Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

  • Loading metrics

Defining Global Neuroendocrine Gene Expression Patterns Associated with Reproductive Seasonality in Fish

  • Dapeng Zhang ,

    Contributed equally to this work with: Dapeng Zhang, Huiling Xiong

    Affiliation Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ottawa, Ontario, Canada

  • Huiling Xiong ,

    Contributed equally to this work with: Dapeng Zhang, Huiling Xiong

    Affiliation Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ottawa, Ontario, Canada

  • Jan A. Mennigen,

    Affiliation Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ottawa, Ontario, Canada

  • Jason T. Popesku,

    Affiliation Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ottawa, Ontario, Canada

  • Vicki L. Marlatt,

    Affiliation Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ottawa, Ontario, Canada

  • Christopher J. Martyniuk,

    Affiliation Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ottawa, Ontario, Canada

  • Kate Crump,

    Affiliation Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ottawa, Ontario, Canada

  • Andrew R. Cossins,

    Affiliation School of Biological Sciences, University of Liverpool, Liverpool, United Kingdom

  • Xuhua Xia,

    Affiliation Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ottawa, Ontario, Canada

  • Vance L. Trudeau

    trudeauv@uottawa.ca

    Affiliation Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ottawa, Ontario, Canada

Defining Global Neuroendocrine Gene Expression Patterns Associated with Reproductive Seasonality in Fish

  • Dapeng Zhang, 
  • Huiling Xiong, 
  • Jan A. Mennigen, 
  • Jason T. Popesku, 
  • Vicki L. Marlatt, 
  • Christopher J. Martyniuk, 
  • Kate Crump, 
  • Andrew R. Cossins, 
  • Xuhua Xia, 
  • Vance L. Trudeau
PLOS
x

Abstract

Background

Many vertebrates, including the goldfish, exhibit seasonal reproductive rhythms, which are a result of interactions between external environmental stimuli and internal endocrine systems in the hypothalamo-pituitary-gonadal axis. While it is long believed that differential expression of neuroendocrine genes contributes to establishing seasonal reproductive rhythms, no systems-level investigation has yet been conducted.

Methodology/Principal Findings

In the present study, by analyzing multiple female goldfish brain microarray datasets, we have characterized global gene expression patterns for a seasonal cycle. A core set of genes (873 genes) in the hypothalamus were identified to be differentially expressed between May, August and December, which correspond to physiologically distinct stages that are sexually mature (prespawning), sexual regression, and early gonadal redevelopment, respectively. Expression changes of these genes are also shared by another brain region, the telencephalon, as revealed by multivariate analysis. More importantly, by examining one dataset obtained from fish in October who were kept under long-daylength photoperiod (16 h) typical of the springtime breeding season (May), we observed that the expression of identified genes appears regulated by photoperiod, a major factor controlling vertebrate reproductive cyclicity. Gene ontology analysis revealed that hormone genes and genes functionally involved in G-protein coupled receptor signaling pathway and transmission of nerve impulses are significantly enriched in an expression pattern, whose transition is located between prespawning and sexually regressed stages. The existence of seasonal expression patterns was verified for several genes including isotocin, ependymin II, GABAA gamma2 receptor, calmodulin, and aromatase b by independent samplings of goldfish brains from six seasonal time points and real-time PCR assays.

Conclusions/Significance

Using both theoretical and experimental strategies, we report for the first time global gene expression patterns throughout a breeding season which may account for dynamic neuroendocrine regulation of seasonal reproductive development.

Introduction

Fundamental to the survival of most organisms, be they yeast, plants, fishes, or mammals, are biological rhythms with periodic (daily, monthly or annual) changes in behaviour and physiology [1]. The daily circadian rhythm is exemplified by opening/closing of flowers or the daily sleep cycle in humans. The menstrual cycle of women is a typical monthly rhythm whereas circannual rhythms include bird migrations, hibernation in frogs and mammals. Marked reproductive seasonality in numerous vertebrate classes, including fish, ensures that reproduction and subsequent development of offspring is coordinated with optimal environmental and nutritional conditions. It has long been accepted that external environmental influences such as photoperiod [2], [3], [4] and temperature [4], [5], [6] exert dominant roles in biological rhythms, and internal neuroendocrine systems such as the pineal gland, hypothalamus and pituitary coordinate these signals [7], [8], [9], [10]. In this study, we use theoretical and experimental approaches to better understand global genomic regulation of the neuroendocrine system during seasonal reproduction.

The bony fishes or teleosts represent more than half of all vertebrates. Numerous characteristics of goldfish (Carassius auratus), a member of the family Cyprinidae, one of the largest vertebrate families, make this species an excellent model for neuroendocrine signaling and the control of seasonal reproduction [11], [12]. Similar to many teleosts, goldfish employ a seasonal reproductive strategy [5] characterized by distinct stages. In temperate regions of the Northern hemisphere the exact timing of these stages in goldfish depends on geographical location but can be generally classified as follows: gonadal regression (June-October), recrudescence or redevelopment (October-March), prespawning (April-May) and spawning or breeding season (May or early June).

Seasonal reproductive cyclicity including annual regeneration of gonadal tissues is mediated by a hormone regulatory pathway primarily involving gonadotropin-releasing hormone (GnRH), luteinizing hormone (LH), FSH, growth hormone (GH), melatonin, and sex steroid hormones [8], [9], [10]. The main hypophysiotrophic systems controlling LH and GH are located in the hypothalamus and the preoptic region of the telencephalon, which have been well-described and characterized in goldfish [8], [13]. Transduction of photoperiodic signals via retino-hypothalamo-pineal pathways results in melatonin secretion patterns typically reflecting the length of the dark period [14], [15], [16]. Melatonin influences the neuroendocrine system via its receptors which are widely distributed in the teleost brain [17], [18], [19]. Multiple GnRH forms [20] activate anterior pituitary receptors to stimulate the expression and release of LH, follicle-stimulating hormone (FSH) and GH, which are all involved in controlling seasonal gonadal development and sex steroid synthesis. Moreover, synchronization of fish reproductive behaviours in the breeding season involves a well-defined sex pheromone response system, culminating in surge release of LH that induces ovulation in females and sperm release in males [21]. Testosterone (T) and estrogen (E2) exert a primary role in gonadal development locally, and have profound positive and negative feedback actions at the levels of the brain and pituitary [8]. In addition to these core members of hypothalamo-pituitary-gonadal (HPG) axis, there is complex and interactive regulation of GnRH and LH/FSH by a multitude of neuroendocrine factors including classical neurotransmitters and other neuropeptides [8], [9], [10], [12], [22]. Glutamate, gamma-aminobutyric acid (GABA), and a multitude of neuropeptides are predominantly stimulatory on LH release whereas dopamine is the single most potent inhibitor of LH release in teleosts [8], [12], [22].

It has been extensively described that reproductive seasonality in teleosts is characterized by their seasonal profiles or changes in serum hormone levels [23], [24], [25], brain hormone content [26], brain enzyme activities [23], morphological [27] and histological [28] phenotypes. More recently, there is recognition that seasonal expression of the neuroendocrine genes including neurohypophysial hormones, synthesis enzymes, and related signaling pathway proteins may be utilized to link the external environmental influences and the establishment of fish reproductive cyclicity. It has been observed that in teleosts many neuroendocrine genes including aromatase b [29], GnRH receptors [30], glutamic acid decarboxylase (GAD) [31], cholecystokinin (CCK) [32], preprotachykinin [33] and secretogranin-II [34] have seasonal expression profiles, correlating with seasonal changes in gonadal size and sex steroid serum levels [23], [24], [35], [36]. However, no study at the transcriptomic level has been conducted to investigate the global gene expression changes in fish neuroendocrine systems throughout an annual reproductive cycle. We previously utilized microarrays to identify hormone or endocrine disrupting chemical-regulated genes in the goldfish neuroendocrine system [37], [38], [39], [40]. All experiments were based on goldfish sampled at different seasonal time points; thus, these microarray datasets contain important seasonal gene expression information (Table S1). In the present study, on the basis of multiple goldfish microarray datasets, we used comprehensive normalizations, differential gene expression identification, multivariate and gene ontology (GO) analyses to characterize the season-related expression patterns in the female goldfish brain. A core gene set was identified whose expression exhibits a seasonal change with fish reproductive cycle. We illustrate that the gene expression change is regulated by photoperiod. Gene ontology (GO) analysis further revealed that neuroendocrine-related genes are significantly enriched in an expression pattern, which follows a high-low-low profile along the spring-summer-winter seasonal axis. This pattern was confirmed by a series of independent seasonal brain sampling and real-time PCR verification on five important neuroendocrine genes. We hypothesize that the seasonal expression patterns may account for dynamic neuroendocrine regulation of reproductive cyclicity.

