MicroRNAs (miRNAs) are short, non-coding RNA regulators of protein coding genes. miRNAs play a very important role in diverse biological processes and various diseases. Many algorithms are able to predict miRNA genes and their targets, but their transcription regulation is still under investigation. It is generally believed that intragenic miRNAs (located in introns or exons of protein coding genes) are co-transcribed with their host genes and most intergenic miRNAs transcribed from their own RNA polymerase II (Pol II) promoter. However, the length of the primary transcripts and promoter organization is currently unknown.
We performed Pol II chromatin immunoprecipitation (ChIP)-chip using a custom array surrounding regions of known miRNA genes. To identify the true core transcription start sites of the miRNA genes we developed a new tool (CPPP). We showed that miRNA genes can be transcribed from promoters located several kilobases away and that their promoters share the same general features as those of protein coding genes. Finally, we found evidence that as many as 26% of the intragenic miRNAs may be transcribed from their own unique promoters.
Citation: Corcoran DL, Pandit KV, Gordon B, Bhattacharjee A, Kaminski N, Benos PV (2009) Features of Mammalian microRNA Promoters Emerge from Polymerase II Chromatin Immunoprecipitation Data. PLoS ONE4(4): e5279. https://doi.org/10.1371/journal.pone.0005279
Editor: Christopher K. Patil, Lawrence Berkeley National Laboratory, United States of America
Received: January 5, 2009; Accepted: March 23, 2009; Published: April 23, 2009
Copyright: © 2009 Corcoran et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by NIH-NLM award 1R01LM009657 and by NIH-NIAID contract N01AI50018. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
MicroRNAs (miRNAs) are short, ∼22 nt, single-stranded RNAs that act as regulators of genes' expression. By virtue of base complementarity, they bind to their target gene mRNAs and can block translation or accelerate their degradation . miRNAs have been implicated in a variety of human diseases ,  and more recent studies showed their association with particular cellular pathways .
Although miRNA genes play an important role in many biological processes, little is known about their transcriptional regulation. Currently, it is believed that most miRNA genes are transcribed by RNA polymerase II (Pol II) , , although some exceptions exist . A first step toward understanding miRNA regulation is to identify their transcription start sites (TSSs). Currently, only a small number of human miRNA genes have confirmed TSSs , , , which is insufficient for studying the promoter sequence features and for comparison with protein coding genes. Due to this lack of information, all studies attempting to analyze the miRNA core promoters have focused on the area immediately upstream of the computational prediction of the pri-miRNA , , . While these regions exhibit similar conservation patterns to the promoters of protein coding genes  their potential to act as promoters is still unknown. Identifying the active core miRNA promoters will thus allow us to study particular pri-miRNA characteristics such as transcript length and core promoter features. Recently, two studies that utilized high-throughput genomic techniques offered a first glimpse into the likely location and sequence characteristics of human miRNA TSSs , . In addition, two other studies involving high-throughput data from mouse and C. elegans offered insights on miRNA gene transcription in these species , .
Existing algorithms for modeling Pol II core promoters vary both in methodology and in performance. Previous algorithms have used transcription factor binding site frequencies , the size and location of CpG islands , and the physical properties of the DNA. Algorithmically, neural networks , relevance vector machines , and additive logistic regression with boosting have been applied .
To better understand the transcriptional regulation of miRNAs we performed chromatin immunoprecipitation (ChIP)-chip for the Pol II complex using a custom designed miRNA location array. After comparing different DNA features, we developed an efficient Support Vector Machine (SVM) based method for Pol II core promoter classification (Core Promoter Prediction Program, CPPP). We applied these tools to identify miRNA TSSs, better understand how intergenic and intragenic (i.e., intronic or exonic) miRNA genes are transcribed and to compare the features of their promoters with those of the protein coding genes.
Results and Discussion
Identification of regions containing pri-miRNA TSSs from Pol II ChIP-chip data
To identify the TSS for pri-miRNAs, ChIP-chip was performed on A549 lung epithelial cells with a Pol II-specific antibody, as described in Materials and Methods. Statistical analysis  was used to identify windows of 1 Kb in length that exhibit significant Pol II signals (immunoprecipitated DNA vs. background). The nearest statistically significant window to the 5′ end of each of the 531 known pre-miRNAs was further analyzed with our algorithm to predict whether it contained the miRNA TSS. The custom-made tiling array we used included 50 Kb upstream of each known miRNA gene (see Materials and Methods). This distance threshold is consistent with previous studies that showed high correlation of expression between miRNA genes located up to 50 Kb apart . Our method resulted in 34 intergenic pre-miRNAs or polycistronic pri-miRNAs having a statistically significant Pol II signal associated with them (Table 1). Regions with a significant Pol II signal that also overlapped the 5′ end of a known gene (as identified by the UCSC table browser , ) were excluded from subsequent analysis. This was necessary because the ChIP-chip data cannot distinguish shared core promoter regions. An example of the distribution of the Pol II binding signals and the identified TSS of the pri-miR-10a is presented in Figure 1.
The blue arrow represents the location and transcriptional direction of hsa-miR-10a. The red dashes represent the location and value of the ChIP-chip probes. TSS – transcription start site of this miRNA.
The miR-23a∼miR-27a∼miR24-2 cluster is probably the best-studied human intergenic pri-miRNA transcript. Lee et al.  have shown that the TSS for this cluster is located 124 nucleotides upstream of miR-23a, which our ChIP-chip data analysis confirmed. The ChIP-chip data was also able to confirm the previously reported pri-miRNA TSS listed by Fujita and Iba  for miR-21 (Table 1). The distance between the Pol II peaks and the location of the pre-miRNA varies substantially between genes, from a minimum of zero to a maximum of 40 Kb. The average and median values are 10.8 Kb and 8.7 Kb, respectively.
