic cycles lacking the gap phases as well as cytokinesis. To determine if sumoylation is essential for cells undergoing a canonical G1-S-G2-M cell cycle, we investigated the requirement for SUMO in cell cycle progression of cultured cells and larval wing imaginal discs. The DNA content of S2 cells was measured by flow cytometry to assess cell cycle stage following three to five days of SUMO RNAi. The fraction of G2/M phase cells gradually diminished, suggesting G1/S arrest as a result of sustained low levels of SUMO (Figure 6A, B).
A) FACS analysis of DNA content in S2 cells treated with dsRNA against SUMO or YFP for 3, 4, or 5 days. The overall distribution (unfilled curve) has been fit to show the G1 (2N) and G2/M (4N) cells (red-filled curves), the S phase cells (hatched curve), cells with less than 2N DNA content (blue-filled curves), and cells with more than 4N DNA content (green-filled curves). B) The percentages of YFP or SUMO dsRNA treated cells in G1, S, and G2/M phases. C–G) SUMO is required for mitosis in wing imaginal discs. C) This MS1096>laminGFP wing disc shows the domain of MS1096-Gal4 expression. Mitotic cells were marked by pHH3 staining in MS1096 (D), or MS1096>sumoRNAi (E) third instar larval wing discs (all discs are oriented with dorsal on the top and anterior on the right). C′, D′, and E′ show DAPI overlay of the images on the left. F) The numbers of pHH3 positive cells in multiple discs were counted and averaged (standard errors are indicated). G) Adult wings from the MS1096 (left) or MS1096>sumoRNAi (right) flies.
Imaginal discs undergo rapid proliferation during larval development. We knocked down SUMO in larval tissue by RNAi using Gal4 to drive the expression of a sumo hairpin RNA. The effectiveness of the RNAi was demonstrated by anti-SUMO staining of discs containing clones of cells that express sumo hairpin RNA (Figure S5). To examine the effects of SUMO knockdown on the wing imaginal disc cell cycle, we employed the MS1096-Gal4 driver, which directs a high level of expression in the dorsal compartment and a lower level of expression in the ventral compartment of the third instar wing pouch (Figure 6C, C′). The wing discs were stained with an antibody against phosphohistone H3 (pHH3) to mark the mitotic cells (Figure 6D–E′). sumo RNAi resulted in a marked reduction of pHH3 positive cells throughout the wing pouch (Figure 6E, E′, F). The proliferation defect was especially pronounced in the dorsal compartment where the Gal4 driver is most active (Figure 6E, E′), and was also manifested in adult wings, which were greatly reduced in size and misshapen (Figure 6G). A similar requirement for SUMO in cell proliferation was also observed in eye discs when ey-Gal4 was used to drive the expression of sumo hairpin RNA (data not shown). Thus, SUMO is required for both the atypical mitotic cycle that occurs in nuclear cleavage stage embryos as well as the canonical cell cycles that occur in cultured cells and larval imaginal discs.
Dynamic localization of SUMO during mitosis
Our immunofluorescence studies on sumo GLC embryos clearly indicate that SUMO plays diverse roles at various stages of the mitotic cycle. To gain further insight into the role of SUMO in the cell cycle, we systematically documented the localization of SUMO through stages of the nuclear cleavage cycle (Figures 7A, C). Previous studies have shown that SUMO assumes a predominantly nuclear distribution in the early embryo at interphase . This observation was confirmed in this study. During interphase and prophase, SUMO is distributed throughout the nucleus, but is concentrated in puncta of unknown structure (Figure 7A). As the embryonic nuclei progress to metaphase, SUMO associates with the condensing chromosomes and appears to be concentrated in regions around the centromeres, as marked by the points of closest association between the spindle and the chromosomes (Figure 7C, left panels). This pericentromeric localization is also consistent with the central ring of anti-SUMO staining observed in the polar body chromosomes (Figure 7B), and with previous observations of SUMO association with the chromocenter of the polytene chromosome , .
A) Interphase nuclei of syncytial blastoderm stage Oregon-R embryo stained with SUMO antibodies (green) and DAPI (blue). B) Polar body stained with SUMO antibodies (green) and DAPI (blue) showing SUMO localization to the pericentromeric regions of the polar body chromosomes. C) Metaphase, anaphase, and telophase nuclei of syncytial blastoderm embryo stained with SUMO antibodies (green), α-tubulin antibodies (red), and DAPI (blue). Top panels show the SUMO/DAPI merge, while bottom panels show the SUMO/α-tubulin merge. White arrowheads in left panels point to pericentromeric SUMO in one of the nuclei. Gray arrows in the center and right panels point to spindle midzone SUMO. D) HA-Ubc9-expressing S2 cells  arrested at prometaphase with 25 µM colchicine and stained with SUMO antibodies (red), HA antibodies (green, revealing localization of HA-Ubc9), and DAPI (blue). Ubc9 is diffusely localized but also exhibits intense puncta that colocalize with SUMO near the kinetochores. E) S2 cell arrested at prometaphase with colchicine and stained with DAPI (blue), and antibodies against SUMO (red) and Polo (green). SUMO and Polo partially colocalize at the outer kinetochore regions (white arrows point to the kinetochores of one pair of sister chromatids). F) Syncyticial embryo expressing Polo-GFP (green; Polo-GFP flies were obtained from Dr. Claudio Sunkel) was stained with DAPI (blue) and antibodies against SUMO (red). Polo and SUMO colocalize to the midbody (indicated for one nucleus by the gray arrow).
The association of SUMO with the pericentromeric regions of the chromosomes persists during anaphase and telophase (Figure 7C, center and right panels). In addition, SUMO also localizes to the spindle midbodies, which are clearly framed by the midzone spindles (the spindles between the segregating sister chromatids), as they form during anaphase (Figure 7C, center panels). Localization of SUMO to the midbody is even more apparent during telophase (Figure 7C, right panels). The association of SUMO with the chromosomes and midbody was also observed in S2 cells (Figure 7E and data not shown). The association of SUMO with the chromosomes throughout mitosis and its localization to the midbodies during anaphase and telophase is consistent with our observation that SUMO plays critical roles during multiple stages of the mitotic cycle. These findings are also consistent with a report that SUMO-2/3 in HeLa cells associates with the mitotic chromosome, while SUMO-1 associates with the spindle midzone . Apparently, the multiple mitotic functions of SUMO carried out by the single SUMO isoform in Drosophila have been divided among multiple SUMO isoforms in vertebrates.
The localization of SUMO during mitosis can be observed in more detail in cultured cells. Cells arrested at prometaphase by colchicine treatment display SUMO localization on condensed chromosomes at the outer kinetochore and inner centromeric regions (ICR) (Figure 7E). Like SUMO, Ubc9 assumes a predominantly nuclear distribution during interphase (data not shown). During mitosis, Ubc9 staining exhibited a largely diffuse distribution along with concentrated foci that overlapped the domain of SUMO localization at the centromeric regions and kinetochores, suggesting that active sumoylation is taking place at those locations (Figure 7D).
Polo, one of the SUMO targets identified in our proteomic screen and validated using the bacterial sumoylation system (Table 2, Figure S4C), is the only Drosophila Polo-like kinase family protein. Polo is involved in multiple stages of cell cycle regulation, localizes to the outer kinetochore early in mitosis, and subsequently relocalizes to the midbodies late in mitosis . Given these similarities between the functions of SUMO and Polo, we decided to compare directly the localization of SUMO and Polo during the mitotic cycle. During interphase, Polo and SUMO occupy distinct cellular compartments, being cytoplasmic and nuclear, respectively (data not shown). During prometaphase and metaphase, partial overlap between SUMO and Polo is observed at the outer kinetochore (Figure 7E, and data not shown). At later stages of mitosis (anaphase and later), Polo is localized to the midbody, and again exhibits incomplete overlap with SUMO (Figure 7F). This partial co-localization during multiple phases of mitosis suggests that Polo could be one of the SUMO substrates at the kinetochores. However, it also indicates that there are likely additional SUMO substrates at the kinetochores and inner centromeric region, and that not all kinetochore-associated Polo is sumoylated.
