Cotton varieties expressing Cry proteins derived from the soil bacterium Bacillus thuringiensis (Bt) are grown worldwide for the management of pest Lepidoptera. To prevent non-target pest outbreaks and to retain the biological control function provided by predators and parasitoids, the potential risk that Bt crops may pose to non-target arthropods is addressed prior to their commercialization. Aphids play an important role in agricultural systems since they serve as prey or host to a number of predators and parasitoids and their honeydew is an important energy source for several arthropods. To explore possible indirect effects of Bt crops we here examined the impact of Bt cotton on aphids and their honeydew.
In climate chambers we assessed the performance of cotton aphids, Aphis gossypii Glover (Hemiptera: Aphididae) when grown on three Indian Bt (Cry1Ac) cotton varieties (MECH 12, MECH 162, MECH 184) and their non-transformed near isolines. Furthermore, we examined whether aphids pick up the Bt protein and analyzed the sugar composition of aphid honeydew to evaluate its suitability for honeydew-feeders.
Plant transformation did not have any influence on aphid performance. However, some variation was observed among the three cotton varieties which might partly be explained by the variation in trichome density. None of the aphid samples contained Bt protein. As a consequence, natural enemies that feed on aphids are not exposed to the Cry protein. A significant difference in the sugar composition of aphid honeydew was detected among cotton varieties as well as between transformed and non-transformed plants. However, it is questionable if this variation is of ecological relevance, especially as honeydew is not the only sugar source parasitoids feed on in cotton fields.
Citation: Lawo NC, Wäckers FL, Romeis J (2009) Indian Bt Cotton Varieties Do Not Affect the Performance of Cotton Aphids. PLoS ONE 4(3): e4804. https://doi.org/10.1371/journal.pone.0004804
Editor: Hany A. El-Shemy, Cairo University, Egypt
Received: October 15, 2008; Accepted: January 28, 2009; Published: March 12, 2009
Copyright: © 2009 Lawo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The study was compiled within the Pulse Network of the Indo-Swiss Collaboration in Biotechnology (ISCB). The study was funded by the Swiss Agency for Development and Cooperation (SDC) in Berne, Switzerland, and the Department of Biotechnology (DBT) in Delhi, India. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Heliothine caterpillars, such as Helicoverpa spp. (Lepidoptera: Noctuidae) or Heliothis virescens (Fabricius) (Lepidoptera: Noctuidae), and the pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), are key pests of cotton worldwide. To control these polyphagous herbivores, farmers routinely use large amounts of broad-spectrum chemical insecticides, killing many non-target arthropods in the process. However, since heliothine caterpillars have a history of developing resistance to almost all the insecticides used for their control –, alternative control methods have to be developed. One option is the use of insect-resistant genetically engineered (GE) varieties expressing lepidopteran-active Cry proteins derived from the soil bacterium Bacillus thuringiensis (Bt). So-called Bt cotton plants are grown commercially since 1996. Most of today's varieties express the Bt protein Cry1Ac either alone or in combination with Cry2Ab, protecting plants from damage by the main pest Lepidoptera .
In 2007, Bt cotton was grown in nine countries (USA, Argentina, Brazil, India, China, South Africa, Australia, Mexico, and Colombia) . As cotton is among the most intensively sprayed of all field crops, the introduction of Bt cotton has had a tremendous impact in terms of reducing insecticide use resulting in economic, environmental and human health benefits , . The first eleven years of Bt cotton production (1996–2006) have resulted in a 22.9% reduction in insecticide active ingredient application in cotton world wide . Insecticide reductions were most significant in India and China where the improved pest control also related to significant increases in yield , , . In India, Bt cotton hybrids expressing the cry1Ac gene are cultivated on an increasing area since their introduction in 2002. Almost tripling the area to 3.8 million hectares in 2006, India became the largest Bt cotton growing country in the world and in 2007, 131 Bt cotton hybrids were grown on a total of 6.2 million hectares .
Due to the reduction of broad spectrum insecticides in Bt cotton, herbivores which are not targeted by the Bt protein survive and occasionally reach pest status . To retain the biological control function provided by naturally occurring antagonists of herbivores –i.e. predators and parasitoids– and to prevent non-target pest outbreaks, the potential risk that GE crops may pose to natural enemies is addressed as part of the environmental risk assessment prior to the commercial release of any novel GE crop , . Several studies examined the effect of Bt crops on herbivores and arthropod natural enemies in recent years confirming the highly selective mode of action of the deployed Bt Cry proteins , .
Aphids generally play an important role in agricultural food webs since they serve as hosts or prey for a variety of parasitoids and predators. Consequently, the question whether aphids are affected by the Bt crop and whether they expose their natural enemies to the plant-expressed Bt protein is of high relevance. Studies available to date provide no evidence that Bt crops, expressing Cry1A proteins for the control of pest Lepidoptera, cause direct adverse effects on aphids , . This is not surprising, since the Bt protein does not appear to be ingested by aphids which feed on phloem-sap –. Surprisingly, two studies have reported considerable amounts of Bt Cry proteins in aphid samples , .
Further, aphids and other phloem feeders produce honeydew which is an important source of carbohydrates for sugar feeding arthropods, including hymenopteran parasitoids and aphid predators , . Sugars can enhance parasitoid reproductive fitness by increasing their longevity, fecundity and/or parasitism rate –. However, honeydew can be a relatively unsuitable sugar source as a result of unfavorable sugar composition , , . Honeydew nutrient composition could also be altered as a result of plant transformation.
Therefore, we investigated in standardized laboratory bioassays if the performance of the cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), was affected on three Indian Bt cotton varieties (MECH 12, MECH 162, MECH 184), expressing the Cry1Ac protein and their corresponding non-transformed near isolines. We clarified whether aphids pick up the Bt protein and we examined several aphid life-table parameters,. Furthermore, to gain insight into the impact of Bt on the nutrient composition of aphid honeydew the sugar composition of honeydew was examined.
With one exception, statistical analyses showed neither a Bt-transformation nor a cotton variety effect for any of the aphid life-table parameters assessed (three-way ANOVA; P>0.05; Table 1). The exception was a significant variety effect for the number of nymphs produced during a time span equaling the nymphal developmental time (FD) (F2,147 = 3.50; P = 0.033) which appears to be due to a discrepancy between the varieties MECH 12 and MECH 184. A significant experimental effect was calculated for the parameters FD, daily fecundity (DF), and total longevity (TL); however, no interaction among the different factors occurred.
There was a significant difference in the trichome density among the six different cotton plants (Kruskal-Wallis ANOVA; χ2 = 23.07; df = 5; P = 0.033) (Fig. 1). Conducting pair-wise comparisons, a significantly greater trichome density was found for Bt MECH 184 compared to the other two Bt cotton varieties (Mann-Whitney U-test; Bt MECH 12; U = 0.00; P = 0.015 and Bt MECH 162; U = 0.00; P = 0.010). For the control varieties a higher trichome density was observed for MECH 184 compared to MECH 12 (U = 2.00; P = 0.010). No difference in the trichome density was observed between Bt and non-Bt leaves for any of the three pairs (P>0.05). Remarkably, Bt cotton plants showed a considerably reduced variability in trichome densities compared to Bt plants (Fig. 1).
Boxplot figures showing the median trichome density per cm2 (n = 6 to 8). Brackets indicate a significant difference between two treatments; *, P<0.05; **, P<0.01. The outlier range is the range of values that fall above the upper outlier limit (+1.5×the height of the box) and below the lower outlier limit (−1.5×the height of the box).
Quantification of Cry1Ac protein in leaves and aphids
Leaves of cotton plants on which aphids had fed during the bioassay in which aphid performance was evaluated, expressed the Cry1Ac protein at the following levels (mean±SE); Bt MECH 12: 0.58±0.060 µg Cry1Ac/g f.w., Bt MECH 162: 0.73±0.089 µg Cry1Ac/g f.w. and Bt MECH 184: 0.82±0.065 µg Cry1Ac/g f.w.