The analysis of the Pol II ChIP-chip data also provided insights into how intragenic (intronic, exonic) miRNAs are transcribed. Currently, it is believed that they are transcribed along with their host gene . Indeed, for many intragenic miRNAs the nearest significant upstream Pol II ChIP-chip peak region overlapped the 5′ region of their host gene (Table 2). These cases include a few miRNAs that were previously shown to be co-transcribed with their host gene, such as miR-146a  and the miR-17∼miR-18a∼miR-19a∼miR-20a∼miR-19b-1∼miR-92a-1 cluster . Interestingly, the analysis found that some of the intragenic miRNA genes may be transcribed by their own promoter (Table 3), which was also observed in the recent analysis by Ozsolak et al. . We note that in contrast with the promoters of intergenic miRNA genes, CpG islands were identified only in 3 of the 11 intragenic promoters. The distance between the Pol II peak and the beginning of the (intragenic) pre-miRNA gene also varies between zero and 41 Kb, but with a higher number of TSSs observed at longer distances (average and median = 19 Kb).
Modeling Pol II core promoter features with n-mers and weight matrices
In the following section, we describe the development of CPPP, a novel SVM-based method for prediction of Pol II TSSs. CPPP was used for the identification of the miRNA TSSs from the ChIP-chip data and for comparing the features of the miRNA promoters to those of protein coding gene promoters.
It is known that the genomic regions immediately upstream of the TSS of protein coding genes exhibit high levels of sequence conservation , , , , , which is probably related to the high concentration of cis-regulatory sites in this region . All of the existing algorithms for modeling Pol II core promoters have used this property to different extents. Generally one can model DNA target sites using either n-mer frequencies or weight matrices, commonly known as position-specific scoring matrices (PSSM) . The first class of methods (also termed enumerating or dictionary-based methods; e.g., , , , ) is better suited for representation of the binding preferences of those transcription factors that have a restricted set of DNA targets. n-mer frequencies have been used in the past to model Pol II core promoters either alone  or in conjunction with some promoter entropy measure . However, the DNA targets of most transcription factors are not highly conserved, which is the reason why PSSM models are widely used for representing DNA motifs. Regardless, using PSSMs for Pol II core promoter recognition has also its limitations. First, the currently known DNA motifs are redundant, not only because the available databases contain multiple matrices for the same factor, but also because structurally similar transcription factors are known to recognize similar “core” motifs , . Second, the binding preferences are known only for a small percent of the transcription factor proteins and protein complexes. For example, TRANSFAC database  currently has annotated 2,113 mammalian transcription factors, but it only contains 601 binding models. Third, even if the binding preference of a given transcription factor is known, the task of determining whether it binds to a given promoter is not trivial, mainly due to the high false positive prediction rate , . Despite the above limitations, PSSM models have been used extensively in the past for Pol II core promoter identification , , .
The problems of PSSM model redundancy and the relatively small number of transcription factors with known binding preferences can be diminished if one uses familial binding profiles (FBPs) . FBPs represent an “average” of the binding preferences of related transcription factors. They are based on the fact that transcription factors of the same structural group typically bind to similar sets of sequences. This method not only reduces the PSSM model redundancy, but also offers models for the transcription factors for which the binding preference is currently unknown (since the transcription factors with unknown preferences are likely to belong in one of the existing families). Sandelin and Wasserman initially built a set of 11 FBPs using a semi-manual method . In that study, the zinc finger proteins were excluded from the FBP construction due to their high degree of target promiscuity, which in turn makes them difficult to cluster correctly. More recently, Mahony et al.  used an automatic method to construct 17 FBPs. This set of FBPs includes all but the C2H2 the zinc finger (sub)family.
Using the same clustering method developed in Mahony et al.  we built 31 new FBPs from the C2H2 zinc finger proteins. These 31 new FBPs together with the 17 FBPs from the Mahony et al. study were used in the subsequent analysis.
Evaluating Core Promoter Features Using Support Vector Machines
In order to better understand how various features contribute to the characterization of the Pol II core promoters we compared them using an SVM , . The SVM methodology was chosen because it can combine multiple types of evidence (features) under the same general framework. In this study, we used (a) the n-mer frequencies (n = 3,4) and (b) matches to the set of 48 generalized DNA binding profiles (FBPs) as features of the SVM, and (c) the GC content. The reason for using GC content as an additional feature in the SVM training is that it seems to be a prominent feature in a subset of eukaryotic promoters .
Overall, we constructed and compared five SVM models: (1) FBPs only (48 features), (2) n-mers only (n = 3,4) (320 features), (3) FBPs+GC content, (4) n-mers+GC content, and (5) FBPs+n-mers+GC content. All models were trained on the same set of 3,015 verified core promoters of protein coding genes (positive examples; see Materials & Methods) and 3,015 randomly chosen intergenic sequences (negative examples; see Materials and Methods). Performance was measured by a 20× cross-validation in which 75% of the examples in each dataset were used for training and the remaining 25% for testing. The results are presented in Figure 2, and indicate that the n-mer-based models perform generally better than the FBP-based models, both in terms of sensitivity (percent of correctly predicted positive examples) and specificity (percent of true positive examples among all predictions). For example, the “n-mer only” SVM model (n = 3, 4) exhibited SN = 74.3% and SP = 86.1% compared to SN = 70.8% and SP = 82.2% of the “FBP only” model. It should be noted, however, that none of these differences is statistically significant. Based on these results, one may choose to use FBPs for this type of modeling, especially in species where the number of training examples is limited.
Sn – sensitivity, Sp – specificity.
The SVM results reported above were based on the dot plot kernel function (linear discriminator). Tests with polynomial (3rd order) and radial kernels gave the same or slightly worse results (data not shown). Also, all SVM models were constructed using random intergenic regions as background (see Materials and Methods) instead of the intronic regions previous studies have used . Evaluation with intronic sequences as background was found to be slightly worse (data not shown).
