Human activities have increased atmospheric concentrations of carbon dioxide by 36% during the past 200 years. One third of all anthropogenic CO2 has been absorbed by the oceans, reducing pH by about 0.1 of a unit and significantly altering their carbonate chemistry. There is widespread concern that these changes are altering marine habitats severely, but little or no attention has been given to the biota of estuarine and coastal settings, ecosystems that are less pH buffered because of naturally reduced alkalinity.
To address CO2-induced changes to estuarine calcification, veliger larvae of two oyster species, the Eastern oyster (Crassostrea virginica), and the Suminoe oyster (Crassostrea ariakensis) were grown in estuarine water under four pCO2 regimes, 280, 380, 560 and 800 µatm, to simulate atmospheric conditions in the pre-industrial era, present, and projected future concentrations in 50 and 100 years respectively. CO2 manipulations were made using an automated negative feedback control system that allowed continuous and precise control over the pCO2 in experimental aquaria. Larval growth was measured using image analysis, and calcification was measured by chemical analysis of calcium in their shells. C. virginica experienced a 16% decrease in shell area and a 42% reduction in calcium content when pre-industrial and end of 21st century pCO2 treatments were compared. C. ariakensis showed no change to either growth or calcification. Both species demonstrated net calcification and growth, even when aragonite was undersaturated, a result that runs counter to previous expectations for invertebrate larvae that produce aragonite shells.
Conclusions and Significance
Our results suggest that temperate estuarine and coastal ecosystems are vulnerable to the expected changes in water chemistry due to elevated atmospheric CO2 and that biological responses to acidification, especially calcifying biota, will be species-specific and therefore much more variable and complex than reported previously.
Citation: Miller AW, Reynolds AC, Sobrino C, Riedel GF (2009) Shellfish Face Uncertain Future in High CO2 World: Influence of Acidification on Oyster Larvae Calcification and Growth in Estuaries. PLoS ONE 4(5): e5661. https://doi.org/10.1371/journal.pone.0005661
Editor: Zoe Finkel, Mt. Alison University, Canada
Received: February 4, 2009; Accepted: April 27, 2009; Published: May 27, 2009
Copyright: © 2009 Miller et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: Funding for this research was provided by the Seward Johnson Endowment provided through the Smithsonian Marine Science Network as well as federal appropriations made to the Smithsonian Institution.
Competing interests: The authors have declared that no competing interests exist.
During the past 200 years the combustion of fossil fuels, deforestation, and land development have increased atmospheric concentrations of carbon dioxide by 36% and the rate of CO2 emission is expected to increase during the coming century –. Approximately one third of all anthropogenic CO2 has been absorbed by Earth's oceans –. Although the ocean has partially absorbed recently liberated anthropogenic atmospheric CO2, this has come at the expense of significantly reduced pH (acidification) and altered carbonate chemistry in the ocean's surface waters. Since ca. 1760, the pH of the ocean's surface (the upper 200 m of the water column) has decreased by approximately 0.1 of a unit, and further reductions of 0.1 to 0.5 units are expected during the next 100 years –. There is widespread concern that these changes will produce irreversible ecological regime shifts in marine habitats, such as massive reductions in coral reef habitats and their associated biodiversity as well as reduced availability of carbonate ions for calcifying biota –.
To date most research has focused on CO2-induced acidification in fully marine waters, while little attention has been devoted to lower salinity estuaries and temperate nearshore ecosystems. But coastal and estuarine biomes are among the most biologically productive and maintain some of the most extensive and measurable ecosystem services (e.g., commercial and recreational fisheries, fish and invertebrate nursery grounds, water purification, flood and storm surge protection, human recreation). Because they are shallower, less saline, and have lower alkalinity , estuaries and coastal marine habitats are more susceptible to changes in pH than the open ocean. Estuaries are also susceptible to substantial enrichment in CO2, produced by the respiration of both natural and anthropogenic carbon. While many estuaries already have high and variable pCO2 , atmospheric CO2 enrichment will shift the baseline toward even higher values. For these reasons, these ecosystems will likely experience more acute impacts from elevated CO2 in coming decades.
A direct consequence of CO2-induced acidification is the reduction of carbonate ion concentration  in the water column. Of special concern in marine and estuarine systems is the effect of rising CO2 on the saturation state of water with respect to calcium carbonate (Ω), whereand K′sp is the apparent solubility product –:Ω is therefore a proxy for the ease with which organisms can produce calcium carbonate (CaCO3). The water's saturation states with respect to aragonite and calcite, the two most commonly biomineralized forms of CaCO3, are diminished with increased partial pressures of CO2 (pCO2) .
In estuaries, Ω naturally decreases with decreasing salinity due to gradients in pH, Ca2+ and produced by the dilution of seawater with river water (see Cai and Wang ). A recent study by Salisbury et al.  indicates that seasonal discharges of acidic riverine water will further exacerbate acidification in estuaries, suggesting the possibility of negative impacts to shell fisheries.
In Chesapeake Bay, Wong  showed that water from the James River and ocean mix conservatively and that salinity and total alkalinity (TA) are linearly related from approximately 5 psu to 32 psu. At ∼18 psu, TA in the Bay is ∼1250 µmol/kg-SW. If conservative mixing of water and equilibrium with current atmospheric CO2 (380 µatm) are assumed, the aragonite compensation point (i.e., Ωarag = 1.0) at summer temperatures should lie near the 18 psu isopleth. As atmospheric CO2 increases, assuming Ca2+ and alkalinity remain at present levels, the aragonite compensation point in estuaries will shift seaward toward higher salinities. We contend that the aragonite compensation point has already shifted significantly toward the Bay's mouth since the beginning of the industrial era (Fig. 1). The compensation point for calcite, which is approximately 1.5 times less soluble than aragonite  occurs at lower salinities and will shift seaward in a similar way. In reality, estuaries are frequently not in equilibrium with the atmosphere, however, a change in atmospheric pCO2 will shift the baseline equilibrium to which estuaries tend, and result in wider spread elevated pCO2.
