Betulinic Acid (BetA) and its derivatives have been extensively studied in the past for their anti-tumor effects, but relatively little is known about its precursor Betulin (BE). We found that BE induces apoptosis utilizing a similar mechanism as BetA and is prevented by cyclosporin A (CsA). BE induces cell death more rapidly as compared to BetA, but to achieve similar amounts of cell death a considerably higher concentration of BE is needed. Interestingly, we observed that cholesterol sensitized cells to BE-induced apoptosis, while there was no effect of cholesterol when combined with BetA. Despite the significantly enhanced cytotoxicity, the mode of cell death was not changed as CsA completely abrogated cell death. These results indicate that BE has potent anti-tumor activity especially in combination with cholesterol.
Citation: Mullauer FB, Kessler JH, Medema JP (2009) Betulin Is a Potent Anti-Tumor Agent that Is Enhanced by Cholesterol. PLoS ONE 4(4): e1. https://doi.org/10.1371/journal.pone.0005361
Editor: Mikhail V. Blagosklonny, Ordway Research Institute, United States of America
Received: February 12, 2009; Accepted: March 23, 2009; Published: April 28, 2009
Copyright: © 2009 Mullauer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The work is supported by the “stichting nationaal fonds tegen kanker” http://www.tegenkanker.nl/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Triterpenoids are extensively studied for the potential use as anticancer agents. One of the most promising compounds in this class is Betulinic Acid (BetA), but its effect is limited by the poor solubility of the compound. A lot of effort is therefore put into the development of derivatives of BetA with the goal to develop even more powerful compounds and to achieve better solubility for enhanced in vivo administration –. BetA has been modified at many different positions including C1-4, C-20, C-28 and A-, D- and E ring with different outcomes , . For example, Kvasanica et al found 3beta-O-phthalic esters from BetA more cytotoxic and polar in comparison to BetA itself . In contrast, generation of different C-28 ester derivatives did not result in enhanced cytotoxicity . On the other hand, C-28 amino acid conjugates made by Jeong et al showed improved selective toxicity and solubility  and a C-3 modified BetA derivative has shown promising results in a human colon cancer xenograft model .
BetA can be found in numerous different plants, but it can also be obtained by a simple 2 step reaction from its more abundantly available precursor molecule Betulin (BE) . BE is easily isolated and therefore plays an important role as raw material for the production of BetA and other biologically active compounds . BE itself has been shown in the past to only possess limited or no cytotoxic effects on cancer cells , . For example it was shown to be inactive against MEL-2 (melanoma) cells when compared to other BetA derivatives . Several other melanoma lines (G361, SK-MEL-28) leukemia lines (HL60, U937, K562), and neuroblastoma (GOTO, NB-1) cell lines were also found to be more resistant to BE than to other tested lupane triterpenes . In contrast, a recent report found BE to be active against colorectal (DLD-1), breast (MCF7), prostate ( PC-3) and lung (A549 ) cancer cell lines , and for A549 it was shown that apoptosis was induced . Apoptosis is one of the major cell death pathways induced by anti tumor agents. In principle, two main pathways can be distinguished, the extrinsic or death receptor pathway and the intrinsic or mitochondrial pathway with the latter being regulated by the Bcl-2 family of proteins . Numerous studies have shown that BetA induces apoptosis via the mitochondrial pathway –, however, to our knowledge, it is currently not clear how BE induces cell death. Here we show that apoptosis induction by BE does not involve the death receptor pathway, but is dependent on the mitochondria. Nevertheless, similar as we have previously shown for BetA , cytochrome c release and caspase activation occur independently of the Bcl-2 family proteins but are blocked in the presence of cyclosporin A (CsA), an inhibitor of the mitochondrial permeability transition (PT) pore. Furthermore we found that cholesterol strongly enhances the cytotoxic effects induced by BE but not BetA. Our results suggest that BE should not be regarded as an inactive precursor, but as a potent anti-tumor agent.
Materials and Methods
Betulin (≥98% pure; Sigma-Aldrich, St Louis, MO, USA) and Betulinic Acid (≥99% pure; BioSolutions Halle, Germany) were dissolved in DMSO at 4 mg/ml, cholesterol (Sigma-Aldrich) was dissolved at 5 mM in DMSO. Aliquots were kept frozen. Propidium iodide (PI), zVAD.fmk (benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone), etoposide and cyclosporin A were purchased from Sigma-Aldrich, Mitosox was obtained from Invitrogen (Carlsbad, CA, USA).
Anti-PARP (#9542; Cell Signaling Technology, Danvers, MA, USA) and anti-cytochrome c (clone 6H2.B4; BD Biosciences, San Diego, CA, USA) were used.
Cell lines: A549 and Hela were obtained from the ATCC, FADD-deficient, Caspase 8- deficient and control Jurkat cells (JA3) were kindly provided by Dr John Blenis (Harvard Medical School, Boston), Jurkat cells over-expressing Bcl-2 by Dr Jannie Borst (NKI, Amsterdam) and Bax/Bak double knockout (DKO) mouse embryonic fibroblasts (MEFs) and wild-type control MEFs were from Dr Stanley Korsmeyer.