All ELISA readings revealed that aphids that had been kept on Bt cotton did not contain detectable Cry1Ac protein; i.e. readings were below the limit of detection (LOD) of 0.002 µg/g f.w. Leaves from which aphids were collected for ELISA analysis expressed the following amounts of Cry1Ac: Bt MECH 12: 0.34±0.100 µg Cry1Ac/g f.w., Bt MECH 162: 0.62±0.191 µg Cry1Ac/g f.w. and Bt MECH 184: 0.38±0.156 µg Cry1Ac/g f.w. As expected, none of the non-Bt cotton leaves or aphid samples contained any Bt protein.
Sugar analyses in honeydew
As it was not possible to determine the exact amount of sugar in honeydew samples in µg/ml, the sugar composition was presented as percentage of total sugar content.
A total of eleven sugars were found in the aphid honeydew. Dominant were the phloem sugars, sucrose and fructose, as well as the aphid-synthesized sugar erlose, that together made up 73% (Bt MECH 184) to 94% (MECH 162) of the total sugar content. Smaller amounts of glucose, trehalose, maltose, mannitol, melibiose, and stachyose were detected in all samples. Sugar composition appeared to differ between Bt and non-Bt plants as well as among varieties (Fig. 2). Whereas, fructose levels were higher in the honeydew from non-Bt cotton, glucose was amplified in honeydew from Bt cotton (especially for Bt MECH 184). Interestingly, erlose levels differed noticeably among the cotton varieties. While it was the dominant sugar in the honeydew collected from MECH 162 and Bt MECH 162, a much lower proportion of erlose was found in the honeydew of MECH 184 and Bt MECH 184. The greatest proportion of maltose was detected in MECH 12 and sorbitol was measured in all non-Bt samples (even though at very low levels) but in none of the Bt varieties. Raffinose occurred in just two out of the six treatments (MECH 12 and Bt MECH 162).
Honeydew was collected over a 5 to 6 h interval from either Bt (Bt MECH 12, Bt MECH 162, Bt MECH 184) or the corresponding non-Bt cotton plants (MECH 12, MECH 162, MECH 184) (n = 5 to 7). Lactose, melezitose, mannose, rhamnose, and galactose were not found in any of the samples.
Performing a Principle Components Analysis (PCA) to visualize the data, a negative correlation between the sugars with the greatest influence on data variability (those with the longest vector, namely erlose, glucose, and fructose) could be observed. Glucose, sucrose, and trehalose were positively correlated with each other and negatively with fructose, maltose, and raffinose (Fig. 3 A). A positive correlation among the amount of erlose and sorbitol and a negative correlation with melebiose, stachyose, and mannitol were observed in the data set. Looking at clusters in the biplot graphic, it was assumed that the first axis was best explained by the factor cotton variety (56%) and the second axis by the factor Bt-transformation (19%; Fig. 3 A). Performing a Redundancy Analysis (RDA) to analyze the influence of the evaluated explanatory variables [transformation (Bt/non-Bt) and variety (MECH 12, MECH 162, MECH 184)] showed a strong positive correlation between erlose, trehalose, raffinose, and stachyose with the variety MECH 162, and a negative correlation with the variety MECH 184 (Fig. 3 B). Glucose, trehalose, raffinose, and stachyose were positively correlated with Bt cotton plants, and negatively with the variety MECH 12. The sugars mannitol, melebiose, and sucrose were positively correlated with the variety MECH 184 and negatively with the variety MECH 162. Sorbitol, maltose and fructose were positively correlated with the variety MECH 12 and non-Bt cotton plants and negatively with Bt plants. The Monte Carlo permutation test revealed a significant difference in sugar composition of honeydew due to the variety MECH 184 (F = 8.60; P = 0.001) as well as the Bt transformation (F = 3.97; P = 0.004; Fig. 3 B). The fact that the first two axes of the RDA only explained 32% of the variance (first axis = 0.249; second axis = 0.066), indicated that there were variables other than transformation and variety that could explain the differences in honeydew sugar composition.
Aphids were fed on Bt (Bt MECH 12, Bt MECH 162, Bt MECH 184) or the corresponding non-Bt cotton plants (MECH 12, MECH 162, MECH 184). Distribution of sugar composition shown in (A) the ordination biplot of a Principal Component Analysis (PCA) (eigenvalues: axis 1: 0.560, axis 2: 0.193) and (B) a Redundancy Analysis (RDA) (eigenvalues: axis 1: 0.249, axis 2: 0.066). The straight lines of the vectors represent the influence of the different sugars (species; Fig. 3 A; B), the triangles the centroids of the environmental variables (variety and Bt; Fig. 3 B). Sugars were expressed as percentage of total sugar.
Aphid performance did not differ between Bt and non-Bt cotton plants and none of the aphid life-table parameters assessed was influenced by the expression of the Bt protein. However, there was a significant difference among cotton varieties for the number of nymphs produced during a time span equaling the nymphal developmental time (FD). Furthermore, a slight variation in the rm-values suggested a small difference among cotton varieties. As the rm is difficult to interpret, the formula was used to calculate aphid population growth during one week. According to this formula, a population would have increased by a factor of ten on Bt and non-Bt cotton plants of the variety MECH 12 while population increase would have been more pronounced on the varieties MECH 162 (factor 12 or 13) and MECH 184 (factor 13). To detect differences among cotton varieties is not surprising since disparities are known to be caused by different plant characteristics, e.g. gossypol level  and trichome density . The latter effect was also found in our study. While a previous study reported differences in the trichome density between Bt and non-Bt cotton plants , this could not be confirmed for the three cotton varieties used in our study. It was noticeable, though, that the trichome density of Bt plants showed considerably less variation as compared to non-Bt plants. The reasons for this discrepancy are not known.
While a previous greenhouse study addressing the performance of A. gossypii on Bt and non-Bt cotton plants showed a variation in some life-table parameters among three consecutive generations , studies comparing aphid populations in Bt and non-Bt cotton fields gave inconsistent results. While some studies recorded no difference in aphid populations –, others found either increased ,  or decreased aphid densities  in the Bt crop. Given the results from the greenhouse/climate chamber studies, changes in aphid populations in cotton fields are unlikely directly attributable to the expression of the Bt Cry protein. Rather they may be due to other confounding factors, such as changes in the use of insecticides or increased overall health of the Bt crop , . In Bt maize, a study has shown aphids to perform significantly better (expressed as increase in aphid numbers) on different Bt maize varieties with different transformation events than on their respective non-transformed control varieties . While slight Bt maize effects on aphid performance had earlier been reported , other greenhouse studies did not show evidence of such effects , , . In the field, no or only minimal differences in aphid densities on Bt and non-Bt maize have been recorded , .
Our ELISA analyses revealed that none of the aphid samples contained detectable Bt protein. This finding is in accordance with many other studies which reported either no Bt protein or only trace amounts of protein in sap-sucking insects of the order Hemiptera after feeding on different Bt plants, including maize –, , oilseed rape , cotton , and rice , . In contrast to the studies listed above, Burgio et al.  detected 3% of the Cry1Ac protein content expressed by Bt oilseed rape (plant expression level: 64 ng Cry1Ac/g f.w.) in all of four aphid samples that had been collected in the greenhouse, but only in one out of eight samples collected from plants kept in a climate chamber (2% of the Cry1Ac protein content expressed by Bt oilseed rape). Even higher levels were reported from greenhouse studies by Zhang et al.  conducting ELISA analyses of aphids which previously had fed on a medium (plant expression level: 49 ng Cry1A/g f.w.) or a high (plant expression level: 94 ng Cry1A/g f.w.) expressing Bt cotton line. Surprising in this study was the fact that all ten aphid samples contained Bt protein after feeding on the medium Bt-expressing line, compared to only four out of ten samples after feeding on the high Bt-expressing line. Furthermore the positive aphid samples from the medium expressing Bt cotton plants contained 12% of the Bt content present in the plant while the aphid samples collected from the high-expressing Bt cotton contained only 4% of the Bt amount found in the plants. It is notable that (with one exception) all positive samples in the two studies listed above were collected in the greenhouse. We argue that one likely reason for the Bt proteins detected in these studies is contamination of the samples by other herbivores such as spider mites or thrips or their feces which contain large amounts of Bt protein , , . In a preliminary study, we had collected aphids from Bt cotton that was contaminated with thrips. Great care was taken to check all aphid samples under the binocular microscope, both before releasing the aphids on the cotton plants and again on recollection from the plants to ensure that all herbivores other than aphids were removed. Nevertheless subsequent ELISA analyses still detected some Bt protein in eleven out of twelve samples (0.02 to 0.06 µg Cry1Ac/g f.w. aphids, corresponding to 13–25% of the amount detected in cotton leaves; Lawo unpublished data). These protein levels were 10 to 30 times higher than the limit of detection of the ELISA used here. These findings underline that very low levels of contamination are sufficient to produce false positives, especially in samples that contain only traces, or no Bt protein, like aphids.