The SUMO conjugation pathway is highly conserved in eukaryotic evolution, and plays many key regulatory roles. Drosophila embryos contain high levels of maternally supplied SUMO, indicating that sumoylation may be especially important in early Drosophila embryogenesis. Accordingly, reduced maternal expression of SUMO has pleiotropic effects in oogenesis and embryogenesis. Our proteomic, genetic, and cell culture analyses converge to support roles for protein sumoylation in Ras signaling, mitotic progression, and embryonic pattern formation.
SUMO and Ras signaling
The Ras signaling cascade is activated by a variety of RTKs including EGFR, and controls cell proliferation and differentiation as well as a large number of developmental patterning processes, such as patterning of the eggshell –. Activation of EGFR in the dorsal follicle cells during oogenesis leads to the sequential activation of Ras, Raf, MEK, and MAPK, and results in the upregulation of RTK target genes . Complex positive and inhibitory feedback loops ultimately result in the specification of the dorsal follicle cells, which later secrete the dorsal eggshell, including the dorsal appendages , .
Previous genetic screens for mutations that enhance the eggshell ventralization phenotype of a weak hypomorphic Ras1 allele suggested a role for SUMO in the Ras pathway downstream of EGFR activation . In our analysis of the recessive sumo mutant phenotype, we observed fused or single dorsal appendages, indicative of eggshell ventralization and consistent with the attenuation of EGFR signaling. Since the eggs under study resulted from sumo GLCs, the observed eggshell defect could reflect a function for SUMO upstream of EGFR in the production or secretion by the germ line of EGFR ligands. However, since sumo mutant clones are also present in the follicle cells of the GLC egg chambers, the eggshell ventralization phenotype we observe is also consistent with a role for SUMO downstream of EGFR activation in the follicle cells. Interestingly, sumoylation pathway proteins in C. elegans were also shown to interact with the Ras signaling pathway . Our cell culture experiments support a role for protein sumoylation in Ras signaling that is downstream of EGFR and upstream of, or parallel to, Ras activation. SUMO may directly modulate Ras1 function since Ras1 was found in our proteomic analysis and confirmed as a sumoylation substrate in our bacterial sumoylation assay.
SUMO and cell cycle progression
Sumoylation is implicated in cell cycle regulation in many organisms . In this study, we observed diverse nuclear cleavage defects in sumo GLC embryos suggestive of multiple roles for SUMO in coordinating the chromosome cycle. The phenotypes, including chromosome hypercondensation, aberrant segregation, and polyploidy, are reminiscent of the defects observed in Ubc9-deficient mouse embryos and Drosophila embryos mutant for pias, a possible SUMO ligase , , indicating conservation of SUMO cell cycle functions in metazoan evolution. We also demonstrated a requirement for SUMO in cell cycle progression in cultured cells and in larval imaginal discs by RNAi-mediated SUMO knockdown. While the cell proliferation defect in SUMO mutant wing discs could result from a requirement for SUMO for the function of many of the same cell cycle proteins found in our proteomic screen of early embryos, it could also reflect a role for SUMO in the function of Vg, a previously identified wing disc sumoylation target  known to be required for wing growth –.
In agreement with the diverse cell cycle defects in sumo mutant embryos and other tissues, a spectrum of cell cycle regulators involved in multiple stages of the cell cycle were identified in our SUMO proteomic screens (Table 2). For example, the failure of cultured cells to progress to G2/M could reflect a role for SUMO in DNA replication, which is consistent with our finding that PCNA, RFC2, Topoisomerase I, and Topoisomerase II are all targets of sumoylation (Table 2 and Figure 1C). A role for SUMO in the function of Polo kinase could further explain some of the observed cell cycle defects since Polo has multiple roles in the cell cycle , . Other sumoylation targets identified in our screen, including PP2A, Arp3, Cofilin (Twinstar), Mago Nashi, and Profilin, are also consistent with multiple roles of SUMO in mitosis.
The requirement for SUMO throughout mitosis is further supported by its dynamic, mitotic stage-dependent, localization. At prometaphase and metaphase, sumoylated proteins are concentrated at the kinetochores and ICR, partially co-localizing with Polo. Ubc9 co-localized with SUMO at the kinetochore-centromeric regions during mitosis, suggesting that active sumoylation is taking place at those locations. It is likely that many kinetochore and centromere localized proteins are targeted by SUMO, and cycles of sumoylation and de-sumoylation may help to propel unidirectional mitotic progression.
While a number of studies have connected sumoylation to centromere and kinetochore functions , spindle midbody localization of SUMO has not been widely reported , . The midbody is a structure derived from the spindle midzone that contains proteins indispensable for cytokinesis . SUMO association with the midbody, which we have observed in both syncytial embryos and cultured cells beginning with anaphase and extending through cytokinesis, therefore argues for a role of sumoylation in the completion of cell division. The midbody proteome has been dissected recently in mammalian cells, revealing a large collection of proteins, including membrane associated proteins, microtubule associated proteins, and kinases . Homologs of a number of these proteins, such as Arp3, Cofilin (Twinstar), Mago Nashi, Polo, PP2A, and Profilin, were all identified in our Drosophila SUMO proteomic screens (Table 2), reinforcing the notion that SUMO is involved in midbody function.
Cytokinesis does not occur in nuclear cleavage stage embryos. However, the midbody has an important role in maintaining the separation of telophase sister nuclei , a process that could be related to the formation of pseudocleavage furrows at the end of each nuclear cleavage cycle. Disruption of midbody function in SUMO deficient embryos may therefore account for some of the mitotic defects we observe in the syncytial embryo, including polyploidy.
SUMO and embryonic patterning
We observe diverse patterning defects among the sumo GLC embryos that developed a cuticle. In accordance with this observation, three absolutely critical patterning proteins, Dorsal, Bicoid, and Hunchback, are among the sumoylated proteins we detected in early embryo extracts (Table 2). Previous studies have shown that sumoylation of Dorsal potentiates its activity during the immune response perhaps by making it a more potent transcriptional activator . While an earlier study showed that the loss of Ubc9 results in a hunchback-like anterior patterning phenotype and defective nuclear transport of Bicoid , our study is the first to show that Hunchback, and its activator Bicoid, are direct SUMO conjugation targets. Thus, it is possible that sumoylation of these transcription factors plays a direct role in anterior patterning.
Posterior patterning and germ line specification depend upon the posterior localization of the oskar transcript. We identified several oskar mRNP components, including Mago Nashi, Tsunagi, Cup, Hrb27C, and Smaug, as sumoylation targets (Table 2 and 3), which have essential roles in the regulation of oskar mRNA localization and translation . This interesting and novel finding suggests a role of SUMO in regulating the functions of maternal mRNA by modifying components of oskar mRNP, and therefore could explain some of the pleiotropic defects observed in the embryonic patterning of embryos resulting from sumo mutant GLCs.
The oskar mRNP is one of several instances in which multiple members of the same complex appear to be direct targets of sumoylation. For example, our screen turned up several members of the multi-aminoacyl-tRNA synthetase complex, as well as multiple ribosomal proteins (Table 3). Screens for sumoylation targets in S. cerevisiae have similarly detected multiple sumoylation targets in the same complex , . This suggests that oligomeric protein complexes can be targeted as a whole for sumoylation and/or that sumoylation may have a general role in stabilizing protein complexes.