Results

Extracting neuroendocrine brain gene expression information from multiple female goldfish microarray datasets

Experimental microarray data from the AURATUS goldfish environmental genomics project were used to define seasonal gene expression information (Table S1). In order to remove systemic bias and random variation within and between different experiments, we conducted a series of data transformation and normalization procedures. For each experiment, we first subjected the slides to Lowess and Scale normalizations. The raw control sample intensity was extracted and used to represent transcriptomic status of brain samples in different seasons. We further removed the bias between different experiments using Quantile normalization. This normalization procedure was firstly used for the slides (n = 24) with an mRNA source from female hypothalamus (Hyp), and Figure 1 shows data distribution for all slides during normalizations. Datasets can be divided into three seasonal time points (May, August, and December), which correspond to the distinct physiological periods of sexually mature prespawning, sexually regressed, and early redevelopment of goldfish ovary, respectively.

thumbnail
Figure 1. Boxplots of M values showing data distribution during the normalization procedures.

https://doi.org/10.1371/journal.pone.0005816.g001

The global transcriptomic relationships among slides were evaluated by principal component analysis (PCA). PCA is a dimension-reducing multivariate statistical technique for simplifying complex datasets [41], [42], [43]. The results of the PCA are visualized in a two-dimensional plot. This sub-space captures the highest amount of total variability. The points in the plots represent the global transcriptomes of the different slides. The transcriptomes that are clustered together are considered overall to be similar, while more dissimilar transcriptomes are further apart. As shown in Figure S1, although from different experiments, global transcriptomes of the Hyp slides from a given season are closely clustered together. This indicates that our normalization procedure was effective in identifying the season-dependent gene expression information in these microarray datasets.

Differentially expressed genes in female hypothalamus along the reproductive seasonal cycle

We identified the differentially expressed genes between these three seasonal time points. The optimal discovery procedure (ODP) method [44] implemented in an EDGE program [45] was utilized for this purpose. A total of 873 unique differentially expressed genes were identified with high statistical significance (q value<0.0001) (Table S2). These differential genes constitute about 10% total genes (8448 genes) represented on the arrays and include 662 genes whose function is at least partially characterized and 211 unidentified EST sequences. All of the genes were further subjected to hierarchical cluster analysis (HCA) using Pearson correlation as a distance function. In the HCA, not only the relationships between different samples can be classified, but also the genes with similar expression patterns can be grouped by visual inspection of the hierarchical cluster results. As shown in Figure 2, the identified differentially expressed genes fall into four gene expression clusters comprising H-L-L, H-H-L, L-H-H and L-L-H (L, relatively low expression and H, relatively high expression) patterns along the seasonal cycle (May-August-December). Most of the identified genes belong to H-L-L and L-H-H expression clusters.

thumbnail
Figure 2. Hierarchical clustering of expression profiles of significantly differentially expressed genes between three reproductive seasonal time points (May, August, and December).

Signal represents log ratio intensity of the gene expression. Red indicates relatively high expression and blue indicates relatively low expression.

https://doi.org/10.1371/journal.pone.0005816.g002

Telencephalon exhibits similar transcriptomic patterns as hypothalamus

We next examined the global transcriptome similarity between Hyp and Tel during the seasonal cycle. Both Hyp and Tel are important brain regions involved in neuroendocrine control of growth and reproduction (Figure 3a). Five expression datasets (n = 20) for female Tel in May and August were available for this study. After the previously applied data normalizations, PCA was performed for both Hyp and Tel datasets. The output of PCA showed that transcriptomes of Hyp and Tel samples in both May and August are overlapped (Figure 3b). This indicates that these tissues have highly similar gene expression profiles in the same season (e.g. in May and August).

thumbnail
Figure 3. Similar transcriptomic patterns in both hypothalamus and telencephalon.

(a) Location of hypothalamus (Hyp) and telencephalon (Tel) in goldfish brain. (b) Two-dimensional PCA plot for transcriptomes of both Hyp and Tel slides in three seasonal time points (May, August, and December). Eigenvalues of PCA are listed in Table S3. (c) Hierarchical clustering of the expression profiles of the significantly differentially expressed genes in Hyp and Tel along the seasonal cycle.

https://doi.org/10.1371/journal.pone.0005816.g003

We further investigated whether the expression patterns of differentially expressed genes identified in Hyp are similar in the Tel. HCA was carried out on a combined data set including the expression values of all differential genes from Tel slides and those from the Hyp slides. We found that as in Hyp, those genes also exhibit clear differential expression patterns in Tel between May and August (Figure 3c). This was also observed by clustering global transcriptomes of both Hyp and Tel (not shown). Therefore, using multivariate analysis we illustrate that the transcriptomes from two neuroendocrine brain regions (Hyp and Tel) that are controlling reproduction are surprisingly similar within a given season and the differential gene patterns identified in Hyp was also evident in Tel.

The identified gene expression patterns can be regulated by photoperiod

Photoperiod is a major environmental factor that governs fish reproductive seasonality [3], [4], [5], [46]. Photoperiodic manipulations have been used to control the egg production and fertilization success of diverse species such as goldfish [47], [48], rainbow trout (Salmo gairdneri) [49], Japanese medaka (Oryzias latipes) [6] and European sea bass (Dicentrarchus labrax L.) [3]. Moreover, some photoperiod-regulated genes have also been described in the ovine pituitary gland [50] and the hamster hypothalamus [51]. Therefore, it can be hypothesized that the identified gene signature which is differentially expressed between seasons is regulated by photoperiod. One dataset provided a unique opportunity to clarify this question in which Hyp brain samples were taken from fish in October that had been acclimated to a long-daylength photoperiod (16 h), typical of the springtime breeding season in May (Figure 4a). The prediction was that if the identified gene expression signature is photo-responsive, the genes in 16 h-October fish should have similar expression patterns to fish in May acclimated for 3 weeks under our seasonally varying daylength protocol and sampled at 15.8 h light-length.

thumbnail
Figure 4. Regulation of brain gene expression patterns by photoperiod.

(a) Seasonal profile of the daylength in Ottawa, Canada (45°27′N, 75°42′W). Pink, blue, grey and red boxes indicate daylength in May, August, October and December, respectively. The light blue box indicates a 16-hour daylength that we used for one fish group in October. Green bar indicates breeding season for goldfish typical in temperate North America. (b) Two-dimensional PCA plot for transcriptomes from Hyp slides in four seasonal time points (May, August, 16 h-October, and December). (c) Hierarchical clustering of expression profiles of significantly differentially expressed genes in Hyp slides along four seasonal time points.

https://doi.org/10.1371/journal.pone.0005816.g004

We first examined the relationship of the transcriptomes between the 16 h-October Hyp samples and other three normal Hyp samples using PCA. As shown in Figure 4b, the data points of 16 h-October slides are closely clustered with May slides, indicating similar global transcriptome status between the May and 16 h-October groups. We also compared the gene expression relationship of the identified differentially expressed genes in these four cases. As shown in Figure 4c, the expression of these 873 genes highly resembles that observed in May. Moreover, the gene expression patterns are reversed in 16 h-October compared to other seasons August and December. When we combined this dataset for a re-analysis to identify differential genes between seasons, we obtained a very similar gene list and gene patterns (data not shown). Therefore, we concluded that the identified differential expression patterns of these genes are likely regulated by photoperiod.