To prevent potential thrips or spider mite infestations of the plants and thus contamination of the aphid samples, our experiment was conducted in a climate chamber instead of a greenhouse. No Bt protein could be detected in any of the aphid samples collected in the climate chamber, even though the Bt cotton plants were expressing similar levels of Bt protein compared to greenhouse-grown plants. It can thus be concluded that aphids feeding on Bt cotton do not ingest measurable amounts of Bt protein and consequently cannot pass it on to their natural enemies. The Cry1Ac expression levels found in the climate chamber were lower than those reported for the same cotton varieties from an Indian field study . Comparisons of Bt-expression levels remain difficult, however, since they vary with plant age and various environmental factors . Moreover, ELISA results commonly differ between studies conducted with different kits, extraction methods and purified proteins used as standards. Due to the fact that the ELISA measurements were very sensitive (with an LOD of 0.002 µg/g f.w.), it can be concluded that aphids ingest no or negligible amounts of Cry1Ac when feeding on Bt cotton even if the expression levels in the field might be higher. Consequently, negligible amounts of Cry1Ac will be passed from aphids to higher trophic levels.
In the case of phloem feeders such as aphids, the performance of natural enemies can also be influenced indirectly through the nutritional value of the honeydew . The suitability of honeydew as a sugar source can be affected by nutrient composition , as well as by the presence of secondary metabolites  or insecticidal proteins , . Honeydew sugar composition has been found to vary not only among plant species and different honeydew-producing insects , –, but also with the insect's developmental stage ,  and age , ant attendance , , the presence of bacterial symbionts in the digestive tract  as well as the rate and duration of insect infestation on the plant .
Using multivariate statistics to analyze the sugar composition of honeydew collected from A. gossypii feeding on the different varieties of Bt and non-Bt cotton, both variety and transformation were found to have a significant influence. This bioassay is the first case in which variation in honeydew sugar composition can be attributed to the latter factors. The fact that honeydew sugar composition differs among cotton varieties is not surprising given the fact that the plants generally differ in a range of parameters , . The variation in sugar composition due to transformation might be explained by differences between Bt cotton plants and their non-transformed counterparts that go beyond the intentionally introduced genes. The selection process taking place after transformation induces these additional differences in the plant material. The changes are unlikely caused by the expression of the Bt protein since it could clearly be shown that the aphids are not exposed to the insecticidal compound. The fact that more glucose was detected in honeydew collected from Bt plants (especially Bt MECH 184) compared to that from the controls may be based on variation in phloem sap composition. Alternatively, it may indicate that the carbohydrate assimilation by the aphids was somewhat affected . Either way, it is interesting that this difference did not have any influence on aphid performance. However, for a proper evaluation of the nutritional quality of the phloem sap the amino acid composition should directly be examined. In contrast to our results, Faria et al.  did not find any difference in sugar composition in aphid honeydew collected from different Bt and non-Bt maize varieties. However, the authors reported marginal differences in the amino acid composition.
Since the plant-derived sugars, mainly sucrose, glucose and fructose, are the most suitable for honeydew-feeding natural enemies , honeydew from the cotton variety MECH 184 might have increased nutritional properties compared to the other two varieties tested. However, we believe that this effect should not be overestimated since cotton features multiple nectar and extra-floral nectar sources , which are frequently exploited by parasitoids . When given a choice, parasitoids may ignore honeydew, and select extrafloral nectar. Among Microplitis croceipes (Cresson) (Hymenoptera: Braconidae) collected from fields with and without nectar sources, only individuals from fields lacking nectar contained honeydew-specific sugars (Williams and Wäckers unpublished data).
Our studies allow the conclusion that aphid performance is not affected by Cry1Ac-expressing Bt cotton plants. This, together with the fact that aphids do not ingest the insecticidal protein when feeding on Bt cotton, indicates that aphid antagonists are unlikely to be affected either directly or indirectly when attacking aphids in a Bt cotton field and that the biological control function they provide should not be compromised. This is also true for generalist predators that attack other herbivores on cotton, given the Lepidoptera-specificity of Cry1Ac , . In accordance to our laboratory studies, Bambawale et al.  observed no difference in cotton aphid abundance between Indian fields with Bt cotton (MECH 162) and its corresponding near isoline under integrated pest management. Consequently, aphids are likely to remain under biological control in Bt cotton, which is important for the sustainable deployment of this technology.
Materials and Methods
Aphis gossypii were provided by Syngenta (Stein, Switzerland) and were subsequently reared continuously on cotton (Gossypium hirsutum) in a climate chamber at 25±1°C, 70±10% r.h., and a 16-h photoperiod.
Three Bt-transgenic Gossypium hirsutum varieties (MECH 12, MECH 162, and MECH 184; event BG-I, Mahyco Seeds Ltd.), expressing the Cry1Ac protein (Bt plants) and their corresponding non-transformed near isolines (non-Bt plants) were used in the bioassays. All three Bt varieties have been commercially grown in India since 2002.
Plants were grown individually in humus-rich sterilized soil in plastic pots (3 litre) and fertilized weekly with 10N∶10P∶8K at a concentration of 20 ml/l. Plants were grown in a climate chamber, illuminated by metal halide lamps (EYE Clean-Ace lamps, MT400/DL/BH; Iwasaki Electric Co., Ltd.) at 25°C±1°C day/20°C±1°C night, 70%±10% r.h. and a 16-h photoperiod (20,000 lux during daytime). Changes between day and night conditions occurred gradually to simulate natural dusk and dawn. Metal halide lamps featured a light color close to daylight. To guarantee stable humidity, plants were placed individually in plant dishes (16 cm in diameter; 3 cm high). Plants were watered once a day until the eight-leaf stage, after which they were watered twice daily. Prior to bioassays cotton plants were controlled visually for any insect damage to avoid unintended induction of the cotton innate resistance mechanisms.
For one bioassay, 15 metal trays (45×90 cm) were placed in two rows on the floor of the climate chamber, each containing six, three-week old plants (two to five true leaves). The plants, one of each Bt and non-Bt variety, were ordered randomly per tray (complete block design). The bioassay was repeated twice resulting in a total of 30 plants per treatment. Environmental conditions were the same as described above for cultivation of the plants.
A group of approximately 50 reproductive aphids from the permanent culture were allowed to settle on a plant and to give birth to nymphs. After 6 h, two to three newborn nymphs (F1) were brushed carefully onto the last fully expanded leaf of the respective variety and covered with a clip cage (2 cm in diameter; 1 cm high). Clip cages featured a hole sealed with fine-mesh netting to provide air-circulation. After three days, surplus nymphs were removed at random, to ensure that the performance of a single nymph was monitored on each plant. Every morning and evening, aphid mortality was recorded. After reaching adulthood the F1 nymphs were counted and removed daily. This procedure was conducted until the aphid died.