In contrast to previous studies in yeast and mammalian cell culture , , , –, relatively few transcription factors were identified in our study. This difference in fact accurately reflects the unique metabolic state of the pre-cellularization embryo. During the first two hours of Drosophila embryonic development, rapid nuclear divisions depend upon a complex dowry of maternally supplied proteins, as transcription of the zygotic genome has not yet begun. Instead, the proper localization and accurately regulated translation of maternally supplied mRNAs is essential for establishing the system of positional information that will later direct the spatially regulated transcription of the zygotic genome . Thus, the relatively small and selective group of sumoylated transcription factors, along with the large number of factors that control mRNA translation and localization found in our screen, is consistent with regulatory roles for SUMO in this critically important stage of fly development.
In conclusion, our genetic, cellular, and proteomic studies of sumoylation suggest mechanisms for known biological roles of the SUMO pathway and also uncover novel connections between sumoylation, signal transduction, the cell cycle, and development. Furthermore, our SUMO conjugated proteome should serve as a rich resource for those studying the roles of sumoylation in metazoan development.
Materials and Methods
Sequences encoding the (His)6- and FLAG-tags (HHHHHHDYKDDDDK) were added to the 5′ end of the sumo coding region by PCR, using primers containing the sequences corresponding to those tags as well as NotI and XbaI restriction sites (primer sequences are given in supporting document S1). The resulting PCR product was digested and ligated into the pUASp vector  to produce pUASp-H6Flag-SUMO.
To construct the plasmid termed “Quartet” or “Q”, which expresses the Drosophila SUMO pathway in a single vector, components of the pathway were first cloned into two Duet vectors (Novagen), then combined into a single vector. Briefly, the mature form of SUMO, SUMOGG (last 2 amino acids omitted), and ubc9 were amplified from cDNAs by PCR, and cloned into pRSF-Duet-1 MCS1 at the EcoRI/NotI sites, and MCS2 at the NdeI/XhoI sites, respectively, to generate pRSF-SUMOGG-Ubc9. Similarly, sae2 and sae1 were cloned into the MCS1 and MCS2 of the pCDF-Duet-1 to create pCDF-SAE2-SAE1. Subsequently, the pCDF-SAE2-SAE1 was digested with PfoI, filled in with Klenow, and then cut at the AgeI site, and the sae2-sae1 fragment was then introduced by ligation into the pRSF-SUMO-Ubc9 vector, which has been digested with Bsu36I, blunted using Klenow, and then digested with AgeI, to obtain, QSUMO (pRSF-SUMOGG-Ubc9-SAE2-SAE1). The control vector, QΔGG (pRSF-SUMOΔGG-Ubc9-SAE2-SAE1), which expresses a conjugation defective form of SUMO, SUMOΔGG, in place of SUMOGG, was constructed using the same strategy. The ORFs of sumo and sae2 were cloned in frame with an N-terminal (His)6 tag, and ubc9 and sae1 were cloned in frame with a C-terminal S-tag.
The pGEX-loxP plasmid was generated by inserting a sequence containing the loxP recombination site and bacterial promoter, for Cre recombination and an antibiotic selection gene, respectively (sequence information is available in The Creator Cloning System Manual, Clontech), into pGEX-4T-1 (Amersham) at the EcoRI and XhoI sites. This vector serves as an acceptor vector for generating an in-frame amino terminal GST fusion with open reading frames (ORFs) that have been introduced into a donor vector. The donor pDNR-Dual vectors for PCNA (BS06345), RfC2 (BS06321), CtBP (BS10020), HP1 (BS03857), and Ras1 (BS04665) were purchased from the Drosophila Genomics Resource Center (DGRC). The ORFs of 14-3-3ζ, squid, polo, and eIF4E were amplified from cDNA clones (RH61958, LD29474, BO04660, and RE36735 from the DGRC) by PCR, and introduced into pDNR-Dual (Clontech) at the HindIII and SalI sites utilizing the In-Fusion PCR Cloning Kit (Clontech). The ORFs were then transferred from the pDNR vectors into the acceptor, pGEX-loxP vector, by Cre recombination (Clontech). The eIF4E and Ras1 ORFs were also recombined into the S2 cell expression acceptor vector, pMK33FlagHis-BD (obtained from Dr. Mark Stapleton).
The RasK104R point mutation was generated by PCR-based site-directed mutagenesis of pDNR-Dual-Ras1. The Ras1–164, Ras7KR, and Ras165–189 were cloned into pDNR-Dual vector by insertion of PCR products (see supporting document S1 for primer sequences). The sequences encoding these Ras variants were then recombined into the pGEX-loxP vector. The RasV12 point mutation was created by PCR-based mutagenesis of the pMK33FlagHis-Ras1 vector. All plasmids generated in this study were sequenced to verify the presence of the correct inserts and sequences (UCLA Genotyping and Sequencing Core).
Two-step purification of SUMO conjugates
Wild type Oregon-R embryos or embryos expressing tagged SUMO, were collected at 25°C over a three-hour period, washed, incubated at 37°C for an additional 45 minutes, then immediately frozen under liquid nitrogen. Five grams of frozen embryos were ground to power under liquid nitrogen and suspended in 20 ml of Urea Binding buffer A (100 mM NaH2PO4, 10 mM Tris, pH 8.0, 8 M urea, 5 mM imidazole, and 1% CHAPS), which was freshly supplemented with 40 mM N-ethylmaleimide (NEM, Sigma) and one Mini Complete Protease Inhibitor Cocktail Tablet (Roche). The suspension was further lysed with a French Press, and the lysate was centrifuged at 32,000×g at 4°C for 20 min. The supernatant was filtered using Miracloth (CBC) to remove lipid clumps, and then mixed with 1 ml of buffer A equilibrated Ni-NTA beads (QIAGEN) at 22°C for one hour and 30 min in an Econo-column (BioRad). After removal of the unbound material by gravity flow, the Ni-NTA beads were washed twice with total of 40 ml of Wash buffer B (100 mM NaH2PO4, 10 mM Tris, pH 8.0, 8 M urea, 5 mM imidazole, and 1 mM PMSF), and eluted 4 times with 1 ml of Elution buffer C (100 mM NaH2PO4, 10 mM Tris, pH 8.0, 8 M urea, 20 mM EDTA, and 400 mM imidazole).
The eluted proteins were immediately dialyzed against three liters of TBS (50 mM Tris, pH 7.4, 300 mM NaCl, 1 mM EDTA, and 5% glycerol) over 2 hours at 4°C. The dialyzed sample was further diluted five-fold with cold TBST buffer (TBS plus 1% Triton X-100, and supplemented with 20 mM NEM and protease inhibitor cocktail), and incubated with 100 µl of anti-FLAG agarose (Sigma) at 4°C overnight. The next day, the beads were separated from unbound proteins by centrifugation, washed four times with TBST, and transferred to a spin collection column (Zymo) to remove TBST. The proteins were eluted from the beads with NuPAGE LDS loading buffer (Invitrogen) at 70°C for 15 min, and a portion of the eluted proteins were later separated by SDS-PAGE and analyzed by Western blot or in-gel trypsin digest followed by LC-MS/MS analysis of the tryptic peptides.
Protein identification by LC-MS/MS
The sliced SDS-PAGE gels were digested with sequencing-grade trypsin (Promega). The digested peptides were extracted from the gel slices using 50% acetonitrile/0.1% trifluoroacetic acid (TFA) in water. The extracts were dried down, resuspended in 0.1% formic acid/water, and LC-MS/MS of the peptide mixtures was performed on an Applied Biosystems QSTAR XL (ESI-QqTOF) mass spectrometer coupled with an LC Packings nanoflow HPLC system, through a nanoelectrospray ionization source (Protana). A homemade trap column (150 µm×2 mm) and nano-column (75 µm×150 mm) packed with Jupiter Proteo C12 resin (particle size 4 µm, Phenomenex) were employed for the nano-flow HPLC peptide separation using an 80-minute gradient. Product ion (MS/MS) spectra of the peptides separated by HPLC were recorded and then submitted to the Mascot database search engine (Matrix Science) for protein identification. Positive protein identification was based on standard Mascot scoring criteria (p<0.05) for statistical analysis of the LC-MS/MS data. The peptide assignments in the Mascot database search results were then manually inspected for validation.