We note that other studies have occasionally reported better performance (e.g., , ). We believe this is due to the smaller size of the datasets they used and the type of promoters these datasets contained. For example, Gangal and Sharma  reported SN>87% and SP>86%, but the 800 promoter sequences in their dataset were all taken from EPD . EPD is highly enriched in promoters containing CpG islands (about 83% of the total.) A very powerful separation hyperplane can be created using these GC-rich promoters as positive set and the, generally AT-rich, intronic sequences as negative set. However this model is expected to perform poorly on non-CpG island promoters, as we will show. In our case, only half of the promoters in the training/testing dataset contained CpG islands. When the EPD dataset is used for training/testing in this study, our results are similar (intronic background) or slightly better (intergenic background) to those reported in Gangal and Sharma . Nevertheless, we found that partitioning the promoters to those containing CpG islands and those lacking CpG islands improves the results substantially (see below).
The effect of the presence or absence of CpG islands in the prediction efficiency of Pol II core promoters
In general, the frequency of CG dinucleotides in vertebrate genomes is lower than expected by chance . This is due to the frequent conversion of methylated-CG into TG . However, often the promoters of vertebrate genes contain stretches with high frequency of CG dinucleotides (CpG islands) . These regions are often defined as 200 nt or more with GC content greater than 50% . Ioshikhes and Zhang  have previously used this feature to predict the CpG island containing promoters with high efficiency. For this reason, we tested the prediction efficiency of the “n-mer only” and “FBP only” SVM models in mammalian core promoters in the presence or absence of CpG islands. Focus was placed on these two models because they are simpler than the composite model and their performance in the general dataset is the same or slightly better than the other models (Figure 2).
The positive training set was partitioned into CpG containing promoters (CpG+) and non-CpG promoters (CpG−), for each of which a n-mer-based and a FBP-based SVM model were calculated. The negative dataset contained equal number of randomly selected sequences from the intergenic parts of the genome (see Materials and Methods). The results demonstrate that if SVMs are trained in this way, then the prediction efficiency differs significantly between the two types of promoters. In particular, the “n-mer model” trained on CpG+ promoters exhibits SN = 94.8% (SD = 1.1%) and SP = 97.6% (SD = 1%) in the cross-validation tests. By contrast, when trained on CpG− promoters the “n-mer model” performs significantly worse (SN = 73.4%, SD = 2.6% and SP = 73.2%, SD = 2.9%) (Figure 3). The results with the “FBP model” are similar for both the CpG+ and CpG− trained models (Figure 3). Also, the results show that in general n-mer models perform slightly better than the corresponding FBP models regardless of the training (CpG+ or CpG− datasets) (Figure 3). Furthermore, the results show that n-mer-based models trained on CpG+ promoters tend to predict extremely well the CpG promoters (SN = 94.8%, SP = 97.6%), which agrees with previous reports . We have discovered that this better performance can be attributed to the GC content of these promoters (compared to the background), and this could be misleading. When intergenic sequences with similar GC content were used as negative dataset during training, the efficiency of the n-mer-based SVM on CpG+ promoters was reduced to values similar to the prediction of the CpG− promoters with the CpG− model (SN = 75.3% with SD = 2.4% and SP = 80.0% with SD = 2.0%). Since our main aim in this report is to discover important promoter features, not simply the features of the CpG islands, in the following analysis we use the seemingly less efficient models (i.e., n-mer SVMs trained on CpG+ vs. GC-normalized intergenic background and CpG− vs. random intergenic background).
Two SVM models were evaluated in the prediction of the CpG+ promoters: one with random intergenic background (CpG+/Rnd_bg) and one with intergenic background with similar GC content (CpG+/GC_bg). Sn – sensitivity, Sp – specificity.
The program ‘gist-fselect’ from the Gist package  was used to evaluate the significance of each of the features of core promoter regions (t-test metric p-value was used to determine significance) in CpG and non-CpG promoters. The top 20 features (ranked by the Fisher score of the package) are presented in Table 4. Interestingly, despite correcting for GC content, the most significant features for the CpG+ promoters were CG containing n-mers. Also of interest is the n-mer ‘CTG,’ which is present in the top 20 most significant features of both models.
Comparison of core promoters for protein coding and miRNA genes with SVM models
The ChIP-chip data showed that 34 of the intergenic miRNA genes had significant Pol II signals less than 50 Kb away. The 3 Kb regions surrounding the windows with the most significant Pol II peak were collected and the presence or absence of CpG islands was determined using the same method as in Zhao et al. . CpG islands were identified in about 55% of these promoters. Subsequently, the corresponding SVM model (trained on CpG+ or CpG− promoters of protein coding genes) was used across the significant ChIP-chip region to identify the top scoring 500 bp window that contains the predicted TSS. The CPPP algorithm identified a TSS in the upstream regions of 29 out of the 34 intergenic miRNA genes (Table 1). Each of the five intergenic miRNAs for which CPPP was unable to identify a core promoter contained a 500 bp region that scored just below the threshold cutoff for identifying a core promoter from a background sequence (data not shown).
The number of Pol II associated intergenic miRNA genes is not large enough to retrain the SVM models and calculate significant sequence features. However, we can test whether the most significant features in the promoters of the protein coding genes (Table 4) are also overrepresented in the miRNA promoters. Comparison of all n-mer frequencies of the CpG promoters of protein coding genes with those of the miRNA genes resulted in a statistically significant difference of 5 n-mers (‘CAC’, ‘GCAC’, ‘CGGT, ‘GTAC’, and ‘CTTA’; Wilcoxon signed-rank test; p-value<0.05 after Bonferroni correction). However, the only 4-mer in the list of the top 50 most important features for the model was ‘CAC’. For the non-CpG miRNA promoters, we found no features with a statistically different frequency when compared to that of the protein coding genes.