The following aphid life-table parameters were obtained this way: nymphal developmental time (time from birth to first nymphal production; D), number of nymphs produced during a time span equal to D starting at nymph production (FD), mean reproductive rate during the reproductive period observed (daily fecundity; DF), total fecundity per female during the period observed (TF), adult longevity (AL), total longevity (TL), and intrinsic rate of population increase (rm). The rm was estimated based on the daily age-specific fecundity (mx) and the age-specific survival rate (lx) and using the equation of Birch  (1):(1)Aphids that died before producing any nymphs were excluded from the analysis. Aphids which were lost during the observation period were censored.
After the death of the aphid, the leaf below the one exposed to the aphid was collected and stored at −20°C until further examination. For each leaf, three trichome counts were taken from a central section of the lower surface, excluding the primary and secondary leaf veins. Depending on trichome density, measurements were done on an area of 16 or 64 mm2 (for high or low trichome density, respectively) under a binocular microscope (WILD, Heerbrugg) with the help of an ocular measuring grid (Leica). Six to eight samples were counted per treatment and subsequently data were calculated as trichome density per cm2.
Quantification of Cry1Ac protein in leaves and aphids
To confirm Cry1Ac expression of the transgenic cotton plants used in both bioassays, a total of 12 to 13 leaves per variety of the Bt plants and three leaves per variety of the non-Bt plants were collected after the end of the bioassays. Approximately 100 mg fresh weight (f.w.) of the leaves on which A. gossypii had fed were sampled, flash frozen, weighed, lyophilized and weighed again (approximately 20 mg dry weight).
To quantify the level of Cry1Ac in A. gossypii, 60 to 70 reproductive aphids were brushed on the last fully expanded leaf of three-week old Bt or non-Bt cotton plants and allowed to reproduce for five weeks. Subsequently, leaves infested with aphids were transferred to two to three-week old cotton plants of the same variety and transformation status and reared under the same climatic conditions. Aphids were allowed to settle and reproduce for one to two additional weeks. Thereafter, 30 to 80 mg of aphids was collected using a flexible tube on which gauze and a tip were attached. Before weighing and lyophilizing, each sample was checked under a binocular microscope to confirm that there was no contamination with other pests (e.g., spider mites or thrips) or leaf pieces. Three to five samples per Bt variety and one per non-Bt variety were analyzed. From the same plants on which aphids were kept, samples from two of the most heavily infested leaves were also taken for Bt protein measurements.
The amount of Cry1Ac protein in leaf and aphid material was measured using an enzyme linked immuno-sorbent assay (ELISA) from Agdia (Elkhart Indiana, USA). After adding phosphate buffered saline with Tween buffer (PBST, provided in the kit) at a ratio of 1∶10 (sample material f.w.∶buffer) and a 5 mm tungsten carbide bead, leaf samples were macerated for 100 sec at 15 Hz and aphid samples for 40 sec at 30 Hz, using a mixer mill MM300 (Retsch, Haan, Germany) fitted with 24 tube-adapters (Qiagen, Hombrechtikon, Switzerland). Samples were centrifuged for 5 min at 13,000×g and leaf samples were diluted 1∶15 with PBST, while aphid samples were not diluted. Subsequently, instructions from the kit were followed. After stopping the color development with 3 M sulfuric acid, spectrophotometric measurements were conducted with a microtiter plate reader (SpectrafluorPlus, Tecan, Männedorf, Switzerland) at 450 nm. A standard curve with purified high quality Cry1Ac provided by M. Pusztai-Carey (Dept. Biochemistry, Case Western Reserve University, Cleveland, OH, USA) was established. Concentrations were calculated using linear regression analysis. The limit of detection for aphid extracts was calculated by multiplying three times the standard deviation of eleven buffer-only and control ODs with the slope of the standard curve. Based on µg/g f.w., the limit of detection (LOD) was 0.002.
Sugar analysis of aphid honeydew
For sugar analysis, approximately 50 mg of aphids was collected from the rearing colony in a clip cage (5.2 cm in diameter; 1 cm high) in the afternoon and allowed to settle overnight on the youngest fully expanded cotton leaf. The cage was removed the following morning and the aphids were allowed to settle one additional day.
For honeydew collection, Petri dishes were placed under the aphid infested cotton leaves for 5 to 6 h. Thereafter, honeydew-sprinkled Petri dishes were placed upside down at 23±1°C and 85±5% r.h., with a water-saturated piece of cotton wool on the bottom. After 2 h, when the viscosity of the honeydew was reduced through hygroscopy, approximately 0.5 µl honeydew was collected with 5 µl micropipettes (Blaubrand, intra Mark). Subsequently, the honeydew was dissolved in 20 µl ethanol (70%) and stored at −80°C until further analysis.
For sugar analysis of aphid honeydew, five to seven samples per treatment were tested for 16 sugars: sucrose, fructose, glucose, erlose, trehalose, mannitol, sorbitol, melibiose, raffinose, stachyose, lactose, melezitose, mannose, rhamnose, maltose and galactose. Before analysis, samples were diluted 400-fold with 18 MΩ water and homogenized using a pestle. Subsequently, samples were filtered through a chromacol syringe filter. Samples were analyzed using a Dionex ICS 3000 Ion Chromatography system (Dionex Corp., Sunnyvale, CA, USA) and concentrations of the individual sugars were calculated using the program PEAKNET Software Release 5.1 as described by Steppuhn and Wäckers . The limit of quantification for any honeydew sample was set at 0.001 µg. Measurements below 0.001 µg were set to 0.
Since all assumptions were met, FD, DF, and TF were tested with a multivariate ANOVA including the factors Bt/non-Bt, variety, and experiment. Means were subsequently compared using the Tukey-Kramer Test. D, AL, and TL were analyzed by means of a Cox regression model including the factors Bt/non-Bt, variety, and experiment. Insects which were lost over the observation period were censored. To estimate the confidence intervals for the rm, the bootstrap percentile method with 10,000 resamples was performed .
Trichome density was compared among all treatments with a Kruskal-Wallis ANOVA followed by pair-wise comparisons using the Mann-Whitney U-test, adjusted for ties. The two-sided exact P-value was subsequently corrected with the Bonferroni-Holm procedure. Statistical analyses were conducted using the software package Statistica (version 6, StatSoft Inc., Tulsa, OK, USA) or R (bootstrap). The α-level was 5% in all statistical analyses.
To calculate the sugar composition of the different Bt and non-Bt varieties, firstly, the lengths of gradient was calculated. Since the value was <3, the use of linear models was justified. Therefore, an unconstrained linear ordination (indirect gradient analysis) using Principal Components Analysis (PCA) on sugar percentages (species data) was conducted to visualize the important patterns in the data. Additionally, the distribution of the sugar concentrations was investigated by constrained linear ordination (direct gradient analysis), using Redundancy Analysis (RDA) to analyze the variability between Bt and non-Bt plants and/or among varieties based on the composition of different sugars. The transformation (Bt/non-Bt) and variety (MECH 12, MECH 162, MECH 184) were used as explanatory variables. The significance of each axis of the RDA was tested using a Monte Carlo permutation test with unrestricted permutations (n = 999), followed by forward selection to determine the relative importance and significance of each environmental variable.
The software package CANOCO 4.5 was used to conduct the multivariate analysis .
We thank Ute Hagner and Natalie Tissières for helping to conduct parts of the bioassays; John-Philip Lawo for advising us with the statistical methods used and Matthias Held for supporting us with the statistical software Canoco 4.5 and providing useful advice regarding the multivariate analysis. We further thank Syngenta (Stein, Switzerland) for providing us with Aphis gossypii and Robert Hodgson for conducting the sugar analysis.
Conceived and designed the experiments: NCL FW JR. Performed the experiments: NCL. Analyzed the data: NCL JR. Contributed reagents/materials/analysis tools: FW. Wrote the paper: NCL FW JR.