The bacterial sumoylation assay
A vector encoding a candidate SUMO conjugation target fused to GST was co-transformed into BL21 cells (Novagen) with either the QSUMO or the control QΔGG expression vectors. Several transformed bacterial colonies, selected for kanamycin and ampicillin resistance, were picked to inoculate 0.5 ml cultures of non-inducing medium, and shaken at 37°C overnight. This culture was then used to inoculate 0.5 ml culture in auto-inducing medium overnight at 25°C to induce expression of all five proteins. The auto-induction was carried out as described by F. William Studier . These small-scale cultures were compared for levels of expression of the GST fusion protein by anti-GST Western blot. The culture with the highest expression was then scaled up to a 50 mL culture. The bacteria grown to saturation (O.D.600 of 12–18) were collected by centrifugation, and the GST-fusion protein was purified with glutathione beads according to the manufacturer's protocol (Amersham). The eluted proteins were subsequently resolved by SDS-PAGE, and analyzed by Sypro Ruby protein staining and immunoblotting.
Fly stains and crosses
Flies were maintained on standard medium at 25°C. The pUASp-H6Flag-SUMO vector was introduced into w1118 flies by embryo injection (Model System Genomics of Duke University). Multiple lines with insertions into the X, second, or third chromosomes were recovered. The MatGal4 driver, which encodes Gal4-VP16 under the control of a maternally active α4-Tubulin promoter was generously provided by Dr. Daniel St. Johnston.
The sumo mutant fly stocks used in this study are P[ry[+t7.2] = PZ], smt304493/CyO; ry506 (referred to as sumo04493 in this study; obtained from Bloomington Stock Center) and Df1w67c23, y1; P[lacW]smt3k01211/CyO (referred to as sumok01211; obtained from Dr. Jon Schnorr). The original CyO balancer of the sumo mutant lines was replaced with a GFP expressing CyO balancer (CyO, ActGFP) to allow homozygous SUMO mutant flies (which lack GFP) to be distinguished from their heterozygous siblings. The fly strain containing a second chromosome insertion that expresses a hairpin RNA against the SUMO gene under UAS control (UAS-sumoRNAi) was obtained from the Vienna Drosophila RNAi Center. Since the UAS-sumoRNAi flies are homozygous sterile, we balanced the line with CyO, ActGFP to allow the UAS-sumoRNAi carrying flies to be distinguished.
An FRT site (FRT40A) was introduced by recombination onto the chromosome arms carrying the mutant sumo alleles. The FRT40A line of mutant ubc9, semi118, was a obtained from Dr. Sochi Tanda . The standard dominant female sterile FLP/FRT protocols were followed to generate germ line clones of sumo P-element alleles or semi118 .
Scanning electron microscopy and cuticle preparation of embryos
Fly embryos were attached to metal mounts as uncoated samples using fingernail polish. Images were digitally acquired using a Hitachi S-2460N Scanning Electron Microscope at a ‘high pressure’ setting of 30 Pa using a Robinson detector. To prepare embryo cuticles, dechorionated and devitellinized embryos were mounted on slides in Hoyer's mounting medium , and imaged with dark field optics on a Zeiss Axioskop microscope.
Immunofluorescence and Western blotting
Primary antibodies used for immunofluorescence were rabbit anti-SUMO , mouse anti-α-tubulin (Sigma), rabbit anti-CNN , rabbit anti-pHH3 (pSer10, Sigma), mouse anti-Polo (a gift of Dr. Claudio Sunkel), and mouse anti-HA (Sigma). Secondary antibodies used were goat anti-rabbit or goat anti-mouse antibodies conjugated with Alexa Fluor 488 or Alexa Fluor 568 (Molecular Probes). DNA was stained with 1 µg/ml 4′,6-diamidino-2-phenylindole (DAPI). Confocal images of the embryos and imaginal discs were obtained on a Leica one-photon confocal laser scanning microscope (Leica Microsystems, Heidelberg). The S2 cells were visualized on a Deltavision Spectris deconvolution microscopy system (Applied Precision), and the images were deconvolved using Applied Precision software.
Antibodies used for immunoblotting were rabbit anti-ERK (Sigma), mouse anti-dpERK (Sigma), rabbit anti-MEK (Cell Signaling), rabbit anti-pMEK (Cell Signaling), mouse anti-FLAG (Sigma), rabbit anti-SUMO , rabbit anti-GST (Abcam), and mouse anti-poly-His (Sigma). Signal detection was achieved with secondary antibody conjugated with horseradish peroxidase (HRP) (CalBiochem) and SuperSignal West Pico substrates (Pierce).
Cell culture, RNA interference, and cell cycle analysis
Drosophila cultured cells were maintained at 24°C in Schneider's insect medium (Gibco) supplemented with 10% fetal bovine serum (JHR) and antibiotics (Invitrogen). PCR products with T7 promoters on both ends (primer information is given in supporting document S1) were used as templates for in vitro transcription to make dsRNAs using the Megascript RNAi kit (Ambion). The dsRNA was introduced into the cultured cells as described . To establish stable cell lines expressing FLAG-(His)6-RasV12 or FLAG-(His)6-eIF4E under inducible condition, the pMK33FlagHis-RasV12 or pMK33FlagHis-eIF4E plasmid was transfected into S2 cells using Effectene (QIAGEN). The transfected cells were then selected with hygromycin until stable cell lines were established. For the flow cytometry, cells were suspended in Propidium Iodide DNA staining buffer , and analyzed on a Becton Dickinson FACScan Analytic Flow Cytometer at the UCLA Flow Cytometry Core Facility.
SUMO conjugates identified in two-step purification under denaturing conditions
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Proteins identified in one-step purification under native conditions
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Comparison of proteins found in one-step purification from heat shocked and non-heat shocked embryos
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GO categories of SUMO conjugates identified in two-step purification - biological processes ontology
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GO categories of SUMO conjugates identified in two-step purification - cellular component ontology
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Comparison on the levels of enrichment in Biological Processes of the 2h AEL embryos proteome to that of the SUMO conjugates identified in two-step purification
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Supplementary methods and figure legends
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Detection of native and tagged SUMO in an anti-SUMO immunoblot
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FLAG-IP of embryos expressing tagged-SUMO or control embryos
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sumo P-element mutant expresses reduced levels of sumo mRNA
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Bacterial sumoylation assays on HP1, squid, and Polo
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We would like to thank Dr. Rachel Loo, Jimmy Ytterberg, and Pinmanee “Charm” Boontheung for their help and advice with the MS/MS analysis, and Tad Kawashima for technical assistance with cloning and protein expression. We are very grateful to Drs. Jon Schnorr, Adam Friedman, Ben-Zion Shilo, Thomas Kaufman, Mark Stapleton, Claudio Sunkel, Marc Therrien, Shanti Chandrashekaran, Lawrence Marsh, Sochi Tanda, and Daniel St. Johnston, who provided reagents used in this research. We would also like to acknowledge the Bloomington and Vienna fly stock centers for fly lines. Confocal images were obtained in the UCLA CNSI Advanced Light Microscopy/Spectroscopy Shared Facility.
Conceived and designed the experiments: MN AJC. Performed the experiments: MN YX. Analyzed the data: MN YX JL AJC. Contributed reagents/materials/analysis tools: MN YX JL AJC. Wrote the paper: MN AJC.