Cell death analysis
Overall cell death was assessed as previously described  by PI exclusion assay. Briefly, cells were incubated with 1 µg/ml PI and measured by flow cytometry.
Cells were incubated in Nicoletti buffer containing 50 µg/ml PI for at least 24 hours before analysis via flow cytometry.
Western blot analysis (immunoblotting)
Cells were lysed using Triton X-100 buffer and for protein quantification a BCA kit from PIERCE was used. SDS-PAGE was performed and proteins were transferred onto a PVDF transfer membrane (Amersham Biosciences). Blocking of unspecific binding sites was achieved by incubation of the membrane in 5% low fat milk powder in PBS/0.2% Tween-20 (blocking buffer) for 1 hour at room temperature. Primary antibody incubation was performed overnight at 4°C and secondary antibody (HRP labeled) incubation for 2 hours at room temperature. For chemiluminescent detection ECL from Amersham Biosciences was used in combination with a LAS-3000 imaging system.
For ROS measurements the highly selective dye for mitochondrial superoxide Mitosox was used. Cells were incubated with 5 µM Mitosox in pre-warmed tissue culture medium at 37°C for 10 min before flow cytometry analysis.
Cytochrome c release by FACS staining
Cytochrome c release was measured as previously described by Waterhouse et al . First, outer cell membrane permeabilization was achieved by incubation for 5–10 minutes with 50 µg/ml digitonin in PBS containing 100 mM KCl. Cells were then fixed in 4% paraformaldehyde for 30 minutes at room temperature, washed and incubated in blocking buffer (3% BSA, 0.05% saponin, 0.02% azide in PBS supplemented with normal goat serum, dilution 1:200). Anti cytochrome c incubation was done overnight at 4°C and for flow cytometric detection a FITC conjugated secondary antibody was applied.
Cholesterol strongly enhances cytotoxic effects of BE but not BetA
Previously we have shown that BetA induces cell death in Jurkat T leukemia cells in a concentration and time-dependent fashion . Here we show that low concentrations (5 µg/ml) of BetA are non toxic up to 48 hours incubation and show limited cell death after 72 hours (Figure 1A). In contrast, when 7.5 µg/ml BetA or more is used almost all cells are PI positive after 48 to 72 hours (Figure 1A). To analyze whether Betulin (BE), the precursor of BetA, is capable of inducing cell death we titrated BE on Jurkat T Leukemia cells. In contrast to previous reports we show here that BE is capable of killing cells, but required higher concentrations than BetA. However, it appeared that cell death induced by BE is more efficient after 12 hours when compared to BetA and maximum cell death is achieved after 24 hours (Figure 1C).
Jurkat cells were treated with the indicated concentrations of BetA (A), BetA in combination with 5 µM cholesterol (B), BE (C), BE in combination with 5 µM cholesterol (D) or various concentrations of cholesterol only (E). Cell death was monitored after 12, 24, 48 and 72 hours using PI exclusion. A549 lung cancer (F) and HeLa cervix carcinoma (G) cell lines were treated with 5 µM cholesterol (chol), 5 µg/ml BE (5 BE) or the combination of 5 µg/ml BE with 5 µM cholesterol (5 BE+chol) and after 24 hours cell death was analyzed via PI exclusion.
We have found previously that when using the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay to measure BetA  or BE (unpublished data) induced cytotoxicity, results were much more pronounced when compared to other assays such as PI exclusion and clonogenic survival . This decrease in MTT conversion is likely the result of a direct effect of BetA on the mitochondria and was accompanied by a different morphological appearance of the formazan precipitates. While normal formazan formation shows a punctuate appearance, BetA and BE-induced formazan formation shows the rapid appearance of needle-like structures on the cell surface (Figure S1). Interestingly, cholesterol, which shares some structural similarities with BE and BetA, has been reported to have a comparable effect in the MTT assay – (Figure S1). This suggests that cholesterol, BetA and BE may share common targets in the cell. To clarify if this feature is related to the cytotoxicity of these compounds we decided to analyze the effect of cholesterol on cell death and combine cholesterol with either BetA or BE and measure PI exclusion after various time points. Cholesterol itself did not induce cell death in Jurkat cells (Figure 1E) and it did not enhance cytotoxicity of BetA at all time points measured (Figure 1B). However, the combination of BE with cholesterol resulted in massive cell death in Jurkat cells even when very small concentrations of BE were used (2.5 and 5 µg/ml BE, Figure 1D). To rule out that this is a cell type specific effect we analyzed cell death in A549 (lung carcinoma) and HeLa (cervical carcinoma) cells exposed to either BE or BE in combination with cholesterol. Similar to what was observed with the Jurkat cells, both solid cancer cell lines displayed massive cell death when treated with the combination of BE and cholesterol, whereas BE by itself showed only minor toxicity at the concentration used (Figure 1F and 1G).