- 1. Forrester NW, Cahill M, Bird L, Layland JK (1993) Management of pyrethoid and endosulfan resistance in Helicoverpa armigera (Lepidoptera: Noctuidae) in Australia. Bull Entomol Res, Supp Ser 1: 1–132.NW ForresterM. CahillL. BirdJK Layland1993Management of pyrethoid and endosulfan resistance in Helicoverpa armigera (Lepidoptera: Noctuidae) in Australia.Bull Entomol Res, Supp Ser11132
- 2. Kranthi KR, Jadhav DR, Kranthi S, Wanjari RR, Ali SS, et al. (2002) Insecticide resistance in five major insect pests of cotton in India. Crop Prot 21: 449–460.KR KranthiDR JadhavS. KranthiRR WanjariSS Ali2002Insecticide resistance in five major insect pests of cotton in India.Crop Prot21449460
- 3. Terán-Vargas AP, Rodriguez JC, Blanco CA, Martinez-Carrillo JL, Cibrian-Tovar J, et al. (2005) Bollgard cotton and resistance of tobacco budworm (Lepidoptera: Noctuidae) to conventional insecticides in Southern Tamaulipas, Mexico. J Econ Entomol 98: 2203–2209.AP Terán-VargasJC RodriguezCA BlancoJL Martinez-CarrilloJ. Cibrian-Tovar2005Bollgard cotton and resistance of tobacco budworm (Lepidoptera: Noctuidae) to conventional insecticides in Southern Tamaulipas, Mexico.J Econ Entomol9822032209
- 4. Naranjo SE, Ruberson JR, Sharma HC, Wilson L, Wu K-M (2008) The present and future role of insect-resistant GM crops in cotton IPM. In: Romeis J, Shelton AM, Kennedy GG, editors. Integration of insect-resistant GM crops within IPM programs. Dordrecht, the Netherlands: Springer. pp. 159–194.SE NaranjoJR RubersonHC SharmaL. WilsonK-M Wu2008The present and future role of insect-resistant GM crops in cotton IPM.J. RomeisAM SheltonGG KennedyIntegration of insect-resistant GM crops within IPM programsDordrecht, the NetherlandsSpringer159194
- 5. James C (2007) Global status of commercialized biotech/GM crops: 2007. ISAAA Brief No. 37. Ithaca, NY, USA: International Service for the Acquisition of Agri-Biotech Applications. C. James2007Global status of commercialized biotech/GM crops: 2007.ISAAA Brief No. 37Ithaca, NY, USAInternational Service for the Acquisition of Agri-Biotech Applications
- 6. Fitt GP (2008) Have Bt crops led to changes in insecticide use patterns and impacted IPM? In: Romeis J, Shelton AM, Kennedy GG, editors. Integration of insect-resistant GM crops within IPM programs. Dordrecht, the Netherlands: Springer. pp. 303–328.GP Fitt2008Have Bt crops led to changes in insecticide use patterns and impacted IPM?J. RomeisAM SheltonGG KennedyIntegration of insect-resistant GM crops within IPM programsDordrecht, the NetherlandsSpringer303328
- 7. Qaim M, Pray CE, Zilberman D (2008) Economic and social considerations in the adoption of Bt crops. In: Romeis J, Shelton AM, Kennedy GG, editors. Integration of insect-resistant GM crops within IPM programs. Dordrecht, the Netherlands: Springer. pp. 329–356.M. QaimCE PrayD. Zilberman2008Economic and social considerations in the adoption of Bt crops.J. RomeisAM SheltonGG KennedyIntegration of insect-resistant GM crops within IPM programsDordrecht, the NetherlandsSpringer329356
- 8. Brookes G, Barfoot P (2008) Global impact of biotech crops: Socio-economic and environmental effects, 1996–2006. AgBioForum 11: 21–38.G. BrookesP. Barfoot2008Global impact of biotech crops: Socio-economic and environmental effects, 1996–2006.AgBioForum112138
- 9. Qaim M, Subramanian A, Naik G, Zilberman D (2006) Adoption of Bt cotton and impact variability: insights from India. Rev Agric Econ 28: 48–58.M. QaimA. SubramanianG. NaikD. Zilberman2006Adoption of Bt cotton and impact variability: insights from India.Rev Agric Econ284858
- 10. Garcia-Alonso M, Jacobs E, Raybould A, Nickson TE, Sowig P, et al. (2006) A tiered system for assessing the risk of genetically modified plants to non-target organisms. Environ Biosafety Res 5: 57–65.M. Garcia-AlonsoE. JacobsA. RaybouldTE NicksonP. Sowig2006A tiered system for assessing the risk of genetically modified plants to non-target organisms.Environ Biosafety Res55765
- 11. Romeis J, Bartsch D, Bigler F, Candolfi MP, Gielkens MMC, et al. (2008) Assessment of risk of insect-resistant transgenic crops to nontarget arthropods. Nat Biotech 26: 203–208.J. RomeisD. BartschF. BiglerMP CandolfiMMC Gielkens2008Assessment of risk of insect-resistant transgenic crops to nontarget arthropods.Nat Biotech26203208
- 12. Romeis J, Meissle M, Bigler F (2006) Transgenic crops expressing Bacillus thuringiensis toxins and biological control. Nat Biotech 24: 63–71.J. RomeisM. MeissleF. Bigler2006Transgenic crops expressing Bacillus thuringiensis toxins and biological control.Nat Biotech246371
- 13. Wolfenbarger LL, Naranjo SE, Lundgren JG, Bitzer RJ, Watrud LS (2008) Bt crop effects on functional guilds of non-target arthropods: a meta-analysis. PLoS ONE 3(5): e2118 18461164.e2118.LL WolfenbargerSE NaranjoJG LundgrenRJ BitzerLS Watrud2008Bt crop effects on functional guilds of non-target arthropods: a meta-analysis.PLoS ONE3(5)e2118 18461164.e2118
- 14. Schuler TH, Denholm I, Jouanin L, Clark SJ, Clark AJ, et al. (2001) Population-scale laboratory studies of the effect of transgenic plants on nontarget insects. Mol Ecol 10: 1845–1853.TH SchulerI. DenholmL. JouaninSJ ClarkAJ Clark2001Population-scale laboratory studies of the effect of transgenic plants on nontarget insects.Mol Ecol1018451853
- 15. Dutton A, Klein H, Romeis J, Bigler F (2002) Uptake of Bt-toxin by herbivores feeding on transgenic maize and consequences for the predator Chrysoperla carnea. Ecol Entomol 27: 441–447.A. DuttonH. KleinJ. RomeisF. Bigler2002Uptake of Bt-toxin by herbivores feeding on transgenic maize and consequences for the predator Chrysoperla carnea.Ecol Entomol27441447
- 16. Raps A, Kehr J, Gugerli P, Moar WJ, Bigler F, et al. (2001) Immunological analysis of phloem sap of Bacillus thuringiensis corn and of the nontarget herbivore Rhopalosiphum padi (Homoptera: Aphididae) for the presence of Cry1Ab. Mol Ecol 10: 525–533.A. RapsJ. KehrP. GugerliWJ MoarF. Bigler2001Immunological analysis of phloem sap of Bacillus thuringiensis corn and of the nontarget herbivore Rhopalosiphum padi (Homoptera: Aphididae) for the presence of Cry1Ab.Mol Ecol10525533
- 17. Head G, Brown CR, Groth ME, Duan JJ (2001) Cry1Ab protein levels in phytophagous insects feeding on transgenic corn: implications for secondary exposure risk assessment. Entomol Exp Appl 99: 37–45.G. HeadCR BrownME GrothJJ Duan2001Cry1Ab protein levels in phytophagous insects feeding on transgenic corn: implications for secondary exposure risk assessment.Entomol Exp Appl993745
- 18. Schuler TH, Clark AJ, Clark SJ, Poppy GM, Stewart JCN, et al. (2005) Laboratory studies of the effects of reduced prey choice caused by Bt plants on a predatory insect. Bull Entomol Res 95: 243–247.TH SchulerAJ ClarkSJ ClarkGM PoppyJCN Stewart2005Laboratory studies of the effects of reduced prey choice caused by Bt plants on a predatory insect.Bull Entomol Res95243247