Computational analysis of potential promoters of intragenic miRNA genes
Intragenic miRNA genes are generally believed to be co-transcribed with their host genes. Overall, we found significant Pol II peaks associated with 43 intragenic miRNA genes or gene clusters. In 27 cases, the Pol II peak overlapped the promoter of the host gene (Table 2), but in 15 cases the Pol II peak was located within the host gene (Table 3). We scanned the ChIP identified regions with internal Pol II peaks with the corresponding SVM model (CpG+ or CpG−) and we found that 11 of these 15 intragenic genes contained a highly likely TSS region (Table 3.) This result indicates that 26% or more of the intragenic genes may be transcribed from their own promoter. In agreement with this finding, the miR-32 gene was previously shown to have a negative correlation with its host gene, C9orf5 . This is an important and interesting finding about the transcriptional regulation of intragenic miRNAs, although further biochemical validation is required.
Comparison with ChIP-seq data
Marson and colleagues  recently performed ChIP-seq experiments with four general transcription factors in human and mouse cells. They then combined their data with those from previous studies on epigenetic markers. Using a variety of features such as evolutionary conservation and distance of the peak from the known miRNA genes, they assigned an ad hoc score to each putative TSS. A positive score indicated that the TSS is likely to belong to the miRNA and a negative score indicated that the TSS likely belonged to another gene. Although their study is very different than ours in the methodology followed (ChIP-chip vs. ChIP-seq) and the proteins targeted by the ChIP, comparing the two can be useful. Overall, we found the two datasets are comparable with respect to miRNA promoter characteristics. Furthermore, we ran the CPPP algorithm on their data and verified most of their predicted promoters. In particular, there are 44 TSSs associated with intergenic miRNAs with positive Marson score (after removing some inconsistent or mis-annotated TSSs, see Materials and Methods); and CPPP scored all but three of them as likely to contain a core promoter (see Supplementary Data). The two datasets (ours and Marson's) include one miRNA gene and one miRNA cluster with well studied TSSs: cluster miR-23a∼miR-27a∼miR-24-2 , and gene miR-21 . CPPP correctly identified the location of both known TSSs, while the Marson dataset only found the correct TSS for the miR-23a cluster (see Supplementary Data.)
The prediction of miRNA TSSs and the understanding of the processes that affect their transcription is an essential step towards deciphering their role in regulatory networks. In this study, high-throughput Pol II ChIP-chip data were collected and used to infer miRNA TSSs. Analysis of these data showed that intergenic and some intragenic miRNAs are transcribed by Pol II at a distance that can be as large as 40 Kb from the pre-miRNA genes, indicating that pri-miRNA transcripts might be much longer than originally thought , , . We compared the most commonly used promoter features (n-mer frequencies and PSSM models) and found the n-mer frequencies to be generally better than the generalized PSSM models, at the cost of additional parameters. Also, in agreement with other studies , we found that CpG+ promoters are much easier to predict than CpG− and that core promoter prediction is more efficient when both models are used independently. However, we also found that this generally superior performance of the SVM models on CpG promoters vs. non-CpG promoters is due to the GC bias of the former. When a random background with similar GC content as the CpG+ promoters was used, the efficiency of the SVM model became similar to that of the CpG− model. This is a previously overlooked feature of the SVM training for core promoter recognition. Using the best performing SVM on our ChIP-chip data, we found that miRNA Pol II promoters contain most of the same features as the protein coding gene promoters.
Our results highlight the complexity and independence of the miRNA gene expression regulation and thus encourage more detailed studies in various cells, organs and physiological conditions. Our study gives a biochemical verification to previous statistical analyses that indicated that pri-miRNA transcripts can be tens of thousands of bases long . Finally, the finding that 26% or more of the intragenic miRNA genes may be transcribed by their own promoter also encourages much more detailed studies into their transcriptional regulation.
Elucidating the transcriptional networks that determine expression of miRNAs is critically important considering their important regulatory roles. miRNA location arrays may be useful tools in elucidating these networks
Materials and Methods
Chromatin Immunoprecipitation (ChIP-chip)
Approximately 108 A549 cells (American Type Culture Collection, Manassas, VA) were grown in F12K medium (Invitrogen, Carlsbad, CA) with 2 mM L-glutamine and 10% fetal bovine serum. Cells were incubated at 37°C in a humidified chamber supplemented with 5% CO2. Once 80% confluent, cells were serum starved overnight. Proteins were cross-linked to the DNA using fresh formaldehyde solution (50 mM Hepes-KOH pH 7.5, 100 mM NaCl, 1 mM EDTA pH 8.0, 0.5 mM EGTA pH 8.0, 11% Formaldehyde) for 10 min at room temperature. The formaldehyde was quenched with 2.5 M glycine for 5 min at room temperature. Cells were washed twice in PBS and harvested using a silicone scraper. Cells were centrifuged at 1,350×g for 5 minutes at 4°C and the pellet washed twice with PBS. The pellet was resuspended in 5 ml of lysis buffer 1 (50 mM Hepes-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100) and rocked at 4°C for 10 min. The cells were centrifuged at 1,350×g for 5 minutes at 4°C and the pellet resuspended in 5 ml of lysis buffer 2 (10 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA), rocked at room temperature for 10 min. The nuclei were pelleted by centrifuging at 1,350×g for 5 minutes at 4°C. The pellet was resuspended in 5 ml of lysis buffer 3 (10 