Theoretical aragonite compensation positions were plotted under past, present and predicted atmospheric CO2. Aragonite saturation was calculated using the program CO2SYS.XLS from alkalinity and atmospheric pCO2 at 25°C and 0 mbar pressure, assuming conservative mixing of Atlantic and the Susquehanna River alkalinities, and mean summer salinities (1985–2006 – data from Chesapeake Bay Program ). Aragonite is supersaturated seaward of compensation lines.
We expect bivalve larvae to be more vulnerable than adults to increased CO2 because larvae biomineralize aragonite, the more soluble form of CaCO3, rather than calcite, the predominant material used in adult shell. Weiss et al.  further indicate that during biomineralization some bivalve larvae, (e.g., Crassostrea gigas and Mercenaria mercenaria) generate an even more soluble amorphous CaCO3 as an ephemeral precursor to crystalline aragonite, and because larvae are generally less robust than adults to a variety of stresses. If larvae are indeed more susceptible to acidification, it may lead to their reduced performance, or even failure, ultimately leading to negative effects on oysters and other shellfish populations. Kurihara et al. observed at very high pCO2 (2268 µatm) that embryonic development and shell formation in Crassostrea gigas was inhibited during the initial 48 h following fertilization . How shifting carbonate chemistry will alter the ecological structure and function in benthic communities remains a critical gap in our knowledge –.
We conducted experiments on the veliger larvae of two closely related euryhaline oyster species, the Eastern oyster (Crassostrea virginica [Gmelin 1791]) and the Suminoe oyster (Crassostrea ariakensis [Fujita 1913]), under conditions of continuously controlled pH/pCO2 for up to 28 days. C. virginica is native to the Western Atlantic, but the recent landings are just 1% of their historical maximum in Chesapeake Bay, due in large measure to overfishing and disease –. The introduction of C. ariakensis to Chesapeake Bay has been considered in the hopes of restoring the oyster fishery and important ecosystem functions, such as water filtration and reduction of nutrients and sediment in the water column . We hypothesize that (a) oyster larvae will exhibit reduced growth and calcification at elevated pCO2 and (b) larval development will be adversely and measurably affected when Ωarag<1.0. By extension, oyster fisheries, other shellfish, and calcifying benthic fauna are expected to be negatively influenced by acidification.
Materials and Methods
All oyster larvae tested were obtained from the Virginia Institute for Marine Science's Eastern Shore Laboratory, Wachapreague, VA, where C. virginica and C. ariakensis were spawned in quarantined facilities. Experiment Ca 1 larvae were derived from C. ariakensis stocks originating in the Ariake Sea of Japan but accidentally transferred to the west coast of the US with shipments of C. gigas in the 1970s . Cv 1 were derived from Wachapreague seaside C. virginica stocks. Table 1 summarizes all experimental parameters for experiments Cv 1 and Ca 1. At 72 hours post fertilization, D-stage larvae were transferred from Wachapreague to a quarantined laboratory at the Smithsonian Environmental Research Center in Edgewater, MD, and placed into culture. To test the effects of elevated pCO2, reduced pH, and aragonite saturation state on larval growth and calcification, we cultured oyster larvae under controlled conditions that simulated a typical summer estuarine environment in upper mesohaline reaches of the Chesapeake Bay: salinity = 18 psu, temperature = 25°C, day/night cycle = 14:10 hrs. Experimental treatments were blocked across two incubators (Percival I-36 Controlled Environment Chambers with Philips 700 series 32 W fluorescent bulbs) and individual aquaria randomly positioned within each incubator. Incubator A contained 280 µatm and 380 µatm pCO2 treatments and incubator B contained 560 µatm and 800 µatm for all experiments. Prior to experiments, the PAR irradiance was measured inside each incubator and shown to be similar (A = 167.7±5.3 µE m−2 s−1 compared with B = 169.2±7.3 µE m−2 s−1 (mean±SEM).
Natural sea water was collected from Sinepuxent Bay, MD (∼28 psu) and Delaware Bay, DE (∼24 psu) and filtered through a 0.2 µm filter and diluted with deionized water to 18 psu. An open cell, two-point titration  determined the TA of the water just prior to the onset of each experiment. Total alkalinity and pCO2 targets for pre-industrial (pCO2 = 280 µatm), present day (pCO2 = 380 µatm), year 2050 (pCO2 = 560 µatm) and year 2100 (pCO2 = 800 µatm) settings, as projected by the IS92a “business as usual” scenario  were then entered into the computer program CO2SYS.XLS . The program was parameterized specifically with the two dissociation constants K1 and K2 for carbonic acid in estuarine waters of Cai and Wang  to determine corresponding experimental pH levels.
Larvae were grown in 4-L polycarbonate aquaria, under four pCO2 treatments, and each treatment included three replicate aquaria that were independently controlled for pCO2/pH (n = 12). Filtered water samples (0.2 µm) were collected from each aquarium every few days throughout the experiments for total dissolved inorganic carbon (TDIC) analysis. (To maintain water quality, water was changed in all aquaria every two days. TDIC samples were taken 24–48 hours after a water change.) Water samples were capped without head space and analyzed immediately, or stored at 4°C and analyzed within 48 hrs. TDIC was measured using a total organic carbon analyzer, outfitted with a phosphoric acid inorganic carbon reagent reaction chamber and non-dispersive infrared detector (Schimadzu TOC-V with IC reactor kit). TDIC was then partitioned into pCO2, bicarbonate, and carbonate and TA calculated with CO2SYS.XLS  (Table 1.) Experiments were ended when larvae of the largest size classes achieved competence as indicated by the presence of eye spots.
Experiments Cv 1 (Crassostrea virginica) and Ca 1 (C. ariakensis) were inoculated with 15,000 four day-old D-stage larvae respectively, and were grown for up to 28 days. Aquaria received controlled quantities of the microalgae Isochrysis galbana (Prymnesiophyceae) as food. Microalgae were grown in semi-continuous cultures using f/2-Guillard medium. Equal quantities of microalgae were pipetted daily from cultures of known density into each aquarium, with daily doses increasing as larvae grew larger. Target inoculation densities in each aquarium increased from 1.5·104cells·ml−1 at the beginning of an experiment to 1.0·105cells·ml−1 during the finals days, when larvae were larger and ingesting more food.