- 1. Kerscher O, Felberbaum R, Hochstrasser M (2006) Modification of proteins by ubiquitin and ubiquitin-like proteins. Annu Rev Cell Dev Biol 22: 159–180.O. KerscherR. FelberbaumM. Hochstrasser2006Modification of proteins by ubiquitin and ubiquitin-like proteins.Annu Rev Cell Dev Biol22159180
- 2. Geiss-Friedlander R, Melchior F (2007) Concepts in sumoylation: a decade on. Nat Rev Mol Cell Biol 8: 947–956.R. Geiss-FriedlanderF. Melchior2007Concepts in sumoylation: a decade on.Nat Rev Mol Cell Biol8947956
- 3. Zhao J (2007) Sumoylation regulates diverse biological processes. Cell Mol Life Sci 64: 3017–3033.J. Zhao2007Sumoylation regulates diverse biological processes.Cell Mol Life Sci6430173033
- 4. Martin S, Wilkinson KA, Nishimune A, Henley JM (2007) Emerging extranuclear roles of protein SUMOylation in neuronal function and dysfunction. Nat Rev Neurosci 8: 948–959.S. MartinKA WilkinsonA. NishimuneJM Henley2007Emerging extranuclear roles of protein SUMOylation in neuronal function and dysfunction.Nat Rev Neurosci8948959
- 5. Dohmen RJ, Stappen R, McGrath JP, Forrova H, Kolarov J, et al. (1995) An essential yeast gene encoding a homolog of ubiquitin-activating enzyme. J Biol Chem 270: 18099–18109.RJ DohmenR. StappenJP McGrathH. ForrovaJ. Kolarov1995An essential yeast gene encoding a homolog of ubiquitin-activating enzyme.J Biol Chem2701809918109
- 6. Johnson ES, Schwienhorst I, Dohmen RJ, Blobel G (1997) The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer. Embo J 16: 5509–5519.ES JohnsonI. SchwienhorstRJ DohmenG. Blobel1997The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer.Embo J1655095519
- 7. Seufert W, Futcher B, Jentsch S (1995) Role of a ubiquitin-conjugating enzyme in degradation of S- and M-phase cyclins. Nature 373: 78–81.W. SeufertB. FutcherS. Jentsch1995Role of a ubiquitin-conjugating enzyme in degradation of S- and M-phase cyclins.Nature3737881
- 8. al-Khodairy F, Enoch T, Hagan IM, Carr AM (1995) The Schizosaccharomyces pombe hus5 gene encodes a ubiquitin conjugating enzyme required for normal mitosis. J Cell Sci 108(Pt 2): 475–486.F. al-KhodairyT. EnochIM HaganAM Carr1995The Schizosaccharomyces pombe hus5 gene encodes a ubiquitin conjugating enzyme required for normal mitosis.J Cell Sci108(Pt 2)475486
- 9. Shayeghi M, Doe CL, Tavassoli M, Watts FZ (1997) Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance. Nucleic Acids Res 25: 1162–1169.M. ShayeghiCL DoeM. TavassoliFZ Watts1997Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance.Nucleic Acids Res2511621169
- 10. Tanaka K, Nishide J, Okazaki K, Kato H, Niwa O, et al. (1999) Characterization of a fission yeast SUMO-1 homologue, pmt3p, required for multiple nuclear events, including the control of telomere length and chromosome segregation. Mol Cell Biol 19: 8660–8672.K. TanakaJ. NishideK. OkazakiH. KatoO. Niwa1999Characterization of a fission yeast SUMO-1 homologue, pmt3p, required for multiple nuclear events, including the control of telomere length and chromosome segregation.Mol Cell Biol1986608672
- 11. Jones D, Crowe E, Stevens TA, Candido EP (2002) Functional and phylogenetic analysis of the ubiquitylation system in Caenorhabditis elegans: ubiquitin-conjugating enzymes, ubiquitin-activating enzymes, and ubiquitin-like proteins. Genome Biol 3: RESEARCH0002.D. JonesE. CroweTA StevensEP Candido2002Functional and phylogenetic analysis of the ubiquitylation system in Caenorhabditis elegans: ubiquitin-conjugating enzymes, ubiquitin-activating enzymes, and ubiquitin-like proteins.Genome Biol3RESEARCH0002
- 12. Epps JL, Tanda S (1998) The Drosophila semushi mutation blocks nuclear import of bicoid during embryogenesis. Curr Biol 8: 1277–1280.JL EppsS. Tanda1998The Drosophila semushi mutation blocks nuclear import of bicoid during embryogenesis.Curr Biol812771280
- 13. Nacerddine K, Lehembre F, Bhaumik M, Artus J, Cohen-Tannoudji M, et al. (2005) The SUMO pathway is essential for nuclear integrity and chromosome segregation in mice. Dev Cell 9: 769–779.K. NacerddineF. LehembreM. BhaumikJ. ArtusM. Cohen-Tannoudji2005The SUMO pathway is essential for nuclear integrity and chromosome segregation in mice.Dev Cell9769779
- 14. Nowak M, Hammerschmidt M (2006) Ubc9 regulates mitosis and cell survival during zebrafish development. Mol Biol Cell 17: 5324–5336.M. NowakM. Hammerschmidt2006Ubc9 regulates mitosis and cell survival during zebrafish development.Mol Biol Cell1753245336
- 15. Huang HW, Tsoi SC, Sun YH, Li SS (1998) Identification and characterization of the SMT3 cDNA and gene encoding ubiquitin-like protein from Drosophila melanogaster. Biochem Mol Biol Int 46: 775–785.HW HuangSC TsoiYH SunSS Li1998Identification and characterization of the SMT3 cDNA and gene encoding ubiquitin-like protein from Drosophila melanogaster.Biochem Mol Biol Int46775785
- 16. Lehembre F, Badenhorst P, Muller S, Travers A, Schweisguth F, et al. (2000) Covalent modification of the transcriptional repressor tramtrack by the ubiquitin-related protein Smt3 in Drosophila flies. Mol Cell Biol 20: 1072–1082.F. LehembreP. BadenhorstS. MullerA. TraversF. Schweisguth2000Covalent modification of the transcriptional repressor tramtrack by the ubiquitin-related protein Smt3 in Drosophila flies.Mol Cell Biol2010721082
- 17. Bhaskar V, Valentine SA, Courey AJ (2000) A functional interaction between dorsal and components of the Smt3 conjugation machinery. J Biol Chem 275: 4033–4040.V. BhaskarSA ValentineAJ Courey2000A functional interaction between dorsal and components of the Smt3 conjugation machinery.J Biol Chem27540334040
- 18. Bhaskar V, Smith M, Courey AJ (2002) Conjugation of Smt3 to dorsal may potentiate the Drosophila immune response. Mol Cell Biol 22: 492–504.V. BhaskarM. SmithAJ Courey2002Conjugation of Smt3 to dorsal may potentiate the Drosophila immune response.Mol Cell Biol22492504
- 19. Takanaka Y, Courey AJ (2005) SUMO enhances vestigial function during wing morphogenesis. Mech Dev 122: 1130–1137.Y. TakanakaAJ Courey2005SUMO enhances vestigial function during wing morphogenesis.Mech Dev12211301137
- 20. Savare J, Bonneaud N, Girard F (2005) SUMO represses transcriptional activity of the Drosophila SoxNeuro and human Sox3 central nervous system-specific transcription factors. Mol Biol Cell 16: 2660–2669.J. SavareN. BonneaudF. Girard2005SUMO represses transcriptional activity of the Drosophila SoxNeuro and human Sox3 central nervous system-specific transcription factors.Mol Biol Cell1626602669
- 21. Miles WO, Jaffray E, Campbell SG, Takeda S, Bayston LJ, et al. (2008) Medea SUMOylation restricts the signaling range of the Dpp morphogen in the Drosophila embryo. Genes Dev 22: 2578–2590.WO MilesE. JaffraySG CampbellS. TakedaLJ Bayston2008Medea SUMOylation restricts the signaling range of the Dpp morphogen in the Drosophila embryo.Genes Dev2225782590