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 0.1% Na-deoxycholate, 0.5% N-lauroylsarcosine). The cells were sonicated for 7 cycles of 30 seconds ON and 60 seconds OFF at a power 7 using a sonic dismembrator Model 100 (Fisher Scientific, Waltham, MA). The cells were centrifuged at 20,000×g for 10 minutes at 4°C and 50 µl of the supernatant was set aside as the whole cell extract (WCE). The rest of the supernatant was incubated overnight at 4°C with 100 µl of Dynal Protein G magnetic beads that had been pre-incubated with either 10 µg RNA polymerase II antibody (Abcam, Cambridge, MA) or 10 µg E2F-4 antibody (Santa Cruz Biotechnology, Santa Cruz, CA). The beads were washed 7 times in RIPA buffer (50 mM Hepes-KOH pH 7.6, 500 mM LiCl, 1 mM EDTA pH 8.0, 1% NP-40, 0.7% Na-deoxycholate) and once in Tris-EDTA containing 50 mM NaCl. Elution was done in elution buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA pH 8.0, 1% SDS) for 15 min at 65°C. Reversal of crosslinks of the immunoprecipitate (IP) and the WCE was done at 65°C overnight. Cellular RNA was digested with 0.2 mg/ml RNaseA (Invitrogen) at 37°C for 2 h followed by protein digestion with 0.2 mg/ml proteinase K (Invitrogen) at 55°C for 30 min. The DNA was purified by phenol∶chloroform∶isoamyl alcohol extraction and ethanol precipitation. Purified DNA was blunted using T4 DNA polymerase (New England Biolabs, Ipswich, MA) and ligated to 2 µM linkers using T4 DNA ligase (New England Biolabs). The IP and the WCE was amplified in two stages of PCR and purified by phenol∶chloroform∶isoamyl alcohol extraction and ethanol precipitation. 2 µg each of IP and WCE was labeled with Cy5-dUTP and Cy3-dUTP (Perkin Elmer, Waltham, MA) respectively. Labeling was carried out by random-primed Klenow-based extension using the CGH Labeling kit (Invitrogen). The samples were cleaned up using Invitrogen's CGH columns included in the kit. 5 µg each of IP and WCE were combined with cot-1 DNA and the 10× blocking agent and 2× hybridization buffer supplied in the Agilent Oligo aCGH/ChIP-on-chip Hybridization Kit (Agilent, Santa Clara, CA). Hybridization was carried out in Agilent's SureHyb chambers at 65°C for 40 h in the DNA Microarray Hybridization Oven (Agilent). The slides were washed using Oligo aCGH/ChIP-on-chip wash buffer 1 and 2 (Agilent) and scanned in the DNA microarray scanner (Agilent). The scanned images were processed using Agilent's Feature Extraction software version 9.5.3
Marson et al.  recently published a study where they combined different ChIP-seq datasets for multiple DNA binding proteins (or modifications of them) to unravel the transcriptional machinery of the miRNA genes in mouse and human cells. We analyzed their human dataset with our CPPP and compared their results with ours. Their original dataset contained TSSs associated with 101 intergenic miRNA genes or gene clusters. We excluded 19 of them from this analysis, because we found them to either overlap with promoters of protein coding genes (10 TSSs) or were located downstream of the corresponding miRNA gene (4 TSSs) or there were genes found between the miRNA gene and the TSS prediction (5 genes; see Supplementary Data.) We also converted the coordinates to the current version of the human genome (hg18) using the liftOver utility of the UCSC Genome Browser . This caused the miRNA-TSS distances to change significantly (more than 50 bp) for 7 of the 101 genes.
miRNA Location Array Design
The miRNA location array was custom-made by Agilent with AMADID (Agilent Microarray Design Identifier) 014119. The array is available on the 44 K design. The probes tile 100 Kb regions (∼200 bp spacing) surrounding each miRNA and only in non-repeat masked regions. The probes are 45–60-mers, Tm balanced and map to the Hg17 database. There are 41585 probes from ∼164 miRNA intervals. Control probes such as GD (gene desert), intensity controls (LACC) and some negative controls were also included.
Analysis of ChIP-chip Data
Median normalization of the log2 values of the ratio of signal to mock (precipitated DNA without antibody) was performed across the three-ChIP-chip arrays followed by a mean centralization to 0. Regions of Pol II binding were identified by the ChIPOTle sliding window method ; a window size of 1 Kb was used with a step size of 50 bp. The window was reported as significant if the p-value was below 0.05 after adjustment by the conservative Bonferroni correction method for multiple testing. Overlapping significant windows were combined and the region with the lowest p-value was reported.
Gene Coordinate and Sequence Collection
Pol II core promoters were extracted from two databases: Eukaryotic Promoter Database  and DBTSS . Between the two databases there were 3,015 unique human TSSs (1,744 from Eukaryotic Promoter Database and 1,271 from DBTSS as originally identified by Zhou et al ). The core promoter regions were partitioned into 1,445 that contained CpG islands and 1,570 that did not according to the method and threshold used in Zhao et al . For the training and testing of the various SVM models the area [−450, +50] surrounding the TSS was used as the positive dataset. An equal number of 500 bp genomic sequences, randomly selected from the intergenic regions of all chromosomes were used as the negative dataset for the CpG− model. A second set of sequences was collected as described by Zhao et al.  This dataset had GC content similar to the CpG island promoter dataset and was used as negative dataset for the CpG− model. Special care was given so that the randomly selected regions were not located within 3 Kb from the 5′ end of any annotated gene.
Genomic coordinates for all mRNA TSSs, mRNA introns and miRNA were collected from the UCSC table browser , . Intragenic miRNAs were identified as those found within an intron, exon or UTR of a mRNA and transcribed in the same orientation. All other miRNAs were labeled as intergenic.
Conceived and designed the experiments: NK PVB. Performed the experiments: DLC KVP. Analyzed the data: DLC KVP NK PVB. Contributed reagents/materials/analysis tools: DLC KVP DBG AB. Wrote the paper: DLC KVP AB NK PVB.