The pCO2 in each aquarium was sustained by intermittent bubbling with CO2-enriched air (commercially available, certified 1.0% CO2) and continuous aeration with CO2-stripped air. Soda-lime filters were used to strip CO2. Continuous aeration with CO2-stripped air oxygenated and mixed the water, while simultaneously driving CO2 out of the water and pushing pH upward. Each aquarium was continuously monitored with a pH probe (NBS scale) and control system. Once a target pH set point was exceeded, an automated controller opened a solenoid and delivered the 1% CO2 air mixture to an aquarium, thereby driving pH downward. The flow of CO2 was automatically stopped when the pH set point was reached. Based on hourly pH measurements, the negative feedback control system was shown to maintain pH (and pCO2) at near constant levels for up to 4 weeks. The four pH set points used corresponded to desired target pCO2 treatments for water of known total alkalinity. Probes were calibrated prior to the experiment using NIST traceable buffers (pH = 4.00 and 7.00), recalibrated at least one time during the experiment, and again at the conclusion of the experiment to ensure proper operation.
At the end of each experiment, larvae from each aquarium were collected and fixed in 95% ethanol. Random samples were taken from each replicate and photographed at 20× magnification; (n = 205±6.4 shells per replicate (mean±SEM)). Larval shells were positioned on their sides and photographed in silhouette. Outer shell areas were measured using digital image analysis  (Scion Image, ver. 22.214.171.124). All areas are reported as total outer surface areas (i.e., 2× photographed area = total surface area of both valves). The same samples were rinsed with deionized water to remove sea salts and ethanol, and the shells dissolved in trace metal grade HCl, and diluted to a known volume. Inductively coupled plasma/optical emission spectroscopy (ICP/OES) was used to measure the mean calcium content per shell from each replicate sample across treatments.
For each experimental treatment, the mean mass of CaCO3 per unit area (µg/µm2) was determined by dividing the mean CaCO3 content of shells by the mean shell area. These values were then divided by the density of crystalline aragonite (2.93 g/cm3)  to estimate the mean apparent aragonite thickness of larval shells. However, this uniformly overestimates shell thickness slightly, because image analysis calculated shell area in silhouette, thereby underestimating the true three-dimensional shell surface. This bias should not affect comparisons with treatments for the same organism.
Larval development proceeded from 96 h post fertilization to the eyed veliger and pediveliger stages (i.e., achieved competence) among the largest size classes in all treatments and across all experiments. Experiments Cv 1 and Ca 1 ran to larval ages of 30 d and 32 d, respectively.
Crassostrea virginica grew more slowly at elevated pCO2 than at either 280 or 380 µatm treatments, as indicated by analysis of variance (Cv 1: F3,8 = 6.605, n = 12, P = 0.015, Fig. 2a). No significant differences in shell growth were observed across pCO2 treatments for C. ariakensis (Ca 1: F3,8 = 0.024, n = 12, P = 0.995, Fig. 2b). Cumulative shell size frequencies for each species and treatment reveal: (1) that ninety percent of C. virginica shells exceeded the median size class of C. ariakensis and (2) clear treatment effects in C. virginica growth but none in C. ariakensis (Fig. 3).
Mean shell areas±SEM (µm2) (panels A and B) and mean shell CaCO3 content±SEM (µg/shell) (panels C and D) reported by pCO2 treatment±SEM (µatm) for two oyster species. Corresponding aragonite saturation states (Ωarag) are indicated for each treatment. Statistical differences determined by ANOVA and Tukey HSD tests. C. virginica grew more quickly than C. ariakensis under all treatments but experienced reduced growth at high pCO2. The growth of C. ariakensis was not noticeably affected by elevated pCO2 or aragonite saturation. C. virginica calcified less at elevated pCO2 and Ωarag<1.0 (panel C), whereas calcification of C. ariakensis was not significantly influenced by elevated pCO2 or aragonite saturation (panel D).
Black symbols and curves on right show results from Crassostrea virginica, experiment Cv 1, age = 30 d. Open symbols and left curves plot C. ariakensis results (experiment Ca 1, age = 32 d). PCO2 treatments = 280, 380, 560, 800 µatm. Number of shells per treatment = 615±26.7 (mean±SEM). C. virginica grew to markedly larger sizes than C. ariakensis. Ninety percent of C. virginica shells were as large as or larger than the median C. ariakensis size class (dashed line). Growth of C. virginica was influenced by pCO2 treatment and C. ariakensis was unaffected.
The amount of CaCO3 biomineralized by Crassostrea virginica larvae decreased as pCO2 increased (Fig. 2c). Analysis of variance confirmed significant differences among pCO2 treatments (Cv 1: F3,8 = 11.8026, n = 12, P = 0.0026). By contrast, no clear trends or differences in biomineralization were apparent for C. ariakensis (Ca 1: F3,7 = 0.6451, n = 11, P = 0.6103, Fig. 2d).
In each experiment, aragonite conditions ranged from supersaturating (Ωarag = 1.2−1.3) to undersaturating (Ωarag = 0.6). C. ariakensis was unaffected by differences in aragonite saturation, but growth and calcification in C. virginica were significantly curtailed when pCO2 was high and Ωarag<1.0 (Fig. 2). Importantly, both species achieved net growth even when the saturation state of aragonite was well below 1.0.
Although cross sectional measurements of larval shells were not performed directly, we derived an estimate of average shell thickness that is theoretically attributable to aragonite volume. For each experimental treatment, the mean CaCO3 content per shell was divided by the corresponding mean shell area (µg/µm2). This value was then divided by the density of crystalline aragonite (2.93×10−6µg/µm3), leaving the mean apparent aragonite thickness in µm. The mean apparent aragonite thickness was strikingly similar across treatments and experiments (ranging from 9 to 11 µm) regardless of species or larval age (Fig. 4).