- 22. Capelson M, Corces VG (2006) SUMO conjugation attenuates the activity of the gypsy chromatin insulator. Embo J 25: 1906–1914.M. CapelsonVG Corces2006SUMO conjugation attenuates the activity of the gypsy chromatin insulator.Embo J2519061914
- 23. Smith M, Bhaskar V, Fernandez J, Courey AJ (2004) Drosophila Ulp1, a nuclear pore-associated SUMO protease, prevents accumulation of cytoplasmic SUMO conjugates. J Biol Chem 279: 43805–43814.M. SmithV. BhaskarJ. FernandezAJ Courey2004Drosophila Ulp1, a nuclear pore-associated SUMO protease, prevents accumulation of cytoplasmic SUMO conjugates.J Biol Chem2794380543814
- 24. Long X, Griffith LC (2000) Identification and characterization of a SUMO-1 conjugation system that modifies neuronal calcium/calmodulin-dependent protein kinase II in Drosophila melanogaster. J Biol Chem 275: 40765–40776.X. LongLC Griffith2000Identification and characterization of a SUMO-1 conjugation system that modifies neuronal calcium/calmodulin-dependent protein kinase II in Drosophila melanogaster.J Biol Chem2754076540776
- 25. Hashiyama K, Shigenobu S, Kobayashi S (2009) Expression of genes involved in sumoylation in the Drosophila germline. Gene Expr Patterns 9: 50–63.K. HashiyamaS. ShigenobuS. Kobayashi2009Expression of genes involved in sumoylation in the Drosophila germline.Gene Expr Patterns95063
- 26. Denison C, Rudner AD, Gerber SA, Bakalarski CE, Moazed D, et al. (2005) A proteomic strategy for gaining insights into protein sumoylation in yeast. Mol Cell Proteomics 4: 246–254.C. DenisonAD RudnerSA GerberCE BakalarskiD. Moazed2005A proteomic strategy for gaining insights into protein sumoylation in yeast.Mol Cell Proteomics4246254
- 27. Hannich JT, Lewis A, Kroetz MB, Li SJ, Heide H, et al. (2005) Defining the SUMO-modified proteome by multiple approaches in Saccharomyces cerevisiae. J Biol Chem 280: 4102–4110.JT HannichA. LewisMB KroetzSJ LiH. Heide2005Defining the SUMO-modified proteome by multiple approaches in Saccharomyces cerevisiae.J Biol Chem28041024110
- 28. Panse VG, Hardeland U, Werner T, Kuster B, Hurt E (2004) A proteome-wide approach identifies sumoylated substrate proteins in yeast. J Biol Chem 279: 41346–41351.VG PanseU. HardelandT. WernerB. KusterE. Hurt2004A proteome-wide approach identifies sumoylated substrate proteins in yeast.J Biol Chem2794134641351
- 29. Wohlschlegel JA, Johnson ES, Reed SI, Yates JR 3rd (2004) Global analysis of protein sumoylation in Saccharomyces cerevisiae. J Biol Chem 279: 45662–45668.JA WohlschlegelES JohnsonSI ReedJR Yates 3rd2004Global analysis of protein sumoylation in Saccharomyces cerevisiae.J Biol Chem2794566245668
- 30. Zhou W, Ryan JJ, Zhou H (2004) Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses. J Biol Chem 279: 32262–32268.W. ZhouJJ RyanH. Zhou2004Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses.J Biol Chem2793226232268
- 31. Zhao Y, Kwon SW, Anselmo A, Kaur K, White MA (2004) Broad spectrum identification of cellular small ubiquitin-related modifier (SUMO) substrate proteins. J Biol Chem 279: 20999–21002.Y. ZhaoSW KwonA. AnselmoK. KaurMA White2004Broad spectrum identification of cellular small ubiquitin-related modifier (SUMO) substrate proteins.J Biol Chem2792099921002
- 32. Vertegaal AC, Ogg SC, Jaffray E, Rodriguez MS, Hay RT, et al. (2004) A proteomic study of SUMO-2 target proteins. J Biol Chem 279: 33791–33798.AC VertegaalSC OggE. JaffrayMS RodriguezRT Hay2004A proteomic study of SUMO-2 target proteins.J Biol Chem2793379133798
- 33. Manza LL, Codreanu SG, Stamer SL, Smith DL, Wells KS, et al. (2004) Global shifts in protein sumoylation in response to electrophile and oxidative stress. Chem Res Toxicol 17: 1706–1715.LL ManzaSG CodreanuSL StamerDL SmithKS Wells2004Global shifts in protein sumoylation in response to electrophile and oxidative stress.Chem Res Toxicol1717061715
- 34. Rosas-Acosta G, Russell WK, Deyrieux A, Russell DH, Wilson VG (2005) A universal strategy for proteomic studies of SUMO and other ubiquitin-like modifiers. Mol Cell Proteomics 4: 56–72.G. Rosas-AcostaWK RussellA. DeyrieuxDH RussellVG Wilson2005A universal strategy for proteomic studies of SUMO and other ubiquitin-like modifiers.Mol Cell Proteomics45672
- 35. Gocke CB, Yu H, Kang J (2005) Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates. J Biol Chem 280: 5004–5012.CB GockeH. YuJ. Kang2005Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates.J Biol Chem28050045012
- 36. Brand AH, Perrimon N (1993) Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118: 401–415.AH BrandN. Perrimon1993Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.Development118401415
- 37. Saitoh H, Hinchey J (2000) Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3. J Biol Chem 275: 6252–6258.H. SaitohJ. Hinchey2000Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3.J Biol Chem27562526258
- 38. Xue Y, Zhou F, Fu C, Xu Y, Yao X (2006) SUMOsp: a web server for sumoylation site prediction. Nucleic Acids Res 34: W254–257.Y. XueF. ZhouC. FuY. XuX. Yao2006SUMOsp: a web server for sumoylation site prediction.Nucleic Acids Res34W254257
- 39. Brunner E, Ahrens CH, Mohanty S, Baetschmann H, Loevenich S, et al. (2007) A high-quality catalog of the Drosophila melanogaster proteome. Nat Biotechnol 25: 576–583.E. BrunnerCH AhrensS. MohantyH. BaetschmannS. Loevenich2007A high-quality catalog of the Drosophila melanogaster proteome.Nat Biotechnol25576583
- 40. Loevenich SN, Brunner E, King NL, Deutsch EW, Stein SE, et al. (2009) The Drosophila melanogaster PeptideAtlas facilitates the use of peptide data for improved fly proteomics and genome annotation. BMC Bioinformatics 10: 59.SN LoevenichE. BrunnerNL KingEW DeutschSE Stein2009The Drosophila melanogaster PeptideAtlas facilitates the use of peptide data for improved fly proteomics and genome annotation.BMC Bioinformatics1059
- 41. Desterro JM, Rodriguez MS, Kemp GD, Hay RT (1999) Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J Biol Chem 274: 10618–10624.JM DesterroMS RodriguezGD KempRT Hay1999Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.J Biol Chem2741061810624
- 42. Okuma T, Honda R, Ichikawa G, Tsumagari N, Yasuda H (1999) In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2. Biochem Biophys Res Commun 254: 693–698.T. OkumaR. HondaG. IchikawaN. TsumagariH. Yasuda1999In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2.Biochem Biophys Res Commun254693698
- 43. Mencia M, de Lorenzo V (2004) Functional transplantation of the sumoylation machinery into Escherichia coli. Protein Expr Purif 37: 409–418.M. MenciaV. de Lorenzo2004Functional transplantation of the sumoylation machinery into Escherichia coli.Protein Expr Purif37409418