BE/Cholesterol induces apoptosis in Jurkat cells
To identify the nature of cell death induced by BE/Cholesterol we investigated the apoptotic pathway. Apoptosis has been previously reported to be the cell death pathway induced by BE in A549 lung cancer cells . We assessed DNA fragmentation as an apoptosis read-out in Jurkat cells treated for 24 hours with either cholesterol, BE or the combination of both. In cells treated with cholesterol only, DNA fragmentation was completely absent (Figure 2A), consistent with the lack of cell death. BE, at 5 µg/ml, induced only moderate DNA fragmentation. However, when combined with cholesterol DNA was clearly fragmented (Figure 2A). To verify these results we performed immunoblotting for the classical caspase target PARP and observed similar effects: Upon BE treatment PARP was processed to some extent and this was strongly enhanced by addition of cholesterol (Figure 2B). Importantly, both, DNA fragmentation and PARP cleavage were blocked when cells were pre-treated with zVAD.fmk (a pan-caspase inhibitor) confirming that both are caspase-mediated events (Figure 2A and 2B).
(A) Jurkat cells were pretreated with 20 µM zVAD.fmk for at least one hour prior to addition of either DMSO, 5 µM cholesterol (5 Chol), 5 µg/ml BE (5 BE) or 5 µg/ml BE in combination with 5 µM cholesterol (5 BE+chol). After 24 hours DNA fragmentation was assessed by FACS analysis of propidium iodide (PI) stained nuclei. (B) Jurkat cells were treated as described in (A) but after 24 hours PARP cleavage was assessed by immunoblotting. The protein kinase ERK was used as a loading control.
The death receptor pathway is not involved in BE/cholesterol induced apoptosis
Cholesterol is an important constituent of cell membranes where it plays a crucial role in maintaining integrity and fluidity . In addition, cholesterol-enriched micro-domains, so called lipid rafts, are important signal transduction platforms , which have been related to apoptosis  and changes in plasma cholesterol levels have been associated with Fas-FADD complex formation and caspase-8 activation , . BetA has been shown to induce apoptosis independently of the extrinsic pathway . However, because of the strong apoptosis-enhancing effects of cholesterol when combined with BE, we decided to investigate the involvement of this pathway by applying BE/cholesterol on Jurkat cells either deficient for FADD or caspase-8. Recently we showed that the FADD and caspase-8 deficient cells were completely resistant to Fas-induced apoptosis . Here this resistance was further confirmed using TRAIL (Figure 3A). Despite the resistance towards the extrinsic pathway, neither cell line showed decreased DNA fragmentation when treated with BE/cholesterol (Figure 3B), indicating that the death receptor pathway is not involved in BE/cholesterol-induced apoptosis.
Jurkat control (JA3), FADD deficient (FADD ko) or caspase-8 deficient (Casp-8 ko) cells were treated with TRAIL (0.5 µg/ml plus 1 µg/ml anti-FLAG) (A) or with either 5 µM cholesterol (chol) or 5 µg/ml BE in combination with 5 µM cholesterol (5 BE+chol) and after 24 hours DNA fragmentation was analyzed.
BE/cholesterol induced apoptosis is mechanistically related to BetA induced apoptosis
BetA induced apoptosis has been clearly linked to the mitochondria – with the consistently described features of cytochrome c release and induction of reactive oxygen species (ROS) –. These events were initially described to be Bcl-2 family dependent , , however, our recent evidence suggests only a minor role for the Bcl-2 family proteins. Instead we proposed a direct effect on the PT-pore . To test if BE/cholesterol induces apoptosis via similar mechanisms as BetA we investigated the mitochondrial pathway of apoptosis.
BE/cholesterol showed clear cytochrome c release in Jurkat cells. Importantly, there was only a slight difference in cytochrome c release in the Bcl-2 over-expressing cells (Figure 4A), but this difference was statistically not significant (paired t-test). Jurkat cells over-expressing Bcl-2 were completely resistant to etoposide (Figure. 4A). In contrast to the lack of effect of Bcl-2 over-expression, CsA provided almost complete protection (Figure 4A). To determine if ROS are produced upon BE/cholesterol treatment we used a dye specifically detecting mitochondrial superoxide. Both wildtype as well as Bcl-2 over-expressing cells showed clear increase in ROS, strikingly this was again abolished in the presence of CsA (Figure 4B). To verify that these events resemble the amount of apoptosis and overall cell death we measured DNA fragmentation and PI exclusion respectively. Bcl-2 over-expression did not provide any protection whereas CsA effectively prevented both, apoptosis and cell death (Figure 4C and 4D). In order to find out if Bcl-2 over-expression causes a delay in apoptosis as is the case with BetA , we performed a kinetic analysis. Cell death and DNA fragmentation were measured after various time points from 0–24 hours. At all time points we did not observe any difference in sensitivity to BE/cholesterol, further underscoring the lack of inhibition by Bcl-2 (Figure 4E and 4F). These results suggest that BE/cholesterol kills Jurkat cells by inducing mitochondrial damage that leads to cytochrome c release and apoptosis which is completely independent of Bcl-2.