Apparent aragonite thickness (µm) of larval shells = mean CaCO3 content (µg)/mean shell area (µm2)/aragonite density (2.93×10−6 µg/µm3). Aragonite thickness was similar across species and varied little across pCO2 treatments.
A second pair of similar experiments was conducted using a modified design (i.e., fed with a two algal species diet, use of a different strain of C. ariakensis, and a greater starting larval density). Because of ciliate contamination in one of the C. virginica treatments, the experiments had to be prematurely terminated. Nevertheless, at larval ages of 14 d, results were similar to those reported above, indicating significantly reduced shell area and calcium content for C. virginica at elevated pCO2, but no significant pCO2 effects for C. ariakensis.
Most ocean acidification studies have focused on either warm water corals or pelagic biota –, . In general, marine fauna exhibit reductions in calcification at elevated pCO2 (e.g., scleratinian corals, Gattuso et al. , Langdon ; pteropods, Feely et al. , Orr et al. ; foraminifera, Bijima et al. ; see Fabry et al.  for review of marine faunal responses to elevated pCO2). As a group, coccolithophores react more variably, appearing to show species-specific responses to elevated CO2 , with Iglesias-Rodriguez et al.  reporting increased calcification in Emiliania huxleyi at pCO2 at 750 µatm. Experiments to date have largely simulated elevated atmospheric CO2 in fully marine settings (i.e., high salinity), meaning that water was supersaturated for CaCO3 under all challenge treatments due the relatively high total alkalinity of the seawater.
In cases where marine mollusks have been subjected to aragonite undersaturation, the results have generally been deleterious. For example, Kurihara et al.  observed that calcification and shell formation was inhibited during early development of Crassostrea gigas larvae when seawater was undersaturated with respect to aragonite (Ωarag = 0.68). When adult pteropods (Clio pyramidata) were exposed to seawater that was undersaturated for aragonite, their shells began to dissolve within 48 hours , , . Green et al.  showed that newly set juvenile hard shell clams (Mercenaria mercenaria, <2.0 mm) grown in benthic sediments with porewater undersaturated for CaCO3 showed signs of corrosion and significantly increased mortality. Hall-Spencer et al.  have observed that the shells of adult gastropods living in ultra high pCO2 habitats associated with natural underwater volcanic CO2 vents in the Mediterranean also dissolve when Ω≪1.0.
In contrast, when C. virginica and C. ariakensis larvae were cultured continuously from 96 h post fertilization (D-stage) to ∼30 d in typical estuarine conditions (salinity = 18 psu, TA ≈1225 µmol/kg) and exposed to elevated pCO2 levels, both species appeared to grow, calcify and develop normally with no obvious morphological deformities, despite conditions of significant aragonite undersaturation (Ωarag = 0.6−0.7). These findings demonstrate the physiological capacity of oyster larvae to withstand prolonged exposure (up to 28 days) to high pCO2 and aragonite undersaturation, and run counter to expectations that aragonite shelled larvae should be especially prone to dissolution at high pCO2 . It should not be surprising that some mollusks can grow in undersaturated conditions; most fresh water mollusks are clearly well adapted to such conditions. Nevertheless, as atmospheric CO2 continues to rise, all calcifying organisms will be encountering conditions that they have not experienced in recent geologic history.
When Carriker and Palmer  studied the normal growth and development of C. virginica larval shells using scanning electron microscopy, they found uniform, extremely thin shells, 4–6 µm across a range of larvae 100–350 µm in length. The thinness of the shells for their area, ca. 2–4% of the total length, is likely an adaptation to planktonic living. In our study, both oyster species generated larval shells that were of similar mean thickness, regardless of pCO2, Ωarag, or shell area. We interpret the pattern of similar shell thickness as further evidence of normal larval shell development.
Regardless of their shared physiological tolerance to undersaturated aragonite, C. virginica and C. ariakensis have divergent responses to elevated pCO2. C. virginica demonstrates consistently reduced growth and calcification at high pCO2. Conversely, C. ariakensis larvae are not differentially affected by pCO2, but do grow and calcify more slowly than C. virginica under all pCO2 treatments. These differences suggest that CO2-induced acidification is species-specific and will have unpredictable consequences in estuaries and nearshore ecosystems, even in closely related species.
From strictly thermodynamic considerations, the process of calcification in undersaturated environments (Ωarag<1.0) must require the input of exogenous energy to go forward. Weiner and Dove  describe two predominant processes of biomineralization widely used by marine and estuarine invertebrates. Extracellular mineralization is used by mollusks, scleratinian corals, bryozoans and some foraminifera and requires active pumping of ions or secretion and active transport of vesicles containing aqueous solutions of high ionic concentrations across cell membranes to produce conditions favorable for calcification. Coccolithophores, echinoderms, and other formaminifera use intracellular biomineralization, whereby nucleation of mineral structures occurs directly inside vesicles using similar active transport systems. The resulting carbonate structures are then secreted across the cell membrane (Weiner and Dove  and references therein). As aragonite becomes less saturated, the energetic costs for biomineralization should become progressively more expensive.
We do not perceive aragonite undersaturation as a “formal” barrier to calcification by marine or estuarine organisms in general. Aragonite saturation state is rather a convenient index to the ease with which biomineralization can be carried out. The higher the saturation state, the greater the relative availability of both Ca2+ and , and the lower the energy requirement for biochemical pumping to generate local supersaturation used when producing shell material. Just as species differ according to many environmental requirements, they likely differ in their ability or efficiency to supply energy for calcification. Marine taxa such as corals and pteropods, which are well adapted to marine conditions where both Ca2+ and have been abundant and relatively constant for millions of years, may have problems calcifying as declines due to anthropogenic CO2 enrichment, even though their water remains above saturation for both aragonite and calcite. In contrast, larvae of the euryhaline oysters C. virginica and C. ariakensis, cultured in mesohaline conditions with lower Ca2+ and CO32+ are able to generate and maintain aragonite shells when aragonite is somewhat below saturation. Although calcification is suppressed in lower saturation states for C. virginica, C. ariakensis is less affected by these conditions. Estuaries and open oceans differ substantially in their physical and chemical characteristics and dynamics, as do the evolutionary histories of their biota. For these reasons, expectations about the biological response to increasing atmospheric CO2 and acidification ought to be different as well.