- 44. Uchimura Y, Nakamura M, Sugasawa K, Nakao M, Saitoh H (2004) Overproduction of eukaryotic SUMO-1- and SUMO-2-conjugated proteins in Escherichia coli. Anal Biochem 331: 204–206.Y. UchimuraM. NakamuraK. SugasawaM. NakaoH. Saitoh2004Overproduction of eukaryotic SUMO-1- and SUMO-2-conjugated proteins in Escherichia coli.Anal Biochem331204206
- 45. Uchimura Y, Nakao M, Saitoh H (2004) Generation of SUMO-1 modified proteins in E. coli: towards understanding the biochemistry/structural biology of the SUMO-1 pathway. FEBS Lett 564: 85–90.Y. UchimuraM. NakaoH. Saitoh2004Generation of SUMO-1 modified proteins in E. coli: towards understanding the biochemistry/structural biology of the SUMO-1 pathway.FEBS Lett5648590
- 46. Wohlschlegel JA, Johnson ES, Reed SI, Yates JR 3rd (2006) Improved identification of SUMO attachment sites using C-terminal SUMO mutants and tailored protease digestion strategies. J Proteome Res 5: 761–770.JA WohlschlegelES JohnsonSI ReedJR Yates 3rd2006Improved identification of SUMO attachment sites using C-terminal SUMO mutants and tailored protease digestion strategies.J Proteome Res5761770
- 47. Bridges D, Moorhead GB (2005) 14-3-3 proteins: a number of functions for a numbered protein. Sci STKE 2005: re10.D. BridgesGB Moorhead200514-3-3 proteins: a number of functions for a numbered protein.Sci STKE2005re10
- 48. Su TT, Parry DH, Donahoe B, Chien CT, O'Farrell PH, et al. (2001) Cell cycle roles for two 14-3-3 proteins during Drosophila development. J Cell Sci 114: 3445–3454.TT SuDH ParryB. DonahoeCT ChienPH O'Farrell2001Cell cycle roles for two 14-3-3 proteins during Drosophila development.J Cell Sci11434453454
- 49. Chou TB, Perrimon N (1996) The autosomal FLP-DFS technique for generating germline mosaics in Drosophila melanogaster. Genetics 144: 1673–1679.TB ChouN. Perrimon1996The autosomal FLP-DFS technique for generating germline mosaics in Drosophila melanogaster.Genetics14416731679
- 50. Ray RP, Schupbach T (1996) Intercellular signaling and the polarization of body axes during Drosophila oogenesis. Genes Dev 10: 1711–1723.RP RayT. Schupbach1996Intercellular signaling and the polarization of body axes during Drosophila oogenesis.Genes Dev1017111723
- 51. Schweitzer R, Shilo BZ (1997) A thousand and one roles for the Drosophila EGF receptor. Trends Genet 13: 191–196.R. SchweitzerBZ Shilo1997A thousand and one roles for the Drosophila EGF receptor.Trends Genet13191196
- 52. Van Buskirk C, Schupbach T (1999) Versatility in signalling: multiple responses to EGF receptor activation during Drosophila oogenesis. Trends Cell Biol 9: 1–4.C. Van BuskirkT. Schupbach1999Versatility in signalling: multiple responses to EGF receptor activation during Drosophila oogenesis.Trends Cell Biol914
- 53. Schnorr JD, Holdcraft R, Chevalier B, Berg CA (2001) Ras1 interacts with multiple new signaling and cytoskeletal loci in Drosophila eggshell patterning and morphogenesis. Genetics 159: 609–622.JD SchnorrR. HoldcraftB. ChevalierCA Berg2001Ras1 interacts with multiple new signaling and cytoskeletal loci in Drosophila eggshell patterning and morphogenesis.Genetics159609622
- 54. Friedman A, Perrimon N (2006) A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Nature 444: 230–234.A. FriedmanN. Perrimon2006A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.Nature444230234
- 55. Schweitzer R, Shaharabany M, Seger R, Shilo BZ (1995) Secreted Spitz triggers the DER signaling pathway and is a limiting component in embryonic ventral ectoderm determination. Genes Dev 9: 1518–1529.R. SchweitzerM. ShaharabanyR. SegerBZ Shilo1995Secreted Spitz triggers the DER signaling pathway and is a limiting component in embryonic ventral ectoderm determination.Genes Dev915181529
- 56. Bauer DC, Buske FA, Bodén M (2008) Predicting SUMOylation sites. Lecture Notes in Bioinformatics. Heidelberg: Springer-Verlag. pp. 28–40.DC BauerFA BuskeM. Bodén2008Predicting SUMOylation sites. Lecture Notes in BioinformaticsHeidelbergSpringer-Verlag2840
- 57. Nathan D, Ingvarsdottir K, Sterner DE, Bylebyl GR, Dokmanovic M, et al. (2006) Histone sumoylation is a negative regulator in Saccharomyces cerevisiae and shows dynamic interplay with positive-acting histone modifications. Genes Dev 20: 966–976.D. NathanK. IngvarsdottirDE SternerGR BylebylM. Dokmanovic2006Histone sumoylation is a negative regulator in Saccharomyces cerevisiae and shows dynamic interplay with positive-acting histone modifications.Genes Dev20966976
- 58. Xu J, He Y, Qiang B, Yuan J, Peng X, et al. (2008) A novel method for high accuracy sumoylation site prediction from protein sequences. BMC Bioinformatics 9: 8.J. XuY. HeB. QiangJ. YuanX. Peng2008A novel method for high accuracy sumoylation site prediction from protein sequences.BMC Bioinformatics98
- 59. Dasso M (2008) Emerging Roles of the SUMO Pathway in Mitosis. Cell Div 3: 5.M. Dasso2008Emerging Roles of the SUMO Pathway in Mitosis.Cell Div35
- 60. Freeman M, Nusslein-Volhard C, Glover DM (1986) The dissociation of nuclear and centrosomal division in gnu, a mutation causing giant nuclei in Drosophila. Cell 46: 457–468.M. FreemanC. Nusslein-VolhardDM Glover1986The dissociation of nuclear and centrosomal division in gnu, a mutation causing giant nuclei in Drosophila.Cell46457468
- 61. Raff JW, Glover DM (1988) Nuclear and cytoplasmic mitotic cycles continue in Drosophila embryos in which DNA synthesis is inhibited with aphidicolin. J Cell Biol 107: 2009–2019.JW RaffDM Glover1988Nuclear and cytoplasmic mitotic cycles continue in Drosophila embryos in which DNA synthesis is inhibited with aphidicolin.J Cell Biol10720092019
- 62. Perez-Mongiovi D, Malmanche N, Bousbaa H, Sunkel C (2005) Maternal expression of the checkpoint protein BubR1 is required for synchrony of syncytial nuclear divisions and polar body arrest in Drosophila melanogaster. Development 132: 4509–4520.D. Perez-MongioviN. MalmancheH. BousbaaC. Sunkel2005Maternal expression of the checkpoint protein BubR1 is required for synchrony of syncytial nuclear divisions and polar body arrest in Drosophila melanogaster.Development13245094520
- 63. Goshima G, Wollman R, Goodwin SS, Zhang N, Scholey JM, et al. (2007) Genes required for mitotic spindle assembly in Drosophila S2 cells. Science 316: 417–421.G. GoshimaR. WollmanSS GoodwinN. ZhangJM Scholey2007Genes required for mitotic spindle assembly in Drosophila S2 cells.Science316417421
- 64. Zhang XD, Goeres J, Zhang H, Yen TJ, Porter AC, et al. (2008) SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis. Mol Cell 29: 729–741.XD ZhangJ. GoeresH. ZhangTJ YenAC Porter2008SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis.Mol Cell29729741
- 65. van de Weerdt BC, Medema RH (2006) Polo-like kinases: a team in control of the division. Cell Cycle 5: 853–864.BC van de WeerdtRH Medema2006Polo-like kinases: a team in control of the division.Cell Cycle5853864