- 19. Obrist LB, Dutton A, Albajes R, Bigler F (2006) Exposure of arthropod predators to Cry1Ab toxin in Bt maize fields. Ecol Entomol 31: 134–154.LB ObristA. DuttonR. AlbajesF. Bigler2006Exposure of arthropod predators to Cry1Ab toxin in Bt maize fields.Ecol Entomol31134154
- 20. Torres JB, Ruberson JR, Adang MJ (2006) Expression of Bacillus thuringiensis Cry1Ac protein in cotton plants, acquisition by pests and predators: A tritrophic analysis. Agric Forest Entomol 8: 191–202.JB TorresJR RubersonMJ Adang2006Expression of Bacillus thuringiensis Cry1Ac protein in cotton plants, acquisition by pests and predators: A tritrophic analysis.Agric Forest Entomol8191202
- 21. Zhang G-F, Wan F-H, Lövei GL, Liu W-X, Guo J-Y (2006) Transmission of Bt toxin to the predator Propylaea japonica (Coleoptera: Coccinellidae) through its aphid prey feeding on transgenic Bt cotton. Environ Entomol 35: 143–150.G-F ZhangF-H WanGL LöveiW-X LiuJ-Y Guo2006Transmission of Bt toxin to the predator Propylaea japonica (Coleoptera: Coccinellidae) through its aphid prey feeding on transgenic Bt cotton.Environ Entomol35143150
- 22. Burgio G, Lanzoni A, Accinelli G, Dinelli G, Bonetti A, et al. (2007) Evaluation of Bt-toxin uptake by the non-target herbivore, Myzus persicae (Hemiptera: Aphididae), feeding on transgenic oilseed rape. Bull Entomol Res 97: 211–215.G. BurgioA. LanzoniG. AccinelliG. DinelliA. Bonetti2007Evaluation of Bt-toxin uptake by the non-target herbivore, Myzus persicae (Hemiptera: Aphididae), feeding on transgenic oilseed rape.Bull Entomol Res97211215
- 23. Wäckers FL (2005) Suitability of (extra-)floral nectar, pollen, and honeydew as insect food sources. In: Wäckers FL, van Rijn PCJ, Bruin J, editors. Plant-provided food for carnivorous insects. Cambridge, UK: Cambridge University Press. pp. 17–74.FL Wäckers2005Suitability of (extra-)floral nectar, pollen, and honeydew as insect food sources.FL WäckersPCJ van RijnJ. BruinPlant-provided food for carnivorous insectsCambridge, UKCambridge University Press1774
- 24. Hogervorst PAM, Wäckers FL, Romeis J (2007) Detecting nutritional state and food source use in field-collected insects that synthesize honeydew oligosaccharides. Funct Ecol 21: 936–946.PAM HogervorstFL WäckersJ. Romeis2007Detecting nutritional state and food source use in field-collected insects that synthesize honeydew oligosaccharides.Funct Ecol21936946
- 25. Fadamiro HY, Heimpel GE (2001) Effects of partial sugar deprivation on lifespan and carbohydrate mobilization in the parasitoid Macrocentrus grandii (Hymenoptera: Braconidae). Ann Entomol Soc Amer 94: 909–916.HY FadamiroGE Heimpel2001Effects of partial sugar deprivation on lifespan and carbohydrate mobilization in the parasitoid Macrocentrus grandii (Hymenoptera: Braconidae).Ann Entomol Soc Amer94909916
- 26. Siekmann G, Tenhumberg B, Keller MA (2001) Feeding and survival in parasitic wasps: Sugar concentration and timing matter. Oikos 95: 425–430.G. SiekmannB. TenhumbergMA Keller2001Feeding and survival in parasitic wasps: Sugar concentration and timing matter.Oikos95425430
- 27. Wäckers FL (2001) A comparison of nectar- and honeydew sugars with respect to their utilization by the hymenopteran parasitoid Cotesia glomerata. J Insect Physiol 47: 1077–1084.FL Wäckers2001A comparison of nectar- and honeydew sugars with respect to their utilization by the hymenopteran parasitoid Cotesia glomerata.J Insect Physiol4710771084
- 28. Winkler K, Wäckers FL, Bukovinszkine-Kiss G, van Lenteren JC (2006) Sugar resources are vital for Diadegma semiclausum fecundity under field conditions. Basic Appl Ecol 7: 133–140.K. WinklerFL WäckersG. Bukovinszkine-KissJC van Lenteren2006Sugar resources are vital for Diadegma semiclausum fecundity under field conditions.Basic Appl Ecol7133140
- 29. Hogervorst PAM, Wäckers F, Romeis J (2007) Effects of honeydew sugar composition on the longevity of Aphidius ervi. Entomol Exp Appl 122: 223–232.PAM HogervorstF. WäckersJ. Romeis2007Effects of honeydew sugar composition on the longevity of Aphidius ervi.Entomol Exp Appl122223232
- 30. Wäckers FL, van Rijn PCJ, Heimpel GE (2008) Honeydew as a food source for natural enemies: Making the best of a bad meal? Biol Contr 45: 176–184.FL WäckersPCJ van RijnGE Heimpel2008Honeydew as a food source for natural enemies: Making the best of a bad meal?Biol Contr45176184
- 31. Du L, Feng GE, Sanrong Z, Parajulee MN (2004) Effect of cotton cultivar on development and reproduction of Aphis gossypii (Homoptera: Aphididae) and its predator Propylaea japonica (Coleoptera: Coccinellidae). J Econ Entomol 97: 1278–1283.L. DuGE FengZ. SanrongMN Parajulee2004Effect of cotton cultivar on development and reproduction of Aphis gossypii (Homoptera: Aphididae) and its predator Propylaea japonica (Coleoptera: Coccinellidae).J Econ Entomol9712781283
- 32. Weathersbee AA, Hardee DD (1994) Abundance of cotton aphids (Homoptera, Aphididae) and associated biological control agents on six cotton cultivars. J Econ Entomol 87: 258–265.AA WeathersbeeDD Hardee1994Abundance of cotton aphids (Homoptera, Aphididae) and associated biological control agents on six cotton cultivars.J Econ Entomol87258265
- 33. Xue K, Deng S, Wang RJ, Yan FM, Xu CR (2008) Leaf surface factors of transgenic Bt cotton associated with the feeding behaviors of cotton aphids: A case study on non-target effects. Sci China Ser C, Life Sci 51: 145–156.K. XueS. DengRJ WangFM YanCR Xu2008Leaf surface factors of transgenic Bt cotton associated with the feeding behaviors of cotton aphids: A case study on non-target effects.Sci China Ser C, Life Sci51145156
- 34. Liu XD, Zhai BP, Zhang XX, Zong JM (2005) Impact of transgenic cotton plants on a non-target pest, Aphis gossypii Glover. Ecol Entomol 30: 307–315.XD LiuBP ZhaiXX ZhangJM Zong2005Impact of transgenic cotton plants on a non-target pest, Aphis gossypii Glover.Ecol Entomol30307315
- 35. Bambawale OM, Singh A, Sharma OP, Bhosle BB, Lavekar RC, et al. (2004) Performance of Bt cotton (MECH-162) under integrated pest management in farmers' participatory field trail in Nanded district, Central India. Curr Sci 86: 1628–1633.OM BambawaleA. SinghOP SharmaBB BhosleRC Lavekar2004Performance of Bt cotton (MECH-162) under integrated pest management in farmers' participatory field trail in Nanded district, Central India.Curr Sci8616281633
- 36. Mellet MA, Schoeman AS (2007) Effect of Bt-cotton on chrysopids, ladybird beetles and their prey: Aphids and whiteflies. Indian J Exp Biol 45: 554–562.MA MelletAS Schoeman2007Effect of Bt-cotton on chrysopids, ladybird beetles and their prey: Aphids and whiteflies.Indian J Exp Biol45554562