Because of extreme spatial and temporal heterogeneity in estuaries and coastal systems, uniform equilibrium with atmospheric CO2 is neither expected nor achieved –. Estuarine habitats where shellfish settle and live often experience localized high pCO2, up to as much as 6000 µatm, due to benthic respiration . Our results suggest that for one key species, C. virginica, growth and calcification are diminished at relatively low enhancements of pCO2, conditions that fall within the bounds of variation that currently exist in its native habitat. When combined with existing natural forcing, future increases to pCO2 may influence significantly the success of larvae, and ultimately the success and distribution of these oysters in Chesapeake Bay and similar systems.
We predict that as atmospheric CO2 concentrations increase, conditions in estuaries will be less favorable for calcification in all salinity zones and will pose new environmental stresses on their inhabitants. For some calcifying species, the added energetic burden of producing shell under less favorable conditions may restrict growth. Our results suggest that this will be the case for C. virginica, but not necessarily for C. ariakensis. As energy costs mount, calcifying biota may be faced with a trade-off, either to allocate more energy to calcification at the cost of growth and stored energy reserves, or to accept a lesser degree of calcification in exchange for greater growth and more stored energy.
When invertebrate larvae become physiologically stressed from chemical and physical factors encountered in their environment, they sometimes delay settlement and metamorphosis. Pechenik  lists temperature, salinity, nutrition, low dissolved oxygen, pollution, and ultraviolet radiation as possible stressors that may induce delayed metamorphosis, and further indicates that larvae are more sensitive than adults to such stressors (Pechenik  and references therein). High pCO2 and reduced CaCO3 saturation may produce similar effects. For planktotrophic larvae, even minor increases in energy expenditure for shell production may reduce energy reserves that are critical for larval growth and survival. Metamorphosis is energetically demanding and relies entirely on energy stores accumulated during the larval stage –. If larval growth and calcification are adversely affected by CO2-induced acidification, larvae may well be energetically disadvantaged as they attempt metamorphosis, thereby suffering reduced survivorship and fitness. Furthermore, Pechenik  indicates that latent larval effects (i.e., impacts to post-metamorphic growth and mortality due to larval experience) may be common and species-specific among marine organisms.
Our findings suggest that as atmospheric CO2 rises in coming decades, the larvae of C. virginica, and perhaps other estuarine species, will grow and calcify more slowly than today. Unless larvae can achieve settling/metamorphic competence more efficiently in the future, slowed growth and calcification will lead to prolonged times in the water column, a situation that can result in higher pre-settlement larval mortality. Mortality from predation and disease increases the longer the larvae remain in the water column –. To illustrate the impact of relatively small changes in water column residence and mortality on recruitment, Kennedy  modeled the theoretical fate of C. virginica larval offspring from a single female, using natural daily mortality data from a variety of investigators. An 89% reduction in successful recruitment was postulated when metamorphosis was delayed five days (from 20 d to 25 d) and daily mortality rate increased from 20% to 25%. If higher water column mortality rates are coupled with lower larval quality at settlement (i.e., larvae settling with fewer energy reserves), then rates of recruitment could be expected to decrease in the future.
Whether or not prolonged time in the plankton and increased energy expenditures during larval growth and development from elevated CO2 adversely affect later life stages and, ultimately, the population dynamics and ecology of C. virginica or other species is unknown. Clearly, the larvae of both Crassostrea species that we studied have the physiological capacity to grow and calcify in undersaturated conditions, but if and how increasing pCO2 at all points in their habitat will adversely affect survivorship in the plankton as well as metamorphic success and recruitments dynamics remains to be tested.
On the Pacific coast of the United States, commercial oyster hatcheries are experiencing alarming and widespread difficulties keeping Pacific oyster (C. gigas) larvae alive in culture, with two of the largest hatcheries reporting production rates down by as much as 80%. Moreover, there has been little or no “natural” recruitment for several years in areas where C. gigas have previously established naturalized populations. (R. Downey, Pacific Coast Shellfish Growers Association, pers. comm.). In regions of upwelling along the continental shelf of western North America, Feely et al.  have determined that the pH and Ωarag of surface waters are more acidic and have less aragonite saturation than expected. At water depths of 40–120 m in many locations along the coast, but at 0 m in the region near the California/Oregon border, pH was reported to be ∼7.75 and Ωarag<1.0. Whether recent recruitment and aquaculture failures are linked to changes in carbonate chemistry are unknown, but should be investigated.
The natural spatial and temporal heterogeneity in salinity, alkalinity, pH, and pCO2 in estuaries impose more complex environmental stresses on calcifying biota than in the open ocean where the environment is far less variable. As atmospheric CO2 concentrations increase, the proportion of estuarine habitat undersaturated for aragonite will increase. In a high CO2 world, we predict that the aragonite compensation point in estuaries (salinity isopleth where Ωarag = 1.0) will shift seaward toward higher salinities. Because estuarine species have evolved in such variable environments, they may possess greater physiological capacity to respond to CO2-induced acidification than fully marine taxa. Nevertheless, adaptive physiological tolerance of larvae may not be sufficient to sustain populations of calcifying benthic species, including widespread economically important fisheries, in the face of changing atmospheric CO2.
We thank J.P. Megonigal, E. Jewett, P. Neale, and R. Osman for their consultation and technical advice. We thank S. Bonniwell and M. Luckenbach for supplying oyster larvae and culturing advice, as well as K. Erazmus, R. Lowenthal, and K. Stull, G. Riedel, D. Butera, S. Santagata, K. Klug, M. Hagan, and G.M. Ruiz for their contributions to these experiments and manuscript.
Conceived and designed the experiments: AWM CS GFR. Performed the experiments: AWM ACR GFR. Analyzed the data: AWM ACR CS GFR. Contributed reagents/materials/analysis tools: AWM ACR CS GFR. Wrote the paper: AWM ACR CS GFR.