- 66. Ghiglione C, Bach EA, Paraiso Y, Carraway KL 3rd, Noselli S, et al. (2002) Mechanism of activation of the Drosophila EGF Receptor by the TGFalpha ligand Gurken during oogenesis. Development 129: 175–186.C. GhiglioneEA BachY. ParaisoKL Carraway 3rdS. Noselli2002Mechanism of activation of the Drosophila EGF Receptor by the TGFalpha ligand Gurken during oogenesis.Development129175186
- 67. Sapir A, Schweitzer R, Shilo BZ (1998) Sequential activation of the EGF receptor pathway during Drosophila oogenesis establishes the dorsoventral axis. Development 125: 191–200.A. SapirR. SchweitzerBZ Shilo1998Sequential activation of the EGF receptor pathway during Drosophila oogenesis establishes the dorsoventral axis.Development125191200
- 68. Schupbach T (1987) Germ line and soma cooperate during oogenesis to establish the dorsoventral pattern of egg shell and embryo in Drosophila melanogaster. Cell 49: 699–707.T. Schupbach1987Germ line and soma cooperate during oogenesis to establish the dorsoventral pattern of egg shell and embryo in Drosophila melanogaster.Cell49699707
- 69. Rebay I (2002) Keeping the receptor tyrosine kinase signaling pathway in check: lessons from Drosophila. Dev Biol 251: 1–17.I. Rebay2002Keeping the receptor tyrosine kinase signaling pathway in check: lessons from Drosophila.Dev Biol251117
- 70. Shilo BZ (2003) Signaling by the Drosophila epidermal growth factor receptor pathway during development. Exp Cell Res 284: 140–149.BZ Shilo2003Signaling by the Drosophila epidermal growth factor receptor pathway during development.Exp Cell Res284140149
- 71. Poulin G, Dong Y, Fraser AG, Hopper NA, Ahringer J (2005) Chromatin regulation and sumoylation in the inhibition of Ras-induced vulval development in Caenorhabditis elegans. Embo J 24: 2613–2623.G. PoulinY. DongAG FraserNA HopperJ. Ahringer2005Chromatin regulation and sumoylation in the inhibition of Ras-induced vulval development in Caenorhabditis elegans.Embo J2426132623
- 72. Hari KL, Cook KR, Karpen GH (2001) The Drosophila Su(var)2–10 locus regulates chromosome structure and function and encodes a member of the PIAS protein family. Genes Dev 15: 1334–1348.KL HariKR CookGH Karpen2001The Drosophila Su(var)2–10 locus regulates chromosome structure and function and encodes a member of the PIAS protein family.Genes Dev1513341348
- 73. Delanoue R, Legent K, Godefroy N, Flagiello D, Dutriaux A, et al. (2004) The Drosophila wing differentiation factor vestigial-scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc. Cell Death Differ 11: 110–122.R. DelanoueK. LegentN. GodefroyD. FlagielloA. Dutriaux2004The Drosophila wing differentiation factor vestigial-scalloped is required for cell proliferation and cell survival at the dorso-ventral boundary of the wing imaginal disc.Cell Death Differ11110122
- 74. Legent K, Dutriaux A, Delanoue R, Silber J (2006) Cell cycle genes regulate vestigial and scalloped to ensure normal proliferation in the wing disc of Drosophila melanogaster. Genes Cells 11: 907–918.K. LegentA. DutriauxR. DelanoueJ. Silber2006Cell cycle genes regulate vestigial and scalloped to ensure normal proliferation in the wing disc of Drosophila melanogaster.Genes Cells11907918
- 75. Martin-Castellanos C, Edgar BA (2002) A characterization of the effects of Dpp signaling on cell growth and proliferation in the Drosophila wing. Development 129: 1003–1013.C. Martin-CastellanosBA Edgar2002A characterization of the effects of Dpp signaling on cell growth and proliferation in the Drosophila wing.Development12910031013
- 76. Barr FA, Sillje HH, Nigg EA (2004) Polo-like kinases and the orchestration of cell division. Nat Rev Mol Cell Biol 5: 429–440.FA BarrHH SilljeEA Nigg2004Polo-like kinases and the orchestration of cell division.Nat Rev Mol Cell Biol5429440
- 77. Shih HP, Hales KG, Pringle JR, Peifer M (2002) Identification of septin-interacting proteins and characterization of the Smt3/SUMO-conjugation system in Drosophila. J Cell Sci 115: 1259–1271.HP ShihKG HalesJR PringleM. Peifer2002Identification of septin-interacting proteins and characterization of the Smt3/SUMO-conjugation system in Drosophila.J Cell Sci11512591271
- 78. Skop AR, Liu H, Yates J 3rd, Meyer BJ, Heald R (2004) Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms. Science 305: 61–66.AR SkopH. LiuJ. Yates 3rdBJ MeyerR. Heald2004Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms.Science3056166
- 79. Fogarty P, Kalpin RF, Sullivan W (1994) The Drosophila maternal-effect mutation grapes causes a metaphase arrest at nuclear cycle 13. Development 120: 2131–2142.P. FogartyRF KalpinW. Sullivan1994The Drosophila maternal-effect mutation grapes causes a metaphase arrest at nuclear cycle 13.Development12021312142
- 80. Riechmann V, Ephrussi A (2001) Axis formation during Drosophila oogenesis. Curr Opin Genet Dev 11: 374–383.V. RiechmannA. Ephrussi2001Axis formation during Drosophila oogenesis.Curr Opin Genet Dev11374383
- 81. Johnstone O, Lasko P (2001) Translational regulation and RNA localization in Drosophila oocytes and embryos. Annu Rev Genet 35: 365–406.O. JohnstoneP. Lasko2001Translational regulation and RNA localization in Drosophila oocytes and embryos.Annu Rev Genet35365406
- 82. Rorth P (1998) Gal4 in the Drosophila female germline. Mech Dev 78: 113–118.P. Rorth1998Gal4 in the Drosophila female germline.Mech Dev78113118
- 83. Studier FW (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr Purif 41: 207–234.FW Studier2005Protein production by auto-induction in high density shaking cultures.Protein Expr Purif41207234
- 84. Sullivan W, Ashburner M, Hawley RS (2000) Drosophila protocols. Sullivan W, Ashburner M, Hawley RS, editors. Cold Spring Harbor Laboratory Press. W. SullivanM. AshburnerRS Hawley2000W. SullivanM. AshburnerRS HawleyDrosophila protocolsCold Spring Harbor Laboratory Press728
- 85. Heuer JG, Li K, Kaufman TC (1995) The Drosophila homeotic target gene centrosomin (cnn) encodes a novel centrosomal protein with leucine zippers and maps to a genomic region required for midgut morphogenesis. Development 121: 3861–3876.JG HeuerK. LiTC Kaufman1995The Drosophila homeotic target gene centrosomin (cnn) encodes a novel centrosomal protein with leucine zippers and maps to a genomic region required for midgut morphogenesis.Development12138613876
- 86. Clemens JC, Worby CA, Simonson-Leff N, Muda M, Maehama T, et al. (2000) Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc Natl Acad Sci U S A 97: 6499–6503.JC ClemensCA WorbyN. Simonson-LeffM. MudaT. Maehama2000Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways.Proc Natl Acad Sci U S A9764996503
- 87. Krishan A (1975) Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J Cell Biol 66: 188–193.A. Krishan1975Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.J Cell Biol66188193