(A) Jurkat control (wt) or Bcl-2 over-expressing cells (Bcl-2) were treated as indicated (5BE = 5 µg/ml BE; chol = 5 µM cholesterol; CsA = 5 µg/ml cyclosporin A), after 24 hours intracellular staining for cytochrome c release was performed. (B, C, D) Jurkat control (wt) or Bcl-2 over-expressing cells were treated with 5 µg/ml BE/ 5 µM cholesterol either in the absence or presence of 5 µg/ml cyclosporin A. After 24 hours ROS (B), DNA fragmentation (C) and overall cell death (D) were assessed by FACS analysis. (E, F) Jurkat control (wt) or Bcl-2 over-expressing cells were treated with 5 µg/ml BE/ 5 µM cholesterol and PI exclusion (E) or DNA fragmentation (F) were measured after 0, 4, 8, 16 and 24 hours.
To further determine the efficacy of BE/cholesterol and to find out if Bax and Bak are involved in BE/cholesterol induced cytotoxicity we used Bax/Bak double-knockout (DKO) mouse embryonic fibroblasts (MEFs). DKO MEFs are resistant to drugs such as etoposide, staurosporine, UVC or actinomycin D, all targeting the Bcl-2 family regulated mitochondrial pathway . We measured PI exclusion and found DKO MEFs to be sensitive to BE/cholesterol, as a control for the functionality of the cells etoposide was included (Figure 5A). We assessed if apoptosis was induced like in BetA treated cells by analyzing PARP cleavage. PARP was clearly processed in wildtype as well as in DKO MEFs, suggesting that Bax and Bak are not essential in BE/cholesterol induced apoptosis (Figure 5B). Also cytochrome c release was not prevented in DKO MEFs (Figure 5C), further substantiating that Bax and Bak are not required for BE/cholesterol mediated cytotoxicity. Similar to Jurkat cells, CsA provided complete protection against cell death (Figure 5A), apoptosis (Figure 5B) and cytochrome c release (Figure 5C), confirming the crucial role for the mitochondrial permeability transition in BE/cholesterol induced cytotoxicity.
(A) Wildtype (wt) or Bax/Bak double knockout (DKO) mouse embryonic fibroblasts (MEFs) were treated as indicated and after 24 hours cell death was assessed by PI exclusion. Etoposide was included as a control for functionality of the cells. (B) Wt or DKO MEFs were treated as indicated and after 24 hours cells were subjected to immunoblotting to determine PARP processing. ERK was used as control for equal protein amounts. (C) Wt and DKO MEFs were treated as indicated for 24 hours before measuring cytochrome c release by intracellular FACS staining.
BE is a natural compound, which contains derivatives that have been shown to possess strong anti-tumor properties , . Here we provide evidence that BE itself, especially in combination with cholesterol (BE/cholesterol), is very potent in killing cancer cells in vitro (Figure 1). BE/cholesterol induces apoptosis in a similar manner as BetA and does not involve the extrinsic pathway of apoptosis (Figure 3), but instead apoptosis depends on the mitochondrial pathway (Figure 4). However, as we reported for BetA, this pathway is activated in an unconventional manner as cytochrome c release and apoptosis are induced in cells over-expressing Bcl-2 (Figure 4) or in cells deficient for Bax/Bak (Figure 5), while both events are blocked by CsA (Figure 4 and Figure 5). This indicates that permeability transition is pivotal in the process of BE/cholesterol induced cytotoxicity.
Despite the strong similarities, and the almost identical structure of BE and BetA, there are also important differences in comparison to BetA induced apoptosis. We previously showed that Bcl-2 over-expression delayed BetA-induced apoptosis, but curiously in the case of BE/cholesterol it has very limited effect on the amount of cytotoxicity induced (Figure 4). Furthermore, CsA by itself provides much stronger protection in the case of BE/cholesterol in Jurkat cells, while BetA treated Jurkat cells are only completely protected when a combination of CsA with Bcl-2 over-expression is used.
This difference between BetA and BE/cholesterol is even more remarkable when considering the time dependency of cytotoxicity of both molecules: For BetA the maximum effect requires around 48–72 hours and a dose of 7.5–10 µg/ml (Figure 1A and 1B), while BE/cholesterol induced death is already maximum at 24 hours. Nevertheless, CsA is capable of providing efficient protection.
Striking is the fact, that cholesterol strongly enhances the cytotoxic effects of BE but not BetA (Figure 1B and 1D) whilst being completely non-toxic on its own, even at very high concentrations (Figure 1E). Currently we do not know the mechanism by which cholesterol acts as a “cytotoxicity-amplifier” for BE but it likely involves membrane integrity. Cholesterol is abundantly present in the plasmamembrane and it is possible that changes in cholesterol content can affect the amount of BE that is taken up by a cell.