Gene ontology analysis of differentially expressed genes

We have shown that a core set of genes were differentially expressed between seasons in both Hyp and Tel regions and they are photoperiod-responsive. We hypothesize that the affected genes should be related to neuroendocrine regulation of the fish reproductive cyclicity. We utilized GO term analysis to explore such potential functional significance among these genes. A series of GO categories implicated in neuroendocrine function are significantly over-represented for the genes of the H-L-L pattern (Figure S2). Figure 5 shows the primary GO categories with member genes, some of which can be found in multiple categories. Importantly, many genes have been functionally assigned to hormone activity, including two GnRH forms (salmon GnRH and chicken GnRH-II), growth hormone (GH), isotocin, LH, tachykinin, neuropeptide Y (NPY), pituitary adenlyate cyclase activating polypeptide 1 beta (PACAP 1β). Most of them, especially the GnRHs, are well-known and central to neuroendocrine control of reproduction. Another identified molecular function is GABAA receptor activity. Four subunits, GABAA alpha1, beta4, gamma1 and gamma2, were highly expressed in May. The GABAA receptor is a pentameric protein that is a ligand-gated Cl ion channel whose activation by GABA or the specific GABAA agonist muscimol results in rapid and sustained LH release in goldfish [52]. Many other genes identified are involved in several biological processes including: transmission of nerve impulse, glutamine family amino acid metabolic process, G-protein coupled receptor (GPCR) signaling pathway and neuron differentiation. Some genes are shared between these biological process and molecular function categories, among which transmission of nerve impulse is a central core linking other categories. Successful identification of a series of neuroendocrine function-related genes by GO enrichment analysis supports the biological implication of gene expression change of the H-L-L (May-August-December) gene pattern in reproductive seasonality. Moreover, since the timing of major expression change corresponds to the transition from prespawning/spawning to sexually regressed female goldfish, the high expression status of these genes around May, therefore, should be required for the fish spawning period.

thumbnail
Figure 5. The neuroendocrine genes which are significantly enriched in the H-L-L expression pattern and their associated Gene Ontology categories.

Gene abbreviations: CaM, calmodulin; CaMK2δ, calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; Car-D, carnosine dipeptidase 1; CCK, cholecystokinin; cGnRH-II, chicken gonadotropin-releasing hormone II; Ckb, creatine kinase, brain; GABAA α1, β4, γ1 and γ2, GABAA receptor alpha1, beta4, gamma1 and gamma2; GABA:Na, GABA:Na symporter; GAD3, glutamate decarboxylase isoform 3; GH, growth hormone; GLP1R, Glucagon-like peptide-1 receptor; GluL α, glutamate-ammonia ligase alpha; GluR2, glutamate receptor, metabotropic 2; HMP19, neuron specific gene family member 2 (protein p19); LH, luteinizing hormone; NPY, Neuropeptide Y; NSF, n-ethylmaleimide-sensitive factor; PACAP 1β, pituitary adenlyate cyclase activating polypeptide 1 beta; PACAP 1R, PACAP type 1 receptor; Pro-D, Proline dehydrogenase; Rab14, member 14 of RAS oncogene family; sGnRH, salmon gonadotropin-releasing hormone; β-Synuclein, synuclein beta; TAU, microtubule-associated protein tau; UchL1, ubiquitin carboxyl-terminal esterase L1 (also called ubiquitin thiolesterase); VDAC1, voltage-dependent anion channel 1; YWHAG, 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 2.

https://doi.org/10.1371/journal.pone.0005816.g005

Seasonal goldfish brain sampling and real-time RT-PCR verifications

Following bioinformatics analysis, we collected goldfish brain samples at six seasonal time points for experimental verification of gene expression patterns. Gonadosomatic index (GSI), an indication of the sexual maturity of the fish, shows variation along the reproductive season and reaches a peak around May (Figure 6a). The observed changes are comparable to previous studies in Japan [35] and Canada [24]. We used real-time RT-PCR to examine the seasonal expression profiles of five genes: isotocin, GABAA gamma2, calmodulin, ependymin II and aromatase b. Among these genes, isotocin, GABAA gamma2 and calmodulin are enriched in GO term analysis (Figure 5). Isotocin is a nine amino acid neuropeptide whose function is related to the control of reproductive and social behaviours in teleost fish [53], [54]. The effects on LH release by its mammalian homologue, oxytocin, was also reported in mammalian models [55], [56]. GABAA gamma2 is one subunit of GABAA receptor complex that is involved in binding of GABAA receptor modulators such as benzodiazepines [57]; it is a representative of GABA system genes. Calmodulin is a calcium ion-binding protein and has diverse regulatory roles in L-type calcium channel, signal transduction, synthesis and release of neurotransmitters [58], and transcriptional action of estrogen receptors [59]. Ependymin II is a brain glycoprotein and is implicated in neuronal regeneration [60]. Aromatase b is the brain type aromatase, a P450 family enzyme expressed exclusively in teleost radial glial cells [61], and contributes to catalyzing the aromatization of testosterone (T) to 17beta-estradiol (E2). Although aromatase b is not identified by our meta-analysis, we are interested in it because both T and E2 have regulatory effect on the neuroendocrine system and the seasonal activity of aromatase b in fish brain is known to correlate significantly with seasonal reproductive cycles [23].

thumbnail
Figure 6. Seasonal profiles in GSI values and brain gene expression in female goldfish.

(a) GSI values of female goldfish. (b)–(f) relative gene expression of neuroendocrine genes in female goldfish Hyp along a whole seasonal cycle. The green bar indicates the typical breeding period for goldfish in temperate North America.

https://doi.org/10.1371/journal.pone.0005816.g006

As shown in Figure 6b–f, seasonal expression profiles of these five genes in Hyp exhibit a clear increased expression in May. Significant differences (P<0.05; Holm-Sidak multiple test) can also be observed for the expression of most genes between May and other seasons. The range of overall fold changes for those genes (Figure 6) is similar to microarray analysis (Figure 2). Therefore, these results strongly support the patterns found in our meta-analysis of microarray data with an apparent expression peak in May representing the prespawning season of the goldfish.

Discussion

An emerging concept about neuroendocrine regulation of biological rhythms is the recognized importance of the seasonal gene expression profiles in the neuroendocrine brain. Although several elegant studies have attempted to define gene expression change associated with hibernation in whole squirrel brain [62], migration in sparrow telencephalon [63] or photo-response in hamster hypothalamus [51], no system-level study has been conducted to profile the global gene expression changes along a whole reproductive cycle. The purpose of this study was to extract and evaluate such seasonal gene expression characteristics from the existing multiple microarray datasets using the goldfish model. A series of normalization procedures were utilized to remove systematic and experimental biases. The overall similarity among the slides from the same season as determined with PCA indicates that this strategy is effective. On the basis of this meta-type dataset, we identified four clear seasonal gene expression patterns in female goldfish brain. Interestingly, approximately 90% of genes represented on the array exhibit no detectable variation between the examined seasons. This suggests that the fish brain is a relatively stable system. The array does not cover the full transcriptome, but many biological pathways and processes are represented and they did not exhibit any detectable seasonal variation. In contrast, a set of genes were found differentially expressed between seasons and most of them fall into two distinct expression patterns (H-L-L and L-H-H). We observed that both female Hyp and Tel have a similar transcriptome status and same set of genes are differentially expressed between seasons. We further illustrated that artificially increasing daylength in October (16 h light) resulted in an expression profile in Hyp very similar to that of female goldfish in May (15.8 h light). This supports the hypothesis that photoperiod is a major environmental cue for vertebrate reproduction [4] because gene expression profiles in the neuroendocrine brain were photoperiodically-regulated to some degree.

Most differential genes showed expression changes between May and August in H-L-L and L-H-H patterns. May and August correspond to fish reproductive status of sexually mature prespawning and sexual regression respectively, and histological (ovary) and serum hormone changes have been extensively described between these two stages [23], [24], [28]. Thus, expression transition of this core gene set in the neuroendocrine brain may provide the molecular basis for phenotypic changes in the pituitary gland, circulating serum hormone levels and gonad status between seasons. Indeed, a series of GO categories related to neuroendocrine function and process are significantly overrepresented for the genes of H-L-L pattern (Figure 5). Many associated genes have been shown directly involved in regulating brain function and seasonal reproduction, as exemplified by the neuropeptides GnRH, isotocin, NPY and CCK. Moreover, other biological processes have also been identified including glutamate/GABA systems, GPCR signaling pathway and transmission of nerve impulse. Although no GO category is significantly enriched for the genes of L-H-H pattern, we speculate that they may include some inhibitory factors for neuroendocrine function such as the dopamine system related genes [12]. Other potential functions of these L-H-H genes may be involved in the marked seasonal growth phase of goldfish, which is different from reproductive phase [8]. We further verified the seasonal expression profiles for five genes which represent the GABA system (GABAA gamma2), estrogen production (aromatase b), peptide hormones (isotocin), calcium binding proteins (ependymin II and calmodulin) and GPCR signaling pathway (calmodulin). Their seasonal expression profiles all exhibit similar predicted patterns with the apparent peak around May, thereby suggesting that a seasonal expression change is employed by their represented systems (e.g. E2 and GABA) to coincide with fish reproductive development.