- 1. Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116: 281–297.DP Bartel2004MicroRNAs: genomics, biogenesis, mechanism, and function.Cell116281297
- 2. Jin P, Zarnescu DC, Ceman S, Nakamoto M, Mowrey J, et al. (2004) Biochemical and genetic interaction between the fragile X mental retardation protein and the microRNA pathway. Nat Neurosci 7: 113–117.P. JinDC ZarnescuS. CemanM. NakamotoJ. Mowrey2004Biochemical and genetic interaction between the fragile X mental retardation protein and the microRNA pathway.Nat Neurosci7113117
- 3. Thum T, Galuppo P, Wolf C, Fiedler J, Kneitz S, et al. (2007) MicroRNAs in the human heart: a clue to fetal gene reprogramming in heart failure. Circulation 116: 258–267.T. ThumP. GaluppoC. WolfJ. FiedlerS. Kneitz2007MicroRNAs in the human heart: a clue to fetal gene reprogramming in heart failure.Circulation116258267
- 4. Shalgi R, Lieber D, Oren M, Pilpel Y (2007) Global and local architecture of the mammalian microRNA-transcription factor regulatory network. PLoS Comput Biol 3: e131.R. ShalgiD. LieberM. OrenY. Pilpel2007Global and local architecture of the mammalian microRNA-transcription factor regulatory network.PLoS Comput Biol3e131
- 5. Lee Y, Kim M, Han J, Yeom KH, Lee S, et al. (2004) MicroRNA genes are transcribed by RNA polymerase II. EMBO J 23: 4051–4060.Y. LeeM. KimJ. HanKH YeomS. Lee2004MicroRNA genes are transcribed by RNA polymerase II.EMBO J2340514060
- 6. Rodriguez A, Griffiths-Jones S, Ashurst JL, Bradley A (2004) Identification of mammalian microRNA host genes and transcription units. Genome Res 14: 1902–1910.A. RodriguezS. Griffiths-JonesJL AshurstA. Bradley2004Identification of mammalian microRNA host genes and transcription units.Genome Res1419021910
- 7. Borchert GM, Lanier W, Davidson BL (2006) RNA polymerase III transcribes human microRNAs. Nat Struct Mol Biol 13: 1097–1101.GM BorchertW. LanierBL Davidson2006RNA polymerase III transcribes human microRNAs.Nat Struct Mol Biol1310971101
- 8. Liu N, Williams AH, Kim Y, McAnally J, Bezprozvannaya S, et al. (2007) An intragenic MEF2-dependent enhancer directs muscle-specific expression of microRNAs 1 and 133. Proc Natl Acad Sci U S A 104: 20844–20849.N. LiuAH WilliamsY. KimJ. McAnallyS. Bezprozvannaya2007An intragenic MEF2-dependent enhancer directs muscle-specific expression of microRNAs 1 and 133.Proc Natl Acad Sci U S A1042084420849
- 9. Woods K, Thomson JM, Hammond SM (2007) Direct regulation of an oncogenic micro-RNA cluster by E2F transcription factors. J Biol Chem 282: 2130–2134.K. WoodsJM ThomsonSM Hammond2007Direct regulation of an oncogenic micro-RNA cluster by E2F transcription factors.J Biol Chem28221302134
- 10. Megraw M, Baev V, Rusinov V, Jensen ST, Kalantidis K, et al. (2006) MicroRNA promoter element discovery in Arabidopsis. RNA 12: 1612–1619.M. MegrawV. BaevV. RusinovST JensenK. Kalantidis2006MicroRNA promoter element discovery in Arabidopsis.RNA1216121619
- 11. Saini HK, Griffiths-Jones S, Enright AJ (2007) Genomic analysis of human microRNA transcripts. Proc Natl Acad Sci U S A 104: 17719–17724.HK SainiS. Griffiths-JonesAJ Enright2007Genomic analysis of human microRNA transcripts.Proc Natl Acad Sci U S A1041771917724
- 12. Zhou X, Ruan J, Wang G, Zhang W (2007) Characterization and identification of microRNA core promoters in four model species. PLoS Comput Biol 3: e37.X. ZhouJ. RuanG. WangW. Zhang2007Characterization and identification of microRNA core promoters in four model species.PLoS Comput Biol3e37
- 13. Mahony S, Corcoran DL, Feingold E, Benos PV (2007) Regulatory conservation of protein coding and microRNA genes in vertebrates: lessons from the opossum genome. Genome Biol 8: R84.S. MahonyDL CorcoranE. FeingoldPV Benos2007Regulatory conservation of protein coding and microRNA genes in vertebrates: lessons from the opossum genome.Genome Biol8R84
- 14. Marson A, Levine SS, Cole MF, Frampton GM, Brambrink T, et al. (2008) Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells. Cell 134: 521–533.A. MarsonSS LevineMF ColeGM FramptonT. Brambrink2008Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells.Cell134521533
- 15. Ozsolak F, Poling LL, Wang Z, Liu H, Liu XS, et al. (2008) Chromatin structure analyses identify miRNA promoters. Genes Dev 22: 3172–3183.F. OzsolakLL PolingZ. WangH. LiuXS Liu2008Chromatin structure analyses identify miRNA promoters.Genes Dev2231723183
- 16. Martinez NJ, Ow MC, Barrasa MI, Hammell M, Sequerra R, et al. (2008) A C. elegans genome-scale microRNA network contains composite feedback motifs with high flux capacity. Genes Dev 22: 2535–2549.NJ MartinezMC OwMI BarrasaM. HammellR. Sequerra2008A C. elegans genome-scale microRNA network contains composite feedback motifs with high flux capacity.Genes Dev2225352549
- 17. Prestridge DS (1995) Predicting Pol II promoter sequences using transcription factor binding sites. J Mol Biol 249: 923–932.DS Prestridge1995Predicting Pol II promoter sequences using transcription factor binding sites.J Mol Biol249923932
- 18. Ioshikhes IP, Zhang MQ (2000) Large-scale human promoter mapping using CpG islands. Nat Genet 26: 61–63.IP IoshikhesMQ Zhang2000Large-scale human promoter mapping using CpG islands.Nat Genet266163