- 1. Royal Society (2005) Ocean acidification due to increasing carbon dioxide. Policy document 12/05. The Royal Society. Royal Society2005Ocean acidification due to increasing carbon dioxide. Policy document 12/05The Royal Society60
- 2. Kleypas JA, Feely RA, Fabry VJ, Langdon C, Sabine CL, et al. (2006) 88.JA KleypasRA FeelyVJ FabryC. LangdonCL Sabine200688Impacts of Ocean Acidification on Coral Reefs and Other Marine Calcifiers: A guide for Future Research, report of a workshop held 18–20 April 2005, St. Petersburg, FL, sponsored by NSF, NOAA, and the U.S. Geological Survey. Impacts of Ocean Acidification on Coral Reefs and Other Marine Calcifiers: A guide for Future Research, report of a workshop held 18–20 April 2005, St. Petersburg, FL, sponsored by NSF, NOAA, and the U.S. Geological Survey.
- 3. Turley C, Blackford J, Widdicombe S, Lowe D, Nightingale PD, et al. (2006) Reviewing the impact of increased atmospheric CO2 on oceanic pH and the marine ecosystem. Schellnhuber HJ, Cramer W, Nakicenovic N, Wigley T, Yohe G, editors. Avoiding Dangerous Climate Change, Cambridge University Press, 8. pp. 65–70.C. TurleyJ. BlackfordS. WiddicombeD. LowePD Nightingale2006HJ SchellnhuberW. CramerN. NakicenovicT. WigleyG. YoheReviewing the impact of increased atmospheric CO2 on oceanic pH and the marine ecosystemAvoiding Dangerous Climate Change, Cambridge University Press, 86570
- 4. Sabine CL, Feely RA, Gruber N, Key RM, Lee K, et al. (2004) The Oceanic Sink for Anthropogenic CO2. Science 305: 367–371.CL SabineRA FeelyN. GruberRM KeyK. Lee2004The Oceanic Sink for Anthropogenic CO2.Science305367371
- 5. Canadell JG, Le Qué ré C, Raupach MR, Field CB, Buitenhuis ET, et al. (2007) Contributions to accelerating atmospheric CO2 growth from economic activity, carbon intensity, and efficiency of natural sinks. Proc Nat Acad Sci USA 104(47): 18866–18870.JG CanadellC. Le Qué réMR RaupachCB FieldET Buitenhuis2007Contributions to accelerating atmospheric CO2 growth from economic activity, carbon intensity, and efficiency of natural sinks.Proc Nat Acad Sci USA104(47)1886618870
- 6. Caldeira K, Wickett ME (2003) Anthropogenic carbon and ocean pH. Nature 425: 365.K. CaldeiraME Wickett2003Anthropogenic carbon and ocean pH.Nature425365
- 7. Caldeira K, Wickett ME (2005) Ocean model predictions of chemistry changes from carbon dioxide emissions to the atmosphere and ocean, J Geophys Res Oceans 110(C9), C09504: K. CaldeiraME Wickett2005Ocean model predictions of chemistry changes from carbon dioxide emissions to the atmosphere and ocean, J Geophys Res Oceans 110(C9),C09504
- 8. Orr JC, Fabry VJ, Aumont O, Bopp L, Doney SC, et al. (2005) Anthropogenic ocean acidification over the twenty-first century and its impact on calcifying organisms. Nature 437(7059): 681–686.JC OrrVJ FabryO. AumontL. BoppSC Doney2005Anthropogenic ocean acidification over the twenty-first century and its impact on calcifying organisms.Nature437(7059)681686
- 9. Langdon C (2002) Review of experimental evidence for effects of CO2 on calcification of reef builders. Proceedings of the 9th International Coral Reef Symposium 2: 1091–1098.C. Langdon2002Review of experimental evidence for effects of CO2 on calcification of reef builders.Proceedings of the 9th International Coral Reef Symposium210911098
- 10. Gazeau F, Quiblier C, Jansen JM, Gattuso J-P, Middelburg JJ, et al. (2007) Impact of elevated CO2 on shellfish calcification. Geophys Res Lett 34: L07603.F. GazeauC. QuiblierJM JansenJ-P GattusoJJ Middelburg2007Impact of elevated CO2 on shellfish calcification.Geophys Res Lett34L07603
- 11. Carpenter KE, Abrar M, Aeby G, Aronson RB, Banks S, et al. (2008) One-third of reef-building corals face elevated extinction risk from climate change and local impacts. Science 321: 560–563.KE CarpenterM. AbrarG. AebyRB AronsonS. Banks2008One-third of reef-building corals face elevated extinction risk from climate change and local impacts.Science321560563
- 12. Wong GT (1979) Alkalinity and pH in the southern Chesapeake Bay and the James River estuary. Limnol Oceanogr 24: 970–977.GT Wong1979Alkalinity and pH in the southern Chesapeake Bay and the James River estuary.Limnol Oceanogr24970977
- 13. Cai W-J, Wang Y (1998) The chemistry, fluxes, and sources of carbon dioxide in the estuarine waters of the Satilla and Althamaha Rivers, Georgia. Limnol Oceanogr 43: 657–668.W-J CaiY. Wang1998The chemistry, fluxes, and sources of carbon dioxide in the estuarine waters of the Satilla and Althamaha Rivers, Georgia.Limnol Oceanogr43657668