Our study may serve as a basis for understanding reproductive seasonality of other fish species. Seasonal patterns in serum levels of reproductive hormones has been documented in many teleost species (e.g., Florida gar (Lepisosteus platyrhincus) [36], masu salmon (Onchorhynchus masou) [64], and European sea bass [25]), including the goldfish [35]. It can be hypothesized that although different fish species have their own reproductive strategies and particular seasonal pattern, they may share one core set of genes with an increased expression prior to the spawning period. Indeed, similar increased expression pattern of several neuroendocrine genes has been observed in other fish species. For example, Okuzawa and colleagues [65] have shown that GnRHs exhibit a similar seasonal expression pattern in the brain of female red seabream (Pagrus major) with an expression peak around April, correlating with high steroid level and mature gonad state. Increased expression of GnRHs can also be found in the turbot (Scophthalmus maximus) [66], in parallel with an increase in oocyte diameter. Aromatase is another example; in the midshipman fish (Porichthys notatus), aromatase b in the magnocellular preoptic area of the brain showed higher expression in the pre-nesting period when compared to nesting and non-reproductive periods [67]. Moreover, in both red-spotted grouper (Epinephelus akaara) [68] and in the European sea bass [25], aromatase b showed enhanced activity during their own prespawning periods, which are corresponding to April and January, respectively.

Overall, using both bioinformatic and experimental strategies, we defined a core gene set whose expression exhibits a seasonal change along a fish reproductive cycle and illustrated that the gene expression change is primarily regulated by photoperiod. Moreover, most genes undergo an expression transition between May and August, which corresponds to the transition from prespawning to sexual regression. Thus the gene expression patterns may account for dynamic neuroendocrine regulation on fish seasonal reproductive development and the increased expression of these neuroendocrine related genes in May is crucial for ensuing spawning period. This is all the more critical in species with a short breeding period or where seasonally timed spawning occurs only once in the year.

Materials and Methods

Ethics statement

The care and treatment of animals used in this study were in accordance with the guidelines of the Animal Care Committee, University of Ottawa and the Canadian Council on Animal Care.

Experiments and microarray datasets

All gene expression data were from version 1.0 Carp-Goldfish arrays which were printed in at University of Liverpool Microarray Facility, UK. The Gene Expression Omnibus (GEO) database accession number of the microarray platform is GPL3735. A total of 48 cDNA microarray slides were used for this study, and each slide was comprised of 6119 unique gene sequences and 2329 EST sequences printed in duplicate on each slide. Microarray datasets of gene expression in female goldfish hypothalamus and telencephalon have been collected to represent four seasonal time points. Detailed information about the experimental design, brain tissue, sampling seasonal time, temperature, daylength and other information are listed in Table S1. Some original results of corresponding experiments have been published [37], [39], and others will be published elsewhere. The hybridization, scanning procedures and validation of microarrays has been described previously by our group [37].

Data normalizations

For each array, spots that had been manually flagged due to poor hybridization and spots in which the estimated fluorescence intensity was below or equal to the estimated background signal intensity in either channel were removed before further analysis. Lowess normalization [69] with span 0.4 was used to decrease the intensity dependent biases within slide, followed by Scale normalization [69]. Thereafter, control sample intensities were recovered and extracted to build a new series of expression data for all the slides. Following this, Quantile normalization [70] was used for across-slide normalization to minimize biases among different experiments.

Identification of differentially expressed genes

An optimal discovery procedure (ODP) method implemented in the Extraction of Differential Gene Expression (EDGE) program [44] was used to assess the significance of differential expression of the genes between different seasonal time points. The false discovery rate for selected genes was monitored and controlled by calculating the q value. Genes with q value<0.0001 were considered significantly differentially expressed genes between comparison groups.

Multivariate data analysis

Two multivariate methods including principal component analysis (PCA) and hierarchical clustering analysis (HCA) were utilized in this study. PCA was used to examine the transcriptome similarity relationship among slides that correspond to different seasonal time points. HCA was used to classify/cluster the gene expression patterns. Briefly, PCA combines two or more correlated factors (i.e. transcripts) into one new variable, a principal component (PC) [41], [42], [43]. In PCA the dimensionality of the dataset is reduced by replacing the original variables by a smaller number of newly formed variables that are linear combinations of the original variables and that explain the majority of the information (variability) from the experiment. Eigenvalues of PCA can be found in Table S3.

A hierarchical clustering tree [71] generates a binary dendrogram representing the association structure of pairs of arrays or genes, which was measured in terms of the Pearson correlation of the standardized intensity measurements. The algorithm identifies the gene pair with the smallest distance and groups them with a link, where distance is defined to be one-minus the correlation. The algorithm proceeds in a recursive manner to build the tree structure step by step. PCA and HCA were conducted by the MultiExperiment Viewer (MeV) [72] and EDGE [44] programs.

Gene Ontology analysis

The resulting differentially expressed genes from each pattern were submitted to the Blast2GO web server [73]. Enrichment of multiple-level GO functional categories was determined using the GOSSIP/Fisher Exact test package [74], through a comparison of the categories found in our list of genes compared to the list of all genes found on the goldfish cDNA microarray. FDR controlled p-values (FDR<0.05) were used for the assessment of functional significance.

Animal husbandry and tissue sampling

Adult female goldfish (Carassius auratus) were purchased from Aleong's International Inc. (Mississauga, ON, Canada), acclimated for 3 weeks to 18°C under a natural seasonally variable simulated photoperiod except for the animals sampled in October. In this case, animals were acclimated to 18°C and 16 h daylength that is typical of springtime. Fish were fed and maintained on standard flaked goldfish food. For the seasonal profile, 18 female fish were collected every two months starting in January, for a total of six time points (January, March, May, July, September, and November). Goldfish were anesthetized using 3-aminobenzoic acid methylester (MS222; Aquatic Eco-Systems, Apopka, FL) for all handling and dissection procedures. Care was taken to standardize all handling and sample protocols. Goldfish were sacrificed by spinal transection and 3 hypothalami were pooled for a total n = 6. Samples were rapidly dissected and immediately frozen on dry ice. At the time of sampling, body weights and gonadal weights were recorded and the gonadosomatic index (GSI; body weight/gonad weight ×100) was calculated.

RNA extraction, quality assessment and cDNA synthesis

RNA was isolated using the RNeasy Plus Mini kit (Qiagen) as described in the manufacturer's protocol. Briefly, brain samples were homogenized using the stainless steel beads before using the RNeasy Plus Mini kit. Upon purification, concentration and quality of all samples was assessed using the 2100 Bioanalyzer (Agilent). RIN values of RNA samples ranged from 7 to 9.5, which is above the recommended minimum value of 5 for quantitative real-time RT PCR applications [75]. Total cDNA was prepared from 1 µg total RNA and 200 ng random hexamer primers (Invitrogen) using Superscript II RNase H- reverse transcriptase (SSII) as described by the manufacturer (Invitrogen). Each 20 µl reaction was diluted 10-fold in nuclease-free water and used as the template for the real-time RT-PCR assays. All brain cDNA samples from different seasonal time points were synthesized in parallel.

Real-time RT-PCR verification

SYBR green real-time RT-PCR assay was used to validate relative expression of five genes (isotocin, GABAA gamma2, calmodulin, ependymin II and aromatase b) at all six time points along the reproductive cycle. Primers (Table S4) were designed using Primer3 [76] and synthesized by Invitrogen. The Mx3000 Quantitative PCR System (Stratagene, La Jolla, CA) was used to amplify and detect the transcripts of interest. Each real-time RT-PCR reaction contained the following final concentrations: 25 ng first strand cDNA template, 1× QPCR buffer, 3.5 mM MgCl2, optimized concentrations (150 nM–300 nM) of gene-specific primers, 0.25× SYBRGreen (Invitrogen), 200 µM dNTPs, 1.25U HotStarTaq (Invitrogen), and 100 nM ROX reference dye, in a 25 µL reaction volume. The thermal cycling parameters were an initial 1 cycle Taq activation at 95°C for 15 min, followed by 40 cycles of 95°C for 15 s, optimized annealing temperature (58–60°C) for 5 s, 72°C for 30 s, and a detection step at 80°C for 8 s. Dilutions of cDNA (1∶10 to 1∶31,250) from all samples were used to construct a relative standard curve for each primer set. After the reaction was complete, a dissociation curve was produced starting from 55°C (+1°C/30 s) to 95°C. For each PCR reaction, negative controls were also introduced including a no template control (NTC) where RNase-free water was added to the reaction instead of the template (cDNA) and a no reverse transcriptase control (NRT) where RNase-free water was added to the cDNA synthesis reaction (previously described) instead of the SSII enzyme. The SYBR green assay for every target gene was optimized for primer concentration and annealing temperature to obtain a dRn value higher than 0.98, an amplification efficiency between 90%–110% and a single sequence-specific peak in the dissociation curve.