- 37. Zhang G-F, Wan F-H, Murphy ST, Guo J-Y, Liu W-X (2008) Reproductive biology of two nontarget insect species, Aphis gossypii (Homoptera: Aphididae) and Orius sauteri (Hemiptera: Anthocoridae), on Bt and non-Bt cotton cultivars. Environ Entomol 37: 1035–1042.G-F ZhangF-H WanST MurphyJ-Y GuoW-X Liu2008Reproductive biology of two nontarget insect species, Aphis gossypii (Homoptera: Aphididae) and Orius sauteri (Hemiptera: Anthocoridae), on Bt and non-Bt cotton cultivars.Environ Entomol3710351042
- 38. Cui J, Xia J (2000) Effects of Bt (Bacillus thuringiensis) transgenic cotton on the dynamics of pest population and their enemies. Acta Phytophyl Sinica 27: 141–145.J. CuiJ. Xia2000Effects of Bt (Bacillus thuringiensis) transgenic cotton on the dynamics of pest population and their enemies.Acta Phytophyl Sinica27141145
- 39. Deng SD, Xu J, Zhang QW, Zhou SW, Xu GJ (2003) Effect of transgenic Bt cotton on population dynamics of the non-target pests and natural enemies of pests. Acta Entomol Sinica (in Chineses) 46: 1–5.SD DengJ. XuQW ZhangSW ZhouGJ Xu2003Effect of transgenic Bt cotton on population dynamics of the non-target pests and natural enemies of pests.Acta Entomol Sinica (in Chineses)4615
- 40. Wu KM, Guo YY (2003) Influences of Bacillus thuringiensis Berliner cotton planting on population dynamics of the cotton aphid, Aphis gossypii Glover, in northern China. Environ Entomol 32: 312–318.KM WuYY Guo2003Influences of Bacillus thuringiensis Berliner cotton planting on population dynamics of the cotton aphid, Aphis gossypii Glover, in northern China.Environ Entomol32312318
- 41. Romeis J, Meissel M, Raybould A, Hellmich RL (2009) Impact of insect-resistant genetically modified crops on above-ground non-target arthropods. In: Ferry N, Gatehouse AMR, editors. Environmental impact of genetically modified crops. Wallingford, UK: CABI. pp. 165–198.J. RomeisM. MeisselA. RaybouldRL Hellmich2009Impact of insect-resistant genetically modified crops on above-ground non-target arthropods.N. FerryAMR GatehouseEnvironmental impact of genetically modified cropsWallingford, UKCABI165198
- 42. Faria CA, Wäckers FL, Pritchard J, Barrett DA, Turlings TCJ (2007) High susceptibility of Bt maize to aphids enhances the performance of parasitoids of lepidopteran pests. PLoS ONE 7(2): e600.CA FariaFL WäckersJ. PritchardDA BarrettTCJ Turlings2007High susceptibility of Bt maize to aphids enhances the performance of parasitoids of lepidopteran pests.PLoS ONE7(2)e600
- 43. Lumbierres B, Albajes R, Pons X (2004) Transgenic Bt maize and Rhopalosiphum padi (Hom., Aphididae) performance. Ecol Entomol 29: 309–317.B. LumbierresR. AlbajesX. Pons2004Transgenic Bt maize and Rhopalosiphum padi (Hom., Aphididae) performance.Ecol Entomol29309317
- 44. Lozzia GC, Furlanis C, Manachini B, Rigamonti IE (1998) Effects of Bt corn on Rhopalosiphum padi L. (Rhynchota: Aphidae) and on its predator Chrysoperla carnea Stephen (Neuroptera Chrysopidae). Boll Zool agr Bachic 30: 153–164.GC LozziaC. FurlanisB. ManachiniIE Rigamonti1998Effects of Bt corn on Rhopalosiphum padi L. (Rhynchota: Aphidae) and on its predator Chrysoperla carnea Stephen (Neuroptera Chrysopidae).Boll Zool agr Bachic30153164
- 45. Ramirez-Romero R, Desneux N, Chaufaux J, Kaiser L (2008) Bt-maize effects on biological parameters of the non-target aphid Sitobion avenae (Homoptera: Aphididae) and Cry1Ab toxin detection. Pest Biochem Physiol 91: 110–115.R. Ramirez-RomeroN. DesneuxJ. ChaufauxL. Kaiser2008Bt-maize effects on biological parameters of the non-target aphid Sitobion avenae (Homoptera: Aphididae) and Cry1Ab toxin detection.Pest Biochem Physiol91110115
- 46. Bourguet D, Chaufaux J, Micoud A, Delos M, Naibo B, et al. (2002) Ostrinia nubilalis parasitism and the field abundance of non-target insects in transgenic Bacillus thuringiensis corn (Zea mays). Environ Biosafety Res 1: 49–60.D. BourguetJ. ChaufauxA. MicoudM. DelosB. Naibo2002Ostrinia nubilalis parasitism and the field abundance of non-target insects in transgenic Bacillus thuringiensis corn (Zea mays).Environ Biosafety Res14960
- 47. Pons X, Lumbierres B, Lopez C, Albajes R (2005) Abundance of non-target pests in transgenic Bt-maize: A farm scale study. Eur J Entomol 102: 73–79.X. PonsB. LumbierresC. LopezR. Albajes2005Abundance of non-target pests in transgenic Bt-maize: A farm scale study.Eur J Entomol1027379
- 48. Bernal CC, Aguda RM, Cohen MB (2002) Effect of rice lines transformed with Bacillus thuringiensis toxin genes on the brown planthopper and its predator Cyrtorhinus lividipennis. Entomol Exp Appl 102: 21–28.CC BernalRM AgudaMB Cohen2002Effect of rice lines transformed with Bacillus thuringiensis toxin genes on the brown planthopper and its predator Cyrtorhinus lividipennis.Entomol Exp Appl1022128
- 49. Bai YY, Jiang MX, Cheng JA, Wang D (2006) Effects of CrylAb toxin on Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) through its prey, Nilaparvata lugens Stål (Homoptera: Delphacidae), feeding on transgenic Bt rice. Environ Entomol 35: 1130–1136.YY BaiMX JiangJA ChengD. Wang2006Effects of CrylAb toxin on Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) through its prey, Nilaparvata lugens Stål (Homoptera: Delphacidae), feeding on transgenic Bt rice.Environ Entomol3511301136
- 50. Obrist LB, Klein H, Dutton A, Bigler F (2005) Effects of Bt maize on Frankliniella tenuicornis and exposure of thrips predators to prey-mediated Bt toxins. Entomol Exp Appl 115: 409–416.LB ObristH. KleinA. DuttonF. Bigler2005Effects of Bt maize on Frankliniella tenuicornis and exposure of thrips predators to prey-mediated Bt toxins.Entomol Exp Appl115409416
- 51. Torres JB, Ruberson JR (2008) Interactions of Bacillus thuringiensis Cry1Ac toxin in genetically engineered cotton with predatory heteropterans. Trans Res 17: 345–354.JB TorresJR Ruberson2008Interactions of Bacillus thuringiensis Cry1Ac toxin in genetically engineered cotton with predatory heteropterans.Trans Res17345354
- 52. Kranthi KR, Naidu S, Dhawad CS, Tatwawadi A, Mate K, et al. (2005) Temporal and intra-plant variability of Cry1Ac expression in Bt-cotton and its influence on the survival of the cotton bollworm, Helicoverpa armigera (Hübner) (Noctuidae: Lepidoptera). Curr Sci 89: 291–298.KR KranthiS. NaiduCS DhawadA. TatwawadiK. Mate2005Temporal and intra-plant variability of Cry1Ac expression in Bt-cotton and its influence on the survival of the cotton bollworm, Helicoverpa armigera (Hübner) (Noctuidae: Lepidoptera).Curr Sci89291298
- 53. Dong HZ, Li WJ (2007) Variability of endotoxin expression in Bt transgenic cotton. J Agron&Crop Sci 193: 21–29.HZ DongWJ Li2007Variability of endotoxin expression in Bt transgenic cotton.J Agron&Crop Sci1932129