- 14. Doney SC, Fabry VJ, Feely RA, Kleypas JA (2009) Ocean acidification: the other CO2 problem. Annu Rev Mar Sci 1: 169–192.SC DoneyVJ FabryRA FeelyJA Kleypas2009Ocean acidification: the other CO2 problem.Annu Rev Mar Sci1169192
- 15. Feely RA, Sabine CL, Lee K, Berelson W, Kleypas J, et al. (2004) Impact of anthropogenic CO2 on the CaCO3 system in the oceans. Science 305: 362–366.RA FeelyCL SabineK. LeeW. BerelsonJ. Kleypas2004Impact of anthropogenic CO2 on the CaCO3 system in the oceans.Science305362366
- 16. Salisbury J, Green M, Hunt C, Campbell J (2008) Coastal acidification by rivers: A new threat to shellfish? EOS Trans AGU 89: 513.J. SalisburyM. GreenC. HuntJ. Campbell2008Coastal acidification by rivers: A new threat to shellfish?EOS Trans AGU89513
- 17. Weiss IM, Tuross N, Addadi L, Weiner S (2002) Mollusc larval shell formation: amorphous calcium carbonate is the precursor phase for aragonite. J Exp Biol 293: 478–491.IM WeissN. TurossL. AddadiS. Weiner2002Mollusc larval shell formation: amorphous calcium carbonate is the precursor phase for aragonite.J Exp Biol293478491
- 18. Kurihara H, Kato S, Ishimatsu A (2007) Effect of increased seawater pCO2 on early development of the oyster Crassostrea gigas. Aquat Biol 1: 91–98.H. KuriharaS. KatoA. Ishimatsu2007Effect of increased seawater pCO2 on early development of the oyster Crassostrea gigas.Aquat Biol19198
- 19. Kirby MX (2004) Fishing down the coast: Historical expansion and collapse of oyster fisheries along continental margins. Proc Acad Nat Sci U S A 101: 13096–13099.MX Kirby2004Fishing down the coast: Historical expansion and collapse of oyster fisheries along continental margins.Proc Acad Nat Sci U S A1011309613099
- 20. Breitburg DL, Riedel GF (2005) Multiple Stressors in Marine Systems. Marine Conservation Biology: The Science of Maintaining the Sea's Biodiversity (MCB). pp. 167–182.DL BreitburgGF Riedel2005Multiple Stressors in Marine Systems.Marine Conservation Biology: The Science of Maintaining the Sea's Biodiversity (MCB)167182In: EA Norse and LB Crowder editors.Island Press, Washington, D.C. Island Press, Washington, D.C.
- 21. OSB (2004) OSB2004Nonnative Oysters in Chesapeake Bay. Ocean Studies Board, Division on Earth and Life Studies, National Research Council of the National Academies, The National Academies Press, Washington, D.C., 325 p. Nonnative Oysters in Chesapeake Bay. Ocean Studies Board, Division on Earth and Life Studies, National Research Council of the National Academies, The National Academies Press, Washington, D.C., 325 p.
- 22. Breese WP, Malouf RE (1977) Hatchery rearing techniques for Crassostrea rivularis. Aquaculture 12: 123–126.WP BreeseRE Malouf1977Hatchery rearing techniques for Crassostrea rivularis.Aquaculture12123126
- 23. Edmond JM (1970) High precision determination of titration alkalinity and total carbon dioxide content of sea water by potentiometric titration. Deep Sea Res 17: 737–750.JM Edmond1970High precision determination of titration alkalinity and total carbon dioxide content of sea water by potentiometric titration.Deep Sea Res17737750
- 24. Leggett J, Pepper WJ, Swart RJ (1992) Emissions Scenarios for the IPCC: An Update. Climate Change 1992: The Supplementary Report to the IPCC Scientific Assessment. pp. 69–95.J. LeggettWJ PepperRJ Swart1992Emissions Scenarios for the IPCC: An Update.Climate Change 1992: The Supplementary Report to the IPCC Scientific Assessment6995In: J. T. Houghton, B. A. Callander, S. K. Varney editors.Cambridge University Press, Cambridge. Cambridge University Press, Cambridge.
- 25. Pelletier G, Lewis E, Wallace D (2007) CO2sys.xls: a calculator for the CO2 system in seawater for Microsoft Excel/VBA, Washington State Department of Ecology/Brookhaven National Laboratory, Olympia, WA/Upton, NY, USAG. PelletierE. LewisD. Wallace2007CO2sys.xls: a calculator for the CO2 system in seawater for Microsoft Excel/VBA, Washington State Department of Ecology/Brookhaven National Laboratory, Olympia, WA/Upton, NY, USA
- 26. Scion Image for Windows.Scion Image for Windows.Release alpha 126.96.36.199, © 2000–2001, Scion Corporation, www.scioncorp.com. Release alpha 188.8.131.52, © 2000–2001, Scion Corporation, www.scioncorp.com.
- 27. Lide DR (2007) DR Lide2007CRC Handbook of Chemistry and Physics, 88th Edition (Crc Handbook of Chemistry and Physics). CRC. CRC Handbook of Chemistry and Physics, 88th Edition (Crc Handbook of Chemistry and Physics). CRC.