The effect on MTT conversion to formazan (MTT measures mitochondrial enzymatic activity , ) by all three compounds, BetA, BE and cholesterol, suggests a common target in the mitochondria. Even though this is clearly not directly related to cytotoxicity, as cholesterol on its own is completely non-toxic, it may point to a mechanism that sensitizes cells to BE. It is not clear how this is orchestrated but it could involve the mitochondrial membrane, for instance mitochondrial PT pore opening. The exact composition of the pore has yet to be established but adenine-nucleotide-translocator (ANT), voltage-dependent-anion-channel (VDAC) and cyclophilin D are discussed as core components in the currently accepted model . PT pore opening is influenced by the amount of cholesterol present in the mitochondrial membrane, cholesterol affects VDAC function  and impairs ANT mediated PT through altered membrane fluidity . So cholesterol-induced effects on the PT pore may facilitate BE-induced opening. Why this then does not influence BetA-induced opening is unclear at this point and will require further investigation. In this light it is also important to realize that Bcl-2 over-expression delays BetA-induced apoptosis , while CsA can only partially prevent the induction of apoptosis. This suggests that BetA may has a direct effect on the PT pore, which is blocked by CsA and maybe also induces a more classical Bcl-2-dependent pathway to cytochrome c release. This latter seems absent when using BE and may be the reason these compounds react slightly different to CsA and potentially also cholesterol.
To further evaluate the anti-tumor properties of BE/cholesterol in vivo studies will be required. Preliminary results from a pharmacokinetic study using triterpene extract (TE) mainly consisting of Betulin suggest that it is safe; no signs of toxicity were observed in rats or dogs in a subchronic toxicity study . Another study investigated the effects of BE on the central nervous system (CNS) with the conclusion that there was no effect of BE on muscle tone and coordination in mice; doses up to 100 mg/kg bodyweight were used . Interestingly another study explored the antinociceptive properties of Betulin in mice and results suggest that it is even more active than aspirin and paracetamol .
It will be interesting to explore the combined effects of BE and cholesterol in vivo. Because cholesterol is ubiquitously present in the body it is unlikely that additional applied cholesterol is useful for in vivo effects of BE as an anti-tumor agent. Our results indicate that the amount of cholesterol necessary (5 µM) for enhanced in vitro effects of BE are about 1000 times lower than normal plasma cholesterol levels in humans (5 mM). However the fast majority of this cholesterol is contained in LDL or HDL and it is therefore difficult to assess whether there is sufficient free cholesterol available to potentiate BE-induced apoptosis in vivo. Adding more cholesterol may not bear any significance though, but application of cholesterol containing Betulin-liposomes may be an interesting mode of applying this cytotoxic agent. In summary we conclude that Betulin by itself and in combination with cholesterol is a potent anti-cancer agent in vitro and warrants further investigation in vivo.
We would like to thank John Blenis, Jannie Borst, and Stanley Korsmeyer for kindly providing cells.
Conceived and designed the experiments: FM JHK JPM. Performed the experiments: FM JPM. Analyzed the data: FM JHK JPM. Wrote the paper: FM JPM.
- 1. Bi Y, Xu J, Wu X, Ye W, Yuan S, et al. (2007) Synthesis and cytotoxic activity of 17-carboxylic acid modified 23-hydroxy betulinic acid ester derivatives. Bioorg Med Chem Lett 17: 1475–1478.Y. BiJ. XuX. WuW. YeS. Yuan2007Synthesis and cytotoxic activity of 17-carboxylic acid modified 23-hydroxy betulinic acid ester derivatives.Bioorg Med Chem Lett1714751478
- 2. Rajendran P, Jaggi M, Singh MK, Mukherjee R, Burman AC (2008) Pharmacological evaluation of C-3 modified Betulinic acid derivatives with potent anticancer activity. Invest New Drugs 26: 25–34.P. RajendranM. JaggiMK SinghR. MukherjeeAC Burman2008Pharmacological evaluation of C-3 modified Betulinic acid derivatives with potent anticancer activity.Invest New Drugs262534