- 54. Wäckers FL (2000) Do oligosaccharides reduce the suitability of honeydew for predators and parasitoids? A further facet to the function of insect-synthesized honeydew sugars. Oikos 90: 197–201.FL Wäckers2000Do oligosaccharides reduce the suitability of honeydew for predators and parasitoids? A further facet to the function of insect-synthesized honeydew sugars.Oikos90197201
- 55. Romeis J, Babendreier D, Wäckers FL (2003) Consumption of snowdrop lectin (Galanthus nivalis agglutinin) causes direct effects on adult parasitic wasps. Oecologia 134: 528–536.J. RomeisD. BabendreierFL Wäckers2003Consumption of snowdrop lectin (Galanthus nivalis agglutinin) causes direct effects on adult parasitic wasps.Oecologia134528536
- 56. Hogervorst PAM, Wäckers FL, Woodring J, Romeis J (2009) Snowdrop lectin (Galanthus nivalis agglutinin) in aphid honeydew negatively affects survival of a honeydew-consuming parasitoid. Agric Forest Entomol 11: in press.PAM HogervorstFL WäckersJ. WoodringJ. Romeis2009Snowdrop lectin (Galanthus nivalis agglutinin) in aphid honeydew negatively affects survival of a honeydew-consuming parasitoid.Agric Forest Entomol11in press
- 57. Byrne DN, Miller WB (1990) Carbohydrate and amino-acid-composition of phloem sap and honeydew produced by Bemisia tabaci. J Insect Physiol 36: 433–439.DN ByrneWB Miller1990Carbohydrate and amino-acid-composition of phloem sap and honeydew produced by Bemisia tabaci.J Insect Physiol36433439
- 58. Hendrix DL, Wei YA, Leggett JE (1992) Homopteran honeydew sugar composition is determined by both the insect and plant-species. Comp Biochem Physiol B-Biochem Mol Biol 101: 23–27.DL HendrixYA WeiJE Leggett1992Homopteran honeydew sugar composition is determined by both the insect and plant-species.Comp Biochem Physiol B-Biochem Mol Biol1012327
- 59. Völkl W, Woodring J, Fischer M, Lorenz MW, Hoffmann KH (1999) Ant-aphid mutualisms: the impact of honeydew production and honeydew sugar composition on ant preferences. Oecologia 118: 483–491.W. VölklJ. WoodringM. FischerMW LorenzKH Hoffmann1999Ant-aphid mutualisms: the impact of honeydew production and honeydew sugar composition on ant preferences.Oecologia118483491
- 60. Arakaki N, Hattori M (1998) Differences in the quality and quantity of honeydew from first instar soldier and ordinary morph nymphs of the bamboo aphid, Pseudoregma koshunensis (Takahashi) (Homoptera: Aphididae). Appl Entomol Zool 33: 357–361.N. ArakakiM. Hattori1998Differences in the quality and quantity of honeydew from first instar soldier and ordinary morph nymphs of the bamboo aphid, Pseudoregma koshunensis (Takahashi) (Homoptera: Aphididae).Appl Entomol Zool33357361
- 61. Costa HS, Toscano NC, Hendrix DL, Henneberry TJ (1999) Patterns of honeydew droplet production by nymphal stages of Bemisia argentifolii (Homoptera: Aleyrodidae) and relative composition of honeydew sugars. J Entomol Sci 34: 305–313.HS CostaNC ToscanoDL HendrixTJ Henneberry1999Patterns of honeydew droplet production by nymphal stages of Bemisia argentifolii (Homoptera: Aleyrodidae) and relative composition of honeydew sugars.J Entomol Sci34305313
- 62. Fischer MK, Völkl W, Schopf R, Hoffmann KH (2002) Age-specific patterns in honeydew production and honeydew composition in the aphid Metopeurum fuscoviride: implications for ant-attendance. J Insect Physiol 48: 319–326.MK FischerW. VölklR. SchopfKH Hoffmann2002Age-specific patterns in honeydew production and honeydew composition in the aphid Metopeurum fuscoviride: implications for ant-attendance.J Insect Physiol48319326
- 63. Fischer MK, Shingleton AW (2001) Host plant and ants influence the honeydew sugar composition of aphids. Funct Ecol 15: 544–550.MK FischerAW Shingleton2001Host plant and ants influence the honeydew sugar composition of aphids.Funct Ecol15544550
- 64. Yao I, Akimoto S (2001) Ant attendance changes the sugar composition of the honeydew of the drepanosiphid aphid Tuberculatus quercicola. Oecologia 128: 36–43.I. YaoS. Akimoto2001Ant attendance changes the sugar composition of the honeydew of the drepanosiphid aphid Tuberculatus quercicola.Oecologia1283643
- 65. Wilkinson TL, Ashford DA, Pritchard J, Douglas AE (1997) Honeydew sugars and osmoregulation in the pea aphid Acyrthosiphon pisum. J Exp Biol 200: 2137–2143.TL WilkinsonDA AshfordJ. PritchardAE Douglas1997Honeydew sugars and osmoregulation in the pea aphid Acyrthosiphon pisum.J Exp Biol20021372143
- 66. Faria CA, Wäckers F, Turlings TCJ (2008) The nutritional value of aphid honeydew for non-aphid parasitoids. Basic Appl Ecol 9: 286–297.CA FariaF. WäckersTCJ Turlings2008The nutritional value of aphid honeydew for non-aphid parasitoids.Basic Appl Ecol9286297
- 67. Ashford DA, Smith WA, Douglas AE (2000) Living on a high sugar diet: the fate of sucrose ingested by a phloem-feeding insect, the pea aphid Acyrthosiphon pisum. J Insect Physiol 46: 335–341.DA AshfordWA SmithAE Douglas2000Living on a high sugar diet: the fate of sucrose ingested by a phloem-feeding insect, the pea aphid Acyrthosiphon pisum.J Insect Physiol46335341
- 68. Wäckers FL, Bonifay C (2004) How to be sweet? Extrafloral nectar allocation in Gossypium hirsutum fits optimal defense theory predictions. Ecology 85: 1512–1518.FL WäckersC. Bonifay2004How to be sweet? Extrafloral nectar allocation in Gossypium hirsutum fits optimal defense theory predictions.Ecology8515121518
- 69. Stapel JO, Cortesero AM, de Moraes CM, Tumlinson JH, Lewis WJ (1997) Extrafloral nectar, honeydew and sucrose effects on searching behavior and efficiency of Microplitis croceipes (Hymenoptera: Braconidae) in cotton. Environ Entomol 26: 617–623.JO StapelAM CorteseroCM de MoraesJH TumlinsonWJ Lewis1997Extrafloral nectar, honeydew and sucrose effects on searching behavior and efficiency of Microplitis croceipes (Hymenoptera: Braconidae) in cotton.Environ Entomol26617623
- 70. Birch LC (1948) The intrinsic rate of natural increase of an insect population. J Animal Ecol 17: 15–26.LC Birch1948The intrinsic rate of natural increase of an insect population.J Animal Ecol171526
- 71. Steppuhn A, Wäckers FL (2004) HPLC sugar analysis reveals the nutritional state and the feeding history of parasitoids. Funct Ecol 18: 812–819.A. SteppuhnFL Wäckers2004HPLC sugar analysis reveals the nutritional state and the feeding history of parasitoids.Funct Ecol18812819
- 72. Davison AC, Hinkley DV (1997) Bootstrap methods and their applications. Cambridge: Cambridge University Press. AC DavisonDV Hinkley1997Bootstrap methods and their applicationsCambridgeCambridge University Press203
- 73. ter Braak CJF, Šmilauer P (2002) CANOCO Reference manual and CanoDraw for Windows User's guide: Software for Canonical community Ordination (version 4.5). Ithaca, NY, USA: Microcomputer Power. CJF ter BraakP. Šmilauer2002CANOCO Reference manual and CanoDraw for Windows User's guide: Software for Canonical community Ordination (version 4.5)Ithaca, NY, USAMicrocomputer Power