Data were analyzed using the MxPro software package. The relative standard curve method was used to calculate relative mRNA abundance between samples, which was then presented as means+SEM of gene expression from six biological replicates (assayed in duplicate) for each time point. A one-way analysis of variance (ANOVA) followed by a Holm-Sidak post-hoc test in SigmaStat3.5 (SPSS Inc.) was used to evaluate significant changes in gene expression between different time points. All data were first tested for normality and those data with non-normal distribution were subjected to square root transformations prior to statistical analyses.

Supporting Information

Figure S1.

Two-dimensional PCA plot for transcriptomes from Hyp slides in three seasonal time points (May, August, and December).

https://doi.org/10.1371/journal.pone.0005816.s001

(0.08 MB PPT)

Figure S2.

Gene ontolgy (GO) term enrichment analysis for the genes with H-L-L pattern.

https://doi.org/10.1371/journal.pone.0005816.s002

(0.28 MB PPT)

Table S1.

Detailed information about the experimental design, brain tissue, sampling seasonal time and other information.

https://doi.org/10.1371/journal.pone.0005816.s003

(0.04 MB DOC)

Table S2.

A full list of differentially expressed genes between seasons.

https://doi.org/10.1371/journal.pone.0005816.s004

(0.65 MB XLS)

Table S4.

Oligonucleotide primers used for real-time RT-PCR assays.

https://doi.org/10.1371/journal.pone.0005816.s006

(0.03 MB DOC)

Acknowledgments

The collaboration of Dr. Donald E. Tillitt (USGS) is acknowledged. We also thank Dr. Margaret Hughes (Liverpool Microarray Facility, UK) for microarray production and assistance.

Author Contributions

Conceived and designed the experiments: DZ HX VLT. Performed the experiments: DZ HX JAM VLM. Analyzed the data: DZ HX JTP. Contributed reagents/materials/analysis tools: JAM JTP CJM KC ARC XX. Wrote the paper: DZ HX VLT.