- 19. Ohler U, Niemann H, Liao G, Rubin GM (2001) Joint modeling of DNA sequence and physical properties to improve eukaryotic promoter recognition. Bioinformatics 17: Suppl 1S199–206.U. OhlerH. NiemannG. LiaoGM Rubin2001Joint modeling of DNA sequence and physical properties to improve eukaryotic promoter recognition.Bioinformatics17Suppl 1S199206
- 20. Down TA, Hubbard TJ (2002) Computational detection and location of transcription start sites in mammalian genomic DNA. Genome Res 12: 458–461.TA DownTJ Hubbard2002Computational detection and location of transcription start sites in mammalian genomic DNA.Genome Res12458461
- 21. Zhao X, Xuan Z, Zhang MQ (2007) Boosting with stumps for predicting transcription start sites. Genome Biol 8: R17.X. ZhaoZ. XuanMQ Zhang2007Boosting with stumps for predicting transcription start sites.Genome Biol8R17
- 22. Buck MJ, Nobel AB, Lieb JD (2005) ChIPOTle: a user-friendly tool for the analysis of ChIP-chip data. Genome Biol 6: R97.MJ BuckAB NobelJD Lieb2005ChIPOTle: a user-friendly tool for the analysis of ChIP-chip data.Genome Biol6R97
- 23. Baskerville S, Bartel DP (2005) Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes. RNA 11: 241–247.S. BaskervilleDP Bartel2005Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes.RNA11241247
- 24. Karolchik D, Hinrichs AS, Furey TS, Roskin KM, Sugnet CW, et al. (2004) The UCSC Table Browser data retrieval tool. Nucleic Acids Res 32: D493–496.D. KarolchikAS HinrichsTS FureyKM RoskinCW Sugnet2004The UCSC Table Browser data retrieval tool.Nucleic Acids Res32D493496
- 25. Kent WJ, Sugnet CW, Furey TS, Roskin KM, Pringle TH, et al. (2002) The human genome browser at UCSC. Genome Res 12: 996–1006.WJ KentCW SugnetTS FureyKM RoskinTH Pringle2002The human genome browser at UCSC.Genome Res129961006
- 26. Fujita S, Iba H (2008) Putative promoter regions of miRNA genes involved in evolutionarily conserved regulatory systems among vertebrates. Bioinformatics 24: 303–308.S. FujitaH. Iba2008Putative promoter regions of miRNA genes involved in evolutionarily conserved regulatory systems among vertebrates.Bioinformatics24303308
- 27. Taganov KD, Boldin MP, Chang KJ, Baltimore D (2006) NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. Proc Natl Acad Sci U S A 103: 12481–12486.KD TaganovMP BoldinKJ ChangD. Baltimore2006NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses.Proc Natl Acad Sci U S A1031248112486
- 28. Cooper SJ, Trinklein ND, Anton ED, Nguyen L, Myers RM (2006) Comprehensive analysis of transcriptional promoter structure and function in 1% of the human genome. Genome Res 16: 1–10.SJ CooperND TrinkleinED AntonL. NguyenRM Myers2006Comprehensive analysis of transcriptional promoter structure and function in 1% of the human genome.Genome Res16110
- 29. Mahony S, Auron PE, Benos PV (2007) DNA familial binding profiles made easy: comparison of various motif alignment and clustering strategies. PLoS Comput Biol 3: e61.S. MahonyPE AuronPV Benos2007DNA familial binding profiles made easy: comparison of various motif alignment and clustering strategies.PLoS Comput Biol3e61
- 30. Taylor J, Tyekucheva S, King DC, Hardison RC, Miller W, et al. (2006) ESPERR: learning strong and weak signals in genomic sequence alignments to identify functional elements. Genome Res 16: 1596–1604.J. TaylorS. TyekuchevaDC KingRC HardisonW. Miller2006ESPERR: learning strong and weak signals in genomic sequence alignments to identify functional elements.Genome Res1615961604
- 31. Carninci P, Kasukawa T, Katayama S, Gough J, Frith MC, et al. (2005) The transcriptional landscape of the mammalian genome. Science 309: 1559–1563.P. CarninciT. KasukawaS. KatayamaJ. GoughMC Frith2005The transcriptional landscape of the mammalian genome.Science30915591563
- 32. Prestridge DS, Burks C (1993) The density of transcriptional elements in promoter and non-promoter sequences. Hum Mol Genet 2: 1449–1453.DS PrestridgeC. Burks1993The density of transcriptional elements in promoter and non-promoter sequences.Hum Mol Genet214491453
- 33. Stormo GD (2000) DNA binding sites: representation and discovery. Bioinformatics 16: 16–23.GD Stormo2000DNA binding sites: representation and discovery.Bioinformatics161623
- 34. Bussemaker HJ, Li H, Siggia ED (2000) Building a dictionary for genomes: identification of presumptive regulatory sites by statistical analysis. Proc Natl Acad Sci U S A 97: 10096–10100.HJ BussemakerH. LiED Siggia2000Building a dictionary for genomes: identification of presumptive regulatory sites by statistical analysis.Proc Natl Acad Sci U S A971009610100
- 35. Rigoutsos I, Floratos A (1998) Combinatorial pattern discovery in biological sequences: The TEIRESIAS algorithm. Bioinformatics 14: 55–67.I. RigoutsosA. Floratos1998Combinatorial pattern discovery in biological sequences: The TEIRESIAS algorithm.Bioinformatics145567
- 36. van Helden J, Andre B, Collado-Vides J (1998) Extracting regulatory sites from the upstream region of yeast genes by computational analysis of oligonucleotide frequencies. J Mol Biol 281: 827–842.J. van HeldenB. AndreJ. Collado-Vides1998Extracting regulatory sites from the upstream region of yeast genes by computational analysis of oligonucleotide frequencies.J Mol Biol281827842