- 28. Fabry VJ, Seibel BA, Feely RA, Orr JC (2008) Impacts of ocean acidification on marine fauna and ecosystem processes. ICES J Mar Sci 65: 414–432.VJ FabryBA SeibelRA FeelyJC Orr2008Impacts of ocean acidification on marine fauna and ecosystem processes.ICES J Mar Sci65414432
- 29. Gattuso JP, Frankignoulle M, Bourge I, Romaine S, Buddemeir RW (1998) Effect of calcium carbonate saturation of seawater on coral calcification. Global Planet Change 18: 37–46.JP GattusoM. FrankignoulleI. BourgeS. RomaineRW Buddemeir1998Effect of calcium carbonate saturation of seawater on coral calcification.Global Planet Change183746
- 30. Bijima J, Honisch B, Zeebe RE (2002) Impact of the ocean carbonate chemistry on living foraminifera shell weight: comment on “Carbonate ion concentration in glacial-age deepwaters of the Caribbean Sea” by WS Broecker and E Clark. Geochem. Geophys. Geosyst 3: 1064.J. BijimaB. HonischRE Zeebe2002Impact of the ocean carbonate chemistry on living foraminifera shell weight: comment on “Carbonate ion concentration in glacial-age deepwaters of the Caribbean Sea” by WS Broecker and E Clark. Geochem. Geophys.Geosyst31064
- 31. Fabry VJ (2008) Marine calcifiers in a high-CO2 ocean. Science 320: 120–122.VJ Fabry2008Marine calcifiers in a high-CO2 ocean.Science320120122
- 32. Iglesias-Rodriguez MD, Halloran PR, Rickaby REM, Hall IR, Colmenero-Hidalgo E, et al. (2008) Phytoplankton calcification in a high-CO2 world. Science 320: 336–340.MD Iglesias-RodriguezPR HalloranREM RickabyIR HallE. Colmenero-Hidalgo2008Phytoplankton calcification in a high-CO2 world.Science320336340
- 33. Green MA, Jones ME, Boudreau CL, Moore RL, Westman BA (2004) Dissolution mortality of juvenile bivalves in coastal marine deposits. Limnol Oceanogr 49(3): 727–734.MA GreenME JonesCL BoudreauRL MooreBA Westman2004Dissolution mortality of juvenile bivalves in coastal marine deposits.Limnol Oceanogr49(3)727734
- 34. Hall-Spencer JM, Rodolfo-Metalpa M, Martin S, Ransome E, Fine M, et al. (2008) Volcanic carbon dioxide vents show ecosystem effects of ocean acidification. Nature-online. JM Hall-SpencerM. Rodolfo-MetalpaS. MartinE. RansomeM. Fine2008Volcanic carbon dioxide vents show ecosystem effects of ocean acidification.Nature-online
- 35. Carriker MR, Palmer RE (1979) Ultrastructural morphogenesis of prodissoconch and early dissoconch valves of the oyster Crassostrea virginica. Proc Natl Shellfish. Assoc 69: 102–128.MR CarrikerRE Palmer1979Ultrastructural morphogenesis of prodissoconch and early dissoconch valves of the oyster Crassostrea virginica.Proc Natl Shellfish. Assoc69102128
- 36. Weiner S, Dove PM (2003) An overview of biomineralization processes and the problem of the vital effect. Biomineralization 1–29.S. WeinerPM Dove2003An overview of biomineralization processes and the problem of the vital effect.Biomineralization129In Dove PM, DeYoreo JJ, Weiner S, editors.Reviews in Mineralogy & Geochemistry 54. Reviews in Mineralogy & Geochemistry 54.
- 37. Smith SV, Hollibaugh JT (1997) Annual cycle and interannual variability of ecosystem metabolism in a temperate climate embayment. Ecol Monogr 67: 509–533.SV SmithJT Hollibaugh1997Annual cycle and interannual variability of ecosystem metabolism in a temperate climate embayment.Ecol Monogr67509533
- 38. Frankignoulle M, Abril G, Borges A, Bourge I, Canon C, et al. (1998) Carbon dioxide emission from European estuaries. Science 282: 434–436.M. FrankignoulleG. AbrilA. BorgesI. BourgeC. Canon1998Carbon dioxide emission from European estuaries.Science282434436
- 39. Cai W-J, Pomeroy LR, Moran MA, Wang Y (1999) Oxygen and carbon dioxide mass balance for the estuarine–intertidal marsh complex of five rivers in the southeastern U.S. Limno. Oceanogr 44: 639–649.W-J CaiLR PomeroyMA MoranY. Wang1999Oxygen and carbon dioxide mass balance for the estuarine–intertidal marsh complex of five rivers in the southeastern U.S.Limno. Oceanogr44639649
- 40. Pechenik JA (1999) On the advantages and disadvantages of larval stages in benthic marine invertebrate life cycles. Mar Ecol Prog Ser 177: 269–297.JA Pechenik1999On the advantages and disadvantages of larval stages in benthic marine invertebrate life cycles.Mar Ecol Prog Ser177269297
- 41. Pechenik JA (2006) Larval experience and latent effects—metamorphosis is not a new beginning. Integr Comp Biol 46: 323–333.JA Pechenik2006Larval experience and latent effects—metamorphosis is not a new beginning.Integr Comp Biol46323333
- 42. Burke K, Batller E, Miron G, Ouellette M, Tremblay R (2008) Larval quality of a nonnative bivalve species (European oyster, Ostrea edulis) off the east Canadian coast. J Shellfish Res 27(4): 701–710.K. BurkeE. BatllerG. MironM. OuelletteR. Tremblay2008Larval quality of a nonnative bivalve species (European oyster, Ostrea edulis) off the east Canadian coast.J Shellfish Res27(4)701710
- 43. Morgan SG (1996) Life and death in the plankton: laval mortality and adaptation. Ecology of Marine Invertebrate Larvae. pp. 279–321.SG Morgan1996Life and death in the plankton: laval mortality and adaptation.Ecology of Marine Invertebrate Larvae279321In: L. McEdward, editor.CRC Press, New York, NY. CRC Press, New York, NY.
- 44. Underwood AJ, Fairweather PG (1989) Supply-side ecology and benthic marine assemblages. Trends Ecol Evol 4: 16–20.AJ UnderwoodPG Fairweather1989Supply-side ecology and benthic marine assemblages.Trends Ecol Evol41620
- 45. Kennedy VS (1996) Biology of larvae and spat. The eastern oyster, Crassostrea virginica. pp. 371–441.VS Kennedy1996Biology of larvae and spat.The eastern oyster, Crassostrea virginica371441In: VS Kennedy, RIE Newell and AF Eble, editors.Maryland Sea Grant, College Park, MD. Maryland Sea Grant, College Park, MD.
- 46. Feely RA, Sabine CL, Hernandez-Ayon JM, Ianson D, Hales B (2008) Evidence for upwelling of corrosive acidified water onto the continental shelf. Science 320(5882): 1490–1492.RA FeelyCL SabineJM Hernandez-AyonD. IansonB. Hales2008Evidence for upwelling of corrosive acidified water onto the continental shelf.Science320(5882)14901492
- 47. Chesapeake Bay Program. Chesapeake Bay Program Water Quality Database 1984–present.Chesapeake Bay Program. Chesapeake Bay Program Water Quality Database 1984–present.Accessed October 20, 2008, from http://www.chesapeakebay.net/wquality.htm. Accessed October 20, 2008, from http://www.chesapeakebay.net/wquality.htm.