References

  1. 1. Ripperger JA (2007) The rhythms of life. Genome Biol 8: 313.JA Ripperger2007The rhythms of life.Genome Biol8313
  2. 2. Davis SJ, Millar AJ (2001) Watching the hands of the Arabidopsis biological clock. Genome Biol 2: REVIEWS1008.SJ DavisAJ Millar2001Watching the hands of the Arabidopsis biological clock.Genome Biol2REVIEWS1008
  3. 3. Bayarri MJ, Rodriguez L, Zanuy S, Madrid JA, Sanchez-Vazquez FJ, et al. (2004) Effect of photoperiod manipulation on the daily rhythms of melatonin and reproductive hormones in caged European sea bass (Dicentrarchus labrax). Gen Comp Endocrinol 136: 72–81.MJ BayarriL. RodriguezS. ZanuyJA MadridFJ Sanchez-Vazquez2004Effect of photoperiod manipulation on the daily rhythms of melatonin and reproductive hormones in caged European sea bass (Dicentrarchus labrax).Gen Comp Endocrinol1367281
  4. 4. Peter RE, Crim LW (1979) Reproductive endocrinology of fishes: gonadal cycles and gonadotropin in teleosts. Annu Rev Physiol 41: 323–335.RE PeterLW Crim1979Reproductive endocrinology of fishes: gonadal cycles and gonadotropin in teleosts.Annu Rev Physiol41323335
  5. 5. Blazquez M, Bosma PT, Fraser EJ, Van Look KJ, Trudeau VL (1998) Fish as models for the neuroendocrine regulation of reproduction and growth. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 119: 345–364.M. BlazquezPT BosmaEJ FraserKJ Van LookVL Trudeau1998Fish as models for the neuroendocrine regulation of reproduction and growth.Comp Biochem Physiol C Pharmacol Toxicol Endocrinol119345364
  6. 6. Koger CS, Teh SJ, Hinton DE (1999) Variations of light and temperature regimes and resulting effects on reproductive parameters in medaka (Oryzias latipes). Biol Reprod 61: 1287–1293.CS KogerSJ TehDE Hinton1999Variations of light and temperature regimes and resulting effects on reproductive parameters in medaka (Oryzias latipes).Biol Reprod6112871293
  7. 7. Ruan G, Allen GC, Yamazaki S, McMahon DG (2008) An Autonomous Circadian Clock in the Inner Mouse Retina Regulated by Dopamine and GABA. PLOS Biology 6: 2248–2265.G. RuanGC AllenS. YamazakiDG McMahon2008An Autonomous Circadian Clock in the Inner Mouse Retina Regulated by Dopamine and GABA.PLOS Biology622482265
  8. 8. Trudeau VL (1997) Neuroendocrine regulation of gonadotrophin II release and gonadal growth in the goldfish, Carassius auratus. Rev Reprod 2: 55–68.VL Trudeau1997Neuroendocrine regulation of gonadotrophin II release and gonadal growth in the goldfish, Carassius auratus.Rev Reprod25568
  9. 9. Thiery JC, Chemineau P, Hernandez X, Migaud M, Malpaux B (2002) Neuroendocrine interactions and seasonality. Domest Anim Endocrinol 23: 87–100.JC ThieryP. ChemineauX. HernandezM. MigaudB. Malpaux2002Neuroendocrine interactions and seasonality.Domest Anim Endocrinol2387100
  10. 10. Lincoln GA, Richardson M (1998) Photo-neuroendocrine control of seasonal cycles in body weight, pelage growth and reproduction: lessons from the HPD sheep model. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 119: 283–294.GA LincolnM. Richardson1998Photo-neuroendocrine control of seasonal cycles in body weight, pelage growth and reproduction: lessons from the HPD sheep model.Comp Biochem Physiol C Pharmacol Toxicol Endocrinol119283294
  11. 11. Callard GV, Kruger A, Betka M (1995) The goldfish as a model for studying neuroestrogen synthesis, localization, and action in the brain and visual system. Environ Health Perspect 103: Suppl 751–57.GV CallardA. KrugerM. Betka1995The goldfish as a model for studying neuroestrogen synthesis, localization, and action in the brain and visual system.Environ Health Perspect103Suppl 75157
  12. 12. Popesku JT, Martyniuk CJ, Mennigen J, Xiong H, Zhang D, et al. (2008) The goldfish (Carassius auratus) as a model for neuroendocrine signaling. Mol Cell Endocrinol 293: 43–56.JT PopeskuCJ MartyniukJ. MennigenH. XiongD. Zhang2008The goldfish (Carassius auratus) as a model for neuroendocrine signaling.Mol Cell Endocrinol2934356
  13. 13. Peter RE, Chang JP, Nahorniak CS, Omeljaniuk RJ, Sokolowska M, et al. (1986) Interactions of catecholamines and GnRH in regulation of gonadotropin secretion in teleost fish. Recent Prog Horm Res 42: 513–548.RE PeterJP ChangCS NahorniakRJ OmeljaniukM. Sokolowska1986Interactions of catecholamines and GnRH in regulation of gonadotropin secretion in teleost fish.Recent Prog Horm Res42513548
  14. 14. Davies B, Hannah LT, Randall CF, Bromage N, Williams LM (1994) Central melatonin binding sites in rainbow trout (Onchorhynchus mykiss). Gen Comp Endocrinol 96: 19–26.B. DaviesLT HannahCF RandallN. BromageLM Williams1994Central melatonin binding sites in rainbow trout (Onchorhynchus mykiss).Gen Comp Endocrinol961926
  15. 15. Barrell GK, Thrun LA, Brown ME, Viguie C, Karsch FJ (2000) Importance of photoperiodic signal quality to entrainment of the circannual reproductive rhythm of the ewe. Biol Reprod 63: 769–774.GK BarrellLA ThrunME BrownC. ViguieFJ Karsch2000Importance of photoperiodic signal quality to entrainment of the circannual reproductive rhythm of the ewe.Biol Reprod63769774
  16. 16. Darrow JM, Goldman BD (1985) Circadian regulation of pineal melatonin and reproduction in the Djungarian hamster. J Biol Rhythms 1: 39–54.JM DarrowBD Goldman1985Circadian regulation of pineal melatonin and reproduction in the Djungarian hamster.J Biol Rhythms13954
  17. 17. Ekstrom P, Vanecek J (1992) Localization of 2-[125I]iodomelatonin binding sites in the brain of the Atlantic salmon, Salmo salar L. Neuroendocrinology 55: 529–537.P. EkstromJ. Vanecek1992Localization of 2-[125I]iodomelatonin binding sites in the brain of the Atlantic salmon, Salmo salar L.Neuroendocrinology55529537
  18. 18. Mazurais D, Brierley I, Anglade I, Drew J, Randall C, et al. (1999) Central melatonin receptors in the rainbow trout: comparative distribution of ligand binding and gene expression. J Comp Neurol 409: 313–324.D. MazuraisI. BrierleyI. AngladeJ. DrewC. Randall1999Central melatonin receptors in the rainbow trout: comparative distribution of ligand binding and gene expression.J Comp Neurol409313324
  19. 19. Vernadakis AJ, Bemis WE, Bittman EL (1998) Localization and partial characterization of melatonin receptors in amphioxus, hagfish, lamprey, and skate. Gen Comp Endocrinol 110: 67–78.AJ VernadakisWE BemisEL Bittman1998Localization and partial characterization of melatonin receptors in amphioxus, hagfish, lamprey, and skate.Gen Comp Endocrinol1106778
  20. 20. Somoza GM, Miranda LA, Strobl-Mazzulla P, Guilgur LG (2002) Gonadotropin-releasing hormone (GnRH): from fish to mammalian brains. Cell Mol Neurobiol 22: 589–609.GM SomozaLA MirandaP. Strobl-MazzullaLG Guilgur2002Gonadotropin-releasing hormone (GnRH): from fish to mammalian brains.Cell Mol Neurobiol22589609
  21. 21. Sorensen PW, Christensen TA, Stacey NE (1998) Discrimination of pheromonal cues in fish: emerging parallels with insects. Curr Opin Neurobiol 8: 458–467.PW SorensenTA ChristensenNE Stacey1998Discrimination of pheromonal cues in fish: emerging parallels with insects.Curr Opin Neurobiol8458467
  22. 22. Trudeau VL, Spanswick D, Fraser EJ, Lariviere K, Crump D, et al. (2000) The role of amino acid neurotransmitters in the regulation of pituitary gonadotropin release in fish. Biochem Cell Biol 78: 241–259.VL TrudeauD. SpanswickEJ FraserK. LariviereD. Crump2000The role of amino acid neurotransmitters in the regulation of pituitary gonadotropin release in fish.Biochem Cell Biol78241259
  23. 23. Pasmanik M, Callard GV (1988) Changes in brain aromatase and 5 alpha-reductase activities correlate significantly with seasonal reproductive cycles in goldfish (Carassius auratus). Endocrinology 122: 1349–1356.M. PasmanikGV Callard1988Changes in brain aromatase and 5 alpha-reductase activities correlate significantly with seasonal reproductive cycles in goldfish (Carassius auratus).Endocrinology12213491356
  24. 24. Trudeau VL, Peter RE, Sloley BD (1991) Testosterone and estradiol potentiate the serum gonadotropin response to gonadotropin-releasing hormone in goldfish. Biol Reprod 44: 951–960.VL TrudeauRE PeterBD Sloley1991Testosterone and estradiol potentiate the serum gonadotropin response to gonadotropin-releasing hormone in goldfish.Biol Reprod44951960
  25. 25. Gonzalez A, Piferrer F (2003) Aromatase activity in the European sea bass (Dicentrarchus labrax L.) brain. Distribution and changes in relation to age, sex, and the annual reproductive cycle. Gen Comp Endocrinol 132: 223–230.A. GonzalezF. Piferrer2003Aromatase activity in the European sea bass (Dicentrarchus labrax L.) brain. Distribution and changes in relation to age, sex, and the annual reproductive cycle.Gen Comp Endocrinol132223230
  26. 26. Joy KP, Tharakan B, Goos HJ (1999) Distribution of gamma-aminobutyric acid in catfish (Heteropneustes fossilis) forebrain in relation to season, ovariectomy and E2 replacement, and effects of GABA administration on plasma gonadotropin-II level. Comp Biochem Physiol A Mol Integr Physiol 123: 369–376.KP JoyB. TharakanHJ Goos1999Distribution of gamma-aminobutyric acid in catfish (Heteropneustes fossilis) forebrain in relation to season, ovariectomy and E2 replacement, and effects of GABA administration on plasma gonadotropin-II level.Comp Biochem Physiol A Mol Integr Physiol123369376