- 37. Wang T, Stormo GD (2005) Identifying the conserved network of cis-regulatory sites of a eukaryotic genome. Proc Natl Acad Sci U S A 102: 17400–17405.T. WangGD Stormo2005Identifying the conserved network of cis-regulatory sites of a eukaryotic genome.Proc Natl Acad Sci U S A1021740017405
- 38. Gangal R, Sharma P (2005) Human pol II promoter prediction: time series descriptors and machine learning. Nucleic Acids Res 33: 1332–1336.R. GangalP. Sharma2005Human pol II promoter prediction: time series descriptors and machine learning.Nucleic Acids Res3313321336
- 39. Sandelin A, Wasserman WW, Lenhard B (2004) ConSite: web-based prediction of regulatory elements using cross-species comparison. Nucleic Acids Res 32: W249–252.A. SandelinWW WassermanB. Lenhard2004ConSite: web-based prediction of regulatory elements using cross-species comparison.Nucleic Acids Res32W249252
- 40. Wingender E (2004) TRANSFAC, TRANSPATH and CYTOMER as starting points for an ontology of regulatory networks. In Silico Biol 4: 55–61.E. Wingender2004TRANSFAC, TRANSPATH and CYTOMER as starting points for an ontology of regulatory networks.In Silico Biol45561
- 41. Corcoran DL, Feingold E, Dominick J, Wright M, Harnaha J, et al. (2005) Footer: a quantitative comparative genomics method for efficient recognition of cis-regulatory elements. Genome Res 15: 840–847.DL CorcoranE. FeingoldJ. DominickM. WrightJ. Harnaha2005Footer: a quantitative comparative genomics method for efficient recognition of cis-regulatory elements.Genome Res15840847
- 42. Loots GG, Ovcharenko I, Pachter L, Dubchak I, Rubin EM (2002) rVista for comparative sequence-based discovery of functional transcription factor binding sites. Genome Res 12: 832–839.GG LootsI. OvcharenkoL. PachterI. DubchakEM Rubin2002rVista for comparative sequence-based discovery of functional transcription factor binding sites.Genome Res12832839
- 43. Ohler U (2006) Identification of core promoter modules in Drosophila and their application in accurate transcription start site prediction. Nucleic Acids Res 34: 5943–5950.U. Ohler2006Identification of core promoter modules in Drosophila and their application in accurate transcription start site prediction.Nucleic Acids Res3459435950
- 44. Sandelin A, Wasserman WW (2004) Constrained binding site diversity within families of transcription factors enhances pattern discovery bioinformatics. J Mol Biol 338: 207–215.A. SandelinWW Wasserman2004Constrained binding site diversity within families of transcription factors enhances pattern discovery bioinformatics.J Mol Biol338207215
- 45. Brown MP, Grundy WN, Lin D, Cristianini N, Sugnet CW, et al. (2000) Knowledge-based analysis of microarray gene expression data by using support vector machines. Proc Natl Acad Sci U S A 97: 262–267.MP BrownWN GrundyD. LinN. CristianiniCW Sugnet2000Knowledge-based analysis of microarray gene expression data by using support vector machines.Proc Natl Acad Sci U S A97262267
- 46. Chapelle O, Haffner P, Vapnik VN (1999) Support vector machines for histogram-based image classification. IEEE Trans Neural Netw 10: 1055–1064.O. ChapelleP. HaffnerVN Vapnik1999Support vector machines for histogram-based image classification.IEEE Trans Neural Netw1010551064
- 47. Abeel T, Saeys Y, Bonnet E, Rouze P, Van de Peer Y (2008) Generic eukaryotic core promoter prediction using structural features of DNA. Genome Res 18: 310–323.T. AbeelY. SaeysE. BonnetP. RouzeY. Van de Peer2008Generic eukaryotic core promoter prediction using structural features of DNA.Genome Res18310323
- 48. Schmid CD, Perier R, Praz V, Bucher P (2006) EPD in its twentieth year: towards complete promoter coverage of selected model organisms. Nucleic Acids Res 34: D82–85.CD SchmidR. PerierV. PrazP. Bucher2006EPD in its twentieth year: towards complete promoter coverage of selected model organisms.Nucleic Acids Res34D8285
- 49. Bird AP (1980) DNA methylation and the frequency of CpG in animal DNA. Nucleic Acids Res 8: 1499–1504.AP Bird1980DNA methylation and the frequency of CpG in animal DNA.Nucleic Acids Res814991504
- 50. Bird A (1999) DNA methylation de novo. Science 286: 2287–2288.A. Bird1999DNA methylation de novo.Science28622872288
- 51. Gardiner-Garden M, Frommer M (1987) CpG islands in vertebrate genomes. J Mol Biol 196: 261–282.M. Gardiner-GardenM. Frommer1987CpG islands in vertebrate genomes.J Mol Biol196261282
- 52. Kuhn RM, Karolchik D, Zweig AS, Trumbower H, Thomas DJ, et al. (2007) The UCSC genome browser database: update 2007. Nucleic Acids Res 35: D668–673.RM KuhnD. KarolchikAS ZweigH. TrumbowerDJ Thomas2007The UCSC genome browser database: update 2007.Nucleic Acids Res35D668673
- 53. Wakaguri H, Yamashita R, Suzuki Y, Sugano S, Nakai K (2008) DBTSS: database of transcription start sites, progress report 2008. Nucleic Acids Res 36: D97–101.H. WakaguriR. YamashitaY. SuzukiS. SuganoK. Nakai2008DBTSS: database of transcription start sites, progress report 2008.Nucleic Acids Res36D97101