- 3. Cichewicz RH, Kouzi SA (2004) Chemistry, biological activity, and chemotherapeutic potential of betulinic acid for the prevention and treatment of cancer and HIV infection. Med Res Rev 24: 90–114.RH CichewiczSA Kouzi2004Chemistry, biological activity, and chemotherapeutic potential of betulinic acid for the prevention and treatment of cancer and HIV infection.Med Res Rev2490114
- 4. Mukherjee R, Kumar V, Srivastava SK, Agarwal SK, Burman AC (2006) Betulinic acid derivatives as anticancer agents: structure activity relationship. Anticancer Agents Med Chem 6: 271–279.R. MukherjeeV. KumarSK SrivastavaSK AgarwalAC Burman2006Betulinic acid derivatives as anticancer agents: structure activity relationship.Anticancer Agents Med Chem6271279
- 5. Kvasnica M, Sarek J, Klinotova E, Dzubak P, Hajduch M (2005) Synthesis of phthalates of betulinic acid and betulin with cytotoxic activity. Bioorg Med Chem 13: 3447–3454.M. KvasnicaJ. SarekE. KlinotovaP. DzubakM. Hajduch2005Synthesis of phthalates of betulinic acid and betulin with cytotoxic activity.Bioorg Med Chem1334473454
- 6. Jeong HJ, Chai HB, Park SY, Kim DS (1999) Preparation of amino acid conjugates of betulinic acid with activity against human melanoma. Bioorg Med Chem Lett 9: 1201–1204.HJ JeongHB ChaiSY ParkDS Kim1999Preparation of amino acid conjugates of betulinic acid with activity against human melanoma.Bioorg Med Chem Lett912011204
- 7. Alakurtti S, Makela T, Koskimies S, Yli-Kauhaluoma J (2006) Pharmacological properties of the ubiquitous natural product betulin. Eur J Pharm Sci 29: 1–13.S. AlakurttiT. MakelaS. KoskimiesJ. Yli-Kauhaluoma2006Pharmacological properties of the ubiquitous natural product betulin.Eur J Pharm Sci29113
- 8. Gao H, Wu L, Kuroyanagi M, Harada K, Kawahara N, et al. (2003) Antitumor-promoting constituents from Chaenomeles sinensis KOEHNE and their activities in JB6 mouse epidermal cells. Chem Pharm Bull (Tokyo) 51: 1318–1321.H. GaoL. WuM. KuroyanagiK. HaradaN. Kawahara2003Antitumor-promoting constituents from Chaenomeles sinensis KOEHNE and their activities in JB6 mouse epidermal cells.Chem Pharm Bull (Tokyo)5113181321
- 9. Kim DS, Pezzuto JM, Pisha E (1998) Synthesis of betulinic acid derivatives with activity against human melanoma. Bioorg Med Chem Lett 8: 1707–1712.DS KimJM PezzutoE. Pisha1998Synthesis of betulinic acid derivatives with activity against human melanoma.Bioorg Med Chem Lett817071712
- 10. Hata K, Hori K, Ogasawara H, Takahashi S (2003) Anti-leukemia activities of Lup-28-al-20(29)-en-3-one, a lupane triterpene. Toxicol Lett 143: 1–7.K. HataK. HoriH. OgasawaraS. Takahashi2003Anti-leukemia activities of Lup-28-al-20(29)-en-3-one, a lupane triterpene.Toxicol Lett14317
- 11. Gauthier C, Legault J, Lavoie S, Rondeau S, Tremblay S, et al. (2009) Synthesis and cytotoxicity of bidesmosidic betulin and betulinic acid saponins. J Nat Prod 72: 72–81.C. GauthierJ. LegaultS. LavoieS. RondeauS. Tremblay2009Synthesis and cytotoxicity of bidesmosidic betulin and betulinic acid saponins.J Nat Prod727281
- 12. Pyo JS, Roh SH, Kim DK, Lee JG, Lee YY, et al. (2009) Anti-Cancer Effect of Betulin on a Human Lung Cancer Cell Line: A Pharmacoproteomic Approach Using 2 D SDS PAGE Coupled with Nano-HPLC Tandem Mass Spectrometry. Planta Med 75: 127–131.JS PyoSH RohDK KimJG LeeYY Lee2009Anti-Cancer Effect of Betulin on a Human Lung Cancer Cell Line: A Pharmacoproteomic Approach Using 2 D SDS PAGE Coupled with Nano-HPLC Tandem Mass Spectrometry.Planta Med75127131
- 13. Jin Z, El-Deiry WS (2005) Overview of cell death signaling pathways. Cancer Biol Ther 4: 139–163.Z. JinWS El-Deiry2005Overview of cell death signaling pathways.Cancer Biol Ther4139163
- 14. Fulda S, Scaffidi C, Susin SA, Krammer PH, Kroemer G, et al. (1998) Activation of mitochondria and release of mitochondrial apoptogenic factors by betulinic acid. J Biol Chem 273: 33942–33948.S. FuldaC. ScaffidiSA SusinPH KrammerG. Kroemer1998Activation of mitochondria and release of mitochondrial apoptogenic factors by betulinic acid.J Biol Chem2733394233948
- 15. Fulda S, Susin SA, Kroemer G, Debatin KM (1998) Molecular ordering of apoptosis induced by anticancer drugs in neuroblastoma cells. Cancer Res 58: 4453–4460.S. FuldaSA SusinG. KroemerKM Debatin1998Molecular ordering of apoptosis induced by anticancer drugs in neuroblastoma cells.Cancer Res5844534460