  27. 27. McNulty JA (1982) Morphologic evidence for seasonal changes in the pineal organ of the goldfish, Carassius auratus; a quantitative study. Reprod Nutr Dev 22: 1061–1072.JA McNulty1982Morphologic evidence for seasonal changes in the pineal organ of the goldfish, Carassius auratus; a quantitative study.Reprod Nutr Dev2210611072
  28. 28. Munkittrick KR, Leatherland JF (1983) Seasonal changes in the pituitary- gonad axis of feral goldfish, Carassius auratus L., from Ontario, Canada. Journal of Fish Biology 24: 75–90.KR MunkittrickJF Leatherland1983Seasonal changes in the pituitary- gonad axis of feral goldfish, Carassius auratus L., from Ontario, Canada.Journal of Fish Biology247590
  29. 29. Gelinas D, Pitoc GA, Callard GV (1998) Isolation of a goldfish brain cytochrome P450 aromatase cDNA: mRNA expression during the seasonal cycle and after steroid treatment. Mol Cell Endocrinol 138: 81–93.D. GelinasGA PitocGV Callard1998Isolation of a goldfish brain cytochrome P450 aromatase cDNA: mRNA expression during the seasonal cycle and after steroid treatment.Mol Cell Endocrinol1388193
  30. 30. Jodo A, Kitahashi T, Taniyama S, Bhandari RK, Ueda H, et al. (2005) Seasonal variation in the expression of five subtypes of gonadotropin-releasing hormone receptor genes in the brain of masu salmon from immaturity to spawning. Zoolog Sci 22: 1331–1338.A. JodoT. KitahashiS. TaniyamaRK BhandariH. Ueda2005Seasonal variation in the expression of five subtypes of gonadotropin-releasing hormone receptor genes in the brain of masu salmon from immaturity to spawning.Zoolog Sci2213311338
  31. 31. Lariviere K, Samia M, Lister A, Van Der Kraak G, Trudeau VL (2005) Sex steroid regulation of brain glutamic acid decarboxylase (GAD) mRNA is season-dependent and sexually dimorphic in the goldfish Carassius auratus. Brain Res Mol Brain Res 141: 1–9.K. LariviereM. SamiaA. ListerG. Van Der KraakVL Trudeau2005Sex steroid regulation of brain glutamic acid decarboxylase (GAD) mRNA is season-dependent and sexually dimorphic in the goldfish Carassius auratus.Brain Res Mol Brain Res14119
  32. 32. Peyon P, Saied H, Lin X, Peter RE (1999) Postprandial, seasonal and sexual variations in cholecystokinin gene expression in goldfish brain. Brain Res Mol Brain Res 74: 190–196.P. PeyonH. SaiedX. LinRE Peter1999Postprandial, seasonal and sexual variations in cholecystokinin gene expression in goldfish brain.Brain Res Mol Brain Res74190196
  33. 33. Peyon P, Saied H, Lin X, Peter RE (2000) Preprotachykinin gene expression in goldfish brain: sexual, seasonal, and postprandial variations. Peptides 21: 225–231.P. PeyonH. SaiedX. LinRE Peter2000Preprotachykinin gene expression in goldfish brain: sexual, seasonal, and postprandial variations.Peptides21225231
  34. 34. Samia M, Lariviere KE, Rochon MH, Hibbert BM, Basak A, et al. (2004) Seasonal cyclicity of secretogranin-II expression and its modulation by sex steroids and GnRH in the female goldfish pituitary. Gen Comp Endocrinol 139: 198–205.M. SamiaKE LariviereMH RochonBM HibbertA. Basak2004Seasonal cyclicity of secretogranin-II expression and its modulation by sex steroids and GnRH in the female goldfish pituitary.Gen Comp Endocrinol139198205
  35. 35. Sohn YC, Yoshiura Y, Kobayashi M, Aida K (1999) Seasonal changes in mRNA levels of gonadotropin and thyrotropin subunits in the goldfish, Carassius auratus. Gen Comp Endocrinol 113: 436–444.YC SohnY. YoshiuraM. KobayashiK. Aida1999Seasonal changes in mRNA levels of gonadotropin and thyrotropin subunits in the goldfish, Carassius auratus.Gen Comp Endocrinol113436444
  36. 36. Orlando EF, Binczik GA, Denslow ND, Guillette LJ Jr (2007) Reproductive seasonality of the female Florida gar, Lepisosteus platyrhincus. Gen Comp Endocrinol 151: 318–324.EF OrlandoGA BinczikND DenslowLJ Guillette Jr2007Reproductive seasonality of the female Florida gar, Lepisosteus platyrhincus.Gen Comp Endocrinol151318324
  37. 37. Martyniuk CJ, Xiong H, Crump K, Chiu S, Sardana R, et al. (2006) Gene expression profiling in the neuroendocrine brain of male goldfish (Carassius auratus) exposed to 17alpha-ethinylestradiol. Physiol Genomics 27: 328–336.CJ MartyniukH. XiongK. CrumpS. ChiuR. Sardana2006Gene expression profiling in the neuroendocrine brain of male goldfish (Carassius auratus) exposed to 17alpha-ethinylestradiol.Physiol Genomics27328336
  38. 38. Marlatt VL, Martyniuk CJ, Zhang D, Xiong H, Watt J, et al. (2008) Auto-regulation of estrogen receptor subtypes and gene expression profiling of 17beta-estradiol action in the neuroendocrine axis of male goldfish. Mol Cell Endocrinol 283: 38–48.VL MarlattCJ MartyniukD. ZhangH. XiongJ. Watt2008Auto-regulation of estrogen receptor subtypes and gene expression profiling of 17beta-estradiol action in the neuroendocrine axis of male goldfish.Mol Cell Endocrinol2833848
  39. 39. Mennigen JA, Martyniuk CJ, Crump K, Xiong H, Zhao E, et al. (2008) Effects of fluoxetine on the reproductive axis of female goldfish (Carassius auratus). Physiol Genomics 35: 273–282.JA MennigenCJ MartyniukK. CrumpH. XiongE. Zhao2008Effects of fluoxetine on the reproductive axis of female goldfish (Carassius auratus).Physiol Genomics35273282
  40. 40. Williams DR, Li W, Hughes MA, Gonzalez SF, Vernon C, et al. (2008) Genomic resources and microarrays for the common carp Cyprinus carpio L. Journal of Fish Biology 72: 2095–2117.DR WilliamsW. LiMA HughesSF GonzalezC. Vernon2008Genomic resources and microarrays for the common carp Cyprinus carpio L.Journal of Fish Biology7220952117
  41. 41. Basilevsky A (1994) Statistical Factor Analysis and Related Methods: Theory and Applications. A. Basilevsky1994Statistical Factor Analysis and Related Methods: Theory and Applications
  42. 42. Pearson K (1901) On lines and planes of closest fit to systems of points in space. Philosophical Magazine 2: 449–572.K. Pearson1901On lines and planes of closest fit to systems of points in space.Philosophical Magazine2449572
  43. 43. Sherlock G (2001) Analysis of large-scale gene expression data. Brief Bioinform 2: 350–362.G. Sherlock2001Analysis of large-scale gene expression data.Brief Bioinform2350362
  44. 44. Storey JD, Dai JY, Leek JT (2007) The optimal discovery procedure for large-scale significance testing, with applications to comparative microarray experiments. Biostatistics 8: 414–432.JD StoreyJY DaiJT Leek2007The optimal discovery procedure for large-scale significance testing, with applications to comparative microarray experiments.Biostatistics8414432
  45. 45. Leek JT, Monsen E, Dabney AR, Storey JD (2006) EDGE: extraction and analysis of differential gene expression. Bioinformatics 22: 507–508.JT LeekE. MonsenAR DabneyJD Storey2006EDGE: extraction and analysis of differential gene expression.Bioinformatics22507508
  46. 46. Duston J, Bromage N (1987) Constant photoperiod regimes and the entrainment of the annual cycle of reproduction in the female rainbow trout (Salmo gairdneri). Gen Comp Endocrinol 65: 373–384.J. DustonN. Bromage1987Constant photoperiod regimes and the entrainment of the annual cycle of reproduction in the female rainbow trout (Salmo gairdneri).Gen Comp Endocrinol65373384
  47. 47. Razani H, Hanyu I, Aida K (1987) Critical daylength and temperature level for photoperiodism in gonadal maturation of goldfish. Exp Biol 47: 89–94.H. RazaniI. HanyuK. Aida1987Critical daylength and temperature level for photoperiodism in gonadal maturation of goldfish.Exp Biol478994
  48. 48. Hontela A, Peter RE (1983) Characteristics and functional significance of daily cycles in serum gonadotropin hormone levels in the goldfish. J Exp Zool 228: 543–550.A. HontelaRE Peter1983Characteristics and functional significance of daily cycles in serum gonadotropin hormone levels in the goldfish.J Exp Zool228543550
  49. 49. Bromage NR, Whitehead C, Breton B (1982) Relationships between serum levels of gonadotropin, oestradiol-17 beta, and vitellogenin in the control of ovarian development in the rainbow trout. II. The effects of alterations in environmental photoperiod. Gen Comp Endocrinol 47: 366–376.NR BromageC. WhiteheadB. Breton1982Relationships between serum levels of gonadotropin, oestradiol-17 beta, and vitellogenin in the control of ovarian development in the rainbow trout. II. The effects of alterations in environmental photoperiod.Gen Comp Endocrinol47366376
  50. 50. Dupre SM, Burt DW, Talbot R, Downing A, Mouzaki D, et al. (2008) Identification of melatonin-regulated genes in the ovine pituitary pars tuberalis, a target site for seasonal hormone control. Endocrinology 149: 5527–5539.SM DupreDW BurtR. TalbotA. DowningD. Mouzaki2008Identification of melatonin-regulated genes in the ovine pituitary pars tuberalis, a target site for seasonal hormone control.Endocrinology14955275539
  51. 51. Prendergast BJ, Mosinger B Jr, Kolattukudy PE, Nelson RJ (2002) Hypothalamic gene expression in reproductively photoresponsive and photorefractory Siberian hamsters. Proc Natl Acad Sci U S A 99: 16291–16296.BJ PrendergastB. Mosinger JrPE KolattukudyRJ Nelson2002Hypothalamic gene expression in reproductively photoresponsive and photorefractory Siberian hamsters.Proc Natl Acad Sci U S A991629116296
  52. 52. Trudeau VL, Sloley BD, Peter RE (1993) GABA stimulation of gonadotropin-II release in goldfish: involvement of GABAA receptors, dopamine, and sex steroids. Am J Physiol 265: R348–355.VL TrudeauBD SloleyRE Peter1993GABA stimulation of gonadotropin-II release in goldfish: involvement of GABAA receptors, dopamine, and sex steroids.Am J Physiol265R348355
  53. 53. Goodson JL, Bass AH (2000) Vasotocin innervation and modulation of vocal-acoustic circuitry in the teleost Porichthys notatus. J Comp Neurol 422: 363–379.JL GoodsonAH Bass2000Vasotocin innervation and modulation of vocal-acoustic circuitry in the teleost Porichthys notatus.J Comp Neurol422363379
  54. 54. Thompson RR, Walton JC (2004) Peptide effects on social behavior: effects of vasotocin and isotocin on social approach behavior in male goldfish (Carassius auratus). Behav Neurosci 118: 620–626.RR ThompsonJC Walton2004Peptide effects on social behavior: effects of vasotocin and isotocin on social approach behavior in male goldfish (Carassius auratus).Behav Neurosci118620626
  55. 55. Evans JJ (1996) Oxytocin and the control of LH. J Endocrinol 151: 169–174.JJ Evans1996Oxytocin and the control of LH.J Endocrinol151169174
  56. 56. Rettori V, Canteros G, Renoso R, Gimeno M, McCann SM (1997) Oxytocin stimulates the release of luteinizing hormone-releasing hormone from medial basal hypothalamic explants by releasing nitric oxide. Proc Natl Acad Sci U S A 94: 2741–2744.V. RettoriG. CanterosR. RenosoM. GimenoSM McCann1997Oxytocin stimulates the release of luteinizing hormone-releasing hormone from medial basal hypothalamic explants by releasing nitric oxide.Proc Natl Acad Sci U S A9427412744
  57. 57. Sigel E (2002) Mapping of the benzodiazepine recognition site on GABA(A) receptors. Curr Top Med Chem 2: 833–839.E. Sigel2002Mapping of the benzodiazepine recognition site on GABA(A) receptors.Curr Top Med Chem2833839
  58. 58. Zhang J, Suneja SK, Potashner SJ (2004) Protein kinase A and calcium/calmodulin-dependent protein kinase II regulate glycine and GABA release in auditory brain stem nuclei. J Neurosci Res 75: 361–370.J. ZhangSK SunejaSJ Potashner2004Protein kinase A and calcium/calmodulin-dependent protein kinase II regulate glycine and GABA release in auditory brain stem nuclei.J Neurosci Res75361370
  59. 59. Li L, Li Z, Sacks DB (2003) Calmodulin regulates the transcriptional activity of estrogen receptors. Selective inhibition of calmodulin function in subcellular compartments. J Biol Chem 278: 1195–1200.L. LiZ. LiDB Sacks2003Calmodulin regulates the transcriptional activity of estrogen receptors. Selective inhibition of calmodulin function in subcellular compartments.J Biol Chem27811951200
  60. 60. Shashoua VE (1991) Ependymin, a brain extracellular glycoprotein, and CNS plasticity. Ann N Y Acad Sci 627: 94–114.VE Shashoua1991Ependymin, a brain extracellular glycoprotein, and CNS plasticity.Ann N Y Acad Sci62794114
  61. 61. Forlano PM, Deitcher DL, Myers DA, Bass AH (2001) Anatomical distribution and cellular basis for high levels of aromatase activity in the brain of teleost fish: aromatase enzyme and mRNA expression identify glia as source. J Neurosci 21: 8943–8955.PM ForlanoDL DeitcherDA MyersAH Bass2001Anatomical distribution and cellular basis for high levels of aromatase activity in the brain of teleost fish: aromatase enzyme and mRNA expression identify glia as source.J Neurosci2189438955
  62. 62. Williams DR, Epperson LE, Li W, Hughes MA, Taylor R, et al. (2005) Seasonally hibernating phenotype assessed through transcript screening. Physiol Genomics 24: 13–22.DR WilliamsLE EppersonW. LiMA HughesR. Taylor2005Seasonally hibernating phenotype assessed through transcript screening.Physiol Genomics241322
  63. 63. Jones S, Pfister-Genskow M, Cirelli C, Benca RM (2008) Changes in brain gene expression during migration in the white-crowned sparrow. Brain Res Bull 76: 536–544.S. JonesM. Pfister-GenskowC. CirelliRM Benca2008Changes in brain gene expression during migration in the white-crowned sparrow.Brain Res Bull76536544
  64. 64. Westring CG, Ando H, Kitahashi T, Bhandari RK, Ueda H, et al. (2008) Seasonal changes in CRF-I and urotensin I transcript levels in masu salmon: correlation with cortisol secretion during spawning. Gen Comp Endocrinol 155: 126–140.CG WestringH. AndoT. KitahashiRK BhandariH. Ueda2008Seasonal changes in CRF-I and urotensin I transcript levels in masu salmon: correlation with cortisol secretion during spawning.Gen Comp Endocrinol155126140
  65. 65. Okuzawa K, Gen K, Bruysters M, Bogerd J, Gothilf Y, et al. (2003) Seasonal variation of the three native gonadotropin-releasing hormone messenger ribonucleic acids levels in the brain of female red seabream. Gen Comp Endocrinol 130: 324–332.K. OkuzawaK. GenM. BruystersJ. BogerdY. Gothilf2003Seasonal variation of the three native gonadotropin-releasing hormone messenger ribonucleic acids levels in the brain of female red seabream.Gen Comp Endocrinol130324332
  66. 66. Andersson E, Fjelldal PG, Klenke U, Vikingstad E, Taranger GL, et al. (2001) Three forms of GnRH in the brain and pituitary of the turbot, Scophthalmus maximus: immunological characterization and seasonal variation. Comp Biochem Physiol B Biochem Mol Biol 129: 551–558.E. AnderssonPG FjelldalU. KlenkeE. VikingstadGL Taranger2001Three forms of GnRH in the brain and pituitary of the turbot, Scophthalmus maximus: immunological characterization and seasonal variation.Comp Biochem Physiol B Biochem Mol Biol129551558
  67. 67. Forlano PM, Bass AH (2005) Seasonal plasticity of brain aromatase mRNA expression in glia: divergence across sex and vocal phenotypes. J Neurobiol 65: 37–49.PM ForlanoAH Bass2005Seasonal plasticity of brain aromatase mRNA expression in glia: divergence across sex and vocal phenotypes.J Neurobiol653749
  68. 68. Li GL, Liu XC, Lin HR (2007) Seasonal changes of serum sex steroids concentration and aromatase activity of gonad and brain in red-spotted grouper (Epinephelus akaara). Anim Reprod Sci 99: 156–166.GL LiXC LiuHR Lin2007Seasonal changes of serum sex steroids concentration and aromatase activity of gonad and brain in red-spotted grouper (Epinephelus akaara).Anim Reprod Sci99156166
  69. 69. Yang YH, Dudoit S, Luu P, Lin DM, Peng V, et al. (2002) Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation. Nucleic Acids Res 30: e15.YH YangS. DudoitP. LuuDM LinV. Peng2002Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation.Nucleic Acids Res30e15
  70. 70. Bolstad BM, Irizarry RA, Astrand M, Speed TP (2003) A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 19: 185–193.BM BolstadRA IrizarryM. AstrandTP Speed2003A comparison of normalization methods for high density oligonucleotide array data based on variance and bias.Bioinformatics19185193
  71. 71. Heyer LJ, Kruglyak S, Yooseph S (1999) Exploring expression data: identification and analysis of coexpressed genes. Genome Res 9: 1106–1115.LJ HeyerS. KruglyakS. Yooseph1999Exploring expression data: identification and analysis of coexpressed genes.Genome Res911061115
  72. 72. Saeed AI, Sharov V, White J, Li J, Liang W, et al. (2003) TM4: a free, open-source system for microarray data management and analysis. Biotechniques 34: 374–378.AI SaeedV. SharovJ. WhiteJ. LiW. Liang2003TM4: a free, open-source system for microarray data management and analysis.Biotechniques34374378
  73. 73. Conesa A, Gotz S, Garcia-Gomez JM, Terol J, Talon M, et al. (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21: 3674–3676.A. ConesaS. GotzJM Garcia-GomezJ. TerolM. Talon2005Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research.Bioinformatics2136743676
  74. 74. Bluthgen N, Brand K, Cajavec B, Swat M, Herzel H, et al. (2005) Biological profiling of gene groups utilizing Gene Ontology. Genome Inform 16: 106–115.N. BluthgenK. BrandB. CajavecM. SwatH. Herzel2005Biological profiling of gene groups utilizing Gene Ontology.Genome Inform16106115
  75. 75. Fleige S, Pfaffl MW (2006) RNA integrity and the effect on the real-time qRT-PCR performance. Mol Aspects Med 27: 126–139.S. FleigeMW Pfaffl2006RNA integrity and the effect on the real-time qRT-PCR performance.Mol Aspects Med27126139
  76. 76. You FM, Huo N, Gu YQ, Luo MC, Ma Y, et al. (2008) BatchPrimer3: a high throughput web application for PCR and sequencing primer design. BMC Bioinformatics 9: 253.FM YouN. HuoYQ GuMC LuoY. Ma2008BatchPrimer3: a high throughput web application for PCR and sequencing primer design.BMC Bioinformatics9253