- 16. Fulda S, Debatin KM (2000) Betulinic acid induces apoptosis through a direct effect on mitochondria in neuroectodermal tumors. Med Pediatr Oncol 35: 616–618.S. FuldaKM Debatin2000Betulinic acid induces apoptosis through a direct effect on mitochondria in neuroectodermal tumors.Med Pediatr Oncol35616618
- 17. Mullauer FB, Kessler JH, Medema JP (2009) Betulinic acid induces cytochrome c release and apoptosis in a Bax/Bak-independent, permeability transition pore dependent fashion. Apoptosis 14: 191–202.FB MullauerJH KesslerJP Medema2009Betulinic acid induces cytochrome c release and apoptosis in a Bax/Bak-independent, permeability transition pore dependent fashion.Apoptosis14191202
- 18. Kessler JH, Mullauer FB, de Roo GM, Medema JP (2007) Broad in vitro efficacy of plant-derived betulinic acid against cell lines derived from the most prevalent human cancer types. Cancer Lett 251: 132–145.JH KesslerFB MullauerGM de RooJP Medema2007Broad in vitro efficacy of plant-derived betulinic acid against cell lines derived from the most prevalent human cancer types.Cancer Lett251132145
- 19. Waterhouse NJ, Trapani JA (2003) A new quantitative assay for cytochrome c release in apoptotic cells. Cell Death Differ 10: 853–855.NJ WaterhouseJA Trapani2003A new quantitative assay for cytochrome c release in apoptotic cells.Cell Death Differ10853855
- 20. Abe K, Saito H (1998) Amyloid beta protein inhibits cellular MTT reduction not by suppression of mitochondrial succinate dehydrogenase but by acceleration of MTT formazan exocytosis in cultured rat cortical astrocytes. Neurosci Res 31: 295–305.K. AbeH. Saito1998Amyloid beta protein inhibits cellular MTT reduction not by suppression of mitochondrial succinate dehydrogenase but by acceleration of MTT formazan exocytosis in cultured rat cortical astrocytes.Neurosci Res31295305
- 21. Abe K, Saito H (1999) Cholesterol does not affect the toxicity of amyloid beta fragment but mimics its effect on MTT formazan exocytosis in cultured rat hippocampal neurons. Neurosci Res 35: 165–174.K. AbeH. Saito1999Cholesterol does not affect the toxicity of amyloid beta fragment but mimics its effect on MTT formazan exocytosis in cultured rat hippocampal neurons.Neurosci Res35165174
- 22. Ahmad S, Ahmad A, Schneider KB, White CW (2006) Cholesterol interferes with the MTT assay in human epithelial-like (A549) and endothelial (HLMVE and HCAE) cells. Int J Toxicol 25: 17–23.S. AhmadA. AhmadKB SchneiderCW White2006Cholesterol interferes with the MTT assay in human epithelial-like (A549) and endothelial (HLMVE and HCAE) cells.Int J Toxicol251723
- 23. Mouritsen OG, Zuckermann MJ (2004) What's so special about cholesterol? Lipids 39: 1101–1113.OG MouritsenMJ Zuckermann2004What's so special about cholesterol?Lipids3911011113
- 24. Helms JB, Zurzolo C (2004) Lipids as targeting signals: lipid rafts and intracellular trafficking. Traffic 5: 247–254.JB HelmsC. Zurzolo2004Lipids as targeting signals: lipid rafts and intracellular trafficking.Traffic5247254
- 25. Malorni W, Giammarioli AM, Garofalo T, Sorice M (2007) Dynamics of lipid raft components during lymphocyte apoptosis: the paradigmatic role of GD3. Apoptosis 12: 941–949.W. MalorniAM GiammarioliT. GarofaloM. Sorice2007Dynamics of lipid raft components during lymphocyte apoptosis: the paradigmatic role of GD3.Apoptosis12941949
- 26. Mollinedo F, Gajate C (2006) Fas/CD95 death receptor and lipid rafts: new targets for apoptosis-directed cancer therapy. Drug Resist Updat 9: 51–73.F. MollinedoC. Gajate2006Fas/CD95 death receptor and lipid rafts: new targets for apoptosis-directed cancer therapy.Drug Resist Updat95173
- 27. Gniadecki R (2004) Depletion of membrane cholesterol causes ligand-independent activation of Fas and apoptosis. Biochem Biophys Res Commun 320: 165–169.R. Gniadecki2004Depletion of membrane cholesterol causes ligand-independent activation of Fas and apoptosis.Biochem Biophys Res Commun320165169
- 28. Fulda S, Friesen C, Los M, Scaffidi C, Mier W, et al. (1997) Betulinic acid triggers CD95 (APO-1/Fas)- and p53-independent apoptosis via activation of caspases in neuroectodermal tumors. Cancer Res 57: 4956–4964.S. FuldaC. FriesenM. LosC. ScaffidiW. Mier1997Betulinic acid triggers CD95 (APO-1/Fas)- and p53-independent apoptosis via activation of caspases in neuroectodermal tumors.Cancer Res5749564964
- 29. Wick W, Grimmel C, Wagenknecht B, Dichgans J, Weller M (1999) Betulinic acid-induced apoptosis in glioma cells: A sequential requirement for new protein synthesis, formation of reactive oxygen species, and caspase processing. J Pharmacol Exp Ther 289: 1306–1312.W. WickC. GrimmelB. WagenknechtJ. DichgansM. Weller1999Betulinic acid-induced apoptosis in glioma cells: A sequential requirement for new protein synthesis, formation of reactive oxygen species, and caspase processing.J Pharmacol Exp Ther28913061312
- 30. Yamashita K, Lu H, Lu J, Chen G, Yokoyama T, et al. (2002) Effect of three triterpenoids, lupeol, betulin, and betulinic acid on the stimulus-induced superoxide generation and tyrosyl phosphorylation of proteins in human neutrophils. Clin Chim Acta 325: 91–96.K. YamashitaH. LuJ. LuG. ChenT. Yokoyama2002Effect of three triterpenoids, lupeol, betulin, and betulinic acid on the stimulus-induced superoxide generation and tyrosyl phosphorylation of proteins in human neutrophils.Clin Chim Acta3259196
- 31. Tan Y, Yu R, Pezzuto JM (2003) Betulinic acid-induced programmed cell death in human melanoma cells involves mitogen-activated protein kinase activation. Clin Cancer Res 9: 2866–2875.Y. TanR. YuJM Pezzuto2003Betulinic acid-induced programmed cell death in human melanoma cells involves mitogen-activated protein kinase activation.Clin Cancer Res928662875
- 32. Wei MC, Zong WX, Cheng EH, Lindsten T, Panoutsakopoulou V, et al. (2001) Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 292: 727–730.MC WeiWX ZongEH ChengT. LindstenV. Panoutsakopoulou2001Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death.Science292727730
- 33. Sarek J, Kvasnica M, Urban M, Klinot J, Hajduch M (2005) Correlation of cytotoxic activity of betulinines and their hydroxy analogues. Bioorg Med Chem Lett 15: 4196–4200.J. SarekM. KvasnicaM. UrbanJ. KlinotM. Hajduch2005Correlation of cytotoxic activity of betulinines and their hydroxy analogues.Bioorg Med Chem Lett1541964200
- 34. Slater TF, Sawyer B, Straeuli U (1963) Studies on Succinate-Tetrazolium Reductase Systems. III. Points of Coupling of Four Different Tetrazolium Salts. Biochim Biophys Acta 77: 383–393.TF SlaterB. SawyerU. Straeuli1963Studies on Succinate-Tetrazolium Reductase Systems. III. Points of Coupling of Four Different Tetrazolium Salts.Biochim Biophys Acta77383393
- 35. Grimm S, Brdiczka D (2007) The permeability transition pore in cell death. Apoptosis 12: 841–855.S. GrimmD. Brdiczka2007The permeability transition pore in cell death.Apoptosis12841855
- 36. Colell A, Garcia-Ruiz C, Lluis JM, Coll O, Mari M, et al. (2003) Cholesterol impairs the adenine nucleotide translocator-mediated mitochondrial permeability transition through altered membrane fluidity. J Biol Chem 278: 33928–33935.A. ColellC. Garcia-RuizJM LluisO. CollM. Mari2003Cholesterol impairs the adenine nucleotide translocator-mediated mitochondrial permeability transition through altered membrane fluidity.J Biol Chem2783392833935
- 37. Jager S, Laszczyk MN, Scheffler A (2008) A preliminary pharmacokinetic study of betulin, the main pentacyclic triterpene from extract of outer bark of birch (Betulae alba cortex). Molecules 13: 3224–3235.S. JagerMN LaszczykA. Scheffler2008A preliminary pharmacokinetic study of betulin, the main pentacyclic triterpene from extract of outer bark of birch (Betulae alba cortex).Molecules1332243235
- 38. Muceniece R, Saleniece K, Rumaks J, Krigere L, Dzirkale Z, et al. (2008) Betulin binds to gamma-aminobutyric acid receptors and exerts anticonvulsant action in mice. Pharmacol Biochem Behav 90: 712–716.R. MucenieceK. SalenieceJ. RumaksL. KrigereZ. Dzirkale2008Betulin binds to gamma-aminobutyric acid receptors and exerts anticonvulsant action in mice.Pharmacol Biochem Behav90712716
- 39. de Souza MT, Buzzi FC, Cechinel FV, Hess S, Della MF, et al. (2007) Phytochemical and antinociceptive properties of Matayba elaeagnoides Radlk. barks. Z Naturforsch [C] 62: 550–554.MT de SouzaFC BuzziFV CechinelS. HessMF Della2007Phytochemical and antinociceptive properties of Matayba elaeagnoides Radlk. barks.Z Naturforsch